Addgene pPHR-EYFP-hGCN5 Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pPHR-EYFP-hGCN5 Sequencing Result


Map View

PciI (8625) pBR322ori-F (8470 .. 8489) ApaLI (8311) TK-pA-R (7561 .. 7580) BstBI (7389) Neo-F (7243 .. 7262) RsrII (7223) PflFI - Tth111I (6825) Neo-R (6633 .. 6652) BspDI * - ClaI * (6547) SV40 promoter CsiI - SexAI * (6296) pBABE 3' (6210 .. 6230) F1ori-R (5948 .. 5967) DraIII (5823) F1ori-F (5736 .. 5757) AflII (5589) EBV-rev (5562 .. 5581) SV40pA-R (5508 .. 5527) MfeI (5457) XbaI * (5361) AhdI (5357) ScaI (5286) NruI (5260) PflMI * (5095) BlpI (4923) BclI * (4504) SacII (4334) AseI (0) NdeI (227) SnaBI (333) CMV-F (512 .. 532) NheI (584) BmtI (588) AfeI (589) PaeR7I - XhoI (606) XcmI (620) EcoRI (1340) EGFP-N (2166 .. 2187) EXFP-R (2427 .. 2446) EGFP-C (2774 .. 2795) BsrGI (2830) NotI (2840) PspOMI (3030) ApaI (3034) Eco53kI (3614) SacI (3616) PaqCI (3671) BbvCI (3691) BmgBI (3698) PmlI (3746) EcoNI (4132) BsmBI - Esp3I (4266) pPHR-EYFP-hGCN5 8690 bp

Sequence View

AseI 5ʹ 3ʹ 60 CMV enhancer t a a t a g t a a t c a a t t a c g g g g t c a t t a g t t c a t a g c c c a t a t a t g g a g t t c c g c g t t a c a a t t a t c a t t a g t t a a t g c c c c a g t a a t c a a g t a t c g g g t a t a t a c c t c a a g g c g c a a t g t

120 CMV enhancer t a a c t t a c g g t a a a t g g c c c g c c t g g c t g a c c g c c c a a c g a c c c c c g c c c a t t g a c g t c a a t t g a a t g c c a t t t a c c g g g c g g a c c g a c t g g c g g g t t g c t g g g g g c g g g t a a c t g c a g t

180 CMV enhancer a t a a t g a c g t a t g t t c c c a t a g t a a c g c c a a t a g g g a c t t t c c a t t g a c g t c a a t g g g t g t a t t a c t g c a t a c a a g g g t a t c a t t g c g g t t a t c c c t g a a a g g t a a c t g c a g t t a c c c a c

NdeI 240 CMV enhancer g a g t a t t t a c g g t a a a c t g c c c a c t t g g c a g t a c a t c a a g t g t a t c a t a t g c c a a g t a c g c t c a t a a a t g c c a t t t g a c g g g t g a a c c g t c a t g t a g t t c a c a t a g t a t a c g g t t c a t g c

300 CMV enhancer c c c c c t a t t g a c g t c a a t g a c g g t a a a t g g c c c g c c t g g c a t t a t g c c c a g t a c a t g a c c g g g g g a t a a c t g c a g t t a c t g c c a t t t a c c g g g c g g a c c g t a a t a c g g g t c a t g t a c t g g

SnaBI 360 CMV enhancer CMV promoter t t a t g g g a c t t t c c t a c t t g g c a g t a c a t c t a c g t a t t a g t c a t c g c t a t t a c c a t g g t g a a t a c c c t g a a a g g a t g a a c c g t c a t g t a g a t g c a t a a t c a g t a g c g a t a a t g g t a c c a c

420 CMV promoter a t g c g g t t t t g g c a g t a c a t c a a t g g g c g t g g a t a g c g g t t t g a c t c a c g g g g a t t t c c a t a c g c c a a a a c c g t c a t g t a g t t a c c c g c a c c t a t c g c c a a a c t g a g t g c c c c t a a a g g t

480 CMV promoter a g t c t c c a c c c c a t t g a c g t c a a t g g g a g t t t g t t t t g g c a c c a a a a t c a a c g g g a c t t t t c a g a g g t g g g g t a a c t g c a g t t a c c c t c a a a c a a a a c c g t g g t t t t a g t t g c c c t g a a a

540 CMV promoter C G C A A A T G G G C G G T A G G C G T G CMV-F c c a a a a t g t c g t a a c a a c t c c g c c c c a t t g a c g c a a a t g g g c g g t a g g c g t g t a c g g t g g g g t t t t a c a g c a t t g t t g a g g c g g g g t a a c t g c g t t t a c c c g c c a t c c g c a c a t g c c a c c

NheIBmtIAfeI 600 CMV promoter g a g g t c t a t a t a a g c a g a g c t g g t t t a g t g a a c c g t c a g a t c c g c t a g c g c t a c c g g a c t c t c c a g a t a t a t t c g t c t c g a c c a a a t c a c t t g g c a g t c t a g g c g a t c g c g a t g g c c t g a

XcmIPaeR7IXhoI 660 1 5 10 MKMDKKTIVWFRRD CRY2PHR MKMDKKTIVWFRRD c a g a t c t c g a g g c c a c c a t g a a g a t g g a c a a a a a g a c t a t a g t t t g g t t t a g a a g a g a c c g t c t a g a g c t c c g g t g g t a c t t c t a c c t g t t t t t c t g a t a t c a a a c c a a a t c t t c t c t g g

720 15 20 25 30 LRIEDNPALAAAAHEGSVFP CRY2PHR LRIEDNPALAAAAHEGSVFP t a a g g a t t g a g g a t a a t c c t g c a t t a g c a g c a g c t g c t c a c g a a g g a t c t g t t t t t c c t g a t t c c t a a c t c c t a t t a g g a c g t a a t c g t c g t c g a c g a g t g c t t c c t a g a c a a a a a g g a c

780 35 40 45 50 VFIWCPEEEGQFYPGRASRW CRY2PHR VFIWCPEEEGQFYPGRASRW t c t t c a t t t g g t g t c c t g a a g a a g a a g g a c a g t t t t a t c c t g g a a g a g c t t c a a g a t g g t a g a a g t a a a c c a c a g g a c t t c t t c t t c c t g t c a a a a t a g g a c c t t c t c g a a g t t c t a c c a

840 55 60 65 70 WMKQSLAHLSQSLKALGSDL CRY2PHR WMKQSLAHLSQSLKALGSDL g g a t g a a a c a a t c a c t t g c t c a c t t a t c t c a a t c c t t g a a g g c t c t t g g a t c t g a c c t c a c c t a c t t t g t t a g t g a a c g a g t g a a t a g a g t t a g g a a c t t c c g a g a a c c t a g a c t g g a g t

900 75 80 85 90 TLIKTHNTISAILDCIRVTG CRY2PHR TLIKTHNTISAILDCIRVTG c t t t a a t c a a a a c c c a c a a c a c g a t t t c a g c g a t c t t g g a t t g t a t c c g c g t t a c c g g t g g a a a t t a g t t t t g g g t g t t g t g c t a a a g t c g c t a g a a c c t a a c a t a g g c g c a a t g g c c a c

960 95 100 105 110 ATKVVFNHLYDPVSLVRDHT CRY2PHR ATKVVFNHLYDPVSLVRDHT c t a c a a a a g t c g t c t t t a a c c a c c t c t a t g a t c c t g t t t c g t t a g t t c g g g a c c a t a c c g g a t g t t t t c a g c a g a a a t t g g t g g a g a t a c t a g g a c a a a g c a a t c a a g c c c t g g t a t g g c

