Addgene PTE_24 Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: PTE_24 Sequencing Result


Map View

PluTI (7357) SfoI (7355) NarI * (7354) KasI (7353) EcoRV (7164) ApaI (6925) PspOMI (6921) BstEII (6895) LacI-R (6383 .. 6402) MscI * (6258) FspAI (6248) Bpu10I (6120) pGEX 3' (5583 .. 5605) PflFI - Tth111I (5483) BstZ17I (5458) AccI (5457) pRS-marker (5423 .. 5442) NdeI (5406) BspQI - SapI (5344) L4440 (5325 .. 5342) PciI (5227) pBR322ori-F (5072 .. 5091) F1ori-R (4366 .. 4385) DraIII (4241) F1ori-F (4154 .. 4175) SwaI (4016) M13/pUC Reverse (91 .. 113) lac operator BsiWI (462) BglII (531) BmgBI (714) BsmI (792) BlpI (958) NcoI - StyI (1133) MBP-F (1246 .. 1269) Eco53kI (1280) SacI (1282) XmnI (1332) EcoRI (1339) NsiI (1421) EagI (1575) NruI (1834) BstBI (1904) StuI (1916) PstI (2363) HindIII (2367) M13 Forward (2376 .. 2393) M13/pUC Forward (2385 .. 2407) pBAD Reverse (2571 .. 2588) Amp-R (3275 .. 3294) ScaI (3363) PTE_24 7639 bp

Sequence View

5ʹ 3ʹ 60 -35 tac promoter t t t t g c g c c g a c a t c a t a a c g g t t c t g g c a a a t a t t c t g a a a t g a g c t g t t g a c a a t t a a a a a a c g c g g c t g t a g t a t t g c c a a g a c c g t t t a t a a g a c t t t a c t c g a c a a c t g t t a a t t

120 -10 tac promoter lac operator A G C G G A T A A C A A T T T C A C A C A G G M13/pUC Reverse t c a t c g g c t c g t a t a a t g t g t g g a a t t g t g a g c g g a t a a c a a t t t c a c a c a g g a a a c a g c a g t a g c c g a g c a t a t t a c a c a c c t t a a c a c t c g c c t a t t g t t a a a g t g t g t c c t t t g t c g

180 1 MKT MBP MKT c a g t c c g t t t a g g t g t t t t c a c g a g c a c t t c a c c a a c a a g g a c c a t a g a t t a t g a a a a c t g t c a g g c a a a t c c a c a a a a g t g c t c g t g a a g t g g t t g t t c c t g g t a t c t a a t a c t t t t g a

240 5 10 15 20 EEGKLVIWINGDKGYNGLAE MBP EEGKLVIWINGDKGYNGLAE g a a g a a g g t a a a c t g g t a a t c t g g a t t a a c g g c g a t a a a g g c t a t a a c g g t c t c g c t g a a c t t c t t c c a t t t g a c c a t t a g a c c t a a t t g c c g c t a t t t c c g a t a t t g c c a g a g c g a c t t

300 25 30 35 40 VGKKFEKDTGIKVTVEHPDK MBP VGKKFEKDTGIKVTVEHPDK g t c g g t a a g a a a t t c g a g a a a g a t a c c g g a a t t a a a g t c a c c g t t g a g c a t c c g g a t a a a c a g c c a t t c t t t a a g c t c t t t c t a t g g c c t t a a t t t c a g t g g c a a c t c g t a g g c c t a t t t

360 45 50 55 60 LEEKFPQVAATGDGPDIIFW MBP LEEKFPQVAATGDGPDIIFW c t g g a a g a g a a a t t c c c a c a g g t t g c g g c a a c t g g c g a t g g c c c t g a c a t t a t c t t c t g g g a c c t t c t c t t t a a g g g t g t c c a a c g c c g t t g a c c g c t a c c g g g a c t g t a a t a g a a g a c c

420 65 70 75 80 AHDRFGGYAQSGLLAEITPD MBP AHDRFGGYAQSGLLAEITPD g c a c a c g a c c g c t t t g g t g g c t a c g c t c a a t c t g g c c t g t t g g c t g a a a t c a c c c c g g a c c g t g t g c t g g c g a a a c c a c c g a t g c g a g t t a g a c c g g a c a a c c g a c t t t a g t g g g g c c t g

BsiWI 480 85 90 95 100 KAFQDKLYPFTWDAVRYNGK MBP KAFQDKLYPFTWDAVRYNGK a a a g c g t t c c a g g a c a a g c t g t a t c c g t t t a c c t g g g a t g c c g t a c g t t a c a a c g g c a a g t t t c g c a a g g t c c t g t t c g a c a t a g g c a a a t g g a c c c t a c g g c a t g c a a t g t t g c c g t t c

BglII 540 105 110 115 120 LIAYPIAVEALSLIYNKDLL MBP LIAYPIAVEALSLIYNKDLL c t g a t t g c t t a c c c g a t c g c t g t t g a a g c g t t a t c g c t g a t t t a t a a c a a a g a t c t g c t g g a c t a a c g a a t g g g c t a g c g a c a a c t t c g c a a t a g c g a c t a a a t a t t g t t t c t a g a c g a c

600 125 130 135 140 PNPPKTWEEIPALDKELKAK MBP PNPPKTWEEIPALDKELKAK c c g a a c c c g c c a a a a a c c t g g g a a g a g a t c c c g g c g c t g g a t a a a g a a c t g a a a g c g a a a g g c t t g g g c g g t t t t t g g a c c c t t c t c t a g g g c c g c g a c c t a t t t c t t g a c t t t c g c t t t

660 145 150 155 160 GKSALMFNLQEPYFTWPLIA MBP GKSALMFNLQEPYFTWPLIA g g t a a g a g c g c g c t g a t g t t c a a c c t g c a a g a a c c g t a c t t c a c c t g g c c g c t g a t t g c t c c a t t c t c g c g c g a c t a c a a g t t g g a c g t t c t t g g c a t g a a g t g g a c c g g c g a c t a a c g a

BmgBI 720 165 170 175 180 ADGGYAFKYENGKYDIKDVG MBP ADGGYAFKYENGKYDIKDVG g c t g a c g g g g g t t a t g c g t t c a a g t a t g a a a a c g g c a a g t a c g a c a t t a a a g a c g t g g g c c g a c t g c c c c c a a t a c g c a a g t t c a t a c t t t t g c c g t t c a t g c t g t a a t t t c t g c a c c c g

