Addgene pBW2656_pCAG-PV1-nMag-L1-CTD-ENTRY-BGHpA - Sequence Analyzer Skip to main content
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Sequence Analyzer: pBW2656_pCAG-PV1-nMag-L1-CTD-ENTRY-BGHpA


Map View

PstI (5741) PflMI (5487) rbglobpA-R (5402 .. 5421) β-globin poly(A) signal Bglob-pA-R (5283 .. 5302) BglII (5280) BstXI (5259) MscI (5251) Bsu36I (5210) BmtI (5202) NheI (5198) NotI (5151) PvuII (5065) SphI (5019) BbsI (5008) EcoRI (4813) KpnI (4803) Acc65I (4799) BspDI - ClaI (4729) EcoRV (4352) NsiI (4314) MluI (4295) AscI (4246) pCAG-F (4183 .. 4202) XbaI (4128) pCAGGS-5 (4099 .. 4121) MreI - SgrAI (3813) BsiWI (3687) BlpI (3655) PfoI (3350) chicken β-actin promoter HindIII (4) M13 Reverse (22 .. 38) lac operator M13/pUC Reverse (35 .. 57) lac promoter BsaBI * (194) SfiI (307) StuI (353) AvrII (354) PsiI (394) SV40pA-R (412 .. 431) SV40 poly(A) signal EBV-rev (466 .. 485) BspQI - SapI (571) L4440 (577 .. 594) DrdI (795) pBR322ori-F (828 .. 847) AhdI (1580) BsaI (1641) PvuI (1950) ScaI (2060) Amp-R (2130 .. 2149) XmnI (2179) SspI (2384) SalI (2500) HincII (2502) SpeI (2517) SnaBI (2858) pBW2656_pCAG-PV1-nMag-L1-CTD-ENTRY-BGHpA 5742 bp

Sequence View

HindIII 60 M13 rev lac operator C A G T A T C G A C A A A G G A C M13 Reverse G G A C A C A C T T T A A C A A T A G G C G A M13/pUC Reverse c c c a a g c t t g g c g t a a t c a t g g t c a t a g c t g t t t c c t g t g t g a a a t t g t t a t c c g c t c a c g g g t t c g a a c c g c a t t a g t a c c a g t a t c g a c a a a g g a c a c a c t t t a a c a a t a g g c g a g t g

120 -10 -35 lac operator lac promoter CAP binding site a a t t c c a c a c a a c a t a c g a g c c g g a a g c a t a a a g t g t a a a g c c t g g g g t g c c t a a t g a g t t t a a g g t g t g t t g t a t g c t c g g c c t t c g t a t t t c a c a t t t c g g a c c c c a c g g a t t a c t c a

180 CAP binding site g a g c t a a c t c a c a t t a a t t g c g t t g c g c t c a c t g c c c g c t t t c c a g t c g g g a a a c c t g t c c t c g a t t g a g t g t a a t t a a c g c a a c g c g a g t g a c g g g c g a a a g g t c a g c c c t t t g g a c a g

BsaBI* 240 SV40 ori g t g c c a g c g g a t c c g c a t c t c a a t t a g t c a g c a a c c a t a g t c c c g c c c c t a a c t c c g c c c c a c g g t c g c c t a g g c g t a g a g t t a a t c a g t c g t t g g t a t c a g g g c g g g g a t t g a g g c g g g

300 SV40 ori a g t t c c g c c c a t t c t c c g c c c c a t g g c t g a c t a a t t t t t t t t a t t t a t g c a g a g g c c g a g t c a a g g c g g g t a a g a g g c g g g g t a c c g a c t g a t t a a a a a a a a t a a a t a c g t c t c c g g c t c

StuIAvrIISfiI 360 SV40 ori g c c g c c t c g g c c t c t g a g c t a t t c c a g a a g t a g t g a g g a g g c t t t t t t g g a g g c c t a g g c c g g c g g a g c c g g a g a c t c g a t a a g g t c t t c a t c a c t c c t c c g a a a a a a c c t c c g g a t c c g

PsiI 420 SV40 poly(A) signal C G T T A T C G T SV40pA-R t t t t g c a a a a a g c t a a c t t g t t t a t t g c a g c t t a t a a t g g t t a c a a a t a a a g c a a t a g c a a a a a c g t t t t t c g a t t g a a c a a a t a a c g t c g a a t a t t a c c a a t g t t t a t t t c g t t a t c g t

