Addgene Opto-GPR120 Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: Opto-GPR120 Sequencing Result


Map View

pRS-marker (6485 .. 6504) BglII (6453) SgrDI (6439) SspI (6322) Amp-R (6068 .. 6087) PvuI (5888) AhdI (5518) pBR322ori-F (4766 .. 4785) L4440 (4515 .. 4532) CAP binding site M13/pUC Reverse (4297 .. 4319) lac operator M13 Reverse (4284 .. 4300) BstZ17I (4246) EBV-rev (4205 .. 4224) BsmI (4194) SV40pA-R (4151 .. 4170) BstBI (3956) Neo-F (3810 .. 3829) RsrII (3790) PflFI - Tth111I (3392) MfeI (0) NruI (47) MluI (67) SpeI (88) CMV enhancer NdeI (323) SnaBI (429) CMV-F (608 .. 628) Eco53kI (655) SacI (657) T7 (702 .. 721) NheI (734) BmtI (738) AflII (747) HindIII (750) BsrGI (811) AscI (1041) SacII (1058) BsgI (1131) PaqCI (1282) PpuMI * (1293) BstXI (1979) PaeR7I - PspXI - XhoI (1995) XbaI (2001) BGH-rev (2032 .. 2049) BbsI (2227) F1ori-R (2395 .. 2414) DraIII (2541) F1ori-F (2605 .. 2626) pBABE 3' (2745 .. 2765) CsiI - SexAI * (2831) SV40pro-F (2992 .. 3011) BseRI (3060) StuI (3063) AvrII (3064) TspMI - XmaI (3085) SmaI (3087) BsaBI * (3133) EagI (3180) Neo-R (3200 .. 3219) Opto-GPR120 6602 bp

Sequence View

NruIMfeI 5ʹ 3ʹ 60 a a t t g c a t g a a g a a t c t g c t t a g g g t t a g g c g t t t t g c g c t g c t t c g c g a t g t a c g g g c c t t a a c g t a c t t c t t a g a c g a a t c c c a a t c c g c a a a a c g c g a c g a a g c g c t a c a t g c c c g g

SpeIMluI 120 CMV enhancer a g a t a t a c g c g t t g a c a t t g a t t a t t g a c t a g t t a t t a a t a g t a a t c a a t t a c g g g g t c a t c t a t a t g c g c a a c t g t a a c t a a t a a c t g a t c a a t a a t t a t c a t t a g t t a a t g c c c c a g t

180 CMV enhancer t t a g t t c a t a g c c c a t a t a t g g a g t t c c g c g t t a c a t a a c t t a c g g t a a a t g g c c c g c c t a a t c a a g t a t c g g g t a t a t a c c t c a a g g c g c a a t g t a t t g a a t g c c a t t t a c c g g g c g g a

240 CMV enhancer g g c t g a c c g c c c a a c g a c c c c c g c c c a t t g a c g t c a a t a a t g a c g t a t g t t c c c a t a g t a c c g a c t g g c g g g t t g c t g g g g g c g g g t a a c t g c a g t t a t t a c t g c a t a c a a g g g t a t c a t

300 CMV enhancer a c g c c a a t a g g g a c t t t c c a t t g a c g t c a a t g g g t g g a g t a t t t a c g g t a a a c t g c c c a c t g c g g t t a t c c c t g a a a g g t a a c t g c a g t t a c c c a c c t c a t a a a t g c c a t t t g a c g g g t g

NdeI 360 CMV enhancer t t g g c a g t a c a t c a a g t g t a t c a t a t g c c a a g t a c g c c c c c t a t t g a c g t c a a t g a c g g t a a c c g t c a t g t a g t t c a c a t a g t a t a c g g t t c a t g c g g g g g a t a a c t g c a g t t a c t g c c a

420 CMV enhancer a a a t g g c c c g c c t g g c a t t a t g c c c a g t a c a t g a c c t t a t g g g a c t t t c c t a c t t g g c a g t t t a c c g g g c g g a c c g t a a t a c g g g t c a t g t a c t g g a a t a c c c t g a a a g g a t g a a c c g t c

SnaBI 480 CMV enhancer CMV promoter t a c a t c t a c g t a t t a g t c a t c g c t a t t a c c a t g g t g a t g c g g t t t t g g c a g t a c a t c a a t a t g t a g a t g c a t a a t c a g t a g c g a t a a t g g t a c c a c t a c g c c a a a a c c g t c a t g t a g t t a

540 CMV promoter g g g c g t g g a t a g c g g t t t g a c t c a c g g g g a t t t c c a a g t c t c c a c c c c a t t g a c g t c a a t c c c g c a c c t a t c g c c a a a c t g a g t g c c c c t a a a g g t t c a g a g g t g g g g t a a c t g c a g t t a

600 CMV promoter g g g a g t t t g t t t t g g c a c c a a a a t c a a c g g g a c t t t c c a a a a t g t c g t a a c a a c t c c g c c c c c t c a a a c a a a a c c g t g g t t t t a g t t g c c c t g a a a g g t t t t a c a g c a t t g t t g a g g c g g

Eco53kISacI 660 CMV promoter C G C A A A T G G G C G G T A G G C G T G CMV-F c c a t t g a c g c a a a t g g g c g g t a g g c g t g t a c g g t g g g a g g t c t a t a t a a g c a g a g c t c t c g g t a a c t g c g t t t a c c c g c c a t c c g c a c a t g c c a c c c t c c a g a t a t a t t c g t c t c g a g a g

