Addgene pBABE.puro/TO/Flag/HuR.S221D Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pBABE.puro/TO/Flag/HuR.S221D Sequencing Result


Map View

XmnI (6662) Amp-R (6613 .. 6632) ScaI (6543) PvuI (6433) FspI (6285) NotI (5942) pBR322ori-F (5322 .. 5341) L4440 (5071 .. 5088) BspQI - SapI (5065) BmtI (4307) NheI (4303) BspDI - ClaI (4229) DraIII (4146) Puro-F (4071 .. 4091) RsrII (3691) BsiWI (3631) Puro-R (3575 .. 3594) AvrII (3550) SspI (6867) SpeI (616) AfeI (879) gag (truncated) pLXSN 5' (1288 .. 1310) pBABE 5' (1326 .. 1342) NgoMIV (1356) NaeI (1358) BsaBI * (1383) SalI - SgrDI (1389) AccI (1390) LNCX (1396 .. 1420) PmeI (1498) PmlI (1734) SgrAI (1918) BmgBI (2025) XcmI (2103) BstXI (2203) BamHI (3011) BtgZI (3148) pBABE 3' (3231 .. 3251) SfiI (3503) pBABE.puro/TO/Flag/HuR.S221D 6978 bp

Sequence View

60 LTR c t g c a g c c t g a a t a t g g g c c a a a c a g g a t a t c t g t g g t a a g c a g t t c c t g c c c c g g c t c a g a c g t c g g a c t t a t a c c c g g t t t g t c c t a t a g a c a c c a t t c g t c a a g g a c g g g g c c g a g t

120 LTR g g g c c a a g a a c a g a t g g a a c a g c t g a a t a t g g g c c a a a c a g g a t a t c t g t g g t a a g c a g t c c c g g t t c t t g t c t a c c t t g t c g a c t t a t a c c c g g t t t g t c c t a t a g a c a c c a t t c g t c a

180 LTR t c c t g c c c c g g c t c a g g g c c a a g a a c a g a t g g t c c c c a g a t g c g g t c c a g c c c t c a g c a g a g g a c g g g g c c g a g t c c c g g t t c t t g t c t a c c a g g g g t c t a c g c c a g g t c g g g a g t c g t c

240 LTR t t t c t a g a g a a c c a t c a g a t g t t t c c a g g g t g c c c c a a g g a c c t g a a a t g a c c c t g t g c c a a a g a t c t c t t g g t a g t c t a c a a a g g t c c c a c g g g g t t c c t g g a c t t t a c t g g g a c a c g g

300 LTR t t a t t t g a a c t a a c c a a t c a g t t c g c t t c t c g c t t c t g t t c g c g c g c t t c t g c t c c c c g a a a t a a a c t t g a t t g g t t a g t c a a g c g a a g a g c g a a g a c a a g c g c g c g a a g a c g a g g g g c t

360 LTR g c t c a a t a a a a g a g c c c a c a a c c c c t c a c t c g g g g c g c c a g t c c t c c g a t t g a c t g a g t c c g a g t t a t t t t c t c g g g t g t t g g g g a g t g a g c c c c g c g g t c a g g a g g c t a a c t g a c t c a g

420 LTR g c c c g g g t a c c c g t g t a t c c a a t a a a c c c t c t t g c a g t t g c a t c c g a c t t g t g g t c t c g c c g g g c c c a t g g g c a c a t a g g t t a t t t g g g a g a a c g t c a a c g t a g g c t g a a c a c c a g a g c g

480 LTR t g t t c c t t g g g a g g g t c t c c t c t g a g t g a t t g a c t a c c c g t c a g c g g g g g t c t t t c a t t t a c a a g g a a c c c t c c c a g a g g a g a c t c a c t a a c t g a t g g g c a g t c g c c c c c a g a a a g t a a a

540 g g g g g c t c g t c c g g g a t c g g g a g a c c c c t g c c c a g g g a c c a c c g a c c c a c c a c c g g g a g g c c c c c g a g c a g g c c c t a g c c c t c t g g g g a c g g g t c c c t g g t g g c t g g g t g g t g g c c c t c c

600 MMLV Ψ c a a g c t g g c c a g c a a c t t a t c t g t g t c t g t c c g a t t g t c t a g t g t c t a t g a c t g a t t t t a g t t c g a c c g g t c g t t g a a t a g a c a c a g a c a g g c t a a c a g a t c a c a g a t a c t g a c t a a a a t

SpeI 660 MMLV Ψ t g c g c c t g c g t c g g t a c t a g t t a g c t a a c t a g c t c t g t a t c t g g c g g a c c c g t g g t g g a a a c g c g g a c g c a g c c a t g a t c a a t c g a t t g a t c g a g a c a t a g a c c g c c t g g g c a c c a c c t t

720 MMLV Ψ c t g a c g a g t t c t g a a c a c c c g g c c g c a a c c c t g g g a g a c g t c c c a g g g a c t t t g g g g g c c g a c t g c t c a a g a c t t g t g g g c c g g c g t t g g g a c c c t c t g c a g g g t c c c t g a a a c c c c c g g

780 MMLV Ψ g t t t t t g t g g c c c g a c c t g a g g a a g g g a g t c g a t g t g g a a t c c g a c c c c g t c a g g a t a t g c a a a a a c a c c g g g c t g g a c t c c t t c c c t c a g c t a c a c c t t a g g c t g g g g c a g t c c t a t a c

840 t g g t t c t g g t a g g a g a c g a g a a c c t a a a a c a g t t c c c g c c t c c g t c t g a a t t t t t g c t t t a c c a a g a c c a t c c t c t g c t c t t g g a t t t t g t c a a g g g c g g a g g c a g a c t t a a a a a c g a a a

AfeI 900 c g g t t t g g a a c c g a a g c c g c g c g t c t t g t c t g c t g c a g c g c t g c a g c a t c g t t c t g t g t t g c c a a a c c t t g g c t t c g g c g c g c a g a a c a g a c g a c g t c g c g a c g t c g t a g c a a g a c a c a a

