Addgene PB-GG-OCT4-1-5-PGK-Puro Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: PB-GG-OCT4-1-5-PGK-Puro Sequencing Result


Map View

gRNA scaffold M13 fwd BstXI (6980) M13 fwd M13 fwd gRNA scaffold M13 fwd gRNA scaffold M13 fwd EcoRI (5506) BstBI (5504) BstZ17I (5230) AccI (5229) MluI (5224) PacI (5210) T3 promoter T3 (5171 .. 5191) M13 rev lac operator lac promoter CAP binding site BspQI - SapI (4923) PciI (4806) pBR322ori-F (4651 .. 4670) ScaI (3437) MSCV-rev (247 .. 270) AgeI (322) SpeI (457) BlpI (485) mPGK-F (644 .. 663) BglII (726) Puro-R (751 .. 770) PflFI - Tth111I (793) PasI (800) RsrII (867) BstEII (885) SacII (965) CsiI - SexAI * (1318) BstAPI (1322) BspDI - ClaI (1405) BclI * (1424) BGH-rev (1431 .. 1448) BbsI (1626) PstI (1685) lac operator CAP binding site BamHI (1875) BsrGI (1993) NheI (2006) BmtI (2010) EcoRV (2023) AscI (2343) T7 (2358 .. 2377) M13 fwd M13/pUC Forward (2396 .. 2418) F1ori-R (2632 .. 2651) NgoMIV (2670) NaeI (2672) F1ori-F (2842 .. 2863) PsiI (2903) XmnI (3318) Amp-R (3349 .. 3368) PB-GG-OCT4-1-5-PGK-Puro 7493 bp

Sequence View

5ʹ 3ʹ 60 a g g a a c c c c t a g t g a t g g a g t t g g c c a c t c c c t c t c t g c g c g c t c g c t c g c t c a c t g a g g t c c t t g g g g a t c a c t a c c t c a a c c g g t g a g g g a g a g a c g c g c g a g c g a g c g a g t g a c t c c

120 loxP c c g g g c g a c c a a a g g t c g c c c g a c g c c c a a t t c c g a t c a t a t t c a a t a a c c c t t a a t a t a g g c c c g c t g g t t t c c a g c g g g c t g c g g g t t a a g g c t a g t a t a a g t t a t t g g g a a t t a t a t

180 loxP a c t t c g t a t a a t g t a t g c t a t a c g a a g t t a t t a g g t c t g a a g a g g a g t t t a c g t c c a g c c t g a a g c a t a t t a c a t a c g a t a t g c t t c a a t a a t c c a g a c t t c t c c t c a a a t g c a g g t c g g

240 PGK promoter a a g c t t a g g a t c t c g a c c t c g a a a t t c t a c c g g g t a g g g g a g g c g c t t t t c c c a a g g c a g t t c g a a t c c t a g a g c t g g a g c t t t a a g a t g g c c c a t c c c c t c c g c g a a a a g g g t t c c g t c

300 PGK promoter C G T A C G C G A A A T C G T C G G G G C G A C MSCV-rev t c t g g a g c a t g c g c t t t a g c a g c c c c g c t g g g c a c t t g g c g c t a c a c a a g t g g c c t c t g g a g a c c t c g t a c g c g a a a t c g t c g g g g c g a c c c g t g a a c c g c g a t g t g t t c a c c g g a g a c c

AgeI 360 PGK promoter c c t c g c a c a c a t t c c a c a t c c a c c g g t a g g c g c c a a c c g g c t c c g t t c t t t g g t g g c c c c g g a g c g t g t g t a a g g t g t a g g t g g c c a t c c g c g g t t g g c c g a g g c a a g a a a c c a c c g g g g

420 PGK promoter t t c g c g c c a c c t t c t a c t c c t c c c c t a g t c a g g a a g t t c c c c c c c g c c c c g c a g c t c g c g a a g c g c g g t g g a a g a t g a g g a g g g g a t c a g t c c t t c a a g g g g g g g c g g g g c g t c g a g c g c

SpeI 480 PGK promoter t c g t g c a g g a c g t g a c a a a t g g a a g t a g c a c g t c t c a c t a g t c t c g t g c a g a t g g a c a g c a g c a c g t c c t g c a c t g t t t a c c t t c a t c g t g c a g a g t g a t c a g a g c a c g t c t a c c t g t c g

BlpI 540 PGK promoter MEAGRPLGQRPIAALL a c c g c t g a g c a a t g g a a g c g g g t a g g c c t t t g g g g c a g c g g c c a a t a g c a g c t t t g c t c c t g g c g a c t c g t t a c c t t c g c c c a t c c g g a a a c c c c g t c g c c g g t t a t c g t c g a a a c g a g g

600 PGK promoter LRFLGSEAGKGWVRGRAQGR t t c g c t t t c t g g g c t c a g a g g c t g g g a a g g g g t g g g t c c g g g g g c g g g c t c a g g g g c g g g a a g c g a a a g a c c c g a g t c t c c g a c c c t t c c c c a c c c a g g c c c c c g c c c g a g t c c c c g c c c

660 PGK promoter C A T T C T G C A C G C T T C A A mPGK-F AQGRGGRPKVLRRPGILHAS c t c a g g g g c g g g g c g g g c g c c c g a a g g t c c t c c g g a g g c c c g g c a t t c t g c a c g c t t c a a g a g t c c c c g c c c c g c c c g c g g g c t t c c a g g a g g c c t c c g g g c c g t a a g a c g t g c g a a g t t

720 PGK promoter A A G mPGK-F KAHVCRAVLLFLISGPFDLH a a g c g c a c g t c t g c c g c g c t g t t c t c c t c t t c c t c a t c t c c g g g c c t t t c g a c c t g c a t c t t c g c g t g c a g a c g g c g c g a c a a g a g g a g a a g g a g t a g a g g c c c g g a a a g c t g g a c g t a g

