Addgene Human USP30 (64-502, construct 2, MG-26-18) - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: Human USP30 (64-502, construct 2, MG-26-18)


Map View

2000 1500 1000 500 End (2104) XbaI (2067) HindIII (2061) BlpI (1951) BsaAI (1942) NsiI (1898) BspEI (1830) AvaI - BsoBI (1776) AgeI (1716) PspFI (1630) PflMI - BseYI (1626) BsaBI (1606) BspHI (1569) StyI - NcoI (1548) BtsI - BtsαI (1513) BmrI (1501) PvuII (1485) AleI (1474) BanI (1432) Bpu10I (1303) BsrDI (1257) BssSI - BssSαI (1094) BstEII (945) ApoI - EcoRI (844) MflI * - BstYI (702) pGEX 5' (657 .. 679) ScaI (619) TatI (617) SwaI (473) BstBI (443) BsrBI (337) MscI (253) EaeI (251) BtgZI (164) EarI - SapI - BspQI (125) Start (0) HRV 3C site GST HRV 3C site 6xHis Human USP30 (64-502, construct 2, MG-26-18) 2104 bp

Sequence View

Start (0) 60 1 5 LEVLFQGP 1 5 MSPIL HRV 3C site GST HHHHSSGLEVLFQGPMSPIL c a t c a t c a t c a c a g c a g c g g t c t g g a a g t t c t g t t t c a g g g c c c g a t g t c c c c t a t a c t a g t a g t a g t a g t g t c g t c g c c a g a c c t t c a a g a c a a a g t c c c g g g c t a c a g g g g a t a t g a t

120 10 15 20 25 GYWKIKGLVQPTRLLLEYLE GST GYWKIKGLVQPTRLLLEYLE g g t t a t t g g a a a a t t a a g g g c c t t g t g c a a c c c a c t c g a c t t c t t t t g g a a t a t c t t g a a c c a a t a a c c t t t t a a t t c c c g g a a c a c g t t g g g t g a g c t g a a g a a a a c c t t a t a g a a c t t

BtgZIBspQISapIEarI 180 30 35 40 45 EKYEEHLYERDEGDKWRNKK GST EKYEEHLYERDEGDKWRNKK g a a a a a t a t g a a g a g c a t t t g t a t g a g c g c g a t g a a g g t g a t a a a t g g c g a a a c a a a a a g c t t t t t a t a c t t c t c g t a a a c a t a c t c g c g c t a c t t c c a c t a t t t a c c g c t t t g t t t t t c

240 50 55 60 65 FELGLEFPNLPYYIDGDVKL GST FELGLEFPNLPYYIDGDVKL t t t g a a t t g g g t t t g g a g t t t c c c a a t c t t c c t t a t t a t a t t g a t g g t g a t g t t a a a t t a a a a c t t a a c c c a a a c c t c a a a g g g t t a g a a g g a a t a a t a t a a c t a c c a c t a c a a t t t a a t

EaeIMscI 300 70 75 80 85 TQSMAIIRYIADKHNMLGGC GST TQSMAIIRYIADKHNMLGGC a c a c a g t c t a t g g c c a t c a t a c g t t a t a t a g c t g a c a a g c a c a a c a t g t t g g g t g g t t g t t g t g t c a g a t a c c g g t a g t a t g c a a t a t a t c g a c t g t t c g t g t t g t a c a a c c c a c c a a c a

BsrBI 360 90 95 100 105 PKERAEISMLEGAVLDIRYG GST PKERAEISMLEGAVLDIRYG c c a a a a g a g c g t g c a g a g a t t t c a a t g c t t g a a g g a g c g g t t t t g g a t a t t a g a t a c g g t g g t t t t c t c g c a c g t c t c t a a a g t t a c g a a c t t c c t c g c c a a a a c c t a t a a t c t a t g c c a

420 110 115 120 125 VSRIAYSKDFETLKVDFLSK GST VSRIAYSKDFETLKVDFLSK g t t t c g a g a a t t g c a t a t a g t a a a g a c t t t g a a a c t c t c a a a g t t g a t t t t c t t a g c a a g c a a a g c t c t t a a c g t a t a t c a t t t c t g a a a c t t t g a g a g t t t c a a c t a a a a g a a t c g t t c

SwaIBstBI 480 130 135 140 145 LPEMLKMFEDRLCHKTYLNG GST LPEMLKMFEDRLCHKTYLNG c t a c c t g a a a t g c t g a a a a t g t t c g a a g a t c g t t t a t g t c a t a a a a c a t a t t t a a a t g g t g a t g g a c t t t a c g a c t t t t a c a a g c t t c t a g c a a a t a c a g t a t t t t g t a t a a a t t t a c c a