480 SV40 poly(A) signal G T G G T T T G T C C A A A C EBV-rev A G T G T T T A A A G SV40pA-R t c a c a a a t t t c a c a a a t a a a g c a t t t t t t t c a c t g c a t t c t a g t t g t g g t t t g t c c a a a c a g t g t t t a a a g t g t t t a t t t c g t a a a a a a a g t g a c g t a a g a t c a a c a c c a a a c a g g t t t g

540 SV40 poly(A) signal T C A T C EBV-rev t c a t c a a t g t a t c t t a t c a t g t c t g g a t c c g c t g c a t t a a t g a a t c g g c c a a c g c g c g g g a g t a g t t a c a t a g a a t a g t a c a g a c c t a g g c g a c g t a a t t a c t t a g c c g g t t g c g c g c c c

BspQISapI 600 G A G C G A G T G A C T G A G C G A L4440 g a g a g g c g g t t t g c g t a t t g g g c g c t c t t c c g c t t c c t c g c t c a c t g a c t c g c t g c g c t c c t c t c c g c c a a a c g c a t a a c c c g c g a g a a g g c g a a g g a g c g a g t g a c t g a g c g a c g c g a g

660 g g t c g t t c g g c t g c g g c g a g c g g t a t c a g c t c a c t c a a a g g c g g t a a t a c g g t t a t c c a c c c a g c a a g c c g a c g c c g c t c g c c a t a g t c g a g t g a g t t t c c g c c a t t a t g c c a a t a g g t g

720 a g a a t c a g g g g a t a a c g c a g g a a a g a a c a t g t g a g c a a a a g g c c a g c a a a a g g c c a g g a a t c t t a g t c c c c t a t t g c g t c c t t t c t t g t a c a c t c g t t t t c c g g t c g t t t t c c g g t c c t t

780 ori c c g t a a a a a g g c c g c g t t g c t g g c g t t t t t c c a t a g g c t c c g c c c c c c t g a c g a g c a t c a g g c a t t t t t c c g g c g c a a c g a c c g c a a a a a g g t a t c c g a g g c g g g g g g a c t g c t c g t a g t

DrdI 840 ori T T T C T A T G G T C C G pBR322ori-F c a a a a a t c g a c g c t c a a g t c a g a g g t g g c g a a a c c c g a c a g g a c t a t a a a g a t a c c a g g c g t t t t t a g c t g c g a g t t c a g t c t c c a c c g c t t t g g g c t g t c c t g a t a t t t c t a t g g t c c g

900 ori C A A A G G G pBR322ori-F g t t t c c c c c t g g a a g c t c c c t c g t g c g c t c t c c t g t t c c g a c c c t g c c g c t t a c c g g a t a c a a a g g g g g a c c t t c g a g g g a g c a c g c g a g a g g a c a a g g c t g g g a c g g c g a a t g g c c t a t

960 ori c c t g t c c g c c t t t c t c c c t t c g g g a a g c g t g g c g c t t t c t c a t a g c t c a c g c t g t a g g t a g g a c a g g c g g a a a g a g g g a a g c c c t t c g c a c c g c g a a a g a g t a t c g a g t g c g a c a t c c a t

1020 ori t c t c a g t t c g g t g t a g g t c g t t c g c t c c a a g c t g g g c t g t g t g c a c g a a c c c c c c g t t c a a g a g t c a a g c c a c a t c c a g c a a g c g a g g t t c g a c c c g a c a c a c g t g c t t g g g g g g c a a g t

1080 ori g c c c g a c c g c t g c g c c t t a t c c g g t a a c t a t c g t c t t g a g t c c a a c c c g g t a a g a c a c g a c g g g c t g g c g a c g c g g a a t a g g c c a t t g a t a g c a g a a c t c a g g t t g g g c c a t t c t g t g c t

1140 ori c t t a t c g c c a c t g g c a g c a g c c a c t g g t a a c a g g a t t a g c a g a g c g a g g t a t g t a g g c g g g a a t a g c g g t g a c c g t c g t c g g t g a c c a t t g t c c t a a t c g t c t c g c t c c a t a c a t c c g c c

1200 ori t g c t a c a g a g t t c t t g a a g t g g t g g c c t a a c t a c g g c t a c a c t a g a a g a a c a g t a t t t g g a c g a t g t c t c a a g a a c t t c a c c a c c g g a t t g a t g c c g a t g t g a t c t t c t t g t c a t a a a c c

1260 ori t a t c t g c g c t c t g c t g a a g c c a g t t a c c t t c g g a a a a a g a g t t g g t a g c t c t t g a t c c g g a t a g a c g c g a g a c g a c t t c g g t c a a t g g a a g c c t t t t t c t c a a c c a t c g a g a a c t a g g c c

