Addgene mEos3.2-MCPB1-C-18 Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: mEos3.2-MCPB1-C-18 Sequencing Result


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PciI (5483) pBR322ori-F (5328 .. 5347) ApaLI (5169) EcoO109I (4663) TK-pA-R (4419 .. 4438) PfoI (4340) Neo-F (4101 .. 4120) RsrII (4081) BsrDI (3798) FspI (3667) MscI (3647) Neo-R (3491 .. 3510) BspDI * - ClaI * (3405) StuI (3386) BseRI (3383) SfiI (3340) pBABE 3' (3068 .. 3088) F1ori-R (2806 .. 2825) AseI (0) NdeI (227) SnaBI (333) CMV-F (512 .. 532) NheI (584) BmtI (588) AfeI (589) AgeI (593) EcoRV (770) HindIII (878) BspEI * (1284) PaeR7I - XhoI (1297) BbvCI (1407) BstEII (1549) PmlI (1790) BsgI (1821) EcoNI (1983) EcoRI (2169) PstI (2178) SalI (2179) SacII (2192) PspOMI (2193) TspMI - XmaI (2196) ApaI (2197) SmaI (2198) XbaI * (2212) MfeI (2315) HpaI (2328) SV40pA-R (2366 .. 2385) EBV-rev (2420 .. 2439) MluI (2451) F1ori-F (2594 .. 2615) mEos3.2-MCPB1-C-18 5548 bp

Sequence View

AseI 5ʹ 3ʹ 60 CMV enhancer t a a t a g t a a t c a a t t a c g g g g t c a t t a g t t c a t a g c c c a t a t a t g g a g t t c c g c g t t a c a a t t a t c a t t a g t t a a t g c c c c a g t a a t c a a g t a t c g g g t a t a t a c c t c a a g g c g c a a t g t

120 CMV enhancer t a a c t t a c g g t a a a t g g c c c g c c t g g c t g a c c g c c c a a c g a c c c c c g c c c a t t g a c g t c a a t t g a a t g c c a t t t a c c g g g c g g a c c g a c t g g c g g g t t g c t g g g g g c g g g t a a c t g c a g t

180 CMV enhancer a t a a t g a c g t a t g t t c c c a t a g t a a c g c c a a t a g g g a c t t t c c a t t g a c g t c a a t g g g t g t a t t a c t g c a t a c a a g g g t a t c a t t g c g g t t a t c c c t g a a a g g t a a c t g c a g t t a c c c a c

NdeI 240 CMV enhancer g a g t a t t t a c g g t a a a c t g c c c a c t t g g c a g t a c a t c a a g t g t a t c a t a t g c c a a g t a c g c t c a t a a a t g c c a t t t g a c g g g t g a a c c g t c a t g t a g t t c a c a t a g t a t a c g g t t c a t g c

300 CMV enhancer c c c c c t a t t g a c g t c a a t g a c g g t a a a t g g c c c g c c t g g c a t t a t g c c c a g t a c a t g a c c g g g g g a t a a c t g c a g t t a c t g c c a t t t a c c g g g c g g a c c g t a a t a c g g g t c a t g t a c t g g

SnaBI 360 CMV enhancer CMV promoter t t a t g g g a c t t t c c t a c t t g g c a g t a c a t c t a c g t a t t a g t c a t c g c t a t t a c c a t g g t g a a t a c c c t g a a a g g a t g a a c c g t c a t g t a g a t g c a t a a t c a g t a g c g a t a a t g g t a c c a c

420 CMV promoter a t g c g g t t t t g g c a g t a c a t c a a t g g g c g t g g a t a g c g g t t t g a c t c a c g g g g a t t t c c a t a c g c c a a a a c c g t c a t g t a g t t a c c c g c a c c t a t c g c c a a a c t g a g t g c c c c t a a a g g t

480 CMV promoter a g t c t c c a c c c c a t t g a c g t c a a t g g g a g t t t g t t t t g g c a c c a a a a t c a a c g g g a c t t t t c a g a g g t g g g g t a a c t g c a g t t a c c c t c a a a c a a a a c c g t g g t t t t a g t t g c c c t g a a a

540 CMV promoter C G C A A A T G G G C G G T A G G C G T G CMV-F c c a a a a t g t c g t a a c a a c t c c g c c c c a t t g a c g c a a a t g g g c g g t a g g c g t g t a c g g t g g g g t t t t a c a g c a t t g t t g a g g c g g g g t a a c t g c g t t t a c c c g c c a t c c g c a c a t g c c a c c

AgeINheIBmtIAfeI 600 CMV promoter *RD g a g g t c t a t a t a a g c a g a g c t g g t t t a g t g a a c c g t c a g a t c c g c t a g c g c t a c c g g t c g c t c c a g a t a t a t t c g t c t c g a c c a a a t c a c t t g g c a g t c t a g g c g a t c g c g a t g g c c a g c

