Addgene mcry1K31R HA tag Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: mcry1K31R HA tag Sequencing Result


Map View

pRS-marker (7257 .. 7276) BglII (7225) SgrDI (7211) SspI (7094) Amp-R (6840 .. 6859) ScaI (6770) AhdI (6290) pBR322ori-F (5538 .. 5557) PciI (5397) L4440 (5287 .. 5304) CAP binding site M13/pUC Reverse (5069 .. 5091) lac operator M13 Reverse (5056 .. 5072) BstZ17I (5018) EBV-rev (4977 .. 4996) SV40pA-R (4923 .. 4942) Neo-F (4580 .. 4599) BssHII (4441) PflFI - Tth111I (4162) PluTI (4047) SfoI (4045) NarI (4044) KasI (4043) Neo-R (3970 .. 3989) SmaI (3857) TspMI - XmaI (3855) AvrII (3834) SV40pro-F (3762 .. 3781) MfeI (35) Bpu10I (54) CMV enhancer NdeI (358) SnaBI (464) CMV-F (643 .. 663) T7 (737 .. 756) HindIII (776) Acc65I (782) KpnI (786) BamHI (794) EcoRV (829) NotI (844) PflMI (1027) EcoNI (1615) HA XbaI (2702) PspOMI (2708) ApaI (2712) PmeI (2786) BGH-rev (2802 .. 2819) BbsI (2997) F1ori-R (3165 .. 3184) DraIII (3311) F1ori-F (3375 .. 3396) pBABE 3' (3515 .. 3535) CsiI - SexAI * (3601) mcry1K31R HA tag 7339 bp

Sequence View

Bpu10IMfeI 5ʹ 3ʹ 60 a a a t t t a a g c t a c a a c a a g g c a a g g c t t g a c c g a c a a t t g c a t g a a g a a t c t g c t t a g g g t t t a a a t t c g a t g t t g t t c c g t t c c g a a c t g g c t g t t a a c g t a c t t c t t a g a c g a a t c c c

120 CMV enhancer t t a g g c g t t t t g c g c t g c t t c g c g a t g t a c g g g c c a g a t a t a c g c g t t g a c a t t g a t t a t a a t c c g c a a a a c g c g a c g a a g c g c t a c a t g c c c g g t c t a t a t g c g c a a c t g t a a c t a a t a

180 CMV enhancer t g a c t a g t t a t t a a t a g t a a t c a a t t a c g g g g t c a t t a g t t c a t a g c c c a t a t a t g g a g t a c t g a t c a a t a a t t a t c a t t a g t t a a t g c c c c a g t a a t c a a g t a t c g g g t a t a t a c c t c a

240 CMV enhancer t c c g c g t t a c a t a a c t t a c g g t a a a t g g c c c g c c t g g c t g a c c g c c c a a c g a c c c c c g c c a g g c g c a a t g t a t t g a a t g c c a t t t a c c g g g c g g a c c g a c t g g c g g g t t g c t g g g g g c g g

300 CMV enhancer c a t t g a c g t c a a t a a t g a c g t a t g t t c c c a t a g t a a c g c c a a t a g g g a c t t t c c a t t g a c g t a a c t g c a g t t a t t a c t g c a t a c a a g g g t a t c a t t g c g g t t a t c c c t g a a a g g t a a c t g

NdeI 360 CMV enhancer g t c a a t g g g t g g a g t a t t t a c g g t a a a c t g c c c a c t t g g c a g t a c a t c a a g t g t a t c a t a c a g t t a c c c a c c t c a t a a a t g c c a t t t g a c g g g t g a a c c g t c a t g t a g t t c a c a t a g t a t

420 CMV enhancer t g c c a a g t a c g c c c c c t a t t g a c g t c a a t g a c g g t a a a t g g c c c g c c t g g c a t t a t g c c c a c g g t t c a t g c g g g g g a t a a c t g c a g t t a c t g c c a t t t a c c g g g c g g a c c g t a a t a c g g g

SnaBI 480 CMV enhancer a g t a c a t g a c c t t a t g g g a c t t t c c t a c t t g g c a g t a c a t c t a c g t a t t a g t c a t c g c t a t c a t g t a c t g g a a t a c c c t g a a a g g a t g a a c c g t c a t g t a g a t g c a t a a t c a g t a g c g a t

540 CMV enhancer CMV promoter t t a c c a t g g t g a t g c g g t t t t g g c a g t a c a t c a a t g g g c g t g g a t a g c g g t t t g a c t c a c a a t g g t a c c a c t a c g c c a a a a c c g t c a t g t a g t t a c c c g c a c c t a t c g c c a a a c t g a g t g

600 CMV promoter g g g g a t t t c c a a g t c t c c a c c c c a t t g a c g t c a a t g g g a g t t t g t t t t g g c a c c a a a a t c c c c c t a a a g g t t c a g a g g t g g g g t a a c t g c a g t t a c c c t c a a a c a a a a c c g t g g t t t t a g

660 CMV promoter C G C A A A T G G G C G G T A G G C CMV-F a a c g g g a c t t t c c a a a a t g t c g t a a c a a c t c c g c c c c a t t g a c g c a a a t g g g c g g t a g g c t t g c c c t g a a a g g t t t t a c a g c a t t g t t g a g g c g g g g t a a c t g c g t t t a c c c g c c a t c c g

720 CMV promoter G T G CMV-F g t g t a c g g t g g g a g g t c t a t a t a a g c a g a g c t c t c t g g c t a a c t a g a g a a c c c a c t g c t t c a c a t g c c a c c c t c c a g a t a t a t t c g t c t c g a g a g a c c g a t t g a t c t c t t g g g t g a c g a a

HindIII 780 T7 promoter T A A T A C G A C T C A C T A T A G G G T7 a c t g g c t t a t c g a a a t t a a t a c g a c t c a c t a t a g g g a g a c c c a a g c t g g c t a g t t a a g c t t g a c c g a a t a g c t t t a a t t a t g c t g a g t g a t a t c c c t c t g g g t t c g a c c g a t c a a t t c g a

