Addgene 2xsspB-mSc-sspB-ITSN1 - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: 2xsspB-mSc-sspB-ITSN1


Map View

AflIII - PciI (6915) pBR322ori-F (6760 .. 6779) BsaI (5986) TK-pA-R (5851 .. 5870) Neo-F (5533 .. 5552) RsrII (5513) FspI (5099) Neo-R (4923 .. 4942) BsaBI * (4856) BspDI * - ClaI * (4837) SfiI (4772) CsiI - SexAI * (4586) pBABE 3' (4500 .. 4520) F1ori-R (4238 .. 4257) DraIII (4113) F1ori-F (4026 .. 4047) AflII (3879) EBV-rev (3852 .. 3871) BsmI (3841) SV40pA-R (3798 .. 3817) HpaI (3760) BsmBI - Esp3I (3604) AseI (7) CMV enhancer NdeI (234) SnaBI (340) CMV-F (519 .. 539) NheI (591) BmtI (595) PspOMI (653) TspMI - XmaI (656) ApaI (657) SmaI (658) AgeI (1496) BstEII (1652) mCherry-R (1642 .. 1660) AhdI (1691) mCherry-F (1910 .. 1929) NotI (2021) SgrAI (2178) XcmI (2183) BsrGI (2197) BspEI (2686) PmlI (3323) MfeI (3371) 2xsspB-mSc-sspB-ITSN1 6973 bp

Sequence View

AseI 5ʹ 3ʹ 60 t a g t t a t t a a t a g t a a t c a a t t a c g g g g t c a t t a g t t c a t a g c c c a t a t a t g g a g t t c c g a t c a a t a a t t a t c a t t a g t t a a t g c c c c a g t a a t c a a g t a t c g g g t a t a t a c c t c a a g g c

120 CMV enhancer c g t t a c a t a a c t t a c g g t a a a t g g c c c g c c t g g c t g a c c g c c c a a c g a c c c c c g c c c a t t g c a a t g t a t t g a a t g c c a t t t a c c g g g c g g a c c g a c t g g c g g g t t g c t g g g g g c g g g t a a

180 CMV enhancer g a c g t c a a t a a t g a c g t a t g t t c c c a t a g t a a c g c c a a t a g g g a c t t t c c a t t g a c g t c a c t g c a g t t a t t a c t g c a t a c a a g g g t a t c a t t g c g g t t a t c c c t g a a a g g t a a c t g c a g t

NdeI 240 CMV enhancer a t g g g t g g a g t a t t t a c g g t a a a c t g c c c a c t t g g c a g t a c a t c a a g t g t a t c a t a t g c c t a c c c a c c t c a t a a a t g c c a t t t g a c g g g t g a a c c g t c a t g t a g t t c a c a t a g t a t a c g g

300 CMV enhancer a a g t a c g c c c c c t a t t g a c g t c a a t g a c g g t a a a t g g c c c g c c t g g c a t t a t g c c c a g t a t t c a t g c g g g g g a t a a c t g c a g t t a c t g c c a t t t a c c g g g c g g a c c g t a a t a c g g g t c a t

SnaBI 360 CMV enhancer c a t g a c c t t a t g g g a c t t t c c t a c t t g g c a g t a c a t c t a c g t a t t a g t c a t c g c t a t t a c g t a c t g g a a t a c c c t g a a a g g a t g a a c c g t c a t g t a g a t g c a t a a t c a g t a g c g a t a a t g

420 CMV enhancer CMV promoter c a t g g t g a t g c g g t t t t g g c a g t a c a t c a a t g g g c g t g g a t a g c g g t t t g a c t c a c g g g g g t a c c a c t a c g c c a a a a c c g t c a t g t a g t t a c c c g c a c c t a t c g c c a a a c t g a g t g c c c c

480 CMV promoter a t t t c c a a g t c t c c a c c c c a t t g a c g t c a a t g g g a g t t t g t t t t g g c a c c a a a a t c a a c g t a a a g g t t c a g a g g t g g g g t a a c t g c a g t t a c c c t c a a a c a a a a c c g t g g t t t t a g t t g c

540 CMV promoter C G C A A A T G G G C G G T A G G C G T G CMV-F g g a c t t t c c a a a a t g t c g t a a c a a c t c c g c c c c a t t g a c g c a a a t g g g c g g t a g g c g t g t c c t g a a a g g t t t t a c a g c a t t g t t g a g g c g g g g t a a c t g c g t t t a c c c g c c a t c c g c a c a

BmtINheI 600 CMV promoter a c g g t g g g a g g t c t a t a t a a g c a g a g c t g g t t t a g t g a a c c g t c a g a t c c g c t a g c g c t a t g c c a c c c t c c a g a t a t a t t c g t c t c g a c c a a a t c a c t t g g c a g t c t a g g c g a t c g c g a t

PspOMITspMIXmaIApaISmaI 660 MCS c c g g a c t c a g a t c t c g a g c t c a a g c t t c g a a t t c t g c a g t c g a c g g t a c c g c g g g c c c g g g g c c t g a g t c t a g a g c t c g a g t t c g a a g c t t a a g a c g t c a g c t g c c a t g g c g c c c g g g c c

720 MCS MGLRSRAQASNEFGIDLS g a t c c a t g g g a c t c a g a t c t c g a g c t c a a g c t t c g a a c g a a t t c g g c a t t g a t c t g a g c g c t a g g t a c c c t g a g t c t a g a g c t c g a g t t c g a a g c t t g c t t a a g c c g t a a c t a g a c t c g c

780 5 10 15 SSPKRPKLLREYY SspB nano GLTLQEFSSPKRPKLLREYY g c c t g a c c c t g c a g g a a t t c a g c t c c c c g a a a c g c c c t a a g c t g c t g c g t g a a t a t t a c g c g g a c t g g g a c g t c c t t a a g t c g a g g g g c t t t g c g g g a t t c g a c g a c g c a c t t a t a a t g c

840 20 25 30 35 DWLVDNSFTPYLVVDATYLG SspB nano DWLVDNSFTPYLVVDATYLG a t t g g c t g g t t g a t a a c a g c t t t a c c c c a t a t c t g g t g g t g g a t g c c a c a t a c c t g g g c g t a a c c g a c c a a c t a t t g t c g a a a t g g g g t a t a g a c c a c c a c c t a c g g t g t a t g g a c c c g c