660 1 5 10 15 MSAIKPDMKIKLRMEGNV mEos3.2 MSAIKPDMKIKLRMEGNV GGHTRNLWVHLDFETHFAVY c c a c c a t g a g t g c g a t t a a g c c a g a c a t g a a g a t c a a a c t c c g t a t g g a a g g c a a c g t a a g g t g g t a c t c a c g c t a a t t c g g t c t g t a c t t c t a g t t t g a g g c a t a c c t t c c g t t g c a t t

720 20 25 30 35 NGHHFVIDGDGTGKPFEGKQ mEos3.2 NGHHFVIDGDGTGKPFEGKQ VPVVKHDVSITCALRKLSFL a c g g g c a c c a c t t t g t g a t c g a c g g a g a t g g t a c a g g c a a g c c t t t t g a g g g a a a a c a g a t g c c c g t g g t g a a a c a c t a g c t g c c t c t a c c a t g t c c g t t c g g a a a a c t c c c t t t t g t c t

EcoRV 780 40 45 50 55 SMDLEVKEGGPLPFAFDILT mEos3.2 SMDLEVKEGGPLPFAFDILT THIKFDFLASRQRKGKIDQG g t a t g g a t c t t g a a g t c a a a g a g g g c g g a c c t c t g c c t t t t g c c t t t g a t a t c c t g a c c a c a t a c c t a g a a c t t c a g t t t c t c c c g c c t g g a g a c g g a a a a c g g a a a c t a t a g g a c t g g t

840 60 65 70 75 TAFHYGNRVFAKYPDNIQDY mEos3.2 TAFHYGNRVFAKYPDNIQDY SCEMVAVPYEGFIWVVYLVI c t g c a t t c c a t t a c g g c a a c a g g g t a t t c g c c a a a t a t c c a g a c a a c a t a c a a g a c t a t t g a c g t a a g g t a a t g c c g t t g t c c c a t a a g c g g t t t a t a g g t c t g t t g t a t g t t c t g a t a a

HindIII 900 80 85 90 95 FKQSFPKGYSWERSLTFEDG mEos3.2 FKQSFPKGYSWERSLTFEDG KLLRKRLPIRPFSAQSEFVP t t a a g c a g t c g t t t c c t a a g g g g t a t t c g t g g g a a c g a a g c t t g a c t t t c g a a g a c g g g g a a t t c g t c a g c a a a g g a t t c c c c a t a a g c a c c c t t g c t t c g a a c t g a a a g c t t c t g c c c c

960 100 105 110 115 GICNARNDITMEGDTFYNKV mEos3.2 GICNARNDITMEGDTFYNKV ANAVGSVVYCHFPVSEIIFN g c a t t t g c a a c g c c a g a a a c g a c a t a a c a a t g g a a g g g g a c a c t t t c t a t a a t a a a g t t c c g t a a a c g t t g c g g t c t t t g c t g t a t t g t t a c c t t c c c c t g t g a a a g a t a t t a t t t c a a g

1020 120 125 130 135 RFYGTNFPANGPVMQKKTLK mEos3.2 RFYGTNFPANGPVMQKKTLK SKITGVKGGITWNHLLLRQF g a t t t t a t g g t a c c a a c t t t c c c g c c a a t g g t c c a g t t a t g c a g a a g a a g a c g c t g a a a t c t a a a a t a c c a t g g t t g a a a g g g c g g t t a c c a g g t c a a t a c g t c t t c t t c t g c g a c t t t a

1080 140 145 150 155 WEPSTEKMYVRDGVLTGDIE mEos3.2 WEPSTEKMYVRDGVLTGDIE PLGGSLFHIHTISHQRTINL g g g a g c c c t c c a c t g a g a a a a t g t a t g t g c g t g a t g g a g t g c t g a c g g g t g a t a t t g a g a c c c t c g g g a g g t g a c t c t t t t a c a t a c a c g c a c t a c c t c a c g a c t g c c c a c t a t a a c t c t

1140 160 165 170 175 MALLLEGNAHYRCDFRTTYK mEos3.2 MALLLEGNAHYRCDFRTTYK HSQQKFSIGM t g g c t t t g t t g c t t g a a g g a a a t g c c c a t t a c c g a t g t g a c t t c a g a a c t a c t t a c a a a g a c c g a a a c a a c g a a c t t c c t t t a c g g g t a a t g g c t a c a c t g a a g t c t t g a t g a a t g t t t c

1200 180 185 190 195 AKEKGVKLPGAHFVDHCIEI mEos3.2 AKEKGVKLPGAHFVDHCIEI c t a a g g a g a a g g g t g t c a a g t t a c c a g g c g c c c a c t t t g t g g a c c a c t g c a t t g a g a t t t g a t t c c t c t t c c c a c a g t t c a a t g g t c c g c g g g t g a a a c a c c t g g t g a c g t a a c t c t a a a