720 T7 promoter T A A T A C G A C T C A C T A T A G G T7 t g g c t a a c t a g a g a a c c c a c t g c t t a c t g g c t t a t c g a a a t t a a t a c g a c t c a c t a t a g g a c c g a t t g a t c t c t t g g g t g a c g a a t g a c c g a a t a g c t t t a a t t a t g c t g a g t g a t a t c c

AflIIHindIIIBmtINheI 780 G T7 MKTIIA g a g a c c c a a g c t g g c t a g c g t t t a a a c t t a a g c t t g c c a c c a t g a a a a c g a t c a t c g c c c c t c t g g g t t c g a c c g a t c g c a a a t t t g a a t t c g a a c g g t g g t a c t t t t g c t a g t a g c g g g

BsrGI 840 1 5 YTDIEMNRL VSV-G tag LSYIFCLVFAMYTDIEMNRL t g a g c t a c a t c t t c t g c c t g g t a t t c g c c a t g t a c a c c g a t a t a g a g a t g a a c a g g c t g g a c t c g a t g t a g a a g a c g g a c c a t a a g c g g t a c a t g t g g c t a t a t c t c t a c t t g t c c g a c c

900 10 GK DSLMNGTE 10 15 GPNFYVPFSN VSV-G tag opsin tag GKDSLMNGTEGPNFYVPFSN g a a a g g a t a g c c t c a t g a a c g g g a c c g a g g g c c c a a a c t t c t a c g t g c c t t t c t c c a a c a c t t t c c t a t c g g a g t a c t t g c c c t g g c t c c c g g g t t t g a a g a t g c a c g g a a a g a g g t t g t

960 KTG (in frame with opsin tag) VVRSPFEAPQYYLAEPW opsin tag KTGVVRSPFEAPQYYLAEPW a g a c g g g c g t g g t g c g c a g c c c c t t c g a g g c c c c g c a g t a c t a c c t g g c g g a g c c a t g g c t c t g c c c g c a c c a c g c g t c g g g g a a g c t c c g g g g c g t c a t g a t g g a c c g c c t c g g t a c c g

1020 (in frame with opsin tag) QFSMLAAYMFLLIMLGFPIN QFSMLAAYMFLLIMLGFPIN a g t t c t c c a t g c t g g c c g c c t a c a t g t t c c t g c t g a t c a t g c t t g g c t t c c c c a t c a a c t t c a a g a g g t a c g a c c g g c g g a t g t a c a a g g a c g a c t a g t a c g a a c c g a a g g g g t a g t t g a

SacIIAscI 1080 (in frame with opsin tag) FLTLYVVARRRRRGALNYIL FLTLYVVARRRRRGALNYIL t c c t c a c g c t g t a c g t c g t g g c g c g c c g a c g a c g c c g c g g c g c g c t c a a c t a c a t c c t g c a g g a g t g c g a c a t g c a g c a c c g c g c g g c t g c t g c g g c g c c g c g c g a g t t g a t g t a g g a c g

BsgI 1140 (in frame with opsin tag) LNLAVADLFMVFGGFTTTLY LNLAVADLFMVFGGFTTTLY t c a a c c t g g c c g t g g c c g a c c t c t t c a t g g t g t t c g g g g g c t t c a c c a c c a c c c t c t a c a a g t t g g a c c g g c a c c g g c t g g a g a a g t a c c a c a a g c c c c c g a a g t g g t g g t g g g a g a t g t

1200 (in frame with opsin tag) TSLHGYFVFGPTGCNLEGFF TSLHGYFVFGPTGCNLEGFF c c t c t c t g c a c g g g t a c t t c g t c t t t g g g c c c a c g g g c t g c a a c c t g g a g g g c t t c t t t g g g a g a g a c g t g c c c a t g a a g c a g a a a c c c g g g t g c c c g a c g t t g g a c c t c c c g a a g a a a c

1260 (in frame with opsin tag) ATLGGEIALWSLVVLAIERY ATLGGEIALWSLVVLAIERY c c a c c t t g g g c g g t g a a a t t g c a c t g t g g t c c t t g g t g g t c c t g g c c a t c g a g c g g t a c g g g t g g a a c c c g c c a c t t t a a c g t g a c a c c a g g a a c c a c c a g g a c c g g t a g c t c g c c a t g c

PpuMI*PaqCI 1320 (in frame with opsin tag) VVVVHLQRGVRGPGRRAAIM VVVVHLQRGVRGPGRRAAIM t g g t g g t g g t g c a c c t g c a g c g c g g c g t g c g g g g t c c t g g g c g g c g g g c g g c c a t c a t g g a c c a c c a c c a c g t g g a c g t c g c g c c g c a c g c c c c a g g a c c c g c c g c c c g c c g g t a g t a c c

1380 (in frame with opsin tag) GVAFTWVMALACAAPPLVGW GVAFTWVMALACAAPPLVGW g c g t c g c c t t c a c c t g g g t c a t g g c t c t g g c c t g t g c c g c g c c c c c c c t c g t c g g c t g g t c g c a g c g g a a g t g g a c c c a g t a c c g a g a c c g g a c a c g g c g c g g g g g g g a g c a g c c g a c c a

1440 (in frame with opsin tag) SRYIPEGMQCSCGIDYYTPH SRYIPEGMQCSCGIDYYTPH c c a g g t a c a t c c c g g a g g g c a t g c a g t g c t c g t g c g g g a t t g a c t a c t a c a c g c c c c a c g g g t c c a t g t a g g g c c t c c c g t a c g t c a c g a g c a c g c c c t a a c t g a t g a t g t g c g g g g t g c

