Addgene pGDP3 apmA Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: pGDP3 apmA Sequencing Result


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BbsI (3942) BsgI * (3906) BspEI * (3871) BsaBI * (3867) AfeI (3810) PvuII (3473) pGEX 3' (3418 .. 3440) PfoI (3417) BsaAI (3312) BstZ17I (3293) AccI (3292) pRS-marker (3258 .. 3277) BspQI - SapI (3180) L4440 (3161 .. 3178) AflIII - PciI (3063) pBR322ori-F (2908 .. 2927) PspFI (2763) BseYI (2759) AlwNI (2654) AhdI (2175) BsaI (2109) BsrFI (2090) BglI (2057) NmeAIII (2028) BglII (30) XbaI (220) BtgI - NcoI - StyI (259) 6xHis NdeI (319) BspDI - ClaI (690) BsmI (885) NsiI (887) AvaI - BsoBI - PaeR7I - PspXI - XhoI (1146) 6xHis BlpI (1225) rrnB T2 terminator Amp-R (1606 .. 1625) BsaHI (1635) ScaI (1694) PstI (1933) FspI (1952) AseI (2000) pGDP3 apmA 4027 bp

Sequence View

BglII 60 a g t a a g g c a a c c c c g c c a g c c t a g c c g g g a g a t c t g g c c t a t t g g t t a a a a a a t g a g c t g t c a t t c c g t t g g g g c g g t c g g a t c g g c c c t c t a g a c c g g a t a a c c a a t t t t t t a c t c g a c

120 a t t t a a c a a a a a t t t a a c g c g a a t t t t a a c a a a a t a t t a a c g c t t a c a a t t t a g g t g g c a t a a a t t g t t t t t a a a t t g c g c t t a a a a t t g t t t t a t a a t t g c g a a t g t t a a a t c c a c c g t

180 c t t t t c g g g g a a a t g t g c g c g g a a c c c c t a t t t g t t t a t t t t t c t a a a t a c a t t c a a a t a g a a a a g c c c c t t t a c a c g c g c c t t g g g g a t a a a c a a a t a a a a a g a t t t a t g t a a g t t t a t

XbaI 240 RBS t g t a t c c g c t c a t g a g a c a a t a a c c c t g a t a a a t g c t t c t c t a g a a a t a a t t t t g t t t a a a c a t a g g c g a g t a c t c t g t t a t t g g g a c t a t t t a c g a a g a g a t c t t t a t t a a a a c a a a t t

NcoIStyIBtgI 300 1 5 HHHHHH SSG RBS 6xHis thrombin site MGSSHHHHHHSSG c t t t a a g a a g g a g a t a t a c c a t g g g c a g c a g c c a t c a t c a t c a t c a t c a c a g c a g c g g c c g a a a t t c t t c c t c t a t a t g g t a c c c g t c g t c g g t a g t a g t a g t a g t a g t g t c g t c g c c g g

NdeI 360 1 5 LVPRGS (in frame with thrombin site) HMKTRLEQVLERYL thrombin site LVPRGSHMKTRLEQVLERYL t g g t g c c g c g c g g c a g c c a t a t g a a a a c c a g a c t t g a a c a a g t t t t a g a a c g t t a t c t c a a c c a c g g c g c g c c g t c g g t a t a c t t t t g g t c t g a a c t t g t t c a a a a t c t t g c a a t a g a g t

420 (in frame with thrombin site) NGREVAVWGVPTRRLLRALK NGREVAVWGVPTRRLLRALK a c g g a c g c g a g g t a g c t g t a t g g g g t g t t c c g a c a c g c c g t t t g c t t c g t g c a t t a a a a c t g c c t g c g c t c c a t c g a c a t a c c c c a c a a g g c t g t g c g g c a a a c g a a g c a c g t a a t t t t g

480 (in frame with thrombin site) PFKFHTADRVDPQYHYVVAV PFKFHTADRVDPQYHYVVAV c t t t t a a a t t t c a t a c t g c c g a t c g t g t t g a t c c t c a a t a t c a t t a t g t t g t t g c t g t a a g a a a a t t t a a a g t a t g a c g g c t a g c a c a a c t a g g a g t t a t a g t a a t a c a a c a a c g a c a t t

540 (in frame with thrombin site) TDDDLTDFLSDEQSKSFQYA TDDDLTDFLSDEQSKSFQYA c c g a t g a c g a c t t a a c c g a c t t t t t a t c c g a t g a a c a g a g c a a g t c g t t t c a a t a c g c g a g g c t a c t g c t g a a t t g g c t g a a a a a t a g g c t a c t t g t c t c g t t c a g c a a a g t t a t g c g c t

