Addgene pEGFP Kind2 S181A Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pEGFP Kind2 S181A Sequencing Result


Map View

BsaI (6463) TK-pA-R (6328 .. 6347) BstBI (6156) Neo-F (6010 .. 6029) RsrII (5990) PflFI - Tth111I (5592) FspI (5576) MscI (5556) PluTI (5477) SfoI (5475) NarI (5474) KasI (5473) Neo-R (5400 .. 5419) BspDI * - ClaI * (5314) CsiI - SexAI * (5063) pBABE 3' (4977 .. 4997) F1ori-R (4715 .. 4734) DraIII (4590) F1ori-F (4503 .. 4524) EBV-rev (4329 .. 4348) SV40pA-R (4275 .. 4294) HpaI (4237) MfeI (4224) XbaI * (4128) NotI (4118) BsrGI (4105) EGFP-C (4049 .. 4070) EXFP-R (3702 .. 3721) EGFP-N (3441 .. 3462) BamHI (3386) PflMI (3367) pBR322ori-F (518 .. 537) PciI (673) AseI (738) CMV enhancer NdeI (965) SnaBI (1071) CMV-F (1250 .. 1270) NheI (1322) BmtI (1326) BlpI (1597) BbvCI (1615) EcoNI (1855) AccI (1991) BstZ17I (1992) BglII (2103) BstEII (3203) EcoRI (3276) PmlI (3309) pEGFP Kind2 S181A 6719 bp

Sequence View

5ʹ 3ʹ 60 ori a c c c c g t a g a a a a g a t c a a a g g a t c t t c t t g a g a t c c t t t t t t t c t g c g c g t a a t c t g c t t g g g g c a t c t t t t c t a g t t t c c t a g a a g a a c t c t a g g a a a a a a a g a c g c g c a t t a g a c g a

120 ori g c t t g c a a a c a a a a a a a c c a c c g c t a c c a g c g g t g g t t t g t t t g c c g g a t c a a g a g c t a c c g a a c g t t t g t t t t t t t g g t g g c g a t g g t c g c c a c c a a a c a a a c g g c c t a g t t c t c g a t g

180 ori c a a c t c t t t t t c c g a a g g t a a c t g g c t t c a g c a g a g c g c a g a t a c c a a a t a c t g t t c t t c g t t g a g a a a a a g g c t t c c a t t g a c c g a a g t c g t c t c g c g t c t a t g g t t t a t g a c a a g a a g

240 ori t a g t g t a g c c g t a g t t a g g c c a c c a c t t c a a g a a c t c t g t a g c a c c g c c t a c a t a c c t c g a t c a c a t c g g c a t c a a t c c g g t g g t g a a g t t c t t g a g a c a t c g t g g c g g a t g t a t g g a g c

300 ori c t c t g c t a a t c c t g t t a c c a g t g g c t g c t g c c a g t g g c g a t a a g t c g t g t c t t a c c g g g t g a g a c g a t t a g g a c a a t g g t c a c c g a c g a c g g t c a c c g c t a t t c a g c a c a g a a t g g c c c a

360 ori t g g a c t c a a g a c g a t a g t t a c c g g a t a a g g c g c a g c g g t c g g g c t g a a c g g g g g g t t c g t a c c t g a g t t c t g c t a t c a a t g g c c t a t t c c g c g t c g c c a g c c c g a c t t g c c c c c c a a g c a

420 ori g c a c a c a g c c c a g c t t g g a g c g a a c g a c c t a c a c c g a a c t g a g a t a c c t a c a g c g t g a g c c g t g t g t c g g g t c g a a c c t c g c t t g c t g g a t g t g g c t t g a c t c t a t g g a t g t c g c a c t c g

480 ori t a t g a g a a a g c g c c a c g c t t c c c g a a g g g a g a a a g g c g g a c a g g t a t c c g g t a a g c g g c a a t a c t c t t t c g c g g t g c g a a g g g c t t c c c t c t t t c c g c c t g t c c a t a g g c c a t t c g c c g t

540 ori G G G A A A C G C C T G G T A T C T T T pBR322ori-F g g g t c g g a a c a g g a g a g c g c a c g a g g g a g c t t c c a g g g g g a a a c g c c t g g t a t c t t t a t a c c c a g c c t t g t c c t c t c g c g t g c t c c c t c g a a g g t c c c c c t t t g c g g a c c a t a g a a a t a t

600 ori g t c c t g t c g g g t t t c g c c a c c t c t g a c t t g a g c g t c g a t t t t t g t g a t g c t c g t c a g g g g c a g g a c a g c c c a a a g c g g t g g a g a c t g a a c t c g c a g c t a a a a a c a c t a c g a g c a g t c c c c

660 ori g g c g g a g c c t a t g g a a a a a c g c c a g c a a c g c g g c c t t t t t a c g g t t c c t g g c c t t t t g c t c c g c c t c g g a t a c c t t t t t g c g g t c g t t g c g c c g g a a a a a t g c c a a g g a c c g g a a a a c g a

PciI 720 g g c c t t t t g c t c a c a t g t t c t t t c c t g c g t t a t c c c c t g a t t c t g t g g a t a a c c g t a t t a c c g g a a a a c g a g t g t a c a a g a a a g g a c g c a a t a g g g g a c t a a g a c a c c t a t t g g c a t a a t

AseI 780 c c g c c a t g c a t t a g t t a t t a a t a g t a a t c a a t t a c g g g g t c a t t a g t t c a t a g c c c a t a t g g c g g t a c g t a a t c a a t a a t t a t c a t t a g t t a a t g c c c c a g t a a t c a a g t a t c g g g t a t a

840 CMV enhancer a t g g a g t t c c g c g t t a c a t a a c t t a c g g t a a a t g g c c c g c c t g g c t g a c c g c c c a a c g a c t a c c t c a a g g c g c a a t g t a t t g a a t g c c a t t t a c c g g g c g g a c c g a c t g g c g g g t t g c t g