1020 115 120 125 130 VKEKLVERGISVQSYNGDLL CRY2PHR VKEKLVERGISVQSYNGDLL t a a a g g a g a a g c t g g t g g a a c g t g g g a t c t c t g t g c a a a g c t a c a a t g g a g a t c t a t t g t a t t t c c t c t t c g a c c a c c t t g c a c c c t a g a g a c a c g t t t c g a t g t t a c c t c t a g a t a a c a

1080 135 140 145 150 YEPWEIYCEKGKPFTSFNSY CRY2PHR YEPWEIYCEKGKPFTSFNSY a t g a a c c g t g g g a g a t a t a c t g c g a a a a g g g c a a a c c t t t t a c g a g t t t c a a t t c t t a c t t a c t t g g c a c c c t c t a t a t g a c g c t t t t c c c g t t t g g a a a a t g c t c a a a g t t a a g a a t g a

1140 155 160 165 170 WKKCLDMSIESVMLPPPWRL CRY2PHR WKKCLDMSIESVMLPPPWRL g g a a g a a a t g c t t a g a t a t g t c g a t t g a a t c c g t t a t g c t t c c t c c t c c t t g g c g g t t g a c c t t c t t t a c g a a t c t a t a c a g c t a a c t t a g g c a a t a c g a a g g a g g a g g a a c c g c c a a c t

1200 175 180 185 190 MPITAAAEAIWACSIEELGL CRY2PHR MPITAAAEAIWACSIEELGL t g c c a a t a a c t g c a g c g g c t g a a g c g a t t t g g g c g t g t t c g a t t g a a g a a c t a g g g c t g g a c g g t t a t t g a c g t c g c c g a c t t c g c t a a a c c c g c a c a a g c t a a c t t c t t g a t c c c g a c c

1260 195 200 205 210 ENEAEKPSNALLTRAWSPGW CRY2PHR ENEAEKPSNALLTRAWSPGW a g a a t g a g g c c g a g a a a c c g a g c a a t g c g t t g t t a a c t a g a g c t t g g t c t c c a g g a t g g a t c t t a c t c c g g c t c t t t g g c t c g t t a c g c a a c a a t t g a t c t c g a a c c a g a g g t c c t a c c t

1320 215 220 225 230 SNADKLLNEFIEKQLIDYAK CRY2PHR SNADKLLNEFIEKQLIDYAK g c a a t g c t g a t a a g t t a c t a a a t g a g t t c a t c g a g a a g c a g t t g a t a g a t t a t g c a a a g a c g t t a c g a c t a t t c a a t g a t t t a c t c a a g t a g c t c t t c g t c a a c t a t c t a a t a c g t t t c t

EcoRI 1380 235 240 245 250 NSKKVVGNSTSLLSPYLHFG CRY2PHR NSKKVVGNSTSLLSPYLHFG a c a g c a a g a a a g t t g t t g g g a a t t c t a c t t c a c t a c t t t c t c c g t a t c t c c a t t t c g g g g t g t c g t t c t t t c a a c a a c c c t t a a g a t g a a g t g a t g a a a g a g g c a t a g a g g t a a a g c c c c

1440 255 260 265 270 EISVRHVFQCARMKQIIWAR CRY2PHR EISVRHVFQCARMKQIIWAR a a a t a a g c g t c a g a c a c g t t t t c c a g t g t g c c c g g a t g a a a c a a a t t a t a t g g g c a a g a g t t t a t t c g c a g t c t g t g c a a a a g g t c a c a c g g g c c t a c t t t g t t t a a t a t a c c c g t t c t c

1500 275 280 285 290 DKNSEGEESADLFLRGIGLR CRY2PHR DKNSEGEESADLFLRGIGLR a t a a g a a c a g t g a a g g a g a a g a a a g t g c a g a t c t t t t t c t t a g g g g a a t c g g t t t a a g a g t a t t c t t g t c a c t t c c t c t t c t t t c a c g t c t a g a a a a a g a a t c c c c t t a g c c a a a t t c t c

1560 295 300 305 310 EYSRYICFNFPFTHEQSLLS CRY2PHR EYSRYICFNFPFTHEQSLLS a g t a t t c t c g g t a t a t a t g t t t c a a c t t c c c g t t t a c t c a c g a g c a a t c g t t g t t g a g t c t c a t a a g a g c c a t a t a t a c a a a g t t g a a g g g c a a a t g a g t g c t c g t t a g c a a c a a c t c a g

1620 315 320 325 330 HLRFFPWDADVDKFKAWRQG CRY2PHR HLRFFPWDADVDKFKAWRQG a t c t t c g g t t t t t c c c t t g g g a t g c t g a t g t t g a t a a g t t c a a g g c c t g g a g a c a a g g c a t a g a a g c c a a a a a g g g a a c c c t a c g a c t a c a a c t a t t c a a g t t c c g g a c c t c t g t t c c g t

1680 335 340 345 350 RTGYPLVDAGMRELWATGWM CRY2PHR RTGYPLVDAGMRELWATGWM g g a c c g g t t a t c c g t t g g t g g a t g c c g g a a t g a g a g a g c t t t g g g c t a c c g g a t g g a t g c c c t g g c c a a t a g g c a a c c a c c t a c g g c c t t a c t c t c t c g a a a c c c g a t g g c c t a c c t a c g

1740 355 360 365 370 HNRIRVIVSSFAVKFLLLPW CRY2PHR HNRIRVIVSSFAVKFLLLPW a t a a c a g a a t a a g a g t g a t t g t t t c a a g c t t t g c t g t g a a g t t t c t t c t c c t t c c a t g g a t a t t g t c t t a t t c t c a c t a a c a a a g t t c g a a a c g a c a c t t c a a a g a a g a g g a a g g t a c c t

1800 375 380 385 390 KWGMKYFWDTLLDADLECDI CRY2PHR KWGMKYFWDTLLDADLECDI a a t g g g g a a t g a a g t a t t t c t g g g a t a c a c t t t t g g a t g c t g a t t t g g a a t g t g a c a t c c t t a c c c c t t a c t t c a t a a a g a c c c t a t g t g a a a a c c t a c g a c t a a a c c t t a c a c t g t a g g

1860 395 400 405 410 LGWQYISGSIPDGHELDRLD CRY2PHR LGWQYISGSIPDGHELDRLD t t g g c t g g c a g t a t a t c t c t g g g a g t a t c c c c g a t g g c c a c g a g c t t g a t c g c t t g g a c a a a c c g a c c g t c a t a t a g a g a c c c t c a t a g g g g c t a c c g g t g c t c g a a c t a g c g a a c c t g t

1920 415 420 425 430 NPALQGAKYDPEGEYIRQWL CRY2PHR NPALQGAKYDPEGEYIRQWL a t c c c g c g t t a c a a g g c g c c a a a t a t g a c c c a g a a g g t g a g t a c a t a a g g c a a t g g c t t c t a g g g c g c a a t g t t c c g c g g t t t a t a c t g g g t c t t c c a c t c a t g t a t t c c g t t a c c g a a g

1980 435 440 445 450 PELARLPTEWIHHPWDAPLT CRY2PHR PELARLPTEWIHHPWDAPLT c c g a g c t t g c g a g a t t g c c a a c t g a a t g g a t c c a t c a t c c a t g g g a c g c t c c t t t a a c c g g g c t c g a a c g c t c t a a c g g t t g a c t t a c c t a g g t a g t a g g t a c c c t g c g a g g a a a t t g g c

2040 455 460 465 470 VLKASGVELGTNYAKPIVDI CRY2PHR VLKASGVELGTNYAKPIVDI t a c t c a a a g c t t c t g g t g t g g a a c t c g g a a c a a a c t a t g c g a a a c c c a t t g t a g a c a t c g a t g a g t t t c g a a g a c c a c a c c t t g a g c c t t g t t t g a t a c g c t t t g g g t a a c a t c t g t a g c