1500 (in frame with opsin tag) EETNNESFVIYMFVVHFIIP EETNNESFVIYMFVVHFIIP a g g a a a c c a a c a a t g a g t c g t t c g t c a t c t a c a t g t t c g t g g t c c a c t t c a t c a t c c c c c t c c t t t g g t t g t t a c t c a g c a a g c a g t a g a t g t a c a a g c a c c a g g t g a a g t a g t a g g g g g

1560 (in frame with opsin tag) LIVIFFCYGKILQTSEHLLD LIVIFFCYGKILQTSEHLLD t g a t t g t c a t a t t c t t c t g c t a c g g a a a a a t t t t a c a g a c c t c g g a a c a c c t c c t g g a t g a c t a a c a g t a t a a g a a g a c g a t g c c t t t t t a a a a t g t c t g g a g c c t t g t g g a g g a c c t a c

1620 (in frame with opsin tag) ARAVVTHSEITKASRKRLTV ARAVVTHSEITKASRKRLTV c a a g a g c t g t c g t g a c t c a c a g t g a g a t c a c a a a g g c a t c a a g g a a g a g g c t c a c g g t a a g t t c t c g a c a g c a c t g a g t g t c a c t c t a g t g t t t c c g t a g t t c c t t c t c c g a g t g c c a t t

1680 (in frame with opsin tag) SLAYSESHQIRVSQQDFRLF SLAYSESHQIRVSQQDFRLF g c c t g g c c t a c t c g g a g a g c c a c c a g a t c c g c g t g t c c c a g c a g g a c t t c c g g c t g t t c c c g g a c c g g a t g a g c c t c t c g g t g g t c t a g g c g c a c a g g g t c g t c c t g a a g g c c g a c a a g g

1740 (in frame with opsin tag) RMVIIMVIAFLICWLPYAGV RMVIIMVIAFLICWLPYAGV g c a t g g t g a t c a t c a t g g t c a t c g c t t t c c t a a t c t g c t g g c t g c c c t a c g c t g g g g t g g c g t a c c a c t a g t a g t a c c a g t a g c g a a a g g a t t a g a c g a c c g a c g g g a t g c g a c c c c a c c

1800 (in frame with opsin tag) AFYIFTHQGSDFGPIFMTIP AFYIFTHQGSDFGPIFMTIP c g t t c t a c a t c t t c a c c c a t c a g g g c t c t g a c t t t g g c c c c a t c t t c a t g a c c a t c c c g g g c a a g a t g t a g a a g t g g g t a g t c c c g a g a c t g a a a c c g g g g t a g a a g t a c t g g t a g g g c c

1860 (in frame with opsin tag) AFFAKTSAVYNPVIYIMMNK AFFAKTSAVYNPVIYIMMNK c t t t c t t t g c c a a g a c t t c t g c c g t c t a c a a c c c c g t c a t c t a c a t c a t g a t g a a c a a g c g a a a g a a a c g g t t c t g a a g a c g g c a g a t g t t g g g g c a g t a g a t g t a g t a c t a c t t g t t c g

1920 (in frame with opsin tag) QFRWKKIFCCFWFPEKGAIL QFRWKKIFCCFWFPEKGAIL a g t t c c g c t g g a a g a a a a t t t t t t g c t g c t t c t g g t t c c c a g a a a a g g g a g c c a t t t t a a t c a a g g c g a c c t t c t t t t a a a a a a c g a c g a a g a c c a a g g g t c t t t t c c c t c g g t a a a a t t

BstXI 1980 (in frame with opsin tag) TDTSVKRNDLSIISGTETSQ TDTSVKRNDLSIISGTETSQ c a g a c a c a t c t g t c a a a a g a a a t g a c t t g t c g a t t a t t t c t g g c a c a g a a a c c a g c c a a g g t c t g t g t a g a c a g t t t t c t t t a c t g a a c a g c t a a t a a a g a c c g t g t c t t t g g t c g g t t c

XbaIPaeR7IPspXIXhoI 2040 VAPA* bGH poly(A) signal G G A G C T G A C BGH-rev VAPA* t g g c g c c t g c c t a a c t c g a g t c t a g a g g g c c c g t t t a a a c c c g c t g a t c a g c c t c g a c t g a c c g c g g a c g g a t t g a g c t c a g a t c t c c c g g g c a a a t t t g g g c g a c t a g t c g g a g c t g a c

2100 bGH poly(A) signal A C G G A A G A T BGH-rev t g c c t t c t a g t t g c c a g c c a t c t g t t g t t t g c c c c t c c c c c g t g c c t t c c t t g a c c c t g g a c g g a a g a t c a a c g g t c g g t a g a c a a c a a a c g g g g a g g g g g c a c g g a a g g a a c t g g g a c c

2160 bGH poly(A) signal a a g g t g c c a c t c c c a c t g t c c t t t c c t a a t a a a a t g a g g a a a t t g c a t c g c a t t g t c t g a t t c c a c g g t g a g g g t g a c a g g a a a g g a t t a t t t t a c t c c t t t a a c g t a g c g t a a c a g a c t

2220 bGH poly(A) signal g t a g g t g t c a t t c t a t t c t g g g g g g t g g g g t g g g g c a g g a c a g c a a g g g g g a g g a t t g g g c a t c c a c a g t a a g a t a a g a c c c c c c a c c c c a c c c c g t c c t g t c g t t c c c c c t c c t a a c c c

BbsI 2280 bGH poly(A) signal a a g a c a a t a g c a g g c a t g c t g g g g a t g c g g t g g g c t c t a t g g c t t c t g a g g c g g a a a g a a t t c t g t t a t c g t c c g t a c g a c c c c t a c g c c a c c c g a g a t a c c g a a g a c t c c g c c t t t c t t