900 CMV enhancer c c c c g c c c a t t g a c g t c a a t a a t g a c g t a t g t t c c c a t a g t a a c g c c a a t a g g g a c t t t c g g g g c g g g t a a c t g c a g t t a t t a c t g c a t a c a a g g g t a t c a t t g c g g t t a t c c c t g a a a g

960 CMV enhancer c a t t g a c g t c a a t g g g t g g a g t a t t t a c g g t a a a c t g c c c a c t t g g c a g t a c a t c a a g t g g t a a c t g c a g t t a c c c a c c t c a t a a a t g c c a t t t g a c g g g t g a a c c g t c a t g t a g t t c a c

NdeI 1020 CMV enhancer t a t c a t a t g c c a a g t a c g c c c c c t a t t g a c g t c a a t g a c g g t a a a t g g c c c g c c t g g c a t a t a g t a t a c g g t t c a t g c g g g g g a t a a c t g c a g t t a c t g c c a t t t a c c g g g c g g a c c g t a

SnaBI 1080 CMV enhancer t a t g c c c a g t a c a t g a c c t t a t g g g a c t t t c c t a c t t g g c a g t a c a t c t a c g t a t t a g t c a t a c g g g t c a t g t a c t g g a a t a c c c t g a a a g g a t g a a c c g t c a t g t a g a t g c a t a a t c a g

1140 CMV enhancer CMV promoter a t c g c t a t t a c c a t g g t g a t g c g g t t t t g g c a g t a c a t c a a t g g g c g t g g a t a g c g g t t t t a g c g a t a a t g g t a c c a c t a c g c c a a a a c c g t c a t g t a g t t a c c c g c a c c t a t c g c c a a a

1200 CMV promoter g a c t c a c g g g g a t t t c c a a g t c t c c a c c c c a t t g a c g t c a a t g g g a g t t t g t t t t g g c a c c t g a g t g c c c c t a a a g g t t c a g a g g t g g g g t a a c t g c a g t t a c c c t c a a a c a a a a c c g t g

1260 CMV promoter C G C A A A T G G G C CMV-F c a a a a t c a a c g g g a c t t t c c a a a a t g t c g t a a c a a c t c c g c c c c a t t g a c g c a a a t g g g c g t t t t a g t t g c c c t g a a a g g t t t t a c a g c a t t g t t g a g g c g g g g t a a c t g c g t t t a c c c g

1320 CMV promoter G G T A G G C G T G CMV-F g g t a g g c g t g t a c g g t g g g a g g t c t a t a t a a g c a g a g c t g g t t t a g t g a a c c g t c a g a t c c c a t c c g c a c a t g c c a c c c t c c a g a t a t a t t c g t c t c g a c c a a a t c a c t t g g c a g t c t a g

BmtINheI 1380 Kozak sequence MALDGIRMPDGCYAD c g c t a g c g g c c a c c a t g g c t c t g g a c g g g a t a a g g a t g c c a g a t g g c t g c t a c g c g g a c g g c g a t c g c c g g t g g t a c c g a g a c c t g c c c t a t t c c t a c g g t c t a c c g a c g a t g c g c c t g c

1440 GTWELSVHVTDLNRDVTLRV g g a c g t g g g a a c t g a g t g t c c a t g t g a c g g a c c t g a a c c g c g a t g t c a c c c t g a g a g t g a c c t g c a c c c t t g a c t c a c a g g t a c a c t g c c t g g a c t t g g c g c t a c a g t g g g a c t c t c a c t

1500 TGEVHIGGVMLKLVEKLDVK c c g g c g a g g t g c a c a t t g g a g g c g t g a t g c t t a a g c t g g t g g a g a a a c t c g a t g t a a a a a g g c c g c t c c a c g t g t a a c c t c c g c a c t a c g a a t t c g a c c a c c t c t t t g a g c t a c a t t t t t

1560 KDWSDHALWWEKKRTWLLKT a a g a t t g g t c t g a c c a t g c t c t c t g g t g g g a a a a g a a g a g a a c t t g g c t t c t g a a g a c a c t t c t a a c c a g a c t g g t a c g a g a g a c c a c c c t t t t c t t c t c t t g a a c c g a a g a c t t c t g t g

BbvCIBlpI 1620 HWTLDKYGIQADAKLQFTPQ a t t g g a c c t t a g a t a a g t a t g g t a t t c a g g c a g a t g c t a a g c t t c a g t t c a c c c c t c a g c t a a c c t g g a a t c t a t t c a t a c c a t a a g t c c g t c t a c g a t t c g a a g t c a a g t g g g g a g t c g

1680 HKLLRLQLPNMKYVKVKVNF a c a a a c t g c t c c g c c t g c a g c t t c c c a a c a t g a a g t a t g t g a a g g t g a a a g t g a a t t t c t t g t t t g a c g a g g c g g a c g t c g a a g g g t t g t a c t t c a t a c a c t t c c a c t t t c a c t t a a a g a

1740 SDRVFKAVSDICKTFNIRHP c t g a t a g a g t c t t c a a a g c t g t t t c t g a c a t c t g t a a g a c t t t t a a t a t c a g a c a c c c c g g a c t a t c t c a g a a g t t t c g a c a a a g a c t g t a g a c a t t c t g a a a a t t a t a g t c t g t g g g g c

1800 EELSLLKKPRDPTKKKKKKL a a g a a c t t t c t c t c t t a a a g a a a c c c a g a g a t c c a a c a a a g a a a a a a a a g a a g a a g c t a g t t c t t g a a a g a g a g a a t t t c t t t g g g t c t c t a g g t t g t t t c t t t t t t t t c t t c t t c g a t c

