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Sequence Analyzer: actc1b-mKate2-rab42a


Map View

Amp-R (9227 .. 9246) FspI (8899) pBR322ori-F (7925 .. 7944) L4440 (7674 .. 7691) BspQI - SapI (7668) CAP binding site M13/pUC Reverse (7456 .. 7478) lac operator M13 rev T3 (7404 .. 7424) BssHII (7423) SacI (7390) Eco53kI (7388) SacII (7381) NotI (7369) pBluescriptSK (7351 .. 7367) SK primer AatII (6974) ZraI (6972) SV40pA-R (6623 .. 6642) EBV-rev (6569 .. 6588) KpnI (6534) Acc65I (6530) M13 fwd BmtI (6266) NheI (6262) AsiSI (6232) DraIII (5770) PasI (5344) NcoI (5121) F1ori-F (144 .. 165) PstI (928) SfiI (1049) PspOMI (1050) ApaI (1054) PaeR7I - PspXI - XhoI (1055) M13 rev BstZ17I (1549) PpuMI (2838) AfeI (3120) NgoMIV (3335) NaeI (3337) PacI (4304) Bsu36I (4961) actc1b-mKate2-rab42a 9588 bp

Sequence View

5ʹ 3ʹ 60 c c a c c t a a a t t g t a a g c g t t a a t a t t t t g t t a a a a t t c g c g t t a a a t t t t t g t t a a a t c a g g t g g a t t t a a c a t t c g c a a t t a t a a a a c a a t t t t a a g c g c a a t t t a a a a a c a a t t t a g t

120 g c t c a t t t t t t a a c c a a t a g g c c g a a a t c g g c a a a a t c c c t t a t a a a t c a a a a g a a t a g a c g a g t a a a a a a t t g g t t a t c c g g c t t t a g c c g t t t t a g g g a a t a t t t a g t t t t c t t a t c t

180 G G T C A A A C C T T G T T C T C A G G T G F1ori-F c c g a g a t a g g g t t g a g t g t t g t t c c a g t t t g g a a c a a g a g t c c a c t a t t a a a g a a c g t g g g g c t c t a t c c c a a c t c a c a a c a a g g t c a a a c c t t g t t c t c a g g t g a t a a t t t c t t g c a c c

240 a c t c c a a c g t c a a a g g g c g a a a a a c c g t c t a t c a g g g c g a t g g c c c a c g c t g g c t a a g a a t g a g g t t g c a g t t t c c c g c t t t t t g g c a g a t a g t c c c g c t a c c g g g t g c g a c c g a t t c t t

300 c t c a t c a g c c t c c c c g g t c c a t c t a c c c a c g t a c c a a t g c a c c a a t t g g c c a c a a t g a c g g a g t a g t c g g a g g g g c c a g g t a g a t g g g t g c a t g g t t a c g t g g t t a a c c g g t g t t a c t g c

360 g c t a c t a c a t g g t g c c a t t c c t t c c t c t t t a t a g g a a t g g a g a c t a c c t c c t g t c c a a c a c g a t g a t g t a c c a c g g t a a g g a a g g a g a a a t a t c c t t a c c t c t g a t g g a g g a c a g g t t g t

420 a g g c t c t t g g a t a c g a g t a c g c c t a c c t g t t g g a c c c a g g t c a t t g c a c a a c a c c a g a a a t c c g a g a a c c t a t g c t c a t g c g g a t g g a c a a c c t g g g t c c a g t a a c g t g t t g t g g t c t t t

480 t g c c c t c t g a t c t g c a a a a g a c g t g a a t a t c t g t t c a g a c a c c c a t a t c c a c t c t g t t c c a c g g g a g a c t a g a c g t t t t c t g c a c t t a t a g a c a a g t c t g t g g g t a t a g g t g a g a c a a g g

540 a c a c a g g t c a g a g g t t t g t c c a g g a g t t c t t g a c a g a g g t g t a a a a a g t a c t c a a a a a t t t g t g t c c a g t c t c c a a a c a g g t c c t c a a g a a c t g t c t c c a c a t t t t t c a t g a g t t t t t a a

600 t t a c t c a a g t g a a a g t a c a a g t a c t t a g g g a a a a t t t t a c t c a a t t a a a a g t a a a a g t a t a a t g a g t t c a c t t t c a t g t t c a t g a a t c c c t t t t a a a a t g a g t t a a t t t t c a t t t t c a t a

660 c t g g c t a g a a t c t t a c t t g a g t a a a a g t a a a a a a g t a c t c c a t t a a a a t t g t a c t t g a g t g a c c g a t c t t a g a a t g a a c t c a t t t t c a t t t t t t c a t g a g g t a a t t t t a a c a t g a a c t c a

720 a t t a a g g a a g t a a a a g t a a a a g c a a g a a a g a a a a c t a g a g a t t c t t g t t t a a g c t t t t a a t a a t t c c t t c a t t t t c a t t t t c g t t c t t t c t t t t g a t c t c t a a g a a c a a a t t c g a a a a t t

780 t c t c a a a a a a c a t t a a a t g a a a t g c a t a c a a g g t t t t a t c c t g c t t t a g a a c t g t t t g t a a g a g t t t t t t g t a a t t t a c t t t a c g t a t g t t c c a a a a t a g g a c g a a a t c t t g a c a a a c a t

840 t t t a a t t a t c a a a c t a t a a g a c a g a c a a t c t a a t g c c a g t a c a c g c t a c t c a a a g t t g t a a a a t t a a t a g t t t g a t a t t c t g t c t g t t a g a t t a c g g t c a t g t g c g a t g a g t t t c a a c a t

900 a a a c c t c a g a t t t a a c t t c a g t a g a a g c t g a t t c t c a a a a t t g t t a g t g t c a a g c c t a g c t t t g g a g t c t a a a t t g a a g t c a t c t t c g a c t a a g a g t t t t a a c a a t c a c a g t t c g g a t c g

PstI 960 t c t t t t g g g g c t g a a a a g c a a t c c t g c a g t g c t g a a a a g c c t c t c a c a g g c a g c c g a t g c a g a a a a c c c c g a c t t t t c g t t a g g a c g t c a c g a c t t t t c g g a g a g t g t c c g t c g g c t a c g

1020 g g g a a g a g g t g t a t t a g t c t t g a t a g a g a g g c t g c a a a t a g c a g g a a a c g t g a g c a g a g a c c c t t c t c c a c a t a a t c a g a a c t a t c t c t c c g a c g t t t a t c g t c c t t t g c a c t c g t c t c t

SfiIPspOMIApaIPaeR7IPspXIXhoI 1080 M13 rev c t c c c t g g t g t c t g a a a c a c a g g c c a g a t g g g c c c t c g a g c a g g a a a c a g c t a t g a c c a t g a g g g a c c a c a g a c t t t g t g t c c g g t c t a c c c g g g a g c t c g t c c t t t g t c g a t a c t g g t a

1140 attB4 g a t t a c g c c a a g c t a t c a a c t t t g t a t a g a a a a g t t g g c a c g c g c a g a t t c c g t g t g g g c c t a a t g c g g t t c g a t a g t t g a a a c a t a t c t t t t c a a c c g t g c g c g t c t a a g g c a c a c c c g