1320 ori c a a a c a a a c c a c c g c t g g t a g c g g t g g t t t t t t t g t t t g c a a g c a g c a g a t t a c g c g c a g g t t t g t t t g g t g g c g a c c a t c g c c a c c a a a a a a a c a a a c g t t c g t c g t c t a a t g c g c g t c

1380 ori a a a a a a a g g a t c t c a a g a a g a t c c t t t g a t c t t t t c t a c g g g g t c t g a c g c t c a g t g g a a t t t t t t t c c t a g a g t t c t t c t a g g a a a c t a g a a a a g a t g c c c c a g a c t g c g a g t c a c c t t

1440 c g a a a a c t c a c g t t a a g g g a t t t t g g t c a t g a g a t t a t c a a a a a g g a t c t t c a c c t a g a t g c t t t t g a g t g c a a t t c c c t a a a a c c a g t a c t c t a a t a g t t t t t c c t a g a a g t g g a t c t a

1500 c c t t t t a a a t t a a a a a t g a a g t t t t a a a t c a a t c t a a a g t a t a t a t g a g t a a a c t t g g t c g g a a a a t t t a a t t t t t a c t t c a a a a t t t a g t t a g a t t t c a t a t a t a c t c a t t t g a a c c a g

1560 285 280 275 270 *WHKILSAGIEAIQRNRE AmpR *WHKILSAGIEAIQRNRE t g a c a g t t a c c a a t g c t t a a t c a g t g a g g c a c c t a t c t c a g c g a t c t g t c t a t t t c g t t c a c t g t c a a t g g t t a c g a a t t a g t c a c t c c g t g g a t a g a g t c g c t a g a c a g a t a a a g c a a g

AhdI 1620 265 260 255 250 DMTAQSGTTYIVVIRSPKGD AmpR DMTAQSGTTYIVVIRSPKGD a t c c a t a g t t g c c t g a c t c c c c g t c g t g t a g a t a a c t a c g a t a c g g g a g g g c t t a c c a t c t a g g t a t c a a c g g a c t g a g g g g c a g c a c a t c t a t t g a t g c t a t g c c c t c c c g a a t g g t a g

BsaI 1680 245 240 235 230 PGLAAIIGRSGREGAGSKDA AmpR PGLAAIIGRSGREGAGSKDA t g g c c c c a g t g c t g c a a t g a t a c c g c g a g a c c c a c g c t c a c c g g c t c c a g a t t t a t c a g c a c c g g g g t c a c g a c g t t a c t a t g g c g c t c t g g g t g c g a g t g g c c g a g g t c t a a a t a g t c g

1740 225 220 215 210 IFWGAPLASRLLPGAVKDAE AmpR IFWGAPLASRLLPGAVKDAE a a t a a a c c a g c c a g c c g g a a g g g c c g a g c g c a g a a g t g g t c c t g c a a c t t t a t c c g c c t c t t a t t t g g t c g g t c g g c c t t c c c g g c t c g c g t c t t c a c c a g g a c g t t g a a a t a g g c g g a g

1800 205 200 195 190 MWDILQQRSALTLLEGTLLK AmpR MWDILQQRSALTLLEGTLLK c a t c c a g t c t a t t a a t t g t t g c c g g g a a g c t a g a g t a a g t a g t t c g c c a g t t a a t a g t t t g t a g g t c a g a t a a t t a a c a a c g g c c c t t c g a t c t c a t t c a t c a a g c g g t c a a t t a t c a a a

1860 185 180 175 170 RLTTAMAVPMTTDREDNPIA AmpR RLTTAMAVPMTTDREDNPIA g c g c a a c g t t g t t g c c a t t g c t a c a g g c a t c g t g g t g t c a c g c t c g t c g t t t g g t a t g g c c g c g t t g c a a c a a c g g t a a c g a t g t c c g t a g c a c c a c a g t g c g a g c a g c a a a c c a t a c c g

1920 165 160 155 150 ENLEPEWRDLRTVHDGMNHL AmpR ENLEPEWRDLRTVHDGMNHL t t c a t t c a g c t c c g g t t c c c a a c g a t c a a g g c g a g t t a c a t g a t c c c c c a t g t t g t g c a a a a g t a a g t c g a g g c c a a g g g t t g c t a g t t c c g c t c a a t g t a c t a g g g g g t a c a a c a c g t t

PvuI 1980 145 140 135 130 FATLEKPGGITTLLLNAATN AmpR FATLEKPGGITTLLLNAATN a a a a g c g g t t a g c t c c t t c g g t c c t c c g a t c g t t g t c a g a a g t a a g t t g g c c g c a g t g t t t t t t c g c c a a t c g a g g a a g c c a g g a g g c t a g c a a c a g t c t t c a t t c a a c c g g c g t c a c a a