BglII 780 1 5 10 MTEYKPTVRL PuroR T A C T G G C T C A T G T T C G G G T G Puro-R MTEYKPTVRL PSRSRAAEAYHDRVQAHGAP *IELLQLKGHGLVLGRHAE c a t c t a g a t c t c g a g c a g c t g a a g c t t a c c a t g a c c g a g t a c a a g c c c a c g g t g c g c c t c g t a g a t c t a g a g c t c g t c g a c t t c g a a t g g t a c t g g c t c a t g t t c g g g t g c c a c g c g g a g

PasITth111IPflFI 840 15 20 25 30 ATRDDVPRAVRTLAAAFADY PuroR ATRDDVPRAVRTLAAAFADY RHPRRRPQGRTHPRRRVRRL GGAVVDGPGYAGEGGREGVV g c c a c c c g c g a c g a c g t c c c c a g g g c c g t a c g c a c c c t c g c c g c c g c g t t c g c c g a c t a c c g g t g g g c g c t g c t g c a g g g g t c c c g g c a t g c g t g g g a g c g g c g g c g c a a g c g g c t g a t g

BstEIIRsrII 900 35 40 45 50 PATRHTVDPDRHIERVTELQ PuroR PATRHTVDPDRHIERVTELQ PRHAPHRRSGPPHRAGHRAA GGRAVGDIRVAVDLPDGLQL c c c g c c a c g c g c c a c a c c g t c g a t c c g g a c c g c c a c a t c g a g c g g g t c a c c g a g c t g c a a g g g c g g t g c g c g g t g t g g c a g c t a g g c c t g g c g g t g t a g c t c g c c c a g t g g c t c g a c g t t

960 55 60 65 70 ELFLTRVGLDIGKVWVADDG PuroR ELFLTRVGLDIGKVWVADDG RTLPHARRARHRQGVGRGRR FEEERADPEVDALHPDRVVA g a a c t c t t c c t c a c g c g c g t c g g g c t c g a c a t c g g c a a g g t g t g g g t c g c g g a c g a c g g c c t t g a g a a g g a g t g c g c g c a g c c c g a g c t g t a g c c g t t c c a c a c c c a g c g c c t g c t g c c g

SacII 1020 75 80 85 90 AAVAVWTTPESVEAGAVFAE PuroR AAVAVWTTPESVEAGAVFAE RRGGGLDHAGERRSGGGVRR GRHRDPGRRLADFRPRHEGL g c c g c g g t g g c g g t c t g g a c c a c g c c g g a g a g c g t c g a a g c g g g g g c g g t g t t c g c c g a g c g g c g c c a c c g c c a g a c c t g g t g c g g c c t c t c g c a g c t t c g c c c c c g c c a c a a g c g g c t c

1080 95 100 105 110 IGPRMAELSGSRLAAQQQME PuroR IGPRMAELSGSRLAAQQQME DRPAHGRVERFPAGRAATDG DARAHGLQATGPQGRLLLHF a t c g g c c c g c g c a t g g c c g a g t t g a g c g g t t c c c g g c t g g c c g c g c a g c a a c a g a t g g a a t a g c c g g g c g c g t a c c g g c t c a a c t c g c c a a g g g c c g a c c g g c g c g t c g t t g t c t a c c t t

1140 115 120 125 130 GLLAPHRPKEPAWFLATVGV PuroR GLLAPHRPKEPAWFLATVGV RPPGAAPAQGARVVPGHRRR AEQRRVPGLLGRPEQGGDAD g g c c t c c t g g c g c c g c a c c g g c c c a a g g a g c c c g c g t g g t t c c t g g c c a c c g t c g g c g t c c c g g a g g a c c g c g g c g t g g c c g g g t t c c t c g g g c g c a c c a a g g a c c g g t g g c a g c c g c a g

1200 135 140 145 150 SPDHQGKGLGSAVVLPGVEA PuroR SPDHQGKGLGSAVVLPGVEA LARPPGQGSGQRRRAPRSGG RGVVLALTQAAGDHEGSHLR t c g c c c g a c c a c c a g g g c a a g g g t c t g g g c a g c g c c g t c g t g c t c c c c g g a g t g g a g g c g a g c g g g c t g g t g g t c c c g t t c c c a g a c c c g t c g c g g c a g c a c g a g g g g c c t c a c c t c c g c

1260 155 160 165 170 AERAGVPAFLETSAPHNLPF PuroR AERAGVPAFLETSAPHNLPF GRARRGARLPGDLRAPQPPL GLAGPHGGEQLGGRGVVEGE g c c g a g c g c g c c g g g g t g c c c g c c t t c c t g g a g a c c t c c g c g c c c c a c a a c c t c c c c t t c c g g c t c g c g c g g c c c c a c g g g c g g a a g g a c c t c t g g a g g c g c g g g g t g t t g g a g g g g a a g

SexAI*CsiI 1320 175 180 185 190 YERLGFTVTADVEVPEGPRT PuroR YERLGFTVTADVEVPEGPRT LRAARLHRHRRRRGARRTAH VLPEAEGDGGVDLHGFSRAG t a c g a g c g g c t c g g c t t c a c c g t c a c c g c c g a c g t c g a g g t g c c c g a a g g a c c g c g c a c c a t g c t c g c c g a g c c g a a g t g g c a g t g g c g g c t g c a g c t c c a c g g g c t t c c t g g c g c g t g g

BstAPI 1380 195 200 WCMTRKPGA* PuroR WCMTRKPGA* LVHDPQARCLTPAPRPAAPD PAHGALGTGSARGVVRLARG t g g t g c a t g a c c c g c a a g c c c g g t g c c t g a c g c c c g c c c c a c g a c c c g c a g c g c c c g a c c a c c a c g t a c t g g g c g t t c g g g c c a c g g a c t g c g g g c g g g g t g c t g g g c g t c g c g g g c t g g