2340 f1 ori c c a g c t g g g g c t c t a g g g g g t a t c c c c a c g c g c c c t g t a g c g g c g c a t t a a g c g c g g c g g g g t c g a c c c c g a g a t c c c c c a t a g g g g t g c g c g g g a c a t c g c c g c g t a a t t c g c g c c g c c

2400 f1 ori G A G G A A F1ori-R g t g t g g t g g t t a c g c g c a g c g t g a c c g c t a c a c t t g c c a g c g c c c t a g c g c c c g c t c c t t c a c a c c a c c a a t g c g c g t c g c a c t g g c g a t g t g a a c g g t c g c g g g a t c g c g g g c g a g g a a

2460 f1 ori A G C G A A A G A A G G G A F1ori-R t c g c t t t c t t c c c t t c c t t t c t c g c c a c g t t c g c c g g c t t t c c c c g t c a a g c t c t a a a t c a g c g a a a g a a g g g a a g g a a a g a g c g g t g c a a g c g g c c g a a a g g g g c a g t t c g a g a t t t a g

2520 f1 ori g g g g g c t c c c t t t a g g g t t c c g a t t t a g t g c t t t a c g g c a c c t c g a c c c c a a a a a a c t t g c c c c c g a g g g a a a t c c c a a g g c t a a a t c a c g a a a t g c c g t g g a g c t g g g g t t t t t t g a a c

DraIII 2580 f1 ori a t t a g g g t g a t g g t t c a c g t a g t g g g c c a t c g c c c t g a t a g a c g g t t t t t c g c c c t t t g a t a a t c c c a c t a c c a a g t g c a t c a c c c g g t a g c g g g a c t a t c t g c c a a a a a g c g g g a a a c t

2640 f1 ori G T G G A C T C T T G T T C C A A A C T G G F1ori-F c g t t g g a g t c c a c g t t c t t t a a t a g t g g a c t c t t g t t c c a a a c t g g a a c a a c a c t c a a c c g c a a c c t c a g g t g c a a g a a a t t a t c a c c t g a g a a c a a g g t t t g a c c t t g t t g t g a g t t g g

2700 f1 ori c t a t c t c g g t c t a t t c t t t t g a t t t a t a a g g g a t t t t g c c g a t t t c g g c c t a t t g g t t a a g a t a g a g c c a g a t a a g a a a a c t a a a t a t t c c c t a a a a c g g c t a a a g c c g g a t a a c c a a t t

2760 f1 ori SV40 promoter C C T T A C A C A C A G T C A A pBABE 3' a a a a t g a g c t g a t t t a a c a a a a a t t t a a c g c g a a t t a a t t c t g t g g a a t g t g t g t c a g t t t t t t a c t c g a c t a a a t t g t t t t t a a a t t g c g c t t a a t t a a g a c a c c t t a c a c a c a g t c a a

2820 SV40 promoter T C C C A pBABE 3' a g g g t g t g g a a a g t c c c c a g g c t c c c c a g c a g g c a g a a g t a t g c a a a g c a t g c a t c t c a a t c c c a c a c c t t t c a g g g g t c c g a g g g g t c g t c c g t c t t c a t a c g t t t c g t a c g t a g a g t t

SexAI*CsiI 2880 SV40 promoter t t a g t c a g c a a c c a g g t g t g g a a a g t c c c c a g g c t c c c c a g c a g g c a g a a g t a t g c a a a g a a t c a g t c g t t g g t c c a c a c c t t t c a g g g g t c c g a g g g g t c g t c c g t c t t c a t a c g t t t c

2940 SV40 promoter SV40 ori c a t g c a t c t c a a t t a g t c a g c a a c c a t a g t c c c g c c c c t a a c t c c g c c c a t c c c g c c c c t g t a c g t a g a g t t a a t c a g t c g t t g g t a t c a g g g c g g g g a t t g a g g c g g g t a g g g c g g g g a

3000 SV40 promoter SV40 ori T A T T T A T G C SV40pro-F a a c t c c g c c c a g t t c c g c c c a t t c t c c g c c c c a t g g c t g a c t a a t t t t t t t t a t t t a t g c t t g a g g c g g g t c a a g g c g g g t a a g a g g c g g g g t a c c g a c t g a t t a a a a a a a a t a a a t a c g

BseRI 3060 SV40 promoter SV40 ori A G A G G C C G A G G SV40pro-F a g a g g c c g a g g c c g c c t c t g c c t c t g a g c t a t t c c a g a a g t a g t g a g g a g g c t t t t t t g g t c t c c g g c t c c g g c g g a g a c g g a g a c t c g a t a a g g t c t t c a t c a c t c c t c c g a a a a a a c c

TspMIXmaISmaIStuIAvrII 3120 SV40 promoter SV40 ori a g g c c t a g g c t t t t g c a a a a a g c t c c c g g g a g c t t g t a t a t c c a t t t t c g g a t c t g a t c a t c c g g a t c c g a a a a c g t t t t t c g a g g g c c c t c g a a c a t a t a g g t a a a a g c c t a g a c t a g t

BsaBI* 3180 1 5 10 MIEQDGLHAGSP NeoR/KanR MIEQDGLHAGSP a g a g a c a g g a t g a g g a t c g t t t c g c a t g a t t g a a c a a g a t g g a t t g c a c g c a g g t t c t c c t c t c t g t c c t a c t c c t a g c a a a g c g t a c t a a c t t g t t c t a c c t a a c g t g c g t c c a a g a g g

