Addgene Flag-ECFP-betaPIXa Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: Flag-ECFP-betaPIXa Sequencing Result

Map View

800 600 400 200 End (823) EGFP-C (783 .. 804) BmrI (735) MflI * - BstYI (629) TatI (557) NmeAIII (485) EXFP-R (436 .. 455) PfoI * (410) BssSI - BssSαI (310) BsrFI (280) BtgZI (251) EaeI (201) EGFP-N (175 .. 196) BseRI (160) KpnI (131) Acc65I (127) HindIII (121) PpuMI - EcoO109I (115) BtgI - StyI - NcoI (86) BsaI (64) SfcI (63) T7 (51 .. 70) AseI (50) SacI - BanII (8) Eco53kI (6) Start (0) T7 promoter FLAG VN173 Kozak sequence Flag-ECFP-betaPIXa 823 bp

Sequence View

AseIStart (0) Eco53kIBanIISacI 5ʹ 3ʹ 60 T7 promoter T A A T A C G A C T T7 CLERAVLSGSSVPKDFNIRS g c a g a g c t c t c t g g c t a c t a g a g a a c c a c t g c t t a c t g g c t t a t c g a a a t t a a t a c g a c t c g t c t c g a g a g a c c g a t g a t c t c t t g g t g a c g a a t g a c c g a a t a g c t t t a a t t a t g c t g a

EcoO109IPpuMINcoIStyIBtgISfcIBsaI 120 1 5 DYKDDDDK GP T7 promoter FLAG Kozak sequence C A C T A T A G G G T7 MDYKDDDDKGP VIPLGLSGGHVVLVVIVLSR c a c t a t a g g g a g a c c c a a g c t g c c a c c a t g g a c t a c a a g g a c g a c g a t g a c a a g g g a c c t g t g a t a t c c c t c t g g g t t c g a c g g t g g t a c c t g a t g t t c c t g c t g c t a c t g t t c c c t g g a

BseRIHindIIIAcc65IKpnI 180 KLGT 1a V 5 10 15 SKGEELFTGVVPILV VN173 G A C C A G EGFP-N KLGTVSKGEELFTGVVPILV LKTGHALALLQEGPHHGDQD a a g c t t g g t a c c g t g a g c a a g g g c g a g g a g c t g t t c a c c g g g g t g g t g c c c a t c c t g g t c t t c g a a c c a t g g c a c t c g t t c c c g c t c c t c g a c a a g t g g c c c c a c c a c g g g t a g g a c c a g

EaeI 240 20 25 30 35 ELDGDVNGHKFSVSGEGEGD VN173 C T C G A C C T G C C G C T G C EGFP-N ELDGDVNGHKFSVSGEGEGD LQVAVYVAVLEAHGALALAI g a g c t g g a c g g c g a c g t a a a c g g c c a c a a g t t c a g c g t g t c c g g c g a g g g c g a g g g c g a t c t c g a c c t g c c g c t g c a t t t g c c g g t g t t c a a g t c g c a c a g g c c g c t c c c g c t c c c g c t a

BsrFIBtgZI 300 40 45 50 55 ATYGKLTLKFICTTGKLPVP VN173 ATYGKLTLKFICTTGKLPVP GGVALQGQLEDAGGALQGHG g c c a c c t a c g g c a a g c t g a c c c t g a a g t t c a t c t g c a c c a c c g g c a a g c t g c c c g t g c c c c g g t g g a t g c c g t t c g a c t g g g a c t t c a a g t a g a c g t g g t g g c c g t t c g a c g g g c a c g g g

BssSαIBssSI 360 60 65 70 75 WPTLVTTLTWGVQCFSRYPD VN173 WPTLVTTLTWGVQCFSRYPD PGGEHGGQGPAHLAEAAVGV t g g c c c a c c c t c g t g a c c a c c c t g a c c t g g g g c g t g c a g t g c t t c a g c c g c t a c c c c g a c a c c g g g t g g g a g c a c t g g t g g g a c t g g a c c c c g c a c g t c a c g a a g t c g g c g a t g g g g c t g

