Addgene pET30a-ASXL1-HARE-HTH domain Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: pET30a-ASXL1-HARE-HTH domain Sequencing Result


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T7 Term (5529 .. 5547) BlpI (5532) 6xHis PaeR7I - PspXI - XhoI (5453) PstI (5283) BfuAI - BspMI (5272) PmlI (5211) BglII (5173) BstBI (5148) thrombin site 6xHis NdeI (5070) RBS XbaI (5030) T7 promoter T7 (4984 .. 5003) SgrAI (4919) pBRrevBam (4877 .. 4896) SphI (4771) BstAPI (4562) LacI-R (4555 .. 4574) MluI (4238) BclI * (4224) BstEII (4056) ApaI (4035) PspOMI (4031) HincII - HpaI (3736) PshAI (3397) BglI (3181) FspI - FspAI (3160) PpuMI (3132) StyI (5554) F1ori-R (99 .. 118) DraIII (245) F1ori-F (309 .. 330) AsiSI - PvuI (944) TspMI - XmaI (1066) SmaI (1068) Kan-R (1282 .. 1301) NruI (1285) pENTR-R (1366 .. 1385) BssSI - BssSαI (1967) pBR322ori-F (1985 .. 2004) PciI (2140) L4440 (2238 .. 2255) BspQI - SapI (2257) TatI (2333) pRS-marker (2335 .. 2354) AccI (2369) BstZ17I (2370) PflFI - Tth111I (2395) pGEX 3' (2495 .. 2517) pET30a-ASXL1-HARE-HTH domain 5615 bp

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5ʹ 3ʹ 60 f1 ori t g g c g a a t g g g a c g c g c c c t g t a g c g g c g c a t t a a g c g c g g c g g g t g t g g t g g t t a c g c g a c c g c t t a c c c t g c g c g g g a c a t c g c c g c g t a a t t c g c g c c g c c c a c a c c a c c a a t g c g c

120 f1 ori G A G G A A A G C G A A A G A A G G G A F1ori-R c a g c g t g a c c g c t a c a c t t g c c a g c g c c c t a g c g c c c g c t c c t t t c g c t t t c t t c c c t t c g t c g c a c t g g c g a t g t g a a c g g t c g c g g g a t c g c g g g c g a g g a a a g c g a a a g a a g g g a a g

180 f1 ori c t t t c t c g c c a c g t t c g c c g g c t t t c c c c g t c a a g c t c t a a a t c g g g g g c t c c c t t t a g g g a a a g a g c g g t g c a a g c g g c c g a a a g g g g c a g t t c g a g a t t t a g c c c c c g a g g g a a a t c c

240 f1 ori g t t c c g a t t t a g t g c t t t a c g g c a c c t c g a c c c c a a a a a a c t t g a t t a g g g t g a t g g t t c c a a g g c t a a a t c a c g a a a t g c c g t g g a g c t g g g g t t t t t t g a a c t a a t c c c a c t a c c a a g

DraIII 300 f1 ori a c g t a g t g g g c c a t c g c c c t g a t a g a c g g t t t t t c g c c c t t t g a c g t t g g a g t c c a c g t t t g c a t c a c c c g g t a g c g g g a c t a t c t g c c a a a a a g c g g g a a a c t g c a a c c t c a g g t g c a a

360 f1 ori G T G G A C T C T T G T T C C A A A C T G G F1ori-F c t t t a a t a g t g g a c t c t t g t t c c a a a c t g g a a c a a c a c t c a a c c c t a t c t c g g t c t a t t c g a a a t t a t c a c c t g a g a a c a a g g t t t g a c c t t g t t g t g a g t t g g g a t a g a g c c a g a t a a g

420 f1 ori t t t t g a t t t a t a a g g g a t t t t g c c g a t t t c g g c c t a t t g g t t a a a a a a t g a g c t g a t t t a a a a a c t a a a t a t t c c c t a a a a c g g c t a a a g c c g g a t a a c c a a t t t t t t a c t c g a c t a a a t

480 f1 ori a c a a a a a t t t a a c g c g a a t t t t a a c a a a a t a t t a a c g c t t a c a a t t t a g g t g g c a c t t t t t g t t t t t a a a t t g c g c t t a a a a t t g t t t t a t a a t t g c g a a t g t t a a a t c c a c c g t g a a a a

540 c g g g g a a a t g t g c g c g g a a c c c c t a t t t g t t t a t t t t t c t a a a t a c a t t c a a a t a t g t a t g c c c c t t t a c a c g c g c c t t g g g g a t a a a c a a a t a a a a a g a t t t a t g t a a g t t t a t a c a t a

