Addgene AAV-KrasG13A/sgKras/Cre - Sequence Analyzer Skip to main content
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Sequence Analyzer: AAV-KrasG13A/sgKras/Cre


Map View

EcoNI (7852) HindIII (7798) M13 rev M13 Reverse (7769 .. 7785) lac operator M13/pUC Reverse (7750 .. 7772) CAP binding site BspQI - SapI (7556) pBR322ori-F (7284 .. 7303) BsaI (6485) NmeAIII (6404) Amp-R (5982 .. 6001) AmpR promoter AatII (5632) ZraI (5630) pBRforEco (5573 .. 5591) pGEX 3' (5513 .. 5535) pRS-marker (5394 .. 5413) F1ori-F (5219 .. 5240) NaeI (5049) NgoMIV (5047) F1ori-R (5009 .. 5028) M13/pUC Forward (4703 .. 4725) M13 Forward (4694 .. 4711) KpnI (4680) Acc65I (4676) XbaI (4661) PshAI (3994) NsiI (195) NcoI (211) Kozak sequence HpaI (360) BstZ17I (367) AvrII (1143) AleI (1145) BsiWI (1378) BstXI (2097) PflMI (2115) XcmI (2119) PaeR7I - PspXI - XhoI (2231) hU6-F (2241 .. 2261) U6 promoter LKO.1 5' (2412 .. 2431) gRNA scaffold AscI (2597) MSCV-rev (2673 .. 2696) mPGK-F (3070 .. 3089) KflI (3129) SV40 NLS RsrII (3460) BstBI (3568) CsiI - SexAI * (3586) AsiSI (3617) PaqCI (3768) AAV-KrasG13A/sgKras/Cre 7974 bp

Sequence View

5ʹ 3ʹ 60 t t g g c c a c t c c c t c t c t g c g c g c t c g c t c g c t c a c t g a g g c c g g g c g a c c a a a g g t c g c c a a c c g g t g a g g g a g a g a c g c g c g a g c g a g c g a g t g a c t c c g g c c c g c t g g t t t c c a g c g g

120 c g a c g c c c g g g c t t t g c c c g g g c g g c c t c a g t g a g c g a g c g a g c g c g c a g a g a g g g a g t g g c t g c g g g c c c g a a a c g g g c c c g c c g g a g t c a c t c g c t c g c t c g c g c g t c t c t c c c t c a c

180 g c c a a c t c c a t c a c t a g g g g t t c c t g g a g g g g t g g a g t c g t g a c g t a a a g a t c t g a t a t c c g g t t g a g g t a g t g a t c c c c a a g g a c c t c c c c a c c t c a g c a c t g c a t t t c t a g a c t a t a g

NcoINsiI 240 Kozak sequence a t c g a t c g c g a t g c a t t a a t t a a g c g g c c g c c a t g g c a g t t c t t t t g t a t c c a t t t g t c t t a g c t a g c g c t a c g t a a t t a a t t c g c c g g c g g t a c c g t c a a g a a a a c a t a g g t a a a c a g a

300 c t t t a t c t g c a a t a a t a t a t g t t c t a c a a g g g a a a g g a g a t a a a g t g t a t a t a t t a a t t t g a a a t a g a c g t t a t t a t a t a c a a g a t g t t c c c t t t c c t c t a t t t c a c a t a t a t a a t t a a a

HpaI 360 t a t t t c a a a a a t g t t t g g a a a a g c a g a c t a g g a a a c a a c a g t g t t g t a t t c a g g g c t g t t a t a a a g t t t t t a c a a a c c t t t t c g t c t g a t c c t t t g t t g t c a c a a c a t a a g t c c c g a c a a

BstZ17I 420 a a c t g t a t a c a g t g c c a g c a c t t a g t g c a c c t g t t g g g a g t g c a g t g t a t t a t a a g c a a g t t g a c a t a t g t c a c g g t c g t g a a t c a c g t g g a c a a c c c t c a c g t c a c a t a a t a t t c g t t c

480 g c a g a a g t c a c a g a g g t t a c t g a g a a a c t c t t t t a t g t t c t t t a c a t t t t t t t t c t t g c c c g t c t t c a g t g t c t c c a a t g a c t c t t t g a g a a a a t a c a a g a a a t g t a a a a a a a a g a a c g g

540 a a a a a t a g t c t t t a t c t c t g a a t t t c a t t c a a t t t t a t t t c c t t g t a c a g a g g t a c t g t t t t t t t a t c a g a a a t a g a g a c t t a a a g t a a g t t a a a a t a a a g g a a c a t g t c t c c a t g a c a a

600 t t t a g t a c t c a a a g g a t t a t a t g t t a a a a t t g a t c t g a t c c t a t a c c a a a t a t t t a a a c c a a a t c a t g a g t t t c c t a a t a t a c a a t t t t a a c t a g a c t a g g a t a t g g t t t a t a a a t t t g g

660 c a t t t c c c a c a g a t a g t t t a t a g a t t a a a a t g c c a t c a a a t a t t a t c t t t a t t t g c t t c a g t a a a g g g t g t c t a t c a a a t a t c t a a t t t t a c g g t a g t t t a t a a t a g a a a t a a a c g a a g t

720 t t g c c t a a a t a t g t c t t t c c c c a g c a c a g t g c a g t t t t g a c a c c a g c t t c g g c t t c c t a t a a c g g a t t t a t a c a g a a a g g g g t c g t g t c a c g t c a a a a c t g t g g t c g a a g c c g a a g g a t a

780 t t t g t t g c c c t t t a t t g c a g a a c t g c t c t g a t g g c t t t a a a a c a g t t c t c t g c a g t t g t t a a a c a a c g g g a a a t a a c g t c t t g a c g a g a c t a c c g a a a t t t t g t c a a g a g a c g t c a a c a a

840 g g c t c c a a c a c a g a t g t t c t t a g g c t a c c t a a c t t c t a a c t t t t a a t a t c c a g t c a a c a a c c g a g g t t g t g t c t a c a a g a a t c c g a t g g a t t g a a g a t t g a a a a t t a t a g g t c a g t t g t t