EcoRVBamHIAcc65IKpnI 840 t g g t a c c g a g c t c g g a t c c a c t a g t c c a g t g t g g t g g a a t t c t g c a g a t a t c c a g c a c a g a c c a t g g c t c g a g c c t a g g t g a t c a g g t c a c a c c a c c t t a a g a c g t c t a t a g g t c g t g t c

NotI 900 MGVNAVHWFRRGLRLH t g g c g g c c g c a c c a t g g g t g t t a a t g c c g t c c a t t g g t t t a g a a g a g g c c t c c g t c t g c a a c c g c c g g c g t g g t a c c c a c a a t t a c g g c a g g t a a c c a a a t c t t c t c c g g a g g c a g a c g t

960 DNPALRECIQGADTIRCVYI t g a c a a t c c g g c t c t g c g c g a a t g t a t c c a g g g t g c t g a c a c g a t c a g g t g c g t t t a c a t a c t g t t a g g c c g a g a c g c g c t t a c a t a g g t c c c a c g a c t g t g c t a g t c c a c g c a a a t g t a

1020 LDPWFAGSSNVGINRWRFLL t c t t g a c c c t t g g t t t g c a g g c a g c a g c a a c g t a g g c a t c a a t a g a t g g c g t t t c c t t c t a g a a c t g g g a a c c a a a c g t c c g t c g t c g t t g c a t c c g t a g t t a t c t a c c g c a a a g g a a g a

PflMI 1080 QCLEDLDANLRRLNSRLFVI c c a g t g t c t g g a g g a t t t g g a t g c c a a t c t g c g t a g g t t g a a c t c c c g t t t g t t t g t t a t g g t c a c a g a c c t c c t a a a c c t a c g g t t a g a c g c a t c c a a c t t g a g g g c a a a c a a a c a a t a

1140 RGQPADVFPRLFREWNITRL t c g c g g c c a g c c t g c t g a c g t g t t t c c c c g c c t g t t c c g c g a a t g g a a c a t t a c a c g t c t a g c g c c g g t c g g a c g a c t g c a c a a a g g g g c g g a c a a g g c g c t t a c c t t g t a a t g t g c a g a

1200 SIEYDSEPFGRERDAAIRRL g a g c a t t g a g t a c g a c t c t g a g c c a t t t g g t c g t g a a c g c g a t g c c g c t a t c c g c a g a c t c t c g t a a c t c a t g c t g a g a c t c g g t a a a c c a g c a c t t g c g c t a c g g c g a t a g g c g t c t g a

1260 ATEAGVEVIVRISHTLYDLD c g c c a c a g a g g c t g g a g t c g a g g t t a t c g t g a g a a t a a g c c a c a c a c t g t a c g a c c t c g a g c g g t g t c t c c g a c c t c a g c t c c a a t a g c a c t c t t a t t c g g t g t g t g a c a t g c t g g a g c t

1320 RIIELNGGQPPLTYRRFQTL c a g g a t c a t a g a g c t c a a c g g t g g t c a a c c t c c g c t g a c t t a c a g g c g c t t t c a g a c c c t g t c c t a g t a t c t c g a g t t g c c a c c a g t t g g a g g c g a c t g a a t g t c c g c g a a a g t c t g g g a

1380 VSRMEPLEMPADTITSDVIG c g t a a g c a g a a t g g a g c c g c t c g a a a t g c c t g c c g a t a c a a t c a c c t c c g a c g t g a t c g g g c a t t c g t c t t a c c t c g g c g a g c t t t a c g g a c g g c t a t g t t a g t g g a g g c t g c a c t a g c c

1440 RCMTPLSDDHDERYGVPSLE c c g c t g c a t g a c g c c a c t t t c g g a c g a c c a c g a t g a g a g a t a c g g a g t a c c t a g c t t g g a g g c g a c g t a c t g c g g t g a a a g c c t g c t g g t g c t a c t c t c t a t g c c t c a t g g a t c g a a c c t

1500 ELGFDTDGLSSAVWPGGETE a g a g c t c g g a t t t g a c a c t g a t g g a c t g t c a a g t g c g g t g t g g c c a g g t g g t g a a a c c g a t c t c g a g c c t a a a c t g t g a c t a c c t g a c a g t t c a c g c c a c a c c g g t c c a c c a c t t t g g c t

1560 ALTRLERHLERRAWVANFER g g c a t t g a c c a g g c t t g a g c g t c a c c t t g a a c g c c g t g c t t g g g t t g c c a a c t t c g a a a g c c g t a a c t g g t c c g a a c t c g c a g t g g a a c t t g c g g c a c g a a c c c a a c g g t t g a a g c t t t c

EcoNI 1620 PRMNANSLLASPTGLSPYLR g c c a c g t a t g a a t g c g a a c t c t c t c c t g g c t t c t c c t a c t g g a c t c t c g c c c t a c c t t a g c g g t g c a t a c t t a c g c t t g a g a g a g g a c c g a a g a g g a t g a c c t g a g a g c g g g a t g g a a t c

1680 FGCLSCRLFYFRLTDLYRRV g t t t g g c t g c c t g a g t t g t c g t t t g t t c t a t t t c c g c c t g a c c g a c c t c t a t a g a c g c g t c a a a c c g a c g g a c t c a a c a g c a a a c a a g a t a a a g g c g g a c t g g c t g g a g a t a t c t g c g c a

1740 RRNSSPPLSLYGQLLWREFF g c g t c g t a a c a g t t c c c c a c c a c t c t c a t t g t a t g g t c a g c t c t t g t g g a g g g a a t t c t t c g c a g c a t t g t c a a g g g g t g g t g a g a g t a a c a t a c c a g t c g a g a a c a c c t c c c t t a a g a a