600 270 265 260 *FFEDLMLHFQLKN KanR 270 265 260 *FFEDLMLHFQLKN KanR *FFEDLMLHFQLKN c c g c t c a t g a a t t a a t t c t t a g a a a a a c t c a t c g a g c a t c a a a t g a a a c t g c a a t t t a t t g g c g a g t a c t t a a t t a a g a a t c t t t t t g a g t a g c t c g t a g t t t a c t t t g a c g t t a a a t a a

660 255 250 245 240 MDPNDIGYKQFLRKQLSPSF KanR 255 250 245 240 MDPNDIGYKQFLRKQLSPSF KanR MDPNDIGYKQFLRKQLSPSF c a t a t c a g g a t t a t c a a t a c c a t a t t t t t g a a a a a g c c g t t t c t g t a a t g a a g g a g a a a a g t a t a g t c c t a a t a g t t a t g g t a t a a a a a c t t t t t c g g c a a a g a c a t t a c t t c c t c t t t t

720 235 230 225 220 EGLCNWLIALDQYRDAIGVR KanR 235 230 225 220 EGLCNWLIALDQYRDAIGVR KanR EGLCNWLIALDQYRDAIGVR c t c a c c g a g g c a g t t c c a t a g g a t g g c a a g a t c c t g g t a t c g g t c t g c g a t t c c g a c t c g g a g t g g c t c c g t c a a g g t a t c c t a c c g t t c t a g g a c c a t a g c c a g a c g c t a a g g c t g a g c

780 215 210 205 200 GVDICGILKGEDFILNDLSF KanR 215 210 205 200 GVDICGILKGEDFILNDLSF KanR GVDICGILKGEDFILNDLSF t c c a a c a t c a a t a c a a c c t a t t a a t t t c c c c t c g t c a a a a a t a a g g t t a t c a a g t g a g a a a g g t t g t a g t t a t g t t g g a t a a t t a a a g g g g a g c a g t t t t t a t t c c a a t a g t t c a c t c t t

840 195 190 185 180 DGHTVVSDPSFPLLKHMEKW KanR 195 190 185 180 DGHTVVSDPSFPLLKHMEKW KanR DGHTVVSDPSFPLLKHMEKW a t c a c c a t g a g t g a c g a c t g a a t c c g g t g a g a a t g g c a a a a g t t t a t g c a t t t c t t t c c a t a g t g g t a c t c a c t g c t g a c t t a g g c c a c t c t t a c c g t t t t c a a a t a c g t a a a g a a a g g t

900 175 170 165 160 VQEVPWGNREDDFDSADVLG KanR 175 170 165 160 VQEVPWGNREDDFDSADVLG KanR VQEVPWGNREDDFDSADVLG g a c t t g t t c a a c a g g c c a g c c a t t a c g c t c g t c a t c a a a a t c a c t c g c a t c a a c c a a a c c c t g a a c a a g t t g t c c g g t c g g t a a t g c g a g c a g t a g t t t t a g t g a g c g t a g t t g g t t t g g

AsiSIPvuI 960 155 150 145 140 NNMRSQAQALRFVRDSNFPC KanR 155 150 145 140 NNMRSQAQALRFVRDSNFPC KanR NNMRSQAQALRFVRDSNFPC g t t a t t c a t t c g t g a t t g c g c c t g a g c g a g a c g a a a t a c g c g a t c g c t g t t a a a a g g a c a c a a t a a g t a a g c a c t a a c g c g g a c t c g c t c t g c t t t a t g c g c t a g c g a c a a t t t t c c t g t

1020 135 130 125 120 NCVPISHLRRLFVALADVIN KanR 135 130 125 120 NCVPISHLRRLFVALADVIN KanR NCVPISHLRRLFVALADVIN a t t a c a a a c a g g a a t c g a a t g c a a c c g g c g c a g g a a c a c t g c c a g c g c a t c a a c a a t a t t t a a t g t t t g t c c t t a g c t t a c g t t g g c c g c g t c c t t g t g a c g g t c g c g t a g t t g t t a t a a

TspMIXmaISmaI 1080 115 110 105 100 EGSDPYEELVQFATKGPIAT KanR 115 110 105 100 EGSDPYEELVQFATKGPIAT KanR EGSDPYEELVQFATKGPIAT t t c a c c t g a a t c a g g a t a t t c t t c t a a t a c c t g g a a t g c t g t t t t c c c g g g g a t c g c a g t a a g t g g a c t t a g t c c t a t a a g a a g a t t a t g g a c c t t a c g a c a a a a g g g c c c c t a g c g t c a

1140 95 90 85 80 TLLWADDPTRIFHKITPLPM KanR 95 90 85 80 TLLWADDPTRIFHKITPLPM KanR TLLWADDPTRIFHKITPLPM g g t g a g t a a c c a t g c a t c a t c a g g a g t a c g g a t a a a a t g c t t g a t g g t c g g a a g a g g c a t c c a c t c a t t g g t a c g t a g t a g t c c t c a t g c c t a t t t t a c g a a c t a c c a g c c t t c t c c g t a