900 a g a a t a c c g c a a g g g t a g g t g t t g g g a t a g c t g t c g a c a a g c t c a t g c g g g t g t g t c c a c t c t t a t g g c g t t c c c a t c c a c a a c c c t a t c g a c a g c t g t t c g a g t a c g c c c a c a c a g g t g

960 a g g g t a t a g c g t a c t a t g c a g a a t a t t t g t a c t g a g t g a a g t c a t g a t a c a t t c c t t t g a t c c c a t a t c g c a t g a t a c g t c t t a t a a a c a t g a c t c a c t t c a g t a c t a t g t a a g g a a a c t

1020 g a g c c a t t a g c t g c t a c a a a a c a g t a a t c t g g c t g t t t a g a t c a a c a a g c t a a a t g a t a g c t c g g t a a t c g a c g a t g t t t t g t c a t t a g a c c g a c a a a t c t a g t t g t t c g a t t t a c t a t c

1080 a a g a t g a a a g t a c t g g t t t c c a t g t a t t t t t a t t a a g t g t t g a t g a g a a a g t t g t a a g t g t t c t a c t t t c a t g a c c a a a g g t a c a t a a a a a t a a t t c a c a a c t a c t c t t t c a a c a t t c a c

1140 a c t t a c a g g t t a c t c t g t a c a t c t g t a g t c a c t g a a t t c g g a a t a t c t t a g a g t t t t a c a t g a a t g t c c a a t g a g a c a t g t a g a c a t c a g t g a c t t a a g c c t t a t a g a a t c t c a a a a t g t

AvrIIAleI 1200 c a c c t a g g t g a g t g t t a a a a t a t t g a t a a a g t t t t t g a t a a t c t t g t g t g a g a c a t g t t c g t g g a t c c a c t c a c a a t t t t a t a a c t a t t t c a a a a a c t a t t a g a a c a c a c t c t g t a c a a g

1260 t a a t t t a g t t g t a t t t t a t t a t t t t t a t t g t a a g g c c t g c t g a a a a t g a c t g a g t a t a a a a t t a a a t c a a c a t a a a a t a a t a a a a a t a a c a t t c c g g a c g a c t t t t a c t g a c t c a t a t t t

1320 c t a g t a g t c g t t g g a g c t g g t g c c g t g g g g a a a t c a g c g c t a a c g a t a c a g c t a a t t c a g g a t c a t c a g c a a c c t c g a c c a c g g c a c c c c t t t a g t c g c g a t t g c t a t g t c g a t t a a g t c

BsiWI 1380 a a t c a c t t t g t g g a t g a g t a t g a c c c t a c g a t a g a g g t a a c g c t g c t c t a c a g t c t g c g t t t a g t g a a a c a c c t a c t c a t a c t g g g a t g c t a t c t c c a t t g c g a c g a g a t g t c a g a c g c a

1440 a c g c t t g t a a a g g a c g g c a g c c a g c c g c t t t g a a a a a g a t a t c a t t t t t a t a t t t a t t a g t g c g a a c a t t t c c t g c c g t c g g t c g g c g a a a c t t t t t c t a t a g t a a a a a t a t a a a t a a t c

1500 a a a a t t a t a t t g a a a g t t a t t t c a g t t a t a t g t g a t g t c c t t t a g t t c c a a g g c t t t a a a t t t t a a t a t a a c t t t c a a t a a a g t c a a t a t a c a c t a c a g g a a a t c a a g g t t c c g a a a t t t

1560 c t g g g t g t t a g g g a a c c a t a g g t g c a a g a a a g t c c a c t t c t c a t g a g a g c t c a c c a c a g a g a c c c a c a a t c c c t t g g t a t c c a c g t t c t t t c a g g t g a a g a g t a c t c t c g a g t g g t g t c t

1620 g a a a g a a a g t c c a c t t c t c a t g a g a g c t t a c c a c a g a g a g t a a g g t c c a a g a t g c c a t c c c t t t c t t t c a g g t g a a g a g t a c t c t c g a a t g g t g t c t c t c a t t c c a g g t t c t a c g g t a g g

1680 g t g c t t t g t a t t t g c a t t t t g a t a a a t a a a t t t c a t g a t t t t g t g a g a g t t a a g a a t a t a c a c g a a a c a t a a a c g t a a a a c t a t t t a t t t a a a g t a c t a a a a c a c t c t c a a t t c t t a t a t

1740 g c t t t g c c t t t g a t c t a t t c c t g a a a g g t t t g t t g t t g t t t t a t g g g t t t g t t g t t a t t g c g a a a c g g a a a c t a g a t a a g g a c t t t c c a a a c a a c a a c a a a a t a c c c a a a c a a c a a t a a c

1800 t g t t g t t g t t t g g t g t c t t t t a g g c a g g g t c t t a c t a t g c a g c c c t g a c c g g c c t a g a a c a c a a c a a c a a a c c a c a g a a a a t c c g t c c c a g a a t g a t a c g t c g g g a c t g g c c g g a t c t t g

1860 t c a a c t a t g t a g a c c a g a c t a g c t c t g a a t t c t c a g a g a t c c a t c t a c c t g c c t c t g c t g a g t t g a t a c a t c t g g t c t g a t c g a g a c t t a a g a g t c t c t a g g t a g a t g g a c g g a g a c g a c

1920 c c c a g a t g c t g c g a t t a a a g a t g t a c a c c a c c a t g t a c a g t g a a a g t t a c t c t t t t g a a a g g g t c t a c g a c g c t a a t t t c t a c a t g t g g t g g t a c a t g t c a c t t t c a a t g a g a a a a c t t t

1980 a c a c t t t a t t g t t a t a g a t t a c t t g g a a t g t t g a t g t g a t g a g a a a a t t t g a t t a t a c t a t g t g a a a t a a c a a t a t c t a a t g a a c c t t a c a a c t a c a c t a c t c t t t t a a a c t a a t a t g a t

2040 g g a a a a t a g a a t c a c t t g a t g g t t g a g c t t t t t t t t t a a g a c t t a t t t a t t t t a t g t a a g c c t t t t a t c t t a g t g a a c t a c c a a c t c g a a a a a a a a a t t c t g a a t a a a t a a a a t a c a t t c