1200 c t g g t a a t a t c t t a c c a t t t t a a g c t a t t t t a a g a t t a g g g a a a a a a a t t a a a t a a a a c t g a c c a t t a t a g a a t g g t a a a a t t c g a t a a a a t t c t a a t c c c t t t t t t t a a t t t a t t t t g a

1260 a c t t t a a a a t t a c t t t c c t t a a a a a g c a a c a t a t t t a t t t a g t t t t t g t t t a a c t g g c t c t g a a a t t t t a a t g a a a g g a a t t t t t c g t t g t a t a a a t a a a t c a a a a a c a a a t t g a c c g a g

1320 a a g a t t g t a a t a t t t t a c t t t t a c a t g t t t t t t t t a a g t a c c t a a a t g t g t t c a a a a g t a t t c t a a c a t t a t a a a a t g a a a a t g t a c a a a a a a a a t t c a t g g a t t t a c a c a a g t t t t c a t

1380 a a g t a t g t t a t t g t t t t t t t t t t t t t a a t g t t a g c t a a g c t t t a a a t t g t a a t a t a t t a c t t c a t a c a a t a a c a a a a a a a a a a a a a t t a c a a t c g a t t c g a a a t t t a a c a t t a t a t a a t g

1440 t t t t a a a a g t g a c t t t t t a a a a a t g t a a a t a a a a a a g t a a c t a t a a a t g t g t a a t g c a t g a a a a t t t t c a c t g a a a a a t t t t t a c a t t t a t t t t t t c a t t g a t a t t t a c a c a t t a c g t a c

1500 t t a t t t c t g t g g g t t t t t t a a t t t t c a t c t c a a c t g g g c t t a c a a t t t t a a c a t a a t a a a a a t a a a g a c a c c c a a a a a a t t a a a a g t a g a g t t g a c c c g a a t g t t a a a a t t g t a t t a t t t

BstZ17I 1560 a a t c a a a t c a a a t c a a a t c a g g c c c t t a t t t g t c a c a t a c a c t c t t g t a t a c t g a a a t a a t t a g t t t a g t t t a g t t t a g t c c g g g a a t a a a c a g t g t a t g t g a g a a c a t a t g a c t t t a t t

1620 a c t a a a t g t t g a g a a a a a t t t a a a t a c a c c a a a a t a a c g c a t a c c t t t t t t g t a a t t a c c t g a t t t a c a a c t c t t t t t a a a t t t a t g t g g t t t t a t t g c g t a t g g a a a a a a c a t t a a t g g

1680 t a a g t a a a a c a t t t a g t t c c t t t t t t a a g t g a c t c a a g a t t g t a a t c t a t t a a t t g t a a a a t t c a t t t t g t a a a t c a a g g a a a a a a t t c a c t g a g t t c t a a c a t t a g a t a a t t a a c a t t t

1740 a g a t a a a a c t a g t g a t c a a t c t g a c g t g c t t c t a t g c a g c c t g t g a g a t t a a t t g t g a t g t c t a t t t t g a t c a c t a g t t a g a c t g c a c g a a g a t a c g t c g g a c a c t c t a a t t a a c a c t a c

1800 t t t a a t t a t t a a a c a a t t t a a a t g g a t c a a a c a t t t c a c a t g t g c t t a t t g t g t c c a a c a a a a t t a a t a a t t t g t t a a a t t t a c c t a g t t t g t a a a g t g t a c a c g a a t a a c a c a g g t t g t

1860 a a a c c a g c a g a a g c a c t t a a a a a t a t t g c a t g a c a a c a t g a g t t t t t a a t g t a a a t t a t a t t t g g t c g t c t t c g t g a a t t t t t a t a a c g t a c t g t t g t a c t c a a a a a t t a c a t t t a a t a t

1920 t a t a c t t c c t a a a c g a a g t t a a t c a t a t t t t c t a a a a t a a t a a t g t c a t t g a a a t a t t t a a t a t g a a g g a t t t g c t t c a a t t a g t a t a a a a g a t t t t a t t a t t a c a g t a a c t t t a t a a a t

1980 a a t a a a t g a t a a n c t t t a g t t g a t t c a a c a t a a a a t t g a a a g c a a t a a g g a c t t t t t t t t t t a t t t a c t a t t n g a a a t c a a c t a a g t t g t a t t t t a a c t t t c g t t a t t c c t g a a a a a a a a

2040 t t t t a c a g c g g t g a a g c a a a a g c n t g n a a a t a a t c a t a t a a a t a t a a t a a a t a a a t a a a t a a a a t g t c g c c a c t t c g t t t t c g n a c n t t t a t t a g t a t a t t t a t a t t a t t t a t t t a t t t a

2100 a a a a t a a t g g t t c t t g g t g c a a a t a t g a a c a c t t t a a a t a a a t a t a g a a t a a a t a t g a t c t t t t a t t a c c a a g a a c c a c g t t t a t a c t t g t g a a a t t t a t t t a t a t c t t a t t t a t a c t a g

2160 a a t a a g t c a t a a c g a c g t g t t t t a t g t t g a t t a t a a t t t a a a c a t g t t t c t a a c g c a a g t t t a t t c a g t a t t g c t g c a c a a a a t a c a a c t a a t a t t a a a t t t g t a c a a a g a t t g c g t t c a

2220 t t t a a a a a c t g t t t t a a a g t a t t a t t t t a a g t t a t a a g a t c a a t t t g a c t c a a c a t t t t g a a a t t t t t g a c a a a a t t t c a t a a t a a a a t t c a a t a t t c t a g t t a a a c t g a g t t g t a a a a c

2280 t a g t g a a a a t a t g t t g t g t a a a a t t a a t a t a a t a t a c a c t a t a a a a a t g c a a g g t t c c a c a t c a c t t t t a t a c a a c a c a t t t t a a t t a t a t t a t a t g t g a t a t t t t t a c g t t c c a a g g t g

2340 a c a a t t c a t t c a t g t t g t c c c a a c a c a a a t c g a t t a a g t t a a c a a a t a t a t g t g g a t t g a t g t t a a g t a a g t a c a a c a g g g t t g t g t t t a g c t a a t t c a a t t g t t t a t a t a c a c c t a a c t

2400 a c a t a a a a a a t t a a g t t g t c c c a a t g a a a t c t c a a g a a t t g t g t t g t t t c a g c t c a t t t t t g t a t t t t t t a a t t c a a c a g g g t t a c t t t a g a g t t c t t a a c a c a a c a a a g t c g a g t a a a a

2460 a a a t a a g t a g t t t t a a c a a g t a a a a a a t a a c a t a t t t t t g a g t g t g t a t a t a t a t a t a t t t t t a t t c a t c a a a a t t g t t c a t t t t t t a t t g t a t a a a a a c t c a c a c a t a t a t a t a t a t a a

2520 t a t a t a t t a c a a a a t a t a a t a a t a t a a a a t g t a a a a g a t a t a a a c c a t g c t a c a t a t a t t a t a t a t a a t g t t t t a t a t t a t t a t a t t t t a c a t t t t c t a t a t t t g g t a c g a t g t a t a t a a