1800 YTAATNNPRFDRMEGNPICV c t a c a c t g c g g c g a c a a a c a a t c c c a g a t t c g a t c g t a t g g a a g g a a a t c c c a t c t g c g t g a t g t g a c g c c g c t g t t t g t t a g g g t c t a a g c t a g c a t a c c t t c c t t t a g g g t a g a c g c a

1860 QIPWDRNPEALARWAEGRTG t c a g a t c c c t t g g g a c a g a a a c c c c g a g g c g t t g g c a c g t t g g g c t g a a g g a c g c a c c g g a g t c t a g g g a a c c c t g t c t t t g g g g c t c c g c a a c c g t g c a a c c c g a c t t c c t g c g t g g c c

1920 FPWIDAIMTQLRQEGWIHHL t t t c c c c t g g a t a g a c g c c a t c a t g a c c c a g c t g c g c c a a g a g g g t t g g a t t c a c c a t c t a a a g g g g a c c t a t c t g c g g t a g t a c t g g g t c g a c g c g g t t c t c c c a a c c t a a g t g g t a g a

1980 ARHAVACFLTRGDLWISWEE g g c t a g g c a c g c a g t c g c c t g t t t c c t c a c c a g a g g c g a c c t t t g g a t t t c a t g g g a g g a c c g a t c c g t g c g t c a g c g g a c a a a g g a g t g g t c t c c g c t g g a a a c c t a a a g t a c c c t c c t

2040 GMRVFEELLLDADWSINAGS g g g c a t g c g t g t a t t c g a a g a g t t g c t c c t t g a t g c t g a t t g g t c c a t t a a c g c a g g c t c c c c g t a c g c a c a t a a g c t t c t c a a c g a g g a a c t a c g a c t a a c c a g g t a a t t g c g t c c g a g

2100 WMWLSCSSFFQQFFHCYCPV t t g g a t g t g g t t g t c t t g c t c c t c g t t c t t c c a a c a a t t c t t c c a c t g c t a t t g c c c a g t a a c c t a c a c c a a c a g a a c g a g g a g c a a g a a g g t t g t t a a g a a g g t g a c g a t a a c g g g t c a

2160 GFGRRTDPNGDYIRRYLPVL g g g t t t c g g a a g g a g g a c c g a c c c g a a c g g t g a c t a c a t c c g c c g c t a t c t g c c g g t c c t c c c a a a g c c t t c c t c c t g g c t g g g c t t g c c a c t g a t g t a g g c g g c g a t a g a c g g c c a g g a

2220 RGFPARYIYDPWNAPEGIQR t a g g g g c t t c c c t g c g c g t t a c a t c t a c g a c c c c t g g a a t g c t c c c g a a g g a a t t c a g c g a t c c c c g a a g g g a c g c g c a a t g t a g a t g c t g g g g a c c t t a c g a g g g c t t c c t t a a g t c g c

2280 VARCLIGVNYPRPMVNHAEA t g t c g c t c g c t g c c t g a t t g g t g t c a a c t a c c c t c g t c c t a t g g t c a a t c a t g c c g a g g c a c a g c g a g c g a c g g a c t a a c c a c a g t t g a t g g g a g c a g g a t a c c a g t t a g t a c g g c t c c g

2340 SRLNIERMRQIYQQLSRYRG c t c c c g c t t g a a c a t a g a g a g a a t g c g t c a a a t c t a c c a g c a a c t c t c t c g t t a t a g a g g g a g g g c g a a c t t g t a t c t c t c t t a c g c a g t t t a g a t g g t c g t t g a g a g a g c a a t a t c t c c

2400 LGLLASVPSNSNGNGGLMGY a t t g g g a c t g c t g g c a t c g g t g c c a a g t a a c t c g a a c g g t a a c g g c g g a c t g a t g g g a t a t a a c c c t g a c g a c c g t a g c c a c g g t t c a t t g a g c t t g c c a t t g c c g c c t g a c t a c c c t a t

2460 APGENVPSCSSSGNGGLMGY t g c t c c c g g c g a g a a c g t g c c c t c g t g t t c c t c a a g t g g t a a t g g a g g c c t t a t g g g t t a a c g a g g g c c g c t c t t g c a c g g g a g c a c a a g g a g t t c a c c a t t a c c t c c g g a a t a c c c a a t

2520 APGENVPSCSGGNCSQGSGI c g c t c c c g g t g a a a a c g t g c c a t c t t g c t c t g g t g g a a a c t g c t c c c a a g g c t c c g g c a t g c g a g g g c c a c t t t t g c a c g g t a g a a c g a g a c c a c c t t t g a c g a g g g t t c c g a g g c c g t a

2580 LHYAHGDSQQTHSLRQGRSS t t t g c a c t a c g c c c a c g g t g a c t c t c a g c a g a c t c a t t c t c t g a g g c a g g g t c g t t c a t c a a a c g t g a t g c g g g t g c c a c t g a g a g t c g t c t g a g t a a g a g a c t c c g t c c c a g c a a g t a g

2640 AGTGLSSGRRPSQEEDAQSV a g c t g g c a c g g g c t t g t c c a g c g g c a g g a g g c c c t c c c a a g a g g a a g a t g c a c a g t c a g t t c g a c c g t g c c c g a a c a g g t c g c c g t c c t c c g g g a g g g t t c t c c t t c t a c g t g t c a g t c a

2700 1 5 YPYDVPDYA * HA GPRVQRQSSNYPYDVPDYA* c g g t c c g a g a g t a c a a a g a c a a a g c a g t a a c t a t c c c t a t g a c g t c c c g g a c t a t g c a t a g c c a g g c t c t c a t g t t t c t g t t t c g t c a t t g a t a g g g a t a c t g c a g g g c c t g a t a c g t a t

