Addgene pTet-O-Nd1-puro Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: pTet-O-Nd1-puro Sequencing Result


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LNCX (10,052 .. 10,076) minimal CMV promoter tetracycline response element AleI (9054) NotI (8625) 5' LTR (truncated) CMV promoter SnaBI (7693) NdeI (7587) SpeI (7352) MluI (7331) pRS-marker (7147 .. 7166) SgrDI (7101) AmpR promoter SspI (6984) Amp-R (6730 .. 6749) ScaI (6660) PvuI (6550) AhdI (6180) pBR322ori-F (5428 .. 5447) PciI (5287) L4440 (5177 .. 5194) EcoRI (1) BsaBI * (207) XbaI * (211) AscI (296) BfuAI - BspMI (393) XcmI (559) AfeI (649) BstXI (686) BlpI (845) Puro-R (1154 .. 1173) PasI (1203) BsiWI (1210) RsrII (1270) BstEII (1288) Puro-F (1650 .. 1670) PacI (1758) WPRE-R (1845 .. 1865) PflMI (2033) pBluescriptKS (2384 .. 2400) Bsu36I (2510) 5' LTR (truncated) PmeI (3095) BGH-rev (3111 .. 3128) F1ori-R (3474 .. 3493) F1ori-F (3684 .. 3705) pBABE 3' (3824 .. 3844) SV40pro-F (4071 .. 4090) AvrII (4143) EM7 promoter MauBI (4308) SgrAI (4386) FseI (4548) SV40pA-R (4813 .. 4832) SV40 poly(A) signal EBV-rev (4867 .. 4886) BstZ17I (4908) M13 Reverse (4946 .. 4962) lac operator M13/pUC Reverse (4959 .. 4981) lac promoter CAP binding site pTet-O-Nd1-puro 10,148 bp

Sequence View

EcoRI 60 MTKSYSESGLMGEPQ g a a t t c g c t a g c c a c c a t g a c c a a a t c a t a c a g c g a g a g c g g g c t g a t g g g c g a g c c t c a c t t a a g c g a t c g g t g g t a c t g g t t t a g t a t g t c g c t c t c g c c c g a c t a c c c g c t c g g a g t

120 PQGPPSWTDECLSSQDEEHE g c c c c a a g g t c c c c c a a g c t g g a c a g a t g a g t g t c t c a g t t c t c a g g a c g a g g a a c a c g a c g g g g t t c c a g g g g g t t c g a c c t g t c t a c t c a c a g a g t c a a g a g t c c t g c t c c t t g t g c t

180 ADKKEDELEAMNAEEDSLRN g g c a g a c a a g a a a g a g g a c g a g c t t g a a g c c a t g a a t g c a g a g g a g g a c t c t c t g a g a a a c c g t c t g t t c t t t c t c c t g c t c g a a c t t c g g t a c t t a c g t c t c c t c c t g a g a g a c t c t t t

BsaBI*XbaI* 240 GGEEEEEDEDLEEEEEEEEE c g g g g g a g a g g a g g a g g a g g a a g a t g a g g a t c t a g a g g a a g a g g a g g a a g a a g a a g a g g a g c c c c c t c t c c t c c t c c t c c t t c t a c t c c t a g a t c t c c t t c t c c t c c t t c t t c t t c t c c t

AscI 300 EEDQKPKRRGPKKKKMTKAR g g a g g a g g a t c a a a a g c c c a a g a g a c g g g g t c c c a a a a a g a a a a a g a t g a c c a a g g c g c g c c t c c t c c t a g t t t t c g g g t t c t c t g c c c c a g g g t t t t t c t t t t t c t a c t g g t t c c g c g c

360 LERFKLRRMKANARERNRMH c c t a g a a c g t t t t a a a t t a a g g c g c a t g a a g g c c a a c g c c c g c g a g c g g a a c c g c a t g c a g g a t c t t g c a a a a t t t a a t t c c g c g t a c t t c c g g t t g c g g g c g c t c g c c t t g g c g t a c g t

BspMIBfuAI 420 GLNAALDNLRKVVPCYSKTQ c g g g c t g a a c g c g g c g c t g g a c a a c c t g c g c a a g g t g g t a c c t t g c t a c t c c a a g a c c c a g c c c g a c t t g c g c c g c g a c c t g t t g g a c g c g t t c c a c c a t g g a a c g a t g a g g t t c t g g g t

480 KLSKIETLRLAKNYIWALSE g a a a c t g t c t a a a a t a g a g a c a c t g c g c t t g g c c a a g a a c t a c a t c t g g g c t c t g t c a g a c t t t g a c a g a t t t t a t c t c t g t g a c g c g a a c c g g t t c t t g a t g t a g a c c c g a g a c a g t c t

540 ILRSGKSPDLVSFVQTLCKG g a t c c t g c g c t c a g g c a a a a g c c c t g a t c t g g t c t c c t t c g t a c a g a c g c t c t g c a a a g g c t a g g a c g c g a g t c c g t t t t c g g g a c t a g a c c a g a g g a a g c a t g t c t g c g a g a c g t t t c c

XcmI 600 LSQPTTNLVAGCLQLNPRTF t t t g t c c c a g c c c a c t a c c a a t t t g g t c g c c g g c t g c c t g c a g c t c a a c c c t c g g a c t t t a a a c a g g g t c g g g t g a t g g t t a a a c c a g c g g c c g a c g g a c g t c g a g t t g g g a g c c t g a a a

AfeI 660 LPEQNPDMPPHLPTASASFP c t t g c c t g a g c a g a a c c c g g a c a t g c c c c c g c a t c t g c c a a c c g c c a g c g c t t c c t t c c c g a a c g g a c t c g t c t t g g g c c t g t a c g g g g g c g t a g a c g g t t g g c g g t c g c g a a g g a a g g g

BstXI 720 VHPYSYQSPGLPSPPYGTMD g g t g c a t c c c t a c t c c t a c c a g t c c c c t g g a c t g c c c a g c c c g c c c t a c g g c a c c a t g g a c c a c g t a g g g a t g a g g a t g g t c a g g g g a c c t g a c g g g t c g g g c g g g a t g c c g t g g t a c c t

780 SSHVFHVKPPPHAYSAALEP c a g c t c c c a c g t c t t c c a c g t c a a g c c g c c g c c a c a c g c c t a c a g c g c a g c t c t g g a g c c g t c g a g g g t g c a g a a g g t g c a g t t c g g c g g c g g t g t g c g g a t g t c g c g t c g a g a c c t c g g

840 FFESPLTDCTSPSFDGPLSP c t t c t t t g a a a g c c c c c t a a c t g a c t g c a c c a g c c c t t c c t t t g a c g g a c c c c t c a g c c c g a a g a a a c t t t c g g g g g a t t g a c t g a c g t g g t c g g g a a g g a a a c t g c c t g g g g a g t c g g g

BlpI 900 PLSINGNFSFKHEPSAEFEK g c c g c t c a g c a t c a a t g g c a a c t t c t c t t t c a a a c a c g a a c c a t c c g c c g a g t t t g a a a a c g g c g a g t c g t a g t t a c c g t t g a a g a g a a a g t t t g t g c t t g g t a g g c g g c t c a a a c t t t t

960 NYAFTMHYPAATLAGPQSHG a a a t t a t g c c t t t a c c a t g c a c t a c c c t g c a g c g a c g c t g g c a g g g c c c c a a a g c c a c g g t t t a a t a c g g a a a t g g t a c g t g a t g g g a c g t c g c t g c g a c c g t c c c g g g g t t t c g g t g c c

1020 SIFSSGAAAPRCEIPIDNIM a t c a a t c t t c t c t t c c g g t g c c g c t g c c c c t c g c t g c g a g a t c c c c a t a g a c a a c a t t a t t a g t t a g a a g a g a a g g c c a c g g c g a c g g g g a g c g a c g c t c t a g g g g t a t c t g t t g t a a t a

1080 SFDSHSHHERVMSAQLNAIF g t c t t t c g a t a g c c a t t c g c a t c a t g a g c g a g t c a t g a g t g c c c a g c t t a a t g c c a t c t t c a g a a a g c t a t c g g t a a g c g t a g t a c t c g c t c a g t a c t c a c g g g t c g a a t t a c g g t a g a a