540 150 155 160 165 DHVTHPDFMLYDALDVVLYM GST DHVTHPDFMLYDALDVVLYM g a t c a t g t a a c c c a t c c t g a c t t c a t g t t g t a t g a c g c t c t t g a t g t t g t t t t a t a c a t g c t a g t a c a t t g g g t a g g a c t g a a g t a c a a c a t a c t g c g a g a a c t a c a a c a a a a t a t g t a c

600 170 175 180 185 DPMCLDAFPKLVCFKKRIEA GST DPMCLDAFPKLVCFKKRIEA *NTKFFTNFS g a c c c a a t g t g c c t g g a t g c g t t c c c a a a a t t a g t t t g t t t t a a a a a a c g t a t t g a a g c t c t g g g t t a c a c g g a c c t a c g c a a g g g t t t t a a t c a a a c a a a a t t t t t t g c a t a a c t t c g a

TatIScaI 660 190 195 200 205 IPQIDKYLKSSKYIAWPLQG GST G G G C pGEX 5' IPQIDKYLKSSKYIAWPLQG DWLNILVQFGALIYCPRQLA a t c c c a c a a a t t g a t a a g t a c t t g a a a t c c a g c a a g t a t a t a g c a t g g c c t t t g c a g g g c t a g g g t g t t t a a c t a t t c a t g a a c t t t a g g t c g t t c a t a t a t c g t a c c g g a a a c g t c c c g

BstYIMflI* 720 210 215 WQATFGGGDHPPK (in frame with GST) SDLSSG 1 L GST HRV 3C site T G G C A A G C C A C G T T T G G T G pGEX 5' WQATFGGGDHPPKSDLSSGL PLGRKTTAVMRWFRIQAATQ t g g c a a g c c a c g t t t g g t g g t g g c g a c c a t c c t c c a a a a t c g g a t c t g a g c a g c g g t c t g a c c g t t c g g t g c a a a c c a c c a c c g c t g g t a g g a g g t t t t a g c c t a g a c t c g t c g c c a g a c

780 5 EVLFQGP (in frame with HRV 3C site) KGLVPGLVNLGNT HRV 3C site EVLFQGPKGLVPGLVNLGNT FNQKLARFTQNRTQNIQTIG g a a g t t c t g t t t c a g g g c c c g a a a g g t c t g g t t c c g g g t c t g g t t a a t c t g g g t a a t a c c c t t c a a g a c a a a g t c c c g g g c t t t c c a g a c c a a g g c c c a g a c c a a t t a g a c c c a t t a t g g

840 (in frame with HRV 3C site) CFMNSLLQGLSACPAFIRWL CFMNSLLQGLSACPAFIRWL TKHIAQQLTQACTRCKNTPQ t g t t t t a t g a a t a g c c t g c t g c a g g g t c t g a g c g c a t g t c c g g c a t t t a t t c g t t g g c t g a c a a a a t a c t t a t c g g a c g a c g t c c c a g a c t c g c g t a c a g g c c g t a a a t a a g c a a c c g a c

EcoRIApoI 900 (in frame with HRV 3C site) EEFTSQYSRDQKEPPSHQYL EEFTSQYSRDQKEPPSHQYL FFEGALIATILFFRRAMLIQ g a a g a a t t c a c c a g c c a g t a t a g c c g t g a t c a g a a a g a a c c g c c t a g c c a t c a g t a t c t g c t t c t t a a g t g g t c g g t c a t a t c g g c a c t a g t c t t t c t t g g c g g a t c g g t a g t c a t a g a c

BstEII 960 (in frame with HRV 3C site) SLTLLHLLKALSCQEVTDDE SLTLLHLLKALSCQEVTDDE AQGQQMQQFCQATLLNGIIF a g c c t g a c c c t g c t g c a t c t g c t g a a a g c a c t g a g c t g t c a a g a g g t t a c c g a t g a t g a a t c g g a c t g g g a c g a c g t a g a c g a c t t t c g t g a c t c g a c a g t t c t c c a a t g g c t a c t a c t t

1020 (in frame with HRV 3C site) VLDASCLLDVLRMYRWQISS VLDASCLLDVLRMYRWQISS NQICATQQINQTHITPLNAA g t t c t g g a t g c a a g c t g t c t g c t g g a t g t t c t g c g t a t g t a t c g t t g g c a g a t t a g c a g c c a a g a c c t a c g t t c g a c a g a c g a c c t a c a a g a c g c a t a c a t a g c a a c c g t c t a a t c g t c g