900 40 45 50 55 VNVPVEYVKDGQIVLNLSAS SspB nano VNVPVEYVKDGQIVLNLSAS t g a a c g t g c c c g t g g a g t a t g t g a a a g a c g g t c a g a t c g t g c t g a a t c t g t c t g c a a g t g a c t t g c a c g g g c a c c t c a t a c a c t t t c t g c c a g t c t a g c a c g a c t t a g a c a g a c g t t c a c

960 60 65 70 75 ATGNLQLTNDFIQFNARFKG SspB nano ATGNLQLTNDFIQFNARFKG c g a c c g g c a a c c t g c a a c t g a c a a a t g a t t t t a t c c a g t t c a a c g c c c g c t t t a a g g g c g g c t g g c c g t t g g a c g t t g a c t g t t t a c t a a a a t a g g t c a a g t t g c g g g c g a a a t t c c c g c

1020 80 85 90 95 VSRELYIPMGAALAIYAREN SspB nano VSRELYIPMGAALAIYAREN t g t c t c g t g a a c t g t a t a t c c c g a t g g g t g c c g c t c t g g c c a t t t a c g c t c g c g a g a a c g a c a g a g c a c t t g a c a t a t a g g g c t a c c c a c g g c g a g a c c g g t a a a t g c g a g c g c t c t t g c

1080 100 105 110 GDGVMFEPEEIYDELNI GPP SspB nano GDGVMFEPEEIYDELNIGPP g c g a t g g t g t g a t g t t c g a a c c a g a a g a a a t c t a t g a c g a g c t g a a t a t t g g t c c a c c g g c g c t a c c a c a c t a c a a g c t t g g t c t t c t t t a g a t a c t g c t c g a c t t a t a a c c a g g t g g c c

1140 (in frame with SspB nano) GSMGLRSRAQASNEFGIDLS GSMGLRSRAQASNEFGIDLS g a t c c a t g g g a c t c a g a t c t c g a g c t c a a g c t t c g a a c g a a t t c g g c a t t g a t c t g a g c g c t a g g t a c c c t g a g t c t a g a g c t c g a g t t c g a a g c t t g c t t a a g c c g t a a c t a g a c t c g c

1200 (in frame with SspB nano) GLTLQEF 5 10 15 SSPKRPKLLREYY SspB nano GLTLQEFSSPKRPKLLREYY g c c t g a c c c t g c a g g a a t t c a g c t c c c c g a a a c g c c c t a a g c t g c t g c g t g a a t a t t a c g c g g a c t g g g a c g t c c t t a a g t c g a g g g g c t t t g c g g g a t t c g a c g a c g c a c t t a t a a t g c

1260 20 25 30 35 DWLVDNSFTPYLVVDATYLG SspB nano DWLVDNSFTPYLVVDATYLG a t t g g c t g g t t g a t a a c a g c t t t a c c c c a t a t c t g g t g g t g g a t g c c a c a t a c c t g g g c g t a a c c g a c c a a c t a t t g t c g a a a t g g g g t a t a g a c c a c c a c c t a c g g t g t a t g g a c c c g c

1320 40 45 50 55 VNVPVEYVKDGQIVLNLSAS SspB nano VNVPVEYVKDGQIVLNLSAS t g a a c g t g c c c g t g g a g t a t g t g a a a g a c g g t c a g a t c g t g c t g a a t c t g t c t g c a a g t g a c t t g c a c g g g c a c c t c a t a c a c t t t c t g c c a g t c t a g c a c g a c t t a g a c a g a c g t t c a c

1380 60 65 70 75 ATGNLQLTNDFIQFNARFKG SspB nano ATGNLQLTNDFIQFNARFKG c g a c c g g c a a c c t g c a a c t g a c a a a t g a t t t t a t c c a g t t c a a c g c c c g c t t t a a g g g c g g c t g g c c g t t g g a c g t t g a c t g t t t a c t a a a a t a g g t c a a g t t g c g g g c g a a a t t c c c g c

1440 80 85 90 95 VSRELYIPMGAALAIYAREN SspB nano VSRELYIPMGAALAIYAREN t g t c t c g t g a a c t g t a t a t c c c g a t g g g t g c c g c t c t g g c c a t t t a c g c t c g c g a g a a c g a c a g a g c a c t t g a c a t a t a g g g c t a c c c a c g g c g a g a c c g g t a a a t g c g a g c g c t c t t g c

AgeI 1500 100 105 110 GDGVMFEPEEIYDELNI GPP SspB nano GDGVMFEPEEIYDELNIGPP g c g a t g g t g t g a t g t t c g a a c c a g a a g a a a t c t a t g a c g a g c t g a a t a t t g g t c c a c c g g c g c t a c c a c a c t a c a a g c t t g g t c t t c t t t a g a t a c t g c t c g a c t t a t a a c c a g g t g g c c

1560 VAT 1 5 10 15 MVSKGEAVIKEFMRFKV mScarlet Kozak sequence VATMVSKGEAVIKEFMRFKV t c g c c a c c a t g g t g a g c a a g g g c g a g g c a g t g a t c a a g g a g t t c a t g c g g t t c a a g g t g c a g c g g t g g t a c c a c t c g t t c c c g c t c c g t c a c t a g t t c c t c a a g t a c g c c a a g t t c c a c g

1620 20 25 30 35 HMEGSMNGHEFEIEGEGEGR mScarlet HMEGSMNGHEFEIEGEGEGR a c a t g g a g g g c t c c a t g a a c g g c c a c g a g t t c g a g a t c g a g g g c g a g g g c g a g g g c c g c c t g t a c c t c c c g a g g t a c t t g c c g g t g c t c a a g c t c t a g c t c c c g c t c c c g c t c c c g g c g g

BstEII 1680 40 45 50 55 PYEGTQTAKLKVTKGGPLPF mScarlet G G T T C G A C T T C C A C T G G T T mCherry-R PYEGTQTAKLKVTKGGPLPF c c t a c g a g g g c a c c c a g a c c g c c a a g c t g a a g g t g a c c a a g g g t g g c c c c c t g c c c t t c t g g a t g c t c c c g t g g g t c t g g c g g t t c g a c t t c c a c t g g t t c c c a c c g g g g g a c g g g a a g a