2100 475 480 485 490 DTARELLAKAISRTREAQIM CRY2PHR DTARELLAKAISRTREAQIM a c a c a g c t c g t g a g c t a c t a g c t a a a g c t a t t t c a a g a a c c c g t g a a g c a c a g a t c a t g a t g t g t c g a g c a c t c g a t g a t c g a t t t c g a t a a a g t t c t t g g g c a c t t c g t g t c t a g t a c t

2160 495 IGAA ARV 1 M 1a V 5 10 SKGEELFTGVV CRY2PHR EYFP IGAAARVMVSKGEELFTGVV t c g g a g c a g c a g c c c g g g t c a t g g t g a g c a a g g g c g a g g a g c t g t t c a c c g g g g t g g t g c a g c c t c g t c g t c g g g c c c a g t a c c a c t c g t t c c c g c t c c t c g a c a a g t g g c c c c a c c a c g

2220 15 20 25 30 PILVELDGDVNGHKFSVSGE EYFP G A C C A G C T C G A C C T G C C G C T G C EGFP-N PILVELDGDVNGHKFSVSGE c c a t c c t g g t c g a g c t g g a c g g c g a c g t a a a c g g c c a c a a g t t c a g c g t g t c c g g c g a g g g g t a g g a c c a g c t c g a c c t g c c g c t g c a t t t g c c g g t g t t c a a g t c g c a c a g g c c g c t c c

2280 35 40 45 50 GEGDATYGKLTLKFICTTGK EYFP GEGDATYGKLTLKFICTTGK g c g a g g g c g a t g c c a c c t a c g g c a a g c t g a c c c t g a a g t t c a t c t g c a c c a c c g g c a a g c c g c t c c c g c t a c g g t g g a t g c c g t t c g a c t g g g a c t t c a a g t a g a c g t g g t g g c c g t t c g

2340 55 60 65 70 LPVPWPTLVTTFGYGLQCFA EYFP LPVPWPTLVTTFGYGLQCFA t g c c c g t g c c c t g g c c c a c c c t c g t g a c c a c c t t c g g c t a c g g c c t g c a g t g c t t c g c c c a c g g g c a c g g g a c c g g g t g g g a g c a c t g g t g g a a g c c g a t g c c g g a c g t c a c g a a g c g g g

2400 75 80 85 90 RYPDHMKQHDFFKSAMPEGY EYFP RYPDHMKQHDFFKSAMPEGY g c t a c c c c g a c c a c a t g a a g c a g c a c g a c t t c t t c a a g t c c g c c a t g c c c g a a g g c t a c g c g a t g g g g c t g g t g t a c t t c g t c g t g c t g a a g a a g t t c a g g c g g t a c g g g c t t c c g a t g c

2460 95 100 105 110 VQERTIFFKDDGNYKTRAEV EYFP C T G C T G C C G T T G A T G T T C T G EXFP-R VQERTIFFKDDGNYKTRAEV t c c a g g a g c g c a c c a t c t t c t t c a a g g a c g a c g g c a a c t a c a a g a c c c g c g c c g a g g t g a a g g t c c t c g c g t g g t a g a a g a a g t t c c t g c t g c c g t t g a t g t t c t g g g c g c g g c t c c a c t

2520 115 120 125 130 KFEGDTLVNRIELKGIDFKE EYFP KFEGDTLVNRIELKGIDFKE a g t t c g a g g g c g a c a c c c t g g t g a a c c g c a t c g a g c t g a a g g g c a t c g a c t t c a a g g a g g t c a a g c t c c c g c t g t g g g a c c a c t t g g c g t a g c t c g a c t t c c c g t a g c t g a a g t t c c t c c

2580 135 140 145 150 DGNILGHKLEYNYNSHNVYI EYFP DGNILGHKLEYNYNSHNVYI a c g g c a a c a t c c t g g g g c a c a a g c t g g a g t a c a a c t a c a a c a g c c a c a a c g t c t a t a t c a t g c c g t t g t a g g a c c c c g t g t t c g a c c t c a t g t t g a t g t t g t c g g t g t t g c a g a t a t a g t

2640 155 160 165 170 MADKQKNGIKVNFKIRHNIE EYFP MADKQKNGIKVNFKIRHNIE t g g c c g a c a a g c a g a a g a a c g g c a t c a a g g t g a a c t t c a a g a t c c g c c a c a a c a t c g a g g a c c g g c t g t t c g t c t t c t t g c c g t a g t t c c a c t t g a a g t t c t a g g c g g t g t t g t a g c t c c

2700 175 180 185 190 DGSVQLADHYQQNTPIGDGP EYFP DGSVQLADHYQQNTPIGDGP a c g g c a g c g t g c a g c t c g c c g a c c a c t a c c a g c a g a a c a c c c c c a t c g g c g a c g g c c c c g t g c c g t c g c a c g t c g a g c g g c t g g t g a t g g t c g t c t t g t g g g g g t a g c c g c t g c c g g g g c

2760 195 200 205 210 VLLPDNHYLSYQSALSKDPN EYFP VLLPDNHYLSYQSALSKDPN t g c t g c t g c c c g a c a a c c a c t a c c t g a g c t a c c a g t c c g c c c t g a g c a a a g a c c c c a a c g a c g a c g a c g g g c t g t t g g t g a t g g a c t c g a t g g t c a g g c g g g a c t c g t t t c t g g g g t t g c

2820 215 220 225 230 EKRDHMVLLEFVTAAGITLG EYFP C A T G G T C C T G C T G G A G T T C G T G EGFP-C EKRDHMVLLEFVTAAGITLG a g a a g c g c g a t c a c a t g g t c c t g c t g g a g t t c g t g a c c g c c g c c g g g a t c a c t c t c g g c a t c t t c g c g c t a g t g t a c c a g g a c g a c c t c a a g c a c t g g c g g c g g c c c t a g t g a g a g c c g t

NotIBsrGI 2880 235 MDELYK (in frame with EYFP) SGRMAEPSQAPTPA EYFP MDELYKSGRMAEPSQAPTPA t g g a c g a g c t g t a c a a g a g c g g c c g c a t g g c g g a a c c t t c c c a g g c c c c g a c c c c g g c c c a c c t g c t c g a c a t g t t c t c g c c g g c g t a c c g c c t t g g a a g g g t c c g g g g c t g g g g c c g g g

2940 (in frame with EYFP) PAAQPRPLQSPAPAPTPTPA PAAQPRPLQSPAPAPTPTPA c g g c t g c g c a g c c c c g g c c c c t t c a g t c c c c a g c c c c t g c c c c a a c t c c g a c t c c t g c a c g c c g a c g c g t c g g g g c c g g g g a a g t c a g g g g t c g g g g a c g g g g t t g a g g c t g a g g a c g t g

3000 (in frame with EYFP) PSPASAPIPTPTPAPAPAPA PSPASAPIPTPTPAPAPAPA c c a g c c c g g c t t c a g c c c c g a t t c c g a c t c c c a c c c c g g c a c c a g c c c c t g c c c c a g c t g g g t c g g g c c g a a g t c g g g g c t a a g g c t g a g g g t g g g g c c g t g g t c g g g g a c g g g g t c g a c

PspOMIApaI 3060 (in frame with EYFP) AAPAGSTGTGGPGVGSGGAG AAPAGSTGTGGPGVGSGGAG c a g c c c c a g c c g g c a g c a c a g g g a c t g g g g g g c c c g g g g t a g g a a g t g g g g g g g c c g g g a g t c g g g g t c g g c c g t c g t g t c c c t g a c c c c c c g g g c c c c a t c c t t c a c c c c c c c g g c c c t

3120 (in frame with EYFP) SGGDPARPGLSQQQRASQRK SGGDPARPGLSQQQRASQRK g c g g g g g g g a t c c g g c t c g a c c t g g c c t g a g c c a g c a g c a g c g c g c c a g t c a g a g g a a g g c g c c c c c c c t a g g c c g a g c t g g a c c g g a c t c g g t c g t c g t c g c g c g g t c a g t c t c c t t c c