BclI*BspDIClaI 1440 bGH poly(A) signal G G A G C T G A C A BGH-rev RKERTTPCIDDLELADQPRL FPACSGM g a a a g g a g c g c a c g a c c c c a t g c a t c g a t g a t c t a g a g c t c g c t g a t c a g c c t c g a c t g t c t t t c c t c g c g t g c t g g g g t a c g t a g c t a c t a g a t c t c g a g c g a c t a g t c g g a g c t g a c a

1500 bGH poly(A) signal C G G A A G A T BGH-rev CLLVASHLLFAPPPCLP* g c c t t c t a g t t g c c a g c c a t c t g t t g t t t g c c c c t c c c c c g t g c c t t c c t t g a c c c t g g a c g g a a g a t c a a c g g t c g g t a g a c a a c a a a c g g g g a g g g g g c a c g g a a g g a a c t g g g a c c t

1560 bGH poly(A) signal a g g t g c c a c t c c c a c t g t c c t t t c c t a a t a a a a t g a g g a a a t t g c a t c g c a t t g t c t g a g t c c a c g g t g a g g g t g a c a g g a a a g g a t t a t t t t a c t c c t t t a a c g t a g c g t a a c a g a c t c

1620 bGH poly(A) signal t a g g t g t c a t t c t a t t c t g g g g g g t g g g g t g g g g c a g g a c a g c a a g g g g g a g g a t t g g g a a t c c a c a g t a a g a t a a g a c c c c c c a c c c c a c c c c g t c c t g t c g t t c c c c c t c c t a a c c c t

BbsI 1680 bGH poly(A) signal a g a c a a t a g c a g g c a t g c t g g g g a t g c g g t g g g c t c t a t g g c t t c t g a g g c g g a a a g a a c t c t g t t a t c g t c c g t a c g a c c c c t a c g c c a c c c g a g a t a c c g a a g a c t c c g c c t t t c t t g

PstI 1740 M13 rev lac operator c t g c a g c c c a a g c t t g g c g t a a t c a t g g t c a t a g c t g t t t c c t g t g t g a a a t t g t t a t c c g a c g t c g g g t t c g a a c c g c a t t a g t a c c a g t a t c g a c a a a g g a c a c a c t t t a a c a a t a g g

1800 -10 -35 lac operator lac promoter g c t c a c a a t t c c a c a c a a c a t a c g a g c c g g a a g c a t a a a g t g t a a a g c c t g g g g t g c c t a c g a g t g t t a a g g t g t g t t g t a t g c t c g g c c t t c g t a t t t c a c a t t t c g g a c c c c a c g g a t

1860 CAP binding site a t g a g t g a g c t a a c t c a c a t t a a t t g c g t t g c g c t c a c t g c c c g c t t t c c a g t c g g g a a a t a c t c a c t c g a t t g a g t g t a a t t a a c g c a a c g c g a g t g a c g g g c g a a a g g t c a g c c c t t t

BamHI 1920 attR1 c c t g t c g t g c c a g c g g a t c c c a t a g t g a c t g g a t a t g t t g t g t t t t a c a g t a t t a t g t a g g g a c a g c a c g g t c g c c t a g g g t a t c a c t g a c c t a t a c a a c a c a a a a t g t c a t a a t a c a t c

1980 attR1 t c t g t t t t t t a t g c a a a a t c t a a t t t a a t a t a t t g a t a t t t a t a t c a t t t t a c g t t t c t c a g a c a a a a a a t a c g t t t t a g a t t a a a t t a t a t a a c t a t a a a t a t a g t a a a a t g c a a a g a g

EcoRVBmtINheIBsrGI 2040 attR1 g t t c a g c t t t t t t g t a c a a a c t t g t g c t a g c a t t c t a g t t g a t a t c t a t a a c a a g a a a a t c a a g t c g a a a a a a c a t g t t t g a a c a c g a t c g t a a g a t c a a c t a t a g a t a t t g t t c t t t t a

2100 a t a t a t a t a a t a a g t t a t c a c g t a a g t a g a a c a t g a a a t a a c a a t a t a a t t a t c g t a t g a t a t a t a t a t t a t t c a a t a g t g c a t t c a t c t t g t a c t t t a t t g t t a t a t t a a t a g c a t a c t

2160 g t t a a a t c t t a a a a g t c a c g t a a a a g a t a a t c a t g c g t c a t t t t g a c t c a c g c g g t c g t t c a a t t t a g a a t t t t c a g t g c a t t t t c t a t t a g t a c g c a g t a a a a c t g a g t g c g c c a g c a a

2220 a t a g t t c a a a a t c a g t g a c a c t t a c c g c a t t g a c a a g c a c g c c t c a c g g g a g c t c c a a g c t a t c a a g t t t t a g t c a c t g t g a a t g g c g t a a c t g t t c g t g c g g a g t g c c c t c g a g g t t c g

2280 g g c g a c t g a g a t g t c c t a a a t g c a c a g c g a c g g a t t c g c g c t a t t t a g a a a g a g a g a g c a c c g c t g a c t c t a c a g g a t t t a c g t g t c g c t g c c t a a g c g c g a t a a a t c t t t c t c t c t c g t

2340 piggyBac left (5') inverted repeat a t a t t t c a a g a a t g c a t g c g t c a a t t t t a c g c a g a c t a t c t t t c t a g g g t t a a t c t a g a a t a t a a a g t t c t t a c g t a c g c a g t t a a a a t g c g t c t g a t a g a a a g a t c c c a a t t a g a t c t t

AscI 2400 T7 promoter M13 fwd G G G A T A T C A C T C A G C A T A A T T7 G C A A A M13/pUC Forward t g g c g c g c c c c a a t t c g c c c t a t a g t g a g t c g t a t t a c g c g c g c t c a c t g g c c g t c g t t t a c c g c g c g g g g t t a a g c g g g a t a t c a c t c a g c a t a a t g c g c g c g a g t g a c c g g c a g c a a a