AhdI 1740 60 65 70 75 SWDILSPQFMYGSRAFTKHP mScarlet SWDILSPQFMYGSRAFTKHP c c t g g g a c a t c c t g t c c c c t c a g t t c a t g t a c g g c t c c a g g g c c t t c a c c a a g c a c c c c g g g a c c c t g t a g g a c a g g g g a g t c a a g t a c a t g c c g a g g t c c c g g a a g t g g t t c g t g g g g c

1800 80 85 90 95 ADIPDYYKQSFPEGFKWERV mScarlet ADIPDYYKQSFPEGFKWERV c c g a c a t c c c c g a c t a c t a t a a g c a g t c c t t c c c c g a g g g c t t c a a g t g g g a g c g c g t g a g g c t g t a g g g g c t g a t g a t a t t c g t c a g g a a g g g g c t c c c g a a g t t c a c c c t c g c g c a c t

1860 100 105 110 115 MNFEDGGAVTVTQDTSLEDG mScarlet MNFEDGGAVTVTQDTSLEDG t g a a c t t c g a g g a c g g c g g c g c c g t g a c c g t g a c c c a g g a c a c c t c c c t g g a g g a c g g c a a c t t g a a g c t c c t g c c g c c g c g g c a c t g g c a c t g g g t c c t g t g g a g g g a c c t c c t g c c g t

1920 120 125 130 135 TLIYKVKLRGTNFPPDGPVM mScarlet C C C C G T A A T G C mCherry-F TLIYKVKLRGTNFPPDGPVM c c c t g a t c t a c a a g g t g a a g c t c c g c g g c a c c a a c t t c c c t c c t g a c g g c c c c g t a a t g c g g g a c t a g a t g t t c c a c t t c g a g g c g c c g t g g t t g a a g g g a g g a c t g c c g g g g c a t t a c g

1980 140 145 150 155 QKKTMGWEASTERLYPEDGV mScarlet A G A A G A A G A mCherry-F QKKTMGWEASTERLYPEDGV a g a a g a a g a c a a t g g g c t g g g a a g c g t c c a c c g a g c g g t t g t a c c c c g a g g a c g g c g t g c t c t t c t t c t g t t a c c c g a c c c t t c g c a g g t g g c t c g c c a a c a t g g g g c t c c t g c c g c a c g

NotI 2040 160 165 170 175 LKGDIKMALRLKDGGRYLAD mScarlet LKGDIKMALRLKDGGRYLAD t g a a g g g c g a c a t t a a g a t g g c c c t g c g c c t g a a g g a c g g c g g c c g c t a c c t g g c g g a c t a c t t c c c g c t g t a a t t c t a c c g g g a c g c g g a c t t c c t g c c g c c g g c g a t g g a c c g c c t g a

2100 180 185 190 195 FKTTYKAKKPVQMPGAYNVD mScarlet FKTTYKAKKPVQMPGAYNVD t c a a g a c c a c c t a c a a g g c c a a g a a g c c c g t g c a g a t g c c c g g c g c c t a c a a c g t c g a c c a g t t c t g g t g g a t g t t c c g g t t c t t c g g g c a c g t c t a c g g g c c g c g g a t g t t g c a g c t g g

2160 200 205 210 215 RKLDITSHNEDYTVVEQYER mScarlet RKLDITSHNEDYTVVEQYER g c a a g t t g g a c a t c a c c t c c c a c a a c g a g g a c t a c a c c g t g g t g g a a c a g t a c g a a c g c t c g t t c a a c c t g t a g t g g a g g g t g t t g c t c c t g a t g t g g c a c c a c c t t g t c a t g c t t g c g a

BsrGIXcmISgrAI 2220 220 225 230 SEGRHSTGGMDELYK (in frame with mScarlet) GLRSR mScarlet SEGRHSTGGMDELYKGLRSR c c g a g g g c c g c c a c t c c a c c g g c g g c a t g g a c g a g c t g t a c a a g g g a c t c a g a t c t c g a g g g c t c c c g g c g g t g a g g t g g c c g c c g t a c c t g c t c g a c a t g t t c c c t g a g t c t a g a g c t c

2280 (in frame with mScarlet) AQASNEFGIDLSGLTLQEF 5 S SspB nano AQASNEFGIDLSGLTLQEFS c t c a a g c t t c g a a c g a a t t c g g c a t t g a t c t g a g c g g c c t g a c c c t g c a g g a a t t c a g c t g a g t t c g a a g c t t g c t t a a g c c g t a a c t a g a c t c g c c g g a c t g g g a c g t c c t t a a g t c g a

2340 10 15 20 25 SPKRPKLLREYYDWLVDNSF SspB nano SPKRPKLLREYYDWLVDNSF c c c c g a a a c g c c c t a a g c t g c t g c g t g a a t a t t a c g a t t g g c t g g t t g a t a a c a g c t t t a g g g g c t t t g c g g g a t t c g a c g a c g c a c t t a t a a t g c t a a c c g a c c a a c t a t t g t c g a a a t

2400 30 35 40 45 TPYLVVDATYLGVNVPVEYV SspB nano TPYLVVDATYLGVNVPVEYV c c c c a t a t c t g g t g g t g g a t g c c a c a t a c c t g g g c g t g a a c g t g c c c g t g g a g t a t g t g a g g g g t a t a g a c c a c c a c c t a c g g t g t a t g g a c c c g c a c t t g c a c g g g c a c c t c a t a c a c t

2460 50 55 60 65 KDGQIVLNLSASATGNLQLT SspB nano KDGQIVLNLSASATGNLQLT a a g a c g g t c a g a t c g t g c t g a a t c t g t c t g c a a g t g c g a c c g g c a a c c t g c a a c t g a c a a t t c t g c c a g t c t a g c a c g a c t t a g a c a g a c g t t c a c g c t g g c c g t t g g a c g t t g a c t g t t

2520 70 75 80 85 NDFIQFNARFKGVSRELYIP SspB nano NDFIQFNARFKGVSRELYIP a t g a t t t t a t c c a g t t c a a c g c c c g c t t t a a g g g c g t g t c t c g t g a a c t g t a t a t c c c g a t a c t a a a a t a g g t c a a g t t g c g g g c g a a a t t c c c g c a c a g a g c a c t t g a c a t a t a g g g c t

