Addgene LRG-2.1T-neo-sgROSA Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: LRG-2.1T-neo-sgROSA Sequencing Result

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T3 (8264 .. 8284) M13 rev M13 Reverse (8229 .. 8245) M13/pUC Reverse (8210 .. 8232) lac promoter L4440 (7997 .. 8014) AflIII - PciI (7899) pBR322ori-F (7744 .. 7763) AhdI (7011) ScaI (6530) Amp-R (6442 .. 6461) F1ori-F (5935 .. 5956) DraIII (5871) F1ori-R (5725 .. 5744) M13/pUC Forward (5489 .. 5511) M13 Forward (5480 .. 5497) M13 fwd T7 (5451 .. 5470) T7 promoter AvrII (5430) StuI (5429) SfiI (5383) EBV-rev (5226 .. 5245) SV40pA-R (5172 .. 5191) SnaBI (5061) SacII (4667) AatII (4617) ZraI (4615) PflMI (4405) WPRE-R (4217 .. 4237) T3 promoter 5' LTR (truncated) HIV-1 Ψ NruI * (611) NotI (922) BbvCI (1198) BstAPI (1227) KflI - PpuMI (1708) hU6-F (1717 .. 1737) LKO.1 5' (1888 .. 1907) NdeI (1895) EcoRI (2089) XbaI * (2093) cPPT/CTS PaeR7I - PspXI - XhoI (2290) AgeI (2375) EF-1α core promoter BamHI (2541) Kozak sequence EGFP-N (2625 .. 2646) EXFP-R (2886 .. 2905) EGFP-C (3233 .. 3254) BsrGI (3289) BmgBI (3341) Neo-R (3417 .. 3436) PstI (3544) MscI (3573) PflFI - Tth111I (3609) NeoR/KanR RsrII (4007) Neo-F (4027 .. 4046) LRG-2.1T-neo-sgROSA 8307 bp

Sequence View

5ʹ 3ʹ 60 RSV promoter t t a a t g t a g t c t t a t g c a a t a c t c t t g t a g t c t t g c a a c a t g g t a a c g a t g a g t t a g c a a a a t t a c a t c a g a a t a c g t t a t g a g a a c a t c a g a a c g t t g t a c c a t t g c t a c t c a a t c g t t

120 RSV promoter c a t g c c t t a c a a g g a g a g a a a a a g c a c c g t g c a t g c c g a t t g g t g g a a g t a a g g t g g t a c g t a c g g a a t g t t c c t c t c t t t t t c g t g g c a c g t a c g g c t a a c c a c c t t c a t t c c a c c a t g

180 RSV promoter g a t c g t g c c t t a t t a g g a a g g c a a c a g a c g g g t c t g a c a t g g a t t g g a c g a a c c a c t g a a c t a g c a c g g a a t a a t c c t t c c g t t g t c t g c c c a g a c t g t a c c t a a c c t g c t t g g t g a c t t

240 RSV promoter 5' LTR (truncated) t t g c c g c a t t g c a g a g a t a t t g t a t t t a a g t g c c t a g c t c g a t a c a t a a a c g g g t c t c t c a a c g g c g t a a c g t c t c t a t a a c a t a a a t t c a c g g a t c g a g c t a t g t a t t t g c c c a g a g a g

300 5' LTR (truncated) t g g t t a g a c c a g a t c t g a g c c t g g g a g c t c t c t g g c t a a c t a g g g a a c c c a c t g c t t a a g a c c a a t c t g g t c t a g a c t c g g a c c c t c g a g a g a c c g a t t g a t c c c t t g g g t g a c g a a t t c

360 5' LTR (truncated) c c t c a a t a a a g c t t g c c t t g a g t g c t t c a a g t a g t g t g t g c c c g t c t g t t g t g t g a c t c t g g a g t t a t t t c g a a c g g a a c t c a c g a a g t t c a t c a c a c a c g g g c a g a c a a c a c a c t g a g a

420 5' LTR (truncated) g g t a a c t a g a g a t c c c t c a g a c c c t t t t a g t c a g t g t g g a a a a t c t c t a g c a g t g g c g c c c c a t t g a t c t c t a g g g a g t c t g g g a a a a t c a g t c a c a c c t t t t a g a g a t c g t c a c c g c g g

480 HIV-1 Ψ c g a a c a g g g a c t t g a a a g c g a a a g g g a a a c c a g a g g a g c t c t c t c g a c g c a g g a c t c g g c g c t t g t c c c t g a a c t t t c g c t t t c c c t t t g g t c t c c t c g a g a g a g c t g c g t c c t g a g c c g

540 HIV-1 Ψ t t g c t g a a g c g c g c a c g g c a a g a g g c g a g g g g c g g c g a c t g g t g a g t a c g c c a a a a a t t t a a c g a c t t c g c g c g t g c c g t t c t c c g c t c c c c g c c g c t g a c c a c t c a t g c g g t t t t t a a a

600 HIV-1 Ψ t g a c t a g c g g a g g c t a g a a g g a g a g a g a t g g g t g c g a g a g c g t c a g t a t t a a g c g g g g g a a c t g a t c g c c t c c g a t c t t c c t c t c t c t a c c c a c g c t c t c g c a g t c a t a a t t c g c c c c c t

NruI* 660 g a a t t a g a t c g c g a t g g g a a a a a a t t c g g t t a a g g c c a g g g g g a a a g a a a a a a t a t a a a t c t t a a t c t a g c g c t a c c c t t t t t t a a g c c a a t t c c g g t c c c c c t t t c t t t t t t a t a t t t a

720 t a a a a c a t a t a g t a t g g g c a a g c a g g g a g c t a g a a c g a t t c g c a g t t a a t c c t g g c c t g t a t t t t g t a t a t c a t a c c c g t t c g t c c c t c g a t c t t g c t a a g c g t c a a t t a g g a c c g g a c a

780 t a g a a a c a t c a g a a g g c t g t a g a c a a a t a c t g g g a c a g c t a c a a c c a t c c c t t c a g a c a g a t c t t t g t a g t c t t c c g a c a t c t g t t t a t g a c c c t g t c g a t g t t g g t a g g g a a g t c t g t c

840 g a t c a g a a g a a c t t a g a t c a t t a t a t a a t a c a g t a g c a a c c c t c t a t t g t g t g c a t c a a a c t a g t c t t c t t g a a t c t a g t a a t a t a t t a t g t c a t c g t t g g g a g a t a a c a c a c g t a g t t t

900 g g a t a g a g a t a a a a g a c a c c a a g g a a g c t t t a g a c a a g a t a g a g g a a g a g c a a a a c a a a a c c t a t c t c t a t t t t c t g t g g t t c c t t c g a a a t c t g t t c t a t c t c c t t c t c g t t t t g t t t t

NotI 960 MR g t a a g a c c a c c g c a c a g c a a g c g g c c g c t g a t c t t c a g a c c t g g a g g a g g a g a t a t g a g g c a t t c t g g t g g c g t g t c g t t c g c c g g c g a c t a g a a g t c t g g a c c t c c t c c t c t a t a c t c c