2460 M13 fwd A T G T T G C A G C A C T G A C C C M13/pUC Forward t a c a a c g t c g t g a c t g g g a a a a c c c t g g c g t t a c c c a a c t t a a t c g c c t t g c a g c a c a t c a t g t t g c a g c a c t g a c c c t t t t g g g a c c g c a a t g g g t t g a a t t a g c g g a a c g t c g t g t a g

2520 c c c c t t t c g c c a g c t g g c g t a a t a g c g a a g a g g c c c g c a c c g a t c g c c c t t c c c a a c a g t g g g g a a a g c g g t c g a c c g c a t t a t c g c t t c t c c g g g c g t g g c t a g c g g g a a g g g t t g t c a

2580 f1 ori t g c g c a g c c t g a a t g g c g a a t g g g a c g c g c c c t g t a g c g g c g c a t t a a g c g c g g c g g g t g a c g c g t c g g a c t t a c c g c t t a c c c t g c g c g g g a c a t c g c c g c g t a a t t c g c g c c g c c c a c

2640 f1 ori G A G G A A A G C F1ori-R t g g t g g t t a c g c g c a g c g t g a c c g c t a c a c t t g c c a g c g c c c t a g c g c c c g c t c c t t t c g a c c a c c a a t g c g c g t c g c a c t g g c g a t g t g a a c g g t c g c g g g a t c g c g g g c g a g g a a a g c

NgoMIVNaeI 2700 f1 ori G A A A G A A G G G A F1ori-R c t t t c t t c c c t t c c t t t c t c g c c a c g t t c g c c g g c t t t c c c c g t c a a g c t c t a a a t c g g g g a a a g a a g g g a a g g a a a g a g c g g t g c a a g c g g c c g a a a g g g g c a g t t c g a g a t t t a g c c c

2760 f1 ori g g c t c c c t t t a g g g t t c c g a t t t a g t g c t t t a c g g c a c c t c g a c c c c a a a a a a c t t g a t t c c g a g g g a a a t c c c a a g g c t a a a t c a c g a a a t g c c g t g g a g c t g g g g t t t t t t g a a c t a a

2820 f1 ori a g g g t g a t g g t t c a c g t a g t g g g c c a t c g c c c t g a t a g a c g g t t t t t c g c c c t t t g a c g t t c c c a c t a c c a a g t g c a t c a c c c g g t a g c g g g a c t a t c t g c c a a a a a g c g g g a a a c t g c a

2880 f1 ori G T G G A C T C T T G T T C C A A A C T G G F1ori-F t g g a g t c c a c g t t c t t t a a t a g t g g a c t c t t g t t c c a a a c t g g a a c a a c a c t c a a c c c t a a c c t c a g g t g c a a g a a a t t a t c a c c t g a g a a c a a g g t t t g a c c t t g t t g t g a g t t g g g a t

PsiI 2940 f1 ori t c t c g g t c t a t t c t t t t g a t t t a t a a g g g a t t t t g c c g a t t t c g g c c t a t t g g t t a a a a a a g a g c c a g a t a a g a a a a c t a a a t a t t c c c t a a a a c g g c t a a a g c c g g a t a a c c a a t t t t t

3000 f1 ori a t g a g c t g a t t t a a c a a a a a t t t a a c g c g a a t t t t a a c a a a a t a t t a a c g c t t a c a a t t t t a c t c g a c t a a a t t g t t t t t a a a t t g c g c t t a a a a t t g t t t t a t a a t t g c g a a t g t t a a a

3060 AmpR promoter a g g t g g c a c t t t t c g g g g a a a t g t g c g c g g a a c c c c t a t t t g t t t a t t t t t c t a a a t a c a t c c a c c g t g a a a a g c c c c t t t a c a c g c g c c t t g g g g a t a a a c a a a t a a a a a g a t t t a t g t

3120 AmpR promoter t t c a a a t a t g t a t c c g c t c a t g a g a c a a t a a c c c t g a t a a a t g c t t c a a t a a t a t t g a a a a a g t t t a t a c a t a g g c g a g t a c t c t g t t a t t g g g a c t a t t t a c g a a g t t a t t a t a a c t t t

3180 1 5 10 15 signal sequence MSIQHFRVALIPFFAAF AmpR promoter AmpR MSIQHFRVALIPFFAAF a a g g a a g a g t a t g a g t a t t c a a c a t t t c c g t g t c g c c c t t a t t c c c t t t t t t g c g g c a t t t t c c t t c t c a t a c t c a t a a g t t g t a a a g g c a c a g c g g g a a t a a g g g a a a a a a c g c c g t a a

3240 20 signal sequence CLPVFA 25 30 35 HPETLVKVKDAEDQ AmpR CLPVFAHPETLVKVKDAEDQ t t g c c t t c c t g t t t t t g c t c a c c c a g a a a c g c t g g t g a a a g t a a a a g a t g c t g a a g a t c a a a c g g a a g g a c a a a a a c g a g t g g g t c t t t g c g a c c a c t t t c a t t t t c t a c g a c t t c t a g t

3300 40 45 50 55 LGARVGYIELDLNSGKILES AmpR LGARVGYIELDLNSGKILES g t t g g g t g c a c g a g t g g g t t a c a t c g a a c t g g a t c t c a a c a g c g g t a a g a t c c t t g a g a g c a a c c c a c g t g c t c a c c c a a t g t a g c t t g a c c t a g a g t t g t c g c c a t t c t a g g a a c t c t c

XmnI 3360 60 65 70 75 FRPEERFPMMSTFKVLLCGA AmpR C G A T A C A C C G C G Amp-R FRPEERFPMMSTFKVLLCGA t t t t c g c c c c g a a g a a c g t t t t c c a a t g a t g a g c a c t t t t a a a g t t c t g c t a t g t g g c g c a a a a g c g g g g c t t c t t g c a a a a g g t t a c t a c t c g t g a a a a t t t c a a g a c g a t a c a c c g c g