2580 90 95 100 105 MGAALAIYARENGDGVMFEP SspB nano MGAALAIYARENGDGVMFEP t g g g t g c c g c t c t g g c c a t t t a c g c t c g c g a g a a c g g c g a t g g t g t g a t g t t c g a a c c a g a c c c a c g g c g a g a c c g g t a a a t g c g a g c g c t c t t g c c g c t a c c a c a c t a c a a g c t t g g t c

2640 110 EEIYDELNI (in frame with SspB nano) GGTMGLRSRAQ SspB nano EEIYDELNIGGTMGLRSRAQ a a g a a a t c t a t g a c g a g c t g a a t a t t g g t g g t a c c a t g g g a c t c a g a t c t c g a g c t c a a g t t c t t t a g a t a c t g c t c g a c t t a t a a c c a c c a t g g t a c c c t g a g t c t a g a g c t c g a g t t c

BspEI 2700 (in frame with SspB nano) ASNEFGIDLSGLTLQVRNML ASNEFGIDLSGLTLQVRNML c t t c g a a c g a a t t c g g c a t t g a t c t g a g c g g c c t g a c c c t g c a g g t c c g g a a t a t g t t g a g a a g c t t g c t t a a g c c g t a a c t a g a c t c g c c g g a c t g g g a c g t c c a g g c c t t a t a c a a c t

2760 (in frame with SspB nano) TPTERKRQGYIHELIVTEEN TPTERKRQGYIHELIVTEEN c c c c a a c t g a a a g a a a g c g a c a a g g a t a c a t c c a c g a g c t c a t t g t c a c c g a g g a g a a c t g g g g t t g a c t t t c t t t c g c t g t t c c t a t g t a g g t g c t c g a g t a a c a g t g g c t c c t c t t g a

2820 (in frame with SspB nano) YVNDLQLVTEIFQKPLMESE YVNDLQLVTEIFQKPLMESE a t g t g a a t g a c c t g c a g c t g g t c a c a g a g a t t t t t c a a a a a c c c c t g a t g g a g t c t g a g c t a c a c t t a c t g g a c g t c g a c c a g t g t c t c t a a a a a g t t t t t g g g g a c t a c c t c a g a c t c g

2880 (in frame with SspB nano) LLTEKEVAMIFVNWKELIMC LLTEKEVAMIFVNWKELIMC t g c t g a c a g a a a a a g a g g t t g c t a t g a t t t t t g t g a a c t g g a a g g a g c t g a t t a t g t g t a a c g a c t g t c t t t t t c t c c a a c g a t a c t a a a a a c a c t t g a c c t t c c t c g a c t a a t a c a c a t

2940 (in frame with SspB nano) NIKLLKALRVRKKMSGEKMP NIKLLKALRVRKKMSGEKMP a t a t c a a a c t a c t a a a a g c g c t g a g a g t c c g c a a g a a g a t g t c c g g g g a g a a g a t g c c t g t a t a g t t t g a t g a t t t t c g c g a c t c t c a g g c g t t c t t c t a c a g g c c c c t c t t c t a c g g a c

3000 (in frame with SspB nano) VKMIGDILSAQLPHMQPYIR VKMIGDILSAQLPHMQPYIR t g a a g a t g a t t g g a g a c a t c c t g a g c g c a c a g c t g c c g c a c a t g c a g c c c t a c a t c c g c t a c t t c t a c t a a c c t c t g t a g g a c t c g c g t g t c g a c g g c g t g t a c g t c g g g a t g t a g g c g a

3060 (in frame with SspB nano) FCSRQLNGAALIQQKTDEAP FCSRQLNGAALIQQKTDEAP t c t g c a g c c g c c a g c t c a a c g g g g c t g c c c t g a t c c a g c a g a a g a c g g a c g a g g c c c c a g a g a c g t c g g c g g t c g a g t t g c c c c g a c g g g a c t a g g t c g t c t t c t g c c t g c t c c g g g g t c

3120 (in frame with SspB nano) DFKEFVKRLAMDPRCKGMPL DFKEFVKRLAMDPRCKGMPL a c t t c a a g g a g t t c g t c a a a a g a t t g g c a a t g g a t c c t c g g t g t a a a g g g a t g c c a c t c t t g a a g t t c c t c a a g c a g t t t t c t a a c c g t t a c c t a g g a g c c a c a t t t c c c t a c g g t g a g a

3180 (in frame with SspB nano) SSFILKPMQRVTRYPLIIKN SSFILKPMQRVTRYPLIIKN c t a g t t t t a t a c t g a a g c c t a t g c a a c g g g t a a c a a g a t a c c c a c t g a t c a t t a a a a a t a g a t c a a a a t a t g a c t t c g g a t a c g t t g c c c a t t g t t c t a t g g g t g a c t a g t a a t t t t t a t

3240 (in frame with SspB nano) ILENTPENHPDHSHLKHALE ILENTPENHPDHSHLKHALE t c c t g g a a a a c a c c c c t g a a a a c c a c c c g g a c c a c a g c c a c t t g a a g c a c g c c c t g g a g a a g g a c c t t t t g t g g g g a c t t t t g g t g g g c c t g g t g t c g g t g a a c t t c g t g c g g g a c c t c t

3300 (in frame with SspB nano) KAEELCSQVNEGVREKENSD KAEELCSQVNEGVREKENSD a g g c g g a a g a g c t c t g t t c c c a g g t g a a c g a a g g g g t g c g g g a g a a g g a g a a c t c t g a c c t c c g c c t t c t c g a g a c a a g g g t c c a c t t g c t t c c c c a c g c c c t c t t c c t c t t g a g a c t g g

PmlI 3360 (in frame with SspB nano) RLEWIQAHVQCEGLSEQLVF RLEWIQAHVQCEGLSEQLVF g g c t g g a g t g g a t c c a g g c c c a c g t g c a g t g t g a a g g c c t g t c t g a g c a a c t t g t g t t c a c c g a c c t c a c c t a g g t c c g g g t g c a c g t c a c a c t t c c g g a c a g a c t c g t t g a a c a c a a g t