1020 DNWRSELYKYKVVKIEPLGV g a c a a t t g g a g a a g t g a a t t a t a t a a a t a t a a a g t a g t a a a a a t t g a a c c a t t a g g a g t a c t g t t a a c c t c t t c a c t t a a t a t a t t t a t a t t t c a t c a t t t t t a a c t t g g t a a t c c t c a t

1080 RRE APTKAKRRVVQREKRAVGIG g c a c c c a c c a a g g c a a a g a g a a g a g t g g t g c a g a g a g a a a a a a g a g c a g t g g g a a t a g g a c g t g g g t g g t t c c g t t t c t c t t c t c a c c a c g t c t c t c t t t t t t c t c g t c a c c c t t a t c c t

1140 RRE ALFLGFLGAAGSTMGAASMT g c t t t g t t c c t t g g g t t c t t g g g a g c a g c a g g a a g c a c t a t g g g c g c a g c g t c a a t g a c g c g a a a c a a g g a a c c c a a g a a c c c t c g t c g t c c t t c g t g a t a c c c g c g t c g c a g t t a c t g c

BbvCI 1200 RRE LTVQARQLLSGIVQQQNNLL c t g a c g g t a c a g g c c a g a c a a t t a t t g t c t g g t a t a g t g c a g c a g c a g a a c a a t t t g c t g g a c t g c c a t g t c c g g t c t g t t a a t a a c a g a c c a t a t c a c g t c g t c g t c t t g t t a a a c g a c

BstAPI 1260 RRE RAIEAQQHLLQLTVWGIKQL a g g g c t a t t g a g g c g c a a c a g c a t c t g t t g c a a c t c a c a g t c t g g g g c a t c a a g c a g c t c t c c c g a t a a c t c c g c g t t g t c g t a g a c a a c g t t g a g t g t c a g a c c c c g t a g t t c g t c g a g

1320 RRE QARILAVERYLKDQQLLGIW c a g g c a a g a a t c c t g g c t g t g g a a a g a t a c c t a a a g g a t c a a c a g c t c c t g g g g a t t t g g g t c c g t t c t t a g g a c c g a c a c c t t t c t a t g g a t t t c c t a g t t g t c g a g g a c c c c t a a a c c

1380 GCSGKLICTTAVPWNASWSN g g t t g c t c t g g a a a a c t c a t t t g c a c c a c t g c t g t g c c t t g g a a t g c t a g t t g g a g t a a t c c a a c g a g a c c t t t t g a g t a a a c g t g g t g a c g a c a c g g a a c c t t a c g a t c a a c c t c a t t a

1440 KSLEQIWNHTTWMEWDREIN a a a t c t c t g g a a c a g a t t t g g a a t c a c a c g a c c t g g a t g g a g t g g g a c a g a g a a a t t a a c t t t a g a g a c c t t g t c t a a a c c t t a g t g t g c t g g a c c t a c c t c a c c c t g t c t c t t t a a t t g

1500 1 KN gp41 peptide NYTSLIHSLIEESQNQQEKN a a t t a c a c a a g c t t a a t a c a c t c c t t a a t t g a a g a a t c g c a a a a c c a g c a a g a a a a g a a t t t a a t g t g t t c g a a t t a t g t g a g g a a t t a a c t t c t t a g c g t t t t g g t c g t t c t t t t c t t a

1560 5 10 15 EQELLELDKWASL (in frame with gp41 peptide) WNWFNIT gp41 peptide EQELLELDKWASLWNWFNIT g a a c a a g a a t t a t t g g a a t t a g a t a a a t g g g c a a g t t t g t g g a a t t g g t t t a a c a t a a c a c t t g t t c t t a a t a a c c t t a a t c t a t t t a c c c g t t c a a a c a c c t t a a c c a a a t t g t a t t g t

1620 (in frame with gp41 peptide) NWLWYIKLFIMIVGGLVGLR NWLWYIKLFIMIVGGLVGLR a a t t g g c t g t g g t a t a t a a a a t t a t t c a t a a t g a t a g t a g g a g g c t t g g t a g g t t t a a g a t t a a c c g a c a c c a t a t a t t t t a a t a a g t a t t a c t a t c a t c c t c c g a a c c a t c c a a a t t c t

1680 (in frame with gp41 peptide) IVFAVLSIVNRVRQGYSPLS IVFAVLSIVNRVRQGYSPLS a t a g t t t t t g c t g t a c t t t c t a t a g t g a a t a g a g t t a g g c a g g g a t a t t c a c c a t t a t c g t a t c a a a a a c g a c a t g a a a g a t a t c a c t t a t c t c a a t c c g t c c c t a t a a g t g g t a a t a g c

PpuMIKflI 1740 (in frame with gp41 peptide) FQTHLPTPRGPREGLFPMIP U6 promoter G A G G G C C T A T T T C C C A T G A T T hU6-F FQTHLPTPRGPREGLFPMIP t t t c a g a c c c a c c t c c c a a c c c c g a g g g g a c c c a g a g a g g g c c t a t t t c c c a t g a t t c c t a a a g t c t g g g t g g a g g g t t g g g g c t c c c c t g g g t c t c t c c c g g a t a a a g g g t a c t a a g g a

1800 (in frame with gp41 peptide) SYLHIRYKAVREIIRINLTV U6 promoter SYLHIRYKAVREIIRINLTV t c a t a t t t g c a t a t a c g a t a c a a g g c t g t t a g a g a g a t a a t t a g a a t t a a t t t g a c t g t a a g t a t a a a c g t a t a t g c t a t g t t c c g a c a a t c t c t c t a t t a a t c t t a a t t a a a c t g a c a t

1860 (in frame with gp41 peptide) NTKILVQNT* U6 promoter NTKILVQNT* a a c a c a a a g a t a t t a g t a c a a a a t a c g t g a c g t a g a a a g t a a t a a t t t c t t g g g t a g t t t t t g t g t t t c t a t a a t c a t g t t t t a t g c a c t g c a t c t t t c a t t a t t a a a g a a c c c a t c a a a

NdeI 1920 U6 promoter G A C T A T C A T A T G C T T A C C G T LKO.1 5' g c a g t t t t a a a a t t a t g t t t t a a a a t g g a c t a t c a t a t g c t t a c c g t a a c t t g a a a g t a t c g t c a a a a t t t t a a t a c a a a a t t t t a c c t g a t a g t a t a c g a a t g g c a t t g a a c t t t c a t a

1980 U6 promoter t t c g a t t t c t t g g c t t t a t a t a t c t t g t g g a a a g g a c g a a a c a c c g a a g a t g g g c g g g a g a a g c t a a a g a a c c g a a a t a t a t a g a a c a c c t t t c c t g c t t t g t g g c t t c t a c c c g c c c t c

2040 t c t t c g t t t a a g a g c t a t g c t g g a a a c a g c a t a g c a a g t t t a a a t a a g g c t a g t c c g t t a a g a a g c a a a t t c t c g a t a c g a c c t t t g t c g t a t c g t t c a a a t t t a t t c c g a t c a g g c a a t

EcoRIXbaI* 2100 t c a a c t t t g c t g g a a a c a g c a a a g t g g c a c c g a g t c g g t g c t t t t t t t g a a t t c t a g a t c a g t t g a a a c g a c c t t t g t c g t t t c a c c g t g g c t c a g c c a c g a a a a a a a c t t a a g a t c t a g