2580 t a a g a g c t a t t t t g c a c a t t a c t t t t t t g c a a g c c a t t t g a g t g t t t t t t t a c a a a t a t a a t t c t c g a t a a a a c g t g t a a t g a a a a a a c g t t c g g t a a a c t c a c a a a a a a a t g t t t a t a t

2640 t a t t a g c t a a a t a t a c a a t a t c t t a a a t a t a c a a t t a a t c a g a c a t g c a a a a a a t g c a t t a t a a t c g a t t t a t a t g t t a t a g a a t t t a t a t g t t a a t t a g t c t g t a c g t t t t t t a c g t a a

2700 t a a c c t a c a t a a a a a t a c a t g a t c a a c a t a t g t t t t a t g c t g a t t a c a a c t a a a t c a a a c a t t g g a t g t a t t t t t a t g t a c t a g t t g t a t a c a a a a t a c g a c t a a t g t t g a t t t a g t t t g

2760 g t a a t g a a a t t a a t t t a t a a a a g c g a t t t a a a t a t g t c a a t a t t t t a a g t t a a a a t c a c t c a t t a c t t t a a t t a a a t a t t t t c g c t a a a t t t a t a c a g t t a t a a a a t t c a a t t t t a g t g a

2820 t g a a a c c t a c t a t a a a t g a a c t t a a a t t t a a a a a a t c t c c t t t a a t t c c a a t g c a g c a c t a c t t t g g a t g a t a t t t a c t t g a a t t t a a a t t t t t t a g a g g a a a t t a a g g t t a c g t c g t g a

PpuMI 2880 MVGPRPAIL g t t g t t t t t t c a g c t g a g g a c c c c c g g t g c a g a t g g t t g g c c c c c g g c c c g c t a t t c t a a c a a c a a a a a a g t c g a c t c c t g g g g g c c a c g t c t a c c a a c c g g g g g c c g g g c g a t a a g a t t

2940 RKPFLILGRRCMRSDQIHGW g g a a g c c t t t c t t a a t t t t a g g g c g g c g c t g c a t g c g c t c a g a t c a g a t t c a c g g g t g g a c c t t c g g a a a g a a t t a a a a t c c c g c c g c g a c g t a c g c g a g t c t a g t c t a a g t g c c c a c c t

3000 RGLFSLFYFFLPAHIWACVN g a g g g t t a t t t t c t c t t t t t t a t t t t t t c t t g c c t g c t c a t a t t t g g g c c t g t g t g a a c c c t c c c a a t a a a a g a g a a a a a a t a a a a a a g a a c g g a c g a g t a t a a a c c c g g a c a c a c t t g g

3060 PRLRLGPQEPLLFKGDPRLQ c c a g g c t c c g c c t c g g c c c c c a g g a g c c g c t c t t a t t t a a a g g g g a t c c t c g c c t t c a g t g g t c c g a g g c g g a g c c g g g g g t c c t c g g c g a g a a t a a a t t t c c c c t a g g a g c g g a a g t c a

AfeI 3120 SSSQWKHQSCDNNLVSPTDQ c t t c g t c c c a g t g g a a g c a t c a g t c t t g t g a t a a c a a c t t g g t g a g t c c t a c a g a c c a g c g a a g c a g g g t c a c c t t c g t a g t c a g a a c a c t a t t g t t g a a c c a c t c a g g a t g t c t g g t c g

3180 RSTLGFKCIDFSGLATGRVL g c t c g a c t c t a g g c t t t a a a t g c a t t g a t t t c t c t g g a t t a g c t a c c g g g a g a g t t t t a a c g a g c t g a g a t c c g a a a t t t a c g t a a c t a a a g a g a c c t a a t c g a t g g c c c t c t c a a a a t t

3240 TLLISLVLELLGLKRVFSNA c t t t g c t t a t t a g t c t t g t g c t t g a a c t g c t a g g t t t a a a g a g g g t t t t c t c t a a t g c a t g a a a c g a a t a a t c a g a a c a c g a a c t t g a c g a t c c a a a t t t c t c c c a a a a g a g a t t a c g t a

3300 CMKRQLKTRNRAFVLLLNSS g c a t g a a g c g a c a g c t g a a a a c c a g a a a t a g a g c g t t t g t c c t c c t g t t a a a c t c t a g c c c g t a c t t c g c t g t c g a c t t t t g g t c t t t a t c t c g c a a a c a g g a g g a c a a t t t g a g a t c g g

NgoMIVNaeI 3360 LSLAFVSPSLQLPAGDWVSV t t t c t t t g g c a t t t g t c a g t c c g a g t c t t c a g c t g c c g g c a g g a g a t t g g g t t t c a g t a a a a a g a a a c c g t a a a c a g t c a g g c t c a g a a g t c g a c g g c c g t c c t c t a a c c c a a a g t c a t t

3420 TLAEHKLHVLPSDLIHY* c a c t t g c t g a g c a c a a a c t a c a c g t g c t t c c t t c a g a t t t a a t t c a t t a t t a a t c a t t c t g t g a a c g a c t c g t g t t t g a t g t g c a c g a a g g a a g t c t a a a t t a a g t a a t a a t t a g t a a g a

3480 a g a t t t a a a t g a t c t t c t t a c t c a t t g c t g a t t a t t t t a g c c t t g c g g a g c a g a t t a a a t t c t a a a t t t a c t a g a a g a a t g a g t a a c g a c t a a t a a a a t c g g a a c g c c t c g t c t a a t t t a

3540 g a g c a c g t g t t t a t t a a g a t t g a t t t a a a t a t a a t t a a t a t t t c a c c a c t g t a g t t t t a a c t c g t g c a c a a a t a a t t c t a a c t a a a t t t a t a t t a a t t a t a a a g t g g t g a c a t c a a a a t t

3600 a a t t g c c t t c t a a g t c a t g g t t g a t t t t t a a t a g g t a t c g g c t c t a a t t a g a t t a c t a t t t t a a c g g a a g a t t c a g t a c c a a c t a a a a a t t a t c c a t a g c c g a g a t t a a t c t a a t g a t a a

3660 t t g g t t t t t a a a t g t t a t t t c t a t t t c a t t t g t a g g c t c g t t t t t a a t g t g a t t t t a t t a a a c c a a a a a t t t a c a a t a a a g a t a a a g t a a a c a t c c g a g c a a a a a t t a c a c t a a a a t a a t

3720 g a t t c t a g t t t t a a a a t c a t c t t t t a g c c t t a c t g a g c a g a c t g a a t g a a c a t g t t t c a t c t a a g a t c a a a a t t t t a g t a g a a a a t c g g a a t g a c t c g t c t g a c t t a c t t g t a c a a a g t a

3780 t a a g a t t a a c t t c a t t a t a g t t a a t t a t t t a a t c a t t c t a g t g g a t t a t a a a t c a g c t c t a t t c t a a t t g a a g t a a t a t c a a t t a a t a a a t t a g t a a g a t c a c c t a a t a t t t a g t c g a g a