MfeI 3420 (in frame with SspB nano) NSVTNCLGPRKFLHSGKLYK NSVTNCLGPRKFLHSGKLYK a t t c a g t g a c c a a t t g c t t g g g g c c g c g c a a a t t t c t g c a c a g t g g g a a g c t c t a c a a g g t a a g t c a c t g g t t a a c g a a c c c c g g c g c g t t t a a a g a c g t g t c a c c c t t c g a g a t g t t c c

3480 (in frame with SspB nano) AKSNKELYGFLFNDFLLLTQ AKSNKELYGFLFNDFLLLTQ c c a a g a g c a a c a a g g a g c t g t a t g g c t t c c t t t t c a a c g a c t t c c t c c t g c t g a c t c a g a g g t t c t c g t t g t t c c t c g a c a t a c c g a a g g a a a a g t t g c t g a a g g a g g a c g a c t g a g t c t

3540 (in frame with SspB nano) ITKPLGSSGTDKVFSPKSNL ITKPLGSSGTDKVFSPKSNL t c a c g a a g c c t t t g g g g t c t t c t g g c a c c g a c a a a g t c t t c a g c c c c a a a t c a a a c c t g c a g t g c t t c g g a a a c c c c a g a a g a c c g t g g c t g t t t c a g a a g t c g g g g t t t a g t t t g g a c g

3600 (in frame with SspB nano) QYKMYKTPIFLNEVLVKLPT QYKMYKTPIFLNEVLVKLPT a g t a t a a a a t g t a t a a a a c a c c t a t t t t c c t a a a t g a g g t t c t a g t a a a a t t a c c c a c c g t c a t a t t t t a c a t a t t t t g t g g a t a a a a g g a t t t a c t c c a a g a t c a t t t t a a t g g g t g g c

BsmBIEsp3I 3660 (in frame with SspB nano) DPSGDEPIFHISHIDRVYTL DPSGDEPIFHISHIDRVYTL a c c c t t c t g g a g a c g a g c c c a t c t t c c a c a t c t c c c a c a t t g a c c g c g t c t a t a c t c t c c t g g g a a g a c c t c t g c t c g g g t a g a a g g t g t a g a g g g t g t a a c t g g c g c a g a t a t g a g a g g

3720 (in frame with SspB nano) RAESINERTAWVQKIKAASE RAESINERTAWVQKIKAASE g a g c a g a a a g c a t a a a t g a a a g g a c t g c c t g g g t g c a g a a a a t c a a a g c t g c t t c t g a a c c t c g t c t t t c g t a t t t a c t t t c c t g a c g g a c c c a c g t c t t t t a g t t t c g a c g a a g a c t t g

HpaI 3780 (in frame with SspB nano) LYIETEKKKELLLLTCLLQL SV40 poly(A) signal LYIETEKKKELLLLTCLLQL t c t a c a t a g a g a c t g a g a a a a a g a a g g a a t t g t t g t t g t t a a c t t g t t t a t t g c a g c t t a a g a t g t a t c t c t g a c t c t t t t t c t t c c t t a a c a a c a a c a a t t g a a c a a a t a a c g t c g a a t

3840 (in frame with SspB nano) IMVTNKAIASQISQIKHFFH SV40 poly(A) signal C G T T A T C G T A G T G T T T A A A G SV40pA-R IMVTNKAIASQISQIKHFFH t a a t g g t t a c a a a t a a a g c a a t a g c a t c a c a a a t t t c a c a a a t a a a g c a t t t t t t t c a c t a t t a c c a a t g t t t a t t t c g t t a t c g t a g t g t t t a a a g t g t t t a t t t c g t a a a a a a a g t g a

AflIIBsmI 3900 (in frame with SspB nano) CILVVVCPNSSMYLKA* SV40 poly(A) signal f1 ori G T G G T T T G T C C A A A C T C A T C EBV-rev CILVVVCPNSSMYLKA* g c a t t c t a g t t g t g g t t t g t c c a a a c t c a t c a a t g t a t c t t a a g g c g t a a a t t g t a a g c g c g t a a g a t c a a c a c c a a a c a g g t t t g a g t a g t t a c a t a g a a t t c c g c a t t t a a c a t t c g c

3960 f1 ori t t a a t a t t t t g t t a a a a t t c g c g t t a a a t t t t t g t t a a a t c a g c t c a t t t t t t a a c c a a t a a t t a t a a a a c a a t t t t a a g c g c a a t t t a a a a a c a a t t t a g t c g a g t a a a a a a t t g g t t a

4020 f1 ori a g g c c g a a a t c g g c a a a a t c c c t t a t a a a t c a a a a g a a t a g a c c g a g a t a g g g t t g a g t g t c c g g c t t t a g c c g t t t t a g g g a a t a t t t a g t t t t c t t a t c t g g c t c t a t c c c a a c t c a c

4080 f1 ori G G T C A A A C C T T G T T C T C A G G T G F1ori-F t t g t t c c a g t t t g g a a c a a g a g t c c a c t a t t a a a g a a c g t g g a c t c c a a c g t c a a a g g g c a a c a a g g t c a a a c c t t g t t c t c a g g t g a t a a t t t c t t g c a c c t g a g g t t g c a g t t t c c c g

DraIII 4140 f1 ori g a a a a a c c g t c t a t c a g g g c g a t g g c c c a c t a c g t g a a c c a t c a c c c t a a t c a a g t t t t t c t t t t t g g c a g a t a g t c c c g c t a c c g g g t g a t g c a c t t g g t a g t g g g a t t a g t t c a a a a a

4200 f1 ori t g g g g t c g a g g t g c c g t a a a g c a c t a a a t c g g a a c c c t a a a g g g a g c c c c c g a t t t a g a g a c c c c a g c t c c a c g g c a t t t c g t g a t t t a g c c t t g g g a t t t c c c t c g g g g g c t a a a t c t c

4260 f1 ori A G G G A A G A A A G C G A A A G G A G F1ori-R c t t g a c g g g g a a a g c c g g c g a a c g t g g c g a g a a a g g a a g g g a a g a a a g c g a a a g g a g c g g g a a c t g c c c c t t t c g g c c g c t t g c a c c g c t c t t t c c t t c c c t t c t t t c g c t t t c c t c g c c