2160 cPPT/CTS t t g a g a c a a a t g g c a g t a t t c a t c c a c a a t t t t a a a a g a a a a g g g g g g a t t g g g g g g t a c a a c t c t g t t t a c c g t c a t a a g t a g g t g t t a a a a t t t t c t t t t c c c c c c t a a c c c c c c a t g

2220 cPPT/CTS a g t g c a g g g g a a a g a a t a g t a g a c a t a a t a g c a a c a g a c a t a c a a a c t a a a g a a t t a c a a t c a c g t c c c c t t t c t t a t c a t c t g t a t t a t c g t t g t c t g t a t g t t t g a t t t c t t a a t g t t

2280 cPPT/CTS a a a c a a a t t a c a a a a a t t c a a a a t t t t c g g g t t t a t t a c a g g g a c a g c a g a g a t c c a c t t t t t g t t t a a t g t t t t t a a g t t t t a a a a g c c c a a a t a a t g t c c c t g t c g t c t c t a g g t g a a

PaeR7IPspXIXhoI 2340 EF-1 α core promoter t g g c g c c g g c t c g a g t g g c t c c g g t g c c c g t c a g t g g g c a g a g c g c a c a t c g c c c a c a g t a c c g c g g c c g a g c t c a c c g a g g c c a c g g g c a g t c a c c c g t c t c g c g t g t a g c g g g t g t c a

AgeI 2400 EF-1 α core promoter c c c c g a g a a g t t g g g g g g a g g g g t c g g c a a t t g a a c c g g t g c c t a g a g a a g g t g g c g c g g g g g g c t c t t c a a c c c c c c t c c c c a g c c g t t a a c t t g g c c a c g g a t c t c t t c c a c c g c g c c

2460 EF-1 α core promoter g g t a a a c t g g g a a a g t g a t g t c g t g t a c t g g c t c c g c c t t t t t c c c g a g g g t g g g g g a g a c c a t t t g a c c c t t t c a c t a c a g c a c a t g a c c g a g g c g g a a a a a g g g c t c c c a c c c c c t c t

2520 EF-1 α core promoter a c c g t a t a t a a g t g c a g t a g t c g c c g t g a a c g t t c t t t t t c g c a a c g g g t t t g c c g c c a g t g g c a t a t a t t c a c g t c a t c a g c g g c a c t t g c a a g a a a a a g c g t t g c c c a a a c g g c g g t c

BamHI 2580 EF-1 α core promoter Kozak sequence EGFP 1 5 DYKDDDDK FLAG EGFP MDYKDDDDK a a c a c a g g t g t c g t g a c g c g g g a t c c g c c a c c a t g g a t t a c a a a g a c g a t g a c g a t a a g a t t g t g t c c a c a g c a c t g c g c c c t a g g c g g t g g t a c c t a a t g t t t c t g c t a c t g c t a t t c t

2640 1 M 1a V 5 10 15 SKGEELFTGVVPILVELD EGFP 1 M 1a V 5 10 15 SKGEELFTGVVPILVELD EGFP G A C C A G C T C G A C C T G C EGFP-N MVSKGEELFTGVVPILVELD t g g t g a g c a a g g g c g a g g a g c t g t t c a c c g g g g t g g t g c c c a t c c t g g t c g a g c t g g a c g a c c a c t c g t t c c c g c t c c t c g a c a a g t g g c c c c a c c a c g g g t a g g a c c a g c t c g a c c t g c

2700 20 25 30 35 GDVNGHKFSVSGEGEGDATY EGFP 20 25 30 35 GDVNGHKFSVSGEGEGDATY EGFP C G C T G C EGFP-N GDVNGHKFSVSGEGEGDATY g c g a c g t a a a c g g c c a c a a g t t c a g c g t g t c c g g c g a g g g c g a g g g c g a t g c c a c c t a c g c g c t g c a t t t g c c g g t g t t c a a g t c g c a c a g g c c g c t c c c g c t c c c g c t a c g g t g g a t g c

2760 40 45 50 55 GKLTLKFICTTGKLPVPWPT EGFP 40 45 50 55 GKLTLKFICTTGKLPVPWPT EGFP GKLTLKFICTTGKLPVPWPT g c a a g c t g a c c c t g a a g t t c a t c t g c a c c a c c g g c a a g c t g c c c g t g c c c t g g c c c a c c c c g t t c g a c t g g g a c t t c a a g t a g a c g t g g t g g c c g t t c g a c g g g c a c g g g a c c g g g t g g g

2820 60 65 70 75 LVTTLTYGVQCFSRYPDHMK EGFP 60 65 70 75 LVTTLTYGVQCFSRYPDHMK EGFP LVTTLTYGVQCFSRYPDHMK t c g t g a c c a c c c t g a c c t a c g g c g t g c a g t g c t t c a g c c g c t a c c c c g a c c a c a t g a a g c a g c a c t g g t g g g a c t g g a t g c c g c a c g t c a c g a a g t c g g c g a t g g g g c t g g t g t a c t t c g

2880 80 85 90 95 QHDFFKSAMPEGYVQERTIF EGFP 80 85 90 95 QHDFFKSAMPEGYVQERTIF EGFP QHDFFKSAMPEGYVQERTIF a g c a c g a c t t c t t c a a g t c c g c c a t g c c c g a a g g c t a c g t c c a g g a g c g c a c c a t c t t c t t c g t g c t g a a g a a g t t c a g g c g g t a c g g g c t t c c g a t g c a g g t c c t c g c g t g g t a g a a g a

2940 100 105 110 115 FKDDGNYKTRAEVKFEGDTL EGFP 100 105 110 115 FKDDGNYKTRAEVKFEGDTL EGFP C T G C T G C C G T T G A T G T T C T G EXFP-R FKDDGNYKTRAEVKFEGDTL t c a a g g a c g a c g g c a a c t a c a a g a c c c g c g c c g a g g t g a a g t t c g a g g g c g a c a c c c t g g a g t t c c t g c t g c c g t t g a t g t t c t g g g c g c g g c t c c a c t t c a a g c t c c c g c t g t g g g a c c

3000 120 125 130 135 VNRIELKGIDFKEDGNILGH EGFP 120 125 130 135 VNRIELKGIDFKEDGNILGH EGFP VNRIELKGIDFKEDGNILGH t g a a c c g c a t c g a g c t g a a g g g c a t c g a c t t c a a g g a g g a c g g c a a c a t c c t g g g g c a c a a c t t g g c g t a g c t c g a c t t c c c g t a g c t g a a g t t c c t c c t g c c g t t g t a g g a c c c c g t g t

3060 140 145 150 155 KLEYNYNSHNVYIMADKQKN EGFP 140 145 150 155 KLEYNYNSHNVYIMADKQKN EGFP KLEYNYNSHNVYIMADKQKN a g c t g g a g t a c a a c t a c a a c a g c c a c a a c g t c t a t a t c a t g g c c g a c a a g c a g a a g a a c g t c g a c c t c a t g t t g a t g t t g t c g g t g t t g c a g a t a t a g t a c c g g c t g t t c g t c t t c t t g c