600 (in frame with thrombin site) NDYLTFDDEGGELPFERMCF NDYLTFDDEGGELPFERMCF a t g a c t a c c t t a c g t t t g a c g a c g a g g g a g g c g a a c t t c c g t t t g a a c g a a t g t g c t t c a t a c t g a t g g a a t g c a a a c t g c t g c t c c c t c c g c t t g a a g g c a a a c t t g c t t a c a c g a a g t

660 (in frame with thrombin site) NVPVGRQTYFGDGVVGACEN NVPVGRQTYFGDGVVGACEN a t g t c c c c g t t g g c a g g c a a a c g t a t t t t g g a g a c g g t g t c g t a g g a g c t t g c g a a a a c g t a c a g g g g c a a c c g t c c g t t t g c a t a a a a c c t c t g c c a c a g c a t c c t c g a a c g c t t t t g c

BspDIClaI 720 (in frame with thrombin site) GYIKSIGQFTSINGTAEIHA GYIKSIGQFTSINGTAEIHA g g t a t a t c a a g a g c a t c g g c c a a t t t a c a t c g a t t a a c g g c a c g g c a g a g a t c c a t g c g a c c a t a t a g t t c t c g t a g c c g g t t a a a t g t a g c t a a t t g c c g t g c c g t c t c t a g g t a c g c t

780 (in frame with thrombin site) NHQLNMTFVSDDIQNFFNEE NHQLNMTFVSDDIQNFFNEE a c c a t c a g t t a a a c a t g a c c t t t g t a a g c g a c g a t a t t c a a a a c t t c t t c a a c g a a g a a a t g g t a g t c a a t t t g t a c t g g a a a c a t t c g c t g c t a t a a g t t t t g a a g a a g t t g c t t c t t t

840 (in frame with thrombin site) SMAVFQEKLRKDPKHPYAYS SMAVFQEKLRKDPKHPYAYS g c a t g g c t g t a t t c c a a g a a a a g c t g c g a a a a g a c c c g a a a c a c c c t t a t g c t t a t a g c a c g t a c c g a c a t a a g g t t c t t t t c g a c g c t t t t c t g g g c t t t g t g g g a a t a c g a a t a t c g t

BsmINsiI 900 (in frame with thrombin site) KEPMTIGSDVYIGAHAFINA KEPMTIGSDVYIGAHAFINA a a g a a c c t a t g a c c a t a g g t a g c g a t g t t t a t a t c g g c g c a c a t g c a t t c a t c a a t g c t t t t c t t g g a t a c t g g t a t c c a t c g c t a c a a a t a t a g c c g c g t g t a c g t a a g t a g t t a c g a a

960 (in frame with thrombin site) STVTSIGDGAIIGSGAVVLE STVTSIGDGAIIGSGAVVLE c c a c t g t g a c a a g c a t c g g t g a c g g t g c a a t a a t t g g c t c a g g g g c a g t a g t t c t g g a a a g g t g a c a c t g t t c g t a g c c a c t g c c a c g t t a t t a a c c g a g t c c c c g t c a t c a a g a c c t t t

1020 (in frame with thrombin site) NVPPFAVVVGVPARIKRYRF NVPPFAVVVGVPARIKRYRF a t g t t c c t c c t t t t g c t g t c g t c g t t g g t g t c c c t g c g a g a a t c a a g c g t t a c c g c t t c t t a c a a g g a g g a a a a c g a c a g c a g c a a c c a c a g g g a c g c t c t t a g t t c g c a a t g g c g a a g a

1080 (in frame with thrombin site) SKEMIETLLRVKWWDWSIEE SKEMIETLLRVKWWDWSIEE c a a a g g a g a t g a t t g a a a c c c t c c t t c g t g t c a a a t g g t g g g a c t g g a g t a t a g a g g a a a g t t t c c t c t a c t a a c t t t g g g a g g a a g c a c a g t t t a c c a c c c t g a c c t c a t a t c t c c t t t

1140 (in frame with thrombin site) INENVDALISPELFMKKYGS INENVDALISPELFMKKYGS t t a a c g a a a a t g t t g a t g c g c t t a t a t c a c c t g a a c t a t t t a t g a a a a a g t a c g g g a g t t a a t t g c t t t t a c a a c t a c g c g a a t a t a g t g g a c t t g a t a a a t a c t t t t t c a t g c c c t c a a

