Addgene [pSK467] pLJC5 HA-Depdc5 Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: [pSK467] pLJC5 HA-Depdc5 Sequencing Result


Map View

pBR322ori-F (12,785 .. 12,804) Amp-R (11,483 .. 11,502) AmpR promoter F1ori-F (10,976 .. 10,997) F1ori-R (10,766 .. 10,785) M13/pUC Forward (10,530 .. 10,552) M13 Forward (10,521 .. 10,538) M13 fwd T7 (10,492 .. 10,511) T7 promoter SfiI (10,424) SV40 ori EBV-rev (10,267 .. 10,286) SV40 poly(A) signal SV40pA-R (10,213 .. 10,232) KpnI (9804) Acc65I (9800) Puro-F (9640 .. 9660) RsrII (9260) BsiWI (9200) Puro-R (9144 .. 9163) hPGK-F (9041 .. 9059) AscI (8957) EcoRI (8405) BsrGI (8038) PmlI (7243) NdeI (6974) L4440 (154 .. 171) CAP binding site lac promoter M13/pUC Reverse (367 .. 389) lac operator M13 Reverse (386 .. 402) M13 rev T3 (421 .. 441) T3 promoter 5' LTR (truncated) HIV-1 Ψ NruI * (1075) MfeI (1427) AleI (1815) BspDI * - ClaI * (2261) UbC promoter hUBCpro-F (3340 .. 3359) NheI (3509) BmtI (3513) AgeI (3518) Kozak sequence SalI (3586) [pSK467] pLJC5 HA-Depdc5 12,884 bp

Sequence View

5ʹ 3ʹ 60 a a c g c c a g c a a c g c g g c c t t t t t a c g g t t c c t g g c c t t t t g c t g g c c t t t t g c t c a c a t g t t g c g g t c g t t g c g c c g g a a a a a t g c c a a g g a c c g g a a a a c g a c c g g a a a a c g a g t g t a c

120 t t c t t t c c t g c g t t a t c c c c t g a t t c t g t g g a t a a c c g t a t t a c c g c c t t t g a g t g a g c t a a g a a a g g a c g c a a t a g g g g a c t a a g a c a c c t a t t g g c a t a a t g g c g g a a a c t c a c t c g a

180 A G C G A G T C A G T G A G C G A G L4440 g a t a c c g c t c g c c g c a g c c g a a c g a c c g a g c g c a g c g a g t c a g t g a g c g a g g a a g c g g a a c t a t g g c g a g c g g c g t c g g c t t g c t g g c t c g c g t c g c t c a g t c a c t c g c t c c t t c g c c t t

240 g a g c g c c c a a t a c g c a a a c c g c c t c t c c c c g c g c g t t g g c c g a t t c a t t a a t g c a g c t g g c t c g c g g g t t a t g c g t t t g g c g g a g a g g g g c g c g c a a c c g g c t a a g t a a t t a c g t c g a c c

300 CAP binding site c a c g a c a g g t t t c c c g a c t g g a a a g c g g g c a g t g a g c g c a a c g c a a t t a a t g t g a g t t a g g t g c t g t c c a a a g g g c t g a c c t t t c g c c c g t c a c t c g c g t t g c g t t a a t t a c a c t c a a t c

360 -35 -10 CAP binding site lac promoter c t c a c t c a t t a g g c a c c c c a g g c t t t a c a c t t t a t g c t t c c g g c t c g t a t g t t g t g t g g a g a g t g a g t a a t c c g t g g g g t c c g a a a t g t g a a a t a c g a a g g c c g a g c a t a c a a c a c a c c t

420 lac operator M13 rev A G C G G A T A A C A A T T T C A C A C A G G M13/pUC Reverse C A G G A A A C A G C T A T G A C M13 Reverse a t t g t g a g c g g a t a a c a a t t t c a c a c a g g a a a c a g c t a t g a c c a t g a t t a c g c c a a g c g c t a a c a c t c g c c t a t t g t t a a a g t g t g t c c t t t g t c g a t a c t g g t a c t a a t g c g g t t c g c g

480 T3 promoter RSV promoter G C A A T T A A C C C T C A C T A A A G G T3 g c a a t t a a c c c t c a c t a a a g g g a a c a a a a g c t g g a g c t g c a a g c t t a a t g t a g t c t t a t g c g t t a a t t g g g a g t g a t t t c c c t t g t t t t c g a c c t c g a c g t t c g a a t t a c a t c a g a a t a c

540 RSV promoter c a a t a c t c t t g t a g t c t t g c a a c a t g g t a a c g a t g a g t t a g c a a c a t g c c t t a c a a g g a g g t t a t g a g a a c a t c a g a a c g t t g t a c c a t t g c t a c t c a a t c g t t g t a c g g a a t g t t c c t c

600 RSV promoter a g a a a a a g c a c c g t g c a t g c c g a t t g g t g g a a g t a a g g t g g t a c g a t c g t g c c t t a t t a g t c t t t t t c g t g g c a c g t a c g g c t a a c c a c c t t c a t t c c a c c a t g c t a g c a c g g a a t a a t c

660 RSV promoter g a a g g c a a c a g a c g g g t c t g a c a t g g a t t g g a c g a a c c a c t g a a t t g c c g c a t t g c a g a g c t t c c g t t g t c t g c c c a g a c t g t a c c t a a c c t g c t t g g t g a c t t a a c g g c g t a a c g t c t c

720 RSV promoter 5' LTR (truncated) a t a t t g t a t t t a a g t g c c t a g c t c g a t a c a t a a a c g g g t c t c t c t g g t t a g a c c a g a t c t t a t a a c a t a a a t t c a c g g a t c g a g c t a t g t a t t t g c c c a g a g a g a c c a a t c t g g t c t a g a

780 5' LTR (truncated) g a g c c t g g g a g c t c t c t g g c t a a c t a g g g a a c c c a c t g c t t a a g c c t c a a t a a a g c t t g c c t c g g a c c c t c g a g a g a c c g a t t g a t c c c t t g g g t g a c g a a t t c g g a g t t a t t t c g a a c g

840 5' LTR (truncated) c t t g a g t g c t t c a a g t a g t g t g t g c c c g t c t g t t g t g t g a c t c t g g t a a c t a g a g a t c c c g a a c t c a c g a a g t t c a t c a c a c a c g g g c a g a c a a c a c a c t g a g a c c a t t g a t c t c t a g g g

900 5' LTR (truncated) t c a g a c c c t t t t a g t c a g t g t g g a a a a t c t c t a g c a g t g g c g c c c g a a c a g g g a c t t g a a a g t c t g g g a a a a t c a g t c a c a c c t t t t a g a g a t c g t c a c c g c g g g c t t g t c c c t g a a c t t

960 HIV-1 Ψ a g c g a a a g g g a a a c c a g a g g a g c t c t c t c g a c g c a g g a c t c g g c t t g c t g a a g c g c g c a c t c g c t t t c c c t t t g g t c t c c t c g a g a g a g c t g c g t c c t g a g c c g a a c g a c t t c g c g c g t g

1020 HIV-1 Ψ g g c a a g a g g c g a g g g g c g g c g a c t g g t g a g t a c g c c a a a a a t t t t g a c t a g c g g a g g c t a c c g t t c t c c g c t c c c c g c c g c t g a c c a c t c a t g c g g t t t t t a a a a c t g a t c g c c t c c g a t

NruI* 1080 HIV-1 Ψ g a a g g a g a g a g a t g g g t g c g a g a g c g t c a g t a t t a a g c g g g g g a g a a t t a g a t c g c g a t g c t t c c t c t c t c t a c c c a c g c t c t c g c a g t c a t a a t t c g c c c c c t c t t a a t c t a g c g c t a c

1140 g g a a a a a a t t c g g t t a a g g c c a g g g g g a a a g a a a a a a t a t a a a t t a a a a c a t a t a g t a t g c c t t t t t t a a g c c a a t t c c g g t c c c c c t t t c t t t t t t a t a t t t a a t t t t g t a t a t c a t a c

1200 g g c a a g c a g g g a g c t a g a a c g a t t c g c a g t t a a t c c t g g c c t g t t a g a a a c a t c a g a a g g c c g t t c g t c c c t c g a t c t t g c t a a g c g t c a a t t a g g a c c g g a c a a t c t t t g t a g t c t t c c

1260 c t g t a g a c a a a t a c t g g g a c a g c t a c a a c c a t c c c t t c a g a c a g g a t c a g a a g a a c t t a g g a c a t c t g t t t a t g a c c c t g t c g a t g t t g g t a g g g a a g t c t g t c c t a g t c t t c t t g a a t c

1320 a t c a t t a t a t a a t a c a g t a g c a a c c c t c t a t t g t g t g c a t c a a a g g a t a g a g a t a a a a g a t a g t a a t a t a t t a t g t c a t c g t t g g g a g a t a a c a c a c g t a g t t t c c t a t c t c t a t t t t c t

1380 c a c c a a g g a a g c t t t a g a c a a g a t a g a g g a a g a g c a a a a c a a a a g t a a g a c c a c c g c a c a g t g g t t c c t t c g a a a t c t g t t c t a t c t c c t t c t c g t t t t g t t t t c a t t c t g g t g g c g t g t

MfeI 1440 MRDNWRS g c a a g c a g c a g c t g a t c t t c a g a c c t g g a g g a g g a g a t a t g a g g g a c a a t t g g a g a a g t g c g t t c g t c g t c g a c t a g a a g t c t g g a c c t c c t c c t c t a t a c t c c c t g t t a a c c t c t t c a c

1500 ELYKYKVVKIEPLGVAPTKA a a t t a t a t a a a t a t a a a g t a g t a a a a a t t g a a c c a t t a g g a g t a g c a c c c a c c a a g g c a a t t a a t a t a t t t a t a t t t c a t c a t t t t t a a c t t g g t a a t c c t c a t c g t g g g t g g t t c c g t t

1560 RRE KRRVVQREKRAVGIGALFLG a g a g a a g a g t g g t g c a g a g a g a a a a a a g a g c a g t g g g a a t a g g a g c t t t g t t c c t t g g g t t c t c t t c t c a c c a c g t c t c t c t t t t t t c t c g t c a c c c t t a t c c t c g a a a c a a g g a a c c c a

1620 RRE FLGAAGSTMGAASMTLTVQA t c t t g g g a g c a g c a g g a a g c a c t a t g g g c g c a g c g t c a a t g a c g c t g a c g g t a c a g g c c a a g a a c c c t c g t c g t c c t t c g t g a t a c c c g c g t c g c a g t t a c t g c g a c t g c c a t g t c c g g t