EcoNI 1860 DDQSEDEALELEGPLITPGS a t g a c c a g t c t g a a g a t g a g g c a c t t g a a t t a g a g g g g c c t c t t a t c a c t c c t g g a t c a g t a c t g g t c a g a c t t c t a c t c c g t g a a c t t a a t c t c c c c g g a g a a t a g t g a g g a c c t a g t c

1920 GSIYSAPGLYSKTMTPTYDA g a a g t a t a t a t t c a g c g c c a g g a c t g t a t a g t a a a a c a a t g a c c c c c a c t t a t g a t g c t c c t t c a t a t a t a a g t c g c g g t c c t g a c a t a t c a t t t t g t t a c t g g g g g t g a a t a c t a c g a g

1980 HDGSPLSPTSAWFGDSALSE a t g a t g g a a g c c c c t t g t c a c c a a c t t c t g c t t g g t t t g g t g a c a g t g c t t t g t c a g a a g t a c t a c c t t c g g g g a a c a g t g g t t g a a g a c g a a c c a a a c c a c t g t c a c g a a a c a g t c t t c

AccIBstZ17I 2040 GNPGILAVSQPITSPEILAK g c a a t c c t g g t a t a c t t g c t g t c a g t c a a c c a a t c a c g t c a c c a g a a a t c t t g g c a a a a a c g t t a g g a c c a t a t g a a c g a c a g t c a g t t g g t t a g t g c a g t g g t c t t t a g a a c c g t t t t t

2100 MFKPQALLDKAKINQGWLDS t g t t c a a g c c t c a a g c t c t t c t t g a t a a a g c a a a a a t c a a c c a a g g a t g g c t t g a t t c c t a c a a g t t c g g a g t t c g a g a a g a a c t a t t t c g t t t t t a g t t g g t t c c t a c c g a a c t a a g g a

BglII 2160 SRSLMEQDVKENEALLLRFK c a a g a t c t c t c a t g g a a c a a g a t g t g a a g g a a a a t g a g g c c t t g c t g c t c c g a t t c a a g t g t t c t a g a g a g t a c c t t g t t c t a c a c t t c c t t t t a c t c c g g a a c g a c g a g g c t a a g t t c a

2220 YYSFFDLNPKYDAIRINQLY a t t a c a g c t t t t t t g a t t t g a a t c c a a a g t a t g a t g c a a t c a g a a t c a a t c a g c t t t a t g t a a t g t c g a a a a a a c t a a a c t t a g g t t t c a t a c t a c g t t a g t c t t a g t t a g t c g a a a t a c

2280 EQAKWAILLEEIECTEEEMM a g c a g g c c a a a t g g g c c a t t c t c c t g g a a g a g a t t g a a t g c a c a g a a g a a g a a a t g a t g a t c g t c c g g t t t a c c c g g t a a g a g g a c c t t c t c t a a c t t a c g t g t c t t c t t c t t t a c t a c t

2340 MFAALQYHINKLSIMTSENH t g t t t g c a g c c c t g c a g t a t c a t a t c a a t a a g c t g t c a a t c a t g a c a t c a g a g a a t c a t t a c a a a c g t c g g g a c g t c a t a g t a t a g t t a t t c g a c a g t t a g t a c t g t a g t c t c t t a g t a a

2400 LNNSDKEVDEVDAALSDLEI t g a a c a a c a g t g a c a a a g a a g t t g a t g a a g t t g a t g c t g c c c t t t c a g a c c t g g a g a t t a a c t t g t t g t c a c t g t t t c t t c a a c t a c t t c a a c t a c g a c g g g a a a g t c t g g a c c t c t a a t

2460 TLEGGKTSTILGDITSIPEL c t c t g g a a g g g g g t a a a a c g t c a a c a a t t t t g g g t g a c a t t a c t t c c a t t c c t g a a c t t g g a g a c c t t c c c c c a t t t t g c a g t t g t t a a a a c c c a c t g t a a t g a a g g t a a g g a c t t g a a c

2520 ADYIKVFKPKKLTLKGYKQY c t g a c t a c a t t a a a g t t t t c a a g c c a a a a a a g c t g a c t c t g a a a g g t t a c a a a c a a t a t t g a c t g a t g t a a t t t c a a a a g t t c g g t t t t t t c g a c t g a g a c t t t c c a a t g t t t g t t a t a a

2580 WCTFKDTSISCYKSKEESSG g g t g c a c c t t c a a a g a c a c a t c c a t t t c t t g t t a t a a g a g c a a a g a a g a a t c c a g t g g c a c c a c g t g g a a g t t t c t g t g t a g g t a a a g a a c a a t a t t c t c g t t t c t t c t t a g g t c a c c g t

2640 TPAHQMNLRGCEVTPDVNIS c a c c a g c t c a t c a g a t g a a c c t c a g g g g a t g t g a a g t t a c c c c a g a t g t a a a c a t t t c a g g t g g t c g a g t a g t c t a c t t g g a g t c c c c t a c a c t t c a a t g g g g t c t a c a t t t g t a a a g t c

2700 GQKFNIKLLIPVAEGMNEIW g c c a a a a a t t t a a c a t t a a a c t c c t g a t t c c a g t t g c a g a a g g c a t g a a t g a a a t c t g g c c g g t t t t t a a a t t g t a a t t t g a g g a c t a a g g t c a a c g t c t t c c g t a c t t a c t t t a g a c c g

2760 LRCDNEKQYAHWMAACRLAS t t c g t t g t g a c a a t g a a a a a c a g t a t g c a c a c t g g a t g g c a g c c t g c a g a t t a g c c t c c a a a g c a a c a c t g t t a c t t t t t g t c a t a c g t g t g a c c t a c c g t c g g a c g t c t a a t c g g a g g t

