Addgene pPHR-iRFP713-NLS-VP16 - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pPHR-iRFP713-NLS-VP16

Map View

pBR322ori-F (6514 .. 6533) ApaLI (6355) TK-pA-R (5605 .. 5624) BstBI (5433) Neo-F (5287 .. 5306) RsrII (5267) PflFI - Tth111I (4869) Neo-R (4677 .. 4696) BspDI * - ClaI * (4591) SfiI (4526) CsiI - SexAI * (4340) pBABE 3' (4254 .. 4274) F1ori-R (3992 .. 4011) DraIII (3867) F1ori-F (3780 .. 3801) AflII (3633) EBV-rev (3606 .. 3625) BtsI - BtsαI (3590) SV40pA-R (3552 .. 3571) MfeI (3501) NotI (3395) AseI (7) NdeI (234) SnaBI (340) CMV-F (519 .. 539) NheI (591) BmtI (595) AfeI (596) XcmI (627) BclI * (2457) SbfI (2555) SacII (2591) EcoRV (2790) PflMI * (3045) BsrGI (3073) SalI (3086) Acc65I (3094) KpnI (3098) PshAI (3166) BmgBI (3207) pPHR-iRFP713-NLS-VP16 6727 bp

Sequence View

AseI 5ʹ 3ʹ 60 t a g t t a t t a a t a g t a a t c a a t t a c g g g g t c a t t a g t t c a t a g c c c a t a t a t g g a g t t c c g a t c a a t a a t t a t c a t t a g t t a a t g c c c c a g t a a t c a a g t a t c g g g t a t a t a c c t c a a g g c

120 CMV enhancer c g t t a c a t a a c t t a c g g t a a a t g g c c c g c c t g g c t g a c c g c c c a a c g a c c c c c g c c c a t t g c a a t g t a t t g a a t g c c a t t t a c c g g g c g g a c c g a c t g g c g g g t t g c t g g g g g c g g g t a a

180 CMV enhancer g a c g t c a a t a a t g a c g t a t g t t c c c a t a g t a a c g c c a a t a g g g a c t t t c c a t t g a c g t c a c t g c a g t t a t t a c t g c a t a c a a g g g t a t c a t t g c g g t t a t c c c t g a a a g g t a a c t g c a g t

NdeI 240 CMV enhancer a t g g g t g g a g t a t t t a c g g t a a a c t g c c c a c t t g g c a g t a c a t c a a g t g t a t c a t a t g c c t a c c c a c c t c a t a a a t g c c a t t t g a c g g g t g a a c c g t c a t g t a g t t c a c a t a g t a t a c g g

300 CMV enhancer a a g t a c g c c c c c t a t t g a c g t c a a t g a c g g t a a a t g g c c c g c c t g g c a t t a t g c c c a g t a t t c a t g c g g g g g a t a a c t g c a g t t a c t g c c a t t t a c c g g g c g g a c c g t a a t a c g g g t c a t

SnaBI 360 CMV enhancer c a t g a c c t t a t g g g a c t t t c c t a c t t g g c a g t a c a t c t a c g t a t t a g t c a t c g c t a t t a c g t a c t g g a a t a c c c t g a a a g g a t g a a c c g t c a t g t a g a t g c a t a a t c a g t a g c g a t a a t g

420 CMV enhancer CMV promoter c a t g g t g a t g c g g t t t t g g c a g t a c a t c a a t g g g c g t g g a t a g c g g t t t g a c t c a c g g g g g t a c c a c t a c g c c a a a a c c g t c a t g t a g t t a c c c g c a c c t a t c g c c a a a c t g a g t g c c c c

480 CMV promoter a t t t c c a a g t c t c c a c c c c a t t g a c g t c a a t g g g a g t t t g t t t t g g c a c c a a a a t c a a c g t a a a g g t t c a g a g g t g g g g t a a c t g c a g t t a c c c t c a a a c a a a a c c g t g g t t t t a g t t g c

540 CMV promoter C G C A A A T G G G C G G T A G G C G T G CMV-F g g a c t t t c c a a a a t g t c g t a a c a a c t c c g c c c c a t t g a c g c a a a t g g g c g g t a g g c g t g t c c t g a a a g g t t t t a c a g c a t t g t t g a g g c g g g g t a a c t g c g t t t a c c c g c c a t c c g c a c a

NheIBmtIAfeI 600 CMV promoter a c g g t g g g a g g t c t a t a t a a g c a g a g c t g g t t t a g t g a a c c g t c a g a t c c g c t a g c g c t a t g c c a c c c t c c a g a t a t a t t c g t c t c g a c c a a a t c a c t t g g c a g t c t a g g c g a t c g c g a t