4320 f1 ori g c g c t a g g g c g c t g g c a a g t g t a g c g g t c a c g c t g c g c g t a a c c a c c a c a c c c g c c g c g c c g c g a t c c c g c g a c c g t t c a c a t c g c c a g t g c g a c g c g c a t t g g t g g t g t g g g c g g c g c g

4380 f1 ori AmpR promoter t t a a t g c g c c g c t a c a g g g c g c g t c a g g t g g c a c t t t t c g g g g a a a t g t g c g c g g a a c c c a a t t a c g c g g c g a t g t c c c g c g c a g t c c a c c g t g a a a a g c c c c t t t a c a c g c g c c t t g g g

4440 AmpR promoter c t a t t t g t t t a t t t t t c t a a a t a c a t t c a a a t a t g t a t c c g c t c a t g a g a c a a t a a c c c t g a t a a a c a a a t a a a a a g a t t t a t g t a a g t t t a t a c a t a g g c g a g t a c t c t g t t a t t g g g a

4500 AmpR promoter SV40 promoter SV40 enhancer C pBABE 3' g a t a a a t g c t t c a a t a a t a t t g a a a a a g g a a g a g t c c t g a g g c g g a a a g a a c c a g c t g t g c t a t t t a c g a a g t t a t t a t a a c t t t t t c c t t c t c a g g a c t c c g c c t t t c t t g g t c g a c a c

4560 SV40 promoter SV40 enhancer C T T A C A C A C A G T C A A T C C C A pBABE 3' g a a t g t g t g t c a g t t a g g g t g t g g a a a g t c c c c a g g c t c c c c a g c a g g c a g a a g t a t g c a c t t a c a c a c a g t c a a t c c c a c a c c t t t c a g g g g t c c g a g g g g t c g t c c g t c t t c a t a c g t

SexAI*CsiI 4620 SV40 promoter SV40 enhancer a a g c a t g c a t c t c a a t t a g t c a g c a a c c a g g t g t g g a a a g t c c c c a g g c t c c c c a g c a g g t t c g t a c g t a g a g t t a a t c a g t c g t t g g t c c a c a c c t t t c a g g g g t c c g a g g g g t c g t c c

4680 SV40 promoter SV40 enhancer c a g a a g t a t g c a a a g c a t g c a t c t c a a t t a g t c a g c a a c c a t a g t c c c g c c c c t a a c t c c g t c t t c a t a c g t t t c g t a c g t a g a g t t a a t c a g t c g t t g g t a t c a g g g c g g g g a t t g a g g

4740 SV40 promoter SV40 ori g c c c a t c c c g c c c c t a a c t c c g c c c a g t t c c g c c c a t t c t c c g c c c c a t g g c t g a c t a a t c g g g t a g g g c g g g g a t t g a g g c g g g t c a a g g c g g g t a a g a g g c g g g g t a c c g a c t g a t t a

SfiI 4800 SV40 promoter SV40 ori t t t t t t t a t t t a t g c a g a g g c c g a g g c c g c c t c g g c c t c t g a g c t a t t c c a g a a g t a g t g a a a a a a a t a a a t a c g t c t c c g g c t c c g g c g g a g c c g g a g a c t c g a t a a g g t c t t c a t c a c

BsaBI*BspDI*ClaI* 4860 SV40 promoter SV40 ori a g g a g g c t t t t t t g g a g g c c t a g g c t t t t g c a a a g a t c g a t c a a g a g a c a g g a t g a g g a t t c c t c c g a a a a a a c c t c c g g a t c c g a a a a c g t t t c t a g c t a g t t c t c t g t c c t a c t c c t a

4920 1 5 10 15 MIEQDGLHAGSPAAWVE NeoR/KanR 1 5 10 15 MIEQDGLHAGSPAAWVE NeoR/KanR MIEQDGLHAGSPAAWVE c g t t t c g c a t g a t t g a a c a a g a t g g a t t g c a c g c a g g t t c t c c g g c c g c t t g g g t g g a g a g c a a a g c g t a c t a a c t t g t t c t a c c t a a c g t g c g t c c a a g a g g c c g g c g a a c c c a c c t c t

4980 20 25 30 35 RLFGYDWAQQTIGCSDAAVF NeoR/KanR 20 25 30 35 RLFGYDWAQQTIGCSDAAVF NeoR/KanR G A T A A G C C G A T A C T G A C C C G Neo-R RLFGYDWAQQTIGCSDAAVF g g c t a t t c g g c t a t g a c t g g g c a c a a c a g a c a a t c g g c t g c t c t g a t g c c g c c g t g t t c c c c g a t a a g c c g a t a c t g a c c c g t g t t g t c t g t t a g c c g a c g a g a c t a c g g c g g c a c a a g g

5040 40 45 50 55 RLSAQGRPVLFVKTDLSGAL NeoR/KanR 40 45 50 55 RLSAQGRPVLFVKTDLSGAL NeoR/KanR RLSAQGRPVLFVKTDLSGAL g g c t g t c a g c g c a g g g g c g c c c g g t t c t t t t t g t c a a g a c c g a c c t g t c c g g t g c c c t g a c c g a c a g t c g c g t c c c c g c g g g c c a a g a a a a a c a g t t c t g g c t g g a c a g g c c a c g g g a c t

FspI 5100 60 65 70 75 NELQDEAARLSWLATTGVPC NeoR/KanR 60 65 70 75 NELQDEAARLSWLATTGVPC NeoR/KanR NELQDEAARLSWLATTGVPC a t g a a c t g c a a g a c g a g g c a g c g c g g c t a t c g t g g c t g g c c a c g a c g g g c g t t c c t t g c g t a c t t g a c g t t c t g c t c c g t c g c g c c g a t a g c a c c g a c c g g t g c t g c c c g c a a g g a a c g c

5160 80 85 90 95 AAVLDVVTEAGRDWLLLGEV NeoR/KanR 80 85 90 95 AAVLDVVTEAGRDWLLLGEV NeoR/KanR AAVLDVVTEAGRDWLLLGEV c a g c t g t g c t c g a c g t t g t c a c t g a a g c g g g a a g g g a c t g g c t g c t a t t g g g c g a a g t g c g t c g a c a c g a g c t g c a a c a g t g a c t t c g c c c t t c c c t g a c c g a c g a t a a c c c g c t t c a c g