780 185 190 195 200 VDNAGAKAGLTFLVDLIKNK MBP VDNAGAKAGLTFLVDLIKNK g t g g a t a a c g c t g g c g c g a a a g c g g g t c t g a c c t t c c t g g t t g a c c t g a t t a a a a a c a a a c a c c t a t t g c g a c c g c g c t t t c g c c c a g a c t g g a a g g a c c a a c t g g a c t a a t t t t t g t t t

BsmI 840 205 210 215 220 HMNADTDYSIAEAAFNKGET MBP HMNADTDYSIAEAAFNKGET c a c a t g a a t g c a g a c a c c g a t t a c t c c a t c g c a g a a g c t g c c t t t a a t a a a g g c g a a a c a g t g t a c t t a c g t c t g t g g c t a a t g a g g t a g c g t c t t c g a c g g a a a t t a t t t c c g c t t t g t

900 225 230 235 240 AMTINGPWAWSNIDTSKVNY MBP AMTINGPWAWSNIDTSKVNY g c g a t g a c c a t c a a c g g c c c g t g g g c a t g g t c c a a c a t c g a c a c c a g c a a a g t g a a t t a t c g c t a c t g g t a g t t g c c g g g c a c c c g t a c c a g g t t g t a g c t g t g g t c g t t t c a c t t a a t a

BlpI 960 245 250 255 260 GVTVLPTFKGQPSKPFVGVL MBP GVTVLPTFKGQPSKPFVGVL g g t g t a a c g g t a c t g c c g a c c t t c a a g g g t c a a c c a t c c a a a c c g t t c g t t g g c g t g c t g c c a c a t t g c c a t g a c g g c t g g a a g t t c c c a g t t g g t a g g t t t g g c a a g c a a c c g c a c g a c

1020 265 270 275 280 SAGINAASPNKELAKEFLEN MBP SAGINAASPNKELAKEFLEN a g c g c a g g t a t t a a c g c c g c c a g t c c g a a c a a a g a g c t g g c a a a a g a g t t c c t c g a a a a c t c g c g t c c a t a a t t g c g g c g g t c a g g c t t g t t t c t c g a c c g t t t t c t c a a g g a g c t t t t g

1080 285 290 295 300 YLLTDEGLEAVNKDKPLGAV MBP YLLTDEGLEAVNKDKPLGAV t a t c t g c t g a c t g a t g a a g g t c t g g a a g c g g t t a a t a a a g a c a a a c c g c t g g g t g c c g t a a t a g a c g a c t g a c t a c t t c c a g a c c t t c g c c a a t t a t t t c t g t t t g g c g a c c c a c g g c a t

NcoIStyI 1140 305 310 315 320 ALKSYEEELAKDPRIAATME MBP Kozak sequence ALKSYEEELAKDPRIAATME g c g c t g a a g t c t t a c g a g g a a g a g t t g g c g a a a g a t c c a c g t a t t g c c g c c a c c a t g g a a c g c g a c t t c a g a a t g c t c c t t c t c a a c c g c t t t c t a g g t g c a t a a c g g c g g t g g t a c c t t

1200 325 330 335 340 NAQKGEIMPNIPQMSAFWYA MBP NAQKGEIMPNIPQMSAFWYA a a c g c c c a g a a a g g t g a a a t c a t g c c g a a c a t c c c g c a g a t g t c c g c t t t c t g g t a t g c c t t g c g g g t c t t t c c a c t t t a g t a c g g c t t g t a g g g c g t c t a c a g g c g a a a g a c c a t a c g g

1260 345 350 355 360 VRTAVINAASGRQTVDEALK MBP G A T G A A G C C C T G A A A MBP-F VRTAVINAASGRQTVDEALK g t g c g t a c t g c g g t g a t c a a c g c c g c c a g c g g t c g t c a g a c t g t c g a t g a a g c c c t g a a a c a c g c a t g a c g c c a c t a g t t g c g g c g g t c g c c a g c a g t c t g a c a g c t a c t t c g g g a c t t t

Eco53kISacI 1320 365 DAQT (in frame with MBP) NSSSNNNNNNNNNNLG MBP G A C G C G C A G MBP-F DAQTNSSSNNNNNNNNNNLG g a c g c g c a g a c t a a t t c g a g c t c g a a c a a c a a c a a c a a t a a c a a t a a c a a c a a c c t c g g g c t g c g c g t c t g a t t a a g c t c g a g c t t g t t g t t g t t g t t a t t g t t a t t g t t g t t g g a g c c c

EcoRIXmnI 1380 1 IEGR (in frame with Factor Xa site) ISEFITNSGDRINTVR Factor Xa site IEGRISEFITNSGDRINTVR *FEDGVAAITDVGNT a t c g a g g g a a g g a t t t c a g a a t t c a t c a c c a a c a g c g g c g a t c g t a t c a a c a c c g t t c g t t a g c t c c c t t c c t a a a g t c t t a a g t a g t g g t t g t c g c c g c t a g c a t a g t t g t g g c a a g c a

NsiI 1440 (in frame with Factor Xa site) GPITISEAGFTLMHEHICGS GPITISEAGFTLMHEHICGS MSISAVL TRDGDRLGTESQHMLMDATR g g t c c g a t c a c c a t c t c t g a g g c c g g t t t c a c t c t g a t g c a t g a g c a t a t c t g c g g t t c t c c a g g c t a g t g g t a g a g a c t c c g g c c a a a g t g a g a c t a c g t a c t c g t a t a g a c g c c a a g a

1500 (in frame with Factor Xa site) SAGFLRAWPEFFGSRDALAE SAGFLRAWPEFFGSRDALAE LRVFSALGRSSSVPVMHWRK RRTKEASPRLEETGTICQRF t c t g c g g g t t t t c t c c g c g c t t g g c c g g a g t t c t t c g g t t c c c g t g a t g c a c t g g c g g a a a g a c g c c c a a a a g a g g c g c g a a c c g g c c t c a a g a a g c c a a g g g c a c t a c g t g a c c g c c t t

1560 (in frame with Factor Xa site) KAVRGLRRARAAGVRTIVDV KAVRGLRRARAAGVRTIVDV KRFAASVALVLPAFVPSLMF FRNAAETASTSGANTGDNIN a a a g c g g t t c g c g g c c t c c g t c g c g c t c g t g c t g c c g g c g t t c g t a c c a t c g t t g a t g t t t t t c g c c a a g c g c c g g a g g c a g c g c g a g c a c g a c g g c c g c a a g c a t g g t a g c a a c t a c a a

