Addgene PS-CFP2-Vinculin-N-21 Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: PS-CFP2-Vinculin-N-21 Sequencing Result


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pBR322ori-F (7763 .. 7782) TK-pA-R (6854 .. 6873) Neo-F (6536 .. 6555) RsrII (6516) PflFI - Tth111I (6118) FspI (6102) Neo-R (5926 .. 5945) pBABE 3' (5503 .. 5523) F1ori-R (5241 .. 5260) F1ori-F (5029 .. 5050) AflII (4882) EBV-rev (4855 .. 4874) SV40pA-R (4801 .. 4820) HpaI (4763) MfeI (4750) NotI (4644) BssHII (4248) SmaI (3904) ApaI (3903) AflIII - PciI (0) NdeI (292) SnaBI (398) CMV-F (577 .. 597) NheI (649) BmtI (653) PvuI (689) BbvCI (1049) Eco53kI (1206) SacI (1208) BclI * (1659) SgrAI (1701) BlpI (1721) EcoRV (3127) EcoRI (3875) SalI (3885) AccI (3886) SacII (3898) PspOMI (3899) TspMI - XmaI (3902) PS-CFP2-Vinculin-N-21 7918 bp

Sequence View

AflIIIPciI 5ʹ 3ʹ 60 c a t g t t c t t t c c t g c g t t a t c c c c t g a t t c t g t g g a t a a c c g t a t t a c c g c c a t g c a t t a g t a c a a g a a a g g a c g c a a t a g g g g a c t a a g a c a c c t a t t g g c a t a a t g g c g g t a c g t a a t

120 CMV enhancer g t t a t t a a t a g t a a t c a a t t a c g g g g t c a t t a g t t c a t a g c c c a t a t a t g g a g t t c c g c g c a a t a a t t a t c a t t a g t t a a t g c c c c a g t a a t c a a g t a t c g g g t a t a t a c c t c a a g g c g c

180 CMV enhancer t t a c a t a a c t t a c g g t a a a t g g c c c g c c t g g c t g a c c g c c c a a c g a c c c c c g c c c a t t g a a a t g t a t t g a a t g c c a t t t a c c g g g c g g a c c g a c t g g c g g g t t g c t g g g g g c g g g t a a c t

240 CMV enhancer c g t c a a t a a t g a c g t a t g t t c c c a t a g t a a c g c c a a t a g g g a c t t t c c a t t g a c g t c a a t g c a g t t a t t a c t g c a t a c a a g g g t a t c a t t g c g g t t a t c c c t g a a a g g t a a c t g c a g t t a

NdeI 300 CMV enhancer g g g t g g a g t a t t t a c g g t a a a c t g c c c a c t t g g c a g t a c a t c a a g t g t a t c a t a t g c c a a c c c a c c t c a t a a a t g c c a t t t g a c g g g t g a a c c g t c a t g t a g t t c a c a t a g t a t a c g g t t

360 CMV enhancer g t a c g c c c c c t a t t g a c g t c a a t g a c g g t a a a t g g c c c g c c t g g c a t t a t g c c c a g t a c a c a t g c g g g g g a t a a c t g c a g t t a c t g c c a t t t a c c g g g c g g a c c g t a a t a c g g g t c a t g t

SnaBI 420 CMV enhancer t g a c c t t a t g g g a c t t t c c t a c t t g g c a g t a c a t c t a c g t a t t a g t c a t c g c t a t t a c c a a c t g g a a t a c c c t g a a a g g a t g a a c c g t c a t g t a g a t g c a t a a t c a g t a g c g a t a a t g g t

480 CMV enhancer CMV promoter t g g t g a t g c g g t t t t g g c a g t a c a t c a a t g g g c g t g g a t a g c g g t t t g a c t c a c g g g g a t a c c a c t a c g c c a a a a c c g t c a t g t a g t t a c c c g c a c c t a t c g c c a a a c t g a g t g c c c c t a

540 CMV promoter t t c c a a g t c t c c a c c c c a t t g a c g t c a a t g g g a g t t t g t t t t g g c a c c a a a a t c a a c g g g a a g g t t c a g a g g t g g g g t a a c t g c a g t t a c c c t c a a a c a a a a c c g t g g t t t t a g t t g c c c

600 CMV promoter C G C A A A T G G G C G G T A G G C G T G CMV-F a c t t t c c a a a a t g t c g t a a c a a c t c c g c c c c a t t g a c g c a a a t g g g c g g t a g g c g t g t a c t g a a a g g t t t t a c a g c a t t g t t g a g g c g g g g t a a c t g c g t t t a c c c g c c a t c c g c a c a t g

BmtINheI 660 CMV promoter g g t g g g a g g t c t a t a t a a g c a g a g c t g g t t t a g t g a a c c g t c a g a t c c g c t a g c a c c g c c c c a c c c t c c a g a t a t a t t c g t c t c g a c c a a a t c a c t t g g c a g t c t a g g c g a t c g t g g c g g

PvuI 720 MPVFHTRTIESILEPVAQQ a c c a t g c c a g t g t t t c a t a c g c g c a c g a t c g a g a g c a t c c t g g a g c c g g t g g c a c a g c a g t g g t a c g g t c a c a a a g t a t g c g c g t g c t a g c t c t c g t a g g a c c t c g g c c a c c g t g t c g t c

780 ISHLVIMHEEGEVDGKAIPD a t c t c c c a c c t g g t g a t a a t g c a c g a g g a g g g c g a g g t g g a c g g c a a a g c c a t t c c t g a c t a g a g g g t g g a c c a c t a t t a c g t g c t c c t c c c g c t c c a c c t g c c g t t t c g g t a a g g a c t g

840 LTAPVAAVQAAVSNLVRVGK c t c a c c g c g c c c g t g g c c g c c g t g c a g g c g g c c g t c a g c a a c c t c g t c c g g g t t g g a a a a g a g t g g c g c g g g c a c c g g c g g c a c g t c c g c c g g c a g t c g t t g g a g c a g g c c c a a c c t t t t

900 ETVQTTEDQILKRDMPPAFI g a g a c t g t t c a a a c c a c t g a g g a t c a g a t t t t g a a g a g a g a t a t g c c a c c a g c a t t t a t t c t c t g a c a a g t t t g g t g a c t c c t a g t c t a a a a c t t c t c t c t a t a c g g t g g t c g t a a a t a a