3840 a t t t a a a t t g a t t g t c g t g t t t a t t t c t a t t t g a t t t t t g a t t a t t t t t t a a g g t c g t t t t a a a t t t a a c t a a c a g c a c a a a t a a a g a t a a a c t a a a a a c t a a t a a a a a a t t c c a g c a a a

3900 t t a t g t g a t t t t t c c c c g a t t c t a a t t t t a a a c t a a t t g t t t g a t t t t t a t a t g a t t c g a a a t a c a c t a a a a a g g g g c t a a g a t t a a a a t t t g a t t a a c a a a c t a a a a a t a t a c t a a g c t

3960 c t c t t t t t a a a t g a a t t g t t t t g c t t t t t t t a t t g a g c a t a t a t a t a t a t t a t a c a a t t t g a g a a a a a t t t a c t t a a c a a a a c g a a a a a a a t a a c t c g t a t a t a t a t a t a a t a t g t t a a a

4020 c a a t c a a c a a t t g t t g t g c t t t t t a a a t g t t g t t t c t a t t t c a t t t c t a g g g t c g a t t t t g t t a g t t g t t a a c a a c a c g a a a a a t t t a c a a c a a a g a t a a a g t a a a g a t c c c a g c t a a a a

4080 a t t c g a t t c t a g t t t t a a a a t c g t g a t t t c t a a c t c c t a a c c t t a c t g a g c a g a c t g a a t t a a g c t a a g a t c a a a a t t t t a g c a c t a a a g a t t g a g g a t t g g a a t g a c t c g t c t g a c t t a

4140 g a a c a t g t t t c a t c a a g a t t a a c t t c a t t a t a g t t a a t t a t t g a a t c g g g c t a g t g g t t t c t t g t a c a a a g t a g t t c t a a t t g a a g t a a t a t c a a t t a a t a a c t t a g c c c g a t c a c c a a a

4200 a t a a a t c a g c t c t a t t a t a a a t t a a t t g t c a t c a t t a t t t c t a t t t a a t t t t t a t t a t t t t a t t t a g t c g a g a t a a t a t t t a a t t a a c a g t a g t a a t a a a g a t a a a t t a a a a a t a a t a a a

4260 t t t t a g g a t t a t t t t t a a g g g a t t t t t t t c a c g t t c t a g t t t t a a a c t c a a t g c t c a t t a a a a a t c c t a a t a a a a a t t c c c t a a a a a a a g t g c a a g a t c a a a a t t t g a g t t a c g a g t a a t

PacI 4320 c t g c t c a c c c t t g c t g a g c a g t c t t t c g t t a a g a t t a a a t t a a t t a a t a a c c c a t t c t a g g a c g a g t g g g a a c g a c t c g t c a g a a a g c a a t t c t a a t t t a a t t a a t t a t t g g g t a a g a t c

4380 t t t t a a a a c a g t g t c t t a a g g c a t a g c t g a t a t t t t a c a a g t t t a c t a t t t a a t a t a t c t a a a a t t t t g t c a c a g a a t t c c g t a t c g a c t a t a a a a t g t t c a a a t g a t a a a t t a t a t a g a

4440 c c a t t t c t t t t t t t a g g g t c g a t t t c a t c t g a t t t g a t t a a t t t t t a t t a t c t t c a c t a a g g t a a a g a a a a a a a t c c c a g c t a a a g t a g a c t a a a c t a a t t a a a a a t a a t a g a a g t g a t t

4500 g c t t t t t g t t c t a g g t t t t a t t c t c a g t a t t a t t a c t g t t a t a t t t g a a c t g a a t t t c t c c g a a a a a c a a g a t c c a a a a t a a g a g t c a t a a t a a t g a c a a t a t a a a c t t g a c t t a a a g a g

4560 c t c a t a g t t t a t t c a t t t t a t t t a t t t a t t t t t t g t t t a t t c g c t a t t t t g t a t t t t a g t g a g t a t c a a a t a a g t a a a a t a a a t a a a t a a a a a a c a a a t a a g c g a t a a a a c a t a a a a t c a

4620 t t t t a a t t a g a a a t t t t a t t a g a a t c g t t t t g a a c t a g t t t t t a t g c a c t c t a a c a t t a a a a a a t t a a t c t t t a a a a t a a t c t t a g c a a a a c t t g a t c a a a a a t a c g t g a g a t t g t a a t t

4680 t a t t a a a t c a t a a c t a t t a t t t t t a t t a t a a t t t a t t t g t g t t t a t c t t t t t g t g t a g c t a t a a t t t a g t a t t g a t a a t a a a a a t a a t a t t a a a t a a a c a c a a a t a g a a a a a c a c a t c g a

4740 t t c a t g t g t t t t t a t t t t a g t t t t a t t a t g t t t g c a t t g a a t t t t t t g g a c t a g t t t a t a a a g t a c a c a a a a a t a a a a t c a a a a t a a t a c a a a c g t a a c t t a a a a a a c c t g a t c a a a t a t

4800 t a a c g a t a t a c a g a a a a g c c a t g a c a a c a a a t g t t t t t a t a a t a c c t a t t a a g t a t a a g c a t t g c t a t a t g t c t t t t c g g t a c t g t t g t t t a c a a a a a t a t t a t g g a t a a t t c a t a t t c g

4860 t a a t c a g a t t t c c a c t a t t c t g t t c a a a g t t a a c a t t a t t a g c c a t t c t g a t g g a t c a t t a t t a g t c t a a a g g t g a t a a g a c a a g t t t c a a t t g t a a t a a t c g g t a a g a c t a c c t a g t a a

4920 t g a c a t t t g t c a t a t g t c t t t a t g a a c a c t g c t c t t t c a g a t g c c a c t t t t a a a g c t g t t a c t g t a a a c a g t a t a c a g a a a t a c t t g t g a c g a g a a a g t c t a c g g t g a a a a t t t c g a c a a

Bsu36I 4980 t t t t a c a g t a a a t g a a c g t t t a t t a t t a t g a c a t t a t t t c c t a a g g c c t c t a g a g t a c a g a a a a t g t c a t t t a c t t g c a a a t a a t a a t a c t g t a a t a a a g g a t t c c g g a g a t c t c a t g t c

5040 c a g g a t g t g g c g t g t g c a t t a c t g t g t a a t t a t g c t g a c t g t a a a t g t g c a t t t a t a g a c g t c c t a c a c c g c a c a c g t a a t g a c a c a t t a a t a c g a c t g a c a t t t a c a c g t a a a t a t c t g

5100 attB1 t g t c a g c a t g t c t g a c t g t g c g c t g t t g t g a t t t g t c c t g c a c a g a c c a a g g c a a g t t t g a c a g t c g t a c a g a c t g a c a c g c g a c a a c a c t a a a c a g g a c g t g t c t g g t t c c g t t c a a a c

NcoI 5160 1 5 10 MVSELIKENMHMK attB1 mKate2 Kozak sequence MVSELIKENMHMK t a c a a a a a a g c a g g c t g c c a c c a t g g t g a g c g a g c t g a t t a a g g a g a a c a t g c a c a t g a a a t g t t t t t t c g t c c g a c g g t g g t a c c a c t c g c t c g a c t a a t t c c t c t t g t a c g t g t a c t t