2040 125 120 115 110 DSMTIAASCLERVTMGDTLH AmpR DSMTIAASCLERVTMGDTLH a t c a c t c a t g g t t a t g g c a g c a c t g c a t a a t t c t c t t a c t g t c a t g c c a t c c g t a a g a t g t a g t g a g t a c c a a t a c c g t c g t g a c g t a t t a a g a g a a t g a c a g t a c g g t a g g c a t t c t a c

ScaI 2100 105 100 95 90 KETVPSYEVLDNQSYHIRRG AmpR KETVPSYEVLDNQSYHIRRG c t t t t c t g t g a c t g g t g a g t a c t c a a c c a a g t c a t t c t g a g a a t a g t g t a t g c g g c g a c c g a a a a g a c a c t g a c c a c t c a t g a g t t g g t t c a g t a a g a c t c t t a t c a c a t a c g c c g c t g g

2160 85 80 75 70 LQEQGADIRSLVAGCLLVKF AmpR A T A A T A C C G C G C C A C A T A G C Amp-R LQEQGADIRSLVAGCLLVKF g a g t t g c t c t t g c c c g g c g t c a a t a c g g g a t a a t a c c g c g c c a c a t a g c a g a a c t t t a a a c t c a a c g a g a a c g g g c c g c a g t t a t g c c c t a t t a t g g c g c g g t g t a t c g t c t t g a a a t t t

XmnI 2220 65 60 55 50 TSMMPFREEPRFSELIKGSN AmpR TSMMPFREEPRFSELIKGSN a g t g c t c a t c a t t g g a a a a c g t t c t t c g g g g c g a a a a c t c t c a a g g a t c t t a c c g c t g t t t c a c g a g t a g t a a c c t t t t g c a a g a a g c c c c g c t t t t g a g a g t t c c t a g a a t g g c g a c a a

2280 45 40 35 30 LDLEIYGVRAGLQDEADKVK AmpR LDLEIYGVRAGLQDEADKVK g a g a t c c a g t t c g a t g t a a c c c a c t c g t g c a c c c a a c t g a t c t t c a g c a t c t t t t a c t t t c t c t a g g t c a a g c t a c a t t g g g t g a g c a c g t g g g t t g a c t a g a a g t c g t a g a a a a t g a a a

2340 25 VLTEPH 20 15 10 signal sequence AFVPLCFAAFFPIL AmpR VLTEPHAFVPLCFAAFFPIL c a c c a g c g t t t c t g g g t g a g c a a a a a c a g g a a g g c a a a a t g c c g c a a a a a a g g g a a t a a g g t g g t c g c a a a g a c c c a c t c g t t t t t g t c c t t c c g t t t t a c g g c g t t t t t t c c c t t a t t c

SspI 2400 5 1 signal sequence AVRFHQISM AmpR AmpR promoter AVRFHQISM g g c g a c a c g g a a a t g t t g a a t a c t c a t a c t c t t c c t t t t t c a a t a t t a t t g a a g c a t t t a c c g c t g t g c c t t t a c a a c t t a t g a g t a t g a g a a g g a a a a a g t t a t a a t a a c t t c g t a a a t

2460 AmpR promoter t c a g g g t t a t t g t c t c a t g a g c g g a t a c a t a t t t g a a t g t a t t t a g a a a a a t a a a c a a a t a g t c c c a a t a a c a g a g t a c t c g c c t a t g t a t a a a c t t a c a t a a a t c t t t t t a t t t g t t t a

SpeISalIHincII 2520 AmpR promoter CMV enhancer a g g g g t t c c g c g c a c a t t t c c c c g a a a a g t g c c a c c t g g g t c g a c a t t g a t t a t t g a c t a t c c c c a a g g c g c g t g t a a a g g g g c t t t t c a c g g t g g a c c c a g c t g t a a c t a a t a a c t g a t

2580 CMV enhancer g t t a t t a a t a g t a a t c a a t t a c g g g g t c a t t a g t t c a t a g c c c a t a t a t g g a g t t c c g c g c a a t a a t t a t c a t t a g t t a a t g c c c c a g t a a t c a a g t a t c g g g t a t a t a c c t c a a g g c g c

2640 CMV enhancer t t a c a t a a c t t a c g g t a a a t g g c c c g c c t g g c t g a c c g c c c a a c g a c c c c c g c c c a t t g a a a t g t a t t g a a t g c c a t t t a c c g g g c g g a c c g a c t g g c g g g t t g c t g g g g g c g g g t a a c t