3120 160 165 170 175 GIKVNFKIRHNIEDGSVQLA EGFP 160 165 170 175 GIKVNFKIRHNIEDGSVQLA EGFP GIKVNFKIRHNIEDGSVQLA g c a t c a a g g t g a a c t t c a a g a t c c g c c a c a a c a t c g a g g a c g g c a g c g t g c a g c t c g c c g c g t a g t t c c a c t t g a a g t t c t a g g c g g t g t t g t a g c t c c t g c c g t c g c a c g t c g a g c g g c

3180 180 185 190 195 DHYQQNTPIGDGPVLLPDNH EGFP 180 185 190 195 DHYQQNTPIGDGPVLLPDNH EGFP DHYQQNTPIGDGPVLLPDNH a c c a c t a c c a g c a g a a c a c c c c c a t c g g c g a c g g c c c c g t g c t g c t g c c c g a c a a c c a c t t g g t g a t g g t c g t c t t g t g g g g g t a g c c g c t g c c g g g g c a c g a c g a c g g g c t g t t g g t g a

3240 200 205 210 215 YLSTQSALSKDPNEKRDHMV EGFP 200 205 210 215 YLSTQSALSKDPNEKRDHMV EGFP C A T G G T C C EGFP-C YLSTQSALSKDPNEKRDHMV a c c t g a g c a c c c a g t c c g c c c t g a g c a a a g a c c c c a a c g a g a a g c g c g a t c a c a t g g t c c t g g a c t c g t g g g t c a g g c g g g a c t c g t t t c t g g g g t t g c t c t t c g c g c t a g t g t a c c a g g

BsrGI 3300 220 225 230 235 LLEFVTAAGITLGMDELYK G EGFP 220 225 230 235 LLEFVTAAGITLGMDELYK G EGFP T G C T G G A G T T C G T G EGFP-C LLEFVTAAGITLGMDELYKG t g c t g g a g t t c g t g a c c g c c g c c g g g a t c a c t c t c g g c a t g g a c g a g c t g t a c a a g g g c a a c g a c c t c a a g c a c t g g c g g c g g c c c t a g t g a g a g c c g t a c c t g c t c g a c a t g t t c c c g t

BmgBI 3360 (in frame with EGFP) SGATNFSLLKQAGDVEENPG SG 1 5 10 15 ATNFSLLKQAGDVEENPG P2A SGATNFSLLKQAGDVEENPG g c g g c g c c a c c a a c t t c a g c c t g c t g a a g c a g g c c g g c g a c g t g g a g g a g a a c c c c g g c c c g c c g c g g t g g t t g a a g t c g g a c g a c t t c g t c c g g c c g c t g c a c c t c c t c t t g g g g c c g g

3420 (in frame with EGFP) PMLEQDGLHAGSPAAWVERL P 1 5 10 15 MLEQDGLHAGSPAAWVERL P2A NeoR/KanR G A T A Neo-R PMLEQDGLHAGSPAAWVERL c c a t g c t t g a a c a a g a t g g a t t g c a c g c a g g t t c t c c g g c c g c t t g g g t g g a g a g g c t a t g g t a c g a a c t t g t t c t a c c t a a c g t g c g t c c a a g a g g c c g g c g a a c c c a c c t c t c c g a t a

3480 (in frame with EGFP) FGYDWAQQTIGCSDAAVFRL 20 25 30 35 FGYDWAQQTIGCSDAAVFRL NeoR/KanR A G C C G A T A C T G A C C C G Neo-R FGYDWAQQTIGCSDAAVFRL t c g g c t a t g a c t g g g c a c a a c a g a c a a t c g g c t g c t c t g a t g c c g c c g t g t t c c g g c t g t a g c c g a t a c t g a c c c g t g t t g t c t g t t a g c c g a c g a g a c t a c g g c g g c a c a a g g c c g a c a

3540 (in frame with EGFP) SAQGRPVLFVKTDLSGALNE 40 45 50 55 SAQGRPVLFVKTDLSGALNE NeoR/KanR SAQGRPVLFVKTDLSGALNE c a g c g c a g g g g c g c c c g g t t c t t t t t g t c a a g a c c g a c c t g t c c g g t g c c c t g a a t g a a c g t c g c g t c c c c g c g g g c c a a g a a a a a c a g t t c t g g c t g g a c a g g c c a c g g g a c t t a c t t g

MscIPstI 3600 (in frame with EGFP) LQDEAARLSWLATTGVPCAA 60 65 70 75 LQDEAARLSWLATTGVPCAA NeoR/KanR LQDEAARLSWLATTGVPCAA t g c a g g a c g a g g c a g c g c g g c t a t c g t g g c t g g c c a c g a c g g g c g t t c c t t g c g c a g c t g a c g t c c t g c t c c g t c g c g c c g a t a g c a c c g a c c g g t g c t g c c c g c a a g g a a c g c g t c g a c

Tth111IPflFI 3660 (in frame with EGFP) VLDVVTEAGRDWLLLGEVPG 80 85 90 95 VLDVVTEAGRDWLLLGEVPG NeoR/KanR VLDVVTEAGRDWLLLGEVPG t g c t c g a c g t t g t c a c t g a a g c g g g a a g g g a c t g g c t g c t a t t g g g c g a a g t g c c g g g g c a c g a g c t g c a a c a g t g a c t t c g c c c t t c c c t g a c c g a c g a t a a c c c g c t t c a c g g c c c c g

3720 (in frame with EGFP) QDLLSSHLAPAEKVSIMADA 100 105 110 115 QDLLSSHLAPAEKVSIMADA NeoR/KanR QDLLSSHLAPAEKVSIMADA a g g a t c t c c t g t c a t c t c a c c t t g c t c c t g c c g a g a a a g t a t c c a t c a t g g c t g a t g c a a t c c t a g a g g a c a g t a g a g t g g a a c g a g g a c g g c t c t t t c a t a g g t a g t a c c g a c t a c g t t

3780 (in frame with EGFP) MRRLHTLDPATCPFDHQAKH 120 125 130 135 MRRLHTLDPATCPFDHQAKH NeoR/KanR MRRLHTLDPATCPFDHQAKH t g c g g c g g c t g c a t a c g c t t g a t c c g g c t a c c t g c c c a t t c g a c c a c c a a g c g a a a c a t c a c g c c g c c g a c g t a t g c g a a c t a g g c c g a t g g a c g g g t a a g c t g g t g g t t c g c t t t g t a g

3840 (in frame with EGFP) RIERARTRMEAGLVDQDDLD 140 145 150 155 RIERARTRMEAGLVDQDDLD NeoR/KanR RIERARTRMEAGLVDQDDLD g c a t c g a g c g a g c a c g t a c t c g g a t g g a a g c c g g t c t t g t c g a t c a g g a t g a t c t g g a c g c g t a g c t c g c t c g t g c a t g a g c c t a c c t t c g g c c a g a a c a g c t a g t c c t a c t a g a c c t g c