5220 15 20 25 30 LYMEGTVNNHHFKCTSEGEG mKate2 LYMEGTVNNHHFKCTSEGEG g c t g t a c a t g g a g g g c a c c g t g a a c a a c c a c c a c t t c a a g t g c a c a t c c g a g g g c g a a g g c g a c a t g t a c c t c c c g t g g c a c t t g t t g g t g g t g a a g t t c a c g t g t a g g c t c c c g c t t c c

5280 35 40 45 50 KPYEGTQTMRIKAVEGGPLP mKate2 KPYEGTQTMRIKAVEGGPLP c a a g c c c t a c g a g g g c a c c c a g a c c a t g a g a a t c a a g g c g g t c g a g g g c g g c c c t c t c c c g t t c g g g a t g c t c c c g t g g g t c t g g t a c t c t t a g t t c c g c c a g c t c c c g c c g g g a g a g g g

5340 55 60 65 70 FAFDILATSFMYGSKTFINH mKate2 FAFDILATSFMYGSKTFINH c t t c g c c t t c g a c a t c c t g g c t a c c a g c t t c a t g t a c g g c a g c a a a a c c t t c a t c a a c c a g a a g c g g a a g c t g t a g g a c c g a t g g t c g a a g t a c a t g c c g t c g t t t t g g a a g t a g t t g g t

PasI 5400 75 80 85 90 TQGIPDFFKQSFPEGFTWER mKate2 TQGIPDFFKQSFPEGFTWER c a c c c a g g g c a t c c c c g a c t t c t t t a a g c a g t c c t t c c c c g a g g g c t t c a c a t g g g a g a g g t g g g t c c c g t a g g g g c t g a a g a a a t t c g t c a g g a a g g g g c t c c c g a a g t g t a c c c t c t c

5460 95 100 105 110 VTTYEDGGVLTATQDTSLQD mKate2 VTTYEDGGVLTATQDTSLQD a g t c a c c a c a t a c g a a g a c g g g g g c g t g c t g a c c g c t a c c c a g g a c a c c a g c c t c c a g g a t c a g t g g t g t a t g c t t c t g c c c c c g c a c g a c t g g c g a t g g g t c c t g t g g t c g g a g g t c c t

5520 115 120 125 130 GCLIYNVKIRGVNFPSNGPV mKate2 GCLIYNVKIRGVNFPSNGPV c g g c t g c c t c a t c t a c a a c g t c a a g a t c a g a g g g g t g a a c t t c c c a t c c a a c g g c c c t g t g c c g a c g g a g t a g a t g t t g c a g t t c t a g t c t c c c c a c t t g a a g g g t a g g t t g c c g g g a c a

5580 135 140 145 150 MQKKTLGWEASTETLYPADG mKate2 MQKKTLGWEASTETLYPADG g a t g c a g a a g a a a a c a c t c g g c t g g g a g g c c t c c a c c g a g a c c c t g t a c c c c g c t g a c g g c t a c g t c t t c t t t t g t g a g c c g a c c c t c c g g a g g t g g c t c t g g g a c a t g g g g c g a c t g c c

5640 155 160 165 170 GLEGRADMALKLVGGGHLIC mKate2 GLEGRADMALKLVGGGHLIC c g g c c t g g a a g g c a g a g c c g a c a t g g c c c t g a a g c t c g t g g g c g g g g g c c a c c t g a t c t g g c c g g a c c t t c c g t c t c g g c t g t a c c g g g a c t t c g a g c a c c c g c c c c c g g t g g a c t a g a c

5700 175 180 185 190 NLKTTYRSKKPAKNLKMPGV mKate2 NLKTTYRSKKPAKNLKMPGV c a a c t t g a a g a c c a c a t a c a g a t c c a a g a a a c c c g c t a a g a a c c t c a a g a t g c c c g g c g t g t t g a a c t t c t g g t g t a t g t c t a g g t t c t t t g g g c g a t t c t t g g a g t t c t a c g g g c c g c a

5760 195 200 205 210 YYVDRRLERIKEADKETYVE mKate2 YYVDRRLERIKEADKETYVE c t a c t a t g t g g a c a g a a g a c t g g a a a g a a t c a a g g a g g c c g a c a a a g a g a c c t a c g t c g a g a t g a t a c a c c t g t c t t c t g a c c t t t c t t a g t t c c t c c g g c t g t t t c t c t g g a t g c a g c t

DraIII 5820 215 220 225 230 QHEVAVARYCDLPSKLGHR D mKate2 attB2 QHEVAVARYCDLPSKLGHRD g c a g c a c g a g g t g g c t g t g g c c a g a t a c t g c g a c c t c c c t a g c a a a c t g g g g c a c a g a g a c g t c g t g c t c c a c c g a c a c c g g t c t a t g a c g c t g g a g g g a t c g t t t g a c c c c g t g t c t c t

5880 (in frame with mKate2) PAFLYKVVDTLWQYQFRIIL attB2 PAFLYKVVDTLWQYQFRIIL c c c a g c t t t c t t g t a c a a a g t g g t g g a t a c t t t a t g g c a g t a c c a g t t c a g g a t c a t t t t g g g t c g a a a g a a c a t g t t t c a c c a c c t a t g a a a t a c c g t c a t g g t c a a g t c c t a g t a a a a

5940 (in frame with mKate2) LGDSTVGKSSLLKRFTDGIY LGDSTVGKSSLLKRFTDGIY a c t c g g g g a c t c g a c g g t g g g c a a a t c a t c t t t g c t c a a g a g g t t c a c g g a t g g c a t a t a t g a g c c c c t g a g c t g c c a c c c g t t t a g t a g a a a c g a g t t c t c c a a g t g c c t a c c g t a t a t

6000 (in frame with mKate2) SDVADPTVGVDFYARSIDIE SDVADPTVGVDFYARSIDIE c a g c g a t g t a g c g g a t c c g a c g g t c g g t g t g g a t t t c t a c g c c c g t t c a a t a g a c a t c g a g t c g c t a c a t c g c c t a g g c t g c c a g c c a c a c c t a a a g a t g c g g g c a a g t t a t c t g t a g c t

6060 (in frame with mKate2) PGVKIKLQLWDTAGQERFRS PGVKIKLQLWDTAGQERFRS a c c t g g g g t t a a a a t c a a a c t a c a g t t a t g g g a t a c a g c c g g a c a g g a g a g a t t c a g g t c t g g a c c c c a a t t t t a g t t t g a t g t c a a t a c c c t a t g t c g g c c t g t c c t c t c t a a g t c c a g

6120 (in frame with mKate2) ITTSYYRNSVGGLLVFDLTN ITTSYYRNSVGGLLVFDLTN t a t c a c c a c t t c c t a c t a t c g c a a c t c t g t g g g c g g t c t c t t a g t t t t t g a c c t g a c g a a a t a g t g g t g a a g g a t g a t a g c g t t g a g a c a c c c g c c a g a g a a t c a a a a a c t g g a c t g c t t