1260 200 205 210 215 LSHDKDYNKVKLYEHAVAHS mEos3.2 LSHDKDYNKVKLYEHAVAHS t a a g c c a t g a c a a a g a t t a c a a c a a g g t t a a g c t g t a t g a g c a t g c t g t t g c t c a t t c t g a t t c g g t a c t g t t t c t a a t g t t g t t c c a a t t c g a c a t a c t c g t a c g a c a a c g a g t a a g a c

PaeR7IXhoIBspEI* 1320 220 225 GLPDNARR (in frame with mEos3.2) SGLRSRESSGYS mEos3.2 GLPDNARRSGLRSRESSGYS g a t t g c c t g a c a a t g c c a g a c g a t c c g g a c t c a g a t c t c g a g a g t c c a g t g g a t a t t c g t c t a a c g g a c t g t t a c g g t c t g c t a g g c c t g a g t c t a g a g c t c t c a g g t c a c c t a t a a g c a

1380 (in frame with mEos3.2) SRSGGSSDQQLDCALDLMRR SRSGGSSDQQLDCALDLMRR c t c g g t c g g g t g g a a g c a g c g a t c a g c a g c t g g a c t g c g c c t t g g a c c t g a t g a g g c g c c g a g c c a g c c c a c c t t c g t c g c t a g t c g t c g a c c t g a c g c g g a a c c t g g a c t a c t c c g c g g

BbvCI 1440 (in frame with mEos3.2) LPPQQIEKNLSDLIDLVPSL LPPQQIEKNLSDLIDLVPSL t g c c t c c a c a g c a g a t t g a g a a g a a c c t c a g c g a t c t g a t c g a c c t g g t c c c c a g t c t g t a c g g a g g t g t c g t c t a a c t c t t c t t g g a g t c g c t a g a c t a g c t g g a c c a g g g g t c a g a c a

1500 (in frame with mEos3.2) CEDLLSSVDQPLKIARDKVV CEDLLSSVDQPLKIARDKVV g t g a a g a t c t c c t g t c a t c t g t t g a c c a g c c c c t g a a a a t t g c c a g a g a c a a g g t g g t g g c a c t t c t a g a g g a c a g t a g a c a a c t g g t c g g g g a c t t t t a a c g g t c t c t g t t c c a c c a c c

BstEII 1560 (in frame with mEos3.2) GKDYLLCDYNRDGDSYRSPW GKDYLLCDYNRDGDSYRSPW g c a a g g a t t a c c t t t t g t g t g a c t a c a a c a g a g a c g g g g a c t c c t a t a g g t c a c c g t g g a c g t t c c t a a t g g a a a a c a c a c t g a t g t t g t c t c t g c c c c t g a g g a t a t c c a g t g g c a c c t

1620 (in frame with mEos3.2) SNKYDPPLEDGAMPSARLRK SNKYDPPLEDGAMPSARLRK g t a a c a a g t a t g a c c c t c c t t t g g a a g a t g g g g c c a t g c c a t c t g c t c g g c t c a g a a a g c c a t t g t t c a t a c t g g g a g g a a a c c t t c t a c c c c g g t a c g g t a g a c g a g c c g a g t c t t t c g

1680 (in frame with mEos3.2) LEVEANNAFDQYRDLYFEGG LEVEANNAFDQYRDLYFEGG t g g a g g t a g a g g c c a a c a a t g c c t t c g a c c a a t a c c g a g a c c t g t a t t t t g a a g g t g g g g a c c t c c a t c t c c g g t t g t t a c g g a a g c t g g t t a t g g c t c t g g a c a t a a a a c t t c c a c c c c

1740 (in frame with mEos3.2) VSSVYLWDLDHGFAGVILIK VSSVYLWDLDHGFAGVILIK t c t c a t c a g t c t a c c t c t g g g a t c t t g a t c a t g g c t t t g c t g g a g t g a t c c t c a t a a a g a a g a g t a g t c a g a t g g a g a c c c t a g a a c t a g t a c c g a a a c g a c c t c a c t a g g a g t a t t t c t

PmlI 1800 (in frame with mEos3.2) KAGDGSKKIKGCWDSIHVVE KAGDGSKKIKGCWDSIHVVE a a g c t g g a g a t g g a t c c a a g a a g a t c a a a g g c t g c t g g g a t t c c a t c c a c g t g g t g g a a g t t c g a c c t c t a c c t a g g t t c t t c t a g t t t c c g a c g a c c c t a a g g t a g g t g c a c c a c c t t c

BsgI 1860 (in frame with mEos3.2) VQEKSSGRTAHYKLTSTVML VQEKSSGRTAHYKLTSTVML t g c a g g a g a a g t c c a g c g g c c g t a c t g c c c a t t a c a a g t t g a c c t c c a c g g t g a t g c t a t a c g t c c t c t t c a g g t c g c c g g c a t g a c g g g t a a t g t t c a a c t g g a g g t g c c a c t a c g a t a

1920 (in frame with mEos3.2) WLQTNKSGSGTMNLGGSLTR WLQTNKSGSGTMNLGGSLTR g g c t g c a a a c c a a c a a a t c c g g c t c g g g c a c c a t g a a c c t g g g a g g c a g c c t a a c c a g a c c c g a c g t t t g g t t g t t t a g g c c g a g c c c g t g g t a c t t g g a c c c t c c g t c g g a t t g g t c t g