1140 140 145 150 EGRGSLLTCGDVEENP T2A MRGRGGKS HDTREGRGSLLTCGDVEENP t c a c g a t a c t a g a g a g g g c a g g g g a a g t c t t c t a a c a t g c g g g g a c g t g g a g g a a a a t c c a g t g c t a t g a t c t c t c c c g t c c c c t t c a g a a g a t t g t a c g c c c c t g c a c c t c c t t t t a g g

1200 155 GP LE 1 5 10 15 MTEYKPTVRLATRDDV T2A PuroR T A C T G G C T C A T G T T C G G G T G Puro-R RPTRDDRVQAHGAPRHPRRR GPLEMTEYKPTVRLATRDDV c g g c c c a c t c g a g a t g a c c g a g t a c a a g c c c a c g g t g c g c c t c g c c a c c c g c g a c g a c g t g c c g g g t g a g c t c t a c t g g c t c a t g t t c g g g t g c c a c g c g g a g c g g t g g g c g c t g c t g c a

BsiWIPasI 1260 20 25 30 35 PRAVRTLAAAFADYPATRHT PuroR PQGRTHPRRRVRRLPRHAPH PRAVRTLAAAFADYPATRHT c c c c a g g g c c g t a c g c a c c c t c g c c g c c g c g t t c g c c g a c t a c c c c g c c a c g c g c c a c a c g g g g t c c c g g c a t g c g t g g g a g c g g c g g c g c a a g c g g c t g a t g g g g c g g t g c g c g g t g t g

BstEIIRsrII 1320 40 45 50 55 VDPDRHIERVTELQELFLTR PuroR RRSGPPHRAGHRAARTLPHA VDPDRHIERVTELQELFLTR c g t c g a t c c g g a c c g c c a c a t c g a g c g g g t c a c c g a g c t g c a a g a a c t c t t c c t c a c g c g g c a g c t a g g c c t g g c g g t g t a g c t c g c c c a g t g g c t c g a c g t t c t t g a g a a g g a g t g c g c

1380 60 65 70 75 VGLDIGKVWVADDGAAVAVW PuroR RRARHRQGVGRGRRRRGGGL VGLDIGKVWVADDGAAVAVW c g t c g g g c t c g a c a t c g g c a a g g t g t g g g t c g c g g a c g a c g g c g c c g c g g t g g c g g t c t g g c a g c c c g a g c t g t a g c c g t t c c a c a c c c a g c g c c t g c t g c c g c g g c g c c a c c g c c a g a c

1440 80 85 90 95 TTPESVEAGAVFAEIGPRMA PuroR DHAGERRSGGGVRRDRPAHG TTPESVEAGAVFAEIGPRMA g a c c a c g c c g g a g a g c g t c g a a g c g g g g g c g g t g t t c g c c g a g a t c g g c c c g c g c a t g g c c t g g t g c g g c c t c t c g c a g c t t c g c c c c c g c c a c a a g c g g c t c t a g c c g g g c g c g t a c c g

1500 100 105 110 115 ELSGSRLAAQQQMEGLLAPH PuroR RVERFPAGRAATDGRPPGAA ELSGSRLAAQQQMEGLLAPH c g a g t t g a g c g g t t c c c g g c t g g c c g c g c a g c a a c a g a t g g a a g g c c t c c t g g c g c c g c a g c t c a a c t c g c c a a g g g c c g a c c g g c g c g t c g t t g t c t a c c t t c c g g a g g a c c g c g g c g t

1560 120 125 130 135 RPKEPAWFLATVGVSPDHQG PuroR PAQGARVVPGHRRRLARPPG RPKEPAWFLATVGVSPDHQG c c g g c c c a a g g a g c c c g c g t g g t t c c t g g c c a c c g t c g g c g t c t c g c c c g a c c a c c a g g g g g c c g g g t t c c t c g g g c g c a c c a a g g a c c g g t g g c a g c c g c a g a g c g g g c t g g t g g t c c c

1620 140 145 150 155 KGLGSAVVLPGVEAAERAGV PuroR QGSGQRRRAPRSGGGRARRG KGLGSAVVLPGVEAAERAGV c a a g g g t c t g g g c a g c g c c g t c g t g c t c c c c g g a g t g g a g g c g g c c g a g c g c g c c g g g g t g t t c c c a g a c c c g t c g c g g c a g c a c g a g g g g c c t c a c c t c c g c c g g c t c g c g c g g c c c c a

1680 160 165 170 175 PAFLETSAPRNLPFYERLGF PuroR G C A A C C T C C C C T T C T A C G A G C Puro-F ARLPGDLRAPQPPLLRAARL PAFLETSAPRNLPFYERLGF g c c c g c c t t c c t g g a g a c c t c c g c g c c c c g c a a c c t c c c c t t c t a c g a g c g g c t c g g c t t c g g g c g g a a g g a c c t c t g g a g g c g c g g g g c g t t g g a g g g g a a g a t g c t c g c c g a g c c g a a

1740 180 185 190 195 TVTADVEVPEGPRTWCMTRK PuroR HRHRRRRGARRTAHLVHDPQ TVTADVEVPEGPRTWCMTRK c a c c g t c a c c g c c g a c g t c g a g g t g c c c g a a g g a c c g c g c a c c t g g t g c a t g a c c c g c a a g t g g c a g t g g c g g c t g c a g c t c c a c g g g c t t c c t g g c g c g t g g a c c a c g t a c t g g g c g t t

PacI 1800 200 PGA* PuroR WPRE ARCLIN* PGA* g c c c g g t g c c t g a t t a a t t a a g c t a g c t a a t t c g a t a t c a a g c t t a t c g a t a a t c a a c c t c g g g c c a c g g a c t a a t t a a t t c g a t c g a t t a a g c t a t a g t t c g a a t a g c t a t t a g t t g g a

1860 WPRE A C A A C G A G G A A A A T G C WPRE-R c t g g a t t a c a a a a t t t g t g a a a g a t t g a c t g g t a t t c t t a a c t a t g t t g c t c c t t t t a c g g a c c t a a t g t t t t a a a c a c t t t c t a a c t g a c c a t a a g a a t t g a t a c a a c g a g g a a a a t g c

1920 WPRE G A T A C WPRE-R MPLYHAIASRMAF MLLLPVWLS c t a t g t g g a t a c g c t g c t t t a a t g c c t t t g t a t c a t g c t a t t g c t t c c c g t a t g g c t t t c g a t a c a c c t a t g c g a c g a a a t t a c g g a a a c a t a g t a c g a t a a c g a a g g g c a t a c c g a a a g

1980 WPRE IFSSLYKSWLLSLYEELWPV FSPPCINPGCCLFMRSCGPL a t t t t c t c c t c c t t g t a t a a a t c c t g g t t g c t g t c t c t t t a t g a g g a g t t g t g g c c c g t t t a a a a g a g g a g g a a c a t a t t t a g g a c c a a c g a c a g a g a a a t a c t c c t c a a c a c c g g g c a a

PflMI 2040 WPRE VRQRGVVCTVFADATPTGWG SGNVAWCALCLLTQPPLVGA g t c a g g c a a c g t g g c g t g g t g t g c a c t g t g t t t g c t g a c g c a a c c c c c a c t g g t t g g g g c c a g t c c g t t g c a c c g c a c c a c a c g t g a c a c a a a c g a c t g c g t t g g g g g t g a c c a a c c c c g

2100 WPRE IATTCQLLSGTFAFPLPIAT LPPPVSSFPGLSLSPSLLPR a t t g c c a c c a c c t g t c a g c t c c t t t c c g g g a c t t t c g c t t t c c c c c t c c c t a t t g c c a c g t a a c g g t g g t g g a c a g t c g a g g a a a g g c c c t g a a a g c g a a a g g g g g a g g g a t a a c g g t g c