XcmI 660 1 5 10 MKMDKKTIVWFR CRY2PHR MKMDKKTIVWFR c c g g a c t c a g a t c t c g a g g c c a c c a t g a a g a t g g a c a a a a a g a c t a t a g t t t g g t t t a g a g g c c t g a g t c t a g a g c t c c g g t g g t a c t t c t a c c t g t t t t t c t g a t a t c a a a c c a a a t c t

720 15 20 25 30 RDLRIEDNPALAAAAHEGSV CRY2PHR RDLRIEDNPALAAAAHEGSV a g a g a c c t a a g g a t t g a g g a t a a t c c t g c a t t a g c a g c a g c t g c t c a c g a a g g a t c t g t t t c t c t g g a t t c c t a a c t c c t a t t a g g a c g t a a t c g t c g t c g a c g a g t g c t t c c t a g a c a a

780 35 40 45 50 FPVFIWCPEEEGQFYPGRAS CRY2PHR FPVFIWCPEEEGQFYPGRAS t t t c c t g t c t t c a t t t g g t g t c c t g a a g a a g a a g g a c a g t t t t a t c c t g g a a g a g c t t c a a a a g g a c a g a a g t a a a c c a c a g g a c t t c t t c t t c c t g t c a a a a t a g g a c c t t c t c g a a g t

840 55 60 65 70 RWWMKQSLAHLSQSLKALGS CRY2PHR RWWMKQSLAHLSQSLKALGS a g a t g g t g g a t g a a a c a a t c a c t t g c t c a c t t a t c t c a a t c c t t g a a g g c t c t t g g a t c t t c t a c c a c c t a c t t t g t t a g t g a a c g a g t g a a t a g a g t t a g g a a c t t c c g a g a a c c t a g a

900 75 80 85 90 DLTLIKTHNTISAILDCIRV CRY2PHR DLTLIKTHNTISAILDCIRV g a c c t c a c t t t a a t c a a a a c c c a c a a c a c g a t t t c a g c g a t c t t g g a t t g t a t c c g c g t t c t g g a g t g a a a t t a g t t t t g g g t g t t g t g c t a a a g t c g c t a g a a c c t a a c a t a g g c g c a a

960 95 100 105 110 TGATKVVFNHLYDPVSLVRD CRY2PHR TGATKVVFNHLYDPVSLVRD a c c g g t g c t a c a a a a g t c g t c t t t a a c c a c c t c t a t g a t c c t g t t t c g t t a g t t c g g g a c t g g c c a c g a t g t t t t c a g c a g a a a t t g g t g g a g a t a c t a g g a c a a a g c a a t c a a g c c c t g

1020 115 120 125 130 HTVKEKLVERGISVQSYNGD CRY2PHR HTVKEKLVERGISVQSYNGD c a t a c c g t a a a g g a g a a g c t g g t g g a a c g t g g g a t c t c t g t g c a a a g c t a c a a t g g a g a t g t a t g g c a t t t c c t c t t c g a c c a c c t t g c a c c c t a g a g a c a c g t t t c g a t g t t a c c t c t a

1080 135 140 145 150 LLYEPWEIYCEKGKPFTSFN CRY2PHR LLYEPWEIYCEKGKPFTSFN c t a t t g t a t g a a c c g t g g g a g a t a t a c t g c g a a a a g g g c a a a c c t t t t a c g a g t t t c a a t g a t a a c a t a c t t g g c a c c c t c t a t a t g a c g c t t t t c c c g t t t g g a a a a t g c t c a a a g t t a

1140 155 160 165 170 SYWKKCLDMSIESVMLPPPW CRY2PHR SYWKKCLDMSIESVMLPPPW t c t t a c t g g a a g a a a t g c t t a g a t a t g t c g a t t g a a t c c g t t a t g c t t c c t c c t c c t t g g a g a a t g a c c t t c t t t a c g a a t c t a t a c a g c t a a c t t a g g c a a t a c g a a g g a g g a g g a a c c

1200 175 180 185 190 RLMPITAAAEAIWACSIEEL CRY2PHR RLMPITAAAEAIWACSIEEL c g g t t g a t g c c a a t a a c t g c a g c g g c t g a a g c g a t t t g g g c g t g t t c g a t t g a a g a a c t a g c c a a c t a c g g t t a t t g a c g t c g c c g a c t t c g c t a a a c c c g c a c a a g c t a a c t t c t t g a t