BstXI 2100 t g a g t a c a c t g c t t c t c t c t t c a g a c a c a c c a g a a g a g g g c a t c a g a t c c c a t t a a g a t g a c t c a t g t g a c g a a g a g a g a a g t c t g t g t g g t c t t c t c c c g t a g t c t a g g g t a a t t c t a c

PflMIXcmI 2160 g c t g t g a g c c a c c a t g t g g t t g c t g g g a a t t g a a c t c a g g a c c t c t g g a a a g g c a g t c a g c g a c a c t c g g t g g t a c a c c a a c g a c c c t t a a c t t g a g t c c t g g a g a c c t t t c c g t c a g t c

2220 t g c t c t t t t t t t g t t t a a a g a t t t a t t t a t t t t t a t g a a t a c a g t g t c a c t g t c t t c a t a a c g a g a a a a a a a c a a a t t t c t a a a t a a a t a a a a a t a c t t a t g t c a c a g t g a c a g a a g t a t

PaeR7IPspXIXhoI 2280 U6 promoter G A G G G C C T A T T T C C C A T G A T T hU6-F c a c a c a a g a g c t c g a g c t g t g a g g g c c t a t t t c c c a t g a t t c c t t c a t a t t t g c a t a t a c g t g t g t t c t c g a g c t c g a c a c t c c c g g a t a a a g g g t a c t a a g g a a g t a t a a a c g t a t a t g

2340 U6 promoter g a t a c a a g g c t g t t a g a g a g a t a a t t g g a a t t a a t t t g a c t g t a a a c a c a a a g a t a t t a g c t a t g t t c c g a c a a t c t c t c t a t t a a c c t t a a t t a a a c t g a c a t t t g t g t t t c t a t a a t c

2400 U6 promoter t a c a a a a t a c g t g a c g t a g a a a g t a a t a a t t t c t t g g g t a g t t t g c a g t t t t a a a a t t a t a t g t t t t a t g c a c t g c a t c t t t c a t t a t t a a a g a a c c c a t c a a a c g t c a a a a t t t t a a t a

2460 U6 promoter G A C T A T C A T A T G C T T A C C G T LKO.1 5' g t t t t a a a a t g g a c t a t c a t a t g c t t a c c g t a a c t t g a a a g t a t t t c g a t t t c t t g g c t t c a a a a t t t t a c c t g a t a g t a t a c g a a t g g c a t t g a a c t t t c a t a a a g c t a a a g a a c c g a a

2520 U6 promoter gRNA scaffold t a t a t a t c t t g t g g a a a g g a c c a a a c a c c g a c t g a g t a t a a a c t t g t g g g t t t t a g a g c t a t a t a t a g a a c a c c t t t c c t g g t t t g t g g c t g a c t c a t a t t t g a a c a c c c a a a a t c t c g a

2580 gRNA scaffold a g a a a t a g c a a g t t a a a a t a a g g c t a g t c c g t t a t c a a c t t g a a a a a g t g g c a c c g a g t c t c t t t a t c g t t c a a t t t t a t t c c g a t c a g g c a a t a g t t g a a c t t t t t c a c c g t g g c t c a g

AscI 2640 gRNA scaffold PGK promoter g g t g c t t t t t t g t t t g g c g c g c c t a t g g c t t a a t t a a c a t g g c g g c c g c a t t c t a c c g g g c c a c g a a a a a a c a a a c c g c g c g g a t a c c g a a t t a a t t g t a c c g c c g g c g t a a g a t g g c c c

2700 PGK promoter C G T A C G C G A A A T C G T C G G G G C G A C MSCV-rev t a g g g g a g g c g c t t t t c c c a a g g c a g t c t g g a g c a t g c g c t t t a g c a g c c c c g c t g g g c a a t c c c c t c c g c g a a a a g g g t t c c g t c a g a c c t c g t a c g c g a a a t c g t c g g g g c g a c c c g t

2760 PGK promoter c t t g g c g c t a c a c a a g t g g c c t c t g g c c t c g c a c a c a t t c c a c a t c c a c c g g t a g g c g c c g a a c c g c g a t g t g t t c a c c g g a g a c c g g a g c g t g t g t a a g g t g t a g g t g g c c a t c c g c g g

2820 PGK promoter a a c c g g c t c c g t t c t t t g g t g g c c c c t t c g c g c c a c c t t c t a c t c c t c c c c t a g t c a g g a t t g g c c g a g g c a a g a a a c c a c c g g g g a a g c g c g g t g g a a g a t g a g g a g g g g a t c a g t c c t

2880 PGK promoter a g t t c c c c c c c g c c c c g c a g c t c g c g t c g t g c a g g a c g t g a c a a a t g g a a g t a g c a c g t c t c a a g g g g g g g c g g g g c g t c g a g c g c a g c a c g t c c t g c a c t g t t t a c c t t c a t c g t g c a g

2940 PGK promoter t c a c t a g t c t c g t g c a g a t g g a c a g c a c c g c t g a g c a a t g g a a g c g g g t a g g c c t t t g g g a g t g a t c a g a g c a c g t c t a c c t g t c g t g g c g a c t c g t t a c c t t c g c c c a t c c g g a a a c c c

3000 PGK promoter g c a g c g g c c a a t a g c a g c t t t g c t c c t t c g c t t t c t g g g c t c a g a g g c t g g g a a g g g g t g c g t c g c c g g t t a t c g t c g a a a c g a g g a a g c g a a a g a c c c g a g t c t c c g a c c c t t c c c c a c

3060 PGK promoter g g t c c g g g g g c g g g c t c a g g g g c g g g c t c a g g g g c g g g g c g g g c g c c c g a a g g t c c t c c g c c a g g c c c c c g c c c g a g t c c c c g c c c g a g t c c c c g c c c c g c c c g c g g g c t t c c a g g a g g c

3120 PGK promoter C A T T C T G C A C G C T T C A A A A G mPGK-F g a g g c c c g g c a t t c t g c a c g c t t c a a a a g c g c a c g t c t g c c g c g c t g t t c t c c t c t t c c t c t c c g g g c c g t a a g a c g t g c g a a g t t t t c g c g t g c a g a c g g c g c g a c a a g a g g a g a a g g a