5220 100 105 110 115 PGQDLLSSHLAPAEKVSIMA NeoR/KanR 100 105 110 115 PGQDLLSSHLAPAEKVSIMA NeoR/KanR PGQDLLSSHLAPAEKVSIMA *RVKSRGLFYGDHS c g g g g c a g g a t c t c c t g t c a t c t c a c c t t g c t c c t g c c g a g a a a g t a t c c a t c a t g g c t g g c c c c g t c c t a g a g g a c a g t a g a g t g g a a c g a g g a c g g c t c t t t c a t a g g t a g t a c c g a c

5280 120 125 130 135 DAMRRLHTLDPATCPFDHQA NeoR/KanR 120 125 130 135 DAMRRLHTLDPATCPFDHQA NeoR/KanR DAMRRLHTLDPATCPFDHQA ICHPPQMRKIRSGAWEVVLR a t g c a a t g c g g c g g c t g c a t a c g c t t g a t c c g g c t a c c t g c c c a t t c g a c c a c c a a g c g a t a c g t t a c g c c g c c g a c g t a t g c g a a c t a g g c c g a t g g a c g g g t a a g c t g g t g g t t c g c t

5340 140 145 150 155 KHRIERARTRMEAGLVDQDD NeoR/KanR 140 145 150 155 KHRIERARTRMEAGLVDQDD NeoR/KanR KHRIERARTRMEAGLVDQDD FMADLSCTSPHFGTKDILII a a c a t c g c a t c g a g c g a g c a c g t a c t c g g a t g g a a g c c g g t c t t g t c g a t c a g g a t g a t c t t g t a g c g t a g c t c g c t c g t g c a t g a g c c t a c c t t c g g c c a g a a c a g c t a g t c c t a c t a g

5400 160 165 170 175 LDEEHQGLAPAELFARLKAS NeoR/KanR 160 165 170 175 LDEEHQGLAPAELFARLKAS NeoR/KanR LDEEHQGLAPAELFARLKAS QVFLMLPERWGFQEGPELRA t g g a c g a a g a g c a t c a g g g g c t c g c g c c a g c c g a a c t g t t c g c c a g g c t c a a g g c g a g c a a c c t g c t t c t c g t a g t c c c c g a g c g c g g t c g g c t t g a c a a g c g g t c c g a g t t c c g c t c g t

5460 180 185 190 195 MPDGEDLVVTHGDACLPNIM NeoR/KanR 180 185 190 195 MPDGEDLVVTHGDACLPNIM NeoR/KanR MPDGEDLVVTHGDACLPNIM HGVALIEDHGMAIGAQRIDH t g c c c g a c g g c g a g g a t c t c g t c g t g a c c c a t g g c g a t g c c t g c t t g c c g a a t a t c a t g g a c g g g c t g c c g c t c c t a g a g c a g c a c t g g g t a c c g c t a c g g a c g a a c g g c t t a t a g t a c c

RsrII 5520 200 205 210 215 VENGRFSGFIDCGRLGVADR NeoR/KanR 200 205 210 215 VENGRFSGFIDCGRLGVADR NeoR/KanR VENGRFSGFIDCGRLGVADR HFIAAKRSEDVTAPQTHRVA t g g a a a a t g g c c g c t t t t c t g g a t t c a t c g a c t g t g g c c g g c t g g g t g t g g c g g a c c g c t a c c t t t t a c c g g c g a a a a g a c c t a a g t a g c t g a c a c c g g c c g a c c c a c a c c g c c t g g c g a

5580 220 225 230 235 YQDIALATRDIAEELGGEWA NeoR/KanR 220 225 230 235 YQDIALATRDIAEELGGEWA NeoR/KanR C G T T G G C T A C C C G T G A T A T T Neo-F YQDIALATRDIAEELGGEWA ILVYRQSGTINSFLKAAFPS a t c a g g a c a t a g c g t t g g c t a c c c g t g a t a t t g c t g a a g a g c t t g g c g g c g a a t g g g c t g t a g t c c t g t a t c g c a a c c g a t g g g c a c t a t a a c g a c t t c t c g a a c c g c c g c t t a c c c g a c

5640 240 245 250 255 DRFLVLYGIAAPDSQRIAFY NeoR/KanR 240 245 250 255 DRFLVLYGIAAPDSQRIAFY NeoR/KanR DRFLVLYGIAAPDSQRIAFY VAEEHKVTDGSGIRLADGEI a c c g c t t c c t c g t g c t t t a c g g t a t c g c c g c t c c c g a t t c g c a g c g c a t c g c c t t c t a t c t g g c g a a g g a g c a c g a a a t g c c a t a g c g g c g a g g g c t a a g c g t c g c g t a g c g g a a g a t a g

5700 260 265 RLLDEFF* NeoR/KanR 260 265 RLLDEFF* NeoR/KanR MTDQAT RLLDEFF* AKKVLEESRSEPTRFSRGLS g c c t t c t t g a c g a g t t c t t c t g a g c g g g a c t c t g g g g t t c g a a a t g a c c g a c c a a g c g a c c g g a a g a a c t g c t c a a g a a g a c t c g c c c t g a g a c c c c a a g c t t t a c t g g c t g g t t c g c t g

5760 PNLPSRDFDSTAAFYERLGF AWGAM g c c c a a c c t g c c a t c a c g a g a t t t c g a t t c c a c c g c c g c c t t c t a t g a a a g g t t g g g c t t c g g g t t g g a c g g t a g t g c t c t a a a g c t a a g g t g g c g g c g g a a g a t a c t t t c c a a c c c g a a

5820 GIVFRDAGWMILQRGDLMLE c g g a a t c g t t t t c c g g g a c g c c g g c t g g a t g a t c c t c c a g c g c g g g g a t c t c a t g c t g g a g c c t t a g c a a a a g g c c c t g c g g c c g a c c t a c t a g g a g g t c g c g c c c c t a g a g t a c g a c c t

5880 A C T T T G T G C C T T C C T C T G T T TK-pA-R FFAHPRGRLTETRKETIPEG g t t c t t c g c c c a c c c t a g g g g g a g g c t a a c t g a a a c a c g g a a g g a g a c a a t a c c g g a a g g c a a g a a g c g g g t g g g a t c c c c c t c c g a t t g a c t t t g t g c c t t c c t c t g t t a t g g c c t t c c