1980 (in frame with mEos3.2) QMEKDETVSDCSPHIANIGR QMEKDETVSDCSPHIANIGR a g a t g g a g a a a g a c g a a a c t g t g a g t g a c t g t t c c c c a c a c a t a g c c a a c a t c g g g c g c c t c t a c c t c t t t c t g c t t t g a c a c t c a c t g a c a a g g g g t g t g t a t c g g t t g t a g c c c g c g g

EcoNI 2040 (in frame with mEos3.2) LVEDMENKIRSTLNEIYFGK LVEDMENKIRSTLNEIYFGK t g g t g g a g g a c a t g g a a a a c a a a a t c c g a a g c a c g c t g a a t g a g a t c t a c t t t g g a a a a a a c c a c c t c c t g t a c c t t t t g t t t t a g g c t t c g t g c g a c t t a c t c t a g a t g a a a c c t t t t t

2100 (in frame with mEos3.2) TKDIVNGLRSLDAIPDNHKF TKDIVNGLRSLDAIPDNHKF c a a a g g a c a t c g t c a a c g g g c t g a g a t c t c t t g a t g c t a t c c c c g a c a a c c a c a a g t t t a g t t t c c t g t a g c a g t t g c c c g a c t c t a g a g a a c t a c g a t a g g g g c t g t t g g t g t t c a a a t

2160 (in frame with mEos3.2) KQLQRELSQVLTQRQVYIQP KQLQRELSQVLTQRQVYIQP a g c a g t t g c a g a g g g a a c t t t c t c a a g t g c t g a c c c a g c g c c a g g t c t a c a t c c a g c c t g t c g t c a a c g t c t c c c t t g a a a g a g t t c a c g a c t g g g t c g c g g t c c a g a t g t a g g t c g g a c

XbaI*SacIIPspOMITspMIXmaIApaISmaIPstISalIEcoRI 2220 DN* DN* a t a a t t g a g a a t t c t g c a g t c g a c g g t a c c g c g g g c c c g g g a t c c a c c g g a t c t a g a t a a t a t t a a c t c t t a a g a c g t c a g c t g c c a t g g c g c c c g g g c c c t a g g t g g c c t a g a t c t a t t

2280 c t g a t c a t a a t c a g c c a t a c c a c a t t t g t a g a g g t t t t a c t t g c t t t a a a a a a c c t c c c a g a c t a g t a t t a g t c g g t a t g g t g t a a a c a t c t c c a a a a t g a a c g a a a t t t t t t g g a g g g t

HpaIMfeI 2340 SV40 poly(A) signal c a c c t c c c c c t g a a c c t g a a a c a t a a a a t g a a t g c a a t t g t t g t t g t t a a c t t g t t t a t t g t g g a g g g g g a c t t g g a c t t t g t a t t t t a c t t a c g t t a a c a a c a a c a a t t g a a c a a a t a a

2400 SV40 poly(A) signal C G T T A T C G T A G T G T T T A A A G SV40pA-R g c a g c t t a t a a t g g t t a c a a a t a a a g c a a t a g c a t c a c a a a t t t c a c a a a t a a a g c a t t t c g t c g a a t a t t a c c a a t g t t t a t t t c g t t a t c g t a g t g t t t a a a g t g t t t a t t t c g t a a a

MluI 2460 SV40 poly(A) signal f1 ori G T G G T T T G T C C A A A C T C A T C EBV-rev t t t t c a c t g c a t t c t a g t t g t g g t t t g t c c a a a c t c a t c a a t g t a t c t t a a c g c g t a a a t a a a a g t g a c g t a a g a t c a a c a c c a a a c a g g t t t g a g t a g t t a c a t a g a a t t g c g c a t t t a

2520 f1 ori t g t a a g c g t t a a t a t t t t g t t a a a a t t c g c g t t a a a t t t t t g t t a a a t c a g c t c a t t t t t a c a t t c g c a a t t a t a a a a c a a t t t t a a g c g c a a t t t a a a a a c a a t t t a g t c g a g t a a a a a

2580 f1 ori t a a c c a a t a g g c c g a a a t c g g c a a a a t c c c t t a t a a a t c a a a a g a a t a g a c c g a g a t a g g a t t g g t t a t c c g g c t t t a g c c g t t t t a g g g a a t a t t t a g t t t t c t t a t c t g g c t c t a t c c

2640 f1 ori G G T C A A A C C T T G T T C T C A G G T G F1ori-F g t t g a g t g t t g t t c c a g t t t g g a a c a a g a g t c c a c t a t t a a a g a a c g t g g a c t c c a a c g t c a a c t c a c a a c a a g g t c a a a c c t t g t t c t c a g g t g a t a a t t t c t t g c a c c t g a g g t t g c a