PaeR7IBsoBIPspXIXhoIAvaI 1200 L* 1 5 HHHHHH * 6xHis L* t g t a a c t c g a g c a c c a c c a c c a c c a c c a c t g a g a t c c g g c t g c t a a c a a a g c c c g a a a g g a c a t t g a g c t c g t g g t g g t g g t g g t g g t g a c t c t a g g c c g a c g a t t g t t t c g g g c t t t c c

BlpI 1260 rrnB T2 terminator a a g c t g a g t t g g c t g c t g c c a c c g c t g a g c a a a a g g c c a t c c g t c a g g a t g g c c t t c t g g t t c g a c t c a a c c g a c g a c g g t g g c g a c t c g t t t t c c g g t a g g c a g t c c t a c c g g a a g a c c

1320 AmpR promoter t g g c a c t t t t c g g g g a a a t g t g c g c g g a a c c c c t a t t t g t t t a t t t t t c t a a a t a c a t t c a c c g t g a a a a g c c c c t t t a c a c g c g c c t t g g g g a t a a a c a a a t a a a a a g a t t t a t g t a a g

1380 AmpR promoter a a a t a t g t a t c c g c t c a t g a g a c a a t a a c c c t g a t a a a t g c t t c a a t a a t a t t g a a a a a g t t t a t a c a t a g g c g a g t a c t c t g t t a t t g g g a c t a t t t a c g a a g t t a t t a t a a c t t t t t c

1440 1 5 10 15 signal sequence MSIQHFRVALIPFFAAFC AmpR promoter AmpR MSIQHFRVALIPFFAAFC g a a g a g t a t g a g t a t t c a a c a t t t c c g t g t c g c c c t t a t t c c c t t t t t t g c g g c a t t t t g c t t c t c a t a c t c a t a a g t t g t a a a g g c a c a g c g g g a a t a a g g g a a a a a a c g c c g t a a a a c

1500 20 signal sequence LPVFA 25 30 35 HPETLVKVKDAEDQL AmpR LPVFAHPETLVKVKDAEDQL c c t t c c t g t t t t t g c t c a c c c a g a a a c g c t g g t g a a a g t a a a a g a t g c t g a a g a t c a g t t g g a a g g a c a a a a a c g a g t g g g t c t t t g c g a c c a c t t t c a t t t t c t a c g a c t t c t a g t c a a

1560 40 45 50 55 GARVGYIELDLNSGKILESF AmpR GARVGYIELDLNSGKILESF g g g t g c a c g a g t g g g t t a c a t c g a a c t g g a t c t c a a c a g c g g t a a g a t c c t t g a g a g t t t c c c a c g t g c t c a c c c a a t g t a g c t t g a c c t a g a g t t g t c g c c a t t c t a g g a a c t c t c a a a

1620 60 65 70 75 RPEERFPMMSTFKVLLCGAV AmpR C G A T A C A C C G C G C C A Amp-R RPEERFPMMSTFKVLLCGAV t c g c c c c g a a g a a c g t t t t c c a a t g a t g a g c a c t t t t a a a g t t c t g c t a t g t g g c g c g g t a g c g g g g c t t c t t g c a a a a g g t t a c t a c t c g t g a a a a t t t c a a g a c g a t a c a c c g c g c c a

BsaHI 1680 80 85 90 95 LSRIDAGQEQLGRRIHYSQN AmpR T A A T A Amp-R LSRIDAGQEQLGRRIHYSQN a t t a t c c c g t a t t g a c g c c g g g c a a g a g c a a c t c g g t c g c c g c a t a c a c t a t t c t c a g a a t a a t a g g g c a t a a c t g c g g c c c g t t c t c g t t g a g c c a g c g g c g t a t g t g a t a a g a g t c t t

ScaI 1740 100 105 110 115 DLVEYSPVTEKHLTDGMTVR AmpR DLVEYSPVTEKHLTDGMTVR t g a c t t g g t t g a g t a c t c a c c a g t c a c a g a a a a g c a t c t t a c g g a t g g c a t g a c a g t a a g a c t g a a c c a a c t c a t g a g t g g t c a g t g t c t t t t c g t a g a a t g c c t a c c g t a c t g t c a t t c

1800 120 125 130 135 ELCSAAITMSDNTAANLLLT AmpR ELCSAAITMSDNTAANLLLT a g a a t t a t g c a g t g c t g c c a t a a c c a t g a g t g a t a a c a c t g c g g c c a a c t t a c t t c t g a c t c t t a a t a c g t c a c g a c g g t a t t g g t a c t c a c t a t t g t g a c g c c g g t t g a a t g a a g a c t g