EagI 1620 (in frame with Factor Xa site) STFDLGRDVELLAEVSEAAD STFDLGRDVELLAEVSEAAD LPLISAVTLSCSPKFLKRRT RGKIEATVNLQEGFNRFRRV t c t a c c t t t g a t c t c g g c c g t g a c g t t g a g c t g c t c g c c g a a g t t t c t g a a g c g g c g g a c a g a t g g a a a c t a g a g c c g g c a c t g c a a c t c g a c g a g c g g c t t c a a a g a c t t c g c c g c c t g

1680 (in frame with Factor Xa site) VHIVAATGLWFDPPLSMRLR VHIVAATGLWFDPPLSMRLR FTSSRRRVCGSTRLCLCACV NVDDRRRTQPEVRRQRHAQT g t t c a c a t c g t c g c g g c g a c g g g t c t g t g g t t c g a c c c g c c t c t g t c t a t g c g c c t g c g t c a a g t g t a g c a g c g c c g c t g c c c a g a c a c c a a g c t g g g c g g a g a c a g a t a c g c g g a c g c a

1740 (in frame with Factor Xa site) SVEELTQFFLREIQYGIEDT SVEELTQFFLREIQYGIEDT LLRNSPSSFCAKSSTVSRIP RNLFEGLEKQAFDLVTDLIG t c t g t t g a g g a a c t c a c c c a g t t c t t t c t g c g c g a a a t c c a g t a c g g t a t c g a g g a t a c c a g a c a a c t c c t t g a g t g g g t c a a g a a a g a c g c g c t t t a g g t c a t g c c a t a g c t c c t a t g g

1800 (in frame with Factor Xa site) GIRAGIIKVATTGKATPFQE GIRAGIIKVATTGKATPFQE ASVQVSSKLRPPVKPRRSRS ADTCTDDFNRGGTFGRRELL g g c a t c c g t g c a g g t a t c a t c a a a g t t g c g a c c a c c g g t a a a g c c a c g c c g t t c c a g g a g c c g t a g g c a c g t c c a t a g t a g t t t c a a c g c t g g t g g c c a t t t c g g t g c g g c a a g g t c c t c

NruI 1860 (in frame with Factor Xa site) RVLRAAARASLATGVPVTTH RVLRAAARASLATGVPVTTH VYSVRLHAPLSRPVCLLPPI TYETRSCAGRERGTHRNGGM c g t g t a c t c c g t g c g g c t g c a c g c g c c t c t c t c g c g a c c g g t g t g c c t g t t a c c a c c c a t g c a c a t g a g g c a c g c c g a c g t g c g c g g a g a g a g c g c t g g c c a c a c g g a c a a t g g t g g g t a

StuIBstBI 1920 (in frame with Factor Xa site) TDASQRDGEQQADIFESEGL TDASQRDGEQQADIFESEGL PMLLSVTVSSRRTSSNLKAS GISRLTVTLLLRVDEFRFAE a c c g a t g c t t c t c a g c g t g a c g g t g a g c a g c a g g c g g a c a t c t t c g a a t c t g a a g g c c t c t g g c t a c g a a g a g t c g c a c t g c c a c t c g t c g t c c g c c t g t a g a a g c t t a g a c t t c c g g a g

1980 (in frame with Factor Xa site) DPSRVCIGHSDDTDDLDYLT DPSRVCIGHSDDTDDLDYLT IRAVFALVILTTPTTWTT* IRATNANTM g a t c c g a g c c g t g t t t g c a t t g g t c a t t c t g a c g a c a c c g a c g a c c t g g a c t a c c t g a c c c t a g g c t c g g c a c a a a c g t a a c c a g t a a g a c t g c t g t g g c t g c t g g a c c t g a t g g a c t g g

2040 (in frame with Factor Xa site) ALAARGYLIGLDHIPWSAIG ALAARGYLIGLDHIPWSAIG g c t c t c g c a g c t c g c g g t t a t c t g a t t g g t c t g g a t c a c a t t c c g t g g a g c g c g a t c g g c c g a g a g c g t c g a g c g c c a a t a g a c t a a c c a g a c c t a g t g t a a g g c a c c t c g c g c t a g c c g

2100 (in frame with Factor Xa site) LEDNASAAAILGLRSWQTRA LEDNASAAAILGLRSWQTRA c t g g a a g a c a a c g c g t c c g c t g c c g c g a t c c t g g g c c t g c g c t c t t g g c a a a c c c g t g c c g a c c t t c t g t t g c g c a g g c g a c g g c g c t a g g a c c c g g a c g c g a g a a c c g t t t g g g c a c g g

2160 (in frame with Factor Xa site) LLIKALIDQGYADQILVSND LLIKALIDQGYADQILVSND c t c c t g a t c a a a g c g c t g a t c g a c c a g g g c t a c g c c g a c c a g a t c c t g g t t t c t a a c g a c g a g g a c t a g t t t c g c g a c t a g c t g g t c c c g a t g c g g c t g g t c t a g g a c c a a a g a t t g c t g

2220 (in frame with Factor Xa site) WLFGFSSYVTNIMDVLDRVN WLFGFSSYVTNIMDVLDRVN *TVLMMSTRSRTL t g g c t g t t c g g t t t c t c t t c t t a c g t t a c c a a c a t c a t g g a t g t c c t g g a c c g t g t t a a c a c c g a c a a g c c a a a g a g a a g a a t g c a a t g g t t g t a g t a c c t a c a g g a c c t g g c a c a a t t g

2280 (in frame with Factor Xa site) PDGMAFIPLRVIPFLREKGV PDGMAFIPLRVIPFLREKGV GSPIANMGRRTIGNRRSFPT c c g g a t g g t a t g g c g t t c a t c c c t c t g c g t g t t a t t c c a t t c c t c c g t g a a a a a g g t g t t g g c c t a c c a t a c c g c a a g t a g g g a g a c g c a c a a t a a g g t a a g g a g g c a c t t t t t c c a c a a

2340 (in frame with Factor Xa site) PDETLETIMVDNPARFLSPT PDETLETIMVDNPARFLSPT GSSVRSVIMTSLGARKRDGV c c g g a c g a g a c t c t c g a a a c g a t c a t g g t t g a c a a c c c a g c t c g t t t c c t g t c t c c g a c c g g c c t g c t c t g a g a g c t t t g c t a g t a c c a a c t g t t g g g t c g a g c a a a g g a c a g a g g c t g g

HindIIIPstI 2400 LRAS* M13 fwd T G A C C G G C A G C A A A A T G T M13 Forward G C A A A A T G T T G C A G C A M13/pUC Forward LRAS* RRALQYSCALKASATTKCRR c t g c g t g c g a g c t g a t a a c t g c a g g c a a g c t t g g c a c t g g c c g t c g t t t t a c a a c g t c g t g a c g c a c g c t c g a c t a t t g a c g t c c g t t c g a a c c g t g a c c g g c a g c a a a a t g t t g c a g c a