960 gag (truncated) g t c t c t g t c t g a c t g t g t t t c t g t a t t t g t c t g a a a a t t a g g g c c a g a c t g t t a c c a c t c c a g a g a c a g a c t g a c a c a a a g a c a t a a a c a g a c t t t t a a t c c c g g t c t g a c a a t g g t g a g

1020 gag (truncated) c c t t a a g t t t g a c c t t a g g t c a c t g g a a a g a t g t c g a g c g g a t c g c t c a c a a c c a g t c g g g g a a t t c a a a c t g g a a t c c a g t g a c c t t t c t a c a g c t c g c c t a g c g a g t g t t g g t c a g c c

1080 gag (truncated) t a g a t g t c a a g a a g a g a c g t t g g g t t a c c t t c t g c t c t g c a g a a t g g c c a a c c t t t a a c g a t c t a c a g t t c t t c t c t g c a a c c c a a t g g a a g a c g a g a c g t c t t a c c g g t t g g a a a t t g c

1140 gag (truncated) t c g g a t g g c c g c g a g a c g g c a c c t t t a a c c g a g a c c t c a t c a c c c a g g t t a a g a t c a a g g a g c c t a c c g g c g c t c t g c c g t g g a a a t t g g c t c t g g a g t a g t g g g t c c a a t t c t a g t t c c

1200 gag (truncated) t c t t t t c a c c t g g c c c g c a t g g a c a c c c a g a c c a g g t c c c c t a c a t c g t g a c c t g g g a a g a g a a a a g t g g a c c g g g c g t a c c t g t g g g t c t g g t c c a g g g g a t g t a g c a c t g g a c c c t t c

1260 gag (truncated) c c t t g g c t t t t g a c c c c c c t c c c t g g g t c a a g c c c t t t g t a c a c c c t a a g c c t c c g c c t c g g a a c c g a a a a c t g g g g g g a g g g a c c c a g t t c g g g a a a c a t g t g g g a t t c g g a g g c g g a g

1320 gag (truncated) C C C T T G A A C C T C C T C G T T C G A C C pLXSN 5' c t c t t c c t c c a t c c g c c c c g t c t c t c c c c c t t g a a c c t c c t c g t t c g a c c c c g c c t c g a t g a g a a g g a g g t a g g c g g g g c a g a g a g g g g g a a c t t g g a g g a g c a a g c t g g g g c g g a g c t a

NgoMIVNaeI 1380 gag (truncated) tet operator C T T T A T C C A G C C C T C A C pBABE 5' c c t c c c t t t a t c c a g c c c t c a c t c c t t c t c t a g g c g c c g g c c g g a t c t c c c t a t c a g t g a g g a g g g a a a t a g g t c g g g a g t g a g g a a g a g a t c c g c g g c c g g c c t a g a g g g a t a g t c a c t

BsaBI*SgrDISalIAccI 1440 tet operator A G C T C G T T T A G T G A A C C G T C A G A T C LNCX t a g a g a t c g t c g a c g a g c t c g t t t a g t g a a c c g t c a g a t c g c c t g g a g a c g c c a t c c a c g a t c t c t a g c a g c t g c t c g a g c a a a t c a c t t g g c a g t c t a g c g g a c c t c t g c g g t a g g t g c

PmeI 1500 c t g t t t t g a c c t c c a t a g a a g a c a c c g g g a c c g a t c c a g c c t c c g g a c t c t a g c g t t t a a g a c a a a a c t g g a g g t a t c t t c t g t g g c c c t g g c t a g g t c g g a g g c c t g a g a t c g c a a a t t

1560 1 5 DYKDDDDK KL 1 MVSV FLAG mKO2 1 MVSV Kozak sequence hmKO MDYKDDDDKKLMVSV a c t t a a g c t g c c a c c a t g g a c t a c a a g g a c g a c g a t g a c a a g a a g c t t a t g g t g a g c g t g t g a a t t c g a c g g t g g t a c c t g a t g t t c c t g c t g c t a c t g t t c t t c g a a t a c c a c t c g c a c

1620 5 10 15 20 IKPEMKMRYYMDGSVNGHEF hmKO 5 10 15 20 IKPEMKMRYYMDGSVNGHEF mKO2 IKPEMKMRYYMDGSVNGHEF a t c a a g c c c g a g a t g a a g a t g a g g t a c t a c a t g g a c g g c t c c g t c a a t g g g c a t g a g t t c t a g t t c g g g c t c t a c t t c t a c t c c a t g a t g t a c c t g c c g a g g c a g t t a c c c g t a c t c a a g

1680 25 30 35 40 TIEGEGTGRPYEGHQEMTLR hmKO 25 30 35 40 TIEGEGTGRPYEGHQEMTLR mKO2 TIEGEGTGRPYEGHQEMTLR a c a a t c g a g g g t g a g g g c a c a g g c a g a c c t t a c g a g g g a c a t c a g g a g a t g a c a c t g c g c t g t t a g c t c c c a c t c c c g t g t c c g t c t g g a a t g c t c c c t g t a g t c c t c t a c t g t g a c g c g

PmlI 1740 45 50 55 60 VTMAEGGPMPFAFDLVSHVF hmKO 45 50 55 60 VTMAEGGPMPFAFDLVSHVF mKO2 VTMAEGGPMPFAFDLVSHVF g t c a c a a t g g c c g a g g g c g g g c c a a t g c c t t t c g c c t t c g a c c t g g t g t c c c a c g t g t t c c a g t g t t a c c g g c t c c c g c c c g g t t a c g g a a a g c g g a a g c t g g a c c a c a g g g t g c a c a a g

1800 65 70 75 80 CYGHRVFTKYPEEIPDYFKQ hmKO 65 70 75 80 CYGHRVFTKYPEEIPDYFKQ mKO2 CYGHRVFTKYPEEIPDYFKQ t g t t a c g g c c a c a g a g t t t t t a c c a a g t a c c c a g a a g a g a t c c c a g a c t a t t t c a a g c a g a c a a t g c c g g t g t c t c a a a a a t g g t t c a t g g g t c t t c t c t a g g g t c t g a t a a a g t t c g t c