6180 (in frame with mKate2) RKTFDHVREWHREVSEHILP RKTFDHVREWHREVSEHILP c a g g a a g a c c t t t g a t c a t g t g c g g g a a t g g c a c c g a g a a g t c a g c g a g c a c a t c c t g c c g t c c t t c t g g a a a c t a g t a c a c g c c c t t a c c g t g g c t c t t c a g t c g c t c g t g t a g g a c g g

AsiSI 6240 (in frame with mKate2) HHMVYILIGHKSDLSRDRKV HHMVYILIGHKSDLSRDRKV t c a t c a c a t g g t c t a c a t c c t g a t c g g c c a c a a g a g c g a c c t g a g c c g c g a t c g c a a g g t a g t a g t g t a c c a g a t g t a g g a c t a g c c g g t g t t c t c g c t g g a c t c g g c g c t a g c g t t c c a

BmtINheI 6300 (in frame with mKate2) TRDEAEQLAADLGIRYVETS TRDEAEQLAADLGIRYVETS g a c a c g a g a t g a g g c c g a g c a g c t a g c g g c c g a t c t c g g a a t c c g c t a t g t g g a a a c a t c c t g t g c t c t a c t c c g g c t c g t c g a t c g c c g g c t a g a g c c t t a g g c g a t a c a c c t t t g t a g

6360 (in frame with mKate2) AKCNSNVERAFELLTRDIYE AKCNSNVERAFELLTRDIYE a g c c a a a t g c a a c a g c a a t g t g g a a c g t g c t t t c g a g c t g c t c a c a c g g g a c a t t t a t g a t c g g t t t a c g t t g t c g t t a c a c c t t g c a c g a a a g c t c g a c g a g t g t g c c c t g t a a a t a c t

6420 (in frame with mKate2) LMKMGEISTRDGWDGVKSGL LMKMGEISTRDGWDGVKSGL g c t g a t g a a g a t g g g t g a g a t c t c c a c t c g t g a c g g c t g g g a t g g g g t c a a g a g c g g c c t c g a c t a c t t c t a c c c a c t c t a g a g g t g a g c a c t g c c g a c c c t a c c c c a g t t c t c g c c g g a

6480 (in frame with mKate2) TAKVLYPAEEEAEREKSCNC TAKVLYPAEEEAEREKSCNC c a c t g c c a a a g t t c t t t a t c c a g c t g a g g a g g a a g c g g a g c g a g a a a a g a g c t g c a a c t g g t g a c g g t t t c a a g a a a t a g g t c g a c t c c t c c t t c g c c t c g c t c t t t t c t c g a c g t t g a c

Acc65IKpnI 6540 * attB3 M13 fwd * c t g a c a a c t t t a t t a t a c a t a g t t g a t a a t t c a c t g g c c g t c g t t t t a c g g t a c c a t c g a g a c t g t t g a a a t a a t a t g t a t c a a c t a t t a a g t g a c c g g c a g c a a a a t g c c a t g g t a g c t

6600 SV40 poly(A) signal C T A C T C A A A C C T G T T T G G T G EBV-rev t g a t g a t c c a g a c a t g a t a a g a t a c a t t g a t g a g t t t g g a c a a a c c a c a a c t a g a a t g c a a c t a c t a g g t c t g t a c t a t t c t a t g t a a c t a c t c a a a c c t g t t t g g t g t t g a t c t t a c g t

6660 SV40 poly(A) signal G A A A T T T G T G A T G C T A T T G C SV40pA-R g t g a a a a a a a t g c t t t a t t t g t g a a a t t t g t g a t g c t a t t g c t t t a t t t g t a a c c a t t a t c a c t t t t t t t a c g a a a t a a a c a c t t t a a a c a c t a c g a t a a c g a a a t a a a c a t t g g t a a t a

6720 SV40 poly(A) signal a a g c t g c a a t a a a c a a g t t a a c a a c a a c a a t t g c a t t c a t t t t a t g t t t c a g g t t c a g g g t t c g a c g t t a t t t g t t c a a t t g t t g t t g t t a a c g t a a g t a a a a t a c a a a g t c c a a g t c c c

6780 g g a g g t g t g g g a g g t t t t t t a a a g c a a g t a a a a c c t c t a c a a a t g t g g t a t g g c t g a t t a c c t c c a c a c c c t c c a a a a a a t t t c g t t c a t t t t g g a g a t g t t t a c a c c a t a c c g a c t a a t

6840 t g a t c c t c t a g a t c a g a t c t g c g a a g a t a c g g c c a c g g g t g c t c t t g a t c c t g t g g c t g a a c t a g g a g a t c t a g t c t a g a c g c t t c t a t g c c g g t g c c c a c g a g a a c t a g g a c a c c g a c t

6900 t t t t g g a c t g t g c t g c t c g c a g c t g c t g a t g a a t c a c a t a c t t c c t c c a t t t t c t t c c a c a a a a c c t g a c a c g a c g a g c g t c g a c g a c t a c t t a g t g t a t g a a g g a g g t a a a a g a a g g t g

6960 t g a t t g a c t g t t a t a a t t t c c c t a a t t t c c a g g t c a a g g t g c t g t g c a t t g t g g t a a t a g a c t a a c t g a c a a t a t t a a a g g g a t t a a a g g t c c a g t t c c a c g a c a c g t a a c a c c a t t a t c

ZraIAatII 7020 a t g t g a c a t g a c g t c a c t t c c a a a g g a c c a a t g a a c a t g t c t g a c c a a t t t c a t a t a a t g t a c a c t g t a c t g c a g t g a a g g t t t c c t g g t t a c t t g t a c a g a c t g g t t a a a g t a t a t t a c

7080 t g a a a a c g a t t t t c a t a g g c a g a a t a a a t a a c a t t t a a a t t a a a c t g g g c a t c a g c g c a a a c t t t t g c t a a a a g t a t c c g t c t t a t t t a t t g t a a a t t t a a t t t g a c c c g t a g t c g c g t t

7140 t t c a a t t g g t t t g g t a a t a g c a a g g g a a a a t a g a a t g a a g t g a t c t c c a a a a a a t a a g t a a a g t t a a c c a a a c c a t t a t c g t t c c c t t t t a t c t t a c t t c a c t a g a g g t t t t t t a t t c a t

7200 c t t t t t g a c t g t a a a t a a a a t t g t a a g g a g t a a a a a g t a c t t t t t t t t c t a a a a a a a t g t g a a a a a c t g a c a t t t a t t t t a a c a t t c c t c a t t t t t c a t g a a a a a a a a g a t t t t t t t a c a

7260 a a t t a a g t a a a a g t a a a a g t a t t g a t t t t t a a t t g t a c t c a a g t a a a g t a a a a a t c c c c a t t a a t t c a t t t t c a t t t t c a t a a c t a a a a a t t a a c a t g a g t t c a t t t c a t t t t t a g g g g t

7320 a a a a t a a t a c t t a a g t a c a g t a a t c a a g t a a a a t t a c t c a a g t a c t t t a c a c c t c t g g t t t t t t a t t a t g a a t t c a t g t c a t t a g t t c a t t t t a a t g a g t t c a t g a a a t g t g g a g a c c a a