3180 (in frame with EYFP) AQVRGLPRAKKLEKLGVFSA AQVRGLPRAKKLEKLGVFSA c g c a a g t c c g g g g g c t g c c g c g c g c c a a g a a g c t t g a g a a g c t a g g g g t c t t c t c g g c t t g c g t t c a g g c c c c c g a c g g c g c g c g g t t c t t c g a a c t c t t c g a t c c c c a g a a g a g c c g a a

3240 (in frame with EYFP) CKANETCKCNGWKNPKPPTA CKANETCKCNGWKNPKPPTA g c a a g g c c a a t g a a a c c t g t a a g t g t a a t g g c t g g a a a a a c c c c a a g c c c c c c a c t g c a c c g t t c c g g t t a c t t t g g a c a t t c a c a t t a c c g a c c t t t t t g g g g t t c g g g g g g t g a c g t g

3300 (in frame with EYFP) PRMDLQQPAANLSELCRSCE PRMDLQQPAANLSELCRSCE c c c g c a t g g a t c t g c a g c a g c c a g c t g c c a a c c t g a g t g a g c t g t g c c g c a g t t g t g a g c g g g c g t a c c t a g a c g t c g t c g g t c g a c g g t t g g a c t c a c t c g a c a c g g c g t c a a c a c t c g

3360 (in frame with EYFP) HPLADHVSHLENVSEDEINR HPLADHVSHLENVSEDEINR a c c c c t t g g c t g a c c a c g t a t c c c a c t t g g a g a a t g t g t c a g a g g a t g a g a t a a a c c g a c t g g g g a a c c g a c t g g t g c a t a g g g t g a a c c t c t t a c a c a g t c t c c t a c t c t a t t t g g c t g

3420 (in frame with EYFP) LLGMVVDVENLFMSVHKGED LLGMVVDVENLFMSVHKGED t g c t g g g g a t g g t g g t g g a t g t g g a g a a t c t c t t c a t g t c t g t t c a c a a g g g a g a g g a c a a c g a c c c c t a c c a c c a c c t a c a c c t c t t a g a g a a g t a c a g a c a a g t g t t c c c t c t c c t g t

3480 (in frame with EYFP) TDTNQVYFYLFKLLRKCILQ TDTNQVYFYLFKLLRKCILQ c a g a c a c c a a t c a g g t c t a t t t c t a c c t c t t c a a g c t a c t g c g g a a a t g c a t c c t g c a g a g t c t g t g g t t a g t c c a g a t a a a g a t g g a g a a g t t c g a t g a c g c c t t t a c g t a g g a c g t c t

3540 (in frame with EYFP) MTRPVVEGSLGSPPFEKPNI MTRPVVEGSLGSPPFEKPNI t g a c c c g g c c t g t g g t g g a g g g g t c c c t g g g c a g c c c t c c a t t t g a g a a a c c t a a t a t t g a c t g g g c c g g a c a c c a c c t c c c c a g g g a c c c g t c g g g a g g t a a a c t c t t t g g a t t a t a a c

3600 (in frame with EYFP) EQGVLNFVQYKFSHLAPRER EQGVLNFVQYKFSHLAPRER a g c a g g g t g t g c t g a a c t t t g t g c a g t a c a a a t t t a g t c a c c t g g c t c c c c g g g a g c g g c t c g t c c c a c a c g a c t t g a a a c a c g t c a t g t t t a a a t c a g t g g a c c g a g g g g c c c t c g c c g

Eco53kISacI 3660 (in frame with EYFP) QTMFELSKMFLLCLNYWKLE QTMFELSKMFLLCLNYWKLE a g a c g a t g t t c g a g c t c t c a a a g a t g t t c t t g c t c t g c c t t a a c t a c t g g a a g c t t g a g a t c t g c t a c a a g c t c g a g a g t t t c t a c a a g a a c g a g a c g g a a t t g a t g a c c t t c g a a c t c t

BmgBIBbvCIPaqCI 3720 (in frame with EYFP) TPAQFRQRSQAEDVATYKVN TPAQFRQRSQAEDVATYKVN c a c c t g c c c a g t t t c g g c a g a g g t c t c a g g c t g a g g a c g t g g c t a c c t a c a a g g t c a a t t g t g g a c g g g t c a a a g c c g t c t c c a g a g t c c g a c t c c t g c a c c g a t g g a t g t t c c a g t t a a

PmlI 3780 (in frame with EYFP) YTRWLCYCHVPQSCDSLPRY YTRWLCYCHVPQSCDSLPRY a c a c c a g a t g g c t c t g t t a c t g c c a c g t g c c c c a g a g c t g t g a t a g c c t c c c c c g c t a c g t g t g g t c t a c c g a g a c a a t g a c g g t g c a c g g g g t c t c g a c a c t a t c g g a g g g g g c g a t g c

3840 (in frame with EYFP) ETTHVFGRSLLRSIFTVTRR ETTHVFGRSLLRSIFTVTRR a a a c c a c t c a t g t c t t t g g g c g a a g c c t t c t c c g g t c c a t t t t c a c c g t t a c c c g c c g g c t t t g g t g a g t a c a g a a a c c c g c t t c g g a a g a g g c c a g g t a a a a g t g g c a a t g g g c g g c c g

3900 (in frame with EYFP) QLLEKFRVEKDKLVPEKRTL QLLEKFRVEKDKLVPEKRTL a g c t g c t g g a a a a g t t c c g a g t g g a g a a g g a c a a a t t g g t g c c c g a g a a g a g g a c c c t c a t c g a c g a c c t t t t c a a g g c t c a c c t c t t c c t g t t t a a c c a c g g g c t c t t c t c c t g g g a g t

3960 (in frame with EYFP) ILTHFPKFLSMLEEEIYGAN ILTHFPKFLSMLEEEIYGAN *PAFE t c c t c a c t c a c t t c c c c a a a t t c c t g t c c a t g c t g g a g g a g g a g a t c t a t g g g g c a a a c t a g g a g t g a g t g a a g g g g t t t a a g g a c a g g t a c g a c c t c c t c c t c t a g a t a c c c c g t t t g a

4020 (in frame with EYFP) SPIWESGFTMPPSEGTQLVP SPIWESGFTMPPSEGTQLVP GIQSDPKVMGGESPVCSTGR c t c c a a t c t g g g a g t c a g g c t t c a c c a t g c c a c c c t c a g a g g g g a c a c a g c t g g t t c c c c g a g g t t a g a c c c t c a g t c c g a a g t g g t a c g g t g g g a g t c t c c c c t g t g t c g a c c a a g g g g

4080 (in frame with EYFP) RPASVSAAVVPSTPIFSPSM RPASVSAAVVPSTPIFSPSM GAETLAATTGLVGMKLGLMP g g c c a g c t t c a g t c a g t g c a g c g g t t g t t c c c a g c a c c c c c a t c t t c a g c c c c a g c a t g g c c g g t c g a a g t c a g t c a c g t c g c c a a c a a g g g t c g t g g g g g t a g a a g t c g g g g t c g t a c c

EcoNI 4140 (in frame with EYFP) GGGSNSSLSLDSAGAEPMPG GGGSNSSLSLDSAGAEPMPG PPLLLERLRSEAPASGIGPS g t g g g g g c a g c a a c a g c t c c c t g a g t c t g g a t t c t g c a g g g g c c g a g c c t a t g c c a g g c g c a c c c c c g t c g t t g t c g a g g g a c t c a g a c c t a a g a c g t c c c c g g c t c g g a t a c g g t c c g c