1260 195 200 205 210 GLENEAEKPSNALLTRAWSP CRY2PHR GLENEAEKPSNALLTRAWSP g g g c t g g a g a a t g a g g c c g a g a a a c c g a g c a a t g c g t t g t t a a c t a g a g c t t g g t c t c c a c c c g a c c t c t t a c t c c g g c t c t t t g g c t c g t t a c g c a a c a a t t g a t c t c g a a c c a g a g g t

1320 215 220 225 230 GWSNADKLLNEFIEKQLIDY CRY2PHR GWSNADKLLNEFIEKQLIDY g g a t g g a g c a a t g c t g a t a a g t t a c t a a a t g a g t t c a t c g a g a a g c a g t t g a t a g a t t a t c c t a c c t c g t t a c g a c t a t t c a a t g a t t t a c t c a a g t a g c t c t t c g t c a a c t a t c t a a t a

1380 235 240 245 250 AKNSKKVVGNSTSLLSPYLH CRY2PHR AKNSKKVVGNSTSLLSPYLH g c a a a g a a c a g c a a g a a a g t t g t t g g g a a t t c t a c t t c a c t a c t t t c t c c g t a t c t c c a t c g t t t c t t g t c g t t c t t t c a a c a a c c c t t a a g a t g a a g t g a t g a a a g a g g c a t a g a g g t a

1440 255 260 265 270 FGEISVRHVFQCARMKQIIW CRY2PHR FGEISVRHVFQCARMKQIIW t t c g g g g a a a t a a g c g t c a g a c a c g t t t t c c a g t g t g c c c g g a t g a a a c a a a t t a t a t g g a a g c c c c t t t a t t c g c a g t c t g t g c a a a a g g t c a c a c g g g c c t a c t t t g t t t a a t a t a c c

1500 275 280 285 290 ARDKNSEGEESADLFLRGIG CRY2PHR ARDKNSEGEESADLFLRGIG g c a a g a g a t a a g a a c a g t g a a g g a g a a g a a a g t g c a g a t c t t t t t c t t a g g g g a a t c g g t c g t t c t c t a t t c t t g t c a c t t c c t c t t c t t t c a c g t c t a g a a a a a g a a t c c c c t t a g c c a

1560 295 300 305 310 LREYSRYICFNFPFTHEQSL CRY2PHR LREYSRYICFNFPFTHEQSL t t a a g a g a g t a t t c t c g g t a t a t a t g t t t c a a c t t c c c g t t t a c t c a c g a g c a a t c g t t g a a t t c t c t c a t a a g a g c c a t a t a t a c a a a g t t g a a g g g c a a a t g a g t g c t c g t t a g c a a c

1620 315 320 325 330 LSHLRFFPWDADVDKFKAWR CRY2PHR LSHLRFFPWDADVDKFKAWR t t g a g t c a t c t t c g g t t t t t c c c t t g g g a t g c t g a t g t t g a t a a g t t c a a g g c c t g g a g a a a c t c a g t a g a a g c c a a a a a g g g a a c c c t a c g a c t a c a a c t a t t c a a g t t c c g g a c c t c t

1680 335 340 345 350 QGRTGYPLVDAGMRELWATG CRY2PHR QGRTGYPLVDAGMRELWATG c a a g g c a g g a c c g g t t a t c c g t t g g t g g a t g c c g g a a t g a g a g a g c t t t g g g c t a c c g g a g t t c c g t c c t g g c c a a t a g g c a a c c a c c t a c g g c c t t a c t c t c t c g a a a c c c g a t g g c c t

1740 355 360 365 370 WMHNRIRVIVSSFAVKFLLL CRY2PHR WMHNRIRVIVSSFAVKFLLL t g g a t g c a t a a c a g a a t a a g a g t g a t t g t t t c a a g c t t t g c t g t g a a g t t t c t t c t c c t t a c c t a c g t a t t g t c t t a t t c t c a c t a a c a a a g t t c g a a a c g a c a c t t c a a a g a a g a g g a a

1800 375 380 385 390 PWKWGMKYFWDTLLDADLEC CRY2PHR PWKWGMKYFWDTLLDADLEC c c a t g g a a a t g g g g a a t g a a g t a t t t c t g g g a t a c a c t t t t g g a t g c t g a t t t g g a a t g t g g t a c c t t t a c c c c t t a c t t c a t a a a g a c c c t a t g t g a a a a c c t a c g a c t a a a c c t t a c a