2160 WPRE AELIAACLARCWTGARLLGT RNSSPPALPAAGQGLGCWAL g c g g a a c t c a t c g c c g c c t g c c t t g c c c g c t g c t g g a c a g g g g c t c g g c t g t t g g g c a c t c g c c t t g a g t a g c g g c g g a c g g a a c g g g c g a c g a c c t g t c c c c g a g c c g a c a a c c c g t g a

2220 WPRE (in frame with Factor Xa site) *RGKRPQEGTN DNSVVLSGKSSSFPWLLACV TIPWCCRGNHRPFLGCSPVL g a c a a t t c c g t g g t g t t g t c g g g g a a a t c a t c g t c c t t t c c t t g g c t g c t c g c c t g t g t t c t g t t a a g g c a c c a c a a c a g c c c c t t t a g t a g c a g g a a a g g a a c c g a c g a g c g g a c a c a a

2280 WPRE (in frame with Factor Xa site) GGPNQAPRGEAVDR 1 RGEI Factor Xa site ATWILRGTSFCYVPSALNPA PPGFCAGRPSATSLRPSIQR g c c a c c t g g a t t c t g c g c g g g a c g t c c t t c t g c t a c g t c c c t t c g g c c c t c a a t c c a g c g c g g t g g a c c t a a g a c g c g c c c t g c a g g a a g a c g a t g c a g g g a a g c c g g g a g t t a g g t c g c

2340 WPRE DLPSRGLLPALRPLPRLRLR TFLPAACCRLCGLFRVFAFA g a c c t t c c t t c c c g c g g c c t g c t g c c g g c t c t g c g g c c t c t t c c g c g t c t t c g c c t t c g c c t g g a a g g a a g g g c g c c g g a c g a c g g c c g a g a c g c c g g a g a a g g c g c a g a a g c g g a a g c g

2400 WPRE KS primer C T A T G G C A G C T G G A G C T pBluescriptKS PQTSRISLWAASPHRYRRPR LRRVGSPFGPPPRIDTVDLE c c t c a g a c g a g t c g g a t c t c c c t t t g g g c c g c c t c c c c g c a t c g a t a c c g t c g a c c t c g a g g a g t c t g c t c a g c c t a g a g g g a a a c c c g g c g g a g g g g c g t a g c t a t g g c a g c t g g a g c t

2460 DLEKHGAITSSNTAATNADC T* g a c c t a g a a a a a c a t g g a g c a a t c a c a a g t a g c a a t a c a g c a g c t a c c a a t g c t g a t t g t c t g g a t c t t t t t g t a c c t c g t t a g t g t t c a t c g t t a t g t c g t c g a t g g t t a c g a c t a a c a

Bsu36I 2520 AWLEAQEEEEVGFPVTPQVP g c c t g g c t a g a a g c a c a a g a g g a g g a g g a g g t g g g t t t t c c a g t c a c a c c t c a g g t a c c t c g g a c c g a t c t t c g t g t t c t c c t c c t c c t c c a c c c a a a a g g t c a g t g t g g a g t c c a t g g a

2580 LRPMTYKAAVDLSHFLKEKG t t a a g a c c a a t g a c t t a c a a g g c a g c t g t a g a t c t t a g c c a c t t t t t a a a a g a a a a g g g g a a t t c t g g t t a c t g a a t g t t c c g t c g a c a t c t a g a a t c g g t g a a a a a t t t t c t t t t c c c c

2640 GLEGLIHSQRRQDILDLWIY g g a c t g g a a g g g c t a a t t c a c t c c c a a c g a a g a c a a g a t a t c c t t g a t c t g t g g a t c t a c c c t g a c c t t c c c g a t t a a g t g a g g g t t g c t t c t g t t c t a t a g g a a c t a g a c a c c t a g a t g

2700 HTQGYFPDWQNYTPGPGIRY c a c a c a c a a g g c t a c t t c c c t g a t t g g c a g a a c t a c a c a c c a g g g c c a g g g a t c a g a t a t g t g t g t g t t c c g a t g a a g g g a c t a a c c g t c t t g a t g t g t g g t c c c g g t c c c t a g t c t a t a

2760 PLTFGWCYKLVPVEQEKVEE c c a c t g a c c t t t g g a t g g t g c t a c a a g c t a g t a c c a g t t g a g c a a g a g a a g g t a g a a g a a g g t g a c t g g a a a c c t a c c a c g a t g t t c g a t c a t g g t c a a c t c g t t c t c t t c c a t c t t c t t

2820 ANEGENTRLLHPVSLHGMDD g c c a a t g a a g g a g a g a a c a c c c g c t t g t t a c a c c c t g t g a g c c t g c a t g g g a t g g a t g a c c g g t t a c t t c c t c t c t t g t g g g c g a a c a a t g t g g g a c a c t c g g a c g t a c c c t a c c t a c t g

2880 PEREVLEWRFDSRLAFHHMA c c g g a g a g a g a a g t a t t a g a g t g g a g g t t t g a c a g c c g c c t a g c a t t t c a t c a c a t g g c c g g c c t c t c t c t t c a t a a t c t c a c c t c c a a a c t g t c g g c g g a t c g t a a a g t a g t g t a c c g g

2940 5' LTR (truncated) RELHPDCTGSLWLDQI* c g a g a g c t g c a t c c g g a c t g t a c t g g g t c t c t c t g g t t a g a c c a g a t c t g a g c c t g g g a g g c t c t c g a c g t a g g c c t g a c a t g a c c c a g a g a g a c c a a t c t g g t c t a g a c t c g g a c c c t c

3000 5' LTR (truncated) c t c t c t g g c t a a c t a g g g a a c c c a c t g c t t a a g c c t c a a t a a a g c t t g c c t t g a g t g c t t g a g a g a c c g a t t g a t c c c t t g g g t g a c g a a t t c g g a g t t a t t t c g a a c g g a a c t c a c g a a

3060 5' LTR (truncated) c a a g t a g t g t g t g c c c g t c t g t t g t g t g a c t c t g g t a a c t a g a g a t c c c t c a g a c c c t t t g t t c a t c a c a c a c g g g c a g a c a a c a c a c t g a g a c c a t t g a t c t c t a g g g a g t c t g g g a a a

PmeI 3120 5' LTR (truncated) bGH poly(A) signal G G A G C T G A C A BGH-rev t a g t c a g t g t g g a a a a t c t c t a g c a g g g c c c g t t t a a a c c c g c t g a t c a g c c t c g a c t g t a t c a g t c a c a c c t t t t a g a g a t c g t c c c g g g c a a a t t t g g g c g a c t a g t c g g a g c t g a c a

3180 bGH poly(A) signal C G G A A G A T BGH-rev g c c t t c t a g t t g c c a g c c a t c t g t t g t t t g c c c c t c c c c c g t g c c t t c c t t g a c c c t g g a c g g a a g a t c a a c g g t c g g t a g a c a a c a a a c g g g g a g g g g g c a c g g a a g g a a c t g g g a c c t

3240 bGH poly(A) signal a g g t g c c a c t c c c a c t g t c c t t t c c t a a t a a a a t g a g g a a a t t g c a t c g c a t t g t c t g a g t c c a c g g t g a g g g t g a c a g g a a a g g a t t a t t t t a c t c c t t t a a c g t a g c g t a a c a g a c t c

3300 bGH poly(A) signal t a g g t g t c a t t c t a t t c t g g g g g g t g g g g t g g g g c a g g a c a g c a a g g g g g a g g a t t g g g a a t c c a c a g t a a g a t a a g a c c c c c c a c c c c a c c c c g t c c t g t c g t t c c c c c t c c t a a c c c t

3360 bGH poly(A) signal a g a c a a t a g c a g g c a t g c t g g g g a t g c g g t g g g c t c t a t g g c t t c t g a g g c g g a a a g a a c t c t g t t a t c g t c c g t a c g a c c c c t a c g c c a c c c g a g a t a c c g a a g a c t c c g c c t t t c t t g

3420 f1 ori c a g c t g g g g c t c t a g g g g g t a t c c c c a c g c g c c c t g t a g c g g c g c a t t a a g c g c g g c g g g g t c g a c c c c g a g a t c c c c c a t a g g g g t g c g c g g g a c a t c g c c g c g t a a t t c g c g c c g c c c