KflI 3180 130 PKKKRKV 5 SNLLTV PGK promoter SV40 NLS Cre MPKKKRKVSNLLTV c a t c t c c g g g a c c c g g g a t g c c c a a g a a g a a g a g g a a g g t g t c c a a t t t a c t g a c c g t a c g t a g a g g c c c t g g g c c c t a c g g g t t c t t c t t c t c c t t c c a c a g g t t a a a t g a c t g g c a t g

3240 10 15 20 25 HQNLPALPVDATSDEVRKNL Cre HQNLPALPVDATSDEVRKNL a c c a a a a t t t g c c t g c a t t a c c g g t c g a t g c a a c g a g t g a t g a g g t t c g c a a g a a c c t g a t g g t t t t a a a c g g a c g t a a t g g c c a g c t a c g t t g c t c a c t a c t c c a a g c g t t c t t g g a c t

3300 30 35 40 45 MDMFRDRQAFSEHTWKMLLS Cre MDMFRDRQAFSEHTWKMLLS t g g a c a t g t t c a g g g a t c g c c a g g c g t t t t c t g a g c a t a c c t g g a a a a t g c t t c t g t c c g a c c t g t a c a a g t c c c t a g c g g t c c g c a a a a g a c t c g t a t g g a c c t t t t a c g a a g a c a g g c

3360 50 55 60 65 VCRSWAAWCKLNNRKWFPAE Cre VCRSWAAWCKLNNRKWFPAE t t t g c c g g t c g t g g g c g g c a t g g t g c a a g t t g a a t a a c c g g a a a t g g t t t c c c g c a g a a c a a a c g g c c a g c a c c c g c c g t a c c a c g t t c a a c t t a t t g g c c t t t a c c a a a g g g c g t c t t g

3420 70 75 80 85 PEDVRDYLLYLQARGLAVKT Cre PEDVRDYLLYLQARGLAVKT c t g a a g a t g t t c g c g a t t a t c t t c t a t a t c t t c a g g c g c g c g g t c t g g c a g t a a a a a c t a g a c t t c t a c a a g c g c t a a t a g a a g a t a t a g a a g t c c g c g c g c c a g a c c g t c a t t t t t g a t

RsrII 3480 90 95 100 105 IQQHLGQLNMLHRRSGLPRP Cre IQQHLGQLNMLHRRSGLPRP t c c a g c a a c a t t t g g g c c a g c t a a a c a t g c t t c a t c g t c g g t c c g g g c t g c c a c g a c c a a a g g t c g t t g t a a a c c c g g t c g a t t t g t a c g a a g t a g c a g c c a g g c c c g a c g g t g c t g g t t

3540 110 115 120 125 SDSNAVSLVMRRIRKENVDA Cre SDSNAVSLVMRRIRKENVDA g t g a c a g c a a t g c t g t t t c a c t g g t t a t g c g g c g g a t c c g a a a a g a a a a c g t t g a t g c c g c a c t g t c g t t a c g a c a a a g t g a c c a a t a c g c c g c c t a g g c t t t t c t t t t g c a a c t a c g g c

SexAI*CsiIBstBI 3600 130 135 140 145 GERAKQALAFERTDFDQVRS Cre GERAKQALAFERTDFDQVRS g t g a a c g t g c a a a a c a g g c t c t a g c g t t c g a a c g c a c t g a t t t c g a c c a g g t t c g t t c a c c a c t t g c a c g t t t t g t c c g a g a t c g c a a g c t t g c g t g a c t a a a g c t g g t c c a a g c a a g t g

AsiSI 3660 150 155 160 165 LMENSDRCQDIRNLAFLGIA Cre LMENSDRCQDIRNLAFLGIA t c a t g g a a a a t a g c g a t c g c t g c c a g g a t a t a c g t a a t c t g g c a t t t c t g g g g a t t g c t t a g t a c c t t t t a t c g c t a g c g a c g g t c c t a t a t g c a t t a g a c c g t a a a g a c c c c t a a c g a a

3720 170 175 180 185 YNTLLRIAEIARIRVKDISR Cre YNTLLRIAEIARIRVKDISR a t a a c a c c c t g t t a c g t a t a g c c g a a a t t g c c a g g a t c a g g g t t a a a g a t a t c t c a c g t a t a t t g t g g g a c a a t g c a t a t c g g c t t t a a c g g t c c t a g t c c c a a t t t c t a t a g a g t g c a t

PaqCI 3780 190 195 200 205 TDGGRMLIHIGRTKTLVSTA Cre TDGGRMLIHIGRTKTLVSTA c t g a c g g t g g g a g a a t g t t a a t c c a t a t t g g c a g a a c g a a a a c g c t g g t t a g c a c c g c a g g a c t g c c a c c c t c t t a c a a t t a g g t a t a a c c g t c t t g c t t t t g c g a c c a a t c g t g g c g t c

3840 210 215 220 225 GVEKALSLGVTKLVERWISV Cre GVEKALSLGVTKLVERWISV g t g t a g a g a a g g c a c t t a g c c t g g g g g t a a c t a a a c t g g t c g a g c g a t g g a t t t c c g t c t c a c a t c t c t t c c g t g a a t c g g a c c c c c a t t g a t t t g a c c a g c t c g c t a c c t a a a g g c a g a

3900 230 235 240 245 SGVADDPNNYLFCRVRKNGV Cre SGVADDPNNYLFCRVRKNGV c t g g t g t a g c t g a t g a t c c g a a t a a c t a c c t g t t t t g c c g g g t c a g a a a a a a t g g t g t t g g a c c a c a t c g a c t a c t a g g c t t a t t g a t g g a c a a a a c g g c c c a g t c t t t t t t a c c a c a a c

3960 250 255 260 265 AAPSATSQLSTRALEGIFEA Cre AAPSATSQLSTRALEGIFEA c c g c g c c a t c t g c c a c c a g c c a g c t a t c a a c t c g c g c c c t g g a a g g g a t t t t t g a a g c a a g g c g c g g t a g a c g g t g g t c g g t c g a t a g t t g a g c g c g g g a c c t t c c c t a a a a a c t t c g t t