2820 KGKTMADSSYNLEVQNILSF a a g g c a a g a c c a t g g c g g a c a g t t c t t a c a a c t t a g a a g t t c a g a a t a t t c t t t c c t t t c t t c c g t t c t g g t a c c g c c t g t c a a g a a t g t t g a a t c t t c a a g t c t t a t a a g a a a g g a a a g

2880 LKMQHLNPDPQLIPEQITTD t g a a g a t g c a g c a t t t a a a c c c a g a t c c t c a g t t a a t a c c a g a g c a g a t c a c g a c t g a t a a c t t c t a c g t c g t a a a t t t g g g t c t a g g a g t c a a t t a t g g t c t c g t c t a g t g c t g a c t a t

2940 ITPECLVSPRYLKKYKNKQI t a a c t c c t g a a t g t t t g g t g t c t c c c c g c t a t c t a a a a a a g t a t a a g a a c a a g c a g a t a a a t t g a g g a c t t a c a a a c c a c a g a g g g g c g a t a g a t t t t t t c a t a t t c t t g t t c g t c t a t t

3000 TARILEAHQNVAQMSLIEAK c a g c g a g a a t c t t g g a g g c c c a t c a g a a t g t a g c t c a g a t g a g t c t a a t t g a a g c c a a g a g t c g c t c t t a g a a c c t c c g g g t a g t c t t a c a t c g a g t c t a c t c a g a t t a a c t t c g g t t c t

3060 MRFIQAWQSLPEFGITHFIA t g a g a t t t a t t c a a g c t t g g c a g t c a c t a c c t g a a t t t g g c a t c a c t c a c t t c a t t g c a a a c t c t a a a t a a g t t c g a a c c g t c a g t g a t g g a c t t a a a c c g t a g t g a g t g a a g t a a c g t t

3120 RFQGGKKEELIGIAYNRLIR g g t t c c a a g g g g g c a a a a a a g a a g a a c t t a t t g g a a t t g c a t a c a a c a g a c t g a t t c g g a c c a a g g t t c c c c c g t t t t t t c t t c t t g a a t a a c c t t a a c g t a t g t t g t c t g a c t a a g c c t

3180 MDASTGDAIKTWRFSNMKQW t g g a t g c c a g c a c t g g a g a t g c a a t t a a a a c a t g g c g t t t c a g c a a c a t g a a a c a g t g g a a c c t a c g g t c g t g a c c t c t a c g t t a a t t t t g t a c c g c a a a g t c g t t g t a c t t t g t c a c c t

BstEII 3240 NVNWEIKMVTVEFADEVRLS a t g t c a a c t g g g a a a t c a a a a t g g t c a c c g t a g a g t t t g c a g a t g a a g t a c g a t t g t c c t t a c a g t t g a c c c t t t a g t t t t a c c a g t g g c a t c t c a a a c g t c t a c t t c a t g c t a a c a g g a

EcoRI 3300 FICTEVDCKVVHEFIGGYIF t c a t t t g t a c t g a a g t a g a t t g c a a a g t g g t t c a t g a a t t c a t t g g t g g c t a c a t a t t t c a g t a a a c a t g a c t t c a t c t a a c g t t t c a c c a a g t a c t t a a g t a a c c a c c g a t g t a t a a a g

PmlI 3360 LSTRAKDQNESLDEEMFYKL t c t c a a c a c g t g c a a a a g a c c a a a a c g a g a g t t t a g a t g a a g a g a t g t t c t a c a a a c t t a a g a g t t g t g c a c g t t t t c t g g t t t t g c t c t c a a a t c t a c t t c t c t a c a a g a t g t t t g a a t

BamHIPflMI 3420 1 M 1a V 5 SKGEEL EGFP 1 M 1a V 5 SKGEEL EGFP TSGWVGNSGDPGMVSKGEEL c c a g t g g t t g g g t g g g t a a c t c a g g g g a t c c a g g a a t g g t g a g c a a g g g c g a g g a g c t g t g g t c a c c a a c c c a c c c a t t g a g t c c c c t a g g t c c t t a c c a c t c g t t c c c g c t c c t c g a c a

3480 10 15 20 25 FTGVVPILVELDGDVNGHKF EGFP 10 15 20 25 FTGVVPILVELDGDVNGHKF EGFP G A C C A G C T C G A C C T G C C G C T G C EGFP-N FTGVVPILVELDGDVNGHKF t c a c c g g g g t g g t g c c c a t c c t g g t c g a g c t g g a c g g c g a c g t a a a c g g c c a c a a g t t c a a g t g g c c c c a c c a c g g g t a g g a c c a g c t c g a c c t g c c g c t g c a t t t g c c g g t g t t c a a g t

3540 30 35 40 45 SVSGEGEGDATYGKLTLKFI EGFP 30 35 40 45 SVSGEGEGDATYGKLTLKFI EGFP SVSGEGEGDATYGKLTLKFI g c g t g t c c g g c g a g g g c g a g g g c g a t g c c a c c t a c g g c a a g c t g a c c c t g a a g t t c a t c t c g c a c a g g c c g c t c c c g c t c c c g c t a c g g t g g a t g c c g t t c g a c t g g g a c t t c a a g t a g a

3600 50 55 60 65 CTTGKLPVPWPTLVTTLTYG EGFP 50 55 60 65 CTTGKLPVPWPTLVTTLTYG EGFP CTTGKLPVPWPTLVTTLTYG g c a c c a c c g g c a a g c t g c c c g t g c c c t g g c c c a c c c t c g t g a c c a c c c t g a c c t a c g g c g c g t g g t g g c c g t t c g a c g g g c a c g g g a c c g g g t g g g a g c a c t g g t g g g a c t g g a t g c c g c