XbaIPspOMIApaI 2760 1 5 10 EQKLISEEDL (in frame with Myc) NMHTG Myc a t c t a g a g g g c c c t t c g a a c a a a a a c t c a t c t c a g a a g a g g a t c t g a a t a t g c a t a c c g g t a g a t c t c c c g g g a a g c t t g t t t t t g a g t a g a g t c t t c t c c t a g a c t t a t a c g t a t g g c c

PmeI 2820 1 5 HHHHHH * 6xHis bGH poly(A) signal G G A G C T G A C A C G G A A G A T BGH-rev t c a t c a t c a c c a t c a c c a t t g a g t t t a a a c c c g c t g a t c a g c c t c g a c t g t g c c t t c t a g a g t a g t a g t g g t a g t g g t a a c t c a a a t t t g g g c g a c t a g t c g g a g c t g a c a c g g a a g a t c

2880 bGH poly(A) signal t t g c c a g c c a t c t g t t g t t t g c c c c t c c c c c g t g c c t t c c t t g a c c c t g g a a g g t g c c a c a a c g g t c g g t a g a c a a c a a a c g g g g a g g g g g c a c g g a a g g a a c t g g g a c c t t c c a c g g t g

2940 bGH poly(A) signal t c c c a c t g t c c t t t c c t a a t a a a a t g a g g a a a t t g c a t c g c a t t g t c t g a g t a g g t g t c a a g g g t g a c a g g a a a g g a t t a t t t t a c t c c t t t a a c g t a g c g t a a c a g a c t c a t c c a c a g t

BbsI 3000 bGH poly(A) signal t t c t a t t c t g g g g g g t g g g g t g g g g c a g g a c a g c a a g g g g g a g g a t t g g g a a g a c a a t a g a a g a t a a g a c c c c c c a c c c c a c c c c g t c c t g t c g t t c c c c c t c c t a a c c c t t c t g t t a t c

3060 bGH poly(A) signal c a g g c a t g c t g g g g a t g c g g t g g g c t c t a t g g c t t c t g a g g c g g a a a g a a c c a g c t g g g g g t c c g t a c g a c c c c t a c g c c a c c c g a g a t a c c g a a g a c t c c g c c t t t c t t g g t c g a c c c c

3120 f1 ori c t c t a g g g g g t a t c c c c a c g c g c c c t g t a g c g g c g c a t t a a g c g c g g c g g g t g t g g t g g t g a g a t c c c c c a t a g g g g t g c g c g g g a c a t c g c c g c g t a a t t c g c g c c g c c c a c a c c a c c a

3180 f1 ori G A G G A A A G C G A A A G A A F1ori-R t a c g c g c a g c g t g a c c g c t a c a c t t g c c a g c g c c c t a g c g c c c g c t c c t t t c g c t t t c t t a t g c g c g t c g c a c t g g c g a t g t g a a c g g t c g c g g g a t c g c g g g c g a g g a a a g c g a a a g a a

3240 f1 ori G G G A F1ori-R c c c t t c c t t t c t c g c c a c g t t c g c c g g c t t t c c c c g t c a a g c t c t a a a t c g g g g g c t c c c g g g a a g g a a a g a g c g g t g c a a g c g g c c g a a a g g g g c a g t t c g a g a t t t a g c c c c c g a g g g

3300 f1 ori t t t a g g g t t c c g a t t t a g t g c t t t a c g g c a c c t c g a c c c c a a a a a a c t t g a t t a g g g t g a a a a t c c c a a g g c t a a a t c a c g a a a t g c c g t g g a g c t g g g g t t t t t t g a a c t a a t c c c a c t

DraIII 3360 f1 ori t g g t t c a c g t a g t g g g c c a t c g c c c t g a t a g a c g g t t t t t c g c c c t t t g a c g t t g g a g t c a c c a a g t g c a t c a c c c g g t a g c g g g a c t a t c t g c c a a a a a g c g g g a a a c t g c a a c c t c a g

3420 f1 ori G T G G A C T C T T G T T C C A A A C T G G F1ori-F c a c g t t c t t t a a t a g t g g a c t c t t g t t c c a a a c t g g a a c a a c a c t c a a c c c t a t c t c g g t g t g c a a g a a a t t a t c a c c t g a g a a c a a g g t t t g a c c t t g t t g t g a g t t g g g a t a g a g c c a

3480 f1 ori c t a t t c t t t t g a t t t a t a a g g g a t t t t g c c g a t t t c g g c c t a t t g g t t a a a a a a t g a g c t g a t a a g a a a a c t a a a t a t t c c c t a a a a c g g c t a a a g c c g g a t a a c c a a t t t t t t a c t c g a

3540 f1 ori SV40 promoter C C T T A C A C A C A G T C A A T C C C A pBABE 3' g a t t t a a c a a a a a t t t a a c g c g a a t t a a t t c t g t g g a a t g t g t g t c a g t t a g g g t g t g g a c t a a a t t g t t t t t a a a t t g c g c t t a a t t a a g a c a c c t t a c a c a c a g t c a a t c c c a c a c c t

3600 SV40 promoter a a g t c c c c a g g c t c c c c a g c a g g c a g a a g t a t g c a a a g c a t g c a t c t c a a t t a g t c a g c a t t c a g g g g t c c g a g g g g t c g t c c g t c t t c a t a c g t t t c g t a c g t a g a g t t a a t c a g t c g t

SexAI*CsiI 3660 SV40 promoter a c c a g g t g t g g a a a g t c c c c a g g c t c c c c a g c a g g c a g a a g t a t g c a a a g c a t g c a t c t c t g g t c c a c a c c t t t c a g g g g t c c g a g g g g t c g t c c g t c t t c a t a c g t t t c g t a c g t a g a g

3720 SV40 promoter SV40 ori a a t t a g t c a g c a a c c a t a g t c c c g c c c c t a a c t c c g c c c a t c c c g c c c c t a a c t c c g c c c t t a a t c a g t c g t t g g t a t c a g g g c g g g g a t t g a g g c g g g t a g g g c g g g g a t t g a g g c g g g