1200 75 70 65 60 FETLWNLRVMEDTVDNAVSG KanR 75 70 65 60 FETLWNLRVMEDTVDNAVSG KanR FETLWNLRVMEDTVDNAVSG a a a t t c c g t c a g c c a g t t t a g t c t g a c c a t c t c a t c t g t a a c a t c a t t g g c a a c g c t a c c t t t a a g g c a g t c g g t c a a a t c a g a c t g g t a g a g t a g a c a t t g t a g t a a c c g t t g c g a t g g

1260 55 50 45 40 KGHKLFLEPADPKGYLRYIT KanR 55 50 45 40 KGHKLFLEPADPKGYLRYIT KanR KGHKLFLEPADPKGYLRYIT t t t g c c a t g t t t c a g a a a c a a c t c t g g c g c a t c g g g c t t c c c a t a c a a t c g a t a g a t t g t a a a c g g t a c a a a g t c t t t g t t g a g a c c g c g t a g c c c g a a g g g t a t g t t a g c t a t c t a a c a

NruI 1320 35 30 25 20 AGSQGVNDRAWKYGYLDADM KanR 35 30 25 20 AGSQGVNDRAWKYGYLDADM KanR A T C G C G A G C C C A T T T A T A C C Kan-R AGSQGVNDRAWKYGYLDADM c g c a c c t g a t t g c c c g a c a t t a t c g c g a g c c c a t t t a t a c c c a t a t a a a t c a g c a t c c a t g c g t g g a c t a a c g g g c t g t a a t a g c g c t c g g g t a a a t a t g g g t a t a t t t a g t c g t a g g t a

1380 15 10 5 1 NSNLRPRSCSTERQIHSM KanR 15 10 5 1 NSNLRPRSCSTERQIHSM KanR A T G G C T C A T A A C A C C pENTR-R NSNLRPRSCSTERQIHSM g t t g g a a t t t a a t c g c g g c c t a g a g c a a g a c g t t t c c c g t t g a a t a t g g c t c a t a a c a c c c a a c c t t a a a t t a g c g c c g g a t c t c g t t c t g c a a a g g g c a a c t t a t a c c g a g t a t t g t g g

1440 C C T T G pENTR-R c c t t g t a t t a c t g t t t a t g t a a g c a g a c a g t t t t a t t g t t c a t g a c c a a a a t c c c t t a a c g g a a c a t a a t g a c a a a t a c a t t c g t c t g t c a a a a t a a c a a g t a c t g g t t t t a g g g a a t t g

1500 ori g t g a g t t t t c g t t c c a c t g a g c g t c a g a c c c c g t a g a a a a g a t c a a a g g a t c t t c t t g a g c a c t c a a a a g c a a g g t g a c t c g c a g t c t g g g g c a t c t t t t c t a g t t t c c t a g a a g a a c t c

1560 ori a t c c t t t t t t t c t g c g c g t a a t c t g c t g c t t g c a a a c a a a a a a a c c a c c g c t a c c a g c g g t a g g a a a a a a a g a c g c g c a t t a g a c g a c g a a c g t t t g t t t t t t t g g t g g c g a t g g t c g c c

1620 ori t g g t t t g t t t g c c g g a t c a a g a g c t a c c a a c t c t t t t t c c g a a g g t a a c t g g c t t c a g c a a c c a a a c a a a c g g c c t a g t t c t c g a t g g t t g a g a a a a a g g c t t c c a t t g a c c g a a g t c g t

1680 ori g a g c g c a g a t a c c a a a t a c t g t c c t t c t a g t g t a g c c g t a g t t a g g c c a c c a c t t c a a g a c t c g c g t c t a t g g t t t a t g a c a g g a a g a t c a c a t c g g c a t c a a t c c g g t g g t g a a g t t c t

1740 ori a c t c t g t a g c a c c g c c t a c a t a c c t c g c t c t g c t a a t c c t g t t a c c a g t g g c t g c t g c c a t g a g a c a t c g t g g c g g a t g t a t g g a g c g a g a c g a t t a g g a c a a t g g t c a c c g a c g a c g g t

1800 ori g t g g c g a t a a g t c g t g t c t t a c c g g g t t g g a c t c a a g a c g a t a g t t a c c g g a t a a g g c g c c a c c g c t a t t c a g c a c a g a a t g g c c c a a c c t g a g t t c t g c t a t c a a t g g c c t a t t c c g c g

1860 ori a g c g g t c g g g c t g a a c g g g g g g t t c g t g c a c a c a g c c c a g c t t g g a g c g a a c g a c c t a c a t c g c c a g c c c g a c t t g c c c c c c a a g c a c g t g t g t c g g g t c g a a c c t c g c t t g c t g g a t g t