3420 80 85 90 95 VLSRIDAGQEQLGRRIHYSQ AmpR C C A T A A T A Amp-R VLSRIDAGQEQLGRRIHYSQ g g t a t t a t c c c g t a t t g a c g c c g g g c a a g a g c a a c t c g g t c g c c g c a t a c a c t a t t c t c a c c a t a a t a g g g c a t a a c t g c g g c c c g t t c t c g t t g a g c c a g c g g c g t a t g t g a t a a g a g t

ScaI 3480 100 105 110 115 NDLVEYSPVTEKHLTDGMTV AmpR NDLVEYSPVTEKHLTDGMTV g a a t g a c t t g g t t g a g t a c t c a c c a g t c a c a g a a a a g c a t c t t a c g g a t g g c a t g a c a g t c t t a c t g a a c c a a c t c a t g a g t g g t c a g t g t c t t t t c g t a g a a t g c c t a c c g t a c t g t c a

3540 120 125 130 135 RELCSAAITMSDNTAANLLL AmpR RELCSAAITMSDNTAANLLL a a g a g a a t t a t g c a g t g c t g c c a t a a c c a t g a g t g a t a a c a c t g c g g c c a a c t t a c t t c t t t c t c t t a a t a c g t c a c g a c g g t a t t g g t a c t c a c t a t t g t g a c g c c g g t t g a a t g a a g a

3600 140 145 150 155 TTIGGPKELTAFLHNMGDHV AmpR TTIGGPKELTAFLHNMGDHV g a c a a c g a t c g g a g g a c c g a a g g a g c t a a c c g c t t t t t t g c a c a a c a t g g g g g a t c a t g t c t g t t g c t a g c c t c c t g g c t t c c t c g a t t g g c g a a a a a a c g t g t t g t a c c c c c t a g t a c a

3660 160 165 170 175 TRLDRWEPELNEAIPNDERD AmpR TRLDRWEPELNEAIPNDERD a a c t c g c c t t g a t c g t t g g g a a c c g g a g c t g a a t g a a g c c a t a c c a a a c g a c g a g c g t g a t t g a g c g g a a c t a g c a a c c c t t g g c c t c g a c t t a c t t c g g t a t g g t t t g c t g c t c g c a c t

3720 180 185 190 195 TTMPVAMATTLRKLLTGELL AmpR TTMPVAMATTLRKLLTGELL c a c c a c g a t g c c t g t a g c a a t g g c a a c a a c g t t g c g c a a a c t a t t a a c t g g c g a a c t a c t g t g g t g c t a c g g a c a t c g t t a c c g t t g t t g c a a c g c g t t t g a t a a t t g a c c g c t t g a t g a

3780 200 205 210 215 TLASRQQLIDWMEADKVAGP AmpR TLASRQQLIDWMEADKVAGP t a c t c t a g c t t c c c g g c a a c a a t t a a t a g a c t g g a t g g a g g c g g a t a a a g t t g c a g g a c c a t g a g a t c g a a g g g c c g t t g t t a a t t a t c t g a c c t a c c t c c g c c t a t t t c a a c g t c c t g g

3840 220 225 230 235 LLRSALPAGWFIADKSGAGE AmpR LLRSALPAGWFIADKSGAGE a c t t c t g c g c t c g g c c c t t c c g g c t g g c t g g t t t a t t g c t g a t a a a t c t g g a g c c g g t g a t g a a g a c g c g a g c c g g g a a g g c c g a c c g a c c a a a t a a c g a c t a t t t a g a c c t c g g c c a c t

3900 240 245 250 255 RGSRGIIAALGPDGKPSRIV AmpR RGSRGIIAALGPDGKPSRIV g c g t g g g t c t c g c g g t a t c a t t g c a g c a c t g g g g c c a g a t g g t a a g c c c t c c c g t a t c g t c g c a c c c a g a g c g c c a t a g t a a c g t c g t g a c c c c g g t c t a c c a t t c g g g a g g g c a t a g c a

3960 260 265 270 275 VIYTTGSQATMDERNRQIAE AmpR VIYTTGSQATMDERNRQIAE a g t t a t c t a c a c g a c g g g g a g t c a g g c a a c t a t g g a t g a a c g a a a t a g a c a g a t c g c t g a t c a a t a g a t g t g c t g c c c c t c a g t c c g t t g a t a c c t a c t t g c t t t a t c t g t c t a g c g a c t

4020 280 285 IGASLIKHW* AmpR IGASLIKHW* g a t a g g t g c c t c a c t g a t t a a g c a t t g g t a a c t g t c a g a c c a a g t t t a c t c a t a t a t a c t c t a t c c a c g g a g t g a c t a a t t c g t a a c c a t t g a c a g t c t g g t t c a a a t g a g t a t a t a t g a

4080 t t a g a t t g a t t t a a a a c t t c a t t t t t a a t t t a a a a g g a t c t a g g t g a a g a t c c t t t t t g a a a t c t a a c t a a a t t t t g a a g t a a a a a t t a a a t t t t c c t a g a t c c a c t t c t a g g a a a a a c t

4140 t a a t c t c a t g a c c a a a a t c c c t t a a c g t g a g t t t t c g t t c c a c t g a g c g t c a g a c c c c g t a t t a g a g t a c t g g t t t t a g g g a a t t g c a c t c a a a a g c a a g g t g a c t c g c a g t c t g g g g c a

4200 ori a g a a a a g a t c a a a g g a t c t t c t t g a g a t c c t t t t t t t c t g c g c g t a a t c t g c t g c t t g c a t c t t t t c t a g t t t c c t a g a a g a a c t c t a g g a a a a a a a g a c g c g c a t t a g a c g a c g a a c g t

4260 ori a a c a a a a a a a c c a c c g c t a c c a g c g g t g g t t t g t t t g c c g g a t c a a g a g c t a c c a a c t c t t t g t t t t t t t g g t g g c g a t g g t c g c c a c c a a a c a a a c g g c c t a g t t c t c g a t g g t t g a g a