3780 SV40 promoter SV40 ori T A T T T A T G C A G A G G C C G A G SV40pro-F a g t t c c g c c c a t t c t c c g c c c c a t g g c t g a c t a a t t t t t t t t a t t t a t g c a g a g g c c g a g t c a a g g c g g g t a a g a g g c g g g g t a c c g a c t g a t t a a a a a a a a t a a a t a c g t c t c c g g c t c

AvrII 3840 SV40 promoter SV40 ori G SV40pro-F g c c g c c t c t g c c t c t g a g c t a t t c c a g a a g t a g t g a g g a g g c t t t t t t g g a g g c c t a g g c c g g c g g a g a c g g a g a c t c g a t a a g g t c t t c a t c a c t c c t c c g a a a a a a c c t c c g g a t c c g

TspMIXmaISmaI 3900 SV40 promoter t t t t g c a a a a a g c t c c c g g g a g c t t g t a t a t c c a t t t t c g g a t c t g a t c a a g a g a c a g g a a a a a c g t t t t t c g a g g g c c c t c g a a c a t a t a g g t a a a a g c c t a g a c t a g t t c t c t g t c c t

3960 1 5 10 15 MIEQDGLHAGSPAAW NeoR/KanR MIEQDGLHAGSPAAW t g a g g a t c g t t t c g c a t g a t t g a a c a a g a t g g a t t g c a c g c a g g t t c t c c g g c c g c t t g g a c t c c t a g c a a a g c g t a c t a a c t t g t t c t a c c t a a c g t g c g t c c a a g a g g c c g g c g a a c c

4020 20 25 30 35 VERLFGYDWAQQTIGCSDAA NeoR/KanR G A T A A G C C G A T A C T G A C C C G Neo-R VERLFGYDWAQQTIGCSDAA g t g g a g a g g c t a t t c g g c t a t g a c t g g g c a c a a c a g a c a a t c g g c t g c t c t g a t g c c g c c c a c c t c t c c g a t a a g c c g a t a c t g a c c c g t g t t g t c t g t t a g c c g a c g a g a c t a c g g c g g

KasINarISfoIPluTI 4080 40 45 50 55 VFRLSAQGRPVLFVKTDLSG NeoR/KanR VFRLSAQGRPVLFVKTDLSG g t g t t c c g g c t g t c a g c g c a g g g g c g c c c g g t t c t t t t t g t c a a g a c c g a c c t g t c c g g t c a c a a g g c c g a c a g t c g c g t c c c c g c g g g c c a a g a a a a a c a g t t c t g g c t g g a c a g g c c a

4140 60 65 70 75 ALNELQDEAARLSWLATTGV NeoR/KanR ALNELQDEAARLSWLATTGV g c c c t g a a t g a a c t g c a g g a c g a g g c a g c g c g g c t a t c g t g g c t g g c c a c g a c g g g c g t t c g g g a c t t a c t t g a c g t c c t g c t c c g t c g c g c c g a t a g c a c c g a c c g g t g c t g c c c g c a a

Tth111IPflFI 4200 80 85 90 95 PCAAVLDVVTEAGRDWLLLG NeoR/KanR PCAAVLDVVTEAGRDWLLLG c c t t g c g c a g c t g t g c t c g a c g t t g t c a c t g a a g c g g g a a g g g a c t g g c t g c t a t t g g g c g g a a c g c g t c g a c a c g a g c t g c a a c a g t g a c t t c g c c c t t c c c t g a c c g a c g a t a a c c c g

4260 100 105 110 115 EVPGQDLLSSHLAPAEKVSI NeoR/KanR EVPGQDLLSSHLAPAEKVSI g a a g t g c c g g g g c a g g a t c t c c t g t c a t c t c a c c t t g c t c c t g c c g a g a a a g t a t c c a t c c t t c a c g g c c c c g t c c t a g a g g a c a g t a g a g t g g a a c g a g g a c g g c t c t t t c a t a g g t a g

4320 120 125 130 135 MADAMRRLHTLDPATCPFDH NeoR/KanR MADAMRRLHTLDPATCPFDH a t g g c t g a t g c a a t g c g g c g g c t g c a t a c g c t t g a t c c g g c t a c c t g c c c a t t c g a c c a c t a c c g a c t a c g t t a c g c c g c c g a c g t a t g c g a a c t a g g c c g a t g g a c g g g t a a g c t g g t g

4380 140 145 150 155 QAKHRIERARTRMEAGLVDQ NeoR/KanR QAKHRIERARTRMEAGLVDQ c a a g c g a a a c a t c g c a t c g a g c g a g c a c g t a c t c g g a t g g a a g c c g g t c t t g t c g a t c a g g t t c g c t t t g t a g c g t a g c t c g c t c g t g c a t g a g c c t a c c t t c g g c c a g a a c a g c t a g t c

4440 160 165 170 175 DDLDEEHQGLAPAELFARLK NeoR/KanR DDLDEEHQGLAPAELFARLK g a t g a t c t g g a c g a a g a g c a t c a g g g g c t c g c g c c a g c c g a a c t g t t c g c c a g g c t c a a g c t a c t a g a c c t g c t t c t c g t a g t c c c c g a g c g c g g t c g g c t t g a c a a g c g g t c c g a g t t c

BssHII 4500 180 185 190 195 ARMPDGEDLVVTHGDACLPN NeoR/KanR ARMPDGEDLVVTHGDACLPN g c g c g c a t g c c c g a c g g c g a g g a t c t c g t c g t g a c c c a t g g c g a t g c c t g c t t g c c g a a t c g c g c g t a c g g g c t g c c g c t c c t a g a g c a g c a c t g g g t a c c g c t a c g g a c g a a c g g c t t a

4560 200 205 210 215 IMVENGRFSGFIDCGRLGVA NeoR/KanR IMVENGRFSGFIDCGRLGVA a t c a t g g t g g a a a a t g g c c g c t t t t c t g g a t t c a t c g a c t g t g g c c g g c t g g g t g t g g c g t a g t a c c a c c t t t t a c c g g c g a a a a g a c c t a a g t a g c t g a c a c c g g c c g a c c c a c a c c g c