EagI 3240 15 20 25 30 AAWVERLFGYDWAQQTIGCS NeoR/KanR G A T A A G C C G A T A C T G A C C C G Neo-R AAWVERLFGYDWAQQTIGCS g g c c g c t t g g g t g g a g a g g c t a t t c g g c t a t g a c t g g g c a c a a c a g a c a a t c g g c t g c t c c c g g c g a a c c c a c c t c t c c g a t a a g c c g a t a c t g a c c c g t g t t g t c t g t t a g c c g a c g a g

3300 35 40 45 50 DAAVFRLSAQGRPVLFVKTD NeoR/KanR DAAVFRLSAQGRPVLFVKTD t g a t g c c g c c g t g t t c c g g c t g t c a g c g c a g g g g c g c c c g g t t c t t t t t g t c a a g a c c g a a c t a c g g c g g c a c a a g g c c g a c a g t c g c g t c c c c g c g g g c c a a g a a a a a c a g t t c t g g c t

3360 55 60 65 70 LSGALNELQDEAARLSWLAT NeoR/KanR LSGALNELQDEAARLSWLAT c c t g t c c g g t g c c c t g a a t g a a c t g c a g g a c g a g g c a g c g c g g c t a t c g t g g c t g g c c a c g g a c a g g c c a c g g g a c t t a c t t g a c g t c c t g c t c c g t c g c g c c g a t a g c a c c g a c c g g t g

Tth111IPflFI 3420 75 80 85 90 TGVPCAAVLDVVTEAGRDWL NeoR/KanR TGVPCAAVLDVVTEAGRDWL g a c g g g c g t t c c t t g c g c a g c t g t g c t c g a c g t t g t c a c t g a a g c g g g a a g g g a c t g g c t c t g c c c g c a a g g a a c g c g t c g a c a c g a g c t g c a a c a g t g a c t t c g c c c t t c c c t g a c c g a

3480 95 100 105 110 LLGEVPGQDLLSSHLAPAEK NeoR/KanR LLGEVPGQDLLSSHLAPAEK *RVKSRGLF g c t a t t g g g c g a a g t g c c g g g g c a g g a t c t c c t g t c a t c t c a c c t t g c t c c t g c c g a g a a c g a t a a c c c g c t t c a c g g c c c c g t c c t a g a g g a c a g t a g a g t g g a a c g a g g a c g g c t c t t

3540 115 120 125 130 VSIMADAMRRLHTLDPATCP NeoR/KanR VSIMADAMRRLHTLDPATCP YGDHSICHPPQMRKIRSGAW a g t a t c c a t c a t g g c t g a t g c a a t g c g g c g g c t g c a t a c g c t t g a t c c g g c t a c c t g c c c t c a t a g g t a g t a c c g a c t a c g t t a c g c c g c c g a c g t a t g c g a a c t a g g c c g a t g g a c g g g

3600 135 140 145 150 FDHQAKHRIERARTRMEAGL NeoR/KanR FDHQAKHRIERARTRMEAGL EVVLRFMADLSCTSPHFGTK a t t c g a c c a c c a a g c g a a a c a t c g c a t c g a g c g a g c a c g t a c t c g g a t g g a a g c c g g t c t t a a g c t g g t g g t t c g c t t t g t a g c g t a g c t c g c t c g t g c a t g a g c c t a c c t t c g g c c a g a

3660 155 160 165 170 VDQDDLDEEHQGLAPAELFA NeoR/KanR VDQDDLDEEHQGLAPAELFA DILIIQVFLMLPERWGFQEG t g t c g a t c a g g a t g a t c t g g a c g a a g a g c a t c a g g g g c t c g c g c c a g c c g a a c t g t t c g c a c a g c t a g t c c t a c t a g a c c t g c t t c t c g t a g t c c c c g a g c g c g g t c g g c t t g a c a a g c g

3720 175 180 185 190 RLKARMPDGEDLVVTHGDAC NeoR/KanR RLKARMPDGEDLVVTHGDAC PELRAHGVALIEDHGMAIGA c a g g c t c a a g g c g c g c a t g c c c g a c g g c g a g g a t c t c g t c g t g a c c c a t g g c g a t g c c t g g t c c g a g t t c c g c g c g t a c g g g c t g c c g c t c c t a g a g c a g c a c t g g g t a c c g c t a c g g a c

3780 195 200 205 210 LPNIMVENGRFSGFIDCGRL NeoR/KanR LPNIMVENGRFSGFIDCGRL QRIDHHFIAAKRSEDVTAPQ c t t g c c g a a t a t c a t g g t g g a a a a t g g c c g c t t t t c t g g a t t c a t c g a c t g t g g c c g g c t g a a c g g c t t a t a g t a c c a c c t t t t a c c g g c g a a a a g a c c t a a g t a g c t g a c a c c g g c c g a

RsrII 3840 215 220 225 230 GVADRYQDIALATRDIAEEL NeoR/KanR C G T T G G C T A C C C G T G A T A T T Neo-F GVADRYQDIALATRDIAEEL THRVAILVYRQSGTINSFLK g g g t g t g g c g g a c c g c t a t c a g g a c a t a g c g t t g g c t a c c c g t g a t a t t g c t g a a g a g c t c c c a c a c c g c c t g g c g a t a g t c c t g t a t c g c a a c c g a t g g g c a c t a t a a c g a c t t c t c g a

3900 235 240 245 250 GGEWADRFLVLYGIAAPDSQ NeoR/KanR GGEWADRFLVLYGIAAPDSQ AAFPSVAEEHKVTDGSGIRL t g g c g g c g a a t g g g c t g a c c g c t t c c t c g t g c t t t a c g g t a t c g c c g c t c c c g a t t c g c a a c c g c c g c t t a c c c g a c t g g c g a a g g a g c a c g a a a t g c c a t a g c g g c g a g g g c t a a g c g t