4320 ori t t t t c c g a a g g t a a c t g g c t t c a g c a g a g c g c a g a t a c c a a a t a c t g t t c t t c t a g t g t a a a a a g g c t t c c a t t g a c c g a a g t c g t c t c g c g t c t a t g g t t t a t g a c a a g a a g a t c a c a t

4380 ori g c c g t a g t t a g g c c a c c a c t t c a a g a a c t c t g t a g c a c c g c c t a c a t a c c t c g c t c t g c t c g g c a t c a a t c c g g t g g t g a a g t t c t t g a g a c a t c g t g g c g g a t g t a t g g a g c g a g a c g a

4440 ori a a t c c t g t t a c c a g t g g c t g c t g c c a g t g g c g a t a a g t c g t g t c t t a c c g g g t t g g a c t c t t a g g a c a a t g g t c a c c g a c g a c g g t c a c c g c t a t t c a g c a c a g a a t g g c c c a a c c t g a g

4500 ori a a g a c g a t a g t t a c c g g a t a a g g c g c a g c g g t c g g g c t g a a c g g g g g g t t c g t g c a c a c a t t c t g c t a t c a a t g g c c t a t t c c g c g t c g c c a g c c c g a c t t g c c c c c c a a g c a c g t g t g t

4560 ori g c c c a g c t t g g a g c g a a c g a c c t a c a c c g a a c t g a g a t a c c t a c a g c g t g a g c t a t g a g a c g g g t c g a a c c t c g c t t g c t g g a t g t g g c t t g a c t c t a t g g a t g t c g c a c t c g a t a c t c t

4620 ori a a g c g c c a c g c t t c c c g a a g g g a g a a a g g c g g a c a g g t a t c c g g t a a g c g g c a g g g t c g g t t c g c g g t g c g a a g g g c t t c c c t c t t t c c g c c t g t c c a t a g g c c a t t c g c c g t c c c a g c c

4680 ori G G G A A A C G C C T G G T A T C T T T pBR322ori-F a a c a g g a g a g c g c a c g a g g g a g c t t c c a g g g g g a a a c g c c t g g t a t c t t t a t a g t c c t g t t t g t c c t c t c g c g t g c t c c c t c g a a g g t c c c c c t t t g c g g a c c a t a g a a a t a t c a g g a c a

4740 ori c g g g t t t c g c c a c c t c t g a c t t g a g c g t c g a t t t t t g t g a t g c t c g t c a g g g g g g c g g a g g c c c a a a g c g g t g g a g a c t g a a c t c g c a g c t a a a a a c a c t a c g a g c a g t c c c c c c g c c t c

4800 ori c c t a t g g a a a a a c g c c a g c a a c g c g g c c t t t t t a c g g t t c c t g g c c t t t t g c t g g c c t t t g g a t a c c t t t t t g c g g t c g t t g c g c c g g a a a a a t g c c a a g g a c c g g a a a a c g a c c g g a a a

PciI 4860 t g c t c a c a t g t t c t t t c c t g c g t t a t c c c c t g a t t c t g t g g a t a a c c g t a t t a c c g c c t t a c g a g t g t a c a a g a a a g g a c g c a a t a g g g g a c t a a g a c a c c t a t t g g c a t a a t g g c g g a a

4920 t g a g t g a g c t g a t a c c g c t c g c c g c a g c c g a a c g a c c g a g c g c a g c g a g t c a g t g a g c g a a c t c a c t c g a c t a t g g c g a g c g g c g t c g g c t t g c t g g c t c g c g t c g c t c a g t c a c t c g c t

BspQISapI 4980 g g a a g c g g a a g a g c g c c c a a t a c g c a a a c c g c c t c t c c c c g c g c g t t g g c c g a t t c a t t a c c t t c g c c t t c t c g c g g g t t a t g c g t t t g g c g g a g a g g g g c g c g c a a c c g g c t a a g t a a t

5040 CAP binding site a t g c a g c t g g c a c g a c a g g t t t c c c g a c t g g a a a g c g g g c a g t g a g c g c a a c g c a a t t a a t a c g t c g a c c g t g c t g t c c a a a g g g c t g a c c t t t c g c c c g t c a c t c g c g t t g c g t t a a t t

5100 -35 -10 CAP binding site lac promoter t g t g a g t t a g c t c a c t c a t t a g g c a c c c c a g g c t t t a c a c t t t a t g c t t c c g g c t c g t a t a c a c t c a a t c g a g t g a g t a a t c c g t g g g g t c c g a a a t g t g a a a t a c g a a g g c c g a g c a t a

5160 -10 lac promoter lac operator M13 rev g t t g t g t g g a a t t g t g a g c g g a t a a c a a t t t c a c a c a g g a a a c a g c t a t g a c c a t g a t t a c a a c a c a c c t t a a c a c t c g c c t a t t g t t a a a g t g t g t c c t t t g t c g a t a c t g g t a c t a a t

PacI 5220 T3 promoter G C A A T T A A C C C T C A C T A A A G G T3 c g c c a a g c g c g c a a t t a a c c c t c a c t a a a g g g a a c a a a a g c t g g a t t a a t t a a g c g g c c g g c g g t t c g c g c g t t a a t t g g g a g t g a t t t c c c t t g t t t t c g a c c t a a t t a a t t c g c c g g c

MluIAccIBstZ17I 5280 piggyBac right (3') inverted repeat c a t a c g c g t a t a c t a g a t t a a c c c t a g a a a g a t a a t c a t a t t g t g a c g t a c g t t a a a g a t g t a t g c g c a t a t g a t c t a a t t g g g a t c t t t c t a t t a g t a t a a c a c t g c a t g c a a t t t c t a

5340 piggyBac right (3') inverted repeat a a t c a t g c g t a a a a t t g a c g c a t g t g t t t t a t c g g t c t g t a t a t c g a g g t t t a t t t a t t a t t a g t a c g c a t t t t a a c t g c g t a c a c a a a a t a g c c a g a c a t a t a g c t c c a a a t a a a t a a t