3480 f1 ori G A G G A A A F1ori-R t g t g g t g g t t a c g c g c a g c g t g a c c g c t a c a c t t g c c a g c g c c c t a g c g c c c g c t c c t t t a c a c c a c c a a t g c g c g t c g c a c t g g c g a t g t g a a c g g t c g c g g g a t c g c g g g c g a g g a a a

3540 f1 ori G C G A A A G A A G G G A F1ori-R c g c t t t c t t c c c t t c c t t t c t c g c c a c g t t c g c c g g c t t t c c c c g t c a a g c t c t a a a t c g g c g a a a g a a g g g a a g g a a a g a g c g g t g c a a g c g g c c g a a a g g g g c a g t t c g a g a t t t a g c

3600 f1 ori g g g g c t c c c t t t a g g g t t c c g a t t t a g t g c t t t a c g g c a c c t c g a c c c c a a a a a a c t t g a c c c c g a g g g a a a t c c c a a g g c t a a a t c a c g a a a t g c c g t g g a g c t g g g g t t t t t t g a a c t

3660 f1 ori t t a g g g t g a t g g t t c a c g t a g t g g g c c a t c g c c c t g a t a g a c g g t t t t t c g c c c t t t g a c a a t c c c a c t a c c a a g t g c a t c a c c c g g t a g c g g g a c t a t c t g c c a a a a a g c g g g a a a c t g

3720 f1 ori G T G G A C T C T T G T T C C A A A C T G G F1ori-F g t t g g a g t c c a c g t t c t t t a a t a g t g g a c t c t t g t t c c a a a c t g g a a c a a c a c t c a a c c c c a a c c t c a g g t g c a a g a a a t t a t c a c c t g a g a a c a a g g t t t g a c c t t g t t g t g a g t t g g g

3780 f1 ori t a t c t c g g t c t a t t c t t t t g a t t t a t a a g g g a t t t t g c c g a t t t c g g c c t a t t g g t t a a a a t a g a g c c a g a t a a g a a a a c t a a a t a t t c c c t a a a a c g g c t a a a g c c g g a t a a c c a a t t t

3840 f1 ori SV40 promoter C C T T A C A C A C A G T C A A T pBABE 3' a a a t g a g c t g a t t t a a c a a a a a t t t a a c g c g a a t t a a t t c t g t g g a a t g t g t g t c a g t t a t t t a c t c g a c t a a a t t g t t t t t a a a t t g c g c t t a a t t a a g a c a c c t t a c a c a c a g t c a a t

3900 SV40 promoter C C C A pBABE 3' g g g t g t g g a a a g t c c c c a g g c t c c c c a g c a g g c a g a a g t a t g c a a a g c a t g c a t c t c a a t c c c a c a c c t t t c a g g g g t c c g a g g g g t c g t c c g t c t t c a t a c g t t t c g t a c g t a g a g t t a

3960 SV40 promoter t a g t c a g c a a c c a g g t g t g g a a a g t c c c c a g g c t c c c c a g c a g g c a g a a g t a t g c a a a g c a t c a g t c g t t g g t c c a c a c c t t t c a g g g g t c c g a g g g g t c g t c c g t c t t c a t a c g t t t c g

4020 SV40 promoter SV40 ori a t g c a t c t c a a t t a g t c a g c a a c c a t a g t c c c g c c c c t a a c t c c g c c c a t c c c g c c c c t a t a c g t a g a g t t a a t c a g t c g t t g g t a t c a g g g c g g g g a t t g a g g c g g g t a g g g c g g g g a t

4080 SV40 promoter SV40 ori T A T T T A T G C A SV40pro-F a c t c c g c c c a g t t c c g c c c a t t c t c c g c c c c a t g g c t g a c t a a t t t t t t t t a t t t a t g c a t g a g g c g g g t c a a g g c g g g t a a g a g g c g g g g t a c c g a c t g a t t a a a a a a a a t a a a t a c g t

4140 SV40 promoter SV40 ori G A G G C C G A G G SV40pro-F g a g g c c g a g g c c g c c t c t g c c t c t g a g c t a t t c c a g a a g t a g t g a g g a g g c t t t t t t g g a c t c c g g c t c c g g c g g a g a c g g a g a c t c g a t a a g g t c t t c a t c a c t c c t c c g a a a a a a c c t

AvrII 4200 SV40 promoter SV40 ori g g c c t a g g c t t t t g c a a a a a g c t c c c g g g a g c t t g t a t a t c c a t t t t c g g a t c t g a t c a g c c g g a t c c g a a a a c g t t t t t c g a g g g c c c t c g a a c a t a t a g g t a a a a g c c t a g a c t a g t c

4260 EM7 promoter c a c g t g t t g a c a a t t a a t c a t c g g c a t a g t a t a t c g g c a t a g t a t a a t a c g a c a a g g t g a g t g c a c a a c t g t t a a t t a g t a g c c g t a t c a t a t a g c c g t a t c a t a t t a t g c t g t t c c a c t

MauBI 4320 1 5 10 15 MAKLTSAVPVLTARDV BleoR g g a a c t a a a c c a t g g c c a a g t t g a c c a g t g c c g t t c c g g t g c t c a c c g c g c g c g a c g t c g c c t t g a t t t g g t a c c g g t t c a a c t g g t c a c g g c a a g g c c a c g a g t g g c g c g c g c t g c a g c

4380 20 25 30 35 AGAVEFWTDRLGFSRDFVED BleoR c c g g a g c g g t c g a g t t c t g g a c c g a c c g g c t c g g g t t c t c c c g g g a c t t c g t g g a g g a c g g g c c t c g c c a g c t c a a g a c c t g g c t g g c c g a g c c c a a g a g g g c c c t g a a g c a c c t c c t g c

SgrAI 4440 40 45 50 55 DFAGVVRDDVTLFISAVQDQ BleoR a c t t c g c c g g t g t g g t c c g g g a c g a c g t g a c c c t g t t c a t c a g c g c g g t c c a g g a c c a g g t g a a g c g g c c a c a c c a g g c c c t g c t g c a c t g g g a c a a g t a g t c g c g c c a g g t c c t g g t c c

4500 60 65 70 75 VVPDNTLAWVWVRGLDELYA BleoR t g g t g c c g g a c a a c a c c c t g g c c t g g g t g t g g g t g c g c g g c c t g g a c g a g c t g t a c g c c g a c c a c g g c c t g t t g t g g g a c c g g a c c c a c a c c c a c g c g c c g g a c c t g c t c g a c a t g c g g c

FseI 4560 80 85 90 95 EWSEVVSTNFRDASGPAMTE BleoR a g t g g t c g g a g g t c g t g t c c a c g a a c t t c c g g g a c g c c t c c g g g c c g g c c a t g a c c g a g a t c a c c a g c c t c c a g c a c a g g t g c t t g a a g g c c c t g c g g a g g c c c g g c c g g t a c t g g c t c t

4620 100 105 110 115 IGEQPWGREFALRDPAGNCV BleoR t c g g c g a g c a g c c g t g g g g g c g g g a g t t c g c c c t g c g c g a c c c g g c c g g c a a c t g c g t g c a g c c g c t c g t c g g c a c c c c c g c c c t c a a g c g g g a c g c g c t g g g c c g g c c g t t g a c g c a c g

4680 120 125 HFVAEEQD* BleoR a c t t c g t g g c c g a g g a g c a g g a c t g a c a c g t g c t a c g a g a t t t c g a t t c c a c c g c c g c c t t g a a g c a c c g g c t c c t c g t c c t g a c t g t g c a c g a t g c t c t a a a g c t a a g g t g g c g g c g g a

4740 t c t a t g a a a g g t t g g g c t t c g g a a t c g t t t t c c g g g a c g c c g g c t g g a t g a t c c t c c a g c a g a t a c t t t c c a a c c c g a a g c c t t a g c a a a a g g c c c t g c g g c c g a c c t a c t a g g a g g t c g