2700 CMV enhancer c g t c a a t a a t g a c g t a t g t t c c c a t a g t a a c g c c a a t a g g g a c t t t c c a t t g a c g t c a a t g c a g t t a t t a c t g c a t a c a a g g g t a t c a t t g c g g t t a t c c c t g a a a g g t a a c t g c a g t t a

2760 CMV enhancer g g g t g g a g t a t t t a c g g t a a a c t g c c c a c t t g g c a g t a c a t c a a g t g t a t c a t a t g c c a a c c c a c c t c a t a a a t g c c a t t t g a c g g g t g a a c c g t c a t g t a g t t c a c a t a g t a t a c g g t t

2820 CMV enhancer g t a c g c c c c c t a t t g a c g t c a a t g a c g g t a a a t g g c c c g c c t g g c a t t a t g c c c a g t a c a c a t g c g g g g g a t a a c t g c a g t t a c t g c c a t t t a c c g g g c g g a c c g t a a t a c g g g t c a t g t

SnaBI 2880 CMV enhancer t g a c c t t a t g g g a c t t t c c t a c t t g g c a g t a c a t c t a c g t a t t a g t c a t c g c t a t t a c c a a c t g g a a t a c c c t g a a a g g a t g a a c c g t c a t g t a g a t g c a t a a t c a g t a g c g a t a a t g g t

2940 CMV enhancer chicken β- actin promoter t g g t c g a g g t g a g c c c c a c g t t c t g c t t c a c t c t c c c c a t c t c c c c c c c c t c c c c a c c c c a c c a g c t c c a c t c g g g g t g c a a g a c g a a g t g a g a g g g g t a g a g g g g g g g g a g g g g t g g g g

3000 chicken β- actin promoter c a a t t t t g t a t t t a t t t a t t t t t t a a t t a t t t t g t g c a g c g a t g g g g g c g g g g g g g g g g g g t t a a a a c a t a a a t a a a t a a a a a a t t a a t a a a a c a c g t c g c t a c c c c c g c c c c c c c c c c c

3060 chicken β- actin promoter g g g c g c g c g c c a g g c g g g g c g g g g c g g g g c g a g g g g c g g g g c g g g g c g a g g c g g a a a g g t c c c g c g c g c g g t c c g c c c c g c c c c g c c c c g c t c c c c g c c c c g c c c c g c t c c g c c t t t c c a

3120 chicken β- actin promoter g c g g c g g c a g c c a a t c a g a g c g g c g c g c t c c g a a a g t t t c c t t t t a t g g c g a g g c g g c g g c g c c g c c g t c g g t t a g t c t c g c c g c g c g a g g c t t t c a a a g g a a a a t a c c g c t c c g c c g c c

3180 chicken β- actin promoter chimeric intron c g g c g g c g g c c c t a t a a a a a g c g a a g c g c g c g g c g g g c g g g a g t c g c t g c g t t g c c t t c g g c c g c c g c c g g g a t a t t t t t c g c t t c g c g c g c c g c c c g c c c t c a g c g a c g c a a c g g a a g c

3240 chimeric intron c c c c g t g c c c c g c t c c g c g c c g c c t c g c g c c g c c c g c c c c g g c t c t g a c t g a c c g c g t t a g g g g c a c g g g g c g a g g c g c g g c g g a g c g c g g c g g g c g g g g c c g a g a c t g a c t g g c g c a a t

3300 chimeric intron c t c c c a c a g g t g a g c g g g c g g g a c g g c c c t t c t c c t c c g g g c t g t a a t t a g c g c t t g g t t g a g g g t g t c c a c t c g c c c g c c c t g c c g g g a a g a g g a g g c c c g a c a t t a a t c g c g a a c c a a

PfoI 3360 chimeric intron *HRSTEKRHSRSLRLPSRSP t a a t g a c g g c t c g t t t c t t t t c t g t g g c t g c g t g a a a g c c t t a a a g g g c t c c g g g a g g g c a t t a c t g c c g a g c a a a g a a a a g a c a c c g a c g c a c t t t c g g a a t t t c c c g a g g c c c t c c c g

3420 chimeric intron GKHPPSRSPPHTRTHTRPSR c c t t t g t g c g g g g g g g a g c g g c t c g g g g g g t g c g t g c g t g t g t g t g t g c g t g g g g a g c g c g g a a a c a c g c c c c c c c t c g c c g a g c c c c c c a c g c a c g c a c a c a c a c a c g c a c c c c t c g c g