2700 f1 ori c a a a g g g c g a a a a a c c g t c t a t c a g g g c g a t g g c c c a c t a c g t g a a c c a t c a c c c t a a t c g t t t c c c g c t t t t t g g c a g a t a g t c c c g c t a c c g g g t g a t g c a c t t g g t a g t g g g a t t a g

2760 f1 ori a a g t t t t t t g g g g t c g a g g t g c c g t a a a g c a c t a a a t c g g a a c c c t a a a g g g a g c c c c c g t t c a a a a a a c c c c a g c t c c a c g g c a t t t c g t g a t t t a g c c t t g g g a t t t c c c t c g g g g g c

2820 f1 ori A G G G A A G A A A G C G A A F1ori-R a t t t a g a g c t t g a c g g g g a a a g c c g g c g a a c g t g g c g a g a a a g g a a g g g a a g a a a g c g a a t a a a t c t c g a a c t g c c c c t t t c g g c c g c t t g c a c c g c t c t t t c c t t c c c t t c t t t c g c t t

2880 f1 ori A G G A G F1ori-R a g g a g c g g g c g c t a g g g c g c t g g c a a g t g t a g c g g t c a c g c t g c g c g t a a c c a c c a c a c c t c c t c g c c c g c g a t c c c g c g a c c g t t c a c a t c g c c a g t g c g a c g c g c a t t g g t g g t g t g g

2940 f1 ori AmpR promoter c g c c g c g c t t a a t g c g c c g c t a c a g g g c g c g t c a g g t g g c a c t t t t c g g g g a a a t g t g c g g c g g c g c g a a t t a c g c g g c g a t g t c c c g c g c a g t c c a c c g t g a a a a g c c c c t t t a c a c g c

3000 AmpR promoter c g g a a c c c c t a t t t g t t t a t t t t t c t a a a t a c a t t c a a a t a t g t a t c c g c t c a t g a g a c a g c c t t g g g g a t a a a c a a a t a a a a a g a t t t a t g t a a g t t t a t a c a t a g g c g a g t a c t c t g t

3060 AmpR promoter SV40 promoter SV40 enhancer a t a a c c c t g a t a a a t g c t t c a a t a a t a t t g a a a a a g g a a g a g t c c t g a g g c g g a a a g a a c t a t t g g g a c t a t t t a c g a a g t t a t t a t a a c t t t t t c c t t c t c a g g a c t c c g c c t t t c t t g

3120 SV40 promoter SV40 enhancer C C T T A C A C A C A G T C A A T C C C A pBABE 3' c a g c t g t g g a a t g t g t g t c a g t t a g g g t g t g g a a a g t c c c c a g g c t c c c c a g c a g g c a g a g t c g a c a c c t t a c a c a c a g t c a a t c c c a c a c c t t t c a g g g g t c c g a g g g g t c g t c c g t c t

3180 SV40 promoter SV40 enhancer a g t a t g c a a a g c a t g c a t c t c a a t t a g t c a g c a a c c a g g t g t g g a a a g t c c c c a g g c t c c t c a t a c g t t t c g t a c g t a g a g t t a a t c a g t c g t t g g t c c a c a c c t t t c a g g g g t c c g a g g

3240 SV40 promoter SV40 enhancer c c a g c a g g c a g a a g t a t g c a a a g c a t g c a t c t c a a t t a g t c a g c a a c c a t a g t c c c g c c c g g t c g t c c g t c t t c a t a c g t t t c g t a c g t a g a g t t a a t c a g t c g t t g g t a t c a g g g c g g g

3300 SV40 promoter SV40 ori c t a a c t c c g c c c a t c c c g c c c c t a a c t c c g c c c a g t t c c g c c c a t t c t c c g c c c c a t g g c g a t t g a g g c g g g t a g g g c g g g g a t t g a g g c g g g t c a a g g c g g g t a a g a g g c g g g g t a c c g

SfiI 3360 SV40 promoter SV40 ori t g a c t a a t t t t t t t t a t t t a t g c a g a g g c c g a g g c c g c c t c g g c c t c t g a g c t a t t c c a g a c t g a t t a a a a a a a a t a a a t a c g t c t c c g g c t c c g g c g g a g c c g g a g a c t c g a t a a g g t c

BspDI*ClaI*BseRIStuI 3420 SV40 promoter SV40 ori a a g t a g t g a g g a g g c t t t t t t g g a g g c c t a g g c t t t t g c a a a g a t c g a t c a a g a g a c a g g t t c a t c a c t c c t c c g a a a a a a c c t c c g g a t c c g a a a a c g t t t c t a g c t a g t t c t c t g t c c

3480 1 5 10 15 MIEQDGLHAGSPAAW NeoR/KanR 1 5 10 15 MIEQDGLHAGSPAAW NeoR/KanR MIEQDGLHAGSPAAW a t g a g g a t c g t t t c g c a t g a t t g a a c a a g a t g g a t t g c a c g c a g g t t c t c c g g c c g c t t g t a c t c c t a g c a a a g c g t a c t a a c t t g t t c t a c c t a a c g t g c g t c c a a g a g g c c g g c g a a c