PfoI* 420 80 85 90 95 HMKQHDFFKSAMPEGYVQER VN173 HMKQHDFFKSAMPEGYVQER VHLLVVEELGGHGFAVDLLA c a c a t g a a g c a g c a c g a c t t c t t c a a g t c c g c c a t g c c c g a a g g c t a c g t c c a g g a g c g c g t g t a c t t c g t c g t g c t g a a g a a g t t c a g g c g g t a c g g g c t t c c g a t g c a g g t c c t c g c g

480 100 105 110 115 TIFFKDDGNYKTRAEVKFEG VN173 C T G C T G C C G T T G A T G T T C T G EXFP-R TIFFKDDGNYKTRAEVKFEG GDEELVVAVVLGAGLHLELA a c c a t c t t c t t c a a g g a c g a c g g c a a c t a c a a g a c c c g c g c c g a g g t g a a g t t c g a g g g c t g g t a g a a g a a g t t c c t g c t g c c g t t g a t g t t c t g g g c g c g g c t c c a c t t c a a g c t c c c g

NmeAIII 540 120 125 130 135 DTLVNRIELKGIDFKEDGNI VN173 DTLVNRIELKGIDFKEDGNI VGQHVADLQLADVELLVAVD g a c a c c c t g g t g a a c c g c a t c g a g c t g a a g g g c a t c g a c t t c a a g g a g g a c g g c a a c a t c c t g t g g g a c c a c t t g g c g t a g c t c g a c t t c c c g t a g c t g a a g t t c c t c c t g c c g t t g t a g

TatI 600 140 145 150 155 LGHKLEYNYISHNVYITADK VN173 LGHKLEYNYISHNVYITADK QPVLQLVVVDAVVDIDGGVL c t g g g g c a c a a g c t g g a g t a c a a c t a c a t c a g c c a c a a c g t c t a t a t c a c c g c c g a c a a g g a c c c c g t g t t c g a c c t c a t g t t g a t g t a g t c g g t g t t g c a g a t a t a g t g g c g g c t g t t c

BstYIMflI* 660 160 165 170 QKNGIKANFKIRHNIE DGSV VN173 QKNGIKANFKIRHNIEDGSV LLVADLGVELDAVVDLVAAH c a g a a g a a c g g c a t c a a g g c c a a c t t c a a g a t c c g c c a c a a c a t c g a g g a c g g c a g c g t g g t c t t c t t g c c g t a g t t c c g g t t g a a g t t c t a g g c g g t g t t g t a g c t c c t g c c g t c g c a c

720 (in frame with VN173) QLADHYQQNTPIGDGPVLLP QLADHYQQNTPIGDGPVLLP LEGVVVLLVGGDAVAGHQQG c a g c t c g c c g a c c a c t a c c a g c a g a a c a c c c c c a t c g g c g a c g g c c c c g t g c t g c t g c c c g t c g a g c g g c t g g t g a t g g t c g t c t t g t g g g g g t a g c c g c t g c c g g g g c a c g a c g a c g g g

BmrI 780 (in frame with VN173) DNHYLSTQSALSKDPNEKRD DNHYLSTQSALSKDPNEKRD VVVVQAGLGGQAFVGVLLAI g a c a a c c a c t a c c t g a g c a c c c a g t c c g c c c t g a g c a a a g a c c c c a a c g a g a a g c g c g a t c t g t t g g t g a t g g a c t c g t g g g t c a g g c g g g a c t c g t t t c t g g g g t t g c t c t t c g c g c t a

End (823) 3ʹ 5ʹ 823 (in frame with VN173) HMVLLEFVTAAGIT C A T G G T C C T G C T G G A G T T C G T G EGFP-C HMVLLEFVTAAGIT VHDQQLEHGGGPDS c a c a t g g t c c t g c t g g a g t t c g t g a c c g c c g c c g g g a t c a c t c g t g t a c c a g g a c g a c c t c a a g c a c t g g c g g c g g c c c t a g t g a g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.