1860 395 400 405 410 DILGWQYISGSIPDGHELDR CRY2PHR DILGWQYISGSIPDGHELDR g a c a t c c t t g g c t g g c a g t a t a t c t c t g g g a g t a t c c c c g a t g g c c a c g a g c t t g a t c g c c t g t a g g a a c c g a c c g t c a t a t a g a g a c c c t c a t a g g g g c t a c c g g t g c t c g a a c t a g c g

1920 415 420 425 430 LDNPALQGAKYDPEGEYIRQ CRY2PHR LDNPALQGAKYDPEGEYIRQ t t g g a c a a t c c c g c g t t a c a a g g c g c c a a a t a t g a c c c a g a a g g t g a g t a c a t a a g g c a a a a c c t g t t a g g g c g c a a t g t t c c g c g g t t t a t a c t g g g t c t t c c a c t c a t g t a t t c c g t t

1980 435 440 445 450 WLPELARLPTEWIHHPWDAP CRY2PHR WLPELARLPTEWIHHPWDAP t g g c t t c c c g a g c t t g c g a g a t t g c c a a c t g a a t g g a t c c a t c a t c c a t g g g a c g c t c c t a c c g a a g g g c t c g a a c g c t c t a a c g g t t g a c t t a c c t a g g t a g t a g g t a c c c t g c g a g g a

2040 455 460 465 470 LTVLKASGVELGTNYAKPIV CRY2PHR LTVLKASGVELGTNYAKPIV t t a a c c g t a c t c a a a g c t t c t g g t g t g g a a c t c g g a a c a a a c t a t g c g a a a c c c a t t g t a a a t t g g c a t g a g t t t c g a a g a c c a c a c c t t g a g c c t t g t t t g a t a c g c t t t g g g t a a c a t

2100 475 480 485 490 DIDTARELLAKAISRTREAQ CRY2PHR DIDTARELLAKAISRTREAQ g a c a t c g a c a c a g c t c g t g a g c t a c t a g c t a a a g c t a t t t c a a g a a c c c g t g a a g c a c a g c t g t a g c t g t g t c g a g c a c t c g a t g a t c g a t t t c g a t a a a g t t c t t g g g c a c t t c g t g t c

2160 495 IMIGAA ARL 1 5 10 MAEGSVARQPD CRY2PHR iRFP713 IMIGAAARLMAEGSVARQPD a t c a t g a t c g g a g c a g c a g c c c g g t t a a t g g c t g a a g g a t c c g t c g c c a g g c a g c c t g a c t a g t a c t a g c c t c g t c g t c g g g c c a a t t a c c g a c t t c c t a g g c a g c g g t c c g t c g g a c t g

2220 15 20 25 30 LLTCDDEPIHIPGAIQPHGL iRFP713 LLTCDDEPIHIPGAIQPHGL c t c t t g a c c t g c g a c g a t g a g c c g a t c c a t a t c c c c g g t g c c a t c c a a c c g c a t g g a c t g g a g a a c t g g a c g c t g c t a c t c g g c t a g g t a t a g g g g c c a c g g t a g g t t g g c g t a c c t g a c

2280 35 40 45 50 LLALAADMTIVAGSDNLPEL iRFP713 LLALAADMTIVAGSDNLPEL c t g c t c g c c c t c g c c g c c g a c a t g a c g a t c g t t g c c g g c a g c g a c a a c c t t c c c g a a c t c g a c g a g c g g g a g c g g c g g c t g t a c t g c t a g c a a c g g c c g t c g c t g t t g g a a g g g c t t g a g

2340 55 60 65 70 TGLAIGALIGRSAADVFDSE iRFP713 TGLAIGALIGRSAADVFDSE a c c g g a c t g g c g a t c g g c g c c c t g a t c g g c c g c t c t g c g g c c g a t g t c t t c g a c t c g g a g t g g c c t g a c c g c t a g c c g c g g g a c t a g c c g g c g a g a c g c c g g c t a c a g a a g c t g a g c c t c

2400 75 80 85 90 THNRLTIALAEPGAAVGAPI iRFP713 THNRLTIALAEPGAAVGAPI a c g c a c a a c c g t c t g a c g a t c g c c t t g g c c g a g c c c g g g g c g g c c g t c g g a g c a c c g a t c t g c g t g t t g g c a g a c t g c t a g c g g a a c c g g c t c g g g c c c c g c c g g c a g c c t c g t g g c t a g