1860 85 90 95 100 AFPEGLSWERSLEFEDGGSA hmKO 85 90 95 100 AFPEGLSWERSLEFEDGGSA mKO2 AFPEGLSWERSLEFEDGGSA g c c t t t c c t g a g g g c c t g t c c t g g g a g a g g t c c c t g g a g t t c g a g g a c g g c g g c t c c g c c c g g a a a g g a c t c c c g g a c a g g a c c c t c t c c a g g g a c c t c a a g c t c c t g c c g c c g a g g c g g

SgrAI 1920 105 110 115 120 SVSAHISLRGNTFYHKSKFT hmKO 105 110 115 120 SVSAHISLRGNTFYHKSKFT mKO2 SVSAHISLRGNTFYHKSKFT t c c g t g a g c g c c c a c a t c a g c c t g a g g g g c a a c a c c t t c t a c c a c a a g t c c a a g t t c a c c a g g c a c t c g c g g g t g t a g t c g g a c t c c c c g t t g t g g a a g a t g g t g t t c a g g t t c a a g t g g

1980 125 130 135 140 GVNFPADGPIMQNQSVDWEP hmKO 125 130 135 140 GVNFPADGPIMQNQSVDWEP mKO2 GVNFPADGPIMQNQSVDWEP g g c g t g a a c t t c c c c g c c g a c g g c c c c a t c a t g c a g a a c c a g a g c g t g g a c t g g g a g c c c c c g c a c t t g a a g g g g c g g c t g c c g g g g t a g t a c g t c t t g g t c t c g c a c c t g a c c c t c g g g

BmgBI 2040 145 150 155 160 STEKITASDGVLKGDVTMYL hmKO 145 150 155 160 STEKITASDGVLKGDVTMYL mKO2 STEKITASDGVLKGDVTMYL t c c a c c g a g a a g a t c a c c g c c a g c g a c g g c g t g c t g a a g g g c g a c g t g a c c a t g t a c c t g a g g t g g c t c t t c t a g t g g c g g t c g c t g c c g c a c g a c t t c c c g c t g c a c t g g t a c a t g g a c

2100 165 170 175 180 KLEGGGNHKCQMKTTYKAAK hmKO 165 170 175 180 KLEGGGNHKCQMKTTYKAAK mKO2 KLEGGGNHKCQMKTTYKAAK a a g c t g g a g g g c g g c g g c a a c c a c a a g t g c c a g a t g a a g a c c a c c t a c a a g g c c g c c a a g t t c g a c c t c c c g c c g c c g t t g g t g t t c a c g g t c t a c t t c t g g t g g a t g t t c c g g c g g t t c

XcmI 2160 185 190 195 200 EILEMPGDHYIGHRLVRKTE hmKO 185 190 195 200 EILEMPGDHYIGHRLVRKTE mKO2 EILEMPGDHYIGHRLVRKTE g a g a t c c t g g a g a t g c c c g g c g a c c a c t a c a t c g g c c a c a g g c t g g t g a g g a a g a c c g a g c t c t a g g a c c t c t a c g g g c c g c t g g t g a t g t a g c c g g t g t c c g a c c a c t c c t t c t g g c t c

BstXI 2220 205 210 215 GNITEQVEDAVAHS (in frame with hmKO) GGGGSG hmKO 205 210 215 GNITEQVEDAVAHS (in frame with mKO2) GGGGSG mKO2 GNITEQVEDAVAHSGGGGSG g g c a a c a t c a c c g a g c a g g t g g a g g a c g c c g t g g c c c a c t c c g g t g g c g g a g g c t c g g g c c c g t t g t a g t g g c t c g t c c a c c t c c t g c g g c a c c g g g t g a g g c c a c c g c c t c c g a g c c c g

2280 (in frame with hmKO) GGGSGTMSNGYEDHMAEDCR (in frame with mKO2) GGGSGTMSNGYEDHMAEDCR GGGSGTMSNGYEDHMAEDCR g g a g g t g g g t c g g g t a c c a t g t c t a a t g g t t a t g a a g a c c a c a t g g c c g a a g a c t g c a g g c c t c c a c c c a g c c c a t g g t a c a g a t t a c c a a t a c t t c t g g t g t a c c g g c t t c t g a c g t c c

2340 (in frame with hmKO) GDIGRTNLIVNYLPQNMTQD (in frame with mKO2) GDIGRTNLIVNYLPQNMTQD GDIGRTNLIVNYLPQNMTQD g g t g a c a t c g g g a g a a c g a a t t t g a t c g t c a a c t a c c t c c c t c a g a a c a t g a c c c a g g a t c c a c t g t a g c c c t c t t g c t t a a a c t a g c a g t t g a t g g a g g g a g t c t t g t a c t g g g t c c t a

2400 (in frame with hmKO) ELRSLFSSIGEVESAKLIRD (in frame with mKO2) ELRSLFSSIGEVESAKLIRD ELRSLFSSIGEVESAKLIRD g a g t t a c g a a g c c t g t t c a g c a g c a t t g g t g a a g t t g a a t c t g c a a a a c t t a t t c g g g a t c t c a a t g c t t c g g a c a a g t c g t c g t a a c c a c t t c a a c t t a g a c g t t t t g a a t a a g c c c t a

2460 (in frame with hmKO) KVAGHSLGYGFVNYVTAKDA (in frame with mKO2) KVAGHSLGYGFVNYVTAKDA KVAGHSLGYGFVNYVTAKDA a a a g t a g c a g g a c a c a g c t t g g g c t a t g g c t t t g t g a a c t a c g t g a c c g c g a a g g a t g c a t t t c a t c g t c c t g t g t c g a a c c c g a t a c c g a a a c a c t t g a t g c a c t g g c g c t t c c t a c g t