2460 C T G A C C C M13/pUC Forward SQSFGPTVWSLRRAACGGKA g a c t g g g a a a a c c c t g g c g t t a c c c a a c t t a a t c g c c t t g c a g c a c a t c c c c c t t t c g c c c t g a c c c t t t t g g g a c c g c a a t g g g t t g a a t t a g c g g a a c g t c g t g t a g g g g g a a a g c g g

2520 LQRLLSSARVSRGEWCNRLR a g c t g g c g t a a t a g c g a a g a g g c c c g c a c c g a t c g c c c t t c c c a a c a g t t g c g c a g c c t g t c g a c c g c a t t a t c g c t t c t c c g g g c g t g g c t a g c g g g a a g g g t t g t c a a c g c g t c g g a c

2580 G G A C T A T G T C pBAD Reverse FPSHCSPQKPPHSLIKLRIC a a t g g c g a a t g g c a g c t t g g c t g t t t t g g c g g a t g a g a t a a g a t t t t c a g c c t g a t a c a g t t a c c g c t t a c c g t c g a a c c g a c a a a a c c g c c t a c t c t a t t c t a a a a g t c g g a c t a t g t c

2640 T A A T T T A G pBAD Reverse ILDSRLLPRIFCFKGPPLLA a t t a a a t c a g a a c g c a g a a g c g g t c t g a t a a a a c a g a a t t t g c c t g g c g g c a g t a g c g c g t a a t t t a g t c t t g c g t c t t c g c c a g a c t a t t t t g t c t t a a a c g g a c c g c c g t c a t c g c g c

2700 TTGGSGM g t g g t c c c a c c t g a c c c c a t g c c g a a c t c a g a a g t g a a a c g c c g t a g c g c c g a t g g t a g t c a c c a g g g t g g a c t g g g g t a c g g c t t g a g t c t t c a c t t t g c g g c a t c g c g g c t a c c a t c a

2760 rrnB T1 terminator g t g g g g t c t c c c c a t g c g a g a g t a g g g a a c t g c c a g g c a t c a a a t a a a a c g a a a g g c t c a c a c c c c a g a g g g g t a c g c t c t c a t c c c t t g a c g g t c c g t a g t t t a t t t t g c t t t c c g a g t

2820 rrnB T1 terminator g t c g a a a g a c t g g g c c t t t c g t t t t a t c t g t t g t t t g t c g g t g a a c g c t c t c c t g a g t a g c a g c t t t c t g a c c c g g a a a g c a a a a t a g a c a a c a a a c a g c c a c t t g c g a g a g g a c t c a t c

2880 rrnB T1 terminator g a c a a a t c c g c c g g g a g c g g a t t t g a a c g t t g c g a a g c a a c g g c c c g g a g g g t g g c g g g c c t g t t t a g g c g g c c c t c g c c t a a a c t t g c a a c g c t t c g t t g c c g g g c c t c c c a c c g c c c g

2940 rrnB T2 terminator a g g a c g c c c g c c a t a a a c t g c c a g g c a t c a a a t t a a g c a g a a g g c c a t c c t g a c g g a t g g t c c t g c g g g c g g t a t t t g a c g g t c c g t a g t t t a a t t c g t c t t c c g g t a g g a c t g c c t a c c

3000 rrnB T2 terminator AmpR promoter c c t t t t t g c g t t t c t a c a a a c t c t t t t g t t t a t t t t t c t a a a t a c a t t c a a a t a t g t a t c g g a a a a a c g c a a a g a t g t t t g a g a a a a c a a a t a a a a a g a t t t a t g t a a g t t t a t a c a t a g

3060 1 signa... M AmpR promoter AmpR M c g c t c a t g a g a c a a t a a c c c t g a t a a a t g c t t c a a t a a t a t t g a a a a a g g a a g a g t a t g a g c g a g t a c t c t g t t a t t g g g a c t a t t t a c g a a g t t a t t a t a a c t t t t t c c t t c t c a t a c t

3120 5 10 15 20 signal sequence SIQHFRVALIPFFAAFCLPV AmpR SIQHFRVALIPFFAAFCLPV g t a t t c a a c a t t t c c g t g t c g c c c t t a t t c c c t t t t t t g c g g c a t t t t g c c t t c c t g t t t c a t a a g t t g t a a a g g c a c a g c g g g a a t a a g g g a a a a a a c g c c g t a a a a c g g a a g g a c a a a

3180 signal... FA 25 30 35 40 HPETLVKVKDAEDQLGAR AmpR FAHPETLVKVKDAEDQLGAR t t g c t c a c c c a g a a a c g c t g g t g a a a g t a a a a g a t g c t g a a g a t c a g t t g g g t g c a c g a g a a c g a g t g g g t c t t t g c g a c c a c t t t c a t t t t c t a c g a c t t c t a g t c a a c c c a c g t g c t c

3240 45 50 55 60 VGYIELDLNSGKILESFRPE AmpR VGYIELDLNSGKILESFRPE t g g g t t a c a t c g a a c t g g a t c t c a a c a g c g g t a a g a t c c t t g a g a g t t t t c g c c c c g a a g a c c c a a t g t a g c t t g a c c t a g a g t t g t c g c c a t t c t a g g a a c t c t c a a a a g c g g g g c t t c

3300 65 70 75 80 ERFPMMSTFKVLLCGAVLSR AmpR C G A T A C A C C G C G C C A T A A T A Amp-R ERFPMMSTFKVLLCGAVLSR a a c g t t t c c c a a t g a t g a g c a c t t t t a a a g t t c t g c t a t g t g g c g c g g t a t t a t c c c g t g t t g c a a a g g g t t a c t a c t c g t g a a a a t t t c a a g a c g a t a c a c c g c g c c a t a a t a g g g c a c

3360 85 90 95 100 VDAGQEQLGRRIHYSQNDLV AmpR VDAGQEQLGRRIHYSQNDLV t t g a c g c c g g g c a a g a g c a a c t c g g t c g c c g c a t a c a c t a t t c t c a g a a t g a c t t g g t t g a a c t g c g g c c c g t t c t c g t t g a g c c a g c g g c g t a t g t g a t a a g a g t c t t a c t g a a c c a a c