1920 ori c c g a a c t g a g a t a c c t a c a g c g t g a g c t a t g a g a a a g c g c c a c g c t t c c c g a a g g g a g a a g g c t t g a c t c t a t g g a t g t c g c a c t c g a t a c t c t t t c g c g g t g c g a a g g g c t t c c c t c t t

BssSαIBssSI 1980 ori a g g c g g a c a g g t a t c c g g t a a g c g g c a g g g t c g g a a c a g g a g a g c g c a c g a g g g a g c t t c t c c g c c t g t c c a t a g g c c a t t c g c c g t c c c a g c c t t g t c c t c t c g c g t g c t c c c t c g a a g

2040 ori G G G A A A C G C C T G G T A T C T T T pBR322ori-F c a g g g g g a a a c g c c t g g t a t c t t t a t a g t c c t g t c g g g t t t c g c c a c c t c t g a c t t g a g c g t c c c c c t t t g c g g a c c a t a g a a a t a t c a g g a c a g c c c a a a g c g g t g g a g a c t g a a c t c g

2100 ori g t c g a t t t t t g t g a t g c t c g t c a g g g g g g c g g a g c c t a t g g a a a a a c g c c a g c a a c g c g g c a g c t a a a a a c a c t a c g a g c a g t c c c c c c g c c t c g g a t a c c t t t t t g c g g t c g t t g c g c c

PciI 2160 c c t t t t t a c g g t t c c t g g c c t t t t g c t g g c c t t t t g c t c a c a t g t t c t t t c c t g c g t t a t g g a a a a a t g c c a a g g a c c g g a a a a c g a c c g g a a a a c g a g t g t a c a a g a a a g g a c g c a a t a

2220 c c c c t g a t t c t g t g g a t a a c c g t a t t a c c g c c t t t g a g t g a g c t g a t a c c g c t c g c c g c a g g g g a c t a a g a c a c c t a t t g g c a t a a t g g c g g a a a c t c a c t c g a c t a t g g c g a g c g g c g t

BspQISapI 2280 bom A G C G A G T C A G T G A G C G A G L4440 g c c g a a c g a c c g a g c g c a g c g a g t c a g t g a g c g a g g a a g c g g a a g a g c g c c t g a t g c g g t c g g c t t g c t g g c t c g c g t c g c t c a g t c a c t c g c t c c t t c g c c t t c t c g c g g a c t a c g c c a

TatI 2340 bom A T G T T A pRS-marker a t t t t c t c c t t a c g c a t c t g t g c g g t a t t t c a c a c c g c a a t g g t g c a c t c t c a g t a c a a t t a a a a g a g g a a t g c g t a g a c a c g c c a t a a a g t g t g g c g t t a c c a c g t g a g a g t c a t g t t a

Tth111IPflFIAccIBstZ17I 2400 bom G A C G A G A C T A C G G C pRS-marker c t g c t c t g a t g c c g c a t a g t t a a g c c a g t a t a c a c t c c g c t a t c g c t a c g t g a c t g g g t c g a c g a g a c t a c g g c g t a t c a a t t c g g t c a t a t g t g a g g c g a t a g c g a t g c a c t g a c c c a g

2460 bom a t g g c t g c g c c c c g a c a c c c g c c a a c a c c c g c t g a c g c g c c c t g a c g g g c t t g t c t g c t c t a c c g a c g c g g g g c t g t g g g c g g t t g t g g g c g a c t g c g c g g g a c t g c c c g a a c a g a c g a g

2520 *LN rop C C G G G A G C T G C A T G T G T C A G A G G pGEX 3' c c g g c a t c c g c t t a c a g a c a a g c t g t g a c c g t c t c c g g g a g c t g c a t g t g t c a g a g g t t t g g c c g t a g g c g a a t g t c t g t t c g a c a c t g g c a g a g g c c c t c g a c g t a c a c a g t c t c c a a a

2580 60 55 50 45 EGDDGFRALCSRYLEDAHDH rop t c a c c g t c a t c a c c g a a a c g c g c g a g g c a g c t g c g g t a a a g c t c a t c a g c g t g g t c g t g a a g t g g c a g t a g t g g c t t t g c g c g c t c c g t c g a c g c c a t t t c g a g t a g t c g c a c c a g c a c t

2640 40 35 30 25 LSECIDAQEDADLENLKELL rop a g c g a t t c a c a g a t g t c t g c c t g t t c a t c c g c g t c c a g c t c g t t g a g t t t c t c c a g a a g c t c g c t a a g t g t c t a c a g a c g g a c a a g t a g g c g c a g g t c g a g c a a c t c a a a g a g g t c t t c g

2700 20 15 10 5 TLTQSRIFRAMNLATKEQKT rop g t t a a t g t c t g g c t t c t g a t a a a g c g g g c c a t g t t a a g g g c g g t t t t t t c c t g t t t g g t c c a a t t a c a g a c c g a a g a c t a t t t c g c c c g g t a c a a t t c c c g c c a a a a a a g g a c a a a c c a g