3480 chimeric intron RTRSRAARRSHASRARRPAK c g c g t g c g g c t c c g c g c t g c c c g g c g g c t g t g a g c g c t g c g g g c g c g g c g c g g g g c t t t g g c g c a c g c c g a g g c g c g a c g g g c c g c c g a c a c t c g c g a c g c c c g c g c c g c g c c c c g a a a c

3540 chimeric intron HAGCHARPSRPRPRHGATRP t g c g c t c c g c a g t g t g c g c g a g g g g a g c g c g g c c g g g g g c g g t g c c c c g c g g t g c g g g g g a c g c g a g g c g t c a c a c g c g c t c c c c t c g c g c c g g c c c c c g c c a c g g g g c g c c a c g c c c c c

3600 chimeric intron PSRPSCLSRAPHTRPPTLLP g g g c t g c g a g g g g a a c a a a g g c t g c g t g c g g g g t g t g t g c g t g g g g g g g t g a g c a g g g g g c c c g a c g c t c c c c t t g t t t c c g a c g c a c g c c c c a c a c a c g c a c c c c c c c a c t c g t c c c c c

BlpI 3660 chimeric intron HPRPPRATVGGQVGRGSNSL t g t g g g c g c g g c g g t c g g g c t g t a a c c c c c c c c t g c a c c c c c c t c c c c g a g t t g c t g a g c a c a c c c g c g c c g c c a g c c c g a c a t t g g g g g g g g a c g t g g g g g g a g g g g c t c a a c g a c t c g

BsiWI 3720 chimeric intron VARSRTRPETRPTARPEGHR a c g g c c c g g c t t c g g g t g c g g g g c t c c g t a c g g g g c g t g g c g c g g g g c t c g c c g t g c c g g t g c c g g g c c g a a g c c c a c g c c c c g a g g c a t g c c c c g c a c c g c g c c c c g a g c g g c a c g g c c

3780 chimeric intron APPPPLHPHRAPRPRRPGPL g c g g g g g g t g g c g g c a g g t g g g g g t g c c g g g c g g g g c g g g g c c g c c t c g g g c c g g g g a g g c g c c c c c c a c c g c c g t c c a c c c c c a c g g c c c g c c c c g c c c c g g c g g a g c c c g g c c c c t c c

SgrAIMreI 3840 chimeric intron ARPLPAAAGPAGAATSARRA g c t c g g g g g a g g g g c g c g g c g g c c c c c g g a g c g c c g g c g g c t g t c g a g g c g c g g c g a g c c c g a g c c c c c t c c c c g c g c c g c c g g g g g c c t c g c g g c c g c c g a c a g c t c c g c g c c g c t c g g

3900 chimeric intron AAM g c a g c c a t t g c c t t t t a t g g t a a t c g t g c g a g a g g g c g c a g g g a c t t c c t t t g t c c c a a a c g t c g g t a a c g g a a a a t a c c a t t a g c a c g c t c t c c c g c g t c c c t g a a g g a a a c a g g g t t t

3960 chimeric intron t c t g t g c g g a g c c g a a a t c t g g g a g g c g c c g c c g c a c c c c c t c t a g c g g g c g c g g g g c g a a g a c a c g c c t c g g c t t t a g a c c c t c c g c g g c g g c g t g g g g g a g a t c g c c c g c g c c c c g c t

4020 chimeric intron a g c g g t g c g g c g c c g g c a g g a a g g a a a t g g g c g g g g a g g g c c t t c g t g c g t c g c c g c g c c t c g c c a c g c c g c g g c c g t c c t t c c t t t a c c c g c c c c t c c c g g a a g c a c g c a g c g g c g c g g

4080 chimeric intron g c c g t c c c c t t c t c c c t c t c c a g c c t c g g g g c t g t c c g c g g g g g g a c g g c t g c c t t c g g g c g g c a g g g g a a g a g g g a g a g g t c g g a g c c c c g a c a g g c g c c c c c c t g c c g a c g g a a g c c c

XbaI 4140 chimeric intron G G T T C G G C T T C T G G C G T G T G A C C pCAGGS-5 g g g g a c g g g g c a g g g c g g g g t t c g g c t t c t g g c g t g t g a c c g g c g g c t c t a g a g c c t c t g c c c c t g c c c c g t c c c g c c c c a a g c c g a a g a c c g c a c a c t g g c c g c c g a g a t c t c g g a g a c

4200 chimeric intron G C A A C G T G C T G G T T A T T G pCAG-F c t a a c c a t g t t c a t g c c t t c t t c t t t t t c c t a c a g c t c c t g g g c a a c g t g c t g g t t a t t g g a t t g g t a c a a g t a c g g a a g a a g a a a a a g g a t g t c g a g g a c c c g t t g c a c g a c c a a t a a c