3540 20 25 30 35 VERLFGYDWAQQTIGCSDAA NeoR/KanR 20 25 30 35 VERLFGYDWAQQTIGCSDAA NeoR/KanR G A T A A G C C G A T A C T G A C C C G Neo-R VERLFGYDWAQQTIGCSDAA g g t g g a g a g g c t a t t c g g c t a t g a c t g g g c a c a a c a g a c a a t c g g c t g c t c t g a t g c c g c c c a c c t c t c c g a t a a g c c g a t a c t g a c c c g t g t t g t c t g t t a g c c g a c g a g a c t a c g g c g

3600 40 45 50 55 VFRLSAQGRPVLFVKTDLSG NeoR/KanR 40 45 50 55 VFRLSAQGRPVLFVKTDLSG NeoR/KanR VFRLSAQGRPVLFVKTDLSG c g t g t t c c g g c t g t c a g c g c a g g g g c g c c c g g t t c t t t t t g t c a a g a c c g a c c t g t c c g g g c a c a a g g c c g a c a g t c g c g t c c c c g c g g g c c a a g a a a a a c a g t t c t g g c t g g a c a g g c c

MscI 3660 60 65 70 75 ALNELQDEAARLSWLATTGV NeoR/KanR 60 65 70 75 ALNELQDEAARLSWLATTGV NeoR/KanR ALNELQDEAARLSWLATTGV t g c c c t g a a t g a a c t g c a a g a c g a g g c a g c g c g g c t a t c g t g g c t g g c c a c g a c g g g c g t a c g g g a c t t a c t t g a c g t t c t g c t c c g t c g c g c c g a t a g c a c c g a c c g g t g c t g c c c g c a

FspI 3720 80 85 90 95 PCAAVLDVVTEAGRDWLLLG NeoR/KanR 80 85 90 95 PCAAVLDVVTEAGRDWLLLG NeoR/KanR PCAAVLDVVTEAGRDWLLLG t c c t t g c g c a g c t g t g c t c g a c g t t g t c a c t g a a g c g g g a a g g g a c t g g c t g c t a t t g g g a g g a a c g c g t c g a c a c g a g c t g c a a c a g t g a c t t c g c c c t t c c c t g a c c g a c g a t a a c c c

3780 100 105 110 115 EVPGQDLLSSHLAPAEKVSI NeoR/KanR 100 105 110 115 EVPGQDLLSSHLAPAEKVSI NeoR/KanR EVPGQDLLSSHLAPAEKVSI *RVKSRGLFYGD c g a a g t g c c g g g g c a g g a t c t c c t g t c a t c t c a c c t t g c t c c t g c c g a g a a a g t a t c c a t g c t t c a c g g c c c c g t c c t a g a g g a c a g t a g a g t g g a a c g a g g a c g g c t c t t t c a t a g g t a

BsrDI 3840 120 125 130 135 MADAMRRLHTLDPATCPFDH NeoR/KanR 120 125 130 135 MADAMRRLHTLDPATCPFDH NeoR/KanR MADAMRRLHTLDPATCPFDH HSICHPPQMRKIRSGAWEVV c a t g g c t g a t g c a a t g c g g c g g c t g c a t a c g c t t g a t c c g g c t a c c t g c c c a t t c g a c c a g t a c c g a c t a c g t t a c g c c g c c g a c g t a t g c g a a c t a g g c c g a t g g a c g g g t a a g c t g g t

3900 140 145 150 155 QAKHRIERARTRMEAGLVDQ NeoR/KanR 140 145 150 155 QAKHRIERARTRMEAGLVDQ NeoR/KanR QAKHRIERARTRMEAGLVDQ LRFMADLSCTSPHFGTKDIL c c a a g c g a a a c a t c g c a t c g a g c g a g c a c g t a c t c g g a t g g a a g c c g g t c t t g t c g a t c a g g t t c g c t t t g t a g c g t a g c t c g c t c g t g c a t g a g c c t a c c t t c g g c c a g a a c a g c t a g t

3960 160 165 170 175 DDLDEEHQGLAPAELFARLK NeoR/KanR 160 165 170 175 DDLDEEHQGLAPAELFARLK NeoR/KanR DDLDEEHQGLAPAELFARLK IIQVFLMLPERWGFQEGPEL g g a t g a t c t g g a c g a a g a g c a t c a g g g g c t c g c g c c a g c c g a a c t g t t c g c c a g g c t c a a c c t a c t a g a c c t g c t t c t c g t a g t c c c c g a g c g c g g t c g g c t t g a c a a g c g g t c c g a g t t

4020 180 185 190 195 ASMPDGEDLVVTHGDACLPN NeoR/KanR 180 185 190 195 ASMPDGEDLVVTHGDACLPN NeoR/KanR ASMPDGEDLVVTHGDACLPN RAHGVALIEDHGMAIGAQRI g g c g a g c a t g c c c g a c g g c g a g g a t c t c g t c g t g a c c c a t g g c g a t g c c t g c t t g c c g a a c c g c t c g t a c g g g c t g c c g c t c c t a g a g c a g c a c t g g g t a c c g c t a c g g a c g a a c g g c t t

