Addgene pCDNA3 Flag MKK7a1 Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pCDNA3 Flag MKK7a1 Sequencing Result

Map View

600 400 200 End (758) BglI (749) DraIII (728) HincII (707) EaeI (695) MmeI (664) BsrBI (659) NruI (625) ApaI (537) PspOMI (533) BsmI (532) BsgI (526) NspI (505) PciI - AflIII (501) SacI - BsiHKAI (493) XcmI Eco53kI (491) BtgI - StyI - NcoI (484) AatII (475) ZraI (473) SmlI (411) ScaI (409) BpuEI (396) EarI (367) HaeII (359) BsrDI (334) BbsI (327) BspEI - BsaWI (316) BspHI * (196) PstI (190) AlwNI (185) PspFI (126) BseYI (122) HindIII (75) BsaI (62) T7 (49 .. 68) AseI (48) Start (0) T7 promoter FLAG pCDNA3 Flag MKK7a1 758 bp

Sequence View

AseIStart (0) 5ʹ 3ʹ 60 T7 promoter T A A T A C G A C T C A T7 a g c t c t c t g g c t a a c t a g a g a a c c c a c t g c t t a c t g g c t t a t c g a a a t t a a t a c g a c t c a t c g a g a g a c c g a t t g a t c t c t t g g g t g a c g a a t g a c c g a a t a g c t t t a a t t a t g c t g a g t

HindIIIBsaI 120 1 5 DYKDDDDK T7 promoter FLAG C T A T A G G G T7 MDYKDDDDK c t a t a g g g a g a c c c a a g c t t c g g c a g g c c a a c a t g g a c t a t a a g g a c g a t g a t g a c a a a a g a t a t c c c t c t g g g t t c g a a g c c g t c c g g t t g t a c c t g a t a t t c c t g c t a c t a c t g t t t t

BseYIPspFI 180 (in frame with FLAG) MLGLPSTLFTPRSMESIEID MLGLPSTLFTPRSMESIEID *GQECRATHLADLNV t g c t g g g g c t c c c a t c a a c c t t g t t c a c a c c g c g c a g t a t g g a g a g c a t c g a g a t t g a c c a c g a c c c c g a g g g t a g t t g g a a c a a g t g t g g c g c g t c a t a c c t c t c g t a g c t c t a a c t g g

BspHI*PstIAlwNI 240 (in frame with FLAG) QKLQEIMKQTGYLTIGGQRY QKLQEIMKQTGYLTIGGQRY LLQLLDHLLCPVQSDPALTI a g a a g c t g c a g g a g a t c a t g a a g c a g a c a g g g t a c c t g a c t a t c g g g g g c c a g c g t t a t c t c t t c g a c g t c c t c t a g t a c t t c g t c t g t c c c a t g g a c t g a t a g c c c c c g g t c g c a a t a g

300 (in frame with FLAG) QAEINDLENLGEMGSGTCGQ QAEINDLENLGEMGSGTCGQ LCFDIVQLVQTLHATTGTTL a g g c a g a a a t c a a t g a c t t g g a g a a c t t g g g t g a g a t g g g c a g t g g t a c c t g t g g t c a g g t c c g t c t t t a g t t a c t g a a c c t c t t g a a c c c a c t c t a c c c g t c a c c a t g g a c a c c a g t c c

HaeIIBsrDIBbsIBsaWIBspEI 360 (in frame with FLAG) VWKMRFRKTGHIIAVKQMRR VWKMRFRKTGHIIAVKQMRR HPLHPEPLCAVDNSNLLHPA t g t g g a a g a t g c g g t t c c g g a a g a c a g g c c a c a t c a t t g c t g t t a a g c a a a t g c g g c g c t a c a c c t t c t a c g c c a a g g c c t t c t g t c c g g t g t a g t a a c g a c a a t t c g t t t a c g c c g c g a

ScaISmlIBpuEIEarI 420 (in frame with FLAG) SGNKEENKRILMDLDVVLKS SGNKEENKRILMDLDVVLKS RPVLFLILANQHVQIYYELA c t g g g a a c a a g g a a g a g a a t a a g c g c a t t t t g a t g g a c c t g g a t g t a g t a c t c a a g a g c c g a c c c t t g t t c c t t c t c t t a t t c g c g t a a a a c t a c c t g g a c c t a c a t c a t g a g t t c t c g g

ZraIAatII 480 (in frame with FLAG) HDCPYIVQCFGTFITNTDVF HDCPYIVQCFGTFITNTDVF MVARVDNLAKAGEDGVCVDK a t g a c t g c c c t t a c a t c g t t c a g t g c t t t g g c a c c t t c a t c a c c a a c a c a g a c g t c t t t a t a c t g a c g g g a a t g t a g c a a g t c a c g a a a c c g t g g a a g t a g t g g t t g t g t c t g c a g a a a t

BsgIBsmIPspOMIApaINcoIStyIBtgIEco53kIXcmIBsiHKAISacIAflIIIPciINspI 540 (in frame with FLAG) IAMELMGTCAEKLKKRMQGP IAMELMGTCAEKLKKRMQGP NGHLEHACTCLLQLFSHLAG t t g c c a t g g a g c t c a t g g g c a c a t g t g c a g a g a a g c t g a a g a a a c g a a t g c a g g g c c c c a a a c g g t a c c t c g a g t a c c c g t g t a c a c g t c t c t t c g a c t t c t t t g c t t a c g t c c c g g g g t

600 (in frame with FLAG) IPERILGKMTVAIVKALYYL IPERILGKMTVAIVKALYYL NWLSDQALHSHRNHFCQVIQ t t c c a g a g c g a a t c c t g g g c a a g a t g a c t g t g g c g a t t g t g a a a g c a c t g t a c t a t c t g a a a g g t c t c g c t t a g g a c c c g t t c t a c t g a c a c c g c t a a c a c t t t c g t g a c a t g a t a g a c t

BsrBINruI 660 (in frame with FLAG) KEKHGVIHRDVKPSNILLDE KEKHGVIHRDVKPSNILLDE LLLMADDM a g g a g a a g c a t g g c g t c a t c c a t c g c g a t g t c a a a c c c t c c a a c a t c c t g c t a g a t g a g c t c c t c t t c g t a c c g c a g t a g g t a g c g c t a c a g t t t g g g a g g t t g t a g g a c g a t c t a c t c g

HincIIEaeIMmeI 720 (in frame with FLAG) RGQIKLCDFGISGRLVDSKP RGQIKLCDFGISGRLVDSKP g g g g c c a g a t c a a g c t c t g t g a c t t t g g c a t c a g t g g c c g c c t t g t t g a c t c c a a g c c a a c c c c g g t c t a g t t c g a g a c a c t g a a a c c g t a g t c a c c g g c g g a a c a a c t g a g g t t c g g t t

End (758) BglIDraIII 3ʹ 5ʹ 758 (in frame with FLAG) KHGVLAVLPIWLP KHGVLAVLPIWLP a a c a c g g a g t g c t g g c t g t g c t g c c t a t a t g g c t c c c g t t g t g c c t c a c g a c c g a c a c g a c g g a t a t a c c g a g g g c

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.