BstBI 3960 255 260 265 RIAFYRLLDEFF* NeoR/KanR RIAFYRLLDEFF* ADGEIAKKVLEESRSEPTRF g c g c a t c g c c t t c t a t c g c c t t c t t g a c g a g t t c t t c t g a g c g g g a c t c t g g g g t t c g a a c g c g t a g c g g a a g a t a g c g g a a g a a c t g c t c a a g a a g a c t c g c c c t g a g a c c c c a a g c t t

4020 SRGLSAWGAM a t g a c c g a c c a a g c g a c g c c c a a c c t g c c a t c a c g a g a t t t c g a t t c c a c c g c c g c c t t c t a c t g g c t g g t t c g c t g c g g g t t g g a c g g t a g t g c t c t a a a g c t a a g g t g g c g g c g g a a g

4080 t a t g a a a g g t t g g g c t t c g g a a t c g t t t t c c g g g a c g c c g g c t g g a t g a t c c t c c a g c g c a t a c t t t c c a a c c c g a a g c c t t a g c a a a a g g c c c t g c g g c c g a c c t a c t a g g a g g t c g c g

4140 SV40 poly(A) signal g g g g a t c t c a t g c t g g a g t t c t t c g c c c a c c c c a a c t t g t t t a t t g c a g c t t a t a a t g g t c c c c t a g a g t a c g a c c t c a a g a a g c g g g t g g g g t t g a a c a a a t a a c g t c g a a t a t t a c c a

BsmI 4200 SV40 poly(A) signal C G T T A T C G T A G T G T T T A A A G SV40pA-R t a c a a a t a a a g c a a t a g c a t c a c a a a t t t c a c a a a t a a a g c a t t t t t t t c a c t g c a t t c t a t g t t t a t t t c g t t a t c g t a g t g t t t a a a g t g t t t a t t t c g t a a a a a a a g t g a c g t a a g a

BstZ17I 4260 SV40 poly(A) signal G T G G T T T G T C C A A A C T C A T C EBV-rev a g t t g t g g t t t g t c c a a a c t c a t c a a t g t a t c t t a t c a t g t c t g t a t a c c g t c g a c c t c t t c a a c a c c a a a c a g g t t t g a g t a g t t a c a t a g a a t a g t a c a g a c a t a t g g c a g c t g g a g a

4320 M13 rev lac operator C A G T A T C G A C A A A G G A C M13 Reverse G G A C A C A C T T T A A C A A T A G G C G A M13/pUC Reverse a g c t a g a g c t t g g c g t a a t c a t g g t c a t a g c t g t t t c c t g t g t g a a a t t g t t a t c c g c t c t c g a t c t c g a a c c g c a t t a g t a c c a g t a t c g a c a a a g g a c a c a c t t t a a c a a t a g g c g a g

4380 -10 -35 lac operator lac promoter CAP binding site a c a a t t c c a c a c a a c a t a c g a g c c g g a a g c a t a a a g t g t a a a g c c t g g g g t g c c t a a t g a t g t t a a g g t g t g t t g t a t g c t c g g c c t t c g t a t t t c a c a t t t c g g a c c c c a c g g a t t a c t

4440 CAP binding site g t g a g c t a a c t c a c a t t a a t t g c g t t g c g c t c a c t g c c c g c t t t c c a g t c g g g a a a c c t g c a c t c g a t t g a g t g t a a t t a a c g c a a c g c g a g t g a c g g g c g a a a g g t c a g c c c t t t g g a c

4500 t c g t g c c a g c t g c a t t a a t g a a t c g g c c a a c g c g c g g g g a g a g g c g g t t t g c g t a t t g g g a g c a c g g t c g a c g t a a t t a c t t a g c c g g t t g c g c g c c c c t c t c c g c c a a a c g c a t a a c c c

4560 G A G C G A G T G A C T G A G C G A L4440 c g c t c t t c c g c t t c c t c g c t c a c t g a c t c g c t g c g c t c g g t c g t t c g g c t g c g g c g a g c g g c g a g a a g g c g a a g g a g c g a g t g a c t g a g c g a c g c g a g c c a g c a a g c c g a c g c c g c t c g c

4620 g t a t c a g c t c a c t c a a a g g c g g t a a t a c g g t t a t c c a c a g a a t c a g g g g a t a a c g c a g g a c a t a g t c g a g t g a g t t t c c g c c a t t a t g c c a a t a g g t g t c t t a g t c c c c t a t t g c g t c c t

4680 a a g a a c a t g t g a g c a a a a g g c c a g c a a a a g g c c a g g a a c c g t a a a a a g g c c g c g t t g c t g t t c t t g t a c a c t c g t t t t c c g g t c g t t t t c c g g t c c t t g g c a t t t t t c c g g c g c a a c g a c

4740 ori g c g t t t t t c c a t a g g c t c c g c c c c c c t g a c g a g c a t c a c a a a a a t c g a c g c t c a a g t c a g c g c a a a a a g g t a t c c g a g g c g g g g g g a c t g c t c g t a g t g t t t t t a g c t g c g a g t t c a g t c

4800 ori T T T C T A T G G T C C G C A A A G G G pBR322ori-F a g g t g g c g a a a c c c g a c a g g a c t a t a a a g a t a c c a g g c g t t t c c c c c t g g a a g c t c c c t c t c c a c c g c t t t g g g c t g t c c t g a t a t t t c t a t g g t c c g c a a a g g g g g a c c t t c g a g g g a g

4860 ori g t g c g c t c t c c t g t t c c g a c c c t g c c g c t t a c c g g a t a c c t g t c c g c c t t t c t c c c t t c g c a c g c g a g a g g a c a a g g c t g g g a c g g c g a a t g g c c t a t g g a c a g g c g g a a a g a g g g a a g c