1680 RRE RQLLSGIVQQQNNLLRAIEA g a c a a t t a t t g t c t g g t a t a g t g c a g c a g c a g a a c a a t t t g c t g a g g g c t a t t g a g g c g c c t g t t a a t a a c a g a c c a t a t c a c g t c g t c g t c t t g t t a a a c g a c t c c c g a t a a c t c c g c g

1740 RRE QQHLLQLTVWGIKQLQARIL a a c a g c a t c t g t t g c a a c t c a c a g t c t g g g g c a t c a a g c a g c t c c a g g c a a g a a t c c t g g t t g t c g t a g a c a a c g t t g a g t g t c a g a c c c c g t a g t t c g t c g a g g t c c g t t c t t a g g a c c

1800 RRE AVERYLKDQQLLGIWGCSGK c t g t g g a a a g a t a c c t a a a g g a t c a a c a g c t c c t g g g g a t t t g g g g t t g c t c t g g a a a a c g a c a c c t t t c t a t g g a t t t c c t a g t t g t c g a g g a c c c c t a a a c c c c a a c g a g a c c t t t t g

AleI 1860 LICTTAVPWNASWSNKSLEQ t c a t t t g c a c c a c t g c t g t g c c t t g g a a t g c t a g t t g g a g t a a t a a a t c t c t g g a a c a g a a g t a a a c g t g g t g a c g a c a c g g a a c c t t a c g a t c a a c c t c a t t a t t t a g a g a c c t t g t c t

1920 IWNHTTWMEWDREINNYTSL t t t g g a a t c a c a c g a c c t g g a t g g a g t g g g a c a g a g a a a t t a a c a a t t a c a c a a g c t t a a a a a c c t t a g t g t g c t g g a c c t a c c t c a c c c t g t c t c t t t a a t t g t t a a t g t g t t c g a a t t

1980 1 5 KNEQELL gp41 peptide IHSLIEESQNQQEKNEQELL t a c a c t c c t t a a t t g a a g a a t c g c a a a a c c a g c a a g a a a a g a a t g a a c a a g a a t t a t t g g a t g t g a g g a a t t a a c t t c t t a g c g t t t t g g t c g t t c t t t t c t t a c t t g t t c t t a a t a a c c

2040 10 15 ELDKWASL (in frame with gp41 peptide) WNWFNITNWLWY gp41 peptide ELDKWASLWNWFNITNWLWY a a t t a g a t a a a t g g g c a a g t t t g t g g a a t t g g t t t a a c a t a a c a a a t t g g c t g t g g t a t a t t a a t c t a t t t a c c c g t t c a a a c a c c t t a a c c a a a t t g t a t t g t t t a a c c g a c a c c a t a t

2100 (in frame with gp41 peptide) IKLFIMIVGGLVGLRIVFAV IKLFIMIVGGLVGLRIVFAV t a a a a t t a t t c a t a a t g a t a g t a g g a g g c t t g g t a g g t t t a a g a a t a g t t t t t g c t g t a c a t t t t a a t a a g t a t t a c t a t c a t c c t c c g a a c c a t c c a a a t t c t t a t c a a a a a c g a c a t g

2160 (in frame with gp41 peptide) LSIVNRVRQGYSPLSFQTHL LSIVNRVRQGYSPLSFQTHL t t t c t a t a g t g a a t a g a g t t a g g c a g g g a t a t t c a c c a t t a t c g t t t c a g a c c c a c c t c c a a a g a t a t c a c t t a t c t c a a t c c g t c c c t a t a a g t g g t a a t a g c a a a g t c t g g g t g g a g g

2220 (in frame with gp41 peptide) PTPRGPDRPEGIEEEGGERD PTPRGPDRPEGIEEEGGERD c a a c c c c g a g g g g a c c c g a c a g g c c c g a a g g a a t a g a a g a a g a a g g t g g a g a g a g a g a c a g t t g g g g c t c c c c t g g g c t g t c c g g g c t t c c t t a t c t t c t t c t t c c a c c t c t c t c t c t g t

BspDI*ClaI* 2280 (in frame with gp41 peptide) RDRSIRLVNGSRRYRSRD* RDRSIRLVNGSRRYRSRD* g a g a c a g a t c c a t t c g a t t a g t g a a c g g a t c t c g a c g g t a t c g a t c a c g a g a c t a g c c t c c t c t g t c t a g g t a a g c t a a t c a c t t g c c t a g a g c t g c c a t a g c t a g t g c t c t g a t c g g a g

2340 UbC promoter g a g c g g g a t c t g g c c t c c g c g c c g g g t t t t g g c g c c t c c c g c g g g c g c c c c c c t c c t c a c c t c g c c c t a g a c c g g a g g c g c g g c c c a a a a c c g c g g a g g g c g c c c g c g g g g g g a g g a g t g

2400 UbC promoter g g c g a g c g c t g c c a c g t c a g a c g a a g g g c g c a g c g a g c g t c c t g a t c c t t c c g c c c g g a c c c g c t c g c g a c g g t g c a g t c t g c t t c c c g c g t c g c t c g c a g g a c t a g g a a g g c g g g c c t g

2460 UbC promoter g c t c a g g a c a g c g g c c c g c t g c t c a t a a g a c t c g g c c t t a g a a c c c c a g t a t c a g c a g a a c g a g t c c t g t c g c c g g g c g a c g a g t a t t c t g a g c c g g a a t c t t g g g g t c a t a g t c g t c t t

2520 UbC promoter g g a c a t t t t a g g a c g g g a c t t g g g t g a c t c t a g g g c a c t g g t t t t c t t t c c a g a g a g c g g c c t g t a a a a t c c t g c c c t g a a c c c a c t g a g a t c c c g t g a c c a a a a g a a a g g t c t c t c g c c

2580 UbC promoter a a c a g g c g a g g a a a a g t a g t c c c t t c t c g g c g a t t c t g c g g a g g g a t c t c c g t g g g g c g g t t g t c c g c t c c t t t t c a t c a g g g a a g a g c c g c t a a g a c g c c t c c c t a g a g g c a c c c c g c c

2640 UbC promoter t g a a c g c c g a t g a t t a t a t a a g g a c g c g c c g g g t g t g g c a c a g c t a g t t c c g t c g c a g c c a c t t g c g g c t a c t a a t a t a t t c c t g c g c g g c c c a c a c c g t g t c g a t c a a g g c a g c g t c g g

2700 UbC promoter g g g a t t t g g g t c g c g g t t c t t g t t t g t g g a t c g c t g t g a t c g t c a c t t g g t g a g t a g c g g c c c t a a a c c c a g c g c c a a g a a c a a a c a c c t a g c g a c a c t a g c a g t g a a c c a c t c a t c g c c

2760 UbC promoter g c t g c t g g g c t g g c c g g g g c t t t c g t g g c c g c c g g g c c g c t c g g t g g g a c g g a a g c g t g t c g a c g a c c c g a c c g g c c c c g a a a g c a c c g g c g g c c c g g c g a g c c a c c c t g c c t t c g c a c a

2820 UbC promoter g g a g a g a c c g c c a a g g g c t g t a g t c t g g g t c c g c g a g c a a g g t t g c c c t g a a c t g g g g g t c c t c t c t g g c g g t t c c c g a c a t c a g a c c c a g g c g c t c g t t c c a a c g g g a c t t g a c c c c c a

2880 UbC promoter t g g g g g g a g c g c a g c a a a a t g g c g g c t g t t c c c g a g t c t t g a a t g g a a g a c g c t t g t g a g a c c c c c c t c g c g t c g t t t t a c c g c c g a c a a g g g c t c a g a a c t t a c c t t c t g c g a a c a c t c

2940 UbC promoter g c g g g c t g t g a g g t c g t t g a a a c a a g g t g g g g g g c a t g g t g g g c g g c a a g a a c c c a a g g t c g c c c g a c a c t c c a g c a a c t t t g t t c c a c c c c c c g t a c c a c c c g c c g t t c t t g g g t t c c a

3000 UbC promoter c t t g a g g c c t t c g c t a a t g c g g g a a a g c t c t t a t t c g g g t g a g a t g g g c t g g g g c a c c a t g a a c t c c g g a a g c g a t t a c g c c c t t t c g a g a a t a a g c c c a c t c t a c c c g a c c c c g t g g t a

3060 UbC promoter c t g g g g a c c c t g a c g t g a a g t t t g t c a c t g a c t g g a g a a c t c g g t t t g t c g t c t g t t g c g g a c c c c t g g g a c t g c a c t t c a a a c a g t g a c t g a c c t c t t g a g c c a a a c a g c a g a c a a c g c

3120 UbC promoter g g g g c g g c a g t t a t g g c g g t g c c g t t g g g c a g t g c a c c c g t a c c t t t g g g a g c g c g c g c c c c c c g c c g t c a a t a c c g c c a c g g c a a c c c g t c a c g t g g g c a t g g a a a c c c t c g c g c g c g g

3180 UbC promoter c t c g t c g t g t c g t g a c g t c a c c c g t t c t g t t g g c t t a t a a t g c a g g g t g g g g c c a c c t g c g a g c a g c a c a g c a c t g c a g t g g g c a a g a c a a c c g a a t a t t a c g t c c c a c c c c g g t g g a c g

3240 UbC promoter c g g t a g g t g t g c g g t a g g c t t t t c t c c g t c g c a g g a c g c a g g g t t c g g g c c t a g g g t a g g g c c a t c c a c a c g c c a t c c g a a a a g a g g c a g c g t c c t g c g t c c c a a g c c c g g a t c c c a t c c

3300 UbC promoter c t c t c c t g a a t c g a c a g g c g c c g g a c c t c t g g t g a g g g g a g g g a t a a g t g a g g c g t c a g t g a g a g g a c t t a g c t g t c c g c g g c c t g g a g a c c a c t c c c c t c c c t a t t c a c t c c g c a g t c a

3360 UbC promoter T G A A G C T C C G G T T T T G A A C T hUBCpro-F t t c t t t g g t c g g t t t t a t g t a c c t a t c t t c t t a a g t a g c t g a a g c t c c g g t t t t g a a c t a a a g a a a c c a g c c a a a a t a c a t g g a t a g a a g a a t t c a t c g a c t t c g a g g c c a a a a c t t g a t

3420 UbC promoter t g c g c t c g g g g t t g g c g a g t g t g t t t t g t g a a g t t t t t t a g g c a c c t t t t g a a a t g t a a t a c g c g a g c c c c a a c c g c t c a c a c a a a a c a c t t c a a a a a a t c c g t g g a a a a c t t t a c a t t a

