Addgene pAAV-TRE-mTurquoise2-WPRE Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pAAV-TRE-mTurquoise2-WPRE Sequencing Result

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2000 1500 1000 500 End (2312) BsaBI * (2120) DraI (2080) MfeI (2028) HpaI (2019) PsiI (1999) SV40pA-R (1963 .. 1982) EBV-rev (1909 .. 1928) BsiEI (1879) NotI - EagI (1876) pBluescriptKS (1853 .. 1869) AccI (1860) SalI (1859) MmeI (1801) EarI (1793) BbsI (1789) NaeI (1775) NgoMIV (1773) SacII (1764) AatII (1714) ZraI (1712) BsrDI (1508) PflMI (1502) ApaLI (1470) WPRE-R (1314 .. 1334) BciVI (1330) HindIII (1249) BsrGI (1237) EGFP-C (1181 .. 1202) BlpI (1149) BmrI (1133) Bpu10I (1131) PflFI - Tth111I (975) EXFP-R (834 .. 853) DrdI (719) BssSI BssSαI (708) EGFP-N (573 .. 594) BanI (563) NcoI (526) EcoRI (516) SacI (487) Eco53kI (485) LNCX (484 .. 508) StuI (470) tet operator tet operator tet operator tet operator tet operator tet operator tet operator BglII (186) Start (0) AAV2 ITR minimal CMV promoter mTurquoise2 WPRE KS primer SV40 poly(A) signal AAV2 ITR Kozak sequence Factor Xa site pAAV-TRE-mTurquoise2-WPRE 2312 bp

Sequence View

Start (0) 5ʹ 3ʹ 60 AAV2 ITR g c t g c g c g c t c g c t c g c t c a c t g a g g c c g c c c g g g c a a a g c c c g g g c g t c g g g c g a c c t t c g a c g c g c g a g c g a g c g a g t g a c t c c g g c g g g c c c g t t t c g g g c c c g c a g c c c g c t g g a a

120 AAV2 ITR t g g t c g c c c g g c c t c a g t g a g c g a g c g a g c g c g c a g a g a g g g a g t g g c c a a c t c c a t c a c a c c a g c g g g c c g g a g t c a c t c g c t c g c t c g c g c g t c t c t c c c t c a c c g g t t g a g g t a g t g

180 AAV2 ITR t a g g g g t t c c t t g t a g t t a a t g a t t a a c c c g c c a t g c t a c t t a t c t a c g t a g c c a t g c t c a t c c c c a a g g a a c a t c a a t t a c t a a t t g g g c g g t a c g a t g a a t a g a t g c a t c g g t a c g a g

BglII 240 tet operator t a g g a a g a t c t c g a a t t g g t t t a c t c c c t a t c a g t g a t a g a g a a c g t a t g t c g a g t t t a c a t c c t t c t a g a g c t t a a c c a a a t g a g g g a t a g t c a c t a t c t c t t g c a t a c a g c t c a a a t g

300 tet operator tet operator MSSLLPISDRERM t c c c t a t c a g t g a t a g a g a a c g a t g t c g a g t t t a c t c c c t a t c a g t g a t a g a g a a c g t a t a g g g a t a g t c a c t a t c t c t t g c t a c a g c t c a a a t g a g g g a t a g t c a c t a t c t c t t g c a t a

360 tet operator tet operator SSLLPISDRERMSSLLPISD g t c g a g t t t a c t c c c t a t c a g t g a t a g a g a a c g t a t g t c g a g t t t a c t c c c t a t c a g t g a c a g c t c a a a t g a g g g a t a g t c a c t a t c t c t t g c a t a c a g c t c a a a t g a g g g a t a g t c a c t

420 tet operator tet operator tet operator RERMSSLSLSVIENVCRVYS t a g a g a a c g t a t g t c g a g t t t a t c c c t a t c a g t g a t a g a g a a c g t a t g t c g a g t t t a c t c a t c t c t t g c a t a c a g c t c a a a t a g g g a t a g t c a c t a t c t c t t g c a t a c a g c t c a a a t g a g

StuI 480 tet operator minimal CMV promoter LSVIENVCRGRRVRWEAYIS c c t a t c a g t g a t a g a g a a c g t a t g t c g a g g t a g g c g t g t a c g g t g g g a g g c c t a t a t a a g g g a t a g t c a c t a t c t c t t g c a t a c a g c t c c a t c c g c a c a t g c c a c c c t c c g g a t a t a t t c