960 KVENACTKLVQAAQMLQSDP a a g g t t g a g a a t g c t t g c a c c a a g c t t g t c c a g g c a g c t c a g a t g c t t c a g t c a g a c c c t t t c c a a c t c t t a c g a a c g t g g t t c g a a c a g g t c c g t c g a g t c t a c g a a g t c a g t c t g g g a

1020 YSVPARDYLIDGSRGILSGT t a c t c a g t g c c t g c t c g a g a t t a t c t a a t t g a t g g g t c a a g g g g c a t c c t c t c t g g a a c a a t g a g t c a c g g a c g a g c t c t a a t a g a t t a a c t a c c c a g t t c c c c g t a g g a g a g a c c t t g t

BbvCI 1080 SDLLLTFDEAEVRKIIRVCK t c a g a c c t g c t c c t t a c c t t c g a t g a g g c t g a g g t c c g t a a a a t t a t t a g a g t t t g c a a a a g t c t g g a c g a g g a a t g g a a g c t a c t c c g a c t c c a g g c a t t t t a a t a a t c t c a a a c g t t t

1140 GILEYLTVAEVVETMEDLVT g g a a t t t t g g a a t a t c t t a c a g t g g c a g a g g t g g t g g a g a c t a t g g a a g a t t t g g t c a c t c c t t a a a a c c t t a t a g a a t g t c a c c g t c t c c a c c a c c t c t g a t a c c t t c t a a a c c a g t g a

1200 YTKNLGPGMTKMAKMIDERQ t a c a c a a a g a a t c t t g g g c c a g g a a t g a c t a a g a t g g c c a a g a t g a t t g a c g a g a g a c a g a t g t g t t t c t t a g a a c c c g g t c c t t a c t g a t t c t a c c g g t t c t a c t a a c t g c t c t c t g t c

Eco53kISacI 1260 QELTHQEHRVMLVNSMNTVK c a g g a g c t c a c t c a c c a g g a g c a c c g a g t g a t g t t g g t g a a c t c g a t g a a c a c c g t g a a a g t c c t c g a g t g a g t g g t c c t c g t g g c t c a c t a c a a c c a c t t g a g c t a c t t g t g g c a c t t t

1320 ELLPVLISAMKIFVTTKNSK g a g t t g c t g c c a g t t c t c a t t t c a g c t a t g a a g a t t t t t g t a a c a a c t a a a a a c t c a a a a c t c a a c g a c g g t c a a g a g t a a a g t c g a t a c t t c t a a a a a c a t t g t t g a t t t t t g a g t t t t

1380 NQGIEEALKNRNFTLEKMSA a a c c a a g g c a t a g a g g a a g c t t t a a a a a a t c g c a a t t t t a c t c t a g a a a a a a t g a g t g c t t t g g t t c c g t a t c t c c t t c g a a a t t t t t t a g c g t t a a a a t g a g a t c t t t t t t a c t c a c g a

1440 EINEIIRVLQLTSWDEDAWA g a a a t t a a t g a g a t a a t t c g t g t g t t a c a a c t c a c c t c t t g g g a t g a a g a t g c c t g g g c c c t t t a a t t a c t c t a t t a a g c a c a c a a t g t t g a g t g g a g a a c c c t a c t t c t a c g g a c c c g g

1500 SKDTEAMKRALASIDSKLNQ a g c a a g g a c a c t g a a g c c a t g a a g a g a g c a t t g g c c t c c a t a g a c t c c a a a c t g a a c c a g t c g t t c c t g t g a c t t c g g t a c t t c t c t c g t a a c c g g a g g t a t c t g a g g t t t g a c t t g g t c

1560 AKGWLRDPSASPGDAGEQAI g c c a a a g g t t g g c t c c g t g a c c c t a g t g c c t c c c c a g g g g a t g c t g g t g a g c a g g c c a t c c g g t t t c c a a c c g a g g c a c t g g g a t c a c g g a g g g g t c c c c t a c g a c c a c t c g t c c g g t a g

1620 RQILDEAGKVGELCAGKERR a g a c a g a t c t t a g a t g a a g c t g g a a a a g t t g g t g a a c t c t g t g c a g g c a a a g a a c g c a g g t c t g t c t a g a a t c t a c t t c g a c c t t t t c a a c c a c t t g a g a c a c g t c c g t t t c t t g c g t c c

BclI* 1680 EILGTCKMLGQMTDQVADLR g a g a t t c t g g g a a c t t g c a a a a t g c t a g g g c a g a t g a c t g a t c a a g t g g c t g a c c t c c g t c t c t a a g a c c c t t g a a c g t t t t a c g a t c c c g t c t a c t g a c t a g t t c a c c g a c t g g a g g c a

BlpISgrAI 1740 ARGQGSSPVAMQKAQQVSQG g c c a g a g g a c a a g g a t c c t c a c c g g t g g c c a t g c a g a a a g c t c a g c a g g t a t c t c a g g g t c g g t c t c c t g t t c c t a g g a g t g g c c a c c g g t a c g t c t t t c g a g t c g t c c a t a g a g t c c c a

1800 LDVLTAKVENAARKLEAMTN c t g g a t g t g c t c a c a g c a a a a g t g g a a a a t g c a g c t c g c a a g c t g g a a g c c a t g a c c a a c g a c c t a c a c g a g t g t c g t t t t c a c c t t t t a c g t c g a g c g t t c g a c c t t c g g t a c t g g t t g

1860 SKQSIAKKIDAAQNWLADPN t c a a a g c a g a g c a t t g c a a a g a a g a t c g a t g c t g c t c a g a a c t g g c t t g c a g a t c c a a a t a g t t t c g t c t c g t a a c g t t t c t t c t a g c t a c g a c g a g t c t t g a c c g a a c g t c t a g g t t t a

1920 GGPEGEEQIRGALAEARKIA g g t g g a c c g g a a g g a g a a g a g c a g a t t c g a g g t g c t t t g g c t g a a g c t c g g a a a a t a g c a c c a c c t g g c c t t c c t c t t c t c g t c t a a g c t c c a c g a a a c c g a c t t c g a g c c t t t t a t c g t

1980 ELCDDPKERDDILRSLGEIS g a a t t a t g t g a t g a t c c t a a a g a a a g a g a t g a c a t t c t a c g t t c c c t t g g g g a a a t a t c t c t t a a t a c a c t a c t a g g a t t t c t t t c t c t a c t g t a a g a t g c a a g g g a a c c c c t t t a t a g a