3480 UbC promoter c a t t t g g g t c a a t a t g t a a t t t t c a g t g t t a g a c t a g t a a a t t g t c c g c t a a a t t c t g g c g t a a a c c c a g t t a t a c a t t a a a a g t c a c a a t c t g a t c a t t t a a c a g g c g a t t t a a g a c c g

AgeIBmtINheI 3540 1 YP UbC promoter Kozak sequence HA MGYP c g t t t t t g g c t t t t t t g t t a g a c g a a g c g c t a g c g c t a c c g g t g c c a c c a t g g g c t a c c c g c a a a a a c c g a a a a a a c a a t c t g c t t c g c g a t c g c g a t g g c c a c g g t g g t a c c c g a t g g g

SalI 3600 5 YDVPDYA (in frame with HA) DLNGGGGGSTMRT HA YDVPDYADLNGGGGGSTMRT c t a t g a t g t g c c t g a c t a c g c a g a t c t c a a t g g t g g t g g t g g t g g g t c g a c g a t g a g a a c g a t a c t a c a c g g a c t g a t g c g t c t a g a g t t a c c a c c a c c a c c a c c c a g c t g c t a c t c t t g

3660 (in frame with HA) TKVYKLVIHKKGFGGSDDEL TKVYKLVIHKKGFGGSDDEL a a c a a a g g t c t a c a a a c t c g t c a t c c a c a a g a a g g g c t t t g g g g g c a g t g a t g a t g a g c t t t g t t t c c a g a t g t t t g a g c a g t a g g t g t t c t t c c c g a a a c c c c c g t c a c t a c t a c t c g a

3720 (in frame with HA) VVNPKVFPHIKLGDIVEIAH VVNPKVFPHIKLGDIVEIAH a g t t g t g a a c c c c a a a g t g t t c c c t c a c a t c a a g c t t g g a g a c a t t g t a g a g a t t g c a c a t c a a c a c t t g g g g t t t c a c a a g g g a g t g t a g t t c g a a c c t c t g t a a c a t c t c t a a c g t g t

3780 (in frame with HA) PNDEYSPLLLQVKSLKEDLQ PNDEYSPLLLQVKSLKEDLQ c c c c a a c g a t g a a t a c a g c c c t c t g c t t t t g c a g g t c a a g t c t c t t a a g g a a g a t t t a c a g g g g t t g c t a c t t a t g t c g g g a g a c g a a a a c g t c c a g t t c a g a g a a t t c c t t c t a a a t g t

3840 (in frame with HA) KETISVDQTVTQVFRLRPYQ KETISVDQTVTQVFRLRPYQ g a a g g a a a c t a t c a g t g t g g a c c a g a c t g t g a c t c a a g t g t t c c g g c t g a g a c c t t a t c a c t t c c t t t g a t a g t c a c a c c t g g t c t g a c a c t g a g t t c a c a a g g c c g a c t c t g g a a t a g t

3900 (in frame with HA) DVYVNVVDPKDVTLDLVELT DVYVNVVDPKDVTLDLVELT g g a t g t c t a t g t t a a t g t c g t a g a c c c t a a g g a t g t g a c c c t t g a c c t a g t g g a a t t a a c c c t a c a g a t a c a a t t a c a g c a t c t g g g a t t c c t a c a c t g g g a a c t g g a t c a c c t t a a t t g

3960 (in frame with HA) FKDQYIGRGDMWRLKKSLVS FKDQYIGRGDMWRLKKSLVS t t t t a a g g a t c a g t a t a t t g g c c g t g g g g a t a t g t g g c g a c t a a a g a a a a g t t t g g t c a g a a a a t t c c t a g t c a t a t a a c c g g c a c c c c t a t a c a c c g c t g a t t t c t t t t c a a a c c a g t c

4020 (in frame with HA) TCAYITQKVEFAGIRAQAGE TCAYITQKVEFAGIRAQAGE c a c a t g t g c c t a t a t c a c c c a g a a g g t g g a g t t t g c t g g c a t c a g a g c a c a g g c t g g t g a g t g t a c a c g g a t a t a g t g g g t c t t c c a c c t c a a a c g a c c g t a g t c t c g t g t c c g a c c a c t

4080 (in frame with HA) LWVKNEKVMCGYISEDTRVV LWVKNEKVMCGYISEDTRVV a c t g t g g g t t a a g a a t g a g a a g g t c a t g t g t g g c t a c a t c a g t g a a g a t a c c a g g g t g g t t g a c a c c c a a t t c t t a c t c t t c c a g t a c a c a c c g a t g t a g t c a c t t c t a t g g t c c c a c c a

4140 (in frame with HA) FRSTSAMVYIFIQMSCEMWD FRSTSAMVYIFIQMSCEMWD g t t t c g t t c t a c g t c g g c t a t g g t t t a c a t a t t t a t t c a g a t g a g c t g t g a a a t g t g g g a c a a a g c a a g a t g c a g c c g a t a c c a a a t g t a t a a a t a a g t c t a c t c g a c a c t t t a c a c c c t

4200 (in frame with HA) FDIYGDLYFEKAVNGFLADL FDIYGDLYFEKAVNGFLADL t t t t g a t a t t t a t g g g g a t t t g t a t t t t g a g a a a g c t g t g a a t g g t t t c c t t g c t g a t c t a a a a c t a t a a a t a c c c c t a a a c a t a a a a c t c t t t c g a c a c t t a c c a a a g g a a c g a c t a g a

4260 (in frame with HA) FTKWKEKNCSHEVTVVLFSR FTKWKEKNCSHEVTVVLFSR a t t t a c c a a g t g g a a g g a g a a g a a c t g t a g t c a t g a a g t g a c a g t g g t c c t g t t t t c t a g t a a a t g g t t c a c c t t c c t c t t c t t g a c a t c a g t a c t t c a c t g t c a c c a g g a c a a a a g a t c

4320 (in frame with HA) TFYDAKSVDEFPEINRASIR TFYDAKSVDEFPEINRASIR a a c t t t c t a t g a t g c a a a a t c t g t t g a t g a a t t t c c t g a a a t a a a c c g a g c c t c a a t t c g t t g a a a g a t a c t a c g t t t t a g a c a a c t a c t t a a a g g a c t t t a t t t g g c t c g g a g t t a a g c

4380 (in frame with HA) QDHKGRFYEDFYKVVVQNER QDHKGRFYEDFYKVVVQNER a c a g g a t c a c a a g g g g a g a t t c t a t g a a g a c t t t t a c a a a g t g g t g g t g c a g a a t g a g a g t g t c c t a g t g t t c c c c t c t a a g a t a c t t c t g a a a a t g t t t c a c c a c c a c g t c t t a c t c t c

4440 (in frame with HA) REEWTSLLVTIKKLFIQYPV REEWTSLLVTIKKLFIQYPV a a g a g a a g a a t g g a c t t c a c t t c t c g t a a c c a t t a a a a a a c t c t t c a t c c a g t a t c c a g t t t c t c t t c t t a c c t g a a g t g a a g a g c a t t g g t a a t t t t t t g a g a a g t a g g t c a t a g g t c a

4500 (in frame with HA) LVRLEQAEGFPQGDNSTSAQ LVRLEQAEGFPQGDNSTSAQ g t t g g t g c g a c t g g a a c a g g c a g a g g g c t t t c c t c a a g g a g a t a a t t c t a c c t c a g c a c a c a a c c a c g c t g a c c t t g t c c g t c t c c c g a a a g g a g t t c c t c t a t t a a g a t g g a g t c g t g t

4560 (in frame with HA) GNYLEAINLSFNVFDKHYIN GNYLEAINLSFNVFDKHYIN a g g a a a c t a c c t g g a g g c c a t c a a t c t g t c a t t c a a t g t g t t t g a t a a g c a c t a c a t c a a t c c t t t g a t g g a c c t c c g g t a g t t a g a c a g t a a g t t a c a c a a a c t a t t c g t g a t g t a g t t

4620 (in frame with HA) RNFDRTGQMSVVITPGVGVF RNFDRTGQMSVVITPGVGVF c c g c a a c t t t g a c c g a a c t g g g c a g a t g t c a g t g g t g a t c a c g c c c g g g g t g g g t g t c t t g g c g t t g a a a c t g g c t t g a c c c g t c t a c a g t c a c c a c t a g t g c g g g c c c c a c c c a c a g a a

4680 (in frame with HA) EVDRLLMILTKQRMIDNGIG EVDRLLMILTKQRMIDNGIG t g a a g t g g a c c g c c t a c t c a t g a t c c t g a c c a a g c a g c g g a t g a t a g a t a a t g g a a t t g g a c t t c a c c t g g c g g a t g a g t a c t a g g a c t g g t t c g t c g c c t a c t a t c t a t t a c c t t a a c c

4740 (in frame with HA) VDLVCMGEQPLHAVPLFKLH VDLVCMGEQPLHAVPLFKLH t g t g g a t t t g g t g t g c a t g g g a g a g c a a c c g t t a c a t g c t g t c c c a t t g t t c a a g c t c c a a c a c c t a a a c c a c a c g t a c c c t c t c g t t g g c a a t g t a c g a c a g g g t a a c a a g t t c g a g g t

4800 (in frame with HA) NRSAPRDSRLGDDYNIPHWI NRSAPRDSRLGDDYNIPHWI t a a t c g g a g t g c t c c c c g t g a t t c t c g t c t g g g c g a t g a c t a t a a t a t c c c t c a c t g g a t a t t a g c c t c a c g a g g g g c a c t a a g a g c a g a c c c g c t a c t g a t a t t a t a g g g a g t g a c c t a

4860 (in frame with HA) NHSFYTSKSQLFCNSFTPRI NHSFYTSKSQLFCNSFTPRI a a a c c a c a g t t t c t a c a c a t c c a a a a g c c a g c t c t t t t g t a a t a g t t t c a c c c c a c g a a t t t t g g t g t c a a a g a t g t g t a g g t t t t c g g t c g a g a a a a c a t t a t c a a a g t g g g g t g c t t a

4920 (in frame with HA) KLAGKKPASEKAKNGRDTSL KLAGKKPASEKAKNGRDTSL a a a a c t g g c a g g a a a g a a g c c c g c c t c t g a g a a a g c a a a a a a t g g c c g t g a t a c a t c t c t t t t t g a c c g t c c t t t c t t c g g g c g g a g a c t c t t t c g t t t t t t a c c g g c a c t a t g t a g a g a

4980 (in frame with HA) GSPKESENALPIQVDYDAYD GSPKESENALPIQVDYDAYD c g g g a g t c c a a a a g a a t c t g a g a a c g c c c t t c c c a t c c a a g t a g a t t a t g a c g c c t a t g a g c c c t c a g g t t t t c t t a g a c t c t t g c g g g a a g g g t a g g t t c a t c t a a t a c t g c g g a t a c t