4620 220 225 230 235 DRYQDIALATRDIAEELGGE NeoR/KanR C G T T G G C T A C C C G T G A T A T T Neo-F DRYQDIALATRDIAEELGGE g a c c g c t a t c a g g a c a t a g c g t t g g c t a c c c g t g a t a t t g c t g a a g a g c t t g g c g g c g a a c t g g c g a t a g t c c t g t a t c g c a a c c g a t g g g c a c t a t a a c g a c t t c t c g a a c c g c c g c t t

4680 240 245 250 255 WADRFLVLYGIAAPDSQRIA NeoR/KanR WADRFLVLYGIAAPDSQRIA t g g g c t g a c c g c t t c c t c g t g c t t t a c g g t a t c g c c g c t c c c g a t t c g c a g c g c a t c g c c a c c c g a c t g g c g a a g g a g c a c g a a a t g c c a t a g c g g c g a g g g c t a a g c g t c g c g t a g c g g

4740 260 265 FYRLLDEFF* NeoR/KanR FYRLLDEFF* t t c t a t c g c c t t c t t g a c g a g t t c t t c t g a g c g g g a c t c t g g g g t t c g c g a a a t g a c c g a a a g a t a g c g g a a g a a c t g c t c a a g a a g a c t c g c c c t g a g a c c c c a a g c g c t t t a c t g g c t

4800 c c a a g c g a c g c c c a a c c t g c c a t c a c g a g a t t t c g a t t c c a c c g c c g c c t t c t a t g a a a g g g t t c g c t g c g g g t t g g a c g g t a g t g c t c t a a a g c t a a g g t g g c g g c g g a a g a t a c t t t c

4860 g t t g g g c t t c g g a a t c g t t t t c c g g g a c g c c g g c t g g a t g a t c c t c c a g c g c g g g g a t c t c a a c c c g a a g c c t t a g c a a a a g g c c c t g c g g c c g a c c t a c t a g g a g g t c g c g c c c c t a g a

4920 SV40 poly(A) signal c a t g c t g g a g t t c t t c g c c c a c c c c a a c t t g t t t a t t g c a g c t t a t a a t g g t t a c a a a t a g t a c g a c c t c a a g a a g c g g g t g g g g t t g a a c a a a t a a c g t c g a a t a t t a c c a a t g t t t a t

4980 SV40 poly(A) signal G T G G EBV-rev C G T T A T C G T A G T G T T T A A A G SV40pA-R a a g c a a t a g c a t c a c a a a t t t c a c a a a t a a a g c a t t t t t t t c a c t g c a t t c t a g t t g t g g t t c g t t a t c g t a g t g t t t a a a g t g t t t a t t t c g t a a a a a a a g t g a c g t a a g a t c a a c a c c

BstZ17I 5040 SV40 poly(A) signal T T T G T C C A A A C T C A T C EBV-rev t t t g t c c a a a c t c a t c a a t g t a t c t t a t c a t g t c t g t a t a c c g t c g a c c t c t a g c t a g a g a a a c a g g t t t g a g t a g t t a c a t a g a a t a g t a c a g a c a t a t g g c a g c t g g a g a t c g a t c t c

5100 M13 rev lac operator C A G T A T C G A C A A A G G A C M13 Reverse G G A C A C A C T T T A A C A A T A G G C G A M13/pUC Reverse c t t g g c g t a a t c a t g g t c a t a g c t g t t t c c t g t g t g a a a t t g t t a t c c g c t c a c a a t t c c g a a c c g c a t t a g t a c c a g t a t c g a c a a a g g a c a c a c t t t a a c a a t a g g c g a g t g t t a a g g

5160 -10 -35 lac promoter CAP binding site a c a c a a c a t a c g a g c c g g a a g c a t a a a g t g t a a a g c c t g g g g t g c c t a a t g a g t g a g c t a t g t g t t g t a t g c t c g g c c t t c g t a t t t c a c a t t t c g g a c c c c a c g g a t t a c t c a c t c g a t

5220 CAP binding site a c t c a c a t t a a t t g c g t t g c g c t c a c t g c c c g c t t t c c a g t c g g g a a a c c t g t c g t g c c a t g a g t g t a a t t a a c g c a a c g c g a g t g a c g g g c g a a a g g t c a g c c c t t t g g a c a g c a c g g t

5280 g c t g c a t t a a t g a a t c g g c c a a c g c g c g g g g a g a g g c g g t t t g c g t a t t g g g c g c t c t t c c g a c g t a a t t a c t t a g c c g g t t g c g c g c c c c t c t c c g c c a a a c g c a t a a c c c g c g a g a a g

5340 G A G C G A G T G A C T G A G C G A L4440 c g c t t c c t c g c t c a c t g a c t c g c t g c g c t c g g t c g t t c g g c t g c g g c g a g c g g t a t c a g c g c g a a g g a g c g a g t g a c t g a g c g a c g c g a g c c a g c a a g c c g a c g c c g c t c g c c a t a g t c g

PciI 5400 t c a c t c a a a g g c g g t a a t a c g g t t a t c c a c a g a a t c a g g g g a t a a c g c a g g a a a g a a c a t a g t g a g t t t c c g c c a t t a t g c c a a t a g g t g t c t t a g t c c c c t a t t g c g t c c t t t c t t g t a

5460 ori g t g a g c a a a a g g c c a g c a a a a g g c c a g g a a c c g t a a a a a g g c c g c g t t g c t g g c g t t t t t c a c t c g t t t t c c g g t c g t t t t c c g g t c c t t g g c a t t t t t c c g g c g c a a c g a c c g c a a a a a