NcoIEcoRIEco53kISacI 540 1 M 1a V SK minimal CMV promoter mTurquoise2 Kozak sequence A G C T C G T T T A G T G A A C C G T C A G A T C LNCX RARLVNRQIAWRIRHHGEQG MVSK c a g a g c t c g t t t a g t g a a c c g t c a g a t c g c c t g g a g a a t t c g c c a c c a t g g t g a g c a a g g g t c t c g a g c a a a t c a c t t g g c a g t c t a g c g g a c c t c t t a a g c g g t g g t a c c a c t c g t t c c

BanI 600 5 10 15 20 GEELFTGVVPILVELDGDVN mTurquoise2 G A C C A G C T C G A C C T G C C G C T G C EGFP-N RGAVHRGGAHPGRAGRRRKR GEELFTGVVPILVELDGDVN g c g a g g a g c t g t t c a c c g g g g t g g t g c c c a t c c t g g t c g a g c t g g a c g g c g a c g t a a a c g c g c t c c t c g a c a a g t g g c c c c a c c a c g g g t a g g a c c a g c t c g a c c t g c c g c t g c a t t t g c

660 25 30 35 40 GHKFSVSGEGEGDATYGKLT mTurquoise2 PQVQRVRRGRGRCHLRQADP GHKFSVSGEGEGDATYGKLT g c c a c a a g t t c a g c g t g t c c g g c g a g g g c g a g g g c g a t g c c a c c t a c g g c a a g c t g a c c c c g g t g t t c a a g t c g c a c a g g c c g c t c c c g c t c c c g c t a c g g t g g a t g c c g t t c g a c t g g g

DrdIBssSαIBssSI 720 45 50 55 60 LKFICTTGKLPVPWPTLVTT mTurquoise2 EVHLHHRQAARALAHPRDHP LKFICTTGKLPVPWPTLVTT t g a a g t t c a t c t g c a c c a c c g g c a a g c t g c c c g t g c c c t g g c c c a c c c t c g t g a c c a c c c a c t t c a a g t a g a c g t g g t g g c c g t t c g a c g g g c a c g g g a c c g g g t g g g a g c a c t g g t g g g

780 65 70 75 80 LSWGVQCFARYPDHMKQHDF mTurquoise2 VLGRAVLRPLPRPHEAARLL LSWGVQCFARYPDHMKQHDF t g t c c t g g g g c g t g c a g t g c t t c g c c c g c t a c c c c g a c c a c a t g a a g c a g c a c g a c t t c t a c a g g a c c c c g c a c g t c a c g a a g c g g g c g a t g g g g c t g g t g t a c t t c g t c g t g c t g a a g a

840 85 90 95 100 FKSAMPEGYVQERTIFFKDD mTurquoise2 C T G C T G C EXFP-R QVRHARRLRPGAHHLLQGRR FKSAMPEGYVQERTIFFKDD t c a a g t c c g c c a t g c c c g a a g g c t a c g t c c a g g a g c g c a c c a t c t t c t t c a a g g a c g a c g a g t t c a g g c g g t a c g g g c t t c c g a t g c a g g t c c t c g c g t g g t a g a a g a a g t t c c t g c t g c

900 105 110 115 120 GNYKTRAEVKFEGDTLVNRI mTurquoise2 C G T T G A T G T T C T G EXFP-R QLQDPRRGEVRGRHPGEPHR GNYKTRAEVKFEGDTLVNRI g c a a c t a c a a g a c c c g c g c c g a g g t g a a g t t c g a g g g c g a c a c c c t g g t g a a c c g c a t c g c g t t g a t g t t c t g g g c g c g g c t c c a c t t c a a g c t c c c g c t g t g g g a c c a c t t g g c g t a g c

960 125 130 135 140 ELKGIDFKEDGNILGHKLEY mTurquoise2 AEGHRLQGGRQHPGAQAGVQ ELKGIDFKEDGNILGHKLEY a g c t g a a g g g c a t c g a c t t c a a g g a g g a c g g c a a c a t c c t g g g g c a c a a g c t g g a g t a c a t c g a c t t c c c g t a g c t g a a g t t c c t c c t g c c g t t g t a g g a c c c c g t g t t c g a c c t c a t g t

Tth111IPflFI 1020 145 150 155 160 NYFSDNVYITADKQKNGIKA mTurquoise2 LL* NYFSDNVYITADKQKNGIKA a c t a c t t t a g c g a c a a c g t c t a t a t c a c c g c c g a c a a g c a g a a g a a c g g c a t c a a g g c c a t g a t g a a a t c g c t g t t g c a g a t a t a g t g g c g g c t g t t c g t c t t c t t g c c g t a g t t c c g g t