PshAI 4020 270 275 280 285 THRLIYGAKDDSGQRYLAWS Cre THRLIYGAKDDSGQRYLAWS c t c a t c g a t t g a t t t a c g g c g c t a a g g a t g a c t c t g g t c a g a g a t a c c t g g c c t g g t c t g g a g t a g c t a a c t a a a t g c c g c g a t t c c t a c t g a g a c c a g t c t c t a t g g a c c g g a c c a g a c

4080 290 295 300 305 GHSARVGAARDMARAGVSIP Cre GHSARVGAARDMARAGVSIP g a c a c a g t g c c c g t g t c g g a g c c g c g c g a g a t a t g g c c c g c g c t g g a g t t t c a a t a c c g g c t g t g t c a c g g g c a c a g c c t c g g c g c g c t c t a t a c c g g g c g c g a c c t c a a a g t t a t g g c c

4140 310 315 320 325 EIMQAGGWTNVNIVMNYIRN Cre EIMQAGGWTNVNIVMNYIRN a g a t c a t g c a a g c t g g t g g c t g g a c c a a t g t a a a t a t t g t c a t g a a c t a t a t c c g t a a c c t c t a g t a c g t t c g a c c a c c g a c c t g g t t a c a t t t a t a a c a g t a c t t g a t a t a g g c a t t g g

4200 330 335 340 LDSETGAMVRLLEDGD* Cre LDSETGAMVRLLEDGD* t g g a t a g t g a a a c a g g g g c a a t g g t g c g c c t g c t g g a a g a t g g c g a t t a g c c a t g t t t a a a c c t a t c a c t t t g t c c c c g t t a c c a c g c g g a c g a c c t t c t a c c g c t a a t c g g t a c a a a t t

4260 g g g t t c c t c t a g t g t a c a c g c g t t g t g c c t t c t a g t t g c c a g c c a t c t g t t g t t t g c c c c c c c a a g g a g a t c a c a t g t g c g c a a c a c g g a a g a t c a a c g g t c g g t a g a c a a c a a a c g g g g

4320 t c c c c c g t g c c t t c c t t g a c c c t g g a a g g t g c c a c t c c c a c t g t c c t t t c c t a a t a a a a t a g g g g g c a c g g a a g g a a c t g g g a c c t t c c a c g g t g a g g g t g a c a g g a a a g g a t t a t t t t a

4380 g a g g a a a t t g c a t c g c a t t g t c t g a g t a g g t g t c a t t c t a t t c t g g g g g g t g g g g t g g g g c t c c t t t a a c g t a g c g t a a c a g a c t c a t c c a c a g t a a g a t a a g a c c c c c c a c c c c a c c c c

4440 c a g g a c a g c a a g g g g g a g g a t t g g g a a g a c a a t a g c a g g c a t g c t g g g g a a c g c g t g a t a g t c c t g t c g t t c c c c c t c c t a a c c c t t c t g t t a t c g t c c g t a c g a c c c c t t g c g c a c t a t

4500 t c a g a t c t g t t a c g t a g a t a a g t a g c a t g g c g g g t t a a t c a t t a a c t a c a a g g a a c c c c t a g t c t a g a c a a t g c a t c t a t t c a t c g t a c c g c c c a a t t a g t a a t t g a t g t t c c t t g g g g a

4560 a g t g a t g g a g t t g g c c a c t c c c t c t c t g c g c g c t c g c t c g c t c a c t g a g g c c g c c c g g g c t c a c t a c c t c a a c c g g t g a g g g a g a g a c g c g c g a g c g a g c g a g t g a c t c c g g c g g g c c c g

4620 a a a g c c c g g g c g t c g g g c g a c c t t t g g t c g c c c g g c c t c a g t g a g c g a g c g a g c g c g c a g t t t c g g g c c c g c a g c c c g c t g g a a a c c a g c g g g c c g g a g t c a c t c g c t c g c t c g c g c g t c

Acc65IKpnIXbaI 4680 a g a g g g a g t g g c c a a a g c g c g c a g c t g c c t g c a g g t c g a c t c t a g a g g a t c c c c g g g t a c t c t c c c t c a c c g g t t t c g c g c g t c g a c g g a c g t c c a g c t g a g a t c t c c t a g g g g c c c a t g

4740 M13 fwd T G A C C G G C A G C A A A A T G T M13 Forward G C A A A A T G T T G C A G C A C T G A C C C M13/pUC Forward c g a g c t c g a a t t c a c t g g c c g t c g t t t t a c a a c g t c g t g a c t g g g a a a a c c c t g g c g t t a g c t c g a g c t t a a g t g a c c g g c a g c a a a a t g t t g c a g c a c t g a c c c t t t t g g g a c c g c a a t

4800 c c c a a c t t a a t c g c c t t g c a g c a c a t c c c c c t t t c g c c a g c t g g c g t a a t a g c g a a g a g g g g g t t g a a t t a g c g g a a c g t c g t g t a g g g g g a a a g c g g t c g a c c g c a t t a t c g c t t c t c c

4860 c c c g c a c c g a t c g c c c t t c c c a a c a g t t g c g c a g c c t g a a t g g c g a a t g g c g c c t g a t g c g g g c g t g g c t a g c g g g a a g g g t t g t c a a c g c g t c g g a c t t a c c g c t t a c c g c g g a c t a c g

4920 g g t a t t t t c t c c t t a c g c a t c t g t g c g g t a t t t c a c a c c g c a t a c g t c a a a g c a a c c a t a c c a t a a a a g a g g a a t g c g t a g a c a c g c c a t a a a g t g t g g c g t a t g c a g t t t c g t t g g t a t

4980 f1 ori g t a c g c g c c c t g t a g c g g c g c a t t a a g c g c g g c g g g t g t g g t g g t t a c g c g c a g c g t g a c c a t g c g c g g g a c a t c g c c g c g t a a t t c g c g c c g c c c a c a c c a c c a a t g c g c g t c g c a c t g