2520 (in frame with hmKO) ERAINTLNGLRLQSKTIKVS (in frame with mKO2) ERAINTLNGLRLQSKTIKVS ERAINTLNGLRLQSKTIKVS g a g a g a g c g a t c a a c a c g c t g a a c g g c t t g a g g c t c c a g t c a a a a a c c a t t a a g g t g t c g c t c t c t c g c t a g t t g t g c g a c t t g c c g a a c t c c g a g g t c a g t t t t t g g t a a t t c c a c a g c

2580 (in frame with hmKO) YARPSSEVIKDANLYISGLP (in frame with mKO2) YARPSSEVIKDANLYISGLP YARPSSEVIKDANLYISGLP t a t g c t c g c c c g a g c t c a g a g g t g a t c a a a g a c g c c a a c t t g t a c a t c a g c g g g c t c c c g a t a c g a g c g g g c t c g a g t c t c c a c t a g t t t c t g c g g t t g a a c a t g t a g t c g c c c g a g g g c

2640 (in frame with hmKO) RTMTQKDVEDMFSRFGRIIN (in frame with mKO2) RTMTQKDVEDMFSRFGRIIN RTMTQKDVEDMFSRFGRIIN c g g a c c a t g a c c c a g a a g g a c g t a g a a g a c a t g t t c t c t c g g t t t g g g c g g a t c a t c a a c g c c t g g t a c t g g g t c t t c c t g c a t c t t c t g t a c a a g a g a g c c a a a c c c g c c t a g t a g t t g

2700 (in frame with hmKO) SRVLVDQTTGLSRGVAFIRF (in frame with mKO2) SRVLVDQTTGLSRGVAFIRF SRVLVDQTTGLSRGVAFIRF t c g c g g g t c c t c g t g g a t c a g a c t a c a g g t t t g t c c a g a g g g g t t g c g t t t a t c c g g t t t a g c g c c c a g g a g c a c c t a g t c t g a t g t c c a a a c a g g t c t c c c c a a c g c a a a t a g g c c a a a

2760 (in frame with hmKO) DKRSEAEEAITSFNGHKPPG (in frame with mKO2) DKRSEAEEAITSFNGHKPPG DKRSEAEEAITSFNGHKPPG g a c a a a c g g t c g g a g g c a g a a g a g g c a a t t a c c a g t t t c a a t g g t c a t a a a c c c c c a g g t c t g t t t g c c a g c c t c c g t c t t c t c c g t t a a t g g t c a a a g t t a c c a g t a t t t g g g g g t c c a

2820 (in frame with hmKO) SSEPITVKFAANPNQNKNVA (in frame with mKO2) SSEPITVKFAANPNQNKNVA SSEPITVKFAANPNQNKNVA t c c t c t g a g c c c a t c a c a g t g a a g t t t g c a g c c a a c c c c a a c c a g a a c a a a a a c g t g g c a a g g a g a c t c g g g t a g t g t c a c t t c a a a c g t c g g t t g g g g t t g g t c t t g t t t t t g c a c c g t

2880 (in frame with hmKO) LLSQLYHSPARRFGGPVHHQ (in frame with mKO2) LLSQLYHSPARRFGGPVHHQ LLSQLYHSPARRFGGPVHHQ c t c c t c t c g c a g c t g t a c c a c t c g c c a g c g c g a c g g t t c g g a g g c c c c g t t c a c c a c c a g g a g g a g a g c g t c g a c a t g g t g a g c g g t c g c g c t g c c a a g c c t c c g g g g c a a g t g g t g g t c

2940 (in frame with hmKO) AQRFRFDPMGVDHMSGLSGV (in frame with mKO2) AQRFRFDPMGVDHMSGLSGV AQRFRFDPMGVDHMSGLSGV g c g c a g a g a t t c a g g t t c g a c c c c a t g g g c g t c g a t c a c a t g a g c g g g c t c t c t g g c g t c c g c g t c t c t a a g t c c a a g c t g g g g t a c c c g c a g c t a g t g t a c t c g c c c g a g a g a c c g c a g

3000 (in frame with hmKO) NVSGNASSGWCIFIYNLGQD (in frame with mKO2) NVSGNASSGWCIFIYNLGQD NVSGNASSGWCIFIYNLGQD a a c g t g t c a g g a a a c g c c t c c t c c g g c t g g t g c a t t t t c a t c t a c a a c c t g g g g c a g g a t t t g c a c a g t c c t t t g c g g a g g a g g c c g a c c a c g t a a a a g t a g a t g t t g g a c c c c g t c c t a

BamHI 3060 (in frame with hmKO) ADEGILWQMFGPFGAVTNVK (in frame with mKO2) ADEGILWQMFGPFGAVTNVK ADEGILWQMFGPFGAVTNVK g c c g a c g a g g g g a t c c t c t g g c a g a t g t t t g g g c c g t t t g g t g c c g t c a c c a a t g t g a a a c g g c t g c t c c c c t a g g a g a c c g t c t a c a a a c c c g g c a a a c c a c g g c a g t g g t t a c a c t t t

3120 (in frame with hmKO) VIRDFNTNKCKGFGFVTMTN (in frame with mKO2) VIRDFNTNKCKGFGFVTMTN VIRDFNTNKCKGFGFVTMTN g t g a t c c g c g a c t t c a a c a c c a a c a a g t g c a a a g g g t t t g g c t t t g t g a c c a t g a c a a a c c a c t a g g c g c t g a a g t t g t g g t t g t t c a c g t t t c c c a a a c c g a a a c a c t g g t a c t g t t t g

BtgZI 3180 (in frame with hmKO) YEEAAMAIASLNGYRLGDKI (in frame with mKO2) YEEAAMAIASLNGYRLGDKI YEEAAMAIASLNGYRLGDKI t a t g a a g a a g c c g c g a t g g c c a t a g c c a g c c t g a a c g g c t a c c g c c t g g g g g a c a a a a t c a t a c t t c t t c g g c g c t a c c g g t a t c g g t c g g a c t t g c c g a t g g c g g a c c c c c t g t t t t a g