1860 140 145 150 155 TIGGPKELTAFLHNMGDHVT AmpR TIGGPKELTAFLHNMGDHVT a a c g a t c g g a g g a c c g a a g g a g c t a a c c g c t t t t t t g c a c a a c a t g g g g g a t c a t g t a a c t t g c t a g c c t c c t g g c t t c c t c g a t t g g c g a a a a a a c g t g t t g t a c c c c c t a g t a c a t t g

1920 160 165 170 175 RLDRWEPELNEAIPNDERDT AmpR RLDRWEPELNEAIPNDERDT t c g c c t t g a t c g t t g g g a a c c g g a g c t g a a t g a a g c c a t a c c a a a c g a c g a g c g t g a c a c a g c g g a a c t a g c a a c c c t t g g c c t c g a c t t a c t t c g g t a t g g t t t g c t g c t c g c a c t g t g

FspIPstI 1980 180 185 190 195 TMPAAMATTLRKLLTGELLT AmpR TMPAAMATTLRKLLTGELLT c a c g a t g c c t g c a g c a a t g g c a a c a a c g t t g c g c a a a c t a t t a a c t g g c g a a c t a c t t a c g t g c t a c g g a c g t c g t t a c c g t t g t t g c a a c g c g t t t g a t a a t t g a c c g c t t g a t g a a t g

NmeAIIIAseI 2040 200 205 210 215 LASRQQLIDWMEADKVAGPL AmpR LASRQQLIDWMEADKVAGPL t c t a g c t t c c c g g c a a c a a t t a a t a g a c t g g a t g g a g g c g g a t a a a g t t g c a g g a c c a c t a g a t c g a a g g g c c g t t g t t a a t t a t c t g a c c t a c c t c c g c c t a t t t c a a c g t c c t g g t g a

BsrFIBglI 2100 220 225 230 235 LRSALPAGWFIADKSGAGER AmpR LRSALPAGWFIADKSGAGER t c t g c g c t c g g c c c t t c c g g c t g g c t g g t t t a t t g c t g a t a a a t c t g g a g c c g g t g a g c g a g a c g c g a g c c g g g a a g g c c g a c c g a c c a a a t a a c g a c t a t t t a g a c c t c g g c c a c t c g c

BsaI 2160 240 245 250 255 GSRGIIAALGPDGKPSRIVV AmpR GSRGIIAALGPDGKPSRIVV t g g g t c t c g c g g t a t c a t t g c a g c a c t g g g g c c a g a t g g t a a g c c c t c c c g t a t c g t a g t a c c c a g a g c g c c a t a g t a a c g t c g t g a c c c c g g t c t a c c a t t c g g g a g g g c a t a g c a t c a

AhdI 2220 260 265 270 275 IYTTGSQATMDERNRQIAEI AmpR IYTTGSQATMDERNRQIAEI t a t c t a c a c g a c g g g g a g t c a g g c a a c t a t g g a t g a a c g a a a t a g a c a g a t c g c t g a g a t a t a g a t g t g c t g c c c c t c a g t c c g t t g a t a c c t a c t t g c t t t a t c t g t c t a g c g a c t c t a

2280 280 285 GASLIKHW* AmpR GASLIKHW* a g g t g c c t c a c t g a t t a a g c a t t g g t a a c t g t c a g a c c a a g t t t a c t c a t a t a t a c t t t a t c c a c g g a g t g a c t a a t t c g t a a c c a t t g a c a g t c t g g t t c a a a t g a g t a t a t a t g a a a t

2340 g a t t g a t t t a a a a c t t c a t t t t t a a t t t a a a a g g a t c t a g g t g a a g a t c c t t t t t g a t a a c t a a c t a a a t t t t g a a g t a a a a a t t a a a t t t t c c t a g a t c c a c t t c t a g g a a a a a c t a t t

2400 t c t c a t g a c c a a a a t c c c t t a a c g t g a g t t t t c g t t c c a c t g a g c g t c a g a c c c c g t a g a a g a g t a c t g g t t t t a g g g a a t t g c a c t c a a a a g c a a g g t g a c t c g c a g t c t g g g g c a t c t

2460 ori a a a g a t c a a a g g a t c t t c t t g a g a t c c t t t t t t t c t g c g c g t a a t c t g c t g c t t g c a a a c t t t c t a g t t t c c t a g a a g a a c t c t a g g a a a a a a a g a c g c g c a t t a g a c g a c g a a c g t t t g