5040 f1 ori G A G G A A A G C G A A A G A A G G G A F1ori-R c g c t a c a c t t g c c a g c g c t t a g c g c c c g c t c c t t t c g c t t t c t t c c c t t c c t t t c t c g c c g c g a t g t g a a c g g t c g c g a a t c g c g g g c g a g g a a a g c g a a a g a a g g g a a g g a a a g a g c g g

NgoMIVNaeI 5100 f1 ori a c g t t c g c c g g c t t t c c c c g t c a a g c t c t a a a t c g g g g g c t c c c t t t a g g g t t c c g a t t t t g c a a g c g g c c g a a a g g g g c a g t t c g a g a t t t a g c c c c c g a g g g a a a t c c c a a g g c t a a a

5160 f1 ori a g t g c t t t a c g g c a c c t c g a c c c c a a a a a a c t t g a t t t g g g t g a t g g t t c a c g t a g t g g g t c a c g a a a t g c c g t g g a g c t g g g g t t t t t t g a a c t a a a c c c a c t a c c a a g t g c a t c a c c c

5220 f1 ori G T F1ori-F c c a t c g c c c t g a t a g a c g g t t t t t c g c c c t t t g a c g t t g g a g t c c a c g t t c t t t a a t a g t g g t a g c g g g a c t a t c t g c c a a a a a g c g g g a a a c t g c a a c c t c a g g t g c a a g a a a t t a t c a

5280 f1 ori G G A C T C T T G T T C C A A A C T G G F1ori-F g g a c t c t t g t t c c a a a c t g g a a c a a c a c t c a a c t c t a t c t c g g g c t a t t c t t t t g a t t t a c c t g a g a a c a a g g t t t g a c c t t g t t g t g a g t t g a g a t a g a g c c c g a t a a g a a a a c t a a a t

5340 f1 ori t a a g g g a t t t t g c c g a t t t c g g t c t a t t g g t t a a a a a a t g a g c t g a t t t a a c a a a a a t t t a t t c c c t a a a a c g g c t a a a g c c a g a t a a c c a a t t t t t t a c t c g a c t a a a t t g t t t t t a a a

5400 f1 ori A T G T T A G pRS-marker a a c g c g a a t t t t a a c a a a a t a t t a a c g t t t a c a a t t t t a t g g t g c a c t c t c a g t a c a a t c t t g c g c t t a a a a t t g t t t t a t a a t t g c a a a t g t t a a a a t a c c a c g t g a g a g t c a t g t t a g

5460 A C G A G A C T A C G G C pRS-marker t g c t c t g a t g c c g c a t a g t t a a g c c a g c c c c g a c a c c c g c c a a c a c c c g c t g a c g c g c c c a c g a g a c t a c g g c g t a t c a a t t c g g t c g g g g c t g t g g g c g g t t g t g g g c g a c t g c g c g g g

5520 C C G G G A G C pGEX 3' t g a c g g g c t t g t c t g c t c c c g g c a t c c g c t t a c a g a c a a g c t g t g a c c g t c t c c g g g a g c a c t g c c c g a a c a g a c g a g g g c c g t a g g c g a a t g t c t g t t c g a c a c t g g c a g a g g c c c t c g

5580 T G C A T G T G T C A G A G G pGEX 3' C G G A G C A C pBRforEco t g c a t g t g t c a g a g g t t t t c a c c g t c a t c a c c g a a a c g c g c g a g a c g a a a g g g c c t c g t g a c g t a c a c a g t c t c c a a a a g t g g c a g t a g t g g c t t t g c g c g c t c t g c t t t c c c g g a g c a c

ZraIAatII 5640 T A T G C G G A T A A pBRforEco a t a c g c c t a t t t t t a t a g g t t a a t g t c a t g a t a a t a a t g g t t t c t t a g a c g t c a g g t g g c t a t g c g g a t a a a a a t a t c c a a t t a c a g t a c t a t t a t t a c c a a a g a a t c t g c a g t c c a c c g

5700 AmpR promoter a c t t t t c g g g g a a a t g t g c g c g g a a c c c c t a t t t g t t t a t t t t t c t a a a t a c a t t c a a a t t g a a a a g c c c c t t t a c a c g c g c c t t g g g g a t a a a c a a a t a a a a a g a t t t a t g t a a g t t t a

5760 AmpR promoter a t g t a t c c g c t c a t g a g a c a a t a a c c c t g a t a a a t g c t t c a a t a a t a t t g a a a a a g g a a g t a c a t a g g c g a g t a c t c t g t t a t t g g g a c t a t t t a c g a a g t t a t t a t a a c t t t t t c c t t c

5820 1 5 10 15 signal sequence MSIQHFRVALIPFFAAFCL AmpR promoter AmpR MSIQHFRVALIPFFAAFCL a g t a t g a g t a t t c a a c a t t t c c g t g t c g c c c t t a t t c c c t t t t t t g c g g c a t t t t g c c t t t c a t a c t c a t a a g t t g t a a a g g c a c a g c g g g a a t a a g g g a a a a a a c g c c g t a a a a c g g a a

5880 20 signal sequence PVFA 25 30 35 HPETLVKVKDAEDQLG AmpR PVFAHPETLVKVKDAEDQLG c c t g t t t t t g c t c a c c c a g a a a c g c t g g t g a a a g t a a a a g a t g c t g a a g a t c a g t t g g g t g g a c a a a a a c g a g t g g g t c t t t g c g a c c a c t t t c a t t t t c t a c g a c t t c t a g t c a a c c c a

5940 40 45 50 55 ARVGYIELDLNSGKILESFR AmpR ARVGYIELDLNSGKILESFR g c a c g a g t g g g t t a c a t c g a a c t g g a t c t c a a c a g c g g t a a g a t c c t t g a g a g t t t t c g c c g t g c t c a c c c a a t g t a g c t t g a c c t a g a g t t g t c g c c a t t c t a g g a a c t c t c a a a a g c g