4200 (in frame with EYFP) EKRTLPENLTLEDAKRLRVM EKRTLPENLTLEDAKRLRVM FLVSGSFRVRSSALRSRTIP a g a a g a g g a c g c t c c c a g a g a a c c t g a c c c t g g a g g a t g c c a a g c g g c t c c g t g t g a t g g t c t t c t c c t g c g a g g g t c t c t t g g a c t g g g a c c t c c t a c g g t t c g c c g a g g c a c a c t a c c

4260 (in frame with EYFP) GDIPMELVNEVMLTITDPAA GDIPMELVNEVMLTITDPAA SMGMSSTLSTMSVMVSGAAM g t g a c a t c c c c a t g g a g c t g g t c a a t g a g g t c a t g c t g a c c a t c a c t g a c c c t g c t g c c a c a c t g t a g g g g t a c c t c g a c c a g t t a c t c c a g t a c g a c t g g t a g t g a c t g g g a c g a c g g t

BsmBIEsp3I 4320 (in frame with EYFP) MLGPETSLLSANAARDETAR MLGPETSLLSANAARDETAR SPGSVLRSEALAARSSVARR t g c t g g g g c c t g a g a c g a g c c t g c t t t c g g c c a a t g c g g c c c g g g a t g a g a c a g c c c g c c a c g a c c c c g g a c t c t g c t c g g a c g a a a g c c g g t t a c g c c g g g c c c t a c t c t g t c g g g c g g

SacII 4380 (in frame with EYFP) LEERRGIIEFHVIGNSLTPK LEERRGIIEFHVIGNSLTPK SSRRPMMSNWTMPLESVGLA t g g a g g a g c g c c g c g g c a t c a t c g a g t t c c a t g t c a t c g g c a a c t c a c t g a c g c c c a a g g a c c t c c t c g c g g c g c c g t a g t a g c t c a a g g t a c a g t a g c c g t t g a g t g a c t g c g g g t t c c

4440 (in frame with EYFP) ANRRVLLWLVGLQNVFSHQL ANRRVLLWLVGLQNVFSHQL LRRTNSHSTPSCFTKEWWSG c c a a c c g g c g g g t g t t g c t g t g g c t c g t g g g g c t g c a g a a t g t c t t t t c c c a c c a g c t g c g g t t g g c c g c c c a c a a c g a c a c c g a g c a c c c c g a c g t c t t a c a g a a a a g g g t g g t c g a c g

4500 (in frame with EYFP) PRMPKEYIARLVFDPKHKTL PRMPKEYIARLVFDPKHKTL RMGLSYIARRTKSGFCLVRA c g c g c a t g c c t a a g g a g t a t a t c g c c c g c c t c g t c t t t g a c c c g a a g c a c a a g a c t c t g g g c g c g t a c g g a t t c c t c a t a t a g c g g g c g g a g c a g a a a c t g g g c t t c g t g t t c t g a g a c c

BclI* 4560 (in frame with EYFP) ALIKDGRVIGGICFRMFPTQ ALIKDGRVIGGICFRMFPTQ KILSPRTMPPMQKRM c c t t g a t c a a g g a t g g g c g g g t c a t c g g t g g c a t c t g c t t c c g c a t g t t t c c c a c c c a g g g g a a c t a g t t c c t a c c c g c c c a g t a g c c a c c g t a g a c g a a g g c g t a c a a a g g g t g g g t c c

4620 (in frame with EYFP) GFTEIVFCAVTSNEQVKGYG GFTEIVFCAVTSNEQVKGYG g c t t c a c g g a g a t t g t c t t c t g t g c t g t c a c c t c g a a t g a g c a g g t c a a g g g t t a t g g g a c g a a g t g c c t c t a a c a g a a g a c a c g a c a g t g g a g c t t a c t c g t c c a g t t c c c a a t a c c c t

4680 (in frame with EYFP) THLMNHLKEYHIKHNILYFL THLMNHLKEYHIKHNILYFL c c c a c c t g a t g a a c c a c c t g a a g g a g t a t c a c a t c a a g c a c a a c a t t c t c t a c t t c c t c a g g g t g g a c t a c t t g g t g g a c t t c c t c a t a g t g t a g t t c g t g t t g t a a g a g a t g a a g g a g t

4740 (in frame with EYFP) TYADEYAIGYFKKQGFSKDI TYADEYAIGYFKKQGFSKDI c c t a c g c c g a c g a g t a c g c c a t c g g c t a c t t c a a a a a g c a g g g t t t c t c c a a g g a c a t c a g g a t g c g g c t g c t c a t g c g g t a g c c g a t g a a g t t t t t c g t c c c a a a g a g g t t c c t g t a g t

4800 (in frame with EYFP) KVPKSRYLGYIKDYEGATLM KVPKSRYLGYIKDYEGATLM a g g t g c c c a a g a g c c g c t a c c t g g g c t a c a t c a a g g a c t a c g a g g g a g c g a c g c t g a t g g t c c a c g g g t t c t c g g c g a t g g a c c c g a t g t a g t t c c t g a t g c t c c c t c g c t g c g a c t a c c

4860 (in frame with EYFP) ECELNPRIPYTELSHIIKKQ ECELNPRIPYTELSHIIKKQ a g t g t g a g c t g a a t c c c c g c a t c c c c t a c a c g g a g c t g t c c c a c a t c a t c a a g a a g c a g a t c a c a c t c g a c t t a g g g g c g t a g g g g a t g t g c c t c g a c a g g g t g t a g t a g t t c t t c g t c t

4920 (in frame with EYFP) KEIIKKLIERKQAQIRKVYP KEIIKKLIERKQAQIRKVYP a a g a g a t c a t c a a g a a g c t g a t t g a g c g c a a a c a g g c c c a g a t c c g c a a g g t c t a c c c g g t t c t c t a g t a g t t c t t c g a c t a a c t c g c g t t t g t c c g g g t c t a g g c g t t c c a g a t g g g c c

BlpI 4980 (in frame with EYFP) GLSCFKEGVRQIPVESVPGI GLSCFKEGVRQIPVESVPGI g g c t c a g c t g c t t c a a g g a g g g c g t g a g g c a g a t c c c t g t g g a g a g c g t t c c t g g c a t t c c c g a g t c g a c g a a g t t c c t c c c g c a c t c c g t c t a g g g a c a c c t c t c g c a a g g a c c g t a a g

5040 (in frame with EYFP) RETGWKPLGKEKGKELKDPD RETGWKPLGKEKGKELKDPD g a g a g a c a g g c t g g a a g c c a t t g g g g a a g g a g a a g g g g a a g g a g c t g a a g g a c c c c g a c c c t c t c t g t c c g a c c t t c g g t a a c c c c t t c c t c t t c c c c t t c c t c g a c t t c c t g g g g c t g g

PflMI* 5100 (in frame with EYFP) QLYTTLKNLLAQIKSHPSAW QLYTTLKNLLAQIKSHPSAW a g c t c t a c a c a a c c c t c a a a a a c c t g c t g g c c c a a a t c a a g t c t c a c c c c a g t g c c t g g c t c g a g a t g t g t t g g g a g t t t t t g g a c g a c c g g g t t t a g t t c a g a g t g g g g t c a c g g a c c g

5160 (in frame with EYFP) PFMEPVKKSEAPDYYEVIRF PFMEPVKKSEAPDYYEVIRF c c t t c a t g g a g c c t g t g a a g a a g t c g g a g g c c c c t g a c t a c t a c g a g g t c a t c c g c t t c c g g a a g t a c c t c g g a c a c t t c t t c a g c c t c c g g g g a c t g a t g a t g c t c c a g t a g g c g a a g g

5220 (in frame with EYFP) PIDLKTMTERLRSRYYVTRK PIDLKTMTERLRSRYYVTRK c c a t t g a c c t g a a g a c c a t g a c t g a g c g g c t g c g a a g c c g c t a c t a c g t g a c c c g g a a g c g g t a a c t g g a c t t c t g g t a c t g a c t c g c c g a c g c t t c g g c g a t g a t g c a c t g g g c c t t c g