3660 70 75 80 85 VQCFSRYPDHMKQHDFFKSA EGFP 70 75 80 85 VQCFSRYPDHMKQHDFFKSA EGFP VQCFSRYPDHMKQHDFFKSA t g c a g t g c t t c a g c c g c t a c c c c g a c c a c a t g a a g c a g c a c g a c t t c t t c a a g t c c g c c a a c g t c a c g a a g t c g g c g a t g g g g c t g g t g t a c t t c g t c g t g c t g a a g a a g t t c a g g c g g t

3720 90 95 100 105 MPEGYVQERTIFFKDDGNYK EGFP 90 95 100 105 MPEGYVQERTIFFKDDGNYK EGFP C T G C T G C C G T T G A T G T T C T EXFP-R MPEGYVQERTIFFKDDGNYK t g c c c g a a g g c t a c g t c c a g g a g c g c a c c a t c t t c t t c a a g g a c g a c g g c a a c t a c a a g a a c g g g c t t c c g a t g c a g g t c c t c g c g t g g t a g a a g a a g t t c c t g c t g c c g t t g a t g t t c t

3780 110 115 120 125 TRAEVKFEGDTLVNRIELKG EGFP 110 115 120 125 TRAEVKFEGDTLVNRIELKG EGFP G EXFP-R TRAEVKFEGDTLVNRIELKG c c c g c g c c g a g g t g a a g t t c g a g g g c g a c a c c c t g g t g a a c c g c a t c g a g c t g a a g g g c a g g g c g c g g c t c c a c t t c a a g c t c c c g c t g t g g g a c c a c t t g g c g t a g c t c g a c t t c c c g t

3840 130 135 140 145 IDFKEDGNILGHKLEYNYNS EGFP 130 135 140 145 IDFKEDGNILGHKLEYNYNS EGFP IDFKEDGNILGHKLEYNYNS t c g a c t t c a a g g a g g a c g g c a a c a t c c t g g g g c a c a a g c t g g a g t a c a a c t a c a a c a g c c a g c t g a a g t t c c t c c t g c c g t t g t a g g a c c c c g t g t t c g a c c t c a t g t t g a t g t t g t c g g

3900 150 155 160 165 HNVYIMADKQKNGIKVNFKI EGFP 150 155 160 165 HNVYIMADKQKNGIKVNFKI EGFP HNVYIMADKQKNGIKVNFKI a c a a c g t c t a t a t c a t g g c c g a c a a g c a g a a g a a c g g c a t c a a g g t g a a c t t c a a g a t c c t g t t g c a g a t a t a g t a c c g g c t g t t c g t c t t c t t g c c g t a g t t c c a c t t g a a g t t c t a g g

3960 170 175 180 185 RHNIEDGSVQLADHYQQNTP EGFP 170 175 180 185 RHNIEDGSVQLADHYQQNTP EGFP RHNIEDGSVQLADHYQQNTP g c c a c a a c a t c g a g g a c g g c a g c g t g c a g c t c g c c g a c c a c t a c c a g c a g a a c a c c c c c a c g g t g t t g t a g c t c c t g c c g t c g c a c g t c g a g c g g c t g g t g a t g g t c g t c t t g t g g g g g t

4020 190 195 200 205 IGDGPVLLPDNHYLSTQSAL EGFP 190 195 200 205 IGDGPVLLPDNHYLSTQSAL EGFP IGDGPVLLPDNHYLSTQSAL t c g g c g a c g g c c c c g t g c t g c t g c c c g a c a a c c a c t a c c t g a g c a c c c a g t c c g c c c t g a a g c c g c t g c c g g g g c a c g a c g a c g g g c t g t t g g t g a t g g a c t c g t g g g t c a g g c g g g a c t

4080 210 215 220 225 SKDPNEKRDHMVLLEFVTAA EGFP 210 215 220 225 SKDPNEKRDHMVLLEFVTAA EGFP C A T G G T C C T G C T G G A G T T C G T G EGFP-C SKDPNEKRDHMVLLEFVTAA g c a a a g a c c c c a a c g a g a a g c g c g a t c a c a t g g t c c t g c t g g a g t t c g t g a c c g c c g c c g c g t t t c t g g g g t t g c t c t t c g c g c t a g t g t a c c a g g a c g a c c t c a a g c a c t g g c g g c g g c

XbaI*NotIBsrGI 4140 230 235 GITLGMDELYK* EGFP 230 235 GITLGMDELYK * EGFP GITLGMDELYK* g g a t c a c t c t c g g c a t g g a c g a g c t g t a c a a g t a a a g c g g c c g c g a c t c t a g a t c a t a a t c c t a g t g a g a g c c g t a c c t g c t c g a c a t g t t c a t t t c g c c g g c g c t g a g a t c t a g t a t t a

4200 c a g c c a t a c c a c a t t t g t a g a g g t t t t a c t t g c t t t a a a a a a c c t c c c a c a c c t c c c c c t g t c g g t a t g g t g t a a a c a t c t c c a a a a t g a a c g a a a t t t t t t g g a g g g t g t g g a g g g g g a

HpaIMfeI 4260 SV40 poly(A) signal g a a c c t g a a a c a t a a a a t g a a t g c a a t t g t t g t t g t t a a c t t g t t t a t t g c a g c t t a t a a c t t g g a c t t t g t a t t t t a c t t a c g t t a a c a a c a a c a a t t g a a c a a a t a a c g t c g a a t a t t

4320 SV40 poly(A) signal C G T T A T C G T A G T G T T T A A A G SV40pA-R t g g t t a c a a a t a a a g c a a t a g c a t c a c a a a t t t c a c a a a t a a a g c a t t t t t t t c a c t g c a a c c a a t g t t t a t t t c g t t a t c g t a g t g t t t a a a g t g t t t a t t t c g t a a a a a a a g t g a c g t