1080 165 170 175 180 NFKIRHNIEDGGVQLADHYQ mTurquoise2 NFKIRHNIEDGGVQLADHYQ a c t t c a a g a t c c g c c a c a a c a t c g a g g a c g g c g g c g t g c a g c t c g c c g a c c a c t a c c a g c t g a a g t t c t a g g c g g t g t t g t a g c t c c t g c c g c c g c a c g t c g a g c g g c t g g t g a t g g t c g

Bpu10IBmrI 1140 185 190 195 200 QNTPIGDGPVLLPDNHYLST mTurquoise2 QNTPIGDGPVLLPDNHYLST a g a a c a c c c c c a t c g g c g a c g g c c c c g t g c t g c t g c c c g a c a a c c a c t a c c t g a g c a c c c t c t t g t g g g g g t a g c c g c t g c c g g g g c a c g a c g a c g g g c t g t t g g t g a t g g a c t c g t g g g

BlpI 1200 205 210 215 220 QSKLSKDPNEKRDHMVLLEF mTurquoise2 C A T G G T C C T G C T G G A G T T C G EGFP-C QSKLSKDPNEKRDHMVLLEF a g t c c a a g c t g a g c a a a g a c c c c a a c g a g a a g c g c g a t c a c a t g g t c c t g c t g g a g t t c g t c a g g t t c g a c t c g t t t c t g g g g t t g c t c t t c g c g c t a g t g t a c c a g g a c g a c c t c a a g c

HindIIIBsrGI 1260 225 230 235 VTAAGITLGMDELYK* mTurquoise2 T G EGFP-C VTAAGITLGMDELYK* t g a c c g c c g c c g g g a t c a c t c t c g g c a t g g a c g a g c t g t a c a a g t a a g a a g c t t a t c g a t a c t g g c g g c g g c c c t a g t g a g a g c c g t a c c t g c t c g a c a t g t t c a t t c t t c g a a t a g c t a

1320 WPRE A C A A C G A WPRE-R a a t c a a c c t c t g g a t t a c a a a a t t t g t g a a a g a t t g a c t g g t a t t c t t a a c t a t g t t g c t t t a g t t g g a g a c c t a a t g t t t t a a a c a c t t t c t a a c t g a c c a t a a g a a t t g a t a c a a c g a

BciVI 1380 WPRE G G A A A A T G C G A T A C WPRE-R MPLYHAIASR MLLLPV c c t t t t a c g c t a t g t g g a t a c g c t g c t t t a a t g c c t t t g t a t c a t g c t a t t g c t t c c c g t g g a a a a t g c g a t a c a c c t a t g c g a c g a a a t t a c g g a a a c a t a g t a c g a t a a c g a a g g g c a

1440 WPRE MAFIFSSLYKSWLLSLYEEL WLSFSPPCINPGCCLFMRSC a t g g c t t t c a t t t t c t c c t c c t t g t a t a a a t c c t g g t t g c t g t c t c t t t a t g a g g a g t t g t a c c g a a a g t a a a a g a g g a g g a a c a t a t t t a g g a c c a a c g a c a g a g a a a t a c t c c t c a a c

ApaLI 1500 WPRE WPVVRQRGVVCTVFADATPT GPLSGNVAWCALCLLTQPPL t g g c c c g t t g t c a g g c a a c g t g g c g t g g t g t g c a c t g t g t t t g c t g a c g c a a c c c c c a c t a c c g g g c a a c a g t c c g t t g c a c c g c a c c a c a c g t g a c a c a a a c g a c t g c g t t g g g g g t g a

BsrDIPflMI 1560 WPRE GWGIATTCQLLSGTFAFPLP VGALPPPVSSFPGLSLSPSL g g t t g g g g c a t t g c c a c c a c c t g t c a g c t c c t t t c c g g g a c t t t c g c t t t c c c c c t c c c t c c a a c c c c g t a a c g g t g g t g g a c a g t c g a g g a a a g g c c c t g a a a g c g a a a g g g g g a g g g a

1620 WPRE IATAELIAACLARCWTGARL LPRRNSSPPALPAAGQGLGC a t t g c c a c g g c g g a a c t c a t c g c c g c c t g c c t t g c c c g c t g c t g g a c a g g g g c t c g g c t g t a a c g g t g c c g c c t t g a g t a g c g g c g g a c g g a a c g g g c g a c g a c c t g t c c c c g a g c c g a c

1680 WPRE (in frame with Factor Xa site) *RGKRPQE LGTDNSVVLSGKSSSFPWLL WALTIPWCCRGNHRPFLGCS t t g g g c a c t g a c a a t t c c g t g g t g t t g t c g g g g a a a t c a t c g t c c t t t c c t t g g c t g c t c a a c c c g t g a c t g t t a a g g c a c c a c a a c a g c c c c t t t a g t a g c a g g a a a g g a a c c g a c g a g