1080 (in frame with HRV 3C site) FEEQDAHELFHVITSSLEDE FEEQDAHELFHVITSSLEDE KFFLICMFQKMNNGAAQFIF t t t g a a g a a c a g g a t g c a c a t g a a c t g t t t c a t g t t a t t a c c a g c a g c c t g g a a g a t g a a a a a c t t c t t g t c c t a c g t g t a c t t g a c a a a g t a c a a t a a t g g t c g t c g g a c c t t c t a c t t

BssSαIBssSI 1140 (in frame with HRV 3C site) RDRQPRVTHLFDVHSLEQQS RDRQPRVTHLFDVHSLEQQS TITLRTNGVQKINMAQFLLA c g t g a t c g t c a g c c t c g t g t t a c c c a c c t g t t t g a t g t t c a t a g c c t g g a a c a g c a g a g c g c a c t a g c a g t c g g a g c a c a a t g g g t g g a c a a a c t a c a a g t a t c g g a c c t t g t c g t c t c g

1200 (in frame with HRV 3C site) EITPKQITCRTRGSPHPTSN EITPKQITCRTRGSPHPTSN FNGRFLNGTTGTTTRMRGAI g a a a t t a c c c c g a a a c a a a t t a c c t g t c g t a c c c g t g g t a g t c c g c a t c c g a c c a g c a a t c t t t a a t g g g g c t t t g t t t a a t g g a c a g c a t g g g c a c c a t c a g g c g t a g g c t g g t c g t t a

BsrDI 1260 (in frame with HRV 3C site) HWKSQHPFHGRLTSNMVCKH HWKSQHPFHGRLTSNMVCKH MPFALMRKMTTQGTIHNTFM c a t t g g a a a a g c c a g c a t c c g t t t c a t g g t c g t c t g a c c a g t a a t a t g g t t t g t a a a c a t g t a a c c t t t t c g g t c g t a g g c a a a g t a c c a g c a g a c t g g t c a t t a t a c c a a a c a t t t g t a

Bpu10I 1320 (in frame with HRV 3C site) CEHQSPVRFDTFDSLSLSIP CEHQSPVRFDTFDSLSLSIP AFM*NR t g c g a a c a t c a g a g t c c g g t t c g t t t t g a t a c c t t t g a t a g c c t g a g c c t g t c a a t t c c g a c g c t t g t a g t c t c a g g c c a a g c a a a a c t a t g g a a a c t a t c g g a c t c g g a c a g t t a a g g c

1380 (in frame with HRV 3C site) AATWGHPLTLDHCLHHFISS AATWGHPLTLDHCLHHFISS CCGPTMRQGQIMTQMMENAA g c a g c a a c c t g g g g t c a t c c g c t g a c c c t g g a t c a t t g t c t g c a t c a t t t c a t t a g c a g c c g t c g t t g g a c c c c a g t a g g c g a c t g g g a c c t a g t a a c a g a c g t a g t a a a g t a a t c g t c g

BanI 1440 (in frame with HRV 3C site) ESVRDVVCDNCTKIEAKGTL ESVRDVVCDNCTKIEAKGTL FANTINNTIVAGFNFCFAGQ g a a a g c g t t c g t g a t g t t g t t t g t g a t a a c t g c a c c a a a a t t g a a g c a a a a g g c a c c c t g c t t t c g c a a g c a c t a c a a c a a a c a c t a t t g a c g t g g t t t t a a c t t c g t t t t c c g t g g g a c

PvuIIAleI 1500 (in frame with HRV 3C site) NGEKVEHQRTTFVKQLKLGK NGEKVEHQRTTFVKQLKLGK ITFFNFMLTGGKHFLQFQTF a a t g g t g a a a a a g t t g a a c a t c a g c g t a c c a c c t t t g t g a a a c a g c t g a a a c t g g g t a a a t t a c c a c t t t t t c a a c t t g t a g t c g c a t g g t g g a a a c a c t t t g t c g a c t t t g a c c c a t t t

NcoIStyIBtsαIBtsIBmrI 1560 (in frame with HRV 3C site) LPQCLCIHLQRLSWSSHGTP LPQCLCIHLQRLSWSSHGTP QRLTQTNMQLTQRPAAMTCR c t g c c g c a g t g t c t g t g t a t t c a t c t g c a g c g t c t g t c t t g g a g c a g c c a t g g t a c a c c g g a c g g c g t c a c a g a c a c a t a a g t a g a c g t c g c a g a c a g a a c c t c g t c g g t a c c a t g t g g c