4800 SV40 poly(A) signal g c g g g g a t c t c a t g c t g g a g t t c t t c g c c c a c c c c a a c t t g t t t a t t g c a g c t t a t a a t g c g c c c c t a g a g t a c g a c c t c a a g a a g c g g g t g g g g t t g a a c a a a t a a c g t c g a a t a t t a c

4860 SV40 poly(A) signal C G T T A T C G T A G T G T T T A A A G SV40pA-R g t t a c a a a t a a a g c a a t a g c a t c a c a a a t t t c a c a a a t a a a g c a t t t t t t t c a c t g c a t t c a a t g t t t a t t t c g t t a t c g t a g t g t t t a a a g t g t t t a t t t c g t a a a a a a a g t g a c g t a a

BstZ17I 4920 SV40 poly(A) signal G T G G T T T G T C C A A A C T C A T C EBV-rev c t a g t t g t g g t t t g t c c a a a c t c a t c a a t g t a t c t t a t c a t g t c t g t a t a c c g t c g a c c t g a t c a a c a c c a a a c a g g t t t g a g t a g t t a c a t a g a a t a g t a c a g a c a t a t g g c a g c t g g a

4980 M13 rev lac operator C A G T A T C G A C A A A G G A C M13 Reverse G G A C A C A C T T T A A C A A T A G G C G M13/pUC Reverse c t a g c t a g a g c t t g g c g t a a t c a t g g t c a t a g c t g t t t c c t g t g t g a a a t t g t t a t c c g c g a t c g a t c t c g a a c c g c a t t a g t a c c a g t a t c g a c a a a g g a c a c a c t t t a a c a a t a g g c g

5040 -10 -35 lac operator lac promoter CAP binding site A M13/pUC Reverse t c a c a a t t c c a c a c a a c a t a c g a g c c g g a a g c a t a a a g t g t a a a g c c t g g g g t g c c t a a t a g t g t t a a g g t g t g t t g t a t g c t c g g c c t t c g t a t t t c a c a t t t c g g a c c c c a c g g a t t a

5100 CAP binding site g a g t g a g c t a a c t c a c a t t a a t t g c g t t g c g c t c a c t g c c c g c t t t c c a g t c g g g a a a c c c t c a c t c g a t t g a g t g t a a t t a a c g c a a c g c g a g t g a c g g g c g a a a g g t c a g c c c t t t g g

5160 t g t c g t g c c a g c t g c a t t a a t g a a t c g g c c a a c g c g c g g g g a g a g g c g g t t t g c g t a t t g a c a g c a c g g t c g a c g t a a t t a c t t a g c c g g t t g c g c g c c c c t c t c c g c c a a a c g c a t a a c

5220 G A G C G A G T G A C T G A G C G A L4440 g g c g c t c t t c c g c t t c c t c g c t c a c t g a c t c g c t g c g c t c g g t c g t t c g g c t g c g g c g a g c c g c g a g a a g g c g a a g g a g c g a g t g a c t g a g c g a c g c g a g c c a g c a a g c c g a c g c c g c t c

5280 c g g t a t c a g c t c a c t c a a a g g c g g t a a t a c g g t t a t c c a c a g a a t c a g g g g a t a a c g c a g g c c a t a g t c g a g t g a g t t t c c g c c a t t a t g c c a a t a g g t g t c t t a g t c c c c t a t t g c g t c

PciI 5340 g a a a g a a c a t g t g a g c a a a a g g c c a g c a a a a g g c c a g g a a c c g t a a a a a g g c c g c g t t g c c t t t c t t g t a c a c t c g t t t t c c g g t c g t t t t c c g g t c c t t g g c a t t t t t c c g g c g c a a c g

5400 ori t g g c g t t t t t c c a t a g g c t c c g c c c c c c t g a c g a g c a t c a c a a a a a t c g a c g c t c a a g t c a c c g c a a a a a g g t a t c c g a g g c g g g g g g a c t g c t c g t a g t g t t t t t a g c t g c g a g t t c a g

5460 ori T T T C T A T G G T C C G C A A A G G G pBR322ori-F a g a g g t g g c g a a a c c c g a c a g g a c t a t a a a g a t a c c a g g c g t t t c c c c c t g g a a g c t c c c t c t c c a c c g c t t t g g g c t g t c c t g a t a t t t c t a t g g t c c g c a a a g g g g g a c c t t c g a g g g

5520 ori t c g t g c g c t c t c c t g t t c c g a c c c t g c c g c t t a c c g g a t a c c t g t c c g c c t t t c t c c c t t a g c a c g c g a g a g g a c a a g g c t g g g a c g g c g a a t g g c c t a t g g a c a g g c g g a a a g a g g g a a

5580 ori c g g g a a g c g t g g c g c t t t c t c a t a g c t c a c g c t g t a g g t a t c t c a g t t c g g t g t a g g t c g g c c c t t c g c a c c g c g a a a g a g t a t c g a g t g c g a c a t c c a t a g a g t c a a g c c a c a t c c a g c

5640 ori t t c g c t c c a a g c t g g g c t g t g t g c a c g a a c c c c c c g t t c a g c c c g a c c g c t g c g c c t t a t a a g c g a g g t t c g a c c c g a c a c a c g t g c t t g g g g g g c a a g t c g g g c t g g c g a c g c g g a a t a

5700 ori c c g g t a a c t a t c g t c t t g a g t c c a a c c c g g t a a g a c a c g a c t t a t c g c c a c t g g c a g c a g g g c c a t t g a t a g c a g a a c t c a g g t t g g g c c a t t c t g t g c t g a a t a g c g g t g a c c g t c g t c

5760 ori c c a c t g g t a a c a g g a t t a g c a g a g c g a g g t a t g t a g g c g g t g c t a c a g a g t t c t t g a a g t g g t g a c c a t t g t c c t a a t c g t c t c g c t c c a t a c a t c c g c c a c g a t g t c t c a a g a a c t t c a

5820 ori g g t g g c c t a a c t a c g g c t a c a c t a g a a g a a c a g t a t t t g g t a t c t g c g c t c t g c t g a a g c c c a c c g g a t t g a t g c c g a t g t g a t c t t c t t g t c a t a a a c c a t a g a c g c g a g a c g a c t t c g

5880 ori c a g t t a c c t t c g g a a a a a g a g t t g g t a g c t c t t g a t c c g g c a a a c a a a c c a c c g c t g g t a g t c a a t g g a a g c c t t t t t c t c a a c c a t c g a g a a c t a g g c c g t t t g t t t g g t g g c g a c c a t

5940 ori g c g g t g g t t t t t t t g t t t g c a a g c a g c a g a t t a c g c g c a g a a a a a a a g g a t c t c a a g a a g c g c c a c c a a a a a a a c a a a c g t t c g t c g t c t a a t g c g c g t c t t t t t t t c c t a g a g t t c t t c

6000 a t c c t t t g a t c t t t t c t a c g g g g t c t g a c g c t c a g t g g a a c g a a a a c t c a c g t t a a g g g a t a g g a a a c t a g a a a a g a t g c c c c a g a c t g c g a g t c a c c t t g c t t t t g a g t g c a a t t c c c t

6060 t t t t g g t c a t g a g a t t a t c a a a a a g g a t c t t c a c c t a g a t c c t t t t a a a t t a a a a a t g a a a a a a c c a g t a c t c t a a t a g t t t t t c c t a g a a g t g g a t c t a g g a a a a t t t a a t t t t t a c t t

6120 285 *WHKI AmpR *WHKI g t t t t a a a t c a a t c t a a a g t a t a t a t g a g t a a a c t t g g t c t g a c a g t t a c c a a t g c t t a a c a a a a t t t a g t t a g a t t t c a t a t a t a c t c a t t t g a a c c a g a c t g t c a a t g g t t a c g a a t t

AhdI 6180 280 275 270 265 LSAGIEAIQRNREDMTAQSG AmpR LSAGIEAIQRNREDMTAQSG t c a g t g a g g c a c c t a t c t c a g c g a t c t g t c t a t t t c g t t c a t c c a t a g t t g c c t g a c t c c a g t c a c t c c g t g g a t a g a g t c g c t a g a c a g a t a a a g c a a g t a g g t a t c a a c g g a c t g a g g