AscI 4260 T G pCAG-F t g c t g t c t c a t c a t t t t g g c a a a g a a t t g a t t a a t t c g a g c g a a g g c g c g c c c a t t a c t c a c g a c a g a g t a g t a a a a c c g t t t c t t a a c t a a t t a a g c t c g c t t c c g c g c g g g t a a t g a g

NsiIMluI 4320 MHTL g c a t c c a t t c t c a g g c t g t c t c g t c t c g t c t c g c a c g c g t g c c g c c a c c a t g c a t a c t c t c g t a g g t a a g a g t c c g a c a g a g c a g a g c a g a g c g t g c g c a c g g c g g t g g t a c g t a t g a g a

EcoRV 4380 YAPGGYDIMGYLDQIGNRPN t t a t g c c c c c g g t g g a t a t g a c a t t a t g g g a t a t c t g g a c c a g a t c g g c a a c c g g c c a a a a a t a c g g g g g c c a c c t a t a c t g t a a t a c c c t a t a g a c c t g g t c t a g c c g t t g g c c g g t t t

4440 PQVELGPVDTSCALILCDLK c c c g c a g g t g g a a c t g g g c c c c g t g g a t a c a t c c t g c g c c t t g a t t c t t t g t g a c c t g a a g g g c g t c c a c c t t g a c c c g g g g c a c c t a t g t a g g a c g c g g a a c t a a g a a a c a c t g g a c t t

4500 QKDTPIVYASEAFLYMTGYS a c a g a a a g a c a c c c c g a t a g t t t a c g c g a g t g a a g c c t t c c t c t a c a t g a c a g g t t a c a g t g t c t t t c t g t g g g g c t a t c a a a t g c g c t c a c t t c g g a a g g a g a t g t a c t g t c c a a t g t c

4560 NAEVLGRNCRFLQSPDGMVK c a a c g c a g a g g t g c t g g g c c g g a a t t g c c g g t t t c t g c a a a g c c c t g a c g g c a t g g t g a a g t t g c g t c t c c a c g a c c c g g c c t t a a c g g c c a a a g a c g t t t c g g g a c t g c c g t a c c a c t t

4620 PKSTRKYVDSNTINTMRKAI g c c c a a g a g c a c c c g g a a g t a c g t g g a t a g t a a c a c a a t c a a t a c t a t g c g c a a g g c a a t c g g g t t c t c g t g g g c c t t c a t g c a c c t a t c a t t g t g t t a g t t a t g a t a c g c g t t c c g t t a

4680 DRNAEVQVEVVNFKKNGQRF c g a c a g g a a t g c c g a g g t g c a g g t t g a a g t a g t c a a t t t t a a a a a g a a t g g a c a g c g a t t g c t g t c c t t a c g g c t c c a c g t c c a a c t t c a t c a g t t a a a a t t t t t c t t a c c t g t c g c t a a

BspDIClaI 4740 VNFLTMIPVRDETGEYRYSM t g t t a a t t t c c t g a c t a t g a t a c c t g t t a g g g a c g a a a c a g g c g a g t a t c g a t a c t c t a t a c a a t t a a a g g a c t g a t a c t a t g g a c a a t c c c t g c t t t g t c c g c t c a t a g c t a t g a g a t a

Acc65I 4800 GFQCETESGGSGSGSSGGSG g g g a t t c c a g t g c g a a a c a g a a t c c g g a g g g t c t g g c t c c g g a t c a a g t g g t g g c a g c g g c c c t a a g g t c a c g c t t t g t c t t a g g c c t c c c a g a c c g a g g c c t a g t t c a c c a c c g t c g c c

EcoRIKpnI 4860 bGH poly(A) signal TKSTNSCAF* t a c c a a g t c t a c g a a t t c c t g t g c c t t c t a g t t g c c a g c c a t c t g t t g t t t g c c c c t c c c a t g g t t c a g a t g c t t a a g g a c a c g g a a g a t c a a c g g t c g g t a g a c a a c a a a c g g g g a g g g

4920 bGH poly(A) signal c c g t g c c t t c c t t g a c c c t g g a a g g t g c c a c t c c c a c t g t c c t t t c c t a a t a a a a t g a g g g g c a c g g a a g g a a c t g g g a c c t t c c a c g g t g a g g g t g a c a g g a a a g g a t t a t t t t a c t c c