3900 (in frame with EGFP) EEHQGLAPAELFARLKARMP 160 165 170 175 EEHQGLAPAELFARLKARMP NeoR/KanR EEHQGLAPAELFARLKARMP a a g a g c a t c a g g g g c t c g c g c c a g c c g a a c t g t t c g c c a g g c t c a a g g c g c g c a t g c c c g t t c t c g t a g t c c c c g a g c g c g g t c g g c t t g a c a a g c g g t c c g a g t t c c g c g c g t a c g g g c

3960 (in frame with EGFP) DGEDLVVTHGDACLPNIMVE 180 185 190 195 DGEDLVVTHGDACLPNIMVE NeoR/KanR DGEDLVVTHGDACLPNIMVE a c g g c g a g g a t c t c g t c g t g a c c c a t g g c g a t g c c t g c t t g c c g a a t a t c a t g g t g g a a a t g c c g c t c c t a g a g c a g c a c t g g g t a c c g c t a c g g a c g a a c g g c t t a t a g t a c c a c c t t t

RsrII 4020 (in frame with EGFP) NGRFSGFIDCGRLGVADRYQ 200 205 210 215 NGRFSGFIDCGRLGVADRYQ NeoR/KanR NGRFSGFIDCGRLGVADRYQ a t g g c c g c t t t t c t g g a t t c a t c g a c t g t g g c c g g c t g g g t g t g g c g g a c c g c t a t c a g g t a c c g g c g a a a a g a c c t a a g t a g c t g a c a c c g g c c g a c c c a c a c c g c c t g g c g a t a g t c c

4080 (in frame with EGFP) DIALATRDIAEELGGEWADR 220 225 230 235 DIALATRDIAEELGGEWADR NeoR/KanR C G T T G G C T A C C C G T G A T A T T Neo-F DIALATRDIAEELGGEWADR a c a t a g c g t t g g c t a c c c g t g a t a t t g c t g a a g a g c t t g g c g g c g a a t g g g c t g a c c g c t t g t a t c g c a a c c g a t g g g c a c t a t a a c g a c t t c t c g a a c c g c c g c t t a c c c g a c t g g c g a

4140 (in frame with EGFP) FLVLYGIAAPDSQRIAFYRL 240 245 250 255 FLVLYGIAAPDSQRIAFYRL NeoR/KanR FLVLYGIAAPDSQRIAFYRL t c c t c g t g c t t t a c g g t a t c g c c g c t c c c g a t t c g c a g c g c a t c g c c t t c t a t c g c c t t c a g g a g c a c g a a a t g c c a t a g c g g c g a g g g c t a a g c g t c g c g t a g c g g a a g a t a g c g g a a g

4200 (in frame with EGFP) LDEFF* WPRE 260 265 LDEFF* NeoR/KanR LDEFF* t t g a c g a g t t c t t c t g a g t c g a c a a t c a a c c t c t g g a t t a c a a a a t t t g t g a a a g a t t g a a a c t g c t c a a g a a g a c t c a g c t g t t a g t t g g a g a c c t a a t g t t t t a a a c a c t t t c t a a c t

4260 WPRE A C A A C G A G G A A A A T G C G A T A C WPRE-R MP c t g g t a t t c t t a a c t a t g t t g c t c c t t t t a c g c t a t g t g g a t a c g c t g c t t t a a t g c c t t g a c c a t a a g a a t t g a t a c a a c g a g g a a a a t g c g a t a c a c c t a t g c g a c g a a a t t a c g g a a

4320 WPRE MLLLPVWLSFSPPCINPG LYHAIASRMAFIFSSLYKSW t g t a t c a t g c t a t t g c t t c c c g t a t g g c t t t c a t t t t c t c c t c c t t g t a t a a a t c c t g g t a c a t a g t a c g a t a a c g a a g g g c a t a c c g a a a g t a a a a g a g g a g g a a c a t a t t t a g g a c c a

4380 WPRE CCLFMRSCGPLSGNVAWCAL LLSLYEELWPVVRQRGVVCT t g c t g t c t c t t t a t g a g g a g t t g t g g c c c g t t g t c a g g c a a c g t g g c g t g g t g t g c a c t g a c g a c a g a g a a a t a c t c c t c a a c a c c g g g c a a c a g t c c g t t g c a c c g c a c c a c a c g t g a c

PflMI 4440 WPRE CLLTQPPLVGALPPPVSSFP VFADATPTGWGIATTCQLLS t g t t t g c t g a c g c a a c c c c c a c t g g t t g g g g c a t t g c c a c c a c c t g t c a g c t c c t t t c c g a c a a a c g a c t g c g t t g g g g g t g a c c a a c c c c g t a a c g g t g g t g g a c a g t c g a g g a a a g g c

4500 WPRE GLSLSPSLLPRRNSSPPALP GTFAFPLPIATAELIAACLA g g a c t t t c g c t t t c c c c c t c c c t a t t g c c a c g g c g g a a c t c a t c g c c g c c t g c c t t g c c c c c t g a a a g c g a a a g g g g g a g g g a t a a c g g t g c c g c c t t g a g t a g c g g c g g a c g g a a c g g g

4560 WPRE AAGQGLGCWALTIPWCCRGN RCWTGARLLGTDNSVVLSGK g c t g c t g g a c a g g g g c t c g g c t g t t g g g c a c t g a c a a t t c c g t g g t g t t g t c g g g g a a a t c g a c g a c c t g t c c c c g a g c c g a c a a c c c g t g a c t g t t a a g g c a c c a c a a c a g c c c c t t t a

ZraIAatII 4620 WPRE (in frame with Factor Xa site) *RGKRPQEGTNGGPNQAPRG HRPFLGCSPVLPPGFCAGRP SSSFPWLLACVATWILRGTS c a t c g t c c t t t c c t t g g c t g c t c g c c t g t g t t g c c a c c t g g a t t c t g c g c g g g a c g t c c t g t a g c a g g a a a g g a a c c g a c g a g c g g a c a c a a c g g t g g a c c t a a g a c g c g c c c t g c a g g a

SacII 4680 WPRE EAVDR 1 RGEI Factor Xa site SATSLRPSIQRTFLPAACCR FCYVPSALNPADLPSRGLLP t c t g c t a c g t c c c t t c g g c c c t c a a t c c a g c g g a c c t t c c t t c c c g c g g c c t g c t g c c g g a g a c g a t g c a g g g a a g c c g g g a g t t a g g t c g c c t g g a a g g a a g g g c g c c g g a c g a c g g c c

4740 WPRE LCGLFRVFAFALRRVGSPFG ALRPLPRLRLRPQTSRISLW c t c t g c g g c c t c t t c c g c g t c t t c g c c t t c g c c c t c a g a c g a g t c g g a t c t c c c t t t g g g g a g a c g c c g g a g a a g g c g c a g a a g c g g a a g c g g g a g t c t g c t c a g c c t a g a g g g a a a c c c

4800 WPRE PPPRVDFKTNDLQGSCRS* AASPRRL* c c g c c t c c c c g c g t c g a c t t t a a g a c c a a t g a c t t a c a a g g c a g c t g t a g a t c t t a g c c a g g c g g a g g g g c g c a g c t g a a a t t c t g g t t a c t g a a t g t t c c g t c g a c a t c t a g a a t c g g t