NruI 5280 (in frame with EYFP) LFVADLQRVIANCREYNPPD LFVADLQRVIANCREYNPPD t c t t t g t g g c c g a c c t g c a g c g g g t c a t c g c c a a c t g t c g c g a g t a c a a c c c c c c g g a c a a g a a a c a c c g g c t g g a c g t c g c c c a g t a g c g g t t g a c a g c g c t c a t g t t g g g g g g c c t g t

ScaI 5340 (in frame with EYFP) SEYCRCASALEKFFYFKLKE SEYCRCASALEKFFYFKLKE g c g a g t a c t g c c g c t g t g c c a g c g c c c t g g a g a a g t t c t t c t a c t t c a a g c t c a a g g a g g c g c t c a t g a c g g c g a c a c g g t c g c g g g a c c t c t t c a a g a a g a t g a a g t t c g a g t t c c t c c

XbaI*AhdI 5400 (in frame with EYFP) GGLIDK* GGLIDK* g a g g c c t c a t t g a c a a g t a g t c t a g a t c a t a a t c a g c c a t a c c a c a t t t g t a g a g g t t t t c t c c g g a g t a a c t g t t c a t c a g a t c t a g t a t t a g t c g g t a t g g t g t a a a c a t c t c c a a a a

MfeI 5460 a c t t g c t t t a a a a a a c c t c c c a c a c c t c c c c c t g a a c c t g a a a c a t a a a a t g a a t g c a a t t g a a c g a a a t t t t t t g g a g g g t g t g g a g g g g g a c t t g g a c t t t g t a t t t t a c t t a c g t t a

5520 SV40 poly(A) signal C G T T A T C G T A G T G SV40pA-R t g t t g t t g t t a a c t t g t t t a t t g c a g c t t a t a a t g g t t a c a a a t a a a g c a a t a g c a t c a c a c a a c a a c a a t t g a a c a a a t a a c g t c g a a t a t t a c c a a t g t t t a t t t c g t t a t c g t a g t g

5580 SV40 poly(A) signal G T G G T T T G T C C A A A C T C A T EBV-rev T T T A A A G SV40pA-R a a a t t t c a c a a a t a a a g c a t t t t t t t c a c t g c a t t c t a g t t g t g g t t t g t c c a a a c t c a t t t t a a a g t g t t t a t t t c g t a a a a a a a g t g a c g t a a g a t c a a c a c c a a a c a g g t t t g a g t a

AflII 5640 SV40 poly(A) signal f1 ori C EBV-rev c a a t g t a t c t t a a g g c g t a a a t t g t a a g c g t t a a t a t t t t g t t a a a a t t c g c g t t a a a t t g t t a c a t a g a a t t c c g c a t t t a a c a t t c g c a a t t a t a a a a c a a t t t t a a g c g c a a t t t a a

5700 f1 ori t t t g t t a a a t c a g c t c a t t t t t t a a c c a a t a g g c c g a a a t c g g c a a a a t c c c t t a t a a a t a a a c a a t t t a g t c g a g t a a a a a a t t g g t t a t c c g g c t t t a g c c g t t t t a g g g a a t a t t t a

5760 f1 ori G G T C A A A C C T T G T T C T C A G G T G F1ori-F c a a a a g a a t a g a c c g a g a t a g g g t t g a g t g t t g t t c c a g t t t g g a a c a a g a g t c c a c t a t g t t t t c t t a t c t g g c t c t a t c c c a a c t c a c a a c a a g g t c a a a c c t t g t t c t c a g g t g a t a

5820 f1 ori t a a a g a a c g t g g a c t c c a a c g t c a a a g g g c g a a a a a c c g t c t a t c a g g g c g a t g g c c c a c a t t t c t t g c a c c t g a g g t t g c a g t t t c c c g c t t t t t g g c a g a t a g t c c c g c t a c c g g g t g

DraIII 5880 f1 ori t a c g t g a a c c a t c a c c c t a a t c a a g t t t t t t g g g g t c g a g g t g c c g t a a a g c a c t a a a t c a t g c a c t t g g t a g t g g g a t t a g t t c a a a a a a c c c c a g c t c c a c g g c a t t t c g t g a t t t a g

5940 f1 ori g g a a c c c t a a a g g g a g c c c c c g a t t t a g a g c t t g a c g g g g a a a g c c g g c g a a c g t g g c g a c c t t g g g a t t t c c c t c g g g g g c t a a a t c t c g a a c t g c c c c t t t c g g c c g c t t g c a c c g c t

6000 f1 ori A G G G A A G A A A G C G A A A G G A G F1ori-R g a a a g g a a g g g a a g a a a g c g a a a g g a g c g g g c g c t a g g g c g c t g g c a a g t g t a g c g g t c a c t t t c c t t c c c t t c t t t c g c t t t c c t c g c c c g c g a t c c c g c g a c c g t t c a c a t c g c c a g t

6060 f1 ori c g c t g c g c g t a a c c a c c a c a c c c g c c g c g c t t a a t g c g c c g c t a c a g g g c g c g t c a g g t g g c g a c g c g c a t t g g t g g t g t g g g c g g c g c g a a t t a c g c g g c g a t g t c c c g c g c a g t c c a c

6120 AmpR promoter g c a c t t t t c g g g g a a a t g t g c g c g g a a c c c c t a t t t g t t t a t t t t t c t a a a t a c a t t c a a c g t g a a a a g c c c c t t t a c a c g c g c c t t g g g g a t a a a c a a a t a a a a a g a t t t a t g t a a g t t

6180 AmpR promoter a t a t g t a t c c g c t c a t g a g a c a a t a a c c c t g a t a a a t g c t t c a a t a a t a t t g a a a a a g g a t a t a c a t a g g c g a g t a c t c t g t t a t t g g g a c t a t t t a c g a a g t t a t t a t a a c t t t t t c c t

6240 AmpR promoter SV40 promoter SV40 enhancer C C T T A C A C A C A G T C A A T C C C A pBABE 3' a g a g t c c t g a g g c g g a a a g a a c c a g c t g t g g a a t g t g t g t c a g t t a g g g t g t g g a a a g t c t c t c a g g a c t c c g c c t t t c t t g g t c g a c a c c t t a c a c a c a g t c a a t c c c a c a c c t t t c a g

SexAI*CsiI 6300 SV40 promoter SV40 enhancer c c c a g g c t c c c c a g c a g g c a g a a g t a t g c a a a g c a t g c a t c t c a a t t a g t c a g c a a c c a g g g g t c c g a g g g g t c g t c c g t c t t c a t a c g t t t c g t a c g t a g a g t t a a t c a g t c g t t g g t c

6360 SV40 promoter SV40 enhancer g t g t g g a a a g t c c c c a g g c t c c c c a g c a g g c a g a a g t a t g c a a a g c a t g c a t c t c a a t t a c a c a c c t t t c a g g g g t c c g a g g g g t c g t c c g t c t t c a t a c g t t t c g t a c g t a g a g t t a a t

6420 SV40 promoter SV40 enhancer SV40 ori g t c a g c a a c c a t a g t c c c g c c c c t a a c t c c g c c c a t c c c g c c c c t a a c t c c g c c c a g t t c c a g t c g t t g g t a t c a g g g c g g g g a t t g a g g c g g g t a g g g c g g g g a t t g a g g c g g g t c a a g

6480 SV40 promoter SV40 ori c g c c c a t t c t c c g c c c c a t g g c t g a c t a a t t t t t t t t a t t t a t g c a g a g g c c g a g g c c g c g c g g g t a a g a g g c g g g g t a c c g a c t g a t t a a a a a a a a t a a a t a c g t c t c c g g c t c c g g c g