6240 260 255 250 245 TTYIVVIRSPKGDPGLAAII AmpR TTYIVVIRSPKGDPGLAAII c c g t c g t g t a g a t a a c t a c g a t a c g g g a g g g c t t a c c a t c t g g c c c c a g t g c t g c a a t g a g g c a g c a c a t c t a t t g a t g c t a t g c c c t c c c g a a t g g t a g a c c g g g g t c a c g a c g t t a c t

6300 240 235 230 225 GRSGREGAGSKDAIFWGAPL AmpR GRSGREGAGSKDAIFWGAPL t a c c g c g a g a c c c a c g c t c a c c g g c t c c a g a t t t a t c a g c a a t a a a c c a g c c a g c c g g a a a t g g c g c t c t g g g t g c g a g t g g c c g a g g t c t a a a t a g t c g t t a t t t g g t c g g t c g g c c t t

6360 220 215 210 205 ASRLLPGAVKDAEMWDILQQ AmpR ASRLLPGAVKDAEMWDILQQ g g g c c g a g c g c a g a a g t g g t c c t g c a a c t t t a t c c g c c t c c a t c c a g t c t a t t a a t t g t t c c c g g c t c g c g t c t t c a c c a g g a c g t t g a a a t a g g c g g a g g t a g g t c a g a t a a t t a a c a a

6420 200 195 190 185 RSALTLLEGTLLKRLTTAMA AmpR RSALTLLEGTLLKRLTTAMA g c c g g g a a g c t a g a g t a a g t a g t t c g c c a g t t a a t a g t t t g c g c a a c g t t g t t g c c a t t g c g g c c c t t c g a t c t c a t t c a t c a a g c g g t c a a t t a t c a a a c g c g t t g c a a c a a c g g t a a c

6480 180 175 170 165 VPMTTDREDNPIAENLEPEW AmpR VPMTTDREDNPIAENLEPEW c t a c a g g c a t c g t g g t g t c a c g c t c g t c g t t t g g t a t g g c t t c a t t c a g c t c c g g t t c c c g a t g t c c g t a g c a c c a c a g t g c g a g c a g c a a a c c a t a c c g a a g t a a g t c g a g g c c a a g g g

6540 160 155 150 145 RDLRTVHDGMNHLFATLEKP AmpR RDLRTVHDGMNHLFATLEKP a a c g a t c a a g g c g a g t t a c a t g a t c c c c c a t g t t g t g c a a a a a a g c g g t t a g c t c c t t c g t t g c t a g t t c c g c t c a a t g t a c t a g g g g g t a c a a c a c g t t t t t t c g c c a a t c g a g g a a g c

PvuI 6600 140 135 130 125 GGITTLLLNAATNDSMTIAA AmpR GGITTLLLNAATNDSMTIAA g t c c t c c g a t c g t t g t c a g a a g t a a g t t g g c c g c a g t g t t a t c a c t c a t g g t t a t g g c a g c a g g a g g c t a g c a a c a g t c t t c a t t c a a c c g g c g t c a c a a t a g t g a g t a c c a a t a c c g t c

ScaI 6660 120 115 110 105 SCLERVTMGDTLHKETVPSY AmpR SCLERVTMGDTLHKETVPSY c a c t g c a t a a t t c t c t t a c t g t c a t g c c a t c c g t a a g a t g c t t t t c t g t g a c t g g t g a g t g t g a c g t a t t a a g a g a a t g a c a g t a c g g t a g g c a t t c t a c g a a a a g a c a c t g a c c a c t c a

6720 100 95 90 85 EVLDNQSYHIRRGLQEQGAD AmpR EVLDNQSYHIRRGLQEQGAD a c t c a a c c a a g t c a t t c t g a g a a t a g t g t a t g c g g c g a c c g a g t t g c t c t t g c c c g g c g t t g a g t t g g t t c a g t a a g a c t c t t a t c a c a t a c g c c g c t g g c t c a a c g a g a a c g g g c c g c a

6780 80 75 70 65 IRSLVAGCLLVKFTSMMPFR AmpR A T A A T A C C G C G C C A C A T A G C Amp-R IRSLVAGCLLVKFTSMMPFR c a a t a c g g g a t a a t a c c g c g c c a c a t a g c a g a a c t t t a a a a g t g c t c a t c a t t g g a a a a c g t t a t g c c c t a t t a t g g c g c g g t g t a t c g t c t t g a a a t t t t c a c g a g t a g t a a c c t t t t g

6840 60 55 50 45 EEPRFSELIKGSNLDLEIYG AmpR EEPRFSELIKGSNLDLEIYG g t t c t t c g g g g c g a a a a c t c t c a a g g a t c t t a c c g c t g t t g a g a t c c a g t t c g a t g t a a c c a a g a a g c c c c g c t t t t g a g a g t t c c t a g a a t g g c g a c a a c t c t a g g t c a a g c t a c a t t g

6900 40 35 30 25 VRAGLQDEADKVKVLTEPH A AmpR VRAGLQDEADKVKVLTEPHA c c a c t c g t g c a c c c a a c t g a t c t t c a g c a t c t t t t a c t t t c a c c a g c g t t t c t g g g t g a g g g t g a g c a c g t g g g t t g a c t a g a a g t c g t a g a a a a t g a a a g t g g t c g c a a a g a c c c a c t c

6960 20 15 10 5 signal sequence FVPLCFAAFFPILAVRFHQI AmpR FVPLCFAAFFPILAVRFHQI c a a a a a c a g g a a g g c a a a a t g c c g c a a a a a a g g g a a t a a g g g c g a c a c g g a a a t g t t g a a g t t t t t g t c c t t c c g t t t t a c g g c g t t t t t t c c c t t a t t c c c g c t g t g c c t t t a c a a c t t

SspI 7020 1 signal sequ... SM AmpR AmpR promoter SM t a c t c a t a c t c t t c c t t t t t c a a t a t t a t t g a a g c a t t t a t c a g g g t t a t t g t c t c a t g a a t g a g t a t g a g a a g g a a a a a g t t a t a a t a a c t t c g t a a a t a g t c c c a a t a a c a g a g t a c t

7080 AmpR promoter g c g g a t a c a t a t t t g a a t g t a t t t a g a a a a a t a a a c a a a t a g g g g t t c c g c g c a c a t t t c c g c c t a t g t a t a a a c t t a c a t a a a t c t t t t t a t t t g t t t a t c c c c a a g g c g c g t g t a a a g

SgrDI 7140 c c c g a a a a g t g c c a c c t g a c g t c g a c g g a t c g g g a g a t c t c c c g a t c c c c t a t g g t g c a c g g g c t t t t c a c g g t g g a c t g c a g c t g c c t a g c c c t c t a g a g g g c t a g g g g a t a c c a c g t g

7200 A T G T T A G A C G A G A C T A C G G C pRS-marker t c t c a g t a c a a t c t g c t c t g a t g c c g c a t a g t t a a g c c a g t a t c t g c t c c c t g c t t g t g t a g a g t c a t g t t a g a c g a g a c t a c g g c g t a t c a a t t c g g t c a t a g a c g a g g g a c g a a c a c a

7260 g t t g g a g g t c g c t g a g t a g t g c g c g a g c a a a a t t t a a g c t a c a a c a a g g c a a g g c t t g a c c a a c c t c c a g c g a c t c a t c a c g c g c t c g t t t t a a a t t c g a t g t t g t t c c g t t c c g a a c t g

7320 c g a c a a t t g c a t g a a g a a t c t g c t t a g g g t t a g g c g t t t t g c g c t g c t t c g c g a t g t a c g g c t g t t a a c g t a c t t c t t a g a c g a a t c c c a a t c c g c a a a a c g c g a c g a a g c g c t a c a t g c

SpeIMluI 7380 CMV enhancer g g c c a g a t a t a c g c g t t g a c a t t g a t t a t t g a c t a g t t a t t a a t a g t a a t c a a t t a c g g g c c g g t c t a t a t g c g c a a c t g t a a c t a a t a a c t g a t c a a t a a t t a t c a t t a g t t a a t g c c c