5040 (in frame with HA) AQVFRLPGPSRAQCLTTCRS AQVFRLPGPSRAQCLTTCRS c g c t c a a g t g t t c a g g c t g c c c g g c c c a t c c c g g g c c c a g t g c c t c a c c a c c t g c a g a t c g c g a g t t c a c a a g t c c g a c g g g c c g g g t a g g g c c c g g g t c a c g g a g t g g t g g a c g t c t a g

5100 (in frame with HA) VRERESHSRKSASSCDVSSS VRERESHSRKSASSCDVSSS t g t g c g a g a g c g a g a g a g t c a c a g t c g a a a g a g t g c c a g c t c c t g t g a t g t t t c a t c c a g a c a c g c t c t c g c t c t c t c a g t g t c a g c t t t c t c a c g g t c g a g g a c a c t a c a a a g t a g g t c

5160 (in frame with HA) PSLPSRTLPTEEVRSQASDD PSLPSRTLPTEEVRSQASDD c c c t t c c c t a c c a a g c c g c a c a c t g c c c a c t g a g g a a g t g a g g a g c c a g g c t t c t g a c g a g g g a a g g g a t g g t t c g g c g t g t g a c g g g t g a c t c c t t c a c t c c t c g g t c c g a a g a c t g c t

5220 (in frame with HA) SSLGKSANILMIPHPHLHQY SSLGKSANILMIPHPHLHQY c a g c t c c c t a g g c a a g a g t g c c a a c a t c c t g a t g a t c c c a c a c c c c c a c c t g c a c c a g t a g t c g a g g g a t c c g t t c t c a c g g t t g t a g g a c t a c t a g g g t g t g g g g g t g g a c g t g g t c a t

5280 (in frame with HA) EVSSSLGYTSTRDVLENMME EVSSSLGYTSTRDVLENMME t g a a g t c a g c a g c t c c t t g g g a t a c a c c a g c a c t c g a g a t g t c c t g g a g a a c a t g a t g g a a c t t c a g t c g t c g a g g a a c c c t a t g t g g t c g t g a g c t c t a c a g g a c c t c t t g t a c t a c c t

5340 (in frame with HA) PPQRDSSAPGRFHVGSAESM PPQRDSSAPGRFHVGSAESM g c c a c c a c a g c g a g a c t c c a g t g c a c c a g g g a g g t t t c a c g t t g g c a g t g c a g a a t c c a t c g g t g g t g t c g c t c t g a g g t c a c g t g g t c c c t c c a a a g t g c a a c c g t c a c g t c t t a g g t a

5400 (in frame with HA) LHVRPGGYTPQRALINPFAP LHVRPGGYTPQRALINPFAP g c t g c a t g t t c g a c c t g g t g g a t a c a c g c c c c a g a g a g c a c t g a t t a a c c c c t t c g c t c c c g a c g t a c a a g c t g g a c c a c c t a t g t g c g g g g t c t c t c g t g a c t a a t t g g g g a a g c g a g g

5460 (in frame with HA) SRMPMKLTSNRRRWMHTFPV SRMPMKLTSNRRRWMHTFPV c t c t c g g a t g c c c a t g a a g c t t a c g t c c a a c a g a a g g c g c t g g a t g c a c a c t t t t c c t g t g a g a g c c t a c g g g t a c t t c g a a t g c a g g t t g t c t t c c g c g a c c t a c g t g t g a a a a g g a c a

5520 (in frame with HA) GPSGEAIQIHHQTRQNMAEL GPSGEAIQIHHQTRQNMAEL g g g g c c a t c c g g a g a a g c c a t c c a g a t c c a c c a c c a g a c c c g a c a g a a t a t g g c g g a g c t c c c c g g t a g g c c t c t t c g g t a g g t c t a g g t g g t g g t c t g g g c t g t c t t a t a c c g c c t c g a

5580 (in frame with HA) QGSGQRDPTHSSAELLELAY QGSGQRDPTHSSAELLELAY a c a a g g c a g c g g g c a g a g g g a t c c a a c t c a c t c c t c t g c a g a g c t g c t g g a g t t a g c a t a t g t t c c g t c g c c c g t c t c c c t a g g t t g a g t g a g g a g a c g t c t c g a c g a c c t c a a t c g t a t

5640 (in frame with HA) HEAAGRHSNSRQPGDGMSFL HEAAGRHSNSRQPGDGMSFL t c a t g a a g c t g c t g g a a g g c a c a g c a a t t c c c g c c a g c c t g g t g a c g g c a t g t c c t t c t t a g t a c t t c g a c g a c c t t c c g t g t c g t t a a g g g c g g t c g g a c c a c t g c c g t a c a g g a a g a a

5700 (in frame with HA) NFSGTEELSVGLLSNSGAGM NFSGTEELSVGLLSNSGAGM g a a c t t c a g t g g a a c a g a g g a g c t t t c t g t c g g c c t g c t t a g c a a c a g t g g t g c a g g t a t c t t g a a g t c a c c t t g t c t c c t c g a a a g a c a g c c g g a c g a a t c g t t g t c a c c a c g t c c a t a

5760 (in frame with HA) NPRTQNKDSLEDSVSTSPDP NPRTQNKDSLEDSVSTSPDP g a a t c c t a g g a c c c a g a a t a a g g a t t c t c t a g a g g a c a g t g t t t c t a c c t c t c c a g a c c c c t t a g g a t c c t g g g t c t t a t t c c t a a g a g a t c t c c t g t c a c a a a g a t g g a g a g g t c t g g g

5820 (in frame with HA) ILTLSAPPVVPGFCCTVGVD ILTLSAPPVVPGFCCTVGVD a a t t c t g a c a c t g t c t g c t c c c c c t g t a g t g c c a g g c t t c t g t t g c a c a g t t g g a g t g g a t t a a g a c t g t g a c a g a c g a g g g g g a c a t c a c g g t c c g a a g a c a a c g t g t c a a c c t c a c c t

5880 (in frame with HA) WKSLTTPACLPLTTDYFPDR WKSLTTPACLPLTTDYFPDR c t g g a a g t c t c t c a c t a c t c c g g c g t g c c t c c c c c t t a c c a c c g a c t a c t t c c c t g a c c g g a c c t t c a g a g a g t g a t g a g g c c g c a c g g a g g g g g a a t g g t g g c t g a t g a a g g g a c t g g c

5940 (in frame with HA) QGLQNDYTEGCYDLLPEADI QGLQNDYTEGCYDLLPEADI c c a g g g c c t g c a g a a t g a c t a c a c a g a g g g c t g t t a t g a t c t c c t t c c a g a a g c a g a c a t g g t c c c g g a c g t c t t a c t g a t g t g t c t c c c g a c a a t a c t a g a g g a a g g t c t t c g t c t g t a

6000 (in frame with HA) DRRDEDGVQMTAQQVFEEFI DRRDEDGVQMTAQQVFEEFI c g a c a g g a g g g a c g a a g a t g g t g t g c a g a t g a c a g c c c a g c a g g t a t t t g a a g a g t t t a t g c t g t c c t c c c t g c t t c t a c c a c a c g t c t a c t g t c g g g t c g t c c a t a a a c t t c t c a a a t a

6060 (in frame with HA) CQRLMQGYQIIVQPKTQKPN CQRLMQGYQIIVQPKTQKPN t t g c c a a c g t c t c a t g c a g g g c t a c c a a a t c a t a g t g c a g c c c a a g a c a c a g a a a c c c a a a a c g g t t g c a g a g t a c g t c c c g a t g g t t t a g t a t c a c g t c g g g t t c t g t g t c t t t g g g t t

6120 (in frame with HA) PAVPPPLSSSPLYSRGLVSR PAVPPPLSSSPLYSRGLVSR t c c t g c t g t c c c g c c c c c g c t g a g c a g t a g c c c a c t c t a t a g c c g a g g c c t t g t g t c c c g a g g a c g a c a g g g c g g g g g c g a c t c g t c a t c g g g t g a g a t a t c g g c t c c g g a a c a c a g g g c

6180 (in frame with HA) NRPEEEDQYWLSMGRTFHKV NRPEEEDQYWLSMGRTFHKV a a a c c g c c c t g a g g a g g a g g a c c a g t a t t g g c t g a g t a t g g g c a g a a c g t t c c a c a a a g t t t t g g c g g g a c t c c t c c t c c t g g t c a t a a c c g a c t c a t a c c c g t c t t g c a a g g t g t t t c a

6240 (in frame with HA) TLKDKMITVTRYLPKYPYES TLKDKMITVTRYLPKYPYES g a c g c t g a a g g a t a a g a t g a t c a c a g t g a c g c g a t a c c t t c c c a a g t a t c c t t a t g a a t c c t g c g a c t t c c t a t t c t a c t a g t g t c a c t g c g c t a t g g a a g g g t t c a t a g g a a t a c t t a g

6300 (in frame with HA) AQIHYTYSLCPSHSDSEFVS AQIHYTYSLCPSHSDSEFVS t g c c c a g a t c c a c t a c a c c t a c a g c c t c t g t c c t t c c c a c t c a g a c t c a g a g t t c g t c t c a c g g g t c t a g g t g a t g t g g a t g t c g g a g a c a g g a a g g g t g a g t c t g a g t c t c a a g c a g a g

6360 (in frame with HA) CWVEFSHERLEEYKWNYLDQ CWVEFSHERLEEYKWNYLDQ c t g c t g g g t g g a g t t t t c c c a c g a a c g g c t g g a g g a g t a c a a g t g g a a t t a c t t a g a t c a g a c g a c c c a c c t c a a a a g g g t g c t t g c c g a c c t c c t c a t g t t c a c c t t a a t g a a t c t a g t

6420 (in frame with HA) YICSAGSEDFSLIESLKFWR YICSAGSEDFSLIESLKFWR g t a t a t c t g t t c t g c c g g c t c t g a a g a c t t c a g c t t a a t t g a g t c c c t g a a g t t c t g g a g c a t a t a g a c a a g a c g g c c g a g a c t t c t g a a g t c g a a t t a a c t c a g g g a c t t c a a g a c c t c

6480 (in frame with HA) TRFLLLPACVTATKRITEGE TRFLLLPACVTATKRITEGE g a c c c g c t t c c t g c t g c t g c c a g c c t g t g t c a c c g c c a c c a a g c g c a t c a c g g a g g g g g a c t g g g c g a a g g a c g a c g a c g g t c g g a c a c a g t g g c g g t g g t t c g c g t a g t g c c t c c c c c t