2040 ALTSKLADLRRQGKGDSPEA g c t c t g a c t t c t a a a t t a g c a g a t c t a c g a a g a c a g g g g a a a g g a g a t t c t c c a g a g g c t c g a g a c t g a a g a t t t a a t c g t c t a g a t g c t t c t g t c c c c t t t c c t c t a a g a g g t c t c c g a

2100 RALAKQVATALQNLQTKTNR c g a g c c t t g g c c a a a c a g g t g g c c a c g g c c c t g c a g a a c c t g c a g a c c a a a a c c a a c c g g g c t c g g a a c c g g t t t g t c c a c c g g t g c c g g g a c g t c t t g g a c g t c t g g t t t t g g t t g g c c

2160 AVANSRPAKAAVHLEGKIEQ g c t g t g g c c a a c a g c a g a c c g g c c a a a g c a g c t g t a c a c c t t g a g g g c a a g a t t g a g c a a c g a c a c c g g t t g t c g t c t g g c c g g t t t c g t c g a c a t g t g g a a c t c c c g t t c t a a c t c g t t

2220 AQRWIDNPTVDDRGVGQAAI g c a c a g c g g t g g a t t g a t a a t c c c a c a g t g g a t g a c c g t g g a g t c g g t c a g g c t g c c a t c c g t g t c g c c a c c t a a c t a t t a g g g t g t c a c c t a c t g g c a c c t c a g c c a g t c c g a c g g t a g

2280 RGLVAEGHRLANVMMGPYRQ c g g g g g c t t g t g g c c g a a g g g c a t c g t c t g g c t a a t g t t a t g a t g g g g c c t t a t c g g c a a g c c c c c g a a c a c c g g c t t c c c g t a g c a g a c c g a t t a c a a t a c t a c c c c g g a a t a g c c g t t

2340 DLLAKCDRVDQLTAQLADLA g a t c t t c t c g c c a a g t g t g a c c g a g t g g a c c a g c t g a c a g c c c a g c t g g c t g a c c t g g c t c t a g a a g a g c g g t t c a c a c t g g c t c a c c t g g t c g a c t g t c g g g t c g a c c g a c t g g a c c g a

2400 ARGEGESPQARALASQLQDS g c c a g a g g g g a a g g g g a g a g t c c t c a g g c a c g a g c a c t t g c a t c t c a g c t c c a a g a c t c c c g g t c t c c c c t t c c c c t c t c a g g a g t c c g t g c t c g t g a a c g t a g a g t c g a g g t t c t g a g g

2460 LKDLKARMQEAMTQEVSDVF t t a a a g g a t c t a a a a g c t c g g a t g c a g g a g g c c a t g a c t c a g g a a g t g t c a g a t g t t t t c a a t t t c c t a g a t t t t c g a g c c t a c g t c c t c c g g t a c t g a g t c c t t c a c a g t c t a c a a a a g

2520 SDTTTPIKLLAVAATAPPDA a g c g a t a c c a c a a c t c c c a t c a a g c t g t t g g c a g t g g c a g c c a c g g c g c c t c c t g a t g c g t c g c t a t g g t g t t g a g g g t a g t t c g a c a a c c g t c a c c g t c g g t g c c g c g g a g g a c t a c g c

2580 PNREEVFDERAANFENHSGK c c t a a c a g g g a a g a g g t a t t t g a t g a g a g g g c a g c t a a c t t t g a a a a c c a t t c a g g a a a g g g a t t g t c c c t t c t c c a t a a a c t a c t c t c c c g t c g a t t g a a a c t t t t g g t a a g t c c t t t c

2640 LGATAEKAAAVGTANKSTVE c t t g g t g c t a c g g c c g a g a a g g c g g c t g c g g t t g g t a c t g c t a a t a a a t c a a c a g t g g a a g a a c c a c g a t g c c g g c t c t t c c g c c g a c g c c a a c c a t g a c g a t t a t t t a g t t g t c a c c t t

2700 GIQASVKTARELTPQVVSAA g g c a t t c a g g c c t c a g t g a a g a c g g c c c g a g a a c t c a c a c c c c a g g t g g t c t c g g c t g c t c c g t a a g t c c g g a g t c a c t t c t g c c g g g c t c t t g a g t g t g g g g t c c a c c a g a g c c g a c g a

2760 RILLRNPGNQAAYEHFETMK c g t a t c t t a c t t a g g a a c c c t g g a a a t c a a g c t g c t t a t g a a c a t t t t g a g a c c a t g a a g g c a t a g a a t g a a t c c t t g g g a c c t t t a g t t c g a c g a a t a c t t g t a a a a c t c t g g t a c t t c

2820 NQWIDNVEKMTGLVDEAIDT a a c c a g t g g a t c g a t a a t g t t g a a a a a a t g a c a g g g c t g g t g g a c g a a g c c a t t g a t a c c t t g g t c a c c t a g c t a t t a c a a c t t t t t t a c t g t c c c g a c c a c c t g c t t c g g t a a c t a t g g

2880 KSLLDASEEAIKKDLDKCKV a a a t c t c t g t t g g a t g c t t c a g a a g a a g c a a t t a a a a a a g a c c t g g a c a a g t g c a a g g t a t t t a g a g a c a a c c t a c g a a g t c t t c t t c g t t a a t t t t t t c t g g a c c t g t t c a c g t t c c a t

2940 AMANIQPQMLVAGATSIARR g c t a t g g c c a a c a t t c a g c c t c a g a t g c t g g t t g c t g g g g c a a c c a g t a t t g c t c g t c g g c g a t a c c g g t t g t a a g t c g g a g t c t a c g a c c a a c g a c c c c g t t g g t c a t a a c g a g c a g c c

3000 ANRILLVAKREVENSEDPKF g c c a a c c g g a t c c t g c t g g t g g c t a a g a g g g a g g t g g a a a a t t c c g a g g a t c c c a a g t t c c g g t t g g c c t a g g a c g a c c a c c g a t t c t c c c t c c a c c t t t t a a g g c t c c t a g g g t t c a a g

3060 REAVKAASDELSKTISPMVM c g t g a g g c t g t g a a a g c t g c c t c t g a t g a a t t g a g c a a a a c c a t c t c c c c g a t g g t g a t g g c a c t c c g a c a c t t t c g a c g g a g a c t a c t t a a c t c g t t t t g g t a g a g g g g c t a c c a c t a c

3120 DAKAVAGNISDPGLQKSFLD g a t g c a a a a g c t g t g g c t g g a a a c a t t t c c g a c c c t g g a c t g c a a a a g a g c t t c c t g g a c c t a c g t t t t c g a c a c c g a c c t t t g t a a a g g c t g g g a c c t g a c g t t t t c t c g a a g g a c c t g