ScaI 3420 105 110 115 120 EYSPVTEKHLTDGMTVRELC AmpR EYSPVTEKHLTDGMTVRELC a g t a c t c a c c a g t c a c a g a a a a g c a t c t t a c g g a t g g c a t g a c a g t a a g a g a a t t a t g c a t c a t g a g t g g t c a g t g t c t t t t c g t a g a a t g c c t a c c g t a c t g t c a t t c t c t t a a t a c g t

3480 125 130 135 140 SAAITMSDNTAANLLLTTIG AmpR SAAITMSDNTAANLLLTTIG g t g c t g c c a t a a c c a t g a g t g a t a a c a c t g c g g c c a a c t t a c t t c t g a c a a c g a t c g g a g c a c g a c g g t a t t g g t a c t c a c t a t t g t g a c g c c g g t t g a a t g a a g a c t g t t g c t a g c c t c

3540 145 150 155 160 GPKELTAFLHNMGDHVTRLD AmpR GPKELTAFLHNMGDHVTRLD g a c c g a a g g a g c t a a c c g c t t t t t t g c a c a a c a t g g g g g a t c a t g t a a c t c g c c t t g a t c c t g g c t t c c t c g a t t g g c g a a a a a a c g t g t t g t a c c c c c t a g t a c a t t g a g c g g a a c t a g

3600 165 170 175 180 RWEPELNEAIPNDERDTTMP AmpR RWEPELNEAIPNDERDTTMP g t t g g g a a c c g g a g c t g a a t g a a g c c a t a c c a a a c g a c g a g c g t g a c a c c a c g a t g c c t g c a a c c c t t g g c c t c g a c t t a c t t c g g t a t g g t t t g c t g c t c g c a c t g t g g t g c t a c g g a c

3660 185 190 195 200 VAMATTLRKLLTGELLTLAS AmpR VAMATTLRKLLTGELLTLAS t a g c a a t g g c a a c a a c g t t g c g c a a a c t a t t a a c t g g c g a a c t a c t t a c t c t a g c t t c c c a t c g t t a c c g t t g t t g c a a c g c g t t t g a t a a t t g a c c g c t t g a t g a a t g a g a t c g a a g g g

3720 205 210 215 220 RQQLIDWMEADKVAGPLLRS AmpR RQQLIDWMEADKVAGPLLRS g g c a a c a a t t a a t a g a c t g g a t g g a g g c g g a t a a a g t t g c a g g a c c a c t t c t g c g c t c g g c c g t t g t t a a t t a t c t g a c c t a c c t c c g c c t a t t t c a a c g t c c t g g t g a a g a c g c g a g c c

3780 225 230 235 240 ALPAGWFIADKSGAGERGSR AmpR ALPAGWFIADKSGAGERGSR c c c t t c c g g c t g g c t g g t t t a t t g c t g a t a a a t c t g g a g c c g g t g a g c g t g g g t c t c g c g g g g a a g g c c g a c c g a c c a a a t a a c g a c t a t t t a g a c c t c g g c c a c t c g c a c c c a g a g c g c

3840 245 250 255 260 GIIAALGPDGKPSRIVVIYT AmpR GIIAALGPDGKPSRIVVIYT g t a t c a t t g c a g c a c t g g g g c c a g a t g g t a a g c c c t c c c g t a t c g t a g t t a t c t a c a c g a c a t a g t a a c g t c g t g a c c c c g g t c t a c c a t t c g g g a g g g c a t a g c a t c a a t a g a t g t g c t

3900 265 270 275 280 TGSQATMDERNRQIAEIGAS AmpR TGSQATMDERNRQIAEIGAS c g g g g a g t c a g g c a a c t a t g g a t g a a c g a a a t a g a c a g a t c g c t g a g a t a g g t g c c t c a c g c c c c t c a g t c c g t t g a t a c c t a c t t g c t t t a t c t g t c t a g c g a c t c t a t c c a c g g a g t g

3960 285 LIKHW* AmpR M13 ori LIKHW* t g a t t a a g c a t t g g t a a c t g t c a g a c c a a g t t t a c t c a t a t a t a c t t t a g a t t g a t t t a c a c t a a t t c g t a a c c a t t g a c a g t c t g g t t c a a a t g a g t a t a t a t g a a a t c t a a c t a a a t g

SwaI 4020 M13 ori c c c g g t t g a t a a t c a g a a a a g c c c c a a a a a c a g g a a g a t t g t a t a a g c a a a t a t t t a a a t g g g c c a a c t a t t a g t c t t t t c g g g g t t t t t g t c c t t c t a a c a t a t t c g t t t a t a a a t t t a

4080 M13 ori t g t a a a c g t t a a t a t t t t g t t a a a a t t c g c g t t a a a t t t t t g t t a a a t c a g c t c a t t t t t a c a t t t g c a a t t a t a a a a c a a t t t t a a g c g c a a t t t a a a a a c a a t t t a g t c g a g t a a a a a

4140 M13 ori t a a c c a a t a g g c c g a a a t c g g c a a a a t c c c t t a t a a a t c a a a a g a a t a g c c c g a g a t a g g a t t g g t t a t c c g g c t t t a g c c g t t t t a g g g a a t a t t t a g t t t t c t t a t c g g g c t c t a t c c

4200 M13 ori G G T C A A A C C T T G T T C T C A G G T G F1ori-F g t t g a g t g t t g t t c c a g t t t g g a a c a a g a g t c c a c t a t t a a a g a a c g t g g a c t c c a a c g t c a a c t c a c a a c a a g g t c a a a c c t t g t t c t c a g g t g a t a a t t t c t t g c a c c t g a g g t t g c a

DraIII 4260 M13 ori c a a a g g g c g a a a a a c c g t c t a t c a g g g c g a t g g c c c a c t a c g t g a a c c a t c a c c c a a a t c g t t t c c c g c t t t t t g g c a g a t a g t c c c g c t a c c g g g t g a t g c a c t t g g t a g t g g g t t t a g

4320 M13 ori a a g t t t t t t g g g g t c g a g g t g c c g t a a a g c a c t a a a t c g g a a c c c t a a a g g g a g c c c c c g t t c a a a a a a c c c c a g c t c c a c g g c a t t t c g t g a t t t a g c c t t g g g a t t t c c c t c g g g g g c

4380 M13 ori A G G G A A G A A A G C G A A F1ori-R a t t t a g a g c t t g a c g g g g a a a g c c g g c g a a c g t g g c g a g a a a g g a a g g g a a g a a a g c g a a t a a a t c t c g a a c t g c c c c t t t c g g c c g c t t g c a c c g c t c t t t c c t t c c c t t c t t t c g c t t