6540 (in frame with HA) AHCDIYGDRPRADEDEWQLL AHCDIYGDRPRADEDEWQLL g g c c c a c t g c g a c a t c t a t g g g g a c a g g c c c c g t g c a g a c g a g g a c g a g t g g c a a c t c c t c c g g g t g a c g c t g t a g a t a c c c c t g t c c g g g g c a c g t c t g c t c c t g c t c a c c g t t g a g g a

6600 (in frame with HA) DGFVRFVEGLNRIRRRHRSD DGFVRFVEGLNRIRRRHRSD g g a t g g t t t t g t c c g c t t t g t g g a g g g c t t g a a t c g c a t t c g c a g g c g g c a t c g c t c g g a c c t a c c a a a a c a g g c g a a a c a c c t c c c g a a c t t a g c g t a a g c g t c c g c c g t a g c g a g c c t

6660 (in frame with HA) RMMRKGTAMKGLQMTGPIST RMMRKGTAMKGLQMTGPIST t c g c a t g a t g c g g a a a g g g a c c g c c a t g a a a g g c t t g c a g a t g a c t g g g c c c a t t t c c a c a g c g t a c t a c g c c t t t c c c t g g c g g t a c t t t c c g a a c g t c t a c t g a c c c g g g t a a a g g t g

6720 (in frame with HA) HSLESTAPPVGKKGTSALSA HSLESTAPPVGKKGTSALSA g c a t t c t c t g g a g t c a a c t g c a c c c c c a g t g g g g a a g a a g g g a a c c t c a g c t c t c t c t g c c g t a a g a g a c c t c a g t t g a c g t g g g g g t c a c c c c t t c t t c c c t t g g a g t c g a g a g a g a c g

6780 (in frame with HA) LLEMEASQKCLGEQQAAVHG LLEMEASQKCLGEQQAAVHG c c t g t t g g a g a t g g a g g c c a g t c a g a a g t g c c t g g g a g a a c a g c a g g c a g c t g t g c a t g g g g a c a a c c t c t a c c t c c g g t c a g t c t t c a c g g a c c c t c t t g t c g t c c g t c g a c a c g t a c c

6840 (in frame with HA) GKSSAQSAESSSVAMTPTYM GKSSAQSAESSSVAMTPTYM t g g g a a g a g c t c c g c c c a g t c a g c c g a g a g c a g c a g c g t t g c c a t g a c t c c c a c c t a c a t a c c c t t c t c g a g g c g g g t c a g t c g g c t c t c g t c g t c g c a a c g g t a c t g a g g g t g g a t g t a

6900 (in frame with HA) DSPRKDGAFFMEFVRSPRTA DSPRKDGAFFMEFVRSPRTA g g a c a g c c c a c g a a a g g a c g g g g c c t t c t t t a t g g a g t t t g t c c g c a g c c c a c g c a c a g c c c t g t c g g g t g c t t t c c t g c c c c g g a a g a a a t a c c t c a a a c a g g c g t c g g g t g c g t g t c g

6960 (in frame with HA) SSAFYPQVSVDQTATPMLDG SSAFYPQVSVDQTATPMLDG a t c g t c c g c c t t c t a c c c t c a g g t a t c t g t g g a c c a a a c a g c c a c t c c t a t g t t g g a c g g t a g c a g g c g g a a g a t g g g a g t c c a t a g a c a c c t g g t t t g t c g g t g a g g a t a c a a c c t g c c

NdeI 7020 (in frame with HA) TSLGICTGQSMDRGNSQTFG TSLGICTGQSMDRGNSQTFG c a c c a g t t t g g g c a t a t g c a c a g g c c a a t c c a t g g a c a g a g g c a a c a g c c a g a c c t t t g g g t g g t c a a a c c c g t a t a c g t g t c c g g t t a g g t a c c t g t c t c c g t t g t c g g t c t g g a a a c c

7080 (in frame with HA) NSQNIGEQGYSSTNSSDSSS NSQNIGEQGYSSTNSSDSSS g a a c t c c c a g a a c a t a g g a g a a c a g g g c t a c t c c t c c a c a a a c t c c a g t g a c a g c a g c t c c t t g a g g g t c t t g t a t c c t c t t g t c c c g a t g a g g a g g t g t t t g a g g t c a c t g t c g t c g a g

7140 (in frame with HA) QQLVASSLTSSSTLTEILEA QQLVASSLTSSSTLTEILEA t c a g c a g c t g g t g g c a a g c t c c t t g a c c t c a t c c t c t a c c c t g a c a g a g a t c c t g g a a g c a g t c g t c g a c c a c c g t t c g a g g a a c t g g a g t a g g a g a t g g g a c t g t c t c t a g g a c c t t c g

7200 (in frame with HA) MKHPSTGVQLLSEQKGLSPY MKHPSTGVQLLSEQKGLSPY c a t g a a g c a c c c c t c g a c a g g a g t c c a g c t g c t c t c t g a a c a g a a g g g c c t c t c a c c g t a g t a c t t c g t g g g g a g c t g t c c t c a g g t c g a c g a g a g a c t t g t c t t c c c g g a g a g t g g c a t

PmlI 7260 (in frame with HA) CFISAEVVHWLVNHVEGIQT CFISAEVVHWLVNHVEGIQT c t g c t t c a t c a g c g c g g a g g t g g t a c a c t g g t t g g t g a a c c a c g t g g a g g g g a t c c a g a c g a c g a a g t a g t c g c g c c t c c a c c a t g t g a c c a a c c a c t t g g t g c a c c t c c c c t a g g t c t g

7320 (in frame with HA) QAMAIDIMQKMLEEQLITHA QAMAIDIMQKMLEEQLITHA a c a g g c g a t g g c c a t t g a c a t c a t g c a g a a a a t g c t g g a a g a g c a g c t c a t c a c a c a t g c t g t c c g c t a c c g g t a a c t g t a g t a c g t c t t t t a c g a c c t t c t c g t c g a g t a g t g t g t a c g

7380 (in frame with HA) SGEAWRTFIYGFYFYKIVTD SGEAWRTFIYGFYFYKIVTD a t c t g g c g a a g c c t g g c g g a c c t t c a t c t a c g g c t t c t a t t t c t a c a a g a t a g t a a c g g a t a g a c c g c t t c g g a c c g c c t g g a a g t a g a t g c c g a a g a t a a a g a t g t t c t a t c a t t g c c t

7440 (in frame with HA) KEPDRVAMQQPATTWHTAGV KEPDRVAMQQPATTWHTAGV c a a a g a g c c c g a c c g a g t g g c c a t g c a g c a g c c c g c c a c c a c c t g g c a c a c a g c a g g a g t g t t t c t c g g g c t g g c t c a c c g g t a c g t c g t c g g g c g g t g g t g g a c c g t g t g t c g t c c t c a

7500 (in frame with HA) DDFASFQRKWFEVAFVAEEL DDFASFQRKWFEVAFVAEEL g g a c g a c t t c g c c a g c t t c c a g c g c a a g t g g t t t g a g g t g g c c t t t g t g g c a g a a g a g c t c c t g c t g a a g c g g t c g a a g g t c g c g t t c a c c a a a c t c c a c c g g a a a c a c c g t c t t c t c g a

7560 (in frame with HA) VHSEIPAFLLPWLPSRPASY VHSEIPAFLLPWLPSRPASY c g t g c a c t c t g a g a t t c c t g c c t t t c t c c t g c c c t g g c t g c c t a g c c g g c c a g c c t c c t a g c a c g t g a g a c t c t a a g g a c g g a a a g a g g a c g g g a c c g a c g g a t c g g c c g g t c g g a g g a t

7620 (in frame with HA) ASRHSSFSRSFGGRSQAAAL ASRHSSFSRSFGGRSQAAAL t g c a a g t a g g c a c a g c t c c t t t a g c c g a a g t t t t g g a g g a c g g a g c c a g g c g g c a g c a c t a c g t t c a t c c g t g t c g a g g a a a t c g g c t t c a a a a c c t c c t g c c t c g g t c c g c c g t c g t g a

7680 (in frame with HA) LAATVPEQRTVTLDVDVNNR LAATVPEQRTVTLDVDVNNR t t t a g c t g c c a c t g t c c c a g a g c a g a g g a c t g t g a c c c t g g a t g t t g a c g t g a a c a a c c g a a a t c g a c g g t g a c a g g g t c t c g t c t c c t g a c a c t g g g a c c t a c a a c t g c a c t t g t t g g c

7740 (in frame with HA) TDRLEWCSCYYHGNFSLNAA TDRLEWCSCYYHGNFSLNAA c a c a g a c c g g c t g g a g t g g t g c a g c t g t t a t t a c c a t g g c a a c t t t t c t c t g a a t g c a g c g t g t c t g g c c g a c c t c a c c a c g t c g a c a a t a a t g g t a c c g t t g a a a a g a g a c t t a c g t c g

7800 (in frame with HA) FEIKLHWMAVTAAVLFEMVQ FEIKLHWMAVTAAVLFEMVQ c t t t g a g a t c a a g c t g c a c t g g a t g g c g g t g a c c g c a g c a g t a c t c t t c g a g a t g g t c c a g a a a c t c t a g t t c g a c g t g a c c t a c c g c c a c t g g c g t c g t c a t g a g a a g c t c t a c c a g g t

7860 (in frame with HA) GWHRKATSCGFLLVPVLEGP GWHRKATSCGFLLVPVLEGP a g g t t g g c a t c g g a a a g c c a c c t c c t g t g g c t t c t t g t t a g t c c c a g t t t t g g a g g g g c c t c c a a c c g t a g c c t t t c g g t g g a g g a c a c c g a a g a a c a a t c a g g g t c a a a a c c t c c c c g g

7920 (in frame with HA) FALPSYLYGDPLRAQLFIPL FALPSYLYGDPLRAQLFIPL t t t t g c a c t g c c c a g t t a c c t g t a t g g c g a c c c c c t t c g t g c c c a g c t c t t c a t c c c a c t a a a a c g t g a c g g g t c a a t g g a c a t a c c g c t g g g g g a a g c a c g g g t c g a g a a g t a g g g t g a

7980 (in frame with HA) NISCLLKEGSEHLFDSFEPE NISCLLKEGSEHLFDSFEPE c a a c a t c a g c t g c t t g c t c a a g g a g g g c a g c g a g c a c c t g t t t g a t a g c t t t g a a c c c g a g t t g t a g t c g a c g a a c g a g t t c c t c c c g t c g c t c g t g g a c a a a c t a t c g a a a c t t g g g c t