5520 ori c c a t a g g c t c c g c c c c c c t g a c g a g c a t c a c a a a a a t c g a c g c t c a a g t c a g a g g t g g c g g g t a t c c g a g g c g g g g g g a c t g c t c g t a g t g t t t t t a g c t g c g a g t t c a g t c t c c a c c g c

5580 ori T T T C T A T G G T C C G C A A A G G G pBR322ori-F a a a c c c g a c a g g a c t a t a a a g a t a c c a g g c g t t t c c c c c t g g a a g c t c c c t c g t g c g c t c t t t g g g c t g t c c t g a t a t t t c t a t g g t c c g c a a a g g g g g a c c t t c g a g g g a g c a c g c g a g

5640 ori t c c t g t t c c g a c c c t g c c g c t t a c c g g a t a c c t g t c c g c c t t t c t c c c t t c g g g a a g c g t a g g a c a a g g c t g g g a c g g c g a a t g g c c t a t g g a c a g g c g g a a a g a g g g a a g c c c t t c g c a

5700 ori g g c g c t t t c t c a t a g c t c a c g c t g t a g g t a t c t c a g t t c g g t g t a g g t c g t t c g c t c c a a c c g c g a a a g a g t a t c g a g t g c g a c a t c c a t a g a g t c a a g c c a c a t c c a g c a a g c g a g g t t

5760 ori g c t g g g c t g t g t g c a c g a a c c c c c c g t t c a g c c c g a c c g c t g c g c c t t a t c c g g t a a c t a c g a c c c g a c a c a c g t g c t t g g g g g g c a a g t c g g g c t g g c g a c g c g g a a t a g g c c a t t g a t

5820 ori t c g t c t t g a g t c c a a c c c g g t a a g a c a c g a c t t a t c g c c a c t g g c a g c a g c c a c t g g t a a a g c a g a a c t c a g g t t g g g c c a t t c t g t g c t g a a t a g c g g t g a c c g t c g t c g g t g a c c a t t

5880 ori c a g g a t t a g c a g a g c g a g g t a t g t a g g c g g t g c t a c a g a g t t c t t g a a g t g g t g g c c t a a g t c c t a a t c g t c t c g c t c c a t a c a t c c g c c a c g a t g t c t c a a g a a c t t c a c c a c c g g a t t

5940 ori c t a c g g c t a c a c t a g a a g a a c a g t a t t t g g t a t c t g c g c t c t g c t g a a g c c a g t t a c c t t g a t g c c g a t g t g a t c t t c t t g t c a t a a a c c a t a g a c g c g a g a c g a c t t c g g t c a a t g g a a

6000 ori c g g a a a a a g a g t t g g t a g c t c t t g a t c c g g c a a a c a a a c c a c c g c t g g t a g c g g t g g t t t g c c t t t t t c t c a a c c a t c g a g a a c t a g g c c g t t t g t t t g g t g g c g a c c a t c g c c a c c a a a

6060 ori t t t t g t t t g c a a g c a g c a g a t t a c g c g c a g a a a a a a a g g a t c t c a a g a a g a t c c t t t g a t a a a a c a a a c g t t c g t c g t c t a a t g c g c g t c t t t t t t t c c t a g a g t t c t t c t a g g a a a c t a

6120 c t t t t c t a c g g g g t c t g a c g c t c a g t g g a a c g a a a a c t c a c g t t a a g g g a t t t t g g t c a t g a a a a g a t g c c c c a g a c t g c g a g t c a c c t t g c t t t t g a g t g c a a t t c c c t a a a a c c a g t a

6180 g a g a t t a t c a a a a a g g a t c t t c a c c t a g a t c c t t t t a a a t t a a a a a t g a a g t t t t a a a t c c t c t a a t a g t t t t t c c t a g a a g t g g a t c t a g g a a a a t t t a a t t t t t a c t t c a a a a t t t a g

6240 285 280 *WHKILSA AmpR *WHKILSA a a t c t a a a g t a t a t a t g a g t a a a c t t g g t c t g a c a g t t a c c a a t g c t t a a t c a g t g a g g c t t a g a t t t c a t a t a t a c t c a t t t g a a c c a g a c t g t c a a t g g t t a c g a a t t a g t c a c t c c g

AhdI 6300 275 270 265 260 GIEAIQRNREDMTAQSGTTY AmpR GIEAIQRNREDMTAQSGTTY a c c t a t c t c a g c g a t c t g t c t a t t t c g t t c a t c c a t a g t t g c c t g a c t c c c c g t c g t g t a t g g a t a g a g t c g c t a g a c a g a t a a a g c a a g t a g g t a t c a a c g g a c t g a g g g g c a g c a c a t

6360 255 250 245 240 IVVIRSPKGDPGLAAIIGRS AmpR IVVIRSPKGDPGLAAIIGRS g a t a a c t a c g a t a c g g g a g g g c t t a c c a t c t g g c c c c a g t g c t g c a a t g a t a c c g c g a g a c t a t t g a t g c t a t g c c c t c c c g a a t g g t a g a c c g g g g t c a c g a c g t t a c t a t g g c g c t c t

6420 235 230 225 220 GREGAGSKDAIFWGAPLASR AmpR GREGAGSKDAIFWGAPLASR c c c a c g c t c a c c g g c t c c a g a t t t a t c a g c a a t a a a c c a g c c a g c c g g a a g g g c c g a g c g g g g t g c g a g t g g c c g a g g t c t a a a t a g t c g t t a t t t g g t c g g t c g g c c t t c c c g g c t c g c

6480 215 210 205 200 LLPGAVKDAEMWDILQQRSA AmpR LLPGAVKDAEMWDILQQRSA c a g a a g t g g t c c t g c a a c t t t a t c c g c c t c c a t c c a g t c t a t t a a t t g t t g c c g g g a a g c g t c t t c a c c a g g a c g t t g a a a t a g g c g g a g g t a g g t c a g a t a a t t a a c a a c g g c c c t t c g