4380 SV40 poly(A) signal f1 ori G T G G T T T G T C C A A A C T C A T C EBV-rev t t c t a g t t g t g g t t t g t c c a a a c t c a t c a a t g t a t c t t a a g g c g t a a a t t g t a a g c g t t a a a g a t c a a c a c c a a a c a g g t t t g a g t a g t t a c a t a g a a t t c c g c a t t t a a c a t t c g c a a t

4440 f1 ori a t a t t t t g t t a a a a t t c g c g t t a a a t t t t t g t t a a a t c a g c t c a t t t t t t a a c c a a t a g g t a t a a a a c a a t t t t a a g c g c a a t t t a a a a a c a a t t t a g t c g a g t a a a a a a t t g g t t a t c c

4500 f1 ori c c g a a a t c g g c a a a a t c c c t t a t a a a t c a a a a g a a t a g a c c g a g a t a g g g t t g a g t g t t g g g c t t t a g c c g t t t t a g g g a a t a t t t a g t t t t c t t a t c t g g c t c t a t c c c a a c t c a c a a c

4560 f1 ori G G T C A A A C C T T G T T C T C A G G T G F1ori-F t t c c a g t t t g g a a c a a g a g t c c a c t a t t a a a g a a c g t g g a c t c c a a c g t c a a a g g g c g a a a a g g t c a a a c c t t g t t c t c a g g t g a t a a t t t c t t g c a c c t g a g g t t g c a g t t t c c c g c t t

DraIII 4620 f1 ori a a a c c g t c t a t c a g g g c g a t g g c c c a c t a c g t g a a c c a t c a c c c t a a t c a a g t t t t t t g g t t t g g c a g a t a g t c c c g c t a c c g g g t g a t g c a c t t g g t a g t g g g a t t a g t t c a a a a a a c c

4680 f1 ori g g t c g a g g t g c c g t a a a g c a c t a a a t c g g a a c c c t a a a g g g a g c c c c c g a t t t a g a g c t t c c a g c t c c a c g g c a t t t c g t g a t t t a g c c t t g g g a t t t c c c t c g g g g g c t a a a t c t c g a a

4740 f1 ori A G G G A A G A A A G C G A A A G G A G F1ori-R g a c g g g g a a a g c c g g c g a a c g t g g c g a g a a a g g a a g g g a a g a a a g c g a a a g g a g c g g g c g c t g c c c c t t t c g g c c g c t t g c a c c g c t c t t t c c t t c c c t t c t t t c g c t t t c c t c g c c c g c

4800 f1 ori c t a g g g c g c t g g c a a g t g t a g c g g t c a c g c t g c g c g t a a c c a c c a c a c c c g c c g c g c t t a g a t c c c g c g a c c g t t c a c a t c g c c a g t g c g a c g c g c a t t g g t g g t g t g g g c g g c g c g a a t

4860 f1 ori AmpR promoter a t g c g c c g c t a c a g g g c g c g t c a g g t g g c a c t t t t c g g g g a a a t g t g c g c g g a a c c c c t a t a c g c g g c g a t g t c c c g c g c a g t c c a c c g t g a a a a g c c c c t t t a c a c g c g c c t t g g g g a t

4920 AmpR promoter t t t g t t t a t t t t t c t a a a t a c a t t c a a a t a t g t a t c c g c t c a t g a g a c a a t a a c c c t g a t a a a c a a a t a a a a a g a t t t a t g t a a g t t t a t a c a t a g g c g a g t a c t c t g t t a t t g g g a c t a

4980 AmpR promoter SV40 promoter SV40 enhancer C C T T pBABE 3' a a a t g c t t c a a t a a t a t t g a a a a a g g a a g a g t c c t g a g g c g g a a a g a a c c a g c t g t g g a a t t t a c g a a g t t a t t a t a a c t t t t t c c t t c t c a g g a c t c c g c c t t t c t t g g t c g a c a c c t t

5040 SV40 promoter SV40 enhancer A C A C A C A G T C A A T C C C A pBABE 3' t g t g t g t c a g t t a g g g t g t g g a a a g t c c c c a g g c t c c c c a g c a g g c a g a a g t a t g c a a a g a c a c a c a g t c a a t c c c a c a c c t t t c a g g g g t c c g a g g g g t c g t c c g t c t t c a t a c g t t t c

SexAI*CsiI 5100 SV40 promoter SV40 enhancer c a t g c a t c t c a a t t a g t c a g c a a c c a g g t g t g g a a a g t c c c c a g g c t c c c c a g c a g g c a g g t a c g t a g a g t t a a t c a g t c g t t g g t c c a c a c c t t t c a g g g g t c c g a g g g g t c g t c c g t c

5160 SV40 promoter SV40 enhancer a a g t a t g c a a a g c a t g c a t c t c a a t t a g t c a g c a a c c a t a g t c c c g c c c c t a a c t c c g c c t t c a t a c g t t t c g t a c g t a g a g t t a a t c a g t c g t t g g t a t c a g g g c g g g g a t t g a g g c g g

5220 SV40 promoter SV40 ori c a t c c c g c c c c t a a c t c c g c c c a g t t c c g c c c a t t c t c c g c c c c a t g g c t g a c t a a t t t t g t a g g g c g g g g a t t g a g g c g g g t c a a g g c g g g t a a g a g g c g g g g t a c c g a c t g a t t a a a a

5280 SV40 promoter SV40 ori t t t t a t t t a t g c a g a g g c c g a g g c c g c c t c g g c c t c t g a g c t a t t c c a g a a g t a g t g a g g a a a a t a a a t a c g t c t c c g g c t c c g g c g g a g c c g g a g a c t c g a t a a g g t c t t c a t c a c t c c