BsrGI 8040 (in frame with HA) TYWDRMHLFQEAIAHRFGFV TYWDRMHLFQEAIAHRFGFV a a c g t a c t g g g a t c g a a t g c a c c t c t t c c a g g a a g c c a t t g c a c a c a g g t t t g g g t t t g t t t g c a t g a c c c t a g c t t a c g t g g a g a a g g t c c t t c g g t a a c g t g t g t c c a a a c c c a a a c a

8100 (in frame with HA) QDKYSASAFNFPAENKPQYI QDKYSASAFNFPAENKPQYI a c a a g a t a a a t a t t c t g c c t c t g c t t t t a a c t t c c c t g c t g a g a a c a a g c c t c a g t a t a t t g t t c t a t t t a t a a g a c g g a g a c g a a a a t t g a a g g g a c g a c t c t t g t t c g g a g t c a t a t a

8160 (in frame with HA) HVTGTVFLQLPYSKRKFSGQ HVTGTVFLQLPYSKRKFSGQ c c a c g t t a c a g g a a c a g t g t t t c t g c a g c t g c c c t a c t c c a a g c g c a a g t t c t c a g g g c a g g t g c a a t g t c c t t g t c a c a a a g a c g t c g a c g g g a t g a g g t t c g c g t t c a a g a g t c c c g t

8220 (in frame with HA) QRRRRNSTSSTNQNMFCEER QRRRRNSTSSTNQNMFCEER g c a g c g g c g g c g g c g g a a c t c c a c c a g c t c c a c c a a c c a g a a c a t g t t c t g c g a g g a g c g c g t c g c c g c c g c c g c c t t g a g g t g g t c g a g g t g g t t g g t c t t g t a c a a g a c g c t c c t c g c

8280 (in frame with HA) VGYNWAYNTMLTKTWRSSAT VGYNWAYNTMLTKTWRSSAT g g t c g g c t a c a a c t g g g c c t a c a a c a c c a t g c t c a c c a a a a c a t g g c g c t c c a g c g c c a c c c a g c c g a t g t t g a c c c g g a t g t t g t g g t a c g a g t g g t t t t g t a c c g c g a g g t c g c g g t g

8340 (in frame with HA) GDEKFADRLLKDFTDFCINR GDEKFADRLLKDFTDFCINR a g g g g a t g a a a a g t t t g c t g a t c g g c t g c t g a a g g a c t t c a c g g a c t t c t g c a t c a a c c g t c c c c t a c t t t t c a a a c g a c t a g c c g a c g a c t t c c t g a a g t g c c t g a a g a c g t a g t t g g c

8400 (in frame with HA) DNRLVTFWTSCLEKMHASAP DNRLVTFWTSCLEKMHASAP t g a c a a c c g g c t g g t c a c g t t c t g g a c a a g t t g c c t g g a g a a g a t g c a t g c c a g t g c c c c a c t g t t g g c c g a c c a g t g c a a g a c c t g t t c a a c g g a c c t c t t c t a c g t a c g g t c a c g g g g

EcoRI 8460 * cPPT/CTS * g t g a g a a t t c t c g a c c t c g a g a c a a a t g g c a g t a t t c a t c c a c a a t t t t a a a a g a a a a g g c a c t c t t a a g a g c t g g a g c t c t g t t t a c c g t c a t a a g t a g g t g t t a a a a t t t t c t t t t c c

8520 cPPT/CTS g g g g a t t g g g g g g t a c a g t g c a g g g g a a a g a a t a g t a g a c a t a a t a g c a a c a g a c a t a c a c c c c t a a c c c c c c a t g t c a c g t c c c c t t t c t t a t c a t c t g t a t t a t c g t t g t c t g t a t g t

8580 cPPT/CTS *LFLNCFNLIKPNIVPV a a c t a a a g a a t t a c a a a a a c a a a t t a c a a a a a t t c a a a a t t t t c g g g t t t a t t a c a g g g a t t g a t t t c t t a a t g t t t t t g t t t a a t g t t t t t a a g t t t t a a a a g c c c a a a t a a t g t c c c t

8640 hPGK promoter ASIWKPRPELPNPNRRKWPL c a g c a g a g a t c c a c t t t g g c c g c g g c t c g a g g g g g t t g g g g t t g c g c c t t t t c c a a g g c a g t c g t c t c t a g g t g a a a c c g g c g c c g a g c t c c c c c a a c c c c a a c g c g g a a a a g g t t c c g t

8700 hPGK promoter GPNACPRPQEPRPEPFRLPA g c c c t g g g t t t g c g c a g g g a c g c g g c t g c t c t g g g c g t g g t t c c g g g a a a c g c a g c g g c g c g g g a c c c a a a c g c g t c c c t g c g c c g a c g a g a c c c g c a c c a a g g c c c t t t g c g t c g c c g c

8760 hPGK promoter SGPDRVNKVDTRLTVRIKAA c c g a c c c t g g g t c t c g c a c a t t c t t c a c g t c c g t t c g c a g c g t c a c c c g g a t c t t c g c c g g g c t g g g a c c c a g a g c g t g t a a g a a g t g c a g g c a a g c g t c g c a g t g g g c c t a g a a g c g g c

8820 hPGK promoter VRTPGGAVSGAGGRLRSPEK c t a c c c t t g t g g g c c c c c c g g c g a c g c t t c c t g c t c c g c c c c t a a g t c g g g a a g g t t c c t g a t g g g a a c a c c c g g g g g g c c g c t g c g a a g g a c g a g g c g g g g a t t c a g c c c t t c c a a g g a

8880 hPGK promoter RNAAHRVHCVSAARRVLVRA t g c g g t t c g c g g c g t g c c g g a c g t g a c a a a c g g a a g c c g c a c g t c t c a c t a g t a c c c t c g a c g c c a a g c g c c g c a c g g c c t g c a c t g t t t g c c t t c g g c g t g c a g a g t g a t c a t g g g a g c

8940 hPGK promoter MAARRPRWAVAN SPCRWPAIAARRGRHATALL c a g a c g g a c a g c g c c a g g g a g c a a t g g c a g c g c g c c g a c c g c g a t g g g c t g t g g c c a a t a g t c t g c c t g t c g c g g t c c c t c g t t a c c g t c g c g c g g c t g g c g c t a c c c g a c a c c g g t t a t

AscI 9000 hPGK promoter SGCSAGRAESSGREGAVREA PQEAPRASLLPRSPATRSAP *CPAGLAAAPFPRHPLR g c g g c t g c t c a g c a g g g c g c g c c g a g a g c a g c g g c c g g g a a g g g g c g g t g c g g g a g g c g g c g c c g a c g a g t c g t c c c g c g c g g c t c t c g t c g c c g g c c c t t c c c c g c c a c g c c c t c c g c c

9060 hPGK promoter G T G T T C C G C A T T C T G C A A G hPGK-F GCGAVVWALFLPARCSAFCK HPATTHARNRGARHEANQLG PTPRYHPGQEQGRPTGCEAL g g t g t g g g g c g g t a g t g t g g g c c c t g t t c c t g c c c g c g c g g t g t t c c g c a t t c t g c a a g c c c a c a c c c c g c c a t c a c a c c c g g g a c a a g g a c g g g c g c g c c a c a a g g c g t a a g a c g t t c g

9120 hPGK promoter PPERTSAVGSLVDRITDLSP GSRVDATPERTSRIVSREGW RRLARRCDAGENVSDGVERG c t c c g g a g c g c a c g t c g g c a g t c g g c t c c c t c g t t g a c c g a a t c a c c g a c c t c t c t c c c c g a g g c c t c g c g t g c a g c c g t c a g c c g a g g g a g c a a c t g g c t t a g t g g c t g g a g a g a g g g g

9180 1 5 10 MTEYKPTVRLAT hPGK promoter PuroR T A C T G G C T C A T G T T C G G G T G Puro-R QGDPPELTMTEYKPTVRLAT PSGGSSVM LPIWRLKGHGLVLGRHAEGG a g g g g g a t c c a c c g g a g c t t a c c a t g a c c g a g t a c a a g c c c a c g g t g c g c c t c g c c a c c c t c c c c c t a g g t g g c c t c g a a t g g t a c t g g c t c a t g t t c g g g t g c c a c g c g g a g c g g t g g g

BsiWI 9240 15 20 25 30 RDDVPRAVRTLAAAFADYPA PuroR RDDVPRAVRTLAAAFADYPA AVVDGPGYAGEGGREGVVGG g c g a c g a c g t c c c c a g g g c c g t a c g c a c c c t c g c c g c c g c g t t c g c c g a c t a c c c c g c c a c g c t g c t g c a g g g g t c c c g g c a t g c g t g g g a g c g g c g g c g c a a g c g g c t g a t g g g g c g g t

RsrII 9300 35 40 45 50 TRHTVDPDRHIERVTELQEL PuroR TRHTVDPDRHIERVTELQEL RAVGDIRVAVDLPDGLQLFE c g c g c c a c a c c g t c g a t c c g g a c c g c c a c a t c g a g c g g g t c a c c g a g c t g c a a g a a c t c t g c g c g g t g t g g c a g c t a g g c c t g g c g g t g t a g c t c g c c c a g t g g c t c g a c g t t c t t g a g a

9360 55 60 65 70 FLTRVGLDIGKVWVADDGAA PuroR FLTRVGLDIGKVWVADDGAA EERADPEVDALHPDRVVAGR t c c t c a c g c g c g t c g g g c t c g a c a t c g g c a a g g t g t g g g t c g c g g a c g a c g g c g c c g c g g a g g a g t g c g c g c a g c c c g a g c t g t a g c c g t t c c a c a c c c a g c g c c t g c t g c c g c g g c g c c

9420 75 80 85 90 VAVWTTPESVEAGAVFAEIG PuroR VAVWTTPESVEAGAVFAEIG HRDPGRRLADFRPRHEGLDA t g g c g g t c t g g a c c a c g c c g g a g a g c g t c g a a g c g g g g g c g g t g t t c g c c g a g a t c g g c c a c c g c c a g a c c t g g t g c g g c c t c t c g c a g c t t c g c c c c c g c c a c a a g c g g c t c t a g c c g g

9480 95 100 105 110 PRMAELSGSRLAAQQQMEGL PuroR PRMAELSGSRLAAQQQMEGL RAHGLQATGPQGRLLLHFAE c g c g c a t g g c c g a g t t g a g c g g t t c c c g g c t g g c c g c g c a g c a a c a g a t g g a a g g c c t c c g c g c g t a c c g g c t c a a c t c g c c a a g g g c c g a c c g g c g c g t c g t t g t c t a c c t t c c g g a g g