EcoRV 3180 SGYRILGAVAKVREAFQPQE t c a g g a t a t c g g a t c c t g g g a g c t g t g g c c a a g g t c a g a g a a g c c t t c c a a c c t c a g g a g a g t c c t a t a g c c t a g g a c c c t c g a c a c c g g t t c c a g t c t c t t c g g a a g g t t g g a g t c c t c

3240 PDFPPPPPDLEQLRLTDELA c c t g a c t t c c c g c c g c c t c c a c c a g a c c t t g a a c a a c t c c g a c t a a c a g a t g a g c t t g c t g g a c t g a a g g g c g g c g g a g g t g g t c t g g a a c t t g t t g a g g c t g a t t g t c t a c t c g a a c g a

3300 PPKPPLPEGEVPPPRPPPPE c c t c c c a a a c c a c c t c t g c c t g a a g g t g a g g t c c c t c c a c c t a g g c c t c c a c c a c c a g a g g g a g g g t t t g g t g g a g a c g g a c t t c c a c t c c a g g g a g g t g g a t c c g g a g g t g g t g g t c t c

3360 EKDEEFPEQKAGEVINQPMM g a a a a g g a t g a a g a g t t c c c t g a g c a g a a g g c c g g g g a g g t g a t t a a c c a g c c a a t g a t g c t t t t c c t a c t t c t c a a g g g a c t c g t c t t c c g g c c c c t c c a c t a a t t g g t c g g t t a c t a c

3420 MAARQLHDEARKWSSKGNDI a t g g c t g c c a g a c a g c t c c a t g a t g a a g c t c g c a a a t g g t c c a g c a a g g g c a a t g a c a t c t a c c g a c g g t c t g t c g a g g t a c t a c t t c g a g c g t t t a c c a g g t c g t t c c c g t t a c t g t a g

3480 IAAAKRMALLMAEMSRLVRG a t t g c a g c a g c c a a g c g c a t g g c t c t g c t g a t g g c t g a g a t g t c t c g g c t g g t a a g a g g g t a a c g t c g t c g g t t c g c g t a c c g a g a c g a c t a c c g a c t c t a c a g a g c c g a c c a t t c t c c c

3540 GSGTKRALIQCAKDIAKASD g g c a g t g g t a c c a a g c g g g c a c t c a t t c a g t g t g c c a a g g a c a t c g c c a a g g c c t c a g a t c c g t c a c c a t g g t t c g c c c g t g a g t a a g t c a c a c g g t t c c t g t a g c g g t t c c g g a g t c t a

3600 EVTRLAKEVAKQCTDKRIRT g a g g t g a c t c g g t t g g c c a a g g a g g t t g c c a a g c a g t g c a c a g a t a a a c g g a t t a g a a c c c t c c a c t g a g c c a a c c g g t t c c t c c a a c g g t t c g t c a c g t g t c t a t t t g c c t a a t c t t g g

3660 NLLQVCERIPTISTQLKILS a a c c t c t t a c a g g t a t g t g a g c g a a t c c c a a c c a t a a g c a c c c a g c t c a a a a t c c t g t c c t t g g a g a a t g t c c a t a c a c t c g c t t a g g g t t g g t a t t c g t g g g t c g a g t t t t a g g a c a g g

3720 TVKATMLGRTNISDEESEQA a c a g t g a a g g c c a c c a t g c t g g g c c g g a c c a a c a t c a g t g a t g a g g a g t c t g a g c a g g c c t g t c a c t t c c g g t g g t a c g a c c c g g c c t g g t t g t a g t c a c t a c t c c t c a g a c t c g t c c g g

3780 TEMLVHNAQNLMQSVKETVR a c a g a g a t g c t g g t t c a c a a t g c c c a g a a c c t c a t g c a g t c t g t g a a g g a g a c t g t g c g g t g t c t c t a c g a c c a a g t g t t a c g g g t c t t g g a g t a c g t c a g a c a c t t c c t c t g a c a c g c c

3840 EAEAASIKIRTDAGFTLRWV g a a g c t g a a g c t g c t t c a a t c a a a a t t c g a a c a g a t g c t g g a t t t a c a c t g c g c t g g g t t c t t c g a c t t c g a c g a a g t t a g t t t t a a g c t t g t c t a c g a c c t a a a t g t g a c g c g a c c c a a

SacIIPspOMISalIAccIEcoRI 3900 RKTPWYQSGGSGILQSTVPR a g a a a g a c t c c c t g g t a c c a g t c c g g t g g c a g c g g a a t t c t g c a g t c g a c g g t a c c g c g g t c t t t c t g a g g g a c c a t g g t c a g g c c a c c g t c g c c t t a a g a c g t c a g c t g c c a t g g c g c c

TspMIXmaIApaISmaI 3960 1 5 10 MSKGAELFTGIV PS-CFP2 ARDPPVATMSKGAELFTGIV g c c c g g g a t c c a c c g g t c g c c a c c a t g a g c a a g g g c g c c g a g c t g t t c a c c g g c a t c g t g c g g g c c c t a g g t g g c c a g c g g t g g t a c t c g t t c c c g c g g c t c g a c a a g t g g c c g t a g c a c

4020 15 20 25 30 PILIELNGDVNGHKFSVSGE PS-CFP2 PILIELNGDVNGHKFSVSGE c c c a t c c t g a t c g a g c t g a a t g g c g a t g t g a a t g g c c a c a a g t t c a g c g t g a g c g g c g a g g g g t a g g a c t a g c t c g a c t t a c c g c t a c a c t t a c c g g t g t t c a a g t c g c a c t c g c c g c t c

4080 35 40 45 50 GEGDATYGKLTLKFICTTGK PS-CFP2 GEGDATYGKLTLKFICTTGK g g c g a g g g c g a t g c c a c c t a c g g c a a g c t g a c c c t g a a g t t c a t c t g c a c c a c c g g c a a g c c g c t c c c g c t a c g g t g g a t g c c g t t c g a c t g g g a c t t c a a g t a g a c g t g g t g g c c g t t c

4140 55 60 65 70 LPVPWPTLVATLSYGVQCFS PS-CFP2 LPVPWPTLVATLSYGVQCFS c t g c c t g t g c c c t g g c c c a c c c t g g t g g c c a c c c t g a g c t a c g g c g t g c a g t g c t t c t c a g a c g g a c a c g g g a c c g g g t g g g a c c a c c g g t g g g a c t c g a t g c c g c a c g t c a c g a a g a g t