4440 M13 ori A G G A G F1ori-R a g g a g c g g g c g c t a g g g c g c t g g c a a g t g t a g c g g t c a c g c t g c g c g t a a c c a c c a c a c c t c c t c g c c c g c g a t c c c g c g a c c g t t c a c a t c g c c a g t g c g a c g c g c a t t g g t g g t g t g g

4500 M13 ori c g c c g c g c t t a a t g c g c c g c t a c a g g g c g c g t a a a a g g a t c t a g g t g a a g a t c c t t t t t g g c g g c g c g a a t t a c g c g g c g a t g t c c c g c g c a t t t t c c t a g a t c c a c t t c t a g g a a a a a c

4560 a t a a t c t c a t g a c c a a a a t c c c t t a a c g t g a g t t t t c g t t c c a c t g a g c g t c a g a c c c c g t a t t a g a g t a c t g g t t t t a g g g a a t t g c a c t c a a a a g c a a g g t g a c t c g c a g t c t g g g g c

4620 ori t a g a a a a g a t c a a a g g a t c t t c t t g a g a t c c t t t t t t t c t g c g c g t a a t c t g c t g c t t g c a t c t t t t c t a g t t t c c t a g a a g a a c t c t a g g a a a a a a a g a c g c g c a t t a g a c g a c g a a c g

4680 ori a a a c a a a a a a a c c a c c g c t a c c a g c g g t g g t t t g t t t g c c g g a t c a a g a g c t a c c a a c t c t t t g t t t t t t t g g t g g c g a t g g t c g c c a c c a a a c a a a c g g c c t a g t t c t c g a t g g t t g a g

4740 ori t t t t t c c g a a g g t a a c t g g c t t c a g c a g a g c g c a g a t a c c a a a t a c t g t c c t t c t a g t g t a a a a a g g c t t c c a t t g a c c g a a g t c g t c t c g c g t c t a t g g t t t a t g a c a g g a a g a t c a c a

4800 ori a g c c g t a g t t a g g c c a c c a c t t c a a g a a c t c t g t a g c a c c g c c t a c a t a c c t c g c t c t g c t c g g c a t c a a t c c g g t g g t g a a g t t c t t g a g a c a t c g t g g c g g a t g t a t g g a g c g a g a c g

4860 ori t a a t c c t g t t a c c a g t g g c t g c t g c c a g t g g c g a t a a g t c g t g t c t t a c c g g g t t g g a c t a t t a g g a c a a t g g t c a c c g a c g a c g g t c a c c g c t a t t c a g c a c a g a a t g g c c c a a c c t g a

4920 ori c a a g a c g a t a g t t a c c g g a t a a g g c g c a g c g g t c g g g c t g a a c g g g g g g t t c g t g c a c a c g t t c t g c t a t c a a t g g c c t a t t c c g c g t c g c c a g c c c g a c t t g c c c c c c a a g c a c g t g t g

4980 ori a g c c c a g c t t g g a g c g a a c g a c c t a c a c c g a a c t g a g a t a c c t a c a g c g t g a g c t a t g a g t c g g g t c g a a c c t c g c t t g c t g g a t g t g g c t t g a c t c t a t g g a t g t c g c a c t c g a t a c t c

5040 ori a a a g c g c c a c g c t t c c c g a a g g g a g a a a g g c g g a c a g g t a t c c g g t a a g c g g c a g g g t c g t t t c g c g g t g c g a a g g g c t t c c c t c t t t c c g c c t g t c c a t a g g c c a t t c g c c g t c c c a g c

5100 ori G G G A A A C G C C T G G T A T C T T T pBR322ori-F g a a c a g g a g a g c g c a c g a g g g a g c t t c c a g g g g g a a a c g c c t g g t a t c t t t a t a g t c c t g c t t g t c c t c t c g c g t g c t c c c t c g a a g g t c c c c c t t t g c g g a c c a t a g a a a t a t c a g g a c

5160 ori t c g g g t t t c g c c a c c t c t g a c t t g a g c g t c g a t t t t t g t g a t g c t c g t c a g g g g g g c g g a a g c c c a a a g c g g t g g a g a c t g a a c t c g c a g c t a a a a a c a c t a c g a g c a g t c c c c c c g c c t

5220 ori g c c t a t g g a a a a a c g c c a g c a a c g c g g c c t t t t t a c g g t t c c t g g c c t t t t g c t g g c c t t c g g a t a c c t t t t t g c g g t c g t t g c g c c g g a a a a a t g c c a a g g a c c g g a a a a c g a c c g g a a

PciI 5280 t t g c t c a c a t g t t c t t t c c t g c g t t a t c c c c t g a t t c t g t g g a t a a c c g t a t t a c c g c c t a a c g a g t g t a c a a g a a a g g a c g c a a t a g g g g a c t a a g a c a c c t a t t g g c a t a a t g g c g g a

5340 A G C G A G T C A G T G A G C G L4440 t t g a g t g a g c t g a t a c c g c t c g c c g c a g c c g a a c g a c c g a g c g c a g c g a g t c a g t g a g c g a a c t c a c t c g a c t a t g g c g a g c g g c g t c g g c t t g c t g g c t c g c g t c g c t c a g t c a c t c g c

BspQISapI 5400 bom A G L4440 a g g a a g c g g a a g a g c g c c t g a t g c g g t a t t t t c t c c t t a c g c a t c t g t g c g g t a t t t c a c t c c t t c g c c t t c t c g c g g a c t a c g c c a t a a a a g a g g a a t g c g t a g a c a c g c c a t a a a g t g

AccIBstZ17INdeI 5460 bom A T G T T A G A C G A G A C T A C G G C pRS-marker a c c g c a t a t g g t g c a c t c t c a g t a c a a t c t g c t c t g a t g c c g c a t a g t t a a g c c a g t a t a t g g c g t a t a c c a c g t g a g a g t c a t g t t a g a c g a g a c t a c g g c g t a t c a a t t c g g t c a t a t

Tth111IPflFI 5520 bom c a c t c c g c t a t c g c t a c g t g a c t g g g t c a t g g c t g c g c c c c g a c a c c c g c c a a c a c c c g c g t g a g g c g a t a g c g a t g c a c t g a c c c a g t a c c g a c g c g g g g c t g t g g g c g g t t g t g g g c g

5580 t g a c g c g c c c t g a c g g g c t t g t c t g c t c c c g g c a t c c g c t t a c a g a c a a g c t g t g a c c g t a c t g c g c g g g a c t g c c c g a a c a g a c g a g g g c c g t a g g c g a a t g t c t g t t c g a c a c t g g c a