2520 ori a a a a a a a c c a c c g c t a c c a g c g g t g g t t t g t t t g c c g g a t c a a g a g c t a c c a a c t c t t t t t t t t t t t g g t g g c g a t g g t c g c c a c c a a a c a a a c g g c c t a g t t c t c g a t g g t t g a g a a a a

2580 ori t c c g a a g g t a a c t g g c t t c a g c a g a g c g c a g a t a c c a a a t a c t g t c c t t c t a g t g t a g c c a g g c t t c c a t t g a c c g a a g t c g t c t c g c g t c t a t g g t t t a t g a c a g g a a g a t c a c a t c g g

2640 ori g t a g t t a g g c c a c c a c t t c a a g a a c t c t g t a g c a c c g c c t a c a t a c c t c g c t c t g c t a a t c a t c a a t c c g g t g g t g a a g t t c t t g a g a c a t c g t g g c g g a t g t a t g g a g c g a g a c g a t t a

AlwNI 2700 ori c c t g t t a c c a g t g g c t g c t g c c a g t g g c g a t a a g t c g t g t c t t a c c g g g t t g g a c t c a a g g g a c a a t g g t c a c c g a c g a c g g t c a c c g c t a t t c a g c a c a g a a t g g c c c a a c c t g a g t t c

BseYI 2760 ori a c g a t a g t t a c c g g a t a a g g c g c a g c g g t c g g g c t g a a c g g g g g g t t c g t g c a c a c a g c c t g c t a t c a a t g g c c t a t t c c g c g t c g c c a g c c c g a c t t g c c c c c c a a g c a c g t g t g t c g g

PspFI 2820 ori c a g c t t g g a g c g a a c g a c c t a c a c c g a a c t g a g a t a c c t a c a g c g t g a g c t a t g a g a a a g g t c g a a c c t c g c t t g c t g g a t g t g g c t t g a c t c t a t g g a t g t c g c a c t c g a t a c t c t t t c

2880 ori c g c c a c g c t t c c c g a a g g g a g a a a g g c g g a c a g g t a t c c g g t a a g c g g c a g g g t c g g a a c g c g g t g c g a a g g g c t t c c c t c t t t c c g c c t g t c c a t a g g c c a t t c g c c g t c c c a g c c t t g

2940 ori G G G A A A C G C C T G G T A T C T T T pBR322ori-F a g g a g a g c g c a c g a g g g a g c t t c c a g g g g g a a a c g c c t g g t a t c t t t a t a g t c c t g t c g g t c c t c t c g c g t g c t c c c t c g a a g g t c c c c c t t t g c g g a c c a t a g a a a t a t c a g g a c a g c c

3000 ori g t t t c g c c a c c t c t g a c t t g a g c g t c g a t t t t t g t g a t g c t c g t c a g g g g g g c g g a g c c t c a a a g c g g t g g a g a c t g a a c t c g c a g c t a a a a a c a c t a c g a g c a g t c c c c c c g c c t c g g a

3060 ori a t g g a a a a a c g c c a g c a a c g c g g c c t t t t t a c g g t t c c t g g c c t t t t g c t g g c c t t t t g c t a c c t t t t t g c g g t c g t t g c g c c g g a a a a a t g c c a a g g a c c g g a a a a c g a c c g g a a a a c g

AflIIIPciI 3120 t c a c a t g t t c t t t c c t g c g t t a t c c c c t g a t t c t g t g g a t a a c c g t a t t a c c g c c t t t g a a g t g t a c a a g a a a g g a c g c a a t a g g g g a c t a a g a c a c c t a t t g g c a t a a t g g c g g a a a c t

3180 A G C G A G T C A G T G A G C G A G L4440 g t g a g c t g a t a c c g c t c g c c g c a g c c g a a c g a c c g a g c g c a g c g a g t c a g t g a g c g a g g a c a c t c g a c t a t g g c g a g c g g c g t c g g c t t g c t g g c t c g c g t c g c t c a g t c a c t c g c t c c t

BspQISapI 3240 bom a g c g g a a g a g c g c c t g a t g c g g t a t t t t c t c c t t a c g c a t c t g t g c g g t a t t t c a c a c c g t c g c c t t c t c g c g g a c t a c g c c a t a a a a g a g g a a t g c g t a g a c a c g c c a t a a a g t g t g g c