7440 CMV enhancer g t c a t t a g t t c a t a g c c c a t a t a t g g a g t t c c g c g t t a c a t a a c t t a c g g t a a a t g g c c c c a g t a a t c a a g t a t c g g g t a t a t a c c t c a a g g c g c a a t g t a t t g a a t g c c a t t t a c c g g g

7500 CMV enhancer g c c t g g c t g a c c g c c c a a c g a c c c c c g c c c a t t g a c g t c a a t a a t g a c g t a t g t t c c c a t c g g a c c g a c t g g c g g g t t g c t g g g g g c g g g t a a c t g c a g t t a t t a c t g c a t a c a a g g g t a

7560 CMV enhancer a g t a a c g c c a a t a g g g a c t t t c c a t t g a c g t c a a t g g g t g g a g t a t t t a c g g t a a a c t g c t c a t t g c g g t t a t c c c t g a a a g g t a a c t g c a g t t a c c c a c c t c a t a a a t g c c a t t t g a c g

NdeI 7620 CMV enhancer c c a c t t g g c a g t a c a t c a a g t g t a t c a t a t g c c a a g t a c g c c c c c t a t t g a c g t c a a t g a g g t g a a c c g t c a t g t a g t t c a c a t a g t a t a c g g t t c a t g c g g g g g a t a a c t g c a g t t a c t

7680 CMV enhancer c g g t a a a t g g c c c g c c t g g c a t t a t g c c c a g t a c a t g a c c t t a t g g g a c t t t c c t a c t t g g c c a t t t a c c g g g c g g a c c g t a a t a c g g g t c a t g t a c t g g a a t a c c c t g a a a g g a t g a a c

SnaBI 7740 CMV enhancer CMV promoter g c a g t a c a t c t a c g t a t t a g t c a t c g c t a t t a c c a t g g t g a t g c g g t t t t g g c a g t a c a t c g t c a t g t a g a t g c a t a a t c a g t a g c g a t a a t g g t a c c a c t a c g c c a a a a c c g t c a t g t a

7800 CMV promoter c a a t g g g c g t g g a t a g c g g t t t g a c t c a c g g g g a t t t c c a a g t c t c c a c c c c a t t g a c g t g t t a c c c g c a c c t a t c g c c a a a c t g a g t g c c c c t a a a g g t t c a g a g g t g g g g t a a c t g c a

7860 CMV promoter c a a t g g g a g t t t g t t t t g g c a c c a a a a t c a a c g g g a c t t t c c a a a a t g t c g t a a c a a c t c g t t a c c c t c a a a c a a a a c c g t g g t t t t a g t t g c c c t g a a a g g t t t t a c a g c a t t g t t g a g

7920 CMV promoter c g c c c c a t t g a c g c a a a t g g g c g g t a g g c g t g t a c g g t g g g a g g t c t a t a t a a g c a g c g c g c g g g g t a a c t g c g t t t a c c c g c c a t c c g c a c a t g c c a c c c t c c a g a t a t a t t c g t c g c g

7980 5' LTR (truncated) g t t t t g c c t g t a c t g g g t c t c t c t g g t t a g a c c a g a t c t g a g c c t g g g a g c t c t c t g g c t c a a a a c g g a c a t g a c c c a g a g a g a c c a a t c t g g t c t a g a c t c g g a c c c t c g a g a g a c c g a

8040 5' LTR (truncated) a a c t a g g g a a c c c a c t g c t t a a g c c t c a a t a a a g c t t g c c t t g a g t g c t t c a a g t a g t g t t t g a t c c c t t g g g t g a c g a a t t c g g a g t t a t t t c g a a c g g a a c t c a c g a a g t t c a t c a c a

8100 5' LTR (truncated) g t g c c c g t c t g t t g t g t g a c t c t g g t a a c t a g a g a t c c c t c a g a c c c t t t t a g t c a g t g t c a c g g g c a g a c a a c a c a c t g a g a c c a t t g a t c t c t a g g g a g t c t g g g a a a a t c a g t c a c a

8160 5' LTR (truncated) g g a a a a t c t c t a g c a g t g g c g c c c g a a c a g g g a c t t g a a a g c g a a a g g g a a a c c a g a g g a c c t t t t a g a g a t c g t c a c c g c g g g c t t g t c c c t g a a c t t t c g c t t t c c c t t t g g t c t c c t

8220 HIV-1 Ψ g c t c t c t c g a c g c a g g a c t c g g c t t g c t g a a g c g c g c a c g g c a a g a g g c g a g g g g c g g c g c g a g a g a g c t g c g t c c t g a g c c g a a c g a c t t c g c g c g t g c c g t t c t c c g c t c c c c g c c g c

8280 HIV-1 Ψ a c t g g t g a g t a c g c c a a a a a t t t t g a c t a g c g g a g g c t a g a a g g a g a g a g a t g g g t g c g a t g a c c a c t c a t g c g g t t t t t a a a a c t g a t c g c c t c c g a t c t t c c t c t c t c t a c c c a c g c t

8340 HIV-1 Ψ g a g c g t c a g t a t t a a g c g g g g g a g a a t t a g a t c g c g a t g g g a a a a a a t t c g g t t a a g g c c c t c g c a g t c a t a a t t c g c c c c c t c t t a a t c t a g c g c t a c c c t t t t t t a a g c c a a t t c c g g

8400 a g g g g g a a a g a a a a a a t a t a a a t t a a a a c a t a t a g t a t g g g c a a g c a g g g a g c t a g a a c g t c c c c c t t t c t t t t t t a t a t t t a a t t t t g t a t a t c a t a c c c g t t c g t c c c t c g a t c t t g c

8460 a t t c g c a g t t a a t c c t g g c c t g t t a g a a a c a t c a g a a g g c t g t a g a c a a a t a c t g g g a c a t a a g c g t c a a t t a g g a c c g g a c a a t c t t t g t a g t c t t c c g a c a t c t g t t t a t g a c c c t g t

8520 g c t a c a a c c a t c c c t t c a g a c a g g a t c a g a a g a a c t t a g a t c a t t a t a t a a t a c a g t a g c c g a t g t t g g t a g g g a a g t c t g t c c t a g t c t t c t t g a a t c t a g t a a t a t a t t a t g t c a t c g

8580 a a c c c t c t a t t g t g t g c a t c a a a g g a t a g a g a t a a a a g a c a c c a a g g a a g c t t t a g a c a a t t g g g a g a t a a c a c a c g t a g t t t c c t a t c t c t a t t t t c t g t g g t t c c t t c g a a a t c t g t t

NotI 8640 g a t a g a g g a a g a g c a a a a c a a a a g t a a g a c c a c c g c a c a g c a a g c g g c c g c t g a t c t t c a c t a t c t c c t t c t c g t t t t g t t t t c a t t c t g g t g g c g t g t c g t t c g c c g g c g a c t a g a a g t

8700 MRDNWRSELYKYKV g a c c t g g a g g a g g a g a t a t g a g g g a c a a t t g g a g a a g t g a a t t a t a t a a a t a t a a a g t a g c t g g a c c t c c t c c t c t a t a c t c c c t g t t a a c c t c t t c a c t t a a t a t a t t t a t a t t t c a t c

8760 VKIEPLGVAPTKAKRRVVQR t a a a a a t t g a a c c a t t a g g a g t a g c a c c c a c c a a g g c a a a g a g a a g a g t g g t g c a g a g a g a t t t t t a a c t t g g t a a t c c t c a t c g t g g g t g g t t c c g t t t c t c t t c t c a c c a c g t c t c t c

8820 RRE EKRAVGIGALFLGFLGAAGS a a a a a a g a g c a g t g g g a a t a g g a g c t t t g t t c c t t g g g t t c t t g g g a g c a g c a g g a a g c a t t t t t t c t c g t c a c c c t t a t c c t c g a a a c a a g g a a c c c a a g a a c c c t c g t c g t c c t t c g t