6540 SV40 promoter SV40 ori c t c g g c c t c t g a g c t a t t c c a g a a g t a g t g a g g a g g c t t t t t t g g a g g c c t a g g c t t t t g g a g c c g g a g a c t c g a t a a g g t c t t c a t c a c t c c t c c g a a a a a a c c t c c g g a t c c g a a a a c

BspDI*ClaI* 6600 1 5 MIEQDGL SV40 promoter NeoR/KanR 1 5 MIEQDGL NeoR/KanR MIEQDGL c a a a g a t c g a t c a a g a g a c a g g a t g a g g a t c g t t t c g c a t g a t t g a a c a a g a t g g a t t g c g t t t c t a g c t a g t t c t c t g t c c t a c t c c t a g c a a a g c g t a c t a a c t t g t t c t a c c t a a c g

6660 10 15 20 25 HAGSPAAWVERLFGYDWAQQ NeoR/KanR 10 15 20 25 HAGSPAAWVERLFGYDWAQQ NeoR/KanR G A T A A G C C G A T A C T G A C C C G Neo-R HAGSPAAWVERLFGYDWAQQ a c g c a g g t t c t c c g g c c g c t t g g g t g g a g a g g c t a t t c g g c t a t g a c t g g g c a c a a c a g a t g c g t c c a a g a g g c c g g c g a a c c c a c c t c t c c g a t a a g c c g a t a c t g a c c c g t g t t g t c t

6720 30 35 40 45 TIGCSDAAVFRLSAQGRPVL NeoR/KanR 30 35 40 45 TIGCSDAAVFRLSAQGRPVL NeoR/KanR TIGCSDAAVFRLSAQGRPVL c a a t c g g c t g c t c t g a t g c c g c c g t g t t c c g g c t g t c a g c g c a g g g g c g c c c g g t t c t t t g t t a g c c g a c g a g a c t a c g g c g g c a c a a g g c c g a c a g t c g c g t c c c c g c g g g c c a a g a a a

6780 50 55 60 65 FVKTDLSGALNELQDEAARL NeoR/KanR 50 55 60 65 FVKTDLSGALNELQDEAARL NeoR/KanR FVKTDLSGALNELQDEAARL t t g t c a a g a c c g a c c t g t c c g g t g c c c t g a a t g a a c t g c a a g a c g a g g c a g c g c g g c t a t a a c a g t t c t g g c t g g a c a g g c c a c g g g a c t t a c t t g a c g t t c t g c t c c g t c g c g c c g a t a

Tth111IPflFI 6840 70 75 80 85 SWLATTGVPCAAVLDVVTEA NeoR/KanR 70 75 80 85 SWLATTGVPCAAVLDVVTEA NeoR/KanR SWLATTGVPCAAVLDVVTEA c g t g g c t g g c c a c g a c g g g c g t t c c t t g c g c a g c t g t g c t c g a c g t t g t c a c t g a a g c g g g c a c c g a c c g g t g c t g c c c g c a a g g a a c g c g t c g a c a c g a g c t g c a a c a g t g a c t t c g c c

6900 90 95 100 105 GRDWLLLGEVPGQDLLSSHL NeoR/KanR 90 95 100 105 GRDWLLLGEVPGQDLLSSHL NeoR/KanR GRDWLLLGEVPGQDLLSSHL *RVK g a a g g g a c t g g c t g c t a t t g g g c g a a g t g c c g g g g c a g g a t c t c c t g t c a t c t c a c c t t g c t t c c c t g a c c g a c g a t a a c c c g c t t c a c g g c c c c g t c c t a g a g g a c a g t a g a g t g g a a c

6960 110 115 120 125 APAEKVSIMADAMRRLHTLD NeoR/KanR 110 115 120 125 APAEKVSIMADAMRRLHTLD NeoR/KanR APAEKVSIMADAMRRLHTLD SRGLFYGDHSICHPPQMRKI c t c c t g c c g a g a a a g t a t c c a t c a t g g c t g a t g c a a t g c g g c g g c t g c a t a c g c t t g a t c g a g g a c g g c t c t t t c a t a g g t a g t a c c g a c t a c g t t a c g c c g c c g a c g t a t g c g a a c t a g

7020 130 135 140 145 PATCPFDHQAKHRIERARTR NeoR/KanR 130 135 140 145 PATCPFDHQAKHRIERARTR NeoR/KanR PATCPFDHQAKHRIERARTR RSGAWEVVLRFMADLSCTSP c g g c t a c c t g c c c a t t c g a c c a c c a a g c g a a a c a t c g c a t c g a g c g a g c a c g t a c t c g g a g c c g a t g g a c g g g t a a g c t g g t g g t t c g c t t t g t a g c g t a g c t c g c t c g t g c a t g a g c c t

7080 150 155 160 165 MEAGLVDQDDLDEEHQGLAP NeoR/KanR 150 155 160 165 MEAGLVDQDDLDEEHQGLAP NeoR/KanR MEAGLVDQDDLDEEHQGLAP HFGTKDILIIQVFLMLPERW t g g a a g c c g g t c t t g t c g a t c a g g a t g a t c t g g a c g a a g a g c a t c a g g g g c t c g c g c c a g a c c t t c g g c c a g a a c a g c t a g t c c t a c t a g a c c t g c t t c t c g t a g t c c c c g a g c g c g g t c

7140 170 175 180 185 AELFARLKASMPDGEDLVVT NeoR/KanR 170 175 180 185 AELFARLKASMPDGEDLVVT NeoR/KanR AELFARLKASMPDGEDLVVT GFQEGPELRAHGVALIEDHG c c g a a c t g t t c g c c a g g c t c a a g g c g a g c a t g c c c g a c g g c g a g g a t c t c g t c g t g a c c c g g c t t g a c a a g c g g t c c g a g t t c c g c t c g t a c g g g c t g c c g c t c c t a g a g c a g c a c t g g g

7200 190 195 200 205 HGDACLPNIMVENGRFSGFI NeoR/KanR 190 195 200 205 HGDACLPNIMVENGRFSGFI NeoR/KanR HGDACLPNIMVENGRFSGFI MAIGAQRIDHHFIAAKRSED a t g g c g a t g c c t g c t t g c c g a a t a t c a t g g t g g a a a a t g g c c g c t t t t c t g g a t t c a t c g t a c c g c t a c g g a c g a a c g g c t t a t a g t a c c a c c t t t t a c c g g c g a a a a g a c c t a a g t a g c

RsrII 7260 210 215 220 225 DCGRLGVADRYQDIALATRD NeoR/KanR 210 215 220 225 DCGRLGVADRYQDIALATRD NeoR/KanR C G T T G G C T A C C C G T G A T A Neo-F DCGRLGVADRYQDIALATRD VTAPQTHRVAILVYRQSGTI a c t g t g g c c g g c t g g g t g t g g c g g a c c g c t a t c a g g a c a t a g c g t t g g c t a c c c g t g a t a t g a c a c c g g c c g a c c c a c a c c g c c t g g c g a t a g t c c t g t a t c g c a a c c g a t g g g c a c t a t

7320 230 235 240 245 IAEELGGEWADRFLVLYGIA NeoR/KanR 230 235 240 245 IAEELGGEWADRFLVLYGIA NeoR/KanR T T Neo-F IAEELGGEWADRFLVLYGIA NSFLKAAFPSVAEEHKVTDG t t g c t g a a g a g c t t g g c g g c g a a t g g g c t g a c c g c t t c c t c g t g c t t t a c g g t a t c g c c g a a c g a c t t c t c g a a c c g c c g c t t a c c c g a c t g g c g a a g g a g c a c g a a a t g c c a t a g c g g c