5640 60 55 *LNEGDDGFRALCS rop C C G G G A G C T G C A T G T G T C A G A G G pGEX 3' c t c c g g g a g c t g c a t g t g t c a g a g g t t t t c a c c g t c a t c a c c g a a a c g c g c g a g g c a g c t g a g g c c c t c g a c g t a c a c a g t c t c c a a a a g t g g c a g t a g t g g c t t t g c g c g c t c c g t c g a

5700 50 45 40 35 RYLEDAHDHLSECIDAQEDA rop g c g g t a a a g c t c a t c a g c g t g g t c g t g c a g c g a t t c a c a g a t g t c t g c c t g t t c a t c c g c c g c c a t t t c g a g t a g t c g c a c c a g c a c g t c g c t a a g t g t c t a c a g a c g g a c a a g t a g g c g

5760 30 25 20 15 DLENLKELLTLTQSRIFRAM rop g t c c a g c t c g t t g a g t t t c t c c a g a a g c g t t a a t g t c t g g c t t c t g a t a a a g c g g g c c a t c a g g t c g a g c a a c t c a a a g a g g t c t t c g c a a t t a c a g a c c g a a g a c t a t t t c g c c c g g t a

5820 10 5 1 NLATKEQKTM rop g t t a a g g g c g g t t t t t t c c t g t t t g g t c a c t g a t g c c t c c g t g t a a g g g g g a t t t c t g t t c a a t t c c c g c c a a a a a a g g a c a a a c c a g t g a c t a c g g a g g c a c a t t c c c c c t a a a g a c a a

5880 c a t g g g g g t a a t g a t a c c g a t g a a a c g a g a g a g g a t g c t c a c g a t a c g g g t t a c t g a t g a g t a c c c c c a t t a c t a t g g c t a c t t t g c t c t c t c c t a c g a g t g c t a t g c c c a a t g a c t a c t

5940 t g a a c a t g c c c g g t t a c t g g a a c g t t g t g a g g g t a a a c a a c t g g c g g t a t g g a t g c g g c g a c t t g t a c g g g c c a a t g a c c t t g c a a c a c t c c c a t t t g t t g a c c g c c a t a c c t a c g c c g c

6000 g g a c c a g a g a a a a a t c a c t c a g g g t c a a t g c c a g c g c t t c g t t a a t a c a g a t g t a g g t g t c c t g g t c t c t t t t t a g t g a g t c c c a g t t a c g g t c g c g a a g c a a t t a t g t c t a c a t c c a c a

6060 t c c a c a g g g t a g c c a g c a g c a t c c t g c g a t g c a g a t c c g g a a c a t a a t g g t g c a g g g c g c a g g t g t c c c a t c g g t c g t c g t a g g a c g c t a c g t c t a g g c c t t g t a t t a c c a c g t c c c g c g

6120 t g a c t t c c g c g t t t c c a g a c t t t a c g a a a c a c g g a a a c c g a a g a c c a t t c a t g t t g t t g c a c t g a a g g c g c a a a g g t c t g a a a t g c t t t g t g c c t t t g g c t t c t g g t a a g t a c a a c a a c g

Bpu10I 6180 t c a g g t c g c a g a c g t t t t g c a g c a g c a g t c g c t t c a c g t t c g c t c g c g t a t c g g t g a t t c a g t c c a g c g t c t g c a a a a c g t c g t c g t c a g c g a a g t g c a a g c g a g c g c a t a g c c a c t a a g

6240 a t t c t g c t a a c c a g t a a g g c a a c c c c g c c a g c c t a g c c g g g t c c t c a a c g a c a g g a g c a c t a a g a c g a t t g g t c a t t c c g t t g g g g c g g t c g g a t c g g c c c a g g a g t t g c t g t c c t c g t g

MscI*FspAI 6300 lacIq promoter g a t c a t g c g c a c c c g t g g c c a g g a c c c a a c g c t g c c c g a a a t t c c g a c a c c a t c g a a t g g c t a g t a c g c g t g g g c a c c g g t c c t g g g t t g c g a c g g g c t t t a a g g c t g t g g t a g c t t a c c

6360 lacIq promoter t g c a a a a c c t t t c g c g g t a t g g c a t g a t a g c g c c c g g a a g a g a g t c a a t t c a g g g t g g t g a c g t t t t g g a a a g c g c c a t a c c g t a c t a t c g c g g g c c t t c t c t c a g t t a a g t c c c a c c a c

6420 1 5 10 15 MKPVTLYDVAEYAGVSYQT lacIq promoter lacI T G C T A C A G C G T C T C A T A C G G LacI-R a a t g t g a a a c c a g t a a c g t t a t a c g a t g t c g c a g a g t a t g c c g g t g t c t c t t a t c a g a c c t t a c a c t t t g g t c a t t g c a a t a t g c t a c a g c g t c t c a t a c g g c c a c a g a g a a t a g t c t g g

6480 20 25 30 35 VSRVVNQASHVSAKTREKVE lacI g t t t c c c g c g t g g t g a a c c a g g c c a g c c a c g t t t c t g c g a a a a c g c g g g a a a a a g t g g a a c a a a g g g c g c a c c a c t t g g t c c g g t c g g t g c a a a g a c g c t t t t g c g c c c t t t t t c a c c t t

6540 40 45 50 55 AAMAELNYIPNRVAQQLAGK lacI MAELNYIPNRVAQQLAGK g c g g c g a t g g c g g a g c t g a a t t a c a t t c c c a a c c g c g t g g c a c a a c a a c t g g c g g g c a a a c g c c g c t a c c g c c t c g a c t t a a t g t a a g g g t t g g c g c a c c g t g t t g t t g a c c g c c c g t t t

6600 60 65 70 75 QSLLIGVATSSLALHAPSQI lacI QSLLIGVATSSLALHAPSQI c a g t c g t t g c t g a t t g g c g t t g c c a c c t c c a g t c t g g c c c t g c a c g c g c c g t c g c a a a t t g t c a g c a a c g a c t a a c c g c a a c g g t g g a g g t c a g a c c g g g a c g t g c g c g g c a g c g t t t a a

6660 80 85 90 95 VAAIKSRADQLGASVVVSMV lacI VAAIKSRADQLGASVVVSMV g t c g c g g c g a t t a a a t c t c g c g c c g a t c a a c t g g g t g c c a g c g t g g t g g t g t c g a t g g t a c a g c g c c g c t a a t t t a g a g c g c g g c t a g t t g a c c c a c g g t c g c a c c a c c a c a g c t a c c a t