4860 3' LTR ( Δ U3) c t t t t t a a a a g a a a a g g g g g g a c t g g a a g g g c t a a t t c a c t c c c a a c g a a g a c a a g a t c t g a a a a a t t t t c t t t t c c c c c c t g a c c t t c c c g a t t a a g t g a g g g t t g c t t c t g t t c t a g a

4920 3' LTR ( Δ U3) g c t t t t t g c t t g t a c t g g g t c t c t c t g g t t a g a c c a g a t c t g a g c c t g g g a g c t c t c t g g c g a a a a a c g a a c a t g a c c c a g a g a g a c c a a t c t g g t c t a g a c t c g g a c c c t c g a g a g a c c

4980 3' LTR ( Δ U3) c t a a c t a g g g a a c c c a c t g c t t a a g c c t c a a t a a a g c t t g c c t t g a g t g c t t c a a g t a g t g a t t g a t c c c t t g g g t g a c g a a t t c g g a g t t a t t t c g a a c g g a a c t c a c g a a g t t c a t c a

5040 3' LTR ( Δ U3) g t g t g c c c g t c t g t t g t g t g a c t c t g g t a a c t a g a g a t c c c t c a g a c c c t t t t a g t c a g t c a c a c g g g c a g a c a a c a c a c t g a g a c c a t t g a t c t c t a g g g a g t c t g g g a a a a t c a g t c a

SnaBI 5100 3' LTR ( Δ U3) g t g g a a a a t c t c t a g c a g t a c g t a t a g t a g t t c a t g t c a t c t t a t t a t t c a g t a t t t a t a c a c c t t t t a g a g a t c g t c a t g c a t a t c a t c a a g t a c a g t a g a a t a a t a a g t c a t a a a t a t

5160 SV40 poly(A) signal a c t t g c a a a g a a a t g a a t a t c a g a g a g t g a g a g g a a c t t g t t t a t t g c a g c t t a t a a t g g t g a a c g t t t c t t t a c t t a t a g t c t c t c a c t c t c c t t g a a c a a a t a a c g t c g a a t a t t a c c

5220 SV40 poly(A) signal C G T T A T C G T A G T G T T T A A A G SV40pA-R t t a c a a a t a a a g c a a t a g c a t c a c a a a t t t c a c a a a t a a a g c a t t t t t t t c a c t g c a t t c a a t g t t t a t t t c g t t a t c g t a g t g t t t a a a g t g t t t a t t t c g t a a a a a a a g t g a c g t a a g

5280 SV40 poly(A) signal G T G G T T T G T C C A A A C T C A T C EBV-rev t a g t t g t g g t t t g t c c a a a c t c a t c a a t g t a t c t t a t c a t g t c t g g c t c t a g c t a t c c c g a t c a a c a c c a a a c a g g t t t g a g t a g t t a c a t a g a a t a g t a c a g a c c g a g a t c g a t a g g g c

5340 SV40 ori c c c c t a a c t c c g c c c a t c c c g c c c c t a a c t c c g c c c a g t t c c g c c c a t t c t c c g c c c c a t g g g g a t t g a g g c g g g t a g g g c g g g g a t t g a g g c g g g t c a a g g c g g g t a a g a g g c g g g g t a

SfiI 5400 SV40 ori g g c t g a c t a a t t t t t t t t a t t t a t g c a g a g g c c g a g g c c g c c t c g g c c t c t g a g c t a t t c c c g a c t g a t t a a a a a a a a t a a a t a c g t c t c c g g c t c c g g c g g a g c c g g a g a c t c g a t a a g

StuIAvrII 5460 SV40 ori T7 promoter G G G A T A T C A C T7 c a g a a g t a g t g a g g a g g c t t t t t t g g a g g c c t a g g g a c g t a c c c a a t t c g c c c t a t a g t g g t c t t c a t c a c t c c t c c g a a a a a a c c t c c g g a t c c c t g c a t g g g t t a a g c g g g a t a t c a c

5520 T7 promoter M13 fwd T C A G C A T A A T T7 T G A C C G G C A G C A A A A T G T M13 Forward G C A A A A T G T T G C A G C A C T G A C C C M13/pUC Forward a g t c g t a t t a c g c g c g c t c a c t g g c c g t c g t t t t a c a a c g t c g t g a c t g g g a a a a c c c t g t c a g c a t a a t g c g c g c g a g t g a c c g g c a g c a a a a t g t t g c a g c a c t g a c c c t t t t g g g a c

5580 g c g t t a c c c a a c t t a a t c g c c t t g c a g c a c a t c c c c c t t t c g c c a g c t g g c g t a a t a g c g c g c a a t g g g t t g a a t t a g c g g a a c g t c g t g t a g g g g g a a a g c g g t c g a c c g c a t t a t c g c

5640 f1 ori a a g a g g c c c g c a c c g a t c g c c c t t c c c a a c a g t t g c g c a g c c t g a a t g g c g a a t g g g a c g t t c t c c g g g c g t g g c t a g c g g g a a g g g t t g t c a a c g c g t c g g a c t t a c c g c t t a c c c t g c

5700 f1 ori c g c c c t g t a g c g g c g c a t t a a g c g c g g c g g g t g t g g t g g t t a c g c g c a g c g t g a c c g c t a g c g g g a c a t c g c c g c g t a a t t c g c g c c g c c c a c a c c a c c a a t g c g c g t c g c a c t g g c g a t

5760 f1 ori G A G G A A A G C G A A A G A A G G G A F1ori-R c a c t t g c c a g c g c c c t a g c g c c c g c t c c t t t c g c t t t c t t c c c t t c c t t t c t c g c c a c g t g t g a a c g g t c g c g g g a t c g c g g g c g a g g a a a g c g a a a g a a g g g a a g g a a a g a g c g g t g c a

5820 f1 ori t c g c c g g c t t t c c c c g t c a a g c t c t a a a t c g g g g g c t c c c t t t a g g g t t c c g a t t t a g t g a g c g g c c g a a a g g g g c a g t t c g a g a t t t a g c c c c c g a g g g a a a t c c c a a g g c t a a a t c a c

DraIII 5880 f1 ori c t t t a c g g c a c c t c g a c c c c a a a a a a c t t g a t t a g g g t g a t g g t t c a c g t a g t g g g c c a t g a a a t g c c g t g g a g c t g g g g t t t t t t g a a c t a a t c c c a c t a c c a a g t g c a t c a c c c g g t a

5940 f1 ori G T G G A C F1ori-F c g c c c t g a t a g a c g g t t t t t c g c c c t t t g a c g t t g g a g t c c a c g t t c t t t a a t a g t g g a c g c g g g a c t a t c t g c c a a a a a g c g g g a a a c t g c a a c c t c a g g t g c a a g a a a t t a t c a c c t g

6000 f1 ori T C T T G T T C C A A A C T G G F1ori-F t c t t g t t c c a a a c t g g a a c a a c a c t c a a c c c t a t c t c g g t c t a t t c t t t t g a t t t a t a a g a g a a c a a g g t t t g a c c t t g t t g t g a g t t g g g a t a g a g c c a g a t a a g a a a a c t a a a t a t t c