AccIBstZ17I 3300 bom A T G T T A G A C G A G A C T A C G G C pRS-marker c a a t g g t g c a c t c t c a g t a c a a t c t g c t c t g a t g c c g c a t a g t t a a g c c a g t a t a c a c t c g t t a c c a c g t g a g a g t c a t g t t a g a c g a g a c t a c g g c g t a t c a a t t c g g t c a t a t g t g a g

BsaAI 3360 bom c g c t a t c g c t a c g t g a c t g g g t c a t g g c t g c g c c c c g a c a c c c g c c a a c a c c c g c t g a c g g c g a t a g c g a t g c a c t g a c c c a g t a c c g a c g c g g g g c t g t g g g c g g t t g t g g g c g a c t g c

PfoI 3420 C C G pGEX 3' c g c c c t g a c g g g c t t g t c t g c t c c c g g c a t c c g c t t a c a g a c a a g c t g t g a c c g t c t c c g g c g g g a c t g c c c g a a c a g a c g a g g g c c g t a g g c g a a t g t c t g t t c g a c a c t g g c a g a g g c

PvuII 3480 60 55 50 *LNEGDDGFRALCSRY rop G G A G C T G C A T G T G T C A G A G G pGEX 3' g g a g c t g c a t g t g t c a g a g g t t t t c a c c g t c a t c a c c g a a a c g c g c g a g g c a g c t g c g g t c c t c g a c g t a c a c a g t c t c c a a a a g t g g c a g t a g t g g c t t t g c g c g c t c c g t c g a c g c c a

3540 45 40 35 30 LEDAHDHLSECIDAQEDADL rop a a a g c t c a t c a g c g t g g t c g t g a a g c g a t t c a c a g a t g t c t g c c t g t t c a t c c g c g t c c a t t t c g a g t a g t c g c a c c a g c a c t t c g c t a a g t g t c t a c a g a c g g a c a a g t a g g c g c a g g t

3600 25 20 15 10 ENLKELLTLTQSRIFRAMNL rop g c t c g t t g a g t t t c t c c a g a a g c g t t a a t g t c t g g c t t c t g a t a a a g c g g g c c a t g t t a a c g a g c a a c t c a a a g a g g t c t t c g c a a t t a c a g a c c g a a g a c t a t t t c g c c c g g t a c a a t t

3660 5 1 ATKEQKTM rop g g g c g g t t t t t t c c t g t t t g g t c a c t g a t g c c t c c g t g t a a g g g g g a t t t c t g t t c a t g g c c c g c c a a a a a a g g a c a a a c c a g t g a c t a c g g a g g c a c a t t c c c c c t a a a g a c a a g t a c c

3720 g g g t a a t g a t a c c g a t g a a a c g a g a g a g g a t g c t c a c g a t a c g g g t t a c t g a t g a t g a a c c c c a t t a c t a t g g c t a c t t t g c t c t c t c c t a c g a g t g c t a t g c c c a a t g a c t a c t a c t t g

3780 a t g c c c g g t t a c t g g a a c g t t g t g a g g g t a a a c a a c t g g c g g t a t g g a t g c g g c g g g a c c t a c g g g c c a a t g a c c t t g c a a c a c t c c c a t t t g t t g a c c g c c a t a c c t a c g c c g c c c t g g

AfeI 3840 a g a g a a a a a t c a c t c a g g g t c a a t g c c a g c g c t t c g t t a a t a c a g a t g t a g g t g t t c c a c t c t c t t t t t a g t g a g t c c c a g t t a c g g t c g c g a a g c a a t t a t g t c t a c a t c c a c a a g g t g

BsaBI*BspEI* 3900 a g g g t a g c c a g c a g c a t c c t g c g a t g c a g a t c c g g a a c a t a a t g g t g c a g g g c g c t g a c t t c c c a t c g g t c g t c g t a g g a c g c t a c g t c t a g g c c t t g t a t t a c c a c g t c c c g c g a c t g a

BbsIBsgI* 3960 t c c g c g t t t c c a g a c t t t a c g a a a c a c g g a a a c c g a a g a c c a t t c a t g t t g t t g c t c a g g a g g c g c a a a g g t c t g a a a t g c t t t g t g c c t t t g g c t t c t g g t a a g t a c a a c a a c g a g t c c

4020 t c g c a g a c g t t t t g c a g c a g c a g t c g c t t c a c g t t c g c t c g c g t a t c g g t g a t t c a t t c t a g c g t c t g c a a a a c g t c g t c g t c a g c g a a g t g c a a g c g a g c g c a t a g c c a c t a a g t a a g a

4027 g c t a a c c c g a t t g g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.