6540 195 190 185 180 LTLLEGTLLKRLTTAMAVPM AmpR LTLLEGTLLKRLTTAMAVPM t a g a g t a a g t a g t t c g c c a g t t a a t a g t t t g c g c a a c g t t g t t g c c a t t g c t a c a g g c a t a t c t c a t t c a t c a a g c g g t c a a t t a t c a a a c g c g t t g c a a c a a c g g t a a c g a t g t c c g t a

6600 175 170 165 160 TTDREDNPIAENLEPEWRDL AmpR TTDREDNPIAENLEPEWRDL c g t g g t g t c a c g c t c g t c g t t t g g t a t g g c t t c a t t c a g c t c c g g t t c c c a a c g a t c a a g g c a c c a c a g t g c g a g c a g c a a a c c a t a c c g a a g t a a g t c g a g g c c a a g g g t t g c t a g t t c

6660 155 150 145 140 RTVHDGMNHLFATLEKPGGI AmpR RTVHDGMNHLFATLEKPGGI g c g a g t t a c a t g a t c c c c c a t g t t g t g c a a a a a a g c g g t t a g c t c c t t c g g t c c t c c g a t c g c t c a a t g t a c t a g g g g g t a c a a c a c g t t t t t t c g c c a a t c g a g g a a g c c a g g a g g c t a

6720 135 130 125 120 TTLLLNAATNDSMTIAASCL AmpR TTLLLNAATNDSMTIAASCL c g t t g t c a g a a g t a a g t t g g c c g c a g t g t t a t c a c t c a t g g t t a t g g c a g c a c t g c a t a a g c a a c a g t c t t c a t t c a a c c g g c g t c a c a a t a g t g a g t a c c a a t a c c g t c g t g a c g t a t t

ScaI 6780 115 110 105 100 ERVTMGDTLHKETVPSYEVL AmpR ERVTMGDTLHKETVPSYEVL t t c t c t t a c t g t c a t g c c a t c c g t a a g a t g c t t t t c t g t g a c t g g t g a g t a c t c a a c c a a a a g a g a a t g a c a g t a c g g t a g g c a t t c t a c g a a a a g a c a c t g a c c a c t c a t g a g t t g g t t

6840 95 90 85 80 DNQSYHIRRGLQEQGADIRS AmpR A Amp-R DNQSYHIRRGLQEQGADIRS g t c a t t c t g a g a a t a g t g t a t g c g g c g a c c g a g t t g c t c t t g c c c g g c g t c a a t a c g g g a c a g t a a g a c t c t t a t c a c a t a c g c c g c t g g c t c a a c g a g a a c g g g c c g c a g t t a t g c c c t

6900 75 70 65 60 LVAGCLLVKFTSMMPFREEP AmpR T A A T A C C G C G C C A C A T A G C Amp-R LVAGCLLVKFTSMMPFREEP t a a t a c c g c g c c a c a t a g c a g a a c t t t a a a a g t g c t c a t c a t t g g a a a a c g t t c t t c g g g a t t a t g g c g c g g t g t a t c g t c t t g a a a t t t t c a c g a g t a g t a a c c t t t t g c a a g a a g c c c

6960 55 50 45 40 RFSELIKGSNLDLEIYGVRA AmpR RFSELIKGSNLDLEIYGVRA g c g a a a a c t c t c a a g g a t c t t a c c g c t g t t g a g a t c c a g t t c g a t g t a a c c c a c t c g t g c c g c t t t t g a g a g t t c c t a g a a t g g c g a c a a c t c t a g g t c a a g c t a c a t t g g g t g a g c a c g

7020 35 30 25 GLQDEADKVKVLTEPH 20 signal sequence AFVP AmpR GLQDEADKVKVLTEPHAFVP a c c c a a c t g a t c t t c a g c a t c t t t t a c t t t c a c c a g c g t t t c t g g g t g a g c a a a a a c a g g t g g g t t g a c t a g a a g t c g t a g a a a a t g a a a g t g g t c g c a a a g a c c c a c t c g t t t t t g t c c

7080 15 10 5 1 signal sequence LCFAAFFPILAVRFHQISM AmpR AmpR promoter LCFAAFFPILAVRFHQISM a a g g c a a a a t g c c g c a a a a a a g g g a a t a a g g g c g a c a c g g a a a t g t t g a a t a c t c a t a c t t t c c g t t t t a c g g c g t t t t t t c c c t t a t t c c c g c t g t g c c t t t a c a a c t t a t g a g t a t g a

SspI 7140 AmpR promoter c t t c c t t t t t c a a t a t t a t t g a a g c a t t t a t c a g g g t t a t t g t c t c a t g a g c g g a t a c a t g a a g g a a a a a g t t a t a a t a a c t t c g t a a a t a g t c c c a a t a a c a g a g t a c t c g c c t a t g t a

7200 AmpR promoter a t t t g a a t g t a t t t a g a a a a a t a a a c a a a t a g g g g t t c c g c g c a c a t t t c c c c g a a a a g t t a a a c t t a c a t a a a t c t t t t t a t t t g t t t a t c c c c a a g g c g c g t g t a a a g g g g c t t t t c a

BglIISgrDI 7260 A T G T pRS-marker g c c a c c t g a c g t c g a c g g a t c g g g a g a t c t c c c g a t c c c c t a t g g t g c a c t c t c a g t a c a c g g t g g a c t g c a g c t g c c t a g c c c t c t a g a g g g c t a g g g g a t a c c a c g t g a g a g t c a t g t

7320 T A G A C G A G A C T A C G G C pRS-marker a t c t g c t c t g a t g c c g c a t a g t t a a g c c a g t a t c t g c t c c c t g c t t g t g t g t t g g a g g t c t a g a c g a g a c t a c g g c g t a t c a a t t c g g t c a t a g a c g a g g g a c g a a c a c a c a a c c t c c a g

3ʹ 5ʹ 7339 g c t g a g t a g t g c g c g a g c a c g a c t c a t c a c g c g c t c g t

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.