6720 100 105 110 115 ERSGVEACKAAVHNLLAQRV lacI ERSGVEACKAAVHNLLAQRV g a a c g a a g c g g c g t c g a a g c c t g t a a a g c g g c g g t g c a c a a t c t t c t c g c g c a a c g c g t c c t t g c t t c g c c g c a g c t t c g g a c a t t t c g c c g c c a c g t g t t a g a a g a g c g c g t t g c g c a g

6780 120 125 130 135 SGLIINYPLDDQDAIAVEAA lacI SGLIINYPLDDQDAIAVEAA a g t g g g c t g a t c a t t a a c t a t c c g c t g g a t g a c c a g g a t g c c a t t g c t g t g g a a g c t g c c t c a c c c g a c t a g t a a t t g a t a g g c g a c c t a c t g g t c c t a c g g t a a c g a c a c c t t c g a c g g

6840 140 145 150 155 CTNVPALFLDVSDQTPINSI lacI CTNVPALFLDVSDQTPINSI t g c a c t a a t g t t c c g g c g t t a t t t c t t g a t g t c t c t g a c c a g a c a c c c a t c a a c a g t a t t a c g t g a t t a c a a g g c c g c a a t a a a g a a c t a c a g a g a c t g g t c t g t g g g t a g t t g t c a t a a

BstEII 6900 160 165 170 175 IFSHEDGTRLGVEHLVALGH lacI IFSHEDGTRLGVEHLVALGH a t t t t c t c c c a t g a a g a c g g t a c g c g a c t g g g c g t g g a g c a t c t g g t c g c a t t g g g t c a c t a a a a g a g g g t a c t t c t g c c a t g c g c t g a c c c g c a c c t c g t a g a c c a g c g t a a c c c a g t g

PspOMIApaI 6960 180 185 190 195 QQIALLAGPLSSVSARLRLA lacI QQIALLAGPLSSVSARLRLA c a g c a a a t c g c g c t g t t a g c g g g c c c a t t a a g t t c t g t c t c g g c g c g t c t g c g t c t g g c t g t c g t t t a g c g c g a c a a t c g c c c g g g t a a t t c a a g a c a g a g c c g c g c a g a c g c a g a c c g a

7020 200 205 210 215 GWHKYLTRNQIQPIAEREGD lacI GWHKYLTRNQIQPIAEREGD g g c t g g c a t a a a t a t c t c a c t c g c a a t c a a a t t c a g c c g a t a g c g g a a c g g g a a g g c g a c c c g a c c g t a t t t a t a g a g t g a g c g t t a g t t t a a g t c g g c t a t c g c c t t g c c c t t c c g c t g

7080 220 225 230 235 WSAMSGFQQTMQMLNEGIVP lacI WSAMSGFQQTMQMLNEGIVP t g g a g t g c c a t g t c c g g t t t t c a a c a a a c c a t g c a a a t g c t g a a t g a g g g c a t c g t t c c c a c c t c a c g g t a c a g g c c a a a a g t t g t t t g g t a c g t t t a c g a c t t a c t c c c g t a g c a a g g g

7140 240 245 250 255 TAMLVANDQMALGAMRAITE lacI TAMLVANDQMALGAMRAITE a c t g c g a t g c t g g t t g c c a a c g a t c a g a t g g c g c t g g g c g c a a t g c g c g c c a t t a c c g a g t g a c g c t a c g a c c a a c g g t t g c t a g t c t a c c g c g a c c c g c g t t a c g c g c g g t a a t g g c t c

EcoRV 7200 260 265 270 275 SGLRVGADISVVGYDDTEDS lacI SGLRVGADISVVGYDDTEDS t c c g g g c t g c g c g t t g g t g c g g a t a t c t c g g t a g t g g g a t a c g a c g a t a c c g a a g a c a g c a g g c c c g a c g c g c a a c c a c g c c t a t a g a g c c a t c a c c c t a t g c t g c t a t g g c t t c t g t c g

7260 280 285 290 295 SCYIPPLTTIKQDFRLLGQT lacI SCYIPPLTTIKQDFRLLGQT t c a t g t t a t a t c c c g c c g t t a a c c a c c a t c a a a c a g g a t t t t c g c c t g c t g g g g c a a a c c a g t a c a a t a t a g g g c g g c a a t t g g t g g t a g t t t g t c c t a a a a g c g g a c g a c c c c g t t t g g

7320 300 305 310 315 SVDRLLQLSQGQAVKGNQLL lacI SVDRLLQLSQGQAVKGNQLL a g c g t g g a c c g c t t g c t g c a a c t c t c t c a g g g c c a g g c g g t g a a g g g c a a t c a g c t g t t g t c g c a c c t g g c g a a c g a c g t t g a g a g a g t c c c g g t c c g c c a c t t c c c g t t a g t c g a c a a c

KasINarI*SfoIPluTI 7380 320 325 330 335 PVSLVKRKTTLAPNTQTASP lacI PVSLVKRKTTLAPNTQTASP c c c g t c t c a c t g g t g a a a a g a a a a a c c a c c c t g g c g c c c a a t a c g c a a a c c g c c t c t c c c g g g c a g a g t g a c c a c t t t t c t t t t t g g t g g g a c c g c g g g t t a t g c g t t t g g c g g a g a g g g

7440 340 345 350 355 RALADSLMQLARQVSRLESG lacI RALADSLMQLARQVSRLESG c g c g c g t t g g c c g a t t c a t t a a t g c a g c t g g c a c g a c a g g t t t c c c g a c t g g a a a g c g g g g c g c g c a a c c g g c t a a g t a a t t a c g t c g a c c g t g c t g t c c a a a g g g c t g a c c t t t c g c c c

7500 360 Q* lacI Q* c a g t g a g c g c a a c g c a a t t a a t g t a a g t t a g c t c a c t c a t t a g g c a c a a t t c t c a t g t t t g t c a c t c g c g t t g c g t t a a t t a c a t t c a a t c g a g t g a g t a a t c c g t g t t a a g a g t a c a a a

7560 g a c a g c t t a t c a t c g a c t g c a c g g t g c a c c a a t g c t t c t g g c g t c a g g c a g c c a t c g g a a c t g t c g a a t a g t a g c t g a c g t g c c a c g t g g t t a c g a a g a c c g c a g t c c g t c g g t a g c c t t

7620 g c t g t g g t a t g g c t g t g c a g g t c g t a a a t c a c t g c a t a a t t c g t g t c g c t c a a g g c g c a c c g a c a c c a t a c c g a c a c g t c c a g c a t t t a g t g a c g t a t t a a g c a c a g c g a g t t c c g c g t g

3ʹ 5ʹ 7639 t c c c g t t c t g g a t a a t g t t a g g g c a a g a c c t a t t a c a a

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.