NotI 7380 SK primer C T A G G T G A T C A A G A T C T pBluescriptSK c t t g a c c c c c t a c c t t c a g c a a g c c c a g c a g a t c c a c t a g t t c t a g a g c g g c c g c c a c c g g a a c t g g g g g a t g g a a g t c g t t c g g g t c g t c t a g g t g a t c a a g a t c t c g c c g g c g g t g g c

BssHIISacIIEco53kISacI 7440 T3 promoter G G A A A T C A C T C C C A A T T A A C G T3 c g g t g g a g c t c c a g c t t t t g t t c c c t t t a g t g a g g g t t a a t t g c g c g c t t g g c g t a a t c a g c c a c c t c g a g g t c g a a a a c a a g g g a a a t c a c t c c c a a t t a a c g c g c g a a c c g c a t t a g t

7500 -10 M13 rev lac operator lac promoter G G A C A C A C T T T A A C A A T A G G C G A M13/pUC Reverse t g g t c a t a g c t g t t t c c t g t g t g a a a t t g t t a t c c g c t c a c a a t t c c a c a c a a c a t a c g a a c c a g t a t c g a c a a a g g a c a c a c t t t a a c a a t a g g c g a g t g t t a a g g t g t g t t g t a t g c t

7560 -35 lac promoter CAP binding site g c c g g a a g c a t a a a g t g t a a a g c c t g g g g t g c c t a a t g a g t g a g c t a a c t c a c a t t a a t t c g g c c t t c g t a t t t c a c a t t t c g g a c c c c a c g g a t t a c t c a c t c g a t t g a g t g t a a t t a a

7620 g c g t t g c g c t c a c t g c c c g c t t t c c a g t c g g g a a a c c t g t c g t g c c a g c t g c a t t a a t g a c g c a a c g c g a g t g a c g g g c g a a a g g t c a g c c c t t t g g a c a g c a c g g t c g a c g t a a t t a c t

BspQISapI 7680 G A G C G A G L4440 a t c g g c c a a c g c g c g g g g a g a g g c g g t t t g c g t a t t g g g c g c t c t t c c g c t t c c t c g c t c t a g c c g g t t g c g c g c c c c t c t c c g c c a a a c g c a t a a c c c g c g a g a a g g c g a a g g a g c g a g

7740 T G A C T G A G C G A L4440 a c t g a c t c g c t g c g c t c g g t c g t t c g g c t g c g g c g a g c g g t a t c a g c t c a c t c a a a g g c g t g a c t g a g c g a c g c g a g c c a g c a a g c c g a c g c c g c t c g c c a t a g t c g a g t g a g t t t c c g c

7800 g t a a t a c g g t t a t c c a c a g a a t c a g g g g a t a a c g c a g g a a a g a a c a t g t g a g c a a a a g g c c a t t a t g c c a a t a g g t g t c t t a g t c c c c t a t t g c g t c c t t t c t t g t a c a c t c g t t t t c c g

7860 ori c a g c a a a a g g c c a g g a a c c g t a a a a a g g c c g c g t t g c t g g c g t t t t t c c a t a g g c t c c g c g t c g t t t t c c g g t c c t t g g c a t t t t t c c g g c g c a a c g a c c g c a a a a a g g t a t c c g a g g c g

7920 ori c c c c c t g a c g a g c a t c a c a a a a a t c g a c g c t c a a g t c a g a g g t g g c g a a a c c c g a c a g g a g g g g g a c t g c t c g t a g t g t t t t t a g c t g c g a g t t c a g t c t c c a c c g c t t t g g g c t g t c c t

7980 ori T T T C T A T G G T C C G C A A A G G G pBR322ori-F c t a t a a a g a t a c c a g g c g t t t c c c c c t g g a a g c t c c c t c g t g c g c t c t c c t g t t c c g a c c g a t a t t t c t a t g g t c c g c a a a g g g g g a c c t t c g a g g g a g c a c g c g a g a g g a c a a g g c t g g

8040 ori c t g c c g c t t a c c g g a t a c c t g t c c g c c t t t c t c c c t t c g g g a a g c g t g g c g c t t t c t c a t g a c g g c g a a t g g c c t a t g g a c a g g c g g a a a g a g g g a a g c c c t t c g c a c c g c g a a a g a g t a

8100 ori a g c t c a c g c t g t a g g t a t c t c a g t t c g g t g t a g g t c g t t c g c t c c a a g c t g g g c t g t g t g t c g a g t g c g a c a t c c a t a g a g t c a a g c c a c a t c c a g c a a g c g a g g t t c g a c c c g a c a c a c

8160 ori c a c g a a c c c c c c g t t c a g c c c g a c c g c t g c g c c t t a t c c g g t a a c t a t c g t c t t g a g t c c g t g c t t g g g g g g c a a g t c g g g c t g g c g a c g c g g a a t a g g c c a t t g a t a g c a g a a c t c a g g

8220 ori a a c c c g g t a a g a c a c g a c t t a t c g c c a c t g g c a g c a g c c a c t g g t a a c a g g a t t a g c a g a t t g g g c c a t t c t g t g c t g a a t a g c g g t g a c c g t c g t c g g t g a c c a t t g t c c t a a t c g t c t

8280 ori g c g a g g t a t g t a g g c g g t g c t a c a g a g t t c t t g a a g t g g t g g c c t a a c t a c g g c t a c a c t c g c t c c a t a c a t c c g c c a c g a t g t c t c a a g a a c t t c a c c a c c g g a t t g a t g c c g a t g t g a

8340 ori a g a a g g a c a g t a t t t g g t a t c t g c g c t c t g c t g a a g c c a g t t a c c t t c g g a a a a a g a g t t t c t t c c t g t c a t a a a c c a t a g a c g c g a g a c g a c t t c g g t c a a t g g a a g c c t t t t t c t c a a

8400 ori g g t a g c t c t t g a t c c g g c a a a c a a a c c a c c g c t g g t a g c g g t g g t t t t t t t g t t t g c a a g c c a t c g a g a a c t a g g c c g t t t g t t t g g t g g c g a c c a t c g c c a c c a a a a a a a c a a a c g t t c

8460 ori c a g c a g a t t a c g c g c a g a a a a a a a g g a t c t c a a g a a g a t c c t t t g a t c t t t t c t a c g g g g g t c g t c t a a t g c g c g t c t t t t t t t c c t a g a g t t c t t c t a g g a a a c t a g a a a a g a t g c c c c

8520 t c t g a c g c t c a g t g g a a c g a a a a c t c a c g t t a a g g g a t t t t g g t c a t g a g a t t a t c a a a a a g a c t g c g a g t c a c c t t g c t t t t g a g t g c a a t t c c c t a a a a c c a g t a c t c t a a t a g t t t t

8580 a g g a t c t t c a c c t a g a t c c t t t t a a a t t a a a a a t g a a g t t t t a a a t c a a t c t a a a g t a t a t c c t a g a a g t g g a t c t a g g a a a a t t t a a t t t t t a c t t c a a a a t t t a g t t a g a t t t c a t a t