5940 HSV TK poly(A) signal TRAMTAIKRQNKTHGVGSFV a a c c c g c g c t a t g a c g g c a a t a a a a a g a c a g a a t a a a a c g c a c g g t g t t g g g t c g t t t g t t t g g g c g c g a t a c t g c c g t t a t t t t t c t g t c t t a t t t t g c g t g c c a c a a c c c a g c a a a c a

BsaI 6000 HSV TK poly(A) signal HKRGVRSQGWHSVDTPPRPH t c a t a a a c g c g g g g t t c g g t c c c a g g g c t g g c a c t c t g t c g a t a c c c c a c c g a g a c c c c a a g t a t t t g c g c c c c a a g c c a g g g t c c c g a c c g t g a g a c a g c t a t g g g g t g g c t c t g g g g t

6060 WGQYARVSSFSPPHPPSSGE t t g g g g c c a a t a c g c c c g c g t t t c t t c c t t t t c c c c a c c c c a c c c c c c a a g t t c g g g t g a a a c c c c g g t t a t g c g g g c g c a a a g a a g g a a a a g g g g t g g g g t g g g g g g t t c a a g c c c a c t

6120 GPGLAANVGAAGPAIASGYS a g g c c c a g g g c t c g c a g c c a a c g t c g g g g c g g c a g g c c c t g c c a t a g c c t c a g g t t a c t c t c c g g g t c c c g a g c g t c g g t t g c a g c c c c g c c g t c c g g g a c g g t a t c g g a g t c c a a t g a g

6180 YIL* a t a t a t a c t t t a g a t t g a t t t a a a a c t t c a t t t t t a a t t t a a a a g g a t c t a g g t g a a g a t t a t a t a t g a a a t c t a a c t a a a t t t t g a a g t a a a a a t t a a a t t t t c c t a g a t c c a c t t c t a

6240 c c t t t t t g a t a a t c t c a t g a c c a a a a t c c c t t a a c g t g a g t t t t c g t t c c a c t g a g c g t c g g a a a a a c t a t t a g a g t a c t g g t t t t a g g g a a t t g c a c t c a a a a g c a a g g t g a c t c g c a g

6300 ori a g a c c c c g t a g a a a a g a t c a a a g g a t c t t c t t g a g a t c c t t t t t t t c t g c g c g t a a t c t g t c t g g g g c a t c t t t t c t a g t t t c c t a g a a g a a c t c t a g g a a a a a a a g a c g c g c a t t a g a c

6360 ori c t g c t t g c a a a c a a a a a a a c c a c c g c t a c c a g c g g t g g t t t g t t t g c c g g a t c a a g a g c t g a c g a a c g t t t g t t t t t t t g g t g g c g a t g g t c g c c a c c a a a c a a a c g g c c t a g t t c t c g a

6420 ori a c c a a c t c t t t t t c c g a a g g t a a c t g g c t t c a g c a g a g c g c a g a t a c c a a a t a c t g t c c t t g g t t g a g a a a a a g g c t t c c a t t g a c c g a a g t c g t c t c g c g t c t a t g g t t t a t g a c a g g a

6480 ori t c t a g t g t a g c c g t a g t t a g g c c a c c a c t t c a a g a a c t c t g t a g c a c c g c c t a c a t a c c t a g a t c a c a t c g g c a t c a a t c c g g t g g t g a a g t t c t t g a g a c a t c g t g g c g g a t g t a t g g a

6540 ori c g c t c t g c t a a t c c t g t t a c c a g t g g c t g c t g c c a g t g g c g a t a a g t c g t g t c t t a c c g g g c g a g a c g a t t a g g a c a a t g g t c a c c g a c g a c g g t c a c c g c t a t t c a g c a c a g a a t g g c c

6600 ori g t t g g a c t c a a g a c g a t a g t t a c c g g a t a a g g c g c a g c g g t c g g g c t g a a c g g g g g g t t c c a a c c t g a g t t c t g c t a t c a a t g g c c t a t t c c g c g t c g c c a g c c c g a c t t g c c c c c c a a g

6660 ori g t g c a c a c a g c c c a g c t t g g a g c g a a c g a c c t a c a c c g a a c t g a g a t a c c t a c a g c g t g a c a c g t g t g t c g g g t c g a a c c t c g c t t g c t g g a t g t g g c t t g a c t c t a t g g a t g t c g c a c t

6720 ori g c t a t g a g a a a g c g c c a c g c t t c c c g a a g g g a g a a a g g c g g a c a g g t a t c c g g t a a g c g g c g a t a c t c t t t c g c g g t g c g a a g g g c t t c c c t c t t t c c g c c t g t c c a t a g g c c a t t c g c c

6780 ori G G G A A A C G C C T G G T A T C T T T pBR322ori-F c a g g g t c g g a a c a g g a g a g c g c a c g a g g g a g c t t c c a g g g g g a a a c g c c t g g t a t c t t t a g t c c c a g c c t t g t c c t c t c g c g t g c t c c c t c g a a g g t c c c c c t t t g c g g a c c a t a g a a a t

6840 ori t a g t c c t g t c g g g t t t c g c c a c c t c t g a c t t g a g c g t c g a t t t t t g t g a t g c t c g t c a g g a t c a g g a c a g c c c a a a g c g g t g g a g a c t g a a c t c g c a g c t a a a a a c a c t a c g a g c a g t c c

6900 ori g g g g c g g a g c c t a t g g a a a a a c g c c a g c a a c g c g g c c t t t t t a c g g t t c c t g g c c t t t t g c c c c g c c t c g g a t a c c t t t t t g c g g t c g t t g c g c c g g a a a a a t g c c a a g g a c c g g a a a a c

AflIIIPciI 6960 c t g g c c t t t t g c t c a c a t g t t c t t t c c t g c g t t a t c c c c t g a t t c t g t g g a t a a c c g t a t g a c c g g a a a a c g a g t g t a c a a g a a a g g a c g c a a t a g g g g a c t a a g a c a c c t a t t g g c a t a

3ʹ 5ʹ 6973 t a c c g c c a t g c a t a t g g c g g t a c g t a

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.