2760 1 M rop a c t g a t g c c t c c g t g t a a g g g g g a t t t c t g t t c a t g g g g g t a a t g a t a c c g a t g a a a c g a t g a c t a c g g a g g c a c a t t c c c c c t a a a g a c a a g t a c c c c c a t t a c t a t g g c t a c t t t g c t

2820 g a g a g g a t g c t c a c g a t a c g g g t t a c t g a t g a t g a a c a t g c c c g g t t a c t g g a a c g t t g t c t c t c c t a c g a g t g c t a t g c c c a a t g a c t a c t a c t t g t a c g g g c c a a t g a c c t t g c a a c a

2880 g a g g g t a a a c a a c t g g c g g t a t g g a t g c g g c g g g a c c a g a g a a a a a t c a c t c a g g g t c a a c t c c c a t t t g t t g a c c g c c a t a c c t a c g c c g c c c t g g t c t c t t t t t a g t g a g t c c c a g t t

2940 t g c c a g c g c t t c g t t a a t a c a g a t g t a g g t g t t c c a c a g g g t a g c c a g c a g c a t c c t g c g a c g g t c g c g a a g c a a t t a t g t c t a c a t c c a c a a g g t g t c c c a t c g g t c g t c g t a g g a c g c

3000 a t g c a g a t c c g g a a c a t a a t g g t g c a g g g c g c t g a c t t c c g c g t t t c c a g a c t t t a c g a a t a c g t c t a g g c c t t g t a t t a c c a c g t c c c g c g a c t g a a g g c g c a a a g g t c t g a a a t g c t t

3060 a c a c g g a a a c c g a a g a c c a t t c a t g t t g t t g c t c a g g t c g c a g a c g t t t t g c a g c a g c a g t g t g c c t t t g g c t t c t g g t a a g t a c a a c a a c g a g t c c a g c g t c t g c a a a a c g t c g t c g t c

3120 t c g c t t c a c g t t c g c t c g c g t a t c g g t g a t t c a t t c t g c t a a c c a g t a a g g c a a c c c c g c a g c g a a g t g c a a g c g a g c g c a t a g c c a c t a a g t a a g a c g a t t g g t c a t t c c g t t g g g g c g

FspAIFspIPpuMI 3180 c a g c c t a g c c g g g t c c t c a a c g a c a g g a g c a c g a t c a t g c g c a c c c g t g g g g c c g c c a t g g t c g g a t c g g c c c a g g a g t t g c t g t c c t c g t g c t a g t a c g c g t g g g c a c c c c g g c g g t a c

BglI 3240 c c g g c g a t a a t g g c c t g c t t c t c g c c g a a a c g t t t g g t g g c g g g a c c a g t g a c g a a g g c t g g c c g c t a t t a c c g g a c g a a g a g c g g c t t t g c a a a c c a c c g c c c t g g t c a c t g c t t c c g a

3300 t g a g c g a g g g c g t g c a a g a t t c c g a a t a c c g c a a g c g a c a g g c c g a t c a t c g t c g c g c t c a c t c g c t c c c g c a c g t t c t a a g g c t t a t g g c g t t c g c t g t c c g g c t a g t a g c a g c g c g a g

3360 c a g c g a a a g c g g t c c t c g c c g a a a a t g a c c c a g a g c g c t g c c g g c a c c t g t c c t a c g a g t g t c g c t t t c g c c a g g a g c g g c t t t t a c t g g g t c t c g c g a c g g c c g t g g a c a g g a t g c t c a

PshAI 3420 t g c a t g a t a a a g a a g a c a g t c a t a a g t g c g g c g a c g a t a g t c a t g c c c c g c g c c c a c c g g a c g t a c t a t t t c t t c t g t c a g t a t t c a c g c c g c t g c t a t c a g t a c g g g g c g c g g g t g g c c

3480 a a g g a g c t g a c t g g g t t g a a g g c t c t c a a g g g c a t c g g t c g a g a t c c c g g t g c c t a a t g a t t c c t c g a c t g a c c c a a c t t c c g a g a g t t c c c g t a g c c a g c t c t a g g g c c a c g g a t t a c t

3540 360 355 *QGSELRSVQ lacI *QGSELRSVQ g t g a g c t a a c t t a c a t t a a t t g c g t t g c g c t c a c t g c c c g c t t t c c a g t c g g g a a a c c t g c a c t c g a t t g a a t g t a a t t a a c g c a a c g c g a g t g a c g g g c g a a a g g t c a g c c c t t t g g a c