4920 ori g g a a g c g t g g c g c t t t c t c a t a g c t c a c g c t g t a g g t a t c t c a g t t c g g t g t a g g t c g t t c c t t c g c a c c g c g a a a g a g t a t c g a g t g c g a c a t c c a t a g a g t c a a g c c a c a t c c a g c a a

4980 ori c g c t c c a a g c t g g g c t g t g t g c a c g a a c c c c c c g t t c a g c c c g a c c g c t g c g c c t t a t c c g c g a g g t t c g a c c c g a c a c a c g t g c t t g g g g g g c a a g t c g g g c t g g c g a c g c g g a a t a g g

5040 ori g g t a a c t a t c g t c t t g a g t c c a a c c c g g t a a g a c a c g a c t t a t c g c c a c t g g c a g c a g c c c c a t t g a t a g c a g a a c t c a g g t t g g g c c a t t c t g t g c t g a a t a g c g g t g a c c g t c g t c g g

5100 ori a c t g g t a a c a g g a t t a g c a g a g c g a g g t a t g t a g g c g g t g c t a c a g a g t t c t t g a a g t g g t g a c c a t t g t c c t a a t c g t c t c g c t c c a t a c a t c c g c c a c g a t g t c t c a a g a a c t t c a c c

5160 ori t g g c c t a a c t a c g g c t a c a c t a g a a g a a c a g t a t t t g g t a t c t g c g c t c t g c t g a a g c c a a c c g g a t t g a t g c c g a t g t g a t c t t c t t g t c a t a a a c c a t a g a c g c g a g a c g a c t t c g g t

5220 ori g t t a c c t t c g g a a a a a g a g t t g g t a g c t c t t g a t c c g g c a a a c a a a c c a c c g c t g g t a g c c a a t g g a a g c c t t t t t c t c a a c c a t c g a g a a c t a g g c c g t t t g t t t g g t g g c g a c c a t c g

5280 ori g g t g g t t t t t t t g t t t g c a a g c a g c a g a t t a c g c g c a g a a a a a a a g g a t c t c a a g a a g a t c c a c c a a a a a a a c a a a c g t t c g t c g t c t a a t g c g c g t c t t t t t t t c c t a g a g t t c t t c t a

5340 c c t t t g a t c t t t t c t a c g g g g t c t g a c g c t c a g t g g a a c g a a a a c t c a c g t t a a g g g a t t g g a a a c t a g a a a a g a t g c c c c a g a c t g c g a g t c a c c t t g c t t t t g a g t g c a a t t c c c t a a

5400 t t g g t c a t g a g a t t a t c a a a a a g g a t c t t c a c c t a g a t c c t t t t a a a t t a a a a a t g a a g t a a c c a g t a c t c t a a t a g t t t t t c c t a g a a g t g g a t c t a g g a a a a t t t a a t t t t t a c t t c a

5460 285 *WHKI AmpR *WHKI t t t a a a t c a a t c t a a a g t a t a t a t g a g t a a a c t t g g t c t g a c a g t t a c c a a t g c t t a a t c a a a t t t a g t t a g a t t t c a t a t a t a c t c a t t t g a a c c a g a c t g t c a a t g g t t a c g a a t t a g

AhdI 5520 280 275 270 265 LSAGIEAIQRNREDMTAQSG AmpR LSAGIEAIQRNREDMTAQSG a g t g a g g c a c c t a t c t c a g c g a t c t g t c t a t t t c g t t c a t c c a t a g t t g c c t g a c t c c c c t c a c t c c g t g g a t a g a g t c g c t a g a c a g a t a a a g c a a g t a g g t a t c a a c g g a c t g a g g g g

5580 260 255 250 245 TTYIVVIRSPKGDPGLAAII AmpR TTYIVVIRSPKGDPGLAAII g t c g t g t a g a t a a c t a c g a t a c g g g a g g g c t t a c c a t c t g g c c c c a g t g c t g c a a t g a t a c a g c a c a t c t a t t g a t g c t a t g c c c t c c c g a a t g g t a g a c c g g g g t c a c g a c g t t a c t a t

5640 240 235 230 225 GRSGREGAGSKDAIFWGAPL AmpR GRSGREGAGSKDAIFWGAPL c c g c g a g a c c c a c g c t c a c c g g c t c c a g a t t t a t c a g c a a t a a a c c a g c c a g c c g g a a g g g g c g c t c t g g g t g c g a g t g g c c g a g g t c t a a a t a g t c g t t a t t t g g t c g g t c g g c c t t c c

5700 220 215 210 205 ASRLLPGAVKDAEMWDILQQ AmpR ASRLLPGAVKDAEMWDILQQ g c c g a g c g c a g a a g t g g t c c t g c a a c t t t a t c c g c c t c c a t c c a g t c t a t t a a t t g t t g c c g g c t c g c g t c t t c a c c a g g a c g t t g a a a t a g g c g g a g g t a g g t c a g a t a a t t a a c a a c g

5760 200 195 190 185 RSALTLLEGTLLKRLTTAMA AmpR RSALTLLEGTLLKRLTTAMA c g g g a a g c t a g a g t a a g t a g t t c g c c a g t t a a t a g t t t g c g c a a c g t t g t t g c c a t t g c t g c c c t t c g a t c t c a t t c a t c a a g c g g t c a a t t a t c a a a c g c g t t g c a a c a a c g g t a a c g a