5400 a t t t g a a t a g a t a t t a a g t t t t a t t a t a t t t a c a c t t a c a t a c t a a t a a t a a a t t c a a c a t a a a c t t a t c t a t a a t t c a a a a t a a t a t a a a t g t g a a t g t a t g a t t a t t a t t t a a g t t g t

5460 a a c a a t t t a t t t a t g t t t a t t t a t t t a t t a a a a a a a a a c a a a a a c t c a a a a t t t c t t c t a t t g t t a a a t a a a t a c a a a t a a a t a a a t a a t t t t t t t t t g t t t t t g a g t t t t a a a g a a g a t

BstBIEcoRI 5520 M13 fwd t a a a g t a a c a a a a c t t t t a a c t a g a g g a t c t c g a g c t c a a g c t t c g a a t t c c c c c t g t a a a t t t c a t t g t t t t g a a a a t t g a t c t c c t a g a g c t c g a g t t c g a a g c t t a a g g g g g a c a t t

5580 M13 fwd U6 promoter a a c g a c g g c c a g t g a g g g c c t a t t t c c c a t g a t t c c t t c a t a t t t g c a t a t a c g a t a c a a t t g c t g c c g g t c a c t c c c g g a t a a a g g g t a c t a a g g a a g t a t a a a c g t a t a t g c t a t g t t

5640 U6 promoter g g c t g t t a g a g a g a t a a t t g g a a t t a a t t t g a c t g t a a a c a c a a a g a t a t t a g t a c a a a a c c g a c a a t c t c t c t a t t a a c c t t a a t t a a a c t g a c a t t t g t g t t t c t a t a a t c a t g t t t t

5700 U6 promoter t a c g t g a c g t a g a a a g t a a t a a t t t c t t g g g t a g t t t g c a g t t t t a a a a t t a t g t t t t a a a t g c a c t g c a t c t t t c a t t a t t a a a g a a c c c a t c a a a c g t c a a a a t t t t a a t a c a a a a t t

5760 U6 promoter a a t g g a c t a t c a t a t g c t t a c c g t a a c t t g a a a g t a t t t c g a t t t c t t g g c t t t a t a t a t t t a c c t g a t a g t a t a c g a a t g g c a t t g a a c t t t c a t a a a g c t a a a g a a c c g a a a t a t a t a

5820 U6 promoter gRNA scaffold c t t g t g g a a a g g a c g a a a c a c c g g g g g g a g a a a c t g a g g c g a g t t t t a g a g c t a g a a a t a g a a c a c c t t t c c t g c t t t g t g g c c c c c c t c t t t g a c t c c g c t c a a a a t c t c g a t c t t t a t

5880 gRNA scaffold g c a a g t t a a a a t a a g g c t a g t c c g t t a t c a a c t t g a a a a a g t g g c a c c g a g t c g g t g c t t c g t t c a a t t t t a t t c c g a t c a g g c a a t a g t t g a a c t t t t t c a c c g t g g c t c a g c c a c g a a

5940 M13 fwd U6 promoter t t t t t c a t g g t c a t a g c t g t t t c c t c a t g g t a a a a c g a c g g c c a g t g a g g g c c t a t t t c c a a a a a g t a c c a g t a t c g a c a a a g g a g t a c c a t t t t g c t g c c g g t c a c t c c c g g a t a a a g g

6000 U6 promoter c a t g a t t c c t t c a t a t t t g c a t a t a c g a t a c a a g g c t g t t a g a g a g a t a a t t g g a a t t a a g t a c t a a g g a a g t a t a a a c g t a t a t g c t a t g t t c c g a c a a t c t c t c t a t t a a c c t t a a t t

6060 U6 promoter t t t g a c t g t a a a c a c a a a g a t a t t a g t a c a a a a t a c g t g a c g t a g a a a g t a a t a a t t t c t a a a c t g a c a t t t g t g t t t c t a t a a t c a t g t t t t a t g c a c t g c a t c t t t c a t t a t t a a a g a

6120 U6 promoter t g g g t a g t t t g c a g t t t t a a a a t t a t g t t t t a a a a t g g a c t a t c a t a t g c t t a c c g t a a c a c c c a t c a a a c g t c a a a a t t t t a a t a c a a a a t t t t a c c t g a t a g t a t a c g a a t g g c a t t g

6180 U6 promoter t t g a a a g t a t t t c g a t t t c t t g g c t t t a t a t a t c t t g t g g a a a g g a c g a a a c a c c g g g t g a a c t t t c a t a a a g c t a a a g a a c c g a a a t a t a t a g a a c a c c t t t c c t g c t t t g t g g c c c a c

6240 gRNA scaffold g t g g c a a t g g t g t c t g g t t t t a g a g c t a g a a a t a g c a a g t t a a a a t a a g g c t a g t c c g t t c a c c g t t a c c a c a g a c c a a a a t c t c g a t c t t t a t c g t t c a a t t t t a t t c c g a t c a g g c a a

6300 gRNA scaffold M13 rev a t c a a c t t g a a a a a g t g g c a c c g a g t c g g t g c t t t t t t t c a t g g t c a t a g c t g t t t c c t g t a g t t g a a c t t t t t c a c c g t g g c t c a g c c a c g a a a a a a a g t a c c a g t a t c g a c a a a g g a c

6360 M13 fwd U6 promoter g a c g t a a a a c g a c g g c c a g t g a g g g c c t a t t t c c c a t g a t t c c t t c a t a t t t g c a t a t a c c t g c a t t t t g c t g c c g g t c a c t c c c g g a t a a a g g g t a c t a a g g a a g t a t a a a c g t a t a t g

6420 U6 promoter g a t a c a a g g c t g t t a g a g a g a t a a t t g g a a t t a a t t t g a c t g t a a a c a c a a a g a t a t t a g c t a t g t t c c g a c a a t c t c t c t a t t a a c c t t a a t t a a a c t g a c a t t t g t g t t t c t a t a a t c