3600 350 345 340 335 RALQMLSDALARPSATQTNP lacI RALQMLSDALARPSATQTNP t c g t g c c a g c t g c a t t a a t g a a t c g g c c a a c g c g c g g g g a g a g g c g g t t t g c g t a t t g g g a g c a c g g t c g a c g t a a t t a c t t a g c c g g t t g c g c g c c c c t c t c c g c c a a a c g c a t a a c c c

3660 330 325 320 315 ALTTKRKVLSVPLLQNGKVA lacI ALTTKRKVLSVPLLQNGKVA c g c c a g g g t g g t t t t t c t t t t c a c c a g t g a g a c g g g c a a c a g c t g a t t g c c c t t c a c c g c g c g g t c c c a c c a a a a a g a a a a g t g g t c a c t c t g c c c g t t g t c g a c t a a c g g g a a g t g g c g

3720 310 305 300 295 QGQSLQLLRDVSTQGLLRFD lacI QGQSLQLLRDVSTQGLLRFD c t g g c c c t g a g a g a g t t g c a g c a a g c g g t c c a c g c t g g t t t g c c c c a g c a g g c g a a a a t c g a c c g g g a c t c t c t c a a c g t c g t t c g c c a g g t g c g a c c a a a c g g g g t c g t c c g c t t t t a g

HincIIHpaI 3780 290 285 280 275 QKITTLPPIYCSSDETDDYG lacI QKITTLPPIYCSSDETDDYG c t g t t t g a t g g t g g t t a a c g g c g g g a t a t a a c a t g a g c t g t c t t c g g t a t c g t c g t a t c c g a c a a a c t a c c a c c a a t t g c c g c c c t a t a t t g t a c t c g a c a g a a g c c a t a g c a g c a t a g g

3840 270 265 260 255 VVSIDAGVRLGSETIARMAG lacI VVSIDAGVRLGSETIARMAG c a c t a c c g a g a t g t c c g c a c c a a c g c g c a g c c c g g a c t c g g t a a t g g c g c g c a t t g c g c c g t g a t g g c t c t a c a g g c g t g g t t g c g c g t c g g g c c t g a g c c a t t a c c g c g c g t a a c g c g g

3900 250 245 240 235 LAMQDNAVLMATPVIGENLM lacI LAMQDNAVLMATPVIGENLM c a g c g c c a t c t g a t c g t t g g c a a c c a g c a t c g c a g t g g g a a c g a t g c c c t c a t t c a g c a t g t c g c g g t a g a c t a g c a a c c g t t g g t c g t a g c g t c a c c c t t g c t a c g g g a g t a a g t c g t a

3960 230 225 220 215 QMTQQFGSMASWDGEREAIP lacI QMTQQFGSMASWDGEREAIP t t g c a t g g t t t g t t g a a a a c c g g a c a t g g c a c t c c a g t c g c c t t c c c g t t c c g c t a t c g g a a c g t a c c a a a c a a c t t t t g g c c t g t a c c g t g a g g t c a g c g g a a g g g c a a g g c g a t a g c c

4020 210 205 200 195 QIQNRTLYKHWGALRLRASV lacI QIQNRTLYKHWGALRLRASV c t g a a t t t g a t t g c g a g t g a g a t a t t t a t g c c a g c c a g c c a g a c g c a g a c g c g c c g a g a c g a c t t a a a c t a a c g c t c a c t c t a t a a a t a c g g t c g g t c g g t c t g c g t c t g c g c g g c t c t g

BstEIIPspOMIApaI 4080 190 185 180 175 SSLPGALLAIQQHGLAVLHE lacI SSLPGALLAIQQHGLAVLHE a g a a c t t a a t g g g c c c g c t a a c a g c g c g a t t t g c t g g t g a c c c a a t g c g a c c a g a t g c t c t c t t g a a t t a c c c g g g c g a t t g t c g c g c t a a a c g a c c a c t g g g t t a c g c t g g t c t a c g a g

4140 170 165 160 155 VGLRTGDEHSFIISNIPTQD lacI VGLRTGDEHSFIISNIPTQD c a c g c c c a g t c g c g t a c c g t c t t c a t g g g a g a a a a t a a t a c t g t t g a t g g g t g t c t g g t c g t g c g g g t c a g c g c a t g g c a g a a g t a c c c t c t t t t a t t a t g a c a a c t a c c c a c a g a c c a g

4200 150 145 140 135 SVDLFLAPVNTCAAEVAIAD lacI SVDLFLAPVNTCAAEVAIAD a g a g a c a t c a a g a a a t a a c g c c g g a a c a t t a g t g c a g g c a g c t t c c a c a g c a a t g g c a t c t c t c t g t a g t t c t t t a t t g c g g c c t t g t a a t c a c g t c c g t c g a a g g t g t c g t t a c c g t a g