4200 75 80 85 90 RYPDHMKQHDFFKSAMPEGY PS-CFP2 RYPDHMKQHDFFKSAMPEGY c g c t a c c c c g a t c a c a t g a a g c a g c a c g a c t t c t t c a a g a g c g c c a t g c c t g a g g g c t a c g c g a t g g g g c t a g t g t a c t t c g t c g t g c t g a a g a a g t t c t c g c g g t a c g g a c t c c c g a t g

BssHII 4260 95 100 105 110 IQERTIFFEDDGNYKTRAEV PS-CFP2 IQERTIFFEDDGNYKTRAEV a t c c a g g a g c g c a c c a t c t t c t t c g a g g a t g a c g g c a a c t a c a a g a c g c g c g c c g a g g t g t a g g t c c t c g c g t g g t a g a a g a a g c t c c t a c t g c c g t t g a t g t t c t g c g c g c g g c t c c a c

4320 115 120 125 130 KFEGDTLVSRIELTGTDFKE PS-CFP2 KFEGDTLVSRIELTGTDFKE a a g t t c g a g g g c g a t a c c c t g g t g a g t c g c a t c g a g c t g a c c g g c a c t g a t t t c a a g g a g t t c a a g c t c c c g c t a t g g g a c c a c t c a g c g t a g c t c g a c t g g c c g t g a c t a a a g t t c c t c

4380 135 140 145 150 DGNILGNKMEYNYNATNVYI PS-CFP2 DGNILGNKMEYNYNATNVYI g a t g g c a a c a t c c t g g g c a a t a a g a t g g a g t a c a a c t a c a a c g c c a c c a a t g t g t a c a t c c t a c c g t t g t a g g a c c c g t t a t t c t a c c t c a t g t t g a t g t t g c g g t g g t t a c a c a t g t a g

4440 155 160 165 170 VADKARNGIKVNFKVRHNIK PS-CFP2 VADKARNGIKVNFKVRHNIK g t g g c c g a c a a g g c c a g g a a t g g c a t c a a g g t g a a c t t c a a g g t c c g c c a c a a c a t c a a g c a c c g g c t g t t c c g g t c c t t a c c g t a g t t c c a c t t g a a g t t c c a g g c g g t g t t g t a g t t c

4500 175 180 185 190 DGSVQLADHYQQNTPIGDGP PS-CFP2 DGSVQLADHYQQNTPIGDGP g a t g g c a g c g t g c a g c t g g c c g a c c a c t a c c a g c a g a a t a c c c c c a t c g g c g a t g g c c c t c t a c c g t c g c a c g t c g a c c g g c t g g t g a t g g t c g t c t t a t g g g g g t a g c c g c t a c c g g g a

4560 195 200 205 210 VLLPDNHYLSTQSALSKDPN PS-CFP2 VLLPDNHYLSTQSALSKDPN g t g c t g c t g c c c g a t a a c c a c t a c c t g t c c a c c c a g a g c g c c c t g t c c a a g g a c c c c a a c c a c g a c g a c g g g c t a t t g g t g a t g g a c a g g t g g g t c t c g c g g g a c a g g t t c c t g g g g t t g

4620 215 220 225 230 EKRDHMIYLEFVTAAAITHG PS-CFP2 EKRDHMIYLEFVTAAAITHG g a g a a g c g c g a t c a c a t g a t c t a c c t c g a g t t c g t g a c c g c c g c c g c c a t c a c c c a c g g c c t c t t c g c g c t a g t g t a c t a g a t g g a g c t c a a g c a c t g g c g g c g g c g g t a g t g g g t g c c g

NotI 4680 235 MDELYK* PS-CFP2 MDELYK* a t g g a t g a g c t g t a c a a g t a a a g c g g c c g c g a c t c t a g a t c a t a a t c a g c c a t a c c a c a t t a c c t a c t c g a c a t g t t c a t t t c g c c g g c g c t g a g a t c t a g t a t t a g t c g g t a t g g t g t a

4740 t t g t a g a g g t t t t a c t t g c t t t a a a a a a c c t c c c a c a c c t c c c c c t g a a c c t g a a a c a t a a a c a t c t c c a a a a t g a a c g a a a t t t t t t g g a g g g t g t g g a g g g g g a c t t g g a c t t t g t a t

HpaIMfeI 4800 SV40 poly(A) signal a a a t g a a t g c a a t t g t t g t t g t t a a c t t g t t t a t t g c a g c t t a t a a t g g t t a c a a a t a a a t t t a c t t a c g t t a a c a a c a a c a a t t g a a c a a a t a a c g t c g a a t a t t a c c a a t g t t t a t t t

4860 SV40 poly(A) signal G T G G T T EBV-rev C G T T A T C G T A G T G T T T A A A G SV40pA-R g c a a t a g c a t c a c a a a t t t c a c a a a t a a a g c a t t t t t t t c a c t g c a t t c t a g t t g t g g t t c g t t a t c g t a g t g t t t a a a g t g t t t a t t t c g t a a a a a a a g t g a c g t a a g a t c a a c a c c a a

AflII 4920 SV40 poly(A) signal f1 ori T G T C C A A A C T C A T C EBV-rev t g t c c a a a c t c a t c a a t g t a t c t t a a g g c g t a a a t t g t a a g c g t t a a t a t t t t g t t a a a a a c a g g t t t g a g t a g t t a c a t a g a a t t c c g c a t t t a a c a t t c g c a a t t a t a a a a c a a t t t t

4980 f1 ori t t c g c g t t a a a t t t t t g t t a a a t c a g c t c a t t t t t t a a c c a a t a g g c c g a a a t c g g c a a a a a g c g c a a t t t a a a a a c a a t t t a g t c g a g t a a a a a a t t g g t t a t c c g g c t t t a g c c g t t t

5040 f1 ori G G T C A A A C C T T G F1ori-F a t c c c t t a t a a a t c a a a a g a a t a g a c c g a g a t a g g g t t g a g t g t t g t t c c a g t t t g g a a c t a g g g a a t a t t t a g t t t t c t t a t c t g g c t c t a t c c c a a c t c a c a a c a a g g t c a a a c c t t g

5100 f1 ori T T C T C A G G T G F1ori-F a a g a g t c c a c t a t t a a a g a a c g t g g a c t c c a a c g t c a a a g g g c g a a a a a c c g t c t a t c a g t t c t c a g g t g a t a a t t t c t t g c a c c t g a g g t t g c a g t t t c c c g c t t t t t g g c a g a t a g t c