BspDI*ClaI* 5340 SV40 promoter SV40 ori a g g c t t t t t t g g a g g c c t a g g c t t t t g c a a a g a t c g a t c a a g a g a c a g g a t g a g g a t c g t t c c g a a a a a a c c t c c g g a t c c g a a a a c g t t t c t a g c t a g t t c t c t g t c c t a c t c c t a g c a

5400 1 5 10 15 MIEQDGLHAGSPAAWVER NeoR/KanR 1 5 10 15 MIEQDGLHAGSPAAWVER NeoR/KanR G Neo-R MIEQDGLHAGSPAAWVER t t c g c a t g a t t g a a c a a g a t g g a t t g c a c g c a g g t t c t c c g g c c g c t t g g g t g g a g a g g c a a g c g t a c t a a c t t g t t c t a c c t a a c g t g c g t c c a a g a g g c c g g c g a a c c c a c c t c t c c g

5460 20 25 30 35 LFGYDWAQQTIGCSDAAVFR NeoR/KanR 20 25 30 35 LFGYDWAQQTIGCSDAAVFR NeoR/KanR A T A A G C C G A T A C T G A C C C G Neo-R LFGYDWAQQTIGCSDAAVFR t a t t c g g c t a t g a c t g g g c a c a a c a g a c a a t c g g c t g c t c t g a t g c c g c c g t g t t c c g g c a t a a g c c g a t a c t g a c c c g t g t t g t c t g t t a g c c g a c g a g a c t a c g g c g g c a c a a g g c c g

KasINarISfoIPluTI 5520 40 45 50 55 LSAQGRPVLFVKTDLSGALN NeoR/KanR 40 45 50 55 LSAQGRPVLFVKTDLSGALN NeoR/KanR LSAQGRPVLFVKTDLSGALN t g t c a g c g c a g g g g c g c c c g g t t c t t t t t g t c a a g a c c g a c c t g t c c g g t g c c c t g a a t g a c a g t c g c g t c c c c g c g g g c c a a g a a a a a c a g t t c t g g c t g g a c a g g c c a c g g g a c t t a c

FspIMscI 5580 60 65 70 75 ELQDEAARLSWLATTGVPCA NeoR/KanR 60 65 70 75 ELQDEAARLSWLATTGVPCA NeoR/KanR ELQDEAARLSWLATTGVPCA a a c t g c a a g a c g a g g c a g c g c g g c t a t c g t g g c t g g c c a c g a c g g g c g t t c c t t g c g c a g t t g a c g t t c t g c t c c g t c g c g c c g a t a g c a c c g a c c g g t g c t g c c c g c a a g g a a c g c g t c

Tth111IPflFI 5640 80 85 90 95 AVLDVVTEAGRDWLLLGEVP NeoR/KanR 80 85 90 95 AVLDVVTEAGRDWLLLGEVP NeoR/KanR AVLDVVTEAGRDWLLLGEVP c t g t g c t c g a c g t t g t c a c t g a a g c g g g a a g g g a c t g g c t g c t a t t g g g c g a a g t g c c g g g a c a c g a g c t g c a a c a g t g a c t t c g c c c t t c c c t g a c c g a c g a t a a c c c g c t t c a c g g c c

5700 100 105 110 115 GQDLLSSHLAPAEKVSIMAD NeoR/KanR 100 105 110 115 GQDLLSSHLAPAEKVSIMAD NeoR/KanR GQDLLSSHLAPAEKVSIMAD *RVKSRGLFYGDHSI g g c a g g a t c t c c t g t c a t c t c a c c t t g c t c c t g c c g a g a a a g t a t c c a t c a t g g c t g a t g c c g t c c t a g a g g a c a g t a g a g t g g a a c g a g g a c g g c t c t t t c a t a g g t a g t a c c g a c t a c

5760 120 125 130 135 AMRRLHTLDPATCPFDHQAK NeoR/KanR 120 125 130 135 AMRRLHTLDPATCPFDHQAK NeoR/KanR AMRRLHTLDPATCPFDHQAK CHPPQMRKIRSGAWEVVLRF c a a t g c g g c g g c t g c a t a c g c t t g a t c c g g c t a c c t g c c c a t t c g a c c a c c a a g c g a a a c g t t a c g c c g c c g a c g t a t g c g a a c t a g g c c g a t g g a c g g g t a a g c t g g t g g t t c g c t t t g

5820 140 145 150 155 HRIERARTRMEAGLVDQDDL NeoR/KanR 140 145 150 155 HRIERARTRMEAGLVDQDDL NeoR/KanR HRIERARTRMEAGLVDQDDL MADLSCTSPHFGTKDILIIQ a t c g c a t c g a g c g a g c a c g t a c t c g g a t g g a a g c c g g t c t t g t c g a t c a g g a t g a t c t g g t a g c g t a g c t c g c t c g t g c a t g a g c c t a c c t t c g g c c a g a a c a g c t a g t c c t a c t a g a c c

5880 160 165 170 175 DEEHQGLAPAELFARLKASM NeoR/KanR 160 165 170 175 DEEHQGLAPAELFARLKASM NeoR/KanR DEEHQGLAPAELFARLKASM VFLMLPERWGFQEGPELRAH a c g a a g a g c a t c a g g g g c t c g c g c c a g c c g a a c t g t t c g c c a g g c t c a a g g c g a g c a t g c t g c t t c t c g t a g t c c c c g a g c g c g g t c g g c t t g a c a a g c g g t c c g a g t t c c g c t c g t a c g

5940 180 185 190 195 PDGEDLVVTHGDACLPNIMV NeoR/KanR 180 185 190 195 PDGEDLVVTHGDACLPNIMV NeoR/KanR PDGEDLVVTHGDACLPNIMV GVALIEDHGMAIGAQRIDHH c c g a c g g c g a g g a t c t c g t c g t g a c c c a t g g c g a t g c c t g c t t g c c g a a t a t c a t g g t g g g g c t g c c g c t c c t a g a g c a g c a c t g g g t a c c g c t a c g g a c g a a c g g c t t a t a g t a c c a c c