ZraIAatII 1740 WPRE (in frame with Factor Xa site) GTNGGPNQAPRGEAVDR RGE Factor Xa site ACVATWILRGTSFCYVPSAL PVLPPGFCAGRPSATSLRPS g c c t g t g t t g c c a c c t g g a t t c t g c g c g g g a c g t c c t t c t g c t a c g t c c c t t c g g c c c t c c g g a c a c a a c g g t g g a c c t a a g a c g c g c c c t g c a g g a a g a c g a t g c a g g g a a g c c g g g a g

EarIBbsISacIINgoMIVNaeI 1800 WPRE 1 I Factor Xa site NPADLPSRGLLPALRPLPRL IQRTFLPAACCRLCGLFRVF a a t c c a g c g g a c c t t c c t t c c c g c g g c c t g c t g c c g g c t c t g c g g c c t c t t c c g c g t c t t t t a g g t c g c c t g g a a g g a a g g g c g c c g g a c g a c g g c c g a g a c g c c g g a g a a g g c g c a g a a

SalIMmeI 1860 WPRE KS primer C T A T G G C A pBluescriptKS RLRPQTSRISLWAASPHRYR AFALRRVGSPFGPPPRIDTV c g c c t t c g c c c t c a g a c g a g t c g g a t c t c c c t t t g g g c c g c c t c c c c g c a t c g a t a c c g t g c g g a a g c g g g a g t c t g c t c a g c c t a g a g g g a a a c c c g g c g g a g g g g c g t a g c t a t g g c a

EagINotIBsiEIAccI 1920 KS primer SV40 poly(A) signal G C T G G A G C T pBluescriptKS C T A C T C A A A C C T EBV-rev RPRPGRPLRADMIRYIDEFG DLDPGGRFEQT* c g a c c t c g a c c c g g g c g g c c g c t t c g a g c a g a c a t g a t a a g a t a c a t t g a t g a g t t t g g a g c t g g a g c t g g g c c c g c c g g c g a a g c t c g t c t g t a c t a t t c t a t g t a a c t a c t c a a a c c t

1980 SV40 poly(A) signal G A A A T T T G T G A T G C T A T T SV40pA-R G T T T G G T G EBV-rev QTTTRMQ* c a a a c c a c a a c t a g a a t g c a g t g a a a a a a a t g c t t t a t t t g t g a a a t t t g t g a t g c t a t t g t t t g g t g t t g a t c t t a c g t c a c t t t t t t t a c g a a a t a a a c a c t t t a a a c a c t a c g a t a a

MfeIHpaIPsiI 2040 SV40 poly(A) signal G C SV40pA-R g c t t t a t t t g t a a c c a t t a t a a g c t g c a a t a a a c a a g t t a a c a a c a a c a a t t g c a t t c a t c g a a a t a a a c a t t g g t a a t a t t c g a c g t t a t t t g t t c a a t t g t t g t t g t t a a c g t a a g t a

DraI 2100 t t t a t g t t t c a g g t t c a g g g g g a g a t g t g g g a g g t t t t t t a a a g c a a g t a a a a c c t c t a c a a a t a c a a a g t c c a a g t c c c c c t c t a c a c c c t c c a a a a a a t t t c g t t c a t t t t g g a g a t g

BsaBI* 2160 a a a t g t g g t a a a a t c g a t a a g g a t c t t c c t a g a g c a t g g c t a c g t a g a t a a g t a g c a t g g t t t a c a c c a t t t t a g c t a t t c c t a g a a g g a t c t c g t a c c g a t g c a t c t a t t c a t c g t a c c

2220 AAV2 ITR c g g g t t a a t c a t t a a c t a c a a g g a a c c c c t a g t g a t g g a g t t g g c c a c t c c c t c t c t g c g g c c c a a t t a g t a a t t g a t g t t c c t t g g g g a t c a c t a c c t c a a c c g g t g a g g g a g a g a c g c

2280 AAV2 ITR c g c t c g c t c g c t c a c t g a g g c c g g g c g a c c a a a g g t c g c c c g a c g c c c g g g c t t t g c c c g g c g a g c g a g c g a g t g a c t c c g g c c c g c t g g t t t c c a g c g g g c t g c g g g c c c g a a a c g g g c

End (2312) 3ʹ 5ʹ 2312 AAV2 ITR g g c g g c c t c a g t g a g c g a g c g a g c g c g c a g c t c c g c c g g a g t c a c t c g c t c g c t c g c g c g t c g a

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

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Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.