8640 285 280 *WHKILSAGIEA AmpR *WHKILSAGIEA t a t g a g t a a a c t t g g t c t g a c a g t t a c c a a t g c t t a a t c a g t g a g g c a c c t a t c t c a g c g a t a c t c a t t t g a a c c a g a c t g t c a a t g g t t a c g a a t t a g t c a c t c c g t g g a t a g a g t c g c

8700 275 270 265 260 IQRNREDMTAQSGTTYIVVI AmpR IQRNREDMTAQSGTTYIVVI a t c t g t c t a t t t c g t t c a t c c a t a g t t g c c t g a c t c c c c g t c g t g t a g a t a a c t a c g a t a t a g a c a g a t a a a g c a a g t a g g t a t c a a c g g a c t g a g g g g c a g c a c a t c t a t t g a t g c t a t

8760 255 250 245 240 RSPKGDPGLAAIIGRSGREG AmpR RSPKGDPGLAAIIGRSGREG c g g g a g g g c t t a c c a t c t g g c c c c a g t g c t g c a a t g a t a c c g c g a g a c c c a c g c t c a c c g g c c c t c c c g a a t g g t a g a c c g g g g t c a c g a c g t t a c t a t g g c g c t c t g g g t g c g a g t g g c

8820 235 230 225 220 AGSKDAIFWGAPLASRLLPG AmpR AGSKDAIFWGAPLASRLLPG g c t c c a g a t t t a t c a g c a a t a a a c c a g c c a g c c g g a a g g g c c g a g c g c a g a a g t g g t c c t c g a g g t c t a a a t a g t c g t t a t t t g g t c g g t c g g c c t t c c c g g c t c g c g t c t t c a c c a g g a

8880 215 210 205 200 AVKDAEMWDILQQRSALTLL AmpR AVKDAEMWDILQQRSALTLL g c a a c t t t a t c c g c c t c c a t c c a g t c t a t t a a t t g t t g c c g g g a a g c t a g a g t a a g t a g t c g t t g a a a t a g g c g g a g g t a g g t c a g a t a a t t a a c a a c g g c c c t t c g a t c t c a t t c a t c a

FspI 8940 195 190 185 180 EGTLLKRLTTAMAVPMTTDR AmpR EGTLLKRLTTAMAVPMTTDR t c g c c a g t t a a t a g t t t g c g c a a c g t t g t t g c c a t t g c t a c a g g c a t c g t g g t g t c a c g c a g c g g t c a a t t a t c a a a c g c g t t g c a a c a a c g g t a a c g a t g t c c g t a g c a c c a c a g t g c g

9000 175 170 165 160 EDNPIAENLEPEWRDLRTVH AmpR EDNPIAENLEPEWRDLRTVH t c g t c g t t t g g t a t g g c t t c a t t c a g c t c c g g t t c c c a a c g a t c a a g g c g a g t t a c a t g a a g c a g c a a a c c a t a c c g a a g t a a g t c g a g g c c a a g g g t t g c t a g t t c c g c t c a a t g t a c t

9060 155 150 145 140 DGMNHLFATLEKPGGITTLL AmpR DGMNHLFATLEKPGGITTLL t c c c c c a t g t t g t g c a a a a a a g c g g t t a g c t c c t t c g g t c c t c c g a t c g t t g t c a g a a g t a g g g g g t a c a a c a c g t t t t t t c g c c a a t c g a g g a a g c c a g g a g g c t a g c a a c a g t c t t c a

9120 135 130 125 120 LNAATNDSMTIAASCLERVT AmpR LNAATNDSMTIAASCLERVT a a g t t g g c c g c a g t g t t a t c a c t c a t g g t t a t g g c a g c a c t g c a t a a t t c t c t t a c t g t c t t c a a c c g g c g t c a c a a t a g t g a g t a c c a a t a c c g t c g t g a c g t a t t a a g a g a a t g a c a g

9180 115 110 105 100 MGDTLHKETVPSYEVLDNQS AmpR MGDTLHKETVPSYEVLDNQS a t g c c a t c c g t a a g a t g c t t t t c t g t g a c t g g t g a g t a c t c a a c c a a g t c a t t c t g a g a a t a c g g t a g g c a t t c t a c g a a a a g a c a c t g a c c a c t c a t g a g t t g g t t c a g t a a g a c t c t t

9240 95 90 85 80 YHIRRGLQEQGADIRSLVAG AmpR A T A A T A C C G C G C C A Amp-R YHIRRGLQEQGADIRSLVAG t a g t g t a t g c g g c g a c c g a g t t g c t c t t g c c c g g c g t c a a t a c g g g a t a a t a c c g c g c c a a t c a c a t a c g c c g c t g g c t c a a c g a g a a c g g g c c g c a g t t a t g c c c t a t t a t g g c g c g g t

9300 75 70 65 60 CLLVKFTSMMPFREEPRFSE AmpR C A T A G C Amp-R CLLVKFTSMMPFREEPRFSE c a t a g c a g a a c t t t a a a a g t g c t c a t c a t t g g a a a a c g t t c t t c g g g g c g a a a a c t c t c a g t a t c g t c t t g a a a t t t t c a c g a g t a g t a a c c t t t t g c a a g a a g c c c c g c t t t t g a g a g t

9360 55 50 45 40 LIKGSNLDLEIYGVRAGLQD AmpR LIKGSNLDLEIYGVRAGLQD a g g a t c t t a c c g c t g t t g a g a t c c a g t t c g a t g t a a c c c a c t c g t g c a c c c a a c t g a t c t t c c t a g a a t g g c g a c a a c t c t a g g t c a a g c t a c a t t g g g t g a g c a c g t g g g t t g a c t a g a

9420 35 30 25 EADKVKVLTEPH 20 signal sequence AFVPLCFA AmpR EADKVKVLTEPHAFVPLCFA t c a g c a t c t t t t a c t t t c a c c a g c g t t t c t g g g t g a g c a a a a a c a g g a a g g c a a a a t g c c a g t c g t a g a a a a t g a a a g t g g t c g c a a a g a c c c a c t c g t t t t t g t c c t t c c g t t t t a c g g

9480 15 10 5 1 signal sequence AFFPILAVRFHQISM AmpR AmpR promoter AFFPILAVRFHQISM g c a a a a a a g g g a a t a a g g g c g a c a c g g a a a t g t t g a a t a c t c a t a c t c t t c c t t t t t c a a c g t t t t t t c c c t t a t t c c c g c t g t g c c t t t a c a a c t t a t g a g t a t g a g a a g g a a a a a g t t

9540 AmpR promoter t a t t a t t g a a g c a t t t a t c a g g g t t a t t g t c t c a t g a g c g g a t a c a t a t t t g a a t g t a t t a t a a t a a c t t c g t a a a t a g t c c c a a t a a c a g a g t a c t c g c c t a t g t a t a a a c t t a c a t a a

3ʹ 5ʹ 9588 AmpR promoter t a g a a a a a t a a a c a a a t a g g g g t t c c g c g c a c a t t t c c c c g a a a a g t g a t c t t t t t a t t t g t t t a t c c c c a a g g c g c g t g t a a a g g g g c t t t t c a c

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.