9540 115 120 125 130 LAPHRPKEPAWFLATVGVSP PuroR LAPHRPKEPAWFLATVGVSP QRRVPGLLGRPEQGGDADRG t g g c g c c g c a c c g g c c c a a g g a g c c c g c g t g g t t c c t g g c c a c c g t c g g c g t c t c g c c c g a c c g c g g c g t g g c c g g g t t c c t c g g g c g c a c c a a g g a c c g g t g g c a g c c g c a g a g c g g g c

9600 135 140 145 150 DHQGKGLGSAVVLPGVEAAE PuroR DHQGKGLGSAVVLPGVEAAE VVLALTQAAGDHEGSHLRGL a c c a c c a g g g c a a g g g t c t g g g c a g c g c c g t c g t g c t c c c c g g a g t g g a g g c g g c c g a g c t g g t g g t c c c g t t c c c a g a c c c g t c g c g g c a g c a c g a g g g g c c t c a c c t c c g c c g g c t c g

9660 155 160 165 170 RAGVPAFLETSAPRNLPFYE PuroR G C A A C C T C C C C T T C T A C G A G C Puro-F RAGVPAFLETSAPRNLPFYE AGPHGGEQLGGRGAVEGEVL g c g c c g g g g t g c c c g c c t t c c t g g a g a c c t c c g c g c c c c g c a a c c t c c c c t t c t a c g a g c c g c g g c c c c a c g g g c g g a a g g a c c t c t g g a g g c g c g g g g c g t t g g a g g g g a a g a t g c t c g

9720 175 180 185 190 RLGFTVTADVEVPEGPRTWC PuroR RLGFTVTADVEVPEGPRTWC PEAEGDGGVDLHGFSRAGPA g g c t c g g c t t c a c c g t c a c c g c c g a c g t c g a g g t g c c c g a a g g a c c g c g c a c c t g g t g c a c c g a g c c g a a g t g g c a g t g g c g g c t g c a g c t c c a c g g g c t t c c t g g c g c g t g g a c c a c g t

9780 195 200 MTRKPGA* PuroR MTRKPGA* HGALGTGSARGVVRLARGFP t g a c c c g c a a g c c c g g t g c c t g a c g c c c g c c c c a c g a c c c g c a g c g c c c g a c c g a a a g g a a c t g g g c g t t c g g g c c a c g g a c t g c g g g c g g g g t g c t g g g c g t c g c g g g c t g g c t t t c c t

Acc65IKpnI 9840 ACSGM g c g c a c g a c c c c a t g c a t c g g t a c c t t t a a g a c c a a t g a c t t a c a a g g c a g c t g t a g a t c c g c g t g c t g g g g t a c g t a g c c a t g g a a a t t c t g g t t a c t g a a t g t t c c g t c g a c a t c t a g

9900 3' LTR ( Δ U3) t t a g c c a c t t t t t a a a a g a a a a g g g g g g a c t g g a a g g g c t a a t t c a c t c c c a a c g a a g a c a a t c g g t g a a a a a t t t t c t t t t c c c c c c t g a c c t t c c c g a t t a a g t g a g g g t t g c t t c t g

9960 3' LTR ( Δ U3) a a g a t c t g c t t t t t g c t t g t a c t g g g t c t c t c t g g t t a g a c c a g a t c t g a g c c t g g g a g c t t c t a g a c g a a a a a c g a a c a t g a c c c a g a g a g a c c a a t c t g g t c t a g a c t c g g a c c c t c g

10,020 3' LTR ( Δ U3) t c t c t g g c t a a c t a g g g a a c c c a c t g c t t a a g c c t c a a t a a a g c t t g c c t t g a g t g c t t c a g a g a c c g a t t g a t c c c t t g g g t g a c g a a t t c g g a g t t a t t t c g a a c g g a a c t c a c g a a g

10,080 3' LTR ( Δ U3) a a g t a g t g t g t g c c c g t c t g t t g t g t g a c t c t g g t a a c t a g a g a t c c c t c a g a c c c t t t t t t c a t c a c a c a c g g g c a g a c a a c a c a c t g a g a c c a t t g a t c t c t a g g g a g t c t g g g a a a a

10,140 3' LTR ( Δ U3) a g t c a g t g t g g a a a a t c t c t a g c a g t a g t a g t t c a t g t c a t c t t a t t a t t c a g t a t t t a t t c a g t c a c a c c t t t t a g a g a t c g t c a t c a t c a a g t a c a g t a g a a t a a t a a g t c a t a a a t a

10,200 SV40 poly(A) signal a a c t t g c a a a g a a a t g a a t a t c a g a g a g t g a g a g g a a c t t g t t t a t t g c a g c t t a t a a t g t t g a a c g t t t c t t t a c t t a t a g t c t c t c a c t c t c c t t g a a c a a a t a a c g t c g a a t a t t a c

10,260 SV40 poly(A) signal C G T T A T C G T A G T G T T T A A A G SV40pA-R g t t a c a a a t a a a g c a a t a g c a t c a c a a a t t t c a c a a a t a a a g c a t t t t t t t c a c t g c a t t c a a t g t t t a t t t c g t t a t c g t a g t g t t t a a a g t g t t t a t t t c g t a a a a a a a g t g a c g t a a

10,320 SV40 poly(A) signal G T G G T T T G T C C A A A C T C A T C EBV-rev c t a g t t g t g g t t t g t c c a a a c t c a t c a a t g t a t c t t a t c a t g t c t g g c t c t a g c t a t c c c g a t c a a c a c c a a a c a g g t t t g a g t a g t t a c a t a g a a t a g t a c a g a c c g a g a t c g a t a g g g

10,380 SV40 ori g c c c c t a a c t c c g c c c a t c c c g c c c c t a a c t c c g c c c a g t t c c g c c c a t t c t c c g c c c c a c g g g g a t t g a g g c g g g t a g g g c g g g g a t t g a g g c g g g t c a a g g c g g g t a a g a g g c g g g g t

SfiI 10,440 SV40 ori t g g c t g a c t a a t t t t t t t t a t t t a t g c a g a g g c c g a g g c c g c c t c g g c c t c t g a g c t a t t a c c g a c t g a t t a a a a a a a a t a a a t a c g t c t c c g g c t c c g g c g g a g c c g g a g a c t c g a t a a

10,500 SV40 ori T7 promoter G G G A T A T C A T7 c c a g a a g t a g t g a g g a g g c t t t t t t g g a g g c c t a g g g a c g t a c c c a a t t c g c c c t a t a g t g g t c t t c a t c a c t c c t c c g a a a a a a c c t c c g g a t c c c t g c a t g g g t t a a g c g g g a t a t c a

10,560 T7 promoter M13 fwd C T C A G C A T A A T T7 T G A C C G G C A G C A A A A T G T M13 Forward G C A A A A T G T T G C A G C A C T G A C C C M13/pUC Forward g a g t c g t a t t a c g c g c g c t c a c t g g c c g t c g t t t t a c a a c g t c g t g a c t g g g a a a a c c c t c t c a g c a t a a t g c g c g c g a g t g a c c g g c a g c a a a a t g t t g c a g c a c t g a c c c t t t t g g g a

10,620 g g c g t t a c c c a a c t t a a t c g c c t t g c a g c a c a t c c c c c t t t c g c c a g c t g g c g t a a t a g c c c g c a a t g g g t t g a a t t a g c g g a a c g t c g t g t a g g g g g a a a g c g g t c g a c c g c a t t a t c g

10,680 f1 ori g a a g a g g c c c g c a c c g a t c g c c c t t c c c a a c a g t t g c g c a g c c t g a a t g g c g a a t g g g a c c t t c t c c g g g c g t g g c t a g c g g g a a g g g t t g t c a a c g c g t c g g a c t t a c c g c t t a c c c t g

10,740 f1 ori g c g c c c t g t a g c g g c g c a t t a a g c g c g g c g g g t g t g g t g g t t a c g c g c a g c g t g a c c g c t c g c g g g a c a t c g c c g c g t a a t t c g c g c c g c c c a c a c c a c c a a t g c g c g t c g c a c t g g c g a

10,800 f1 ori G A G G A A A G C G A A A G A A G G G A F1ori-R a c a c t t g c c a g c g c c c t a g c g c c c g c t c c t t t c g c t t t c t t c c c t t c c t t t c t c g c c a c g t g t g a a c g g t c g c g g g a t c g c g g g c g a g g a a a g c g a a a g a a g g g a a g g a a a g a g c g g t g c

10,860 f1 ori t t c g c c g g c t t t c c c c g t c a a g c t c t a a a t c g g g g g c t c c c t t t a g g g t t c c g a t t t a g t a a g c g g c c g a a a g g g g c a g t t c g a g a t t t a g c c c c c g a g g g a a a t c c c a a g g c t a a a t c a

10,920 f1 ori g c t t t a c g g c a c c t c g a c c c c a a a a a a c t t g a t t a g g g t g a t g g t t c a c g t a g t g g g c c a c g a a a t g c c g t g g a g c t g g g g t t t t t t g a a c t a a t c c c a c t a c c a a g t g c a t c a c c c g g t

10,980 f1 ori G T G G A F1ori-F t c g c c c t g a t a g a c g g t t t t t c g c c c t t t g a c g t t g g a g t c c a c g t t c t t t a a t a g t g g a a g c g g g a c t a t c t g c c a a a a a g c g g g a a a c t g c a a c c t c a g g t g c a a g a a a t t a t c a c c t

11,040 f1 ori C T C T T G T T C C A A A C T G G F1ori-F c t c t t g t t c c a a a c t g g a a c a a c a c t c a a c c c t a t c t c g g t c t a t t c t t t t g a t t t a t a a g a g a a c a a g g t t t g a c c t t g t t g t g a g t t g g g a t a g a g c c a g a t a a g a a a a c t a a a t a t t

11,100 f1 ori g g g a t t t t g c c g a t t t c g g c c t a t t g g t t a a a a a a t g a g c t g a t t t a a c a a a a a t t t a a c c c c t a a a a c g g c t a a a g c c g g a t a a c c a a t t t t t t a c t c g a c t a a a t t g t t t t t a a a t t g

11,160 f1 ori AmpR promoter g c g a a t t t t a a c a a a a t a t t a a c g c t t a c a a t t t a g g t g g c a c t t t t c g g g g a a a t g t g c c g c t t a a a a t t g t t t t a t a a t t g c g a a t g t t a a a t c c a c c g t g a a a a g c c c c t t t a c a c g

11,220 AmpR promoter g c g g a a c c c c t a t t t g t t t a t t t t t c t a a a t a c a t t c a a a t a t g t a t c c g c t c a t g a g a c c g c c t t g g g g a t a a a c a a a t a a a a a g a t t t a t g t a a g t t t a t a c a t a g g c g a g t a c t c t g