RsrII 6000 200 205 210 215 ENGRFSGFIDCGRLGVADRY NeoR/KanR 200 205 210 215 ENGRFSGFIDCGRLGVADRY NeoR/KanR ENGRFSGFIDCGRLGVADRY FIAAKRSEDVTAPQTHRVAI a a a a t g g c c g c t t t t c t g g a t t c a t c g a c t g t g g c c g g c t g g g t g t g g c g g a c c g c t a t c t t t t a c c g g c g a a a a g a c c t a a g t a g c t g a c a c c g g c c g a c c c a c a c c g c c t g g c g a t a g

6060 220 225 230 235 QDIALATRDIAEELGGEWAD NeoR/KanR 220 225 230 235 QDIALATRDIAEELGGEWAD NeoR/KanR C G T T G G C T A C C C G T G A T A T T Neo-F QDIALATRDIAEELGGEWAD LVYRQSGTINSFLKAAFPSV a g g a c a t a g c g t t g g c t a c c c g t g a t a t t g c t g a a g a g c t t g g c g g c g a a t g g g c t g a c c t c c t g t a t c g c a a c c g a t g g g c a c t a t a a c g a c t t c t c g a a c c g c c g c t t a c c c g a c t g g

6120 240 245 250 255 RFLVLYGIAAPDSQRIAFYR NeoR/KanR 240 245 250 255 RFLVLYGIAAPDSQRIAFYR NeoR/KanR RFLVLYGIAAPDSQRIAFYR AEEHKVTDGSGIRLADGEIA g c t t c c t c g t g c t t t a c g g t a t c g c c g c t c c c g a t t c g c a g c g c a t c g c c t t c t a t c g c c c g a a g g a g c a c g a a a t g c c a t a g c g g c g a g g g c t a a g c g t c g c g t a g c g g a a g a t a g c g g

BstBI 6180 260 265 LLDEFF* NeoR/KanR 260 265 LLDEFF* NeoR/KanR MTDQATP LLDEFF* KKVLEESRSEPTRFSRGLSA t t c t t g a c g a g t t c t t c t g a g c g g g a c t c t g g g g t t c g a a a t g a c c g a c c a a g c g a c g c c a a g a a c t g c t c a a g a a g a c t c g c c c t g a g a c c c c a a g c t t t a c t g g c t g g t t c g c t g c g g

6240 NLPSRDFDSTAAFYERLGFG WGAM c a a c c t g c c a t c a c g a g a t t t c g a t t c c a c c g c c g c c t t c t a t g a a a g g t t g g g c t t c g g g t t g g a c g g t a g t g c t c t a a a g c t a a g g t g g c g g c g g a a g a t a c t t t c c a a c c c g a a g c c

6300 IVFRDAGWMILQRGDLMLEF a a t c g t t t t c c g g g a c g c c g g c t g g a t g a t c c t c c a g c g c g g g g a t c t c a t g c t g g a g t t t t a g c a a a a g g c c c t g c g g c c g a c c t a c t a g g a g g t c g c g c c c c t a g a g t a c g a c c t c a a

6360 A C T T T G T G C C T T C C T C T G T T TK-pA-R FAHPRGRLTETRKETIPEGT c t t c g c c c a c c c t a g g g g g a g g c t a a c t g a a a c a c g g a a g g a g a c a a t a c c g g a a g g a a c g a a g c g g g t g g g a t c c c c c t c c g a t t g a c t t t g t g c c t t c c t c t g t t a t g g c c t t c c t t g

6420 HSV TK poly(A) signal RAMTAIKRQNKTHGVGSFVH c c g c g c t a t g a c g g c a a t a a a a a g a c a g a a t a a a a c g c a c g g t g t t g g g t c g t t t g t t c a g g c g c g a t a c t g c c g t t a t t t t t c t g t c t t a t t t t g c g t g c c a c a a c c c a g c a a a c a a g t

BsaI 6480 KRGVRSQGWHSVDTPPRPHW t a a a c g c g g g g t t c g g t c c c a g g g c t g g c a c t c t g t c g a t a c c c c a c c g a g a c c c c a t t g a t t t g c g c c c c a a g c c a g g g t c c c g a c c g t g a g a c a g c t a t g g g g t g g c t c t g g g g t a a c

6540 GQYARVSSFSPPHPPSSGEG g g g c c a a t a c g c c c g c g t t t c t t c c t t t t c c c c a c c c c a c c c c c c a a g t t c g g g t g a a g g c c c g g t t a t g c g g g c g c a a a g a a g g a a a a g g g g t g g g g t g g g g g g t t c a a g c c c a c t t c c

6600 PGLAANVGAAGPAIASGYSY c c c a g g g c t c g c a g c c a a c g t c g g g g c g g c a g g c c c t g c c a t a g c c t c a g g t t a c t c a t a g g g t c c c g a g c g t c g g t t g c a g c c c c g c c g t c c g g g a c g g t a t c g g a g t c c a a t g a g t a t

6660 IL* t a t a c t t t a g a t t g a t t t a a a a c t t c a t t t t t a a t t t a a a a g g a t c t a g g t g a a g a t c c t a t a t g a a a t c t a a c t a a a t t t t g a a g t a a a a a t t a a a t t t t c c t a g a t c c a c t t c t a g g a

3ʹ 5ʹ 6719 t t t t g a t a a t c t c a t g a c c a a a a t c c c t t a a c g t g a g t t t t c g t t c c a c t g a g c g t c a g a a a a c t a t t a g a g t a c t g g t t t t a g g g a a t t g c a c t c a a a a g c a a g g t g a c t c g c a g t c

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.