BclI* 2460 95 100 105 110 TVGFTMRKDAGFIGSWHRHD iRFP713 TVGFTMRKDAGFIGSWHRHD a c t g t c g g c t t c a c g a t g c g a a a g g a c g c a g g c t t c a t c g g c t c c t g g c a t c g c c a t g a t t g a c a g c c g a a g t g c t a c g c t t t c c t g c g t c c g a a g t a g c c g a g g a c c g t a g c g g t a c t a

2520 115 120 125 130 QLIFLELEPPQRDVAEPQAF iRFP713 QLIFLELEPPQRDVAEPQAF c a g c t c a t c t t c c t c g a g c t c g a g c c t c c c c a g c g g g a c g t c g c c g a g c c g c a g g c g t t c g t c g a g t a g a a g g a g c t c g a g c t c g g a g g g g t c g c c c t g c a g c g g c t c g g c g t c c g c a a g

SbfI 2580 135 140 145 150 FRRTNSAIRRLQAAETLESA iRFP713 FRRTNSAIRRLQAAETLESA t t c c g c c g c a c c a a c a g c g c c a t c c g c c g c c t g c a g g c c g c c g a a a c c t t g g a a a g c g c c a a g g c g g c g t g g t t g t c g c g g t a g g c g g c g g a c g t c c g g c g g c t t t g g a a c c t t t c g c g g

SacII 2640 155 160 165 170 CAAAAQEVRKITGFDRVMIY iRFP713 CAAAAQEVRKITGFDRVMIY t g c g c c g c c g c g g c g c a a g a g g t g c g g a a g a t t a c c g g c t t c g a t c g g g t g a t g a t c t a t a c g c g g c g g c g c c g c g t t c t c c a c g c c t t c t a a t g g c c g a a g c t a g c c c a c t a c t a g a t a

2700 175 180 185 190 RFASDFSGEVIAEDRCAEVE iRFP713 RFASDFSGEVIAEDRCAEVE c g c t t c g c c t c c g a c t t c a g c g g c g a a g t g a t c g c a g a g g a t c g g t g c g c c g a g g t c g a g g c g a a g c g g a g g c t g a a g t c g c c g c t t c a c t a g c g t c t c c t a g c c a c g c g g c t c c a g c t c

2760 195 200 205 210 SKLGLHYPASTVPAQARRLY iRFP713 SKLGLHYPASTVPAQARRLY t c a a a a c t a g g c c t g c a c t a t c c t g c c t c a a c c g t g c c g g c g c a g g c c c g t c g g c t c t a t a g t t t t g a t c c g g a c g t g a t a g g a c g g a g t t g g c a c g g c c g c g t c c g g g c a g c c g a g a t a

EcoRV 2820 215 220 225 230 TINPVRIIPDINYRPVPVTP iRFP713 TINPVRIIPDINYRPVPVTP a c c a t c a a c c c g g t a c g g a t c a t t c c c g a t a t c a a t t a t c g g c c g g t g c c g g t c a c c c c a t g g t a g t t g g g c c a t g c c t a g t a a g g g c t a t a g t t a a t a g c c g g c c a c g g c c a g t g g g g t

2880 235 240 245 250 DLNPVTGRPIDLSFAILRSV iRFP713 DLNPVTGRPIDLSFAILRSV g a c c t c a a t c c g g t c a c c g g g c g g c c g a t t g a t c t t a g c t t c g c c a t c c t g c g c a g c g t c c t g g a g t t a g g c c a g t g g c c c g c c g g c t a a c t a g a a t c g a a g c g g t a g g a c g c g t c g c a g

2940 255 260 265 270 SPVHLEFMRNIGMHGTMSIS iRFP713 SPVHLEFMRNIGMHGTMSIS t c g c c c g t c c a t c t g g a a t t c a t g c g c a a c a t a g g c a t g c a c g g c a c g a t g t c g a t c t c g a g c g g g c a g g t a g a c c t t a a g t a c g c g t t g t a t c c g t a c g t g c c g t g c t a c a g c t a g a g c

3000 275 280 285 290 ILRGERLWGLIVCHHRTPYY iRFP713 ILRGERLWGLIVCHHRTPYY a t t t t g c g c g g c g a g c g a c t g t g g g g a t t g a t c g t t t g c c a t c a c c g a a c g c c g t a c t a c t a a a a c g c g c c g c t c g c t g a c a c c c c t a a c t a g c a a a c g g t a g t g g c t t g c g g c a t g a t g

PflMI* 3060 295 300 305 310 VDLDGRQACELVAQVLAWQI iRFP713 VDLDGRQACELVAQVLAWQI g t c g a t c t c g a t g g c c g c c a a