MluIBclI* 4260 130 125 120 115 QDDLPYNIILGSVRQALLNH lacI QDDLPYNIILGSVRQALLNH c t g g t c a t c c a g c g g a t a g t t a a t g a t c a g c c c a c t g a c g c g t t g c g c g a g a a g a t t g t g g a c c a g t a g g t c g c c t a t c a a t t a c t a g t c g g g t g a c t g c g c a a c g c g c t c t t c t a a c a c

4320 110 105 100 95 VAAKCAEVGSREVMSVVVSA lacI VAAKCAEVGSREVMSVVVSA c a c c g c c g c t t t a c a g g c t t c g a c g c c g c t t c g t t c t a c c a t c g a c a c c a c c a c g c t g g c g t g g c g g c g a a a t g t c c g a a g c t g c g g c g a a g c a a g a t g g t a g c t g t g g t g g t g c g a c c g

4380 90 85 80 75 GLQDARSKIAAVIQSPAHLA lacI GLQDARSKIAAVIQSPAHLA *DGRCNAVARAPG a c c c a g t t g a t c g g c g c g a g a t t t a a t c g c c g c g a c a a t t t g c g a c g g c g c g t g c a g g g c t g g g t c a a c t a g c c g c g c t c t a a a t t a g c g g c g c t g t t a a a c g c t g c c g c g c a c g t c c c g

4440 70 65 60 55 LSSTAVGILLSQKGALQQAV lacI LSSTAVGILLSQKGALQQAV SQLHCRWDAVVTQGGTTTGR c a g a c t g g a g g t g g c a a c g c c a a t c a g c a a c g a c t g t t t g c c c g c c a g t t g t t g t g c c a c g t c t g a c c t c c a c c g t t g c g g t t a g t c g t t g c t g a c a a a c g g g c g g t c a a c a a c a c g g t g

4500 50 45 40 35 RNPIYNLEAMAAEVKERTKA lacI RNPIYNLEAM PQSHLEAGGDGSGSKGANEC g c g g t t g g g a a t g t a a t t c a g c t c c g c c a t c g c c g c t t c c a c t t t t t c c c g c g t t t t c g c c g c c a a c c c t t a c a t t a a g t c g a g g c g g t a g c g g c g a a g g t g a a a a a g g g c g c a a a a g c g

4560 30 25 20 15 SVHSAQNVVRSVTQYSVGAY lacI G G C A T A LacI-R FRPQGPEGRPFRDSLLCRCV a g a a a c g t g g c t g g c c t g g t t c a c c a c g c g g g a a a c g g t c t g a t a a g a g a c a c c g g c a t a t c t t t g c a c c g a c c g g a c c a a g t g g t g c g c c c t t t g c c a g a c t a t t c t c t g t g g c c g t a t

BstAPI 4620 10 5 1 EAVDYLTVPKM lacI lacI promoter C T C T G C G A C A T C G T LacI-R RRCRIVNSTECEGGQISERG c t c t g c g a c a t c g t a t a a c g t t a c t g g t t t c a c a t t c a c c a c c c t g a a t t g a c t c t c t t c g a g a c g c t g t a g c a t a t t g c a a t g a c c a a a g t g t a a g t g g t g g g a c t t a a c t g a g a g a a g

4680 lacI promoter PAIMGYRSLNQAMRHHGPDR c g g g c g c t a t c a t g c c a t a c c g c g a a a g g t t t t g c g c c a t t c g a t g g t g t c c g g g a t c t c g c c c g c g a t a g t a c g g t a t g g c g c t t t c c a a a a c g c g g t a a g c t a c c a c a g g c c c t a g a g

4740 REGKHSEQMLFCGLLLNLGN g a c g c t c t c c c t t a t g c g a c t c c t g c a t t a g g a a g c a g c c c a g t a g t a g g t t g a g g c c g t c t g c g a g a g g g a a t a c g c t g a g g a c g t a a t c c t t c g t c g g g t c a t c a t c c a a c t c c g g c a

SphI 4800 LVAAALFPAHLSIAGLLGGA t g a g c a c c g c c g c c g c a a g g a a t g g t g c a t g c a a g g a g a t g g c g c c c a a c a g t c c c c c g g a c t c g t g g c g g c g g c g t t c c t t a c c a c g t a c g t t c c t c t a c c g c g g g t t g t c a g g g g g c c

4860 VPGAVMGVGFCASMLGFHRA c c a c g g g g c c t g c c a c c a t a c c c a c g c c g a a a c a a g c g c t c a t g a g c c c g a a g t g g c g a g g g t g c c c c g g a c g g t g g t a t g g g t g c g g c t t t g t t c g c g a g t a c t c g g g c t t c a c c g c t c

SgrAI 4920 G G T G A T G T C G G C G A T A T A G G pBRrevBam RDEGDTIDAIYAGAVAGTAG c c c g a t c t t c c c c a t c g g t g a t g t c g g c g a t a t a g g c g c c a g c a a c c g c a c c t g t g g c g c g g g c t a g a a g g g g t a g c c a c t a c a g c c g c t a t a t c c g c g g t c g t t g g c g t g g a c a c c g c g