3240 (in frame with hmKO) LQVSFKTNKSHK* SV40 promoter (in frame with mKO2) LQVSFKTNKSHK* C C T T A C A C A C pBABE 3' LQVSFKTNKSHK* t t a c a g g t t t c c t t c a a a a c c a a c a a g t c c c a c a a a t a a c t c g a c c c t g t g g a a t g t g t g a a t g t c c a a a g g a a g t t t t g g t t g t t c a g g g t g t t t a t t g a g c t g g g a c a c c t t a c a c a c

3300 SV40 promoter A G T C A A T C C C A pBABE 3' t c a g t t a g g g t g t g g a a a g t c c c c a g g c t c c c c a g c a g g c a g a a g t a t g c a a a g c a t g c a a g t c a a t c c c a c a c c t t t c a g g g g t c c g a g g g g t c g t c c g t c t t c a t a c g t t t c g t a c g t

3360 SV40 promoter t c t c a a t t a g t c a g c a a c c a g g t g t g g a a a g t c c c c a g g c t c c c c a g c a g g c a g a a g t a t a g a g t t a a t c a g t c g t t g g t c c a c a c c t t t c a g g g g t c c g a g g g g t c g t c c g t c t t c a t a

3420 SV40 promoter SV40 ori g c a a a g c a t g c a t c t c a a t t a g t c a g c a a c c a t a g t c c c g c c c c t a a c t c c g c c c a t c c c c g t t t c g t a c g t a g a g t t a a t c a g t c g t t g g t a t c a g g g c g g g g a t t g a g g c g g g t a g g g

3480 SV40 promoter SV40 ori g c c c c t a a c t c c g c c c a g t t c c g c c c a t t c t c c g c c c c a t g g c t g a c t a a t t t t t t t t a t c g g g g a t t g a g g c g g g t c a a g g c g g g t a a g a g g c g g g g t a c c g a c t g a t t a a a a a a a a t a

SfiI 3540 SV40 promoter SV40 ori t t a t g c a g a g g c c g a g g c c g c c t c g g c c t c t g a g c t a t t c c a g a a g t a g t g a g g a g g c t t a a t a c g t c t c c g g c t c c g g c g g a g c c g g a g a c t c g a t a a g g t c t t c a t c a c t c c t c c g a a

AvrII 3600 1 5 MTEYKPTVR SV40 promoter PuroR SV40 ori T A C T G G C T C A T G T T C G G G T G Puro-R MTEYKPTVR *AKAFLKGHGLVLGRHA t t t t g g a g g c c t a g g c t t t t g c a a a a a g c t t a c c a t g a c c g a g t a c a a g c c c a c g g t g c g a a a a c c t c c g g a t c c g a a a a c g t t t t t c g a a t g g t a c t g g c t c a t g t t c g g g t g c c a c g c

BsiWI 3660 10 15 20 25 LATRDDVPRAVRTLAAAFAD PuroR LATRDDVPRAVRTLAAAFAD EGGAVVDGPGYAGEGGREGV c c t c g c c a c c c g c g a c g a c g t c c c c a g g g c c g t a c g c a c c c t c g c c g c c g c g t t c g c c g a g g a g c g g t g g g c g c t g c t g c a g g g g t c c c g g c a t g c g t g g g a g c g g c g g c g c a a g c g g c t

RsrII 3720 30 35 40 45 YPATRHTVDPDRHIERVTEL PuroR YPATRHTVDPDRHIERVTEL VGGRAVGDIRVAVDLPDGLQ c t a c c c c g c c a c g c g c c a c a c c g t c g a t c c g g a c c g c c a c a t c g a g c g g g t c a c c g a g c t g a t g g g g c g g t g c g c g g t g t g g c a g c t a g g c c t g g c g g t g t a g c t c g c c c a g t g g c t c g a

3780 50 55 60 65 QELFLTRVGLDIGKVWVADD PuroR QELFLTRVGLDIGKVWVADD LFEEERADPEVDALHPDRVV g c a a g a a c t c t t c c t c a c g c g c g t c g g g c t c g a c a t c g g c a a g g t g t g g g t c g c g g a c g a c g t t c t t g a g a a g g a g t g c g c g c a g c c c g a g c t g t a g c c g t t c c a c a c c c a g c g c c t g c t

3840 70 75 80 85 GAAVAVWTTPESVEAGAVFA PuroR GAAVAVWTTPESVEAGAVFA AGRHRDPGRRLADFRPRHEG c g g c g c c g c g g t g g c g g t c t g g a c c a c g c c g g a g a g c g t c g a a g c g g g g g c g g t g t t c g c g c c g c g g c g c c a c c g c c a g a c c t g g t g c g g c c t c t c g c a g c t t c g c c c c c g c c a c a a g c g

3900 90 95 100 105 EIGPRMAELSGSRLAAQQQM PuroR EIGPRMAELSGSRLAAQQQM LDARAHGLQATGPQGRLLLH c g a g a t c g g c c c g c g c a t g g c c g a g t t g a g c g g t t c c c g g c t g g c c g c g c a g c a a c a g a t g c t c t a g c c g g g c g c g t a c c g g c t c a a c t c g c c a a g g g c c g a c c g g c g c g t c g t t g t c t a

3960 110 115 120 125 EGLLAPHRPKEPAWFLATVG PuroR EGLLAPHRPKEPAWFLATVG FAEQRRVPGLLGRPEQGGDA g g a a g g c c t c c t g g c g c c g c a c c g g c c c a a g g a g c c c g c g t g g t t c c t g g c c a c c g t c g g c c t t c c g g a g g a c c g c g g c g t g g c c g g g t t c c t c g g g c g c a c c a a g g a c c g g t g g c a g c c

4020 130 135 140 145 VSPDHQGKGLGSAVVLPGVE PuroR VSPDHQGKGLGSAVVLPGVE DRGVVLALTQAAGDHEGSHL c g t c t c g c c c g a c c a c c a g g g c a a g g g t c t g g g c a g c g c c g t c g t g c t c c c c g g a g t g g a g c a g a g c g g g c t g g t g g t c c c g t t c c c a g a c c c g t c g c g g c a g c a c g a g g g g c c t c a c c t