5160 f1 ori g g c g a t g g c c c a c t a c g t g a a c c a t c a c c c t a a t c a a g t t t t t t g g g g t c g a g g t g c c g t c c g c t a c c g g g t g a t g c a c t t g g t a g t g g g a t t a g t t c a a a a a a c c c c a g c t c c a c g g c a

5220 f1 ori a a a g c a c t a a a t c g g a a c c c t a a a g g g a g c c c c c g a t t t a g a g c t t g a c g g g g a a a g c c g t t t c g t g a t t t a g c c t t g g g a t t t c c c t c g g g g g c t a a a t c t c g a a c t g c c c c t t t c g g c

5280 f1 ori A G G G A A G A A A G C G A A A G G A G F1ori-R g c g a a c g t g g c g a g a a a g g a a g g g a a g a a a g c g a a a g g a g c g g g c g c t a g g g c g c t g g c a c g c t t g c a c c g c t c t t t c c t t c c c t t c t t t c g c t t t c c t c g c c c g c g a t c c c g c g a c c g t

5340 f1 ori a g t g t a g c g g t c a c g c t g c g c g t a a c c a c c a c a c c c g c c g c g c t t a a t g c g c c g c t a c a g t c a c a t c g c c a g t g c g a c g c g c a t t g g t g g t g t g g g c g g c g c g a a t t a c g c g g c g a t g t c

5400 f1 ori AmpR promoter g g c g c g t c a g g t g g c a c t t t t c g g g g a a a t g t g c g c g g a a c c c c t a t t t g t t t a t t t t t c c c g c g c a g t c c a c c g t g a a a a g c c c c t t t a c a c g c g c c t t g g g g a t a a a c a a a t a a a a a g

5460 AmpR promoter t a a a t a c a t t c a a a t a t g t a t c c g c t c a t g a g a c a a t a a c c c t g a t a a a t g c t t c a a t a a a t t t a t g t a a g t t t a t a c a t a g g c g a g t a c t c t g t t a t t g g g a c t a t t t a c g a a g t t a t t

5520 AmpR promoter SV40 promoter SV40 enhancer C C T T A C A C A C A G T C A A T C pBABE 3' t a t t g a a a a a g g a a g a g t c c t g a g g c g g a a a g a a c c a g c t g t g g a a t g t g t g t c a g t t a g a t a a c t t t t t c c t t c t c a g g a c t c c g c c t t t c t t g g t c g a c a c c t t a c a c a c a g t c a a t c

5580 SV40 promoter SV40 enhancer C C A pBABE 3' g g t g t g g a a a g t c c c c a g g c t c c c c a g c a g g c a g a a g t a t g c a a a g c a t g c a t c t c a a t t c c a c a c c t t t c a g g g g t c c g a g g g g t c g t c c g t c t t c a t a c g t t t c g t a c g t a g a g t t a a

5640 SV40 promoter SV40 enhancer a g t c a g c a a c c a g g t g t g g a a a g t c c c c a g g c t c c c c a g c a g g c a g a a g t a t g c a a a g c a t c a g t c g t t g g t c c a c a c c t t t c a g g g g t c c g a g g g g t c g t c c g t c t t c a t a c g t t t c g t

5700 SV40 promoter SV40 enhancer SV40 ori t g c a t c t c a a t t a g t c a g c a a c c a t a g t c c c g c c c c t a a c t c c g c c c a t c c c g c c c c t a a a c g t a g a g t t a a t c a g t c g t t g g t a t c a g g g c g g g g a t t g a g g c g g g t a g g g c g g g g a t t

5760 SV40 promoter SV40 ori c t c c g c c c a g t t c c g c c c a t t c t c c g c c c c a t g g c t g a c t a a t t t t t t t t a t t t a t g c a g g a g g c g g g t c a a g g c g g g t a a g a g g c g g g g t a c c g a c t g a t t a a a a a a a a t a a a t a c g t c

5820 SV40 promoter SV40 ori a g g c c g a g g c c g c c t c g g c c t c t g a g c t a t t c c a g a a g t a g t g a g g a g g c t t t t t t g g a g t c c g g c t c c g g c g g a g c c g g a g a c t c g a t a a g g t c t t c a t c a c t c c t c c g a a a a a a c c t c

5880 1 MIE SV40 promoter NeoR/KanR 1 MIE SV40 ori NeoR/KanR MIE g c c t a g g c t t t t g c a a a g a t c g a t c a a g a g a c a g g a t g a g g a t c g t t t c g c a t g a t t g a a c g g a t c c g a a a a c g t t t c t a g c t a g t t c t c t g t c c t a c t c c t a g c a a a g c g t a c t a a c t t

5940 5 10 15 20 QDGLHAGSPAAWVERLFGYD NeoR/KanR 5 10 15 20 QDGLHAGSPAAWVERLFGYD NeoR/KanR G A T A A G C C G A T A C T G Neo-R QDGLHAGSPAAWVERLFGYD c a a g a t g g a t t g c a c g c a g g t t c t c c g g c c g c t t g g g t g g a g a g g c t a t t c g g c t a t g a c g t t c t a c c t a a c g t g c g t c c a a g a g g c c g g c g a a c c c a c c t c t c c g a t a a g c c g a t a c t g

6000 25 30 35 40 WAQQTIGCSDAAVFRLSAQG NeoR/KanR 25 30 35 40 WAQQTIGCSDAAVFRLSAQG NeoR/KanR A C C C G Neo-R WAQQTIGCSDAAVFRLSAQG t g g g c a c a a c a g a c a a t c g g c t g c t c t g a t g c c g c c g t g t t c c g g c t g t c a g c g c a g g g g a c c c g t g t t g t c t g t t a g c c g a c g a g a c t a c g g c g g c a c a a g g c c g a c a g t c g c g t c c c c

6060 45 50 55 60 RPVLFVKTDLSGALNELQDE NeoR/KanR 45 50 55 60 RPVLFVKTDLSGALNELQDE NeoR/KanR RPVLFVKTDLSGALNELQDE c g c c c g g t t c t t t t t g t c a a g a c c g a c c t g t c c g g t g c c c t g a a t g a a c t g c a a g a c g a g g c g g g c c a a g a a a a a c a g t t c t g g c t g g a c a g g c c a c g g g a c t t a c t t g a c g t t c t g c t c