6060 f1 ori g g a t t t t g c c g a t t t c g g c c t a t t g g t t a a a a a a t g a g c t g a t t t a a c a a a a a t t t a a c g c c t a a a a c g g c t a a a g c c g g a t a a c c a a t t t t t t a c t c g a c t a a a t t g t t t t t a a a t t g c

6120 f1 ori AmpR promoter c g a a t t t t a a c a a a a t a t t a a c g c t t a c a a t t t a g g t g g c a c t t t t c g g g g a a a t g t g c g g c t t a a a a t t g t t t t a t a a t t g c g a a t g t t a a a t c c a c c g t g a a a a g c c c c t t t a c a c g c

6180 AmpR promoter c g g a a c c c c t a t t t g t t t a t t t t t c t a a a t a c a t t c a a a t a t g t a t c c g c t c a t g a g a c a g c c t t g g g g a t a a a c a a a t a a a a a g a t t t a t g t a a g t t t a t a c a t a g g c g a g t a c t c t g t

6240 1 5 signal sequence MSIQHF AmpR promoter AmpR MSIQHF a t a a c c c t g a t a a a t g c t t c a a t a a t a t t g a a a a a g g a a g a g t a t g a g t a t t c a a c a t t t t a t t g g g a c t a t t t a c g a a g t t a t t a t a a c t t t t t c c t t c t c a t a c t c a t a a g t t g t a a a

6300 10 15 20 signal sequence RVALIPFFAAFCLPVFA 25 HPE AmpR RVALIPFFAAFCLPVFAHPE c c g t g t c g c c c t t a t t c c c t t t t t t g c g g c a t t t t g c c t t c c t g t t t t t g c t c a c c c a g a g g c a c a g c g g g a a t a a g g g a a a a a a c g c c g t a a a a c g g a a g g a c a a a a a c g a g t g g g t c t

6360 30 35 40 45 TLVKVKDAEDQLGARVGYIE AmpR TLVKVKDAEDQLGARVGYIE a a c g c t g g t g a a a g t a a a a g a t g c t g a a g a t c a g t t g g g t g c a c g a g t g g g t t a c a t c g a t t g c g a c c a c t t t c a t t t t c t a c g a c t t c t a g t c a a c c c a c g t g c t c a c c c a a t g t a g c t

6420 50 55 60 65 LDLNSGKILESFRPEERFPM AmpR LDLNSGKILESFRPEERFPM a c t g g a t c t c a a c a g c g g t a a g a t c c t t g a g a g t t t t c g c c c c g a a g a a c g t t t t c c a a t t g a c c t a g a g t t g t c g c c a t t c t a g g a a c t c t c a a a a g c g g g g c t t c t t g c a a a a g g t t a

6480 70 75 80 85 MSTFKVLLCGAVLSRIDAGQ AmpR C G A T A C A C C G C G C C A T A A T A Amp-R MSTFKVLLCGAVLSRIDAGQ g a t g a g c a c t t t t a a a g t t c t g c t a t g t g g c g c g g t a t t a t c c c g t a t t g a c g c c g g g c a c t a c t c g t g a a a a t t t c a a g a c g a t a c a c c g c g c c a t a a t a g g g c a t a a c t g c g g c c c g t

ScaI 6540 90 95 100 105 EQLGRRIHYSQNDLVEYSPV AmpR EQLGRRIHYSQNDLVEYSPV a g a g c a a c t c g g t c g c c g c a t a c a c t a t t c t c a g a a t g a c t t g g t t g a g t a c t c a c c a g t t c t c g t t g a g c c a g c g g c g t a t g t g a t a a g a g t c t t a c t g a a c c a a c t c a t g a g t g g t c a

6600 110 115 120 125 TEKHLTDGMTVRELCSAAIT AmpR TEKHLTDGMTVRELCSAAIT c a c a g a a a a g c a t c t t a c g g a t g g c a t g a c a g t a a g a g a a t t a t g c a g t g c t g c c a t a a c g t g t c t t t t c g t a g a a t g c c t a c c g t a c t g t c a t t c t c t t a a t a c g t c a c g a c g g t a t t g

6660 130 135 140 145 MSDNTAANLLLTTIGGPKEL AmpR MSDNTAANLLLTTIGGPKEL c a t g a g t g a t a a c a c t g c g g c c a a c t t a c t t c t g a c a a c g a t c g g a g g a c c g a a g g a g c t g t a c t c a c t a t t g t g a c g c c g g t t g a a t g a a g a c t g t t g c t a g c c t c c t g g c t t c c t c g a

6720 150 155 160 165 TAFLHNMGDHVTRLDRWEPE AmpR TAFLHNMGDHVTRLDRWEPE a a c c g c t t t t t t g c a c a a c a t g g g g g a t c a t g t a a c t c g c c t t g a t c g t t g g g a a c c g g a t t g g c g a a a a a a c g t g t t g t a c c c c c t a g t a c a t t g a g c g g a a c t a g c a a c c c t t g g c c t

6780 170 175 180 185 LNEAIPNDERDTTMPVAMAT AmpR LNEAIPNDERDTTMPVAMAT g c t g a a t g a a g c c a t a c c a a a c g a c g a g c g t g a c a c c a c g a t g c c t g t a g c a a t g g c a a c c g a c t t a c t t c g g t a t g g t t t g c t g c t c g c a c t g t g g t g c t a c g g a c a t c g t t a c c g t t g

6840 190 195 200 205 TLRKLLTGELLTLASRQQLI AmpR TLRKLLTGELLTLASRQQLI a a c g t t g c g c a a a c t a t t a a c t g g c g a a c t a c t t a c t c t a g c t t c c c g g c a a c a a t t a a t t t g c a a c g c g t t t g a t a a t t g a c c g c t t g a t g a a t g a g a t c g a a g g g c c g t t g t t a a t t a

6900 210 215 220 225 DWMEADKVAGPLLRSALPAG AmpR DWMEADKVAGPLLRSALPAG a g a c t g g a t g g a g g c g g a t a a a g t t g c a g g a c c a c t t c t g c g c t c g g c c c t t c c g g c t g g t c t g a c c t a c c t c c g c c t a t t t c a a c g t c c t g g t g a a g a c g c g a g c c g g g a a g g c c g a c c

6960 230 235 240 245 WFIADKSGAGERGSRGIIAA AmpR WFIADKSGAGERGSRGIIAA c t g g t t t a t t g c t g a t a a a t c t g g a g c c g g t g a g c g t g g g t c t c g c g g t a t c a t t g c a g c g a c c a a a t a a c g a c t a t t t a g a c c t c g g c c a c t c g c a c c c a g a g c g c c a t a g t a a c g t c g

AhdI 7020 250 255 260 265 LGPDGKPSRIVVIYTTGSQA AmpR LGPDGKPSRIVVIYTTGSQA a c t g g g g c c a g a t g g t a a g c c c t c c c g t a t c g t a g t t a t c t a c a c g a c g g g g a g t c a g g c t g a c c c c g g t c t a c c a t t c g g g a g g g c a t a g c a t c a a t a g a t g t g c t g c c c c t c a g t c c g