4080 150 155 160 165 AAERAGVPAFLETSAPRNLP PuroR G C A A C C T C C C Puro-F AAERAGVPAFLETSAPRNLP RGLAGPHGGEQLGGRGAVEG g g c g g c c g a g c g c g c c g g g g t g c c c g c c t t c c t g g a g a c c t c c g c g c c c c g c a a c c t c c c c c g c c g g c t c g c g c g g c c c c a c g g g c g g a a g g a c c t c t g g a g g c g c g g g g c g t t g g a g g g

4140 170 175 180 185 FYERLGFTVTADVEVPEGPR PuroR C T T C T A C G A G C Puro-F FYERLGFTVTADVEVPEGPR EVLPEAEGDGGVDLHGFSRA c t t c t a c g a g c g g c t c g g c t t c a c c g t c a c c g c c g a c g t c g a g g t g c c c g a a g g a c c g c g g a a g a t g c t c g c c g a g c c g a a g t g g c a g t g g c g g c t g c a g c t c c a c g g g c t t c c t g g c g c

DraIII 4200 190 195 200 TWCMTRKPGA* PuroR TWCMTRKPGA* GPAHGALGTGSARGVVRLAR c a c c t g g t g c a t g a c c c g c a a g c c c g g t g c c t g a c g c c c g c c c c a c g a c c c g c a g c g c c c g t g g a c c a c g t a c t g g g c g t t c g g g c c a c g g a c t g c g g g c g g g g t g c t g g g c g t c g c g g g

BspDIClaI 4260 GFPACSGM g a c c g a a a g g a g c g c a c g a c c c c a t g c a t c g a t a a a a t a a a a g a t t t t a t t t a g t c t c c a c t g g c t t t c c t c g c g t g c t g g g g t a c g t a g c t a t t t t a t t t t c t a a a a t a a a t c a g a g g t

BmtINheI 4320 g a a a a a g g g g g g a a t g a a a g a c c c c a c c t g t a g g t t t g g c a a g c t a g c t t a a g t a a c g c c c t t t t t c c c c c c t t a c t t t c t g g g g t g g a c a t c c a a a c c g t t c g a t c g a a t t c a t t g c g g

4380 a t t t t g c a a g g c a t g g a a a a a t a c a t a a c t g a g a a t a g a g a a g t t c a g a t c a a g g t c a g g t a a a a c g t t c c g t a c c t t t t t a t g t a t t g a c t c t t a t c t c t t c a a g t c t a g t t c c a g t c c

4440 a a c a g a t g g a a c a g c t g a a t a t g g g c c a a a c a g g a t a t c t g t g g t a a g c a g t t c c t g c c c t t g t c t a c c t t g t c g a c t t a t a c c c g g t t t g t c c t a t a g a c a c c a t t c g t c a a g g a c g g g

4500 c g g c t c a g g g c c a a g a a c a g a t g g a a c a g c t g a a t a t g g g c c a a a c a g g a t a t c t g t g g t g c c g a g t c c c g g t t c t t g t c t a c c t t g t c g a c t t a t a c c c g g t t t g t c c t a t a g a c a c c a

4560 a a g c a g t t c c t g c c c c g g c t c a g g g c c a a g a a c a g a t g g t c c c c a g a t g c g g t c c a g c c c t t c g t c a a g g a c g g g g c c g a g t c c c g g t t c t t g t c t a c c a g g g g t c t a c g c c a g g t c g g g

4620 t c a g c a g t t t c t a g a g a a c c a t c a g a t g t t t c c a g g g t g c c c c a a g g a c c t g a a a t g a c c a g t c g t c a a a g a t c t c t t g g t a g t c t a c a a a g g t c c c a c g g g g t t c c t g g a c t t t a c t g g

4680 c t g t g c c t t a t t t g a a c t a a c c a a t c a g t t c g c t t c t c g c t t c t g t t c g c g c g c t t c t g c g a c a c g g a a t a a a c t t g a t t g g t t a g t c a a g c g a a g a g c g a a g a c a a g c g c g c g a a g a c g

4740 t c c c c g a g c t c a a t a a a a g a g c c c a c a a c c c c t c a c t c g g g g c g c c a g t c c t c c g a t t g a a g g g g c t c g a g t t a t t t t c t c g g g t g t t g g g g a g t g a g c c c c g c g g t c a g g a g g c t a a c t

4800 c t g a g t c g c c c g g g t a c c c g t g t a t c c a a t a a a c c c t c t t g c a g t t g c a t c c g a c t t g t g g a c t c a g c g g g c c c a t g g g c a c a t a g g t t a t t t g g g a g a a c g t c a a c g t a g g c t g a a c a c

4860 g t c t c g c t g t t c c t t g g g a g g g t c t c c t c t g a g t g a t t g a c t a c c c g t c a g c g g g g g g c a c a g a g c g a c a a g g a a c c c t c c c a g a g g a g a c t c a c t a a c t g a t g g g c a g t c g c c c c c c g t

4920 g t t g c a t c c g a c t t g t g g t c t c g c t g t t c c t t g g g a g g g t c t c c t c t g a g t g a t t g a c t a c a a c g t a g g c t g a a c a c c a g a g c g a c a a g g a a c c c t c c c a g a g g a g a c t c a c t a a c t g a t

4980 c c c g t c a g c g g g g g t c t t t c a c a t g c a g c a t g t a t c a a a a t t a a t t t g g t t t t t t t t c t t g g g c a g t c g c c c c c a g a a a g t g t a c g t c g t a c a t a g t t t t a a t t a a a c c a a a a a a a a g a a

5040 a a g t a t t t a c a t t a a a t g g c c a t a g t t g c a t t a a t g a a t c g g c c a a c g c g c g g g g a g a g g t t c a t a a a t g t a a t t t a c c g g t a t c a a c g t a a t t a c t t a g c c g g t t g c g c g c c c c t c t c c