4980 TIGAVIRGAYLISISRSGAF c g g t g a t g c c g g c c a c g a t g c g t c c g g c g t a g a g g a t c g a g a t c g a t c t c g a t c c c g c g a g c c a c t a c g g c c g g t g c t a c g c a g g c c g c a t c t c c t a g c t c t a g c t a g a g c t a g g g c g c t

XbaI 5040 T7 promoter lac operator T A A T A C G A C T C A C T A T A G G G T7 NIRSVIPSNHASLLEGRSII a a t t a a t a c g a c t c a c t a t a g g g g a a t t g t g a g c g g a t a a c a a t t c c c c t c t a g a a a t a a t t a a t t a t g c t g a g t g a t a t c c c c t t a a c a c t c g c c t a t t g t t a a g g g g a g a t c t t t a t t

NdeI 5100 1 5 HHHHHH SSG RBS 6xHis KNLKLFSIYMHVMMMMM t t t t g t t t a a c t t t a a g a a g g a g a t a t a c a t a t g c a c c a t c a t c a t c a t c a t t c t t c t g g a a a a c a a a t t g a a a t t c t t c c t c t a t a t g t a t a c g t g g t a g t a g t a g t a g t a a g a a g a c c

BstBI 5160 1 5 LVPRGS GM 1 5 10 KETAAAKFERQH thrombin site S-Tag t c t g g t g c c a c g c g g t t c t g g t a t g a a a g a a a c c g c t g c t g c t a a a t t c g a a c g c c a g c a a g a c c a c g g t g c g c c a a g a c c a t a c t t t c t t t g g c g a c g a c g a t t t a a g c t t g c g g t c g t

PmlIBglII 5220 15 MDS (in frame with S-Tag) PDLKDKQKRKKERTWAE S-Tag c a t g g a c a g c c c a g a t c t g a a g g a c a a a c a g a a g a g g a a g a a g g a g c g c a c g t g g g c c g a g t a c c t g t c g g g t c t a g a c t t c c t g t t t g t c t t c t c c t t c t t c c t c g c g t g c a c c c g g c t

BspMIBfuAI 5280 (in frame with S-Tag) AARLVLENYSDAPMTPKQIL g g c c g c g c g c c t g g t g t t a g a a a a c t a c t c a g a t g c t c c a a t g a c a c c a a a a c a g a t t c t c c g g c g c g c g g a c c a c a a t c t t t t g a t g a g t c t a c g a g g t t a c t g t g g t t t t g t c t a a g a

PstI 5340 (in frame with S-Tag) QVIEAEGLKEMRSGTSPLAC g c a g g t c a t a g a g g c a g a a g g a c t g a a g g a a a t g a g a a g t g g g a c a t c c c c t c t t g c g t g c g t c c a g t a t c t c c g t c t t c c t g a c t t c c t t t a c t c t t c a c c c t g t a g g g g a g a a c g c a c

5400 (in frame with S-Tag) LNAMLHSNSRGGEGLFYKLP c c t c a a t g c c a t g c t a c a t t c c a a c t c a a g a g g a g g a g a a g g g c t g t t t t a t a a a t t a c c g g a g t t a c g g t a c g a t g t a a g g t t g a g t t c t c c t c c t c t t c c c g a c a a a a t a t t t a a t g g

PaeR7IPspXIXhoI 5460 (in frame with S-Tag) GRISLFTL 1 5 DYKDDDDK * 1 H FLAG 6xHis t g g c c g c a t t a g t c t t t t c a c a c t c g a t t a c a a g g a t g a c g a t g a c a a g t g a c t c g a g c a a c c g g c g t a a t c a g a a a a g t g t g a g c t a a t g t t c c t a c t g c t a c t g t t c a c t g a g c t c g t

5520 5 HHHHH * 6xHis c c a c c a c c a c c a c c a c t g a g a t c c g g c t g c t a a c a a a g c c c g a a a g g a a g c t g a g t t g g c g g t g g t g g t g g t g g t g a c t c t a g g c c g a c g a t t g t t t c g g g c t t t c c t t c g a c t c a a c c g

StyIBlpI 5580 T7 terminator G G C G A C T C G T T A T T G A T C G T7 Term t g c t g c c a c c g c t g a g c a a t a a c t a g c a t a a c c c c t t g g g g c c t c t a a a c g g g t c t t g a g a c g a c g g t g g c g a c t c g t t a t t g a t c g t a t t g g g g a a c c c c g g a g a t t t g c c c a g a a c t c

3ʹ 5ʹ 5615 T7 terminator g g g t t t t t t g c t g a a a g g a g g a a c t a t a t c c g g a t c c c a a a a a a c g a c t t t c c t c c t t g a t a t a g g c c t a

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.