4080 200 205 210 215 IMVENGRFSGFIDCGRLGVA NeoR/KanR 200 205 210 215 IMVENGRFSGFIDCGRLGVA NeoR/KanR IMVENGRFSGFIDCGRLGVA DHHFIAAKRSEDVTAPQTHR t a t c a t g g t g g a a a a t g g c c g c t t t t c t g g a t t c a t c g a c t g t g g c c g g c t g g g t g t g g c a t a g t a c c a c c t t t t a c c g g c g a a a a g a c c t a a g t a g c t g a c a c c g g c c g a c c c a c a c c g

RsrII 4140 220 225 230 235 DRYQDIALATRDIAEELGGE NeoR/KanR 220 225 230 235 DRYQDIALATRDIAEELGGE NeoR/KanR C G T T G G C T A C C C G T G A T A T T Neo-F DRYQDIALATRDIAEELGGE VAILVYRQSGTINSFLKAAF g g a c c g c t a t c a g g a c a t a g c g t t g g c t a c c c g t g a t a t t g c t g a a g a g c t t g g c g g c g a c c t g g c g a t a g t c c t g t a t c g c a a c c g a t g g g c a c t a t a a c g a c t t c t c g a a c c g c c g c t

4200 240 245 250 255 WADRFLVLYGIAAPDSQRIA NeoR/KanR 240 245 250 255 WADRFLVLYGIAAPDSQRIA NeoR/KanR WADRFLVLYGIAAPDSQRIA PSVAEEHKVTDGSGIRLADG a t g g g c t g a c c g c t t c c t c g t g c t t t a c g g t a t c g c c g c t c c c g a t t c g c a g c g c a t c g c t a c c c g a c t g g c g a a g g a g c a c g a a a t g c c a t a g c g g c g a g g g c t a a g c g t c g c g t a g c g

4260 260 265 FYRLLDEFF* NeoR/KanR 260 265 FYRLLDEFF* NeoR/KanR MTD FYRLLDEFF* EIAKKVLEESRSEPTRFSRG c t t c t a t c g c c t t c t t g a c g a g t t c t t c t g a g c g g g a c t c t g g g g t t c g a a a t g a c c g a c g a a g a t a g c g g a a g a a c t g c t c a a g a a g a c t c g c c c t g a g a c c c c a a g c t t t a c t g g c t g

4320 QATPNLPSRDFDSTAAFYER LSAWGAM c a a g c g a c g c c c a a c c t g c c a t c a c g a g a t t t c g a t t c c a c c g c c g c c t t c t a t g a a a g g g t t c g c t g c g g g t t g g a c g g t a g t g c t c t a a a g c t a a g g t g g c g g c g g a a g a t a c t t t c c

PfoI 4380 LGFGIVFRDAGWMILQRGDL t t g g g c t t c g g a a t c g t t t t c c g g g a c g c c g g c t g g a t g a t c c t c c a g c g c g g g g a t c t c a a c c c g a a g c c t t a g c a a a a g g c c c t g c g g c c g a c c t a c t a g g a g g t c g c g c c c c t a g a g

4440 A C T T T G T G C C T T C C T C T G T T TK-pA-R MLEFFAHPRGRLTETRKETI a t g c t g g a g t t c t t c g c c c a c c c t a g g g g g a g g c t a a c t g a a a c a c g g a a g g a g a c a a t a t a c g a c c t c a a g a a g c g g g t g g g a t c c c c c t c c g a t t g a c t t t g t g c c t t c c t c t g t t a t

4500 HSV TK poly(A) signal PEGTRAMTAIKRQNKTHGVG c c g g a a g g a a c c c g c g c t a t g a c g g c a a t a a a a a g a c a g a a t a a a a c g c a c g g t g t t g g g g g c c t t c c t t g g g c g c g a t a c t g c c g t t a t t t t t c t g t c t t a t t t t g c g t g c c a c a a c c c

4560 HSV TK poly(A) signal SFVHKRGVRSQGWHSVDTPP t c g t t t g t t c a t a a a c g c g g g g t t c g g t c c c a g g g c t g g c a c t c t g t c g a t a c c c c a c c g a g c a a a c a a g t a t t t g c g c c c c a a g c c a g g g t c c c g a c c g t g a g a c a g c t a t g g g g t g g c

4620 RPHWGQYARVSSFSPPHPPS a g a c c c c a t t g g g g c c a a t a c g c c c g c g t t t c t t c c t t t t c c c c a c c c c a c c c c c c a a g t t c t g g g g t a a c c c c g g t t a t g c g g g c g c a a a g a a g g a a a a g g g g t g g g g t g g g g g g t t c a