BspQISapI 5100 G A G C G A G T G A C T G A G C G A L4440 c g g t t t g c g t a t t g g g c g c t c t t c c g c t t c c t c g c t c a c t g a c t c g c t g c g c t c g g t c g t g c c a a a c g c a t a a c c c g c g a g a a g g c g a a g g a g c g a g t g a c t g a g c g a c g c g a g c c a g c a

5160 t c g g c t g c g g c g a g c g g t a t c a g c t c a c t c a a a g g c g g t a a t a c g g t t a t c c a c a g a a t c a g c c g a c g c c g c t c g c c a t a g t c g a g t g a g t t t c c g c c a t t a t g c c a a t a g g t g t c t t a g

5220 a g g g g a t a a c g c a g g a a a g a a c a t g t g a g c a a a a g g c c a g c a a a a g g c c a g g a a c c g t a a t c c c c t a t t g c g t c c t t t c t t g t a c a c t c g t t t t c c g g t c g t t t t c c g g t c c t t g g c a t t

5280 ori a a a g g c c g c g t t g c t g g c g t t t t t c c a t a g g c t c c g c c c c c c t g a c g a g c a t c a c a a a a a t t t c c g g c g c a a c g a c c g c a a a a a g g t a t c c g a g g c g g g g g g a c t g c t c g t a g t g t t t t t

5340 ori T T T C T A T G G T C C G C A A A G G pBR322ori-F t c g a c g c t c a a g t c a g a g g t g g c g a a a c c c g a c a g g a c t a t a a a g a t a c c a g g c g t t t c c a g c t g c g a g t t c a g t c t c c a c c g c t t t g g g c t g t c c t g a t a t t t c t a t g g t c c g c a a a g g

5400 ori G pBR322ori-F c c c t g g a a g c t c c c t c g t g c g c t c t c c t g t t c c g a c c c t g c c g c t t a c c g g a t a c c t g t c g g g a c c t t c g a g g g a g c a c g c g a g a g g a c a a g g c t g g g a c g g c g a a t g g c c t a t g g a c a g

5460 ori c g c c t t t c t c c c t t c g g g a a g c g t g g c g c t t t c t c a t a g c t c a c g c t g t a g g t a t c t c a g g c g g a a a g a g g g a a g c c c t t c g c a c c g c g a a a g a g t a t c g a g t g c g a c a t c c a t a g a g t c

5520 ori t t c g g t g t a g g t c g t t c g c t c c a a g c t g g g c t g t g t g c a c g a a c c c c c c g t t c a g c c c g a a a g c c a c a t c c a g c a a g c g a g g t t c g a c c c g a c a c a c g t g c t t g g g g g g c a a g t c g g g c t

5580 ori c c g c t g c g c c t t a t c c g g t a a c t a t c g t c t t g a g t c c a a c c c g g t a a g a c a c g a c t t a t c g g c g a c g c g g a a t a g g c c a t t g a t a g c a g a a c t c a g g t t g g g c c a t t c t g t g c t g a a t a g

5640 ori g c c a c t g g c a g c a g c c a c t g g t a a c a g g a t t a g c a g a g c g a g g t a t g t a g g c g g t g c t a c c g g t g a c c g t c g t c g g t g a c c a t t g t c c t a a t c g t c t c g c t c c a t a c a t c c g c c a c g a t g

5700 ori a g a g t t c t t g a a g t g g t g g c c t a a c t a c g g c t a c a c t a g a a g a a c a g t a t t t g g t a t c t g t c t c a a g a a c t t c a c c a c c g g a t t g a t g c c g a t g t g a t c t t c t t g t c a t a a a c c a t a g a c

5760 ori c g c t c t g c t g a a g c c a g t t a c c t t c g g a a a a a g a g t t g g t a g c t c t t g a t c c g g c a a a c a g c g a g a c g a c t t c g g t c a a t g g a a g c c t t t t t c t c a a c c a t c g a g a a c t a g g c c g t t t g t

5820 ori a a c c a c c g c t g g t a g c g g t g g t t t t t t t g t t t g c a a g c a g c a g a t t a c g c g c a g a a a a a a t t g g t g g c g a c c a t c g c c a c c a a a a a a a c a a a c g t t c g t c g t c t a a t g c g c g t c t t t t t t

5880 ori a g g a t c t c a a g a a g a t c c t t t g a t c t t t t c t a c g g g g t c t g a c g c t c a g t g g a a c g a a a a t c c t a g a g t t c t t c t a g g a a a c t a g a a a a g a t g c c c c a g a c t g c g a g t c a c c t t g c t t t t

5940 c t c a c g t t a a g g g a t t t t g g t c a t g a g a t t a t c a a a a a g g a t c t t c a c c t a g a t c c t t t t g a g t g c a a t t c c c t a a a a c c a g t a c t c t a a t a g t t t t t c c t a g a a g t g g a t c t a g g a a a a

NotI 6000 285 *WHK AmpR *WHK g c g g c c g c a a a t c a a t c t a a a g t a t a t a t g a g t a a a c t t g g t c t g a c a g t t a c c a a t g c t c g c c g g c g t t t a g t t a g a t t t c a t a t a t a c t c a t t t g a a c c a g a c t g t c a a t g g t t a c g a

6060 280 275 270 265 ILSAGIEAIQRNREDMTAQS AmpR ILSAGIEAIQRNREDMTAQS t a a t c a g t g a g g c a c c t a t c t c a g c g a t c t g t c t a t t t c g t t c a t c c a t a g t t g c c t g a c a t t a g t c a c t c c g t g g a t a g a g t c g c t a g a c a g a t a a a g c a a g t a g g t a t c a a c g g a c t g

6120 260 255 250 245 GTTYIVVIRSPKGDPGLAAI AmpR GTTYIVVIRSPKGDPGLAAI t c c c c g t c g t g t a g a t a a c t a c g a t a c g g g a g g g c t t a c c a t c t g g c c c c a g t g c t g c a a a g g g g c a g c a c a t c t a t t g a t g c t a t g c c c t c c c g a a t g g t a g a c c g g g g t c a c g a c g t t