7080 270 275 280 285 TMDERNRQIAEIGASLIKHW AmpR TMDERNRQIAEIGASLIKHW a a c t a t g g a t g a a c g a a a t a g a c a g a t c g c t g a g a t a g g t g c c t c a c t g a t t a a g c a t t g t t g a t a c c t a c t t g c t t t a t c t g t c t a g c g a c t c t a t c c a c g g a g t g a c t a a t t c g t a a c

7140 * AmpR * g t a a c t g t c a g a c c a a g t t t a c t c a t a t a t a c t t t a g a t t g a t t t a a a a c t t c a t t t t t a c a t t g a c a g t c t g g t t c a a a t g a g t a t a t a t g a a a t c t a a c t a a a t t t t g a a g t a a a a a t

7200 a t t t a a a a g g a t c t a g g t g a a g a t c c t t t t t g a t a a t c t c a t g a c c a a a a t c c c t t a a c g t a a a t t t t c c t a g a t c c a c t t c t a g g a a a a a c t a t t a g a g t a c t g g t t t t a g g g a a t t g c

7260 ori t g a g t t t t c g t t c c a c t g a g c g t c a g a c c c c g t a g a a a a g a t c a a a g g a t c t t c t t g a g a a c t c a a a a g c a a g g t g a c t c g c a g t c t g g g g c a t c t t t t c t a g t t t c c t a g a a g a a c t c t

7320 ori t c c t t t t t t t c t g c g c g t a a t c t g c t g c t t g c a a a c a a a a a a a c c a c c g c t a c c a g c g g t a g g a a a a a a a g a c g c g c a t t a g a c g a c g a a c g t t t g t t t t t t t g g t g g c g a t g g t c g c c a

7380 ori g g t t t g t t t g c c g g a t c a a g a g c t a c c a a c t c t t t t t c c g a a g g t a a c t g g c t t c a g c a g c c a a a c a a a c g g c c t a g t t c t c g a t g g t t g a g a a a a a g g c t t c c a t t g a c c g a a g t c g t c

7440 ori a g c g c a g a t a c c a a a t a c t g t t c t t c t a g t g t a g c c g t a g t t a g g c c a c c a c t t c a a g a a t c g c g t c t a t g g t t t a t g a c a a g a a g a t c a c a t c g g c a t c a a t c c g g t g g t g a a g t t c t t

7500 ori c t c t g t a g c a c c g c c t a c a t a c c t c g c t c t g c t a a t c c t g t t a c c a g t g g c t g c t g c c a g g a g a c a t c g t g g c g g a t g t a t g g a g c g a g a c g a t t a g g a c a a t g g t c a c c g a c g a c g g t c

7560 ori t g g c g a t a a g t c g t g t c t t a c c g g g t t g g a c t c a a g a c g a t a g t t a c c g g a t a a g g c g c a a c c g c t a t t c a g c a c a g a a t g g c c c a a c c t g a g t t c t g c t a t c a a t g g c c t a t t c c g c g t

7620 ori g c g g t c g g g c t g a a c g g g g g g t t c g t g c a c a c a g c c c a g c t t g g a g c g a a c g a c c t a c a c c g c c a g c c c g a c t t g c c c c c c a a g c a c g t g t g t c g g g t c g a a c c t c g c t t g c t g g a t g t g

7680 ori c g a a c t g a g a t a c c t a c a g c g t g a g c t a t g a g a a a g c g c c a c g c t t c c c g a a g g g a g a a a g c t t g a c t c t a t g g a t g t c g c a c t c g a t a c t c t t t c g c g g t g c g a a g g g c t t c c c t c t t t

7740 ori g g c g g a c a g g t a t c c g g t a a g c g g c a g g g t c g g a a c a g g a g a g c g c a c g a g g g a g c t t c c c c g c c t g t c c a t a g g c c a t t c g c c g t c c c a g c c t t g t c c t c t c g c g t g c t c c c t c g a a g g

7800 ori G G G A A A C G C C T G G T A T C T T T pBR322ori-F a g g g g g a a a c g c c t g g t a t c t t t a t a g t c c t g t c g g g t t t c g c c a c c t c t g a c t t g a g c g t c c c c c t t t g c g g a c c a t a g a a a t a t c a g g a c a g c c c a a a g c g g t g g a g a c t g a a c t c g c

7860 ori t c g a t t t t t g t g a t g c t c g t c a g g g g g g c g g a g c c t a t g g a a a a a c g c c a g c a a c g c g g c a g c t a a a a a c a c t a c g a g c a g t c c c c c c g c c t c g g a t a c c t t t t t g c g g t c g t t g c g c c g

AflIIIPciI 7920 c t t t t t a c g g t t c c t g g c c t t t t g c t g g c c t t t t g c t c a c a t g t t c t t t c c t g c g t t a t c g a a a a a t g c c a a g g a c c g g a a a a c g a c c g g a a a a c g a g t g t a c a a g a a a g g a c g c a a t a g

7980 c c c t g a t t c t g t g g a t a a c c g t a t t a c c g c c t t t g a g t g a g c t g a t a c c g c t c g c c g c a g g g g a c t a a g a c a c c t a t t g g c a t a a t g g c g g a a a c t c a c t c g a c t a t g g c g a g c g g c g t c

8040 A G C G A G T C A G T G A G C G A G L4440 c c g a a c g a c c g a g c g c a g c g a g t c a g t g a g c g a g g a a g c g g a a g a g c g c c c a a t a c g c a a g g c t t g c t g g c t c g c g t c g c t c a g t c a c t c g c t c c t t c g c c t t c t c g c g g g t t a t g c g t t

8100 a c c g c c t c t c c c c g c g c g t t g g c c g a t t c a t t a a t g c a g c t g g c a c g a c a g g t t t c c c g a t g g c g g a g a g g g g c g c g c a a c c g g c t a a g t a a t t a c g t c g a c c g t g c t g t c c a a a g g g c t

8160 CAP binding site c t g g a a a g c g g g c a g t g a g c g c a a c g c a a t t a a t g t g a g t t a g c t c a c t c a t t a g g c a c c g a c c t t t c g c c c g t c a c t c g c g t t g c g t t a a t t a c a c t c a a t c g a g t g a g t a a t c c g t g g

8220 -35 -10 lac promoter lac operator A G C G G A T A A C A M13/pUC Reverse c c a g g c t t t a c a c t t t a t g c t t c c g g c t c g t a t g t t g t g t g g a a t t g t g a g c g g a t a a c a g g t c c g a a a t g t g a a a t a c g a a g g c c g a g c a t a c a a c a c a c c t t a a c a c t c g c c t a t t g t

8280 lac operator M13 rev T3 promoter A T T T C A C A C A G G M13/pUC Reverse C A G G A A A C A G C T A T G A C M13 Reverse G C A A T T A A C C C T C A C T A T3 a t t t c a c a c a g g a a a c a g c t a t g a c c a t g a t t a c g c c a a g c g c g c a a t t a a c c c t c a c t a t a a a g t g t g t c c t t t g t c g a t a c t g g t a c t a a t g c g g t t c g c g c g t t a a t t g g g a g t g a t

3ʹ 5ʹ 8307 T3 promoter A A G G T3 a a g g g a a c a a a a g c t g g a g c t g c a a g c t t c c c t t g t t t t c g a c c t c g a c g t t c g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.