7380 250 255 260 265 APDSQRIAFYRLLDEFF* NeoR/KanR 250 255 260 265 APDSQRIAFYRLLDEFF* NeoR/KanR APDSQRIAFYRLLDEFF* SGIRLADGEIAKKVLEESRS c t c c c g a t t c g c a g c g c a t c g c c t t c t a t c g c c t t c t t g a c g a g t t c t t c t g a g c g g g a c g a g g g c t a a g c g t c g c g t a g c g g a a g a t a g c g g a a g a a c t g c t c a a g a a g a c t c g c c c t g

BstBI 7440 MTDQATPNLPSRDFDS EPTRFSRGLSAWGAM t c t g g g g t t c g a a a t g a c c g a c c a a g c g a c g c c c a a c c t g c c a t c a c g a g a t t t c g a t t c a g a c c c c a a g c t t t a c t g g c t g g t t c g c t g c g g g t t g g a c g g t a g t g c t c t a a a g c t a a g

7500 TAAFYERLGFGIVFRDAGWM c a c c g c c g c c t t c t a t g a a a g g t t g g g c t t c g g a a t c g t t t t c c g g g a c g c c g g c t g g a t g t g g c g g c g g a a g a t a c t t t c c a a c c c g a a g c c t t a g c a a a a g g c c c t g c g g c c g a c c t a

7560 ILQRGDLMLEFFAHPRGRLT g a t c c t c c a g c g c g g g g a t c t c a t g c t g g a g t t c t t c g c c c a c c c t a g g g g g a g g c t a a c c t a g g a g g t c g c g c c c c t a g a g t a c g a c c t c a a g a a g c g g g t g g g a t c c c c c t c c g a t t g

7620 HSV TK poly(A) signal A C T T T G T G C C T T C C T C T G T T TK-pA-R ETRKETIPEGTRAMTAIKRQ t g a a a c a c g g a a g g a g a c a a t a c c g g a a g g a a c c c g c g c t a t g a c g g c a a t a a a a a g a c a a c t t t g t g c c t t c c t c t g t t a t g g c c t t c c t t g g g c g c g a t a c t g c c g t t a t t t t t c t g t

7680 HSV TK poly(A) signal NKTHGVGSFVHKRGVRSQGW g a a t a a a a c g c a c g g t g t t g g g t c g t t t g t t c a t a a a c g c g g g g t t c g g t c c c a g g g c t g c t t a t t t t g c g t g c c a c a a c c c a g c a a a c a a g t a t t t g c g c c c c a a g c c a g g g t c c c g a c

7740 HSVDTPPRPHWGQYARVSSF g c a c t c t g t c g a t a c c c c a c c g a g a c c c c a t t g g g g c c a a t a c g c c c g c g t t t c t t c c t t c g t g a g a c a g c t a t g g g g t g g c t c t g g g g t a a c c c c g g t t a t g c g g g c g c a a a g a a g g a a

7800 SPPHPPSSGEGPGLAANVGA t t c c c c a c c c c a c c c c c c a a g t t c g g g t g a a g g c c c a g g g c t c g c a g c c a a c g t c g g g g c a a g g g g t g g g g t g g g g g g t t c a a g c c c a c t t c c g g g t c c c g a g c g t c g g t t g c a g c c c c g

7860 AGPAIASGYSYIL* g g c a g g c c c t g c c a t a g c c t c a g g t t a c t c a t a t a t a c t t t a g a t t g a t t t a a a a c t t c a c c g t c c g g g a c g g t a t c g g a g t c c a a t g a g t a t a t a t g a a a t c t a a c t a a a t t t t g a a g t

7920 t t t t t a a t t t a a a a g g a t c t a g g t g a a g a t c c t t t t t g a t a a t c t c a t g a c c a a a a t c c c a a a a a t t a a a t t t t c c t a g a t c c a c t t c t a g g a a a a a c t a t t a g a g t a c t g g t t t t a g g g

7980 t t a a c g t g a g t t t t c g t t c c a c t g a g c g t c a g a c c c c g t a g a a a a g a t c a a a g g a t c t t c a a t t g c a c t c a a a a g c a a g g t g a c t c g c a g t c t g g g g c a t c t t t t c t a g t t t c c t a g a a g

8040 ori t t g a g a t c c t t t t t t t c t g c g c g t a a t c t g c t g c t t g c a a a c a a a a a a a c c a c c g c t a c c a a c t c t a g g a a a a a a a g a c g c g c a t t a g a c g a c g a a c g t t t g t t t t t t t g g t g g c g a t g g

8100 ori a g c g g t g g t t t g t t t g c c g g a t c a a g a g c t a c c a a c t c t t t t t c c g a a g g t a a c t g g c t t t c g c c a c c a a a c a a a c g g c c t a g t t c t c g a t g g t t g a g a a a a a g g c t t c c a t t g a c c g a a

8160 ori c a g c a g a g c g c a g a t a c c a a a t a c t g t t c t t c t a g t g t a g c c g t a g t t a g g c c a c c a c t t g t c g t c t c g c g t c t a t g g t t t a t g a c a a g a a g a t c a c a t c g g c a t c a a t c c g g t g g t g a a

8220 ori c a a g a a c t c t g t a g c a c c g c c t a c a t a c c t c g c t c t g c t a a t c c t g t t a c c a g t g g c t g c g t t c t t g a g a c a t c g t g g c g g a t g t a t g g a g c g a g a c g a t t a g g a c a a t g g t c a c c g a c g

8280 ori t g c c a g t g g c g a t a a g t c g t g t c t t a c c g g g t t g g a c t c a a g a c g a t a g t t a c c g g a t a a a c g g t c a c c g c t a t t c a g c a c a g a a t g g c c c a a c c t g a g t t c t g c t a t c a a t g g c c t a t t

ApaLI 8340 ori g g c g c a g c g g t c g g g c t g a a c g g g g g g t t c g t g c a c a c a g c c c a g c t t g g a g c g a a c g a c c c g c g t c g c c a g c c c g a c t t g c c c c c c a a g c a c g t g t g t c g g g t c g a a c c t c g c t t g c t g

8400 ori c t a c a c c g a a c t g a g a t a c c t a c a g c g t g a g c t a t g a g a a a g c g c c a c g c t t c c c g a a g g g a t g t g g c t t g a c t c t a t g g a t g t c g c a c t c g a t a c t c t t t c g c g g t g c g a a g g g c t t c c

8460 ori g a g a a a g g c g g a c a g g t a t c c g g t a a g c g g c a g g g t c g g a a c a g g a g a g c g c a c g a g g g a c t c t t t c c g c c t g t c c a t a g g c c a t t c g c c g t c c c a g c c t t g t c c t c t c g c g t g c t c c c t

8520 ori G G G A A A C G C C T G G T A T C T T T pBR322ori-F g c t t c c a g g g g g a a a c g c c t g g t a t c t t t a t a g t c c t g t c g g g t t t c g c c a c c t c t g a c t c g a a g g t c c c c c t t t g c g g a c c a t a g a a a t a t c a g g a c a g c c c a a a g c g g t g g a g a c t g a

8580 ori t g a g c g t c g a t t t t t g t g a t g c t c g t c a g g g g g g c g g a g c c t a t g g a a a a a c g c c a g c a a a c t c g c a g c t a a a a a c a c t a c g a g c a g t c c c c c c g c c t c g g a t a c c t t t t t g c g g t c g t t

PciI 8640 c g c g g c c t t t t t a c g g t t c c t g g c c t t t t g c t g g c c t t t t g c t c a c a t g t t c t t t c c t g c g c g c c g g a a a a a t g c c a a g g a c c g g a a a a c g a c c g g a a a a c g a g t g t a c a a g a a a g g a c g

3ʹ 5ʹ 8690 g t t a t c c c c t g a t t c t g t g g a t a a c c g t a t t a c c g c c a t g c a t t a g t t a t c a a t a g g g g a c t a a g a c a c c t a t t g g c a t a a t g g c g g t a c g t a a t c a a t a

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.