6180 240 235 230 225 IGRSGREGAGSKDAIFWGAP AmpR IGRSGREGAGSKDAIFWGAP t g a t a c c g c g a g a c c c a c g c t c a c c g g c t c c a g a t t t a t c a g c a a t a a a c c a g c c a g c c g a c t a t g g c g c t c t g g g t g c g a g t g g c c g a g g t c t a a a t a g t c g t t a t t t g g t c g g t c g g c

6240 220 215 210 205 LASRLLPGAVKDAEMWDILQ AmpR LASRLLPGAVKDAEMWDILQ g a a g g g c c g a g c g c a g a a g t g g t c c t g c a a c t t t a t c c g c c t c c a t c c a g t c t a t t a a t t c t t c c c g g c t c g c g t c t t c a c c a g g a c g t t g a a a t a g g c g g a g g t a g g t c a g a t a a t t a a

FspI 6300 200 195 190 185 QRSALTLLEGTLLKRLTTAM AmpR QRSALTLLEGTLLKRLTTAM g t t g c c g g g a a g c t a g a g t a a g t a g t t c g c c a g t t a a t a g t t t g c g c a a c g t t g t t g c c a c a a c g g c c c t t c g a t c t c a t t c a t c a a g c g g t c a a t t a t c a a a c g c g t t g c a a c a a c g g t

6360 180 175 170 165 AVPMTTDREDNPIAENLEPE AmpR AVPMTTDREDNPIAENLEPE t t g c t a c a g g c a t c g t g g t g t c a c g c t c g t c g t t t g g t a t g g c t t c a t t c a g c t c c g g t t a a c g a t g t c c g t a g c a c c a c a g t g c g a g c a g c a a a c c a t a c c g a a g t a a g t c g a g g c c a a

6420 160 155 150 145 WRDLRTVHDGMNHLFATLEK AmpR WRDLRTVHDGMNHLFATLEK c c c a a c g a t c a a g g c g a g t t a c a t g a t c c c c c a t g t t g t g c a a a a a a g c g g t t a g c t c c t g g g t t g c t a g t t c c g c t c a a t g t a c t a g g g g g t a c a a c a c g t t t t t t c g c c a a t c g a g g a

PvuI 6480 140 135 130 125 PGGITTLLLNAATNDSMTIA AmpR PGGITTLLLNAATNDSMTIA t c g g t c c t c c g a t c g t t g t c a g a a g t a a g t t g g c c g c a g t g t t a t c a c t c a t g g t t a t g g a g c c a g g a g g c t a g c a a c a g t c t t c a t t c a a c c g g c g t c a c a a t a g t g a g t a c c a a t a c c

6540 120 115 110 105 ASCLERVTMGDTLHKETVPS AmpR ASCLERVTMGDTLHKETVPS c a g c a c t g c a t a a t t c t c t t a c t g t c a t g c c a t c c g t a a g a t g c t t t t c t g t g a c t g g t g g t c g t g a c g t a t t a a g a g a a t g a c a g t a c g g t a g g c a t t c t a c g a a a a g a c a c t g a c c a c

ScaI 6600 100 95 90 85 YEVLDNQSYHIRRGLQEQGA AmpR YEVLDNQSYHIRRGLQEQGA a g t a c t c a a c c a a g t c a t t c t g a g a a t a g t g t a t g c g g c g a c c g a g t t g c t c t t g c c c g g t c a t g a g t t g g t t c a g t a a g a c t c t t a t c a c a t a c g c c g c t g g c t c a a c g a g a a c g g g c c

6660 80 75 70 65 DIRSLVAGCLLVKFTSMMPF AmpR A T A A T A C C G C G C C A C A T A G C Amp-R DIRSLVAGCLLVKFTSMMPF c g t c a a t a c g g g a t a a t a c c g c g c c a c a t a g c a g a a c t t t a a a a g t g c t c a t c a t t g g a a g c a g t t a t g c c c t a t t a t g g c g c g g t g t a t c g t c t t g a a a t t t t c a c g a g t a g t a a c c t t

XmnI 6720 60 55 50 45 REEPRFSELIKGSNLDLEIY AmpR REEPRFSELIKGSNLDLEIY a a c g t t c t t c g g g g c g a a a a c t c t c a a g g a t c t t a c c g c t g t t g a g a t c c a g t t c g a t g t t t g c a a g a a g c c c c g c t t t t g a g a g t t c c t a g a a t g g c g a c a a c t c t a g g t c a a g c t a c a

6780 40 35 30 25 GVRAGLQDEADKVKVLTEPH AmpR GVRAGLQDEADKVKVLTEPH a a c c c a c t c g t g c a c c c a a c t g a t c t t c a g c a t c t t t t a c t t t c a c c a g c g t t t c t g g g t t t g g g t g a g c a c g t g g g t t g a c t a g a a g t c g t a g a a a a t g a a a g t g g t c g c a a a g a c c c a

6840 20 15 10 5 signal sequence AFVPLCFAAFFPILAVRFHQ AmpR AFVPLCFAAFFPILAVRFHQ g a g c a a a a a c a g g a a g g c a a a a t g c c g c a a a a a a g g g a a t a a g g g c g a c a c g g a a a t g t t c t c g t t t t t g t c c t t c c g t t t t a c g g c g t t t t t t c c c t t a t t c c c g c t g t g c c t t t a c a a

SspI 6900 1 signal sequence ISM AmpR AmpR promoter ISM g a a t a c t c a t a c t c t t c c t t t t t c a a t a t t a t t g a a g c a t t t a t c a g g g t t a t t g t c t c a c t t a t g a g t a t g a g a a g g a a a a a g t t a t a a t a a c t t c g t a a a t a g t c c c a a t a a c a g a g t

6960 AmpR promoter t g a g c g g a t a c a t a t t t g a a t g t a t t t a g a a a a a t a a a c a a a t a g g g g t t c c g c g c a c a t a c t c g c c t a t g t a t a a a c t t a c a t a a a t c t t t t t a t t t g t t t a t c c c c a a g g c g c g t g t a

6978 t t c c c c g a a a a g t g c c a c a a g g g g c t t t t c a c g g t g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.