EcoO109I 4680 SGEGPGLAANVGAAGPAIAS t c g g g t g a a g g c c c a g g g c t c g c a g c c a a c g t c g g g g c g g c a g g c c c t g c c a t a g c c t c a a g c c c a c t t c c g g g t c c c g a g c g t c g g t t g c a g c c c c g c c g t c c g g g a c g g t a t c g g a g t

4740 GYSYIL* g g t t a c t c a t a t a t a c t t t a g a t t g a t t t a a a a c t t c a t t t t t a a t t t a a a a g g a t c t a g c c a a t g a g t a t a t a t g a a a t c t a a c t a a a t t t t g a a g t a a a a a t t a a a t t t t c c t a g a t c

4800 g t g a a g a t c c t t t t t g a t a a t c t c a t g a c c a a a a t c c c t t a a c g t g a g t t t t c g t t c c a c c a c t t c t a g g a a a a a c t a t t a g a g t a c t g g t t t t a g g g a a t t g c a c t c a a a a g c a a g g t g

4860 ori t g a g c g t c a g a c c c c g t a g a a a a g a t c a a a g g a t c t t c t t g a g a t c c t t t t t t t c t g c g c a c t c g c a g t c t g g g g c a t c t t t t c t a g t t t c c t a g a a g a a c t c t a g g a a a a a a a g a c g c g

4920 ori g t a a t c t g c t g c t t g c a a a c a a a a a a a c c a c c g c t a c c a g c g g t g g t t t g t t t g c c g g a t c a t t a g a c g a c g a a c g t t t g t t t t t t t g g t g g c g a t g g t c g c c a c c a a a c a a a c g g c c t a

4980 ori c a a g a g c t a c c a a c t c t t t t t c c g a a g g t a a c t g g c t t c a g c a g a g c g c a g a t a c c a a a t g t t c t c g a t g g t t g a g a a a a a g g c t t c c a t t g a c c g a a g t c g t c t c g c g t c t a t g g t t t a

5040 ori a c t g t t c t t c t a g t g t a g c c g t a g t t a g g c c a c c a c t t c a a g a a c t c t g t a g c a c c g c c t t g a c a a g a a g a t c a c a t c g g c a t c a a t c c g g t g g t g a a g t t c t t g a g a c a t c g t g g c g g a

5100 ori a c a t a c c t c g c t c t g c t a a t c c t g t t a c c a g t g g c t g c t g c c a g t g g c g a t a a g t c g t g t t g t a t g g a g c g a g a c g a t t a g g a c a a t g g t c a c c g a c g a c g g t c a c c g c t a t t c a g c a c a

5160 ori c t t a c c g g g t t g g a c t c a a g a c g a t a g t t a c c g g a t a a g g c g c a g c g g t c g g g c t g a a c g g a a t g g c c c a a c c t g a g t t c t g c t a t c a a t g g c c t a t t c c g c g t c g c c a g c c c g a c t t g c

ApaLI 5220 ori g g g g g t t c g t g c a c a c a g c c c a g c t t g g a g c g a a c g a c c t a c a c c g a a c t g a g a t a c c t a c c c c c a a g c a c g t g t g t c g g g t c g a a c c t c g c t t g c t g g a t g t g g c t t g a c t c t a t g g a t

5280 ori c a g c g t g a g c t a t g a g a a a g c g c c a c g c t t c c c g a a g g g a g a a a g g c g g a c a g g t a t c c g g t c g c a c t c g a t a c t c t t t c g c g g t g c g a a g g g c t t c c c t c t t t c c g c c t g t c c a t a g g c

5340 ori G G G A A A C G C C T G G pBR322ori-F g t a a g c g g c a g g g t c g g a a c a g g a g a g c g c a c g a g g g a g c t t c c a g g g g g a a a c g c c t g g c a t t c g c c g t c c c a g c c t t g t c c t c t c g c g t g c t c c c t c g a a g g t c c c c c t t t g c g g a c c

5400 ori T A T C T T T pBR322ori-F t a t c t t t a t a g t c c t g t c g g g t t t c g c c a c c t c t g a c t t g a g c g t c g a t t t t t g t g a t g c a t a g a a a t a t c a g g a c a g c c c a a a g c g g t g g a g a c t g a a c t c g c a g c t a a a a a c a c t a c g

5460 ori t c g t c a g g g g g g c g g a g c c t a t g g a a a a a c g c c a g c a a c g c g g c c t t t t t a c g g t t c c t g a g c a g t c c c c c c g c c t c g g a t a c c t t t t t g c g g t c g t t g c g c c g g a a a a a t g c c a a g g a c

PciI 5520 g c c t t t t g c t g g c c t t t t g c t c a c a t g t t c t t t c c t g c g t t a t c c c c t g a t t c t g t g g a t c g g a a a a c g a c c g g a a a a c g a g t g t a c a a g a a a g g a c g c a a t a g g g g a c t a a g a c a c c t a

3ʹ 5ʹ 5548 a a c c g t a t t a c c g c c a t g c a t t a g t t a t t t g g c a t a a t g g c g g t a c g t a a t c a a t a

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.