6000 60 65 70 75 PEERFPMMSTFKVLLCGAVL AmpR C G A T A C A C C G C G C C A T A A T Amp-R PEERFPMMSTFKVLLCGAVL c c c g a a g a a c g t t t t c c a a t g a t g a g c a c t t t t a a a g t t c t g c t a t g t g g c g c g g t a t t a g g g c t t c t t g c a a a a g g t t a c t a c t c g t g a a a a t t t c a a g a c g a t a c a c c g c g c c a t a a t

6060 80 85 90 95 SRIDAGQEQLGRRIHYSQND AmpR A Amp-R SRIDAGQEQLGRRIHYSQND t c c c g t a t t g a c g c c g g g c a a g a g c a a c t c g g t c g c c g c a t a c a c t a t t c t c a g a a t g a c a g g g c a t a a c t g c g g c c c g t t c t c g t t g a g c c a g c g g c g t a t g t g a t a a g a g t c t t a c t g

6120 100 105 110 115 LVEYSPVTEKHLTDGMTVRE AmpR LVEYSPVTEKHLTDGMTVRE t t g g t t g a g t a c t c a c c a g t c a c a g a a a a g c a t c t t a c g g a t g g c a t g a c a g t a a g a g a a a a c c a a c t c a t g a g t g g t c a g t g t c t t t t c g t a g a a t g c c t a c c g t a c t g t c a t t c t c t t

6180 120 125 130 135 LCSAAITMSDNTAANLLLTT AmpR LCSAAITMSDNTAANLLLTT t t a t g c a g t g c t g c c a t a a c c a t g a g t g a t a a c a c t g c g g c c a a c t t a c t t c t g a c a a c g a a t a c g t c a c g a c g g t a t t g g t a c t c a c t a t t g t g a c g c c g g t t g a a t g a a g a c t g t t g c

6240 140 145 150 155 IGGPKELTAFLHNMGDHVTR AmpR IGGPKELTAFLHNMGDHVTR a t c g g a g g a c c g a a g g a g c t a a c c g c t t t t t t g c a c a a c a t g g g g g a t c a t g t a a c t c g c t a g c c t c c t g g c t t c c t c g a t t g g c g a a a a a a c g t g t t g t a c c c c c t a g t a c a t t g a g c g

6300 160 165 170 175 LDRWEPELNEAIPNDERDTT AmpR LDRWEPELNEAIPNDERDTT c t t g a t c g t t g g g a a c c g g a g c t g a a t g a a g c c a t a c c a a a c g a c g a g c g t g a c a c c a c g g a a c t a g c a a c c c t t g g c c t c g a c t t a c t t c g g t a t g g t t t g c t g c t c g c a c t g t g g t g c

6360 180 185 190 195 MPVAMATTLRKLLTGELLTL AmpR MPVAMATTLRKLLTGELLTL a t g c c t g t a g c a a t g g c a a c a a c g t t g c g c a a a c t a t t a a c t g g c g a a c t a c t t a c t c t a t a c g g a c a t c g t t a c c g t t g t t g c a a c g c g t t t g a t a a t t g a c c g c t t g a t g a a t g a g a t

NmeAIII 6420 200 205 210 215 ASRQQLIDWMEADKVAGPLL AmpR ASRQQLIDWMEADKVAGPLL g c t t c c c g g c a a c a a t t a a t a g a c t g g a t g g a g g c g g a t a a a g t t g c a g g a c c a c t t c t g c g a a g g g c c g t t g t t a a t t a t c t g a c c t a c c t c c g c c t a t t t c a a c g t c c t g g t g a a g a c

6480 220 225 230 235 RSALPAGWFIADKSGAGERG AmpR RSALPAGWFIADKSGAGERG c g c t c g g c c c t t c c g g c t g g c t g g t t t a t t g c t g a t a a a t c t g g a g c c g g t g a g c g t g g g g c g a g c c g g g a a g g c c g a c c g a c c a a a t a a c g a c t a t t t a g a c c t c g g c c a c t c g c a c c c

BsaI 6540 240 245 250 255 SRGIIAALGPDGKPSRIVVI AmpR SRGIIAALGPDGKPSRIVVI t c t c g c g g t a t c a t t g c a g c a c t g g g g c c a g a t g g t a a g c c c t c c c g t a t c g t a g t t a t c a g a g c g c c a t a g t a a c g t c g t g a c c c c g g t c t a c c a t t c g g g a g g g c a t a g c a t c a a t a g

6600 260 265 270 275 YTTGSQATMDERNRQIAEIG AmpR YTTGSQATMDERNRQIAEIG t a c a c g a c g g g g a g t c a g g c a a c t a t g g a t g a a c g a a a t a g a c a g a t c g c t g a g a t a g g t a t g t g c t g c c c c t c a g t c c g t t g a t a c c t a c t t g c t t t a t c t g t c t a g c g a c t c t a t c c a

6660 280 285 ASLIKHW* AmpR ASLIKHW* g c c t c a c t g a t t a a g c a t t g g t a a c t g t c a g a c c a a g t t t a c t c a t a t a t a c t t t a g a t t c g g a g t g a c t a a t t c g t a a c c a t t g a c a g t c t g g t t c a a a t g a g t a t a t a t g a a a t c t a a

6720 g a t t t a a a a c t t c a t t t t t a a t t t a a a a g g a t c t a g g t g a a g a t c c t t t t t g a t a a t c t c c t a a a t t t t g a a g t a a a a a t t a a a t t t t c c t a g a t c c a c t t c t a g g a a a a a c t a t t a g a g

6780 a t g a c c a a a a t c c c t t a a c g t g a g t t t t c g t t c c a c t g a g c g t c a g a c c c c g t a g a a a a g t a c t g g t t t t a g g g a a t t g c a c t c a a a a g c a a g g t g a c t c g c a g t c t g g g g c a t c t t t t c

6840 ori a t c a a a g g a t c t t c t t g a g a t c c t t t t t t t c t g c g c g t a a t c t g c t g c t t g c a a a c a a a a t a g t t t c c t a g a a g a a c t c t a g g a a a a a a a g a c g c g c a t t a g a c g a c g a a c g t t t g t t t t