Tth111IPflFIFspI 6120 65 70 75 80 AARLSWLATTGVPCAAVLDV NeoR/KanR 65 70 75 80 AARLSWLATTGVPCAAVLDV NeoR/KanR AARLSWLATTGVPCAAVLDV g c a g c g c g g c t a t c g t g g c t g g c c a c g a c g g g c g t t c c t t g c g c a g c t g t g c t c g a c g t t c g t c g c g c c g a t a g c a c c g a c c g g t g c t g c c c g c a a g g a a c g c g t c g a c a c g a g c t g c a a

6180 85 90 95 100 VTEAGRDWLLLGEVPGQDLL NeoR/KanR 85 90 95 100 VTEAGRDWLLLGEVPGQDLL NeoR/KanR VTEAGRDWLLLGEVPGQDLL g t c a c t g a a g c g g g a a g g g a c t g g c t g c t a t t g g g c g a a g t g c c g g g g c a g g a t c t c c t g c a g t g a c t t c g c c c t t c c c t g a c c g a c g a t a a c c c g c t t c a c g g c c c c g t c c t a g a g g a c

6240 105 110 115 120 SSHLAPAEKVSIMADAMRRL NeoR/KanR 105 110 115 120 SSHLAPAEKVSIMADAMRRL NeoR/KanR SSHLAPAEKVSIMADAMRRL *RVKSRGLFYGDHSICHPPQ t c a t c t c a c c t t g c t c c t g c c g a g a a a g t a t c c a t c a t g g c t g a t g c a a t g c g g c g g c t g a g t a g a g t g g a a c g a g g a c g g c t c t t t c a t a g g t a g t a c c g a c t a c g t t a c g c c g c c g a c

6300 125 130 135 140 HTLDPATCPFDHQAKHRIER NeoR/KanR 125 130 135 140 HTLDPATCPFDHQAKHRIER NeoR/KanR HTLDPATCPFDHQAKHRIER MRKIRSGAWEVVLRFMADLS c a t a c g c t t g a t c c g g c t a c c t g c c c a t t c g a c c a c c a a g c g a a a c a t c g c a t c g a g c g a g t a t g c g a a c t a g g c c g a t g g a c g g g t a a g c t g g t g g t t c g c t t t g t a g c g t a g c t c g c t

6360 145 150 155 160 ARTRMEAGLVDQDDLDEEHQ NeoR/KanR 145 150 155 160 ARTRMEAGLVDQDDLDEEHQ NeoR/KanR ARTRMEAGLVDQDDLDEEHQ CTSPHFGTKDILIIQVFLML g c a c g t a c t c g g a t g g a a g c c g g t c t t g t c g a t c a g g a t g a t c t g g a c g a a g a g c a t c a g c g t g c a t g a g c c t a c c t t c g g c c a g a a c a g c t a g t c c t a c t a g a c c t g c t t c t c g t a g t c

6420 165 170 175 180 GLAPAELFARLKASMPDGED NeoR/KanR 165 170 175 180 GLAPAELFARLKASMPDGED NeoR/KanR GLAPAELFARLKASMPDGED PERWGFQEGPELRAHGVALI g g g c t c g c g c c a g c c g a a c t g t t c g c c a g g c t c a a g g c g a g c a t g c c c g a c g g c g a g g a t c c c g a g c g c g g t c g g c t t g a c a a g c g g t c c g a g t t c c g c t c g t a c g g g c t g c c g c t c c t a

6480 185 190 195 200 LVVTHGDACLPNIMVENGRF NeoR/KanR 185 190 195 200 LVVTHGDACLPNIMVENGRF NeoR/KanR LVVTHGDACLPNIMVENGRF EDHGMAIGAQRIDHHFIAAK c t c g t c g t g a c c c a t g g c g a t g c c t g c t t g c c g a a t a t c a t g g t g g a a a a t g g c c g c t t t g a g c a g c a c t g g g t a c c g c t a c g g a c g a a c g g c t t a t a g t a c c a c c t t t t a c c g g c g a a a

RsrII 6540 205 210 215 220 SGFIDCGRLGVADRYQDIAL NeoR/KanR 205 210 215 220 SGFIDCGRLGVADRYQDIAL NeoR/KanR C G T T G Neo-F SGFIDCGRLGVADRYQDIAL RSEDVTAPQTHRVAILVYRQ t c t g g a t t c a t c g a c t g t g g c c g g c t g g g t g t g g c g g a c c g c t a t c a g g a c a t a g c g t t g a g a c c t a a g t a g c t g a c a c c g g c c g a c c c a c a c c g c c t g g c g a t a g t c c t g t a t c g c a a c

6600 225 230 235 240 ATRDIAEELGGEWADRFLVL NeoR/KanR 225 230 235 240 ATRDIAEELGGEWADRFLVL NeoR/KanR G C T A C C C G T G A T A T T Neo-F ATRDIAEELGGEWADRFLVL SGTINSFLKAAFPSVAEEHK g c t a c c c g t g a t a t t g c t g a a g a g c t t g g c g g c g a a t g g g c t g a c c g c t t c c t c g t g c t t c g a t g g g c a c t a t a a c g a c t t c t c g a a c c g c c g c t t a c c c g a c t g g c g a a g g a g c a c g a a

6660 245 250 255 260 YGIAAPDSQRIAFYRLLDEF NeoR/KanR 245 250 255 260 YGIAAPDSQRIAFYRLLDEF NeoR/KanR YGIAAPDSQRIAFYRLLDEF VTDGSGIRLADGEIAKKVLE t a c g g t a t c g c c g c t c c c g a t t c g c a g c g c a t c g c c t t c t a t c g c c t t c t t g a c g a g t t c a t g c c a t a g c g g c g a g g g c t a a g c g t c g c g t a g c g g a a g a t a g c g g a a g a a c t g c t c a a g

6720 265 F* NeoR/KanR 265 F* NeoR/KanR F* MTDQATPNLPS ESRSEPTRFSRGLSAWGAM t t c t g a g c g g g a c t c t g g g g t t c g a a a t g a c c g a c c a a g c g a c g c c c a a c c t g c c a t c a c a a g a c t c g c c c t g a g a c c c c a a g c t t t a c t g g c t g g t t c g c t g c g g g t t g g a c g g t a g t g

6780 RDFDSTAAFYERLGFGIVFR g a g a t t t c g a t t c c a c c g c c g c c t t c t a t g a a a g g t t g g g c t t c g g a a t c g t t t t c c g g g c t c t a a a g c t a a g g t g g c g g c g g a a g a t a c t t t c c a a c c c g a a g c c t t a g c a a a a g g c c c