11,280 1 5 signal sequence MSIQH AmpR promoter AmpR MSIQH a a t a a c c c t g a t a a a t g c t t c a a t a a t a t t g a a a a a g g a a g a g t a t g a g t a t t c a a c a t t t t a t t g g g a c t a t t t a c g a a g t t a t t a t a a c t t t t t c c t t c t c a t a c t c a t a a g t t g t a a

11,340 10 15 20 signal sequence FRVALIPFFAAFCLPVFA 25 HP AmpR FRVALIPFFAAFCLPVFAHP t c c g t g t c g c c c t t a t t c c c t t t t t t g c g g c a t t t t g c c t t c c t g t t t t t g c t c a c c c a g a g g c a c a g c g g g a a t a a g g g a a a a a a c g c c g t a a a a c g g a a g g a c a a a a a c g a g t g g g t c

11,400 30 35 40 45 ETLVKVKDAEDQLGARVGYI AmpR ETLVKVKDAEDQLGARVGYI a a a c g c t g g t g a a a g t a a a a g a t g c t g a a g a t c a g t t g g g t g c a c g a g t g g g t t a c a t c g t t t g c g a c c a c t t t c a t t t t c t a c g a c t t c t a g t c a a c c c a c g t g c t c a c c c a a t g t a g c

11,460 50 55 60 65 ELDLNSGKILESFRPEERFP AmpR ELDLNSGKILESFRPEERFP a a c t g g a t c t c a a c a g c g g t a a g a t c c t t g a g a g t t t t c g c c c c g a a g a a c g t t t t c c a a t t g a c c t a g a g t t g t c g c c a t t c t a g g a a c t c t c a a a a g c g g g g c t t c t t g c a a a a g g t t

11,520 70 75 80 85 MMSTFKVLLCGAVLSRIDAG AmpR C G A T A C A C C G C G C C A T A A T A Amp-R MMSTFKVLLCGAVLSRIDAG t g a t g a g c a c t t t t a a a g t t c t g c t a t g t g g c g c g g t a t t a t c c c g t a t t g a c g c c g g g c a c t a c t c g t g a a a a t t t c a a g a c g a t a c a c c g c g c c a t a a t a g g g c a t a a c t g c g g c c c g

11,580 90 95 100 105 QEQLGRRIHYSQNDLVEYSP AmpR QEQLGRRIHYSQNDLVEYSP a a g a g c a a c t c g g t c g c c g c a t a c a c t a t t c t c a g a a t g a c t t g g t t g a g t a c t c a c c a g t t c t c g t t g a g c c a g c g g c g t a t g t g a t a a g a g t c t t a c t g a a c c a a c t c a t g a g t g g t c

11,640 110 115 120 125 VTEKHLTDGMTVRELCSAAI AmpR VTEKHLTDGMTVRELCSAAI t c a c a g a a a a g c a t c t t a c g g a t g g c a t g a c a g t a a g a g a a t t a t g c a g t g c t g c c a t a a a g t g t c t t t t c g t a g a a t g c c t a c c g t a c t g t c a t t c t c t t a a t a c g t c a c g a c g g t a t t

11,700 130 135 140 145 TMSDNTAANLLLTTIGGPKE AmpR TMSDNTAANLLLTTIGGPKE c c a t g a g t g a t a a c a c t g c g g c c a a c t t a c t t c t g a c a a c g a t c g g a g g a c c g a a g g a g c g g t a c t c a c t a t t g t g a c g c c g g t t g a a t g a a g a c t g t t g c t a g c c t c c t g g c t t c c t c g

11,760 150 155 160 165 LTAFLHNMGDHVTRLDRWEP AmpR LTAFLHNMGDHVTRLDRWEP t a a c c g c t t t t t t g c a c a a c a t g g g g g a t c a t g t a a c t c g c c t t g a t c g t t g g g a a c c g g a t t g g c g a a a a a a c g t g t t g t a c c c c c t a g t a c a t t g a g c g g a a c t a g c a a c c c t t g g c c

11,820 170 175 180 185 ELNEAIPNDERDTTMPVAMA AmpR ELNEAIPNDERDTTMPVAMA a g c t g a a t g a a g c c a t a c c a a a c g a c g a g c g t g a c a c c a c g a t g c c t g t a g c a a t g g c a a t c g a c t t a c t t c g g t a t g g t t t g c t g c t c g c a c t g t g g t g c t a c g g a c a t c g t t a c c g t t

11,880 190 195 200 205 TTLRKLLTGELLTLASRQQL AmpR TTLRKLLTGELLTLASRQQL c a a c g t t g c g c a a a c t a t t a a c t g g c g a a c t a c t t a c t c t a g c t t c c c g g c a a c a a t t a a g t t g c a a c g c g t t t g a t a a t t g a c c g c t t g a t g a a t g a g a t c g a a g g g c c g t t g t t a a t t

11,940 210 215 220 225 IDWMEADKVAGPLLRSALPA AmpR IDWMEADKVAGPLLRSALPA t a g a c t g g a t g g a g g c g g a t a a a g t t g c a g g a c c a c t t c t g c g c t c g g c c c t t c c g g c t g a t c t g a c c t a c c t c c g c c t a t t t c a a c g t c c t g g t g a a g a c g c g a g c c g g g a a g g c c g a c

12,000 230 235 240 245 GWFIADKSGAGERGSRGIIA AmpR GWFIADKSGAGERGSRGIIA g c t g g t t t a t t g c t g a t a a a t c t g g a g c c g g t g a g c g t g g g t c t c g c g g t a t c a t t g c a g c g a c c a a a t a a c g a c t a t t t a g a c c t c g g c c a c t c g c a c c c a g a g c g c c a t a g t a a c g t c

12,060 250 255 260 265 ALGPDGKPSRIVVIYTTGSQ AmpR ALGPDGKPSRIVVIYTTGSQ c a c t g g g g c c a g a t g g t a a g c c c t c c c g t a t c g t a g t t a t c t a c a c g a c g g g g a g t c a g g g t g a c c c c g g t c t a c c a t t c g g g a g g g c a t a g c a t c a a t a g a t g t g c t g c c c c t c a g t c c

12,120 270 275 280 285 ATMDERNRQIAEIGASLIKH AmpR ATMDERNRQIAEIGASLIKH c a a c t a t g g a t g a a c g a a a t a g a c a g a t c g c t g a g a t a g g t g c c t c a c t g a t t a a g c a t t g t t g a t a c c t a c t t g c t t t a t c t g t c t a g c g a c t c t a t c c a c g g a g t g a c t a a t t c g t a a

12,180 W* AmpR W* g g t a a c t g t c a g a c c a a g t t t a c t c a t a t a t a c t t t a g a t t g a t t t a a a a c t t c a t t t t t c c a t t g a c a g t c t g g t t c a a a t g a g t a t a t a t g a a a t c t a a c t a a a t t t t g a a g t a a a a a

12,240 a a t t t a a a a g g a t c t a g g t g a a g a t c c t t t t t g a t a a t c t c a t g a c c a a a a t c c c t t a a c t t a a a t t t t c c t a g a t c c a c t t c t a g g a a a a a c t a t t a g a g t a c t g g t t t t a g g g a a t t g

12,300 ori g t g a g t t t t c g t t c c a c t g a g c g t c a g a c c c c g t a g a a a a g a t c a a a g g a t c t t c t t g a g c a c t c a a a a g c a a g g t g a c t c g c a g t c t g g g g c a t c t t t t c t a g t t t c c t a g a a g a a c t c

12,360 ori a t c c t t t t t t t c t g c g c g t a a t c t g c t g c t t g c a a a c a a a a a a a c c a c c g c t a c c a g c g g t a g g a a a a a a a g a c g c g c a t t a g a c g a c g a a c g t t t g t t t t t t t g g t g g c g a t g g t c g c c

12,420 ori t g g t t t g t t t g c c g g a t c a a g a g c t a c c a a c t c t t t t t c c g a a g g t a a c t g g c t t c a g c a a c c a a a c a a a c g g c c t a g t t c t c g a t g g t t g a g a a a a a g g c t t c c a t t g a c c g a a g t c g t

12,480 ori g a g c g c a g a t a c c a a a t a c t g t t c t t c t a g t g t a g c c g t a g t t a g g c c a c c a c t t c a a g a c t c g c g t c t a t g g t t t a t g a c a a g a a g a t c a c a t c g g c a t c a a t c c g g t g g t g a a g t t c t

12,540 ori a c t c t g t a g c a c c g c c t a c a t a c c t c g c t c t g c t a a t c c t g t t a c c a g t g g c t g c t g c c a t g a g a c a t c g t g g c g g a t g t a t g g a g c g a g a c g a t t a g g a c a a t g g t c a c c g a c g a c g g t

12,600 ori g t g g c g a t a a g t c g t g t c t t a c c g g g t t g g a c t c a a g a c g a t a g t t a c c g g a t a a g g c g c c a c c g c t a t t c a g c a c a g a a t g g c c c a a c c t g a g t t c t g c t a t c a a t g g c c t a t t c c g c g

12,660 ori a g c g g t c g g g c t g a a c g g g g g g t t c g t g c a c a c a g c c c a g c t t g g a g c g a a c g a c c t a c a t c g c c a g c c c g a c t t g c c c c c c a a g c a c g t g t g t c g g g t c g a a c c t c g c t t g c t g g a t g t

12,720 ori c c g a a c t g a g a t a c c t a c a g c g t g a g c t a t g a g a a a g c g c c a c g c t t c c c g a a g g g a g a a g g c t t g a c t c t a t g g a t g t c g c a c t c g a t a c t c t t t c g c g g t g c g a a g g g c t t c c c t c t t

12,780 ori a g g c g g a c a g g t a t c c g g t a a g c g g c a g g g t c g g a a c a g g a g a g c g c a c g a g g g a g c t t c t c c g c c t g t c c a t a g g c c a t t c g c c g t c c c a g c c t t g t c c t c t c g c g t g c t c c c t c g a a g

12,840 ori G G G A A A C G C C T G G T A T C T T T pBR322ori-F c a g g g g g a a a c g c c t g g t a t c t t t a t a g t c c t g t c g g g t t t c g c c a c c t c t g a c t t g a g c g t c c c c c t t t g c g g a c c a t a g a a a t a t c a g g a c a g c c c a a a g c g g t g g a g a c t g a a c t c g

3ʹ 5ʹ 12,884 ori g t c g a t t t t t g t g a t g c t c g t c a g g g g g g c g g a g c c t a t g g a a a c a g c t a a a a a c a c t a c g a g c a g t c c c c c c g c c t c g g a t a c c t t t

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.