4980 bGH poly(A) signal a a a t t g c a t c g c a t t g t c t g a g t a g g t g t c a t t c t a t t c t g g g g g g t g g g g t g g g g c a g g t t t a a c g t a g c g t a a c a g a c t c a t c c a c a g t a a g a t a a g a c c c c c c a c c c c a c c c c g t c c

SphIBbsI 5040 bGH poly(A) signal a c a g c a a g g g g g a g g a t t g g g a a g a c a a t a g c a g g c a t g c t g g g g a t g c g g t g g g c t c t a t g t c g t t c c c c c t c c t a a c c c t t c t g t t a t c g t c c g t a c g a c c c c t a c g c c a c c c g a g a t

PvuII 5100 bGH poly(A) signal t g g c t t c t g a g g c g g a a a g a a c c a g c t g g g g c t c t a g g g g g t a t c c c c a c g c g c c c t g t a a c c g a a g a c t c c g c c t t t c t t g g t c g a c c c c g a g a t c c c c c a t a g g g g t g c g c g g g a c a t

NotI 5160 g c g t g t a c c g c t g g g a g t t c g t a g a c g g a a a c a a a c g c a g a a t c c a a g c g c g g c c g c c c a c g c a c a t g g c g a c c c t c a a g c a t c t g c c t t t g t t t g c g t c t t a g g t t c g c g c c g g c g g g t

Bsu36IBmtINheI 5220 g g a t a c a t a g a t t a c c a c a a c t c c g a g c c c t t c c a c c g c t a g c g c a c t c c t c a g g t g c a g c c t a t g t a t c t a a t g g t g t t g a g g c t c g g g a a g g t g g c g a t c g c g t g a g g a g t c c a c g t c

BstXIMscI 5280 g c t g c c t a t c a g a a g g t g g t g g c t g g t g t g g c c a a t g c c c t g g c t c a c a a a t a c c a c t g a c g a c g g a t a g t c t t c c a c c a c c g a c c a c a c c g g t t a c g g g a c c g a g t g t t t a t g g t g a c t

BglII 5340 A G A A A A A G G G A G A C G G T T T T Bglob-pA-R g a t c t t t t t c c c t c t g c c a a a a a t t a t g g g g a c a t c a t g a a g c c c c t t g a g c a t c t g a c t c t a g a a a a a g g g a g a c g g t t t t t a a t a c c c c t g t a g t a c t t c g g g g a a c t c g t a g a c t g a

5400 β- globin poly(A) signal t c t g g c t a a t a a a g g a a a t t t a t t t t c a t t g c a a t a g t g t g t t g g a a t t t t t t g t g t c t c a g a c c g a t t a t t t c c t t t a a a t a a a a g t a a c g t t a t c a c a c a a c c t t a a a a a a c a c a g a g

5460 β- globin poly(A) signal G T G A G C C T T C C T G T A T A C C C rbglobpA-R t c a c t c g g a a g g a c a t a t g g g a g g g c a a a t c a t t t a a a a c a t c a g a a t g a g t a t t t g g t t a g t g a g c c t t c c t g t a t a c c c t c c c g t t t a g t a a a t t t t g t a g t c t t a c t c a t a a a c c a a

PflMI 5520 t a g a g t t t g g c a a c a t a t g c c c a t a t g c t g g c t g c c a t g a a c a a a g g t t g g c t a t a a a g a a t c t c a a a c c g t t g t a t a c g g g t a t a c g a c c g a c g g t a c t t g t t t c c a a c c g a t a t t t c t

5580 g g t c a t c a g t a t a t g a a a c a g c c c c c t g c t g t c c a t t c c t t a t t c c a t a g a a a a g c c t t g c c a g t a g t c a t a t a c t t t g t c g g g g g a c g a c a g g t a a g g a a t a a g g t a t c t t t t c g g a a c

5640 a c t t g a g g t t a g a t t t t t t t t a t a t t t t g t t t t g t g t t a t t t t t t t c t t t a a c a t c c c t a t g a a c t c c a a t c t a a a a a a a a t a t a a a a c a a a a c a c a a t a a a a a a a g a a a t t g t a g g g a t

5700 a a a t t t t c c t t a c a t g t t t t a c t a g c c a g a t t t t t c c t c c t c t c c t g a c t a c t c c c a g t c t t t a a a a g g a a t g t a c a a a a t g a t c g g t c t a a a a a g g a g g a g a g g a c t g a t g a g g g t c a g

PstI 5742 a t a g c t g t c c c t c t t c t c t t a t g g a g a t c c c t c g a c c t g c a g t a t c g a c a g g g a g a a g a g a a t a c c t c t a g g g a g c t g g a c g t c

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.