8880 RRE TMGAASMTLTVQARQLLSGI c t a t g g g c g c a g c g t c a a t g a c g c t g a c g g t a c a g g c c a g a c a a t t a t t g t c t g g t a t a g g a t a c c c g c g t c g c a g t t a c t g c g a c t g c c a t g t c c g g t c t g t t a a t a a c a g a c c a t a t c

8940 RRE VQQQNNLLRAIEAQQHLLQL t g c a g c a g c a g a a c a a t t t g c t g a g g g c t a t t g a g g c g c a a c a g c a t c t g t t g c a a c t c a a c g t c g t c g t c t t g t t a a a c g a c t c c c g a t a a c t c c g c g t t g t c g t a g a c a a c g t t g a g t

9000 RRE TVWGIKQLQARILAVERYLK c a g t c t g g g g c a t c a a g c a g c t c c a g g c a a g a a t c c t g g c t g t g g a a a g a t a c c t a a a g g g t c a g a c c c c g t a g t t c g t c g a g g t c c g t t c t t a g g a c c g a c a c c t t t c t a t g g a t t t c c

AleI 9060 RRE DQQLLGIWGCSGKLICTTAV a t c a a c a g c t c c t g g g g a t t t g g g g t t g c t c t g g a a a a c t c a t t t g c a c c a c t g c t g t g c t a g t t g t c g a g g a c c c c t a a a c c c c a a c g a g a c c t t t t g a g t a a a c g t g g t g a c g a c a c g

9120 PWNASWSNKSLEQIWNHTTW c t t g g a a t g c t a g t t g g a g t a a t a a a t c t c t g g a a c a g a t t t g g a a t c a c a c g a c c t g g a g a a c c t t a c g a t c a a c c t c a t t a t t t a g a g a c c t t g t c t a a a c c t t a g t g t g c t g g a c c t

9180 MEWDREINNYTSLIHSLIEE t g g a g t g g g a c a g a g a a a t t a a c a a t t a c a c a a g c t t a a t a c a c t c c t t a a t t g a a g a a t a c c t c a c c c t g t c t c t t t a a t t g t t a a t g t g t t c g a a t t a t g t g a g g a a t t a a c t t c t t a

9240 1 5 10 KNEQELLELDKWAS gp41 peptide SQNQQEKNEQELLELDKWAS c g c a a a a c c a g c a a g a a a a g a a t g a a c a a g a a t t a t t g g a a t t a g a t a a a t g g g c a a g t t g c g t t t t g g t c g t t c t t t t c t t a c t t g t t c t t a a t a a c c t t a a t c t a t t t a c c c g t t c a a

9300 15 L (in frame with gp41 peptide) WNWFNITNWLWYIKLFIMI gp41 peptide LWNWFNITNWLWYIKLFIMI t g t g g a a t t g g t t t a a c a t a a c a a a t t g g c t g t g g t a t a t a a a a t t a t t c a t a a t g a t a g a c a c c t t a a c c a a a t t g t a t t g t t t a a c c g a c a c c a t a t a t t t t a a t a a g t a t t a c t a t c

9360 (in frame with gp41 peptide) VGGLVGLRIVFAVLSIVNRV VGGLVGLRIVFAVLSIVNRV t a g g a g g c t t g g t a g g t t t a a g a a t a g t t t t t g c t g t a c t t t c t a t a g t g a a t a g a g t t a a t c c t c c g a a c c a t c c a a a t t c t t a t c a a a a a c g a c a t g a a a g a t a t c a c t t a t c t c a a t

9420 (in frame with gp41 peptide) RQGYSPLSFQTHLPTPRGPD RQGYSPLSFQTHLPTPRGPD g g c a g g g a t a t t c a c c a t t a t c g t t t c a g a c c c a c c t c c c a a c c c c g a g g g g a c c c g a c a c c g t c c c t a t a a g t g g t a a t a g c a a a g t c t g g g t g g a g g g t t g g g g c t c c c c t g g g c t g t

9480 (in frame with gp41 peptide) RPEGIEEEGGERDRDRSIRL RPEGIEEEGGERDRDRSIRL g g c c c g a a g g a a t a g a a g a a g a a g g t g g a g a g a g a g a c a g a g a c a g a t c c a t t c g a t t a g c c g g g c t t c c t t a t c t t c t t c t t c c a c c t c t c t c t c t g t c t c t g t c t a g g t a a g c t a a t c

9540 (in frame with gp41 peptide) VNGSALRAPILQTNGSIHPQ cPPT/CTS VNGSALRAPILQTNGSIHPQ t g a a c g g a t c g g c a c t g c g t g c g c c a a t t c t g c a g a c a a a t g g c a g t a t t c a t c c a c a a t a c t t g c c t a g c c g t g a c g c a c g c g g t t a a g a c g t c t g t t t a c c g t c a t a a g t a g g t g t t a

9600 F* cPPT/CTS F* t t t a a a a g a a a a g g g g g g a t t g g g g g g t a c a g t g c a g g g g a a a g a a t a g t a g a c a t a a t a a a a t t t t c t t t t c c c c c c t a a c c c c c c a t g t c a c g t c c c c t t t c t t a t c a t c t g t a t t a t

9660 cPPT/CTS g c a a c a g a c a t a c a a a c t a a a g a a t t a c a a a a a c a a a t t a c a a a a a t t c a a a a t t t t c g g c g t t g t c t g t a t g t t t g a t t t c t t a a t g t t t t t g t t t a a t g t t t t t a a g t t t t a a a a g c c

9720 tetO tetracycline response element g t t t a t t a c a g g g a c a g c a g a g a t c c a g t t t g g t t a g a t c t c g a g t t t a c c a c t c c c t a t c a a a t a a t g t c c c t g t c g t c t c t a g g t c a a a c c a a t c t a g a g c t c a a a t g g t g a g g g a t a

9780 tetO tetO tetracycline response element c a g t g a t a g a g a a a a g t g a a a g t c g a g t t t a c c a c t c c c t a t c a g t g a t a g a g a a a a g t g g t c a c t a t c t c t t t t c a c t t t c a g c t c a a a t g g t g a g g g a t a g t c a c t a t c t c t t t t c a c

9840 tetO tetracycline response element a a a g t c g a g t t t a c c a c t c c c t a t c a g t g a t a g a g a a a a g t g a a a g t c g a g t t t a c c a c t t t t c a g c t c a a a t g g t g a g g g a t a g t c a c t a t c t c t t t t c a c t t t c a g c t c a a a t g g t g a

9900 tetO tetO tetracycline response element c c c t a t c a g t g a t a g a g a a a a g t g a a a g t c g a g t t t a c c a c t c c c t a t c a g t g a t a g a g a g g g a t a g t c a c t a t c t c t t t t c a c t t t c a g c t c a a a t g g t g a g g g a t a g t c a c t a t c t c t

9960 tetO tetracycline response element a a a g t g a a a g t c g a g t t t a c c a c t c c c t a t c a g t g a t a g a g a a a a g t g a a a g t c g a g t t t t t t c a c t t t c a g c t c a a a t g g t g a g g g a t a g t c a c t a t c t c t t t t c a c t t t c a g c t c a a a

10,020 tetO tetracycline response element minimal CMV promoter a c c a c t c c c t a t c a g t g a t a g a g a a a a g t g a a a g t c g a g c t c g g t a c c c g g g t c g a g g t a t g g t g a g g g a t a g t c a c t a t c t c t t t t c a c t t t c a g c t c g a g c c a t g g g c c c a g c t c c a t

10,080 minimal CMV promoter A G C T C G T T T A G T G A A C C G T C A G A T C LNCX g g c g t g t a c g g t g g g a g g c c t a t a t a a g c a g a g c t c g t t t a g t g a a c c g t c a g a t c g c c t c c g c a c a t g c c a c c c t c c g g a t a t a t t c g t c t c g a g c a a a t c a c t t g g c a g t c t a g c g g a

10,140 g g a g a c g c c a t c c a c g c t g t t t t g a c c t c c a t a g a a g a c a c c g g g a c c g a t c c a g c c t c c c c t c t g c g g t a g g t g c g a c a a a a c t g g a g g t a t c t t c t g t g g c c c t g g c t a g g t c g g a g g

10,148 g c g g c c c c c g c c g g g g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.