5820 180 175 170 165 VPMTTDREDNPIAENLEPEW AmpR VPMTTDREDNPIAENLEPEW a c a g g c a t c g t g g t g t c a c g c t c g t c g t t t g g t a t g g c t t c a t t c a g c t c c g g t t c c c a a t g t c c g t a g c a c c a c a g t g c g a g c a g c a a a c c a t a c c g a a g t a a g t c g a g g c c a a g g g t t

5880 160 155 150 145 RDLRTVHDGMNHLFATLEKP AmpR RDLRTVHDGMNHLFATLEKP c g a t c a a g g c g a g t t a c a t g a t c c c c c a t g t t g t g c a a a a a a g c g g t t a g c t c c t t c g g t g c t a g t t c c g c t c a a t g t a c t a g g g g g t a c a a c a c g t t t t t t c g c c a a t c g a g g a a g c c a

PvuI 5940 140 135 130 125 GGITTLLLNAATNDSMTIAA AmpR GGITTLLLNAATNDSMTIAA c c t c c g a t c g t t g t c a g a a g t a a g t t g g c c g c a g t g t t a t c a c t c a t g g t t a t g g c a g c a g g a g g c t a g c a a c a g t c t t c a t t c a a c c g g c g t c a c a a t a g t g a g t a c c a a t a c c g t c g t

6000 120 115 110 105 SCLERVTMGDTLHKETVPSY AmpR SCLERVTMGDTLHKETVPSY c t g c a t a a t t c t c t t a c t g t c a t g c c a t c c g t a a g a t g c t t t t c t g t g a c t g g t g a g t a c g a c g t a t t a a g a g a a t g a c a g t a c g g t a g g c a t t c t a c g a a a a g a c a c t g a c c a c t c a t g

6060 100 95 90 85 EVLDNQSYHIRRGLQEQGAD AmpR EVLDNQSYHIRRGLQEQGAD t c a a c c a a g t c a t t c t g a g a a t a g t g t a t g c g g c g a c c g a g t t g c t c t t g c c c g g c g t c a a g t t g g t t c a g t a a g a c t c t t a t c a c a t a c g c c g c t g g c t c a a c g a g a a c g g g c c g c a g t

6120 80 75 70 65 IRSLVAGCLLVKFTSMMPFR AmpR A T A A T A C C G C G C C A C A T A G C Amp-R IRSLVAGCLLVKFTSMMPFR a t a c g g g a t a a t a c c g c g c c a c a t a g c a g a a c t t t a a a a g t g c t c a t c a t t g g a a a a c g t t a t g c c c t a t t a t g g c g c g g t g t a t c g t c t t g a a a t t t t c a c g a g t a g t a a c c t t t t g c a

6180 60 55 50 45 EEPRFSELIKGSNLDLEIYG AmpR EEPRFSELIKGSNLDLEIYG t c t t c g g g g c g a a a a c t c t c a a g g a t c t t a c c g c t g t t g a g a t c c a g t t c g a t g t a a c c c a g a a g c c c c g c t t t t g a g a g t t c c t a g a a t g g c g a c a a c t c t a g g t c a a g c t a c a t t g g g

6240 40 35 30 25 VRAGLQDEADKVKVLTEPH signa... A AmpR VRAGLQDEADKVKVLTEPHA a c t c g t g c a c c c a a c t g a t c t t c a g c a t c t t t t a c t t t c a c c a g c g t t t c t g g g t g a g c a t g a g c a c g t g g g t t g a c t a g a a g t c g t a g a a a a t g a a a g t g g t c g c a a a g a c c c a c t c g t

6300 20 15 10 5 signal sequence FVPLCFAAFFPILAVRFHQI AmpR FVPLCFAAFFPILAVRFHQI a a a a c a g g a a g g c a a a a t g c c g c a a a a a a g g g a a t a a g g g c g a c a c g g a a a t g t t g a a t a t t t t g t c c t t c c g t t t t a c g g c g t t t t t t c c c t t a t t c c c g c t g t g c c t t t a c a a c t t a t

SspI 6360 1 signal... SM AmpR AmpR promoter SM c t c a t a c t c t t c c t t t t t c a a t a t t a t t g a a g c a t t t a t c a g g g t t a t t g t c t c a t g a g c g a g t a t g a g a a g g a a a a a g t t a t a a t a a c t t c g t a a a t a g t c c c a a t a a c a g a g t a c t c g

6420 AmpR promoter g g a t a c a t a t t t g a a t g t a t t t a g a a a a a t a a a c a a a t a g g g g t t c c g c g c a c a t t t c c c c c t a t g t a t a a a c t t a c a t a a a t c t t t t t a t t t g t t t a t c c c c a a g g c g c g t g t a a a g g g

BglIISgrDI 6480 c g a a a a g t g c c a c c t g a c g t c g a c g g a t c g g g a g a t c t c c c g a t c c c c t a t g g t g c a c t c g c t t t t c a c g g t g g a c t g c a g c t g c c t a g c c c t c t a g a g g g c t a g g g g a t a c c a c g t g a g

6540 A T G T T A G A C G A G A C T A C G G C pRS-marker t c a g t a c a a t c t g c t c t g a t g c c g c a t a g t t a a g c c a g t a t c t g c t c c c t g c t t g t g t g t a g t c a t g t t a g a c g a g a c t a c g g c g t a t c a a t t c g g t c a t a g a c g a g g g a c g a a c a c a c a

6600 t g g a g g t c g c t g a g t a g t g c g c g a g c a a a a t t t a a g c t a c a a c a a g g c a a g g c t t g a c c g a c c t c c a g c g a c t c a t c a c g c g c t c g t t t t a a a t t c g a t g t t g t t c c g t t c c g a a c t g g c

3ʹ 5ʹ 6602 a c t g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.