BsaBIBspHI 1620 (in frame with HRV 3C site) LKRHEHVQFNEFLMMDIYKY LKRHEHVQFNEFLMMDIYKY QFTMFMHLKILEQHHVDIFV c t g a a a c g t c a t g a a c a t g t g c a g t t t a a t g a g t t c c t g a t g a t g g a c a t c t a t a a a t a c g a c t t t g c a g t a c t t g t a c a c g t c a a a t t a c t c a a g g a c t a c t a c c t g t a g a t a t t t a t g

BseYIPflMIPspFI 1680 (in frame with HRV 3C site) HLLGHKPSQHNPKLNKNPGP HLLGHKPSQHNPKLNKNPGP MQQAMFRALMIRFQVFIRTR c a t c t g c t g g g c c a t a a a c c g a g c c a g c a t a a t c c g a a a c t g a a c a a a a a t c c g g g t c c g g t a g a c g a c c c g g t a t t t g g c t c g g t c g t a t t a g g c t t t g a c t t g t t t t t a g g c c c a g g c

AgeI 1740 (in frame with HRV 3C site) TLELQDGPGAPTPVLNQPGA TLELQDGPGAPTPVLNQPGA GQFQLITRTCRCRNQILRAC a c c c t g g a a c t g c a g g a t g g t c c g g g t g c a c c g a c a c c g g t t c t g a a t c a g c c t g g c g c a t g g g a c c t t g a c g t c c t a c c a g g c c c a c g t g g c t g t g g c c a a g a c t t a g t c g g a c c g c g t

BsoBIAvaI 1800 (in frame with HRV 3C site) PKTQIFMNGACSPSLLPTLS PKTQIFMNGACSPSLLPTLS RFGLNKHVTCTARTQQRGQT c c g a a a a c c c a g a t t t t t a t g a a c g g t g c a t g t a g c c c g a g t c t g c t g c c g a c c c t g a g t g g c t t t t g g g t c t a a a a a t a c t t g c c a c g t a c a t c g g g c t c a g a c g a c g g c t g g g a c t c a

BspEI 1860 (in frame with HRV 3C site) APMPFPLPVVPDYSSSTYLF APMPFPLPVVPDYSSSTYLF CRHRKRQRNNRIIAATGVQK g c a c c g a t g c c g t t t c c g c t g c c g g t t g t t c c g g a t t a t a g c a g c a g t a c c t a c c t g t t t c g t g g c t a c g g c a a a g g c g a c g g c c a a c a a g g c c t a a t a t c g t c g t c a t g g a t g g a c a a a

NsiI 1920 (in frame with HRV 3C site) RLMAVVVHHGDMHSGHFVTY RLMAVVVHHGDMHSGHFVTY TQHCNNNMMTIHMATM c g t c t g a t g g c a g t t g t t g t t c a t c a t g g t g a t a t g c a t a g c g g t c a t t t t g t t a c c t a t g c a g a c t a c c g t c a a c a a c a a g t a g t a c c a c t a t a c g t a t c g c c a g t a a a a c a a t g g a t a

BlpIBsaAI 1980 (in frame with HRV 3C site) RRSPPSARNPLSTSNQWLWV RRSPPSARNPLSTSNQWLWV c g t c g t a g c c c t c c g a g c g c a c g t a a t c c g c t g a g c a c c a g t a a t c a g t g g c t g t g g g t t g c a g c a t c g g g a g g c t c g c g t g c a t t a g g c g a c t c g t g g t c a t t a g t c a c c g a c a c c c a a

2040 (in frame with HRV 3C site) SDDTVRKASLQEVLSSSAYL SDDTVRKASLQEVLSSSAYL a g t g a t g a t a c c g t t c g t a a a g c a a g c c t g c a a g a a g t t c t g a g c a g c a g c g c a t a t c t g t c a c t a c t a t g g c a a g c a t t t c g t t c g g a c g t t c t t c a a g a c t c g t c g t c g c g t a t a g a c

XbaIHindIII 2100 (in frame with HRV 3C site) LFYERV* 1 5 HHHHHH 6xHis LFYERV* c t g t t t t a c g a g c g t g t t t a a a g c t t t c t a g a c c a t t t a a a c a c c a c c a c c a c c a c c a c t g a c a a a a t g c t c g c a c a a a t t t c g a a a g a t c t g g t a a a t t t g t g g t g g t g g t g g t g g t g a

End (2104) 2104 * g a g a c t c t

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

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Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.