6480 U6 promoter t a c a a a a t a c g t g a c g t a g a a a g t a a t a a t t t c t t g g g t a g t t t g c a g t t t t a a a a t t a t a t g t t t t a t g c a c t g c a t c t t t c a t t a t t a a a g a a c c c a t c a a a c g t c a a a a t t t t a a t a

6540 U6 promoter g t t t t a a a a t g g a c t a t c a t a t g c t t a c c g t a a c t t g a a a g t a t t t c g a t t t c t t g g c t t c a a a a t t t t a c c t g a t a g t a t a c g a a t g g c a t t g a a c t t t c a t a a a g c t a a a g a a c c g a a

6600 U6 promoter t a t a t a t c t t g t g g a a a g g a c g a a a c a c c g g a c a c a a c t g g c g c c c c t c c g t t t t a g a g c a t a t a t a g a a c a c c t t t c c t g c t t t g t g g c c t g t g t t g a c c g c g g g g a g g c a a a a t c t c g

6660 t a g a a a t a g c a a g t t a a a a t a a g g c t a g t c c g t t a t c a a c t t g a a a a a g t g g c a c c g a g t a t c t t t a t c g t t c a a t t t t a t t c c g a t c a g g c a a t a g t t g a a c t t t t t c a c c g t g g c t c a

6720 M13 fwd U6 promoter c g t g c t t t t t t t c a t g g t c a t a g c t g t t t c c t c c a g g t a a a a c g a c g g c c a g t g a g g g c c g c a c g a a a a a a a g t a c c a g t a t c g a c a a a g g a g g t c c a t t t t g c t g c c g g t c a c t c c c g g

6780 U6 promoter t a t t t c c c a t g a t t c c t t c a t a t t t g c a t a t a c g a t a c a a g g c t g t t a g a g a g a t a a t t g a t a a a g g g t a c t a a g g a a g t a t a a a c g t a t a t g c t a t g t t c c g a c a a t c t c t c t a t t a a c

6840 U6 promoter g a a t t a a t t t g a c t g t a a a c a c a a a g a t a t t a g t a c a a a a t a c g t g a c g t a g a a a g t a a t c t t a a t t a a a c t g a c a t t t g t g t t t c t a t a a t c a t g t t t t a t g c a c t g c a t c t t t c a t t a

6900 U6 promoter a a t t t c t t g g g t a g t t t g c a g t t t t a a a a t t a t g t t t t a a a a t g g a c t a t c a t a t g c t t a t t a a a g a a c c c a t c a a a c g t c a a a a t t t t a a t a c a a a a t t t t a c c t g a t a g t a t a c g a a t

6960 U6 promoter c c g t a a c t t g a a a g t a t t t c g a t t t c t t g g c t t t a t a t a t c t t g t g g a a a g g a c g a a a c a g g c a t t g a a c t t t c a t a a a g c t a a a g a a c c g a a a t a t a t a g a a c a c c t t t c c t g c t t t g t

BstXI 7020 U6 promoter gRNA scaffold c c g g g c a c a g t g c c a g a g g t c t g g t t t t a g a g c t a g a a a t a g c a a g t t a a a a t a a g g c t a g g c c c g t g t c a c g g t c t c c a g a c c a a a a t c t c g a t c t t t a t c g t t c a a t t t t a t t c c g a t

7080 gRNA scaffold g t c c g t t a t c a a c t t g a a a a a g t g g c a c c g a g t c g g t g c t t t t t t t c a t g g t c a t a g c t g c a g g c a a t a g t t g a a c t t t t t c a c c g t g g c t c a g c c a c g a a a a a a a g t a c c a g t a t c g a c

7140 M13 fwd U6 promoter t t t c c t t g t t g t a a a a c g a c g g c c a g t g a g g g c c t a t t t c c c a t g a t t c c t t c a t a t t t g a a a g g a a c a a c a t t t t g c t g c c g g t c a c t c c c g g a t a a a g g g t a c t a a g g a a g t a t a a a c

7200 U6 promoter c a t a t a c g a t a c a a g g c t g t t a g a g a g a t a a t t g g a a t t a a t t t g a c t g t a a a c a c a a a g g t a t a t g c t a t g t t c c g a c a a t c t c t c t a t t a a c c t t a a t t a a a c t g a c a t t t g t g t t t c

7260 U6 promoter a t a t t a g t a c a a a a t a c g t g a c g t a g a a a g t a a t a a t t t c t t g g g t a g t t t g c a g t t t t a t a t a a t c a t g t t t t a t g c a c t g c a t c t t t c a t t a t t a a a g a a c c c a t c a a a c g t c a a a a t

7320 U6 promoter a a a t t a t g t t t t a a a a t g g a c t a t c a t a t g c t t a c c g t a a c t t g a a a g t a t t t c g a t t t c t t t a a t a c a a a a t t t t a c c t g a t a g t a t a c g a a t g g c a t t g a a c t t t c a t a a a g c t a a a g

7380 U6 promoter gRNA scaffold t t g g c t t t a t a t a t c t t g t g g a a a g g a c g a a a c a c c g t c t g t g g g g g a c c t g c a c t g g t t a a c c g a a a t a t a t a g a a c a c c t t t c c t g c t t t g t g g c a g a c a c c c c c t g g a c g t g a c c a a

7440 gRNA scaffold t t a g a g c t a g a a a t a g c a a g t t a a a a t a a g g c t a g t c c g t t a t c a a c t t g a a a a a g t g g c a a t c t c g a t c t t t a t c g t t c a a t t t t a t t c c g a t c a g g c a a t a g t t g a a c t t t t t c a c c g

3ʹ 5ʹ 7493 gRNA scaffold a c c g a g t c g g t g c t t t t t t t c a t g g t c a t a g c t g t t t c c t a a c g a g c g g c c g c t g g c t c a g c c a c g a a a a a a a g t a c c a g t a t c g a c a a a g g a t t g c t c g c c g g c g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.