6900 ori a a a c c a c c g c t a c c a g c g g t g g t t t g t t t g c c g g a t c a a g a g c t a c c a a c t c t t t t t c c g t t t g g t g g c g a t g g t c g c c a c c a a a c a a a c g g c c t a g t t c t c g a t g g t t g a g a a a a a g g c

6960 ori a a g g t a a c t g g c t t c a g c a g a g c g c a g a t a c c a a a t a c t g t t c t t c t a g t g t a g c c g t a g t t c c a t t g a c c g a a g t c g t c t c g c g t c t a t g g t t t a t g a c a a g a a g a t c a c a t c g g c a t c

7020 ori t t a g g c c a c c a c t t c a a g a a c t c t g t a g c a c c g c c t a c a t a c c t c g c t c t g c t a a t c c t g a a t c c g g t g g t g a a g t t c t t g a g a c a t c g t g g c g g a t g t a t g g a g c g a g a c g a t t a g g a c

7080 ori t t a c c a g t g g c t g c t g c c a g t g g c g a t a a g t c g t g t c t t a c c g g g t t g g a c t c a a g a c g a a a t g g t c a c c g a c g a c g g t c a c c g c t a t t c a g c a c a g a a t g g c c c a a c c t g a g t t c t g c t

7140 ori t a g t t a c c g g a t a a g g c g c a g c g g t c g g g c t g a a c g g g g g g t t c g t g c a c a c a g c c c a g c a t c a a t g g c c t a t t c c g c g t c g c c a g c c c g a c t t g c c c c c c a a g c a c g t g t g t c g g g t c g

7200 ori t t g g a g c g a a c g a c c t a c a c c g a a c t g a g a t a c c t a c a g c g t g a g c t a t g a g a a a g c g c c a a c c t c g c t t g c t g g a t g t g g c t t g a c t c t a t g g a t g t c g c a c t c g a t a c t c t t t c g c g g

7260 ori a c g c t t c c c g a a g g g a g a a a g g c g g a c a g g t a t c c g g t a a g c g g c a g g g t c g g a a c a g g a t g c g a a g g g c t t c c c t c t t t c c g c c t g t c c a t a g g c c a t t c g c c g t c c c a g c c t t g t c c t

7320 ori G G G A A A C G C C T G G T A T C T T T pBR322ori-F g a g c g c a c g a g g g a g c t t c c a g g g g g a a a c g c c t g g t a t c t t t a t a g t c c t g t c g g g t t t c t c g c g t g c t c c c t c g a a g g t c c c c c t t t g c g g a c c a t a g a a a t a t c a g g a c a g c c c a a a

7380 ori c g c c a c c t c t g a c t t g a g c g t c g a t t t t t g t g a t g c t c g t c a g g g g g g c g g a g c c t a t g g g c g g t g g a g a c t g a a c t c g c a g c t a a a a a c a c t a c g a g c a g t c c c c c c g c c t c g g a t a c c

7440 ori a a a a a c g c c a g c a a c g c g g c c t t t t t a c g g t t c c t g g c c t t t t g c t g g c c t t t t g c t c a c t t t t t g c g g t c g t t g c g c c g g a a a a a t g c c a a g g a c c g g a a a a c g a c c g g a a a a c g a g t g

7500 a t g t t c t t t c c t g c g t t a t c c c c t g a t t c t g t g g a t a a c c g t a t t a c c g c c t t t g a g t g a t a c a a g a a a g g a c g c a a t a g g g g a c t a a g a c a c c t a t t g g c a t a a t g g c g g a a a c t c a c t

BspQISapI 7560 g c t g a t a c c g c t c g c c g c a g c c g a a c g a c c g a g c g c a g c g a g t c a g t g a g c g a g g a a g c g c g a c t a t g g c g a g c g g c g t c g g c t t g c t g g c t c g c g t c g c t c a g t c a c t c g c t c c t t c g c

7620 g a a g a g c g c c c a a t a c g c a a a c c g c c t c t c c c c g c g c g t t g g c c g a t t c a t t a a t g c a g c c t t c t c g c g g g t t a t g c g t t t g g c g g a g a g g g g c g c g c a a c c g g c t a a g t a a t t a c g t c g

7680 CAP binding site t g g c a c g a c a g g t t t c c c g a c t g g a a a g c g g g c a g t g a g c g c a a c g c a a t t a a t g t g a g t a c c g t g c t g t c c a a a g g g c t g a c c t t t c g c c c g t c a c t c g c g t t g c g t t a a t t a c a c t c a

7740 -35 -10 CAP binding site lac promoter t a g c t c a c t c a t t a g g c a c c c c a g g c t t t a c a c t t t a t g c t t c c g g c t c g t a t g t t g t g t a t c g a g t g a g t a a t c c g t g g g g t c c g a a a t g t g a a a t a c g a a g g c c g a g c a t a c a a c a c a

HindIII 7800 lac operator M13 rev A G C G G A T A A C A A T T T C A C A C A G G M13/pUC Reverse C A G G A A A C A G C T A T G A C M13 Reverse g g a a t t g t g a g c g g a t a a c a a t t t c a c a c a g g a a a c a g c t a t g a c c a t g a t t a c g c c a a g c c t t a a c a c t c g c c t a t t g t t a a a g t g t g t c c t t t g t c g a t a c t g g t a c t a a t g c g g t t c

EcoNI 7860 c t t g c a t g c c t g c a g g c a g c t g c g c g c t c g a a c t t c a t g c c t g c c g a c c t t c c c c a g g t c g a a c g t a c g g a c g t c c g t c g a c g c g c g a g c t t g a a g t a c g g a c g g c t g g a a g g g g t c c a g

7920 a c g a t c c g g a c g g c g g g t g a g t t c a c a t t t t a r c a g c c g g a c g t g c a r a c t c c g c t g g t g t g c t a g g c c t g c c g c c c a c t c a a g t g t a a a a t y g t c g g c c t g c a c g t y t g a g g c g a c c a c

3ʹ 5ʹ 7974 g t c t a a c g t c g g t t a g g t c c c t t g a a t c a c g g g a c a t a t g t t g g t g t t g g a g g t c a g a t t g c a g c c a a t c c a g g g a a c t t a g t g c c c t g t a t a c a a c c a c a a c c t c c a

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.