6840 DAGWMILQRGDLMLEFFAHP a c g c c g g c t g g a t g a t c c t c c a g c g c g g g g a t c t c a t g c t g g a g t t c t t c g c c c a c c c t a t g c g g c c g a c c t a c t a g g a g g t c g c g c c c c t a g a g t a c g a c c t c a a g a a g c g g g t g g g a t

6900 HSV TK poly(A) signal A C T T T G T G C C T T C C T C T G T T TK-pA-R RGRLTETRKETIPEGTRAMT g g g g g a g g c t a a c t g a a a c a c g g a a g g a g a c a a t a c c g g a a g g a a c c c g c g c t a t g a c g g c c c c c t c c g a t t g a c t t t g t g c c t t c c t c t g t t a t g g c c t t c c t t g g g c g c g a t a c t g c c

6960 HSV TK poly(A) signal AIKRQNKTHGVGSFVHKRGV c a a t a a a a a g a c a g a a t a a a a c g c a c g g t g t t g g g t c g t t t g t t c a t a a a c g c g g g g t t c g t t a t t t t t c t g t c t t a t t t t g c g t g c c a c a a c c c a g c a a a c a a g t a t t t g c g c c c c a a g

7020 RSQGWHSVDTPPRPHWGQYA g g t c c c a g g g c t g g c a c t c t g t c g a t a c c c c a c c g a g a c c c c a t t g g g g c c a a t a c g c c c c c a g g g t c c c g a c c g t g a g a c a g c t a t g g g g t g g c t c t g g g g t a a c c c c g g t t a t g c g g g

7080 RVSSFSPPHPPSSGEGPGLA g c g t t t c t t c c t t t t c c c c a c c c c a c c c c c c a a g t t c g g g t g a a g g c c c a g g g c t c g c a g c g c a a a g a a g g a a a a g g g g t g g g g t g g g g g g t t c a a g c c c a c t t c c g g g t c c c g a g c g t c

7140 ANVGAAGPAIASGYSYIL* c c a a c g t c g g g g c g g c a g g c c c t g c c a t a g c c t c a g g t t a c t c a t a t a t a c t t t a g a t t g g g t t g c a g c c c c g c c g t c c g g g a c g g t a t c g g a g t c c a a t g a g t a t a t a t g a a a t c t a a c

7200 a t t t a a a a c t t c a t t t t t a a t t t a a a a g g a t c t a g g t g a a g a t c c t t t t t g a t a a t c t c a t a a a t t t t g a a g t a a a a a t t a a a t t t t c c t a g a t c c a c t t c t a g g a a a a a c t a t t a g a g t

7260 t g a c c a a a a t c c c t t a a c g t g a g t t t t c g t t c c a c t g a g c g t c a g a c c c c g t a g a a a a g a a c t g g t t t t a g g g a a t t g c a c t c a a a a g c a a g g t g a c t c g c a g t c t g g g g c a t c t t t t c t

7320 ori t c a a a g g a t c t t c t t g a g a t c c t t t t t t t c t g c g c g t a a t c t g c t g c t t g c a a a c a a a a a a g t t t c c t a g a a g a a c t c t a g g a a a a a a a g a c g c g c a t t a g a c g a c g a a c g t t t g t t t t t

7380 ori a a c c a c c g c t a c c a g c g g t g g t t t g t t t g c c g g a t c a a g a g c t a c c a a c t c t t t t t c c g a t t g g t g g c g a t g g t c g c c a c c a a a c a a a c g g c c t a g t t c t c g a t g g t t g a g a a a a a g g c t

7440 ori a g g t a a c t g g c t t c a g c a g a g c g c a g a t a c c a a a t a c t g t t c t t c t a g t g t a g c c g t a g t t c c a t t g a c c g a a g t c g t c t c g c g t c t a t g g t t t a t g a c a a g a a g a t c a c a t c g g c a t c a

7500 ori t a g g c c a c c a c t t c a a g a a c t c t g t a g c a c c g c c t a c a t a c c t c g c t c t g c t a a t c c t g t a t c c g g t g g t g a a g t t c t t g a g a c a t c g t g g c g g a t g t a t g g a g c g a g a c g a t t a g g a c a

7560 ori t a c c a g t g g c t g c t g c c a g t g g c g a t a a g t c g t g t c t t a c c g g g t t g g a c t c a a g a c g a t a t g g t c a c c g a c g a c g g t c a c c g c t a t t c a g c a c a g a a t g g c c c a a c c t g a g t t c t g c t a

7620 ori a g t t a c c g g a t a a g g c g c a g c g g t c g g g c t g a a c g g g g g g t t c g t g c a c a c a g c c c a g c t t c a a t g g c c t a t t c c g c g t c g c c a g c c c g a c t t g c c c c c c a a g c a c g t g t g t c g g g t c g a

7680 ori t g g a g c g a a c g a c c t a c a c c g a a c t g a g a t a c c t a c a g c g t g a g c t a t g a g a a a g c g c c a a c c t c g c t t g c t g g a t g t g g c t t g a c t c t a t g g a t g t c g c a c t c g a t a c t c t t t c g c g g t

7740 ori c g c t t c c c g a a g g g a g a a a g g c g g a c a g g t a t c c g g t a a g c g g c a g g g t c g g a a c a g g a g g c g a a g g g c t t c c c t c t t t c c g c c t g t c c a t a g g c c a t t c g c c g t c c c a g c c t t g t c c t c

7800 ori G G G A A A C G C C T G G T A T C T T T pBR322ori-F a g c g c a c g a g g g a g c t t c c a g g g g g a a a c g c c t g g t a t c t t t a t a g t c c t g t c g g g t t t c t c g c g t g c t c c c t c g a a g g t c c c c c t t t g c g g a c c a t a g a a a t a t c a g g a c a g c c c a a a g

7860 ori g c c a c c t c t g a c t t g a g c g t c g a t t t t t g t g a t g c t c g t c a g g g g g g c g g a g c c t a t g g a c g g t g g a g a c t g a a c t c g c a g c t a a a a a c a c t a c g a g c a g t c c c c c c g c c t c g g a t a c c t

3ʹ 5ʹ 7918 ori a a a a c g c c a g c a a c g c g g c c t t t t t a c g g t t c c t g g c c t t t t g c t g g c c t t t t g c t c a t t t t g c g g t c g t t g c g c c g g a a a a a t g c c a a g g a c c g g a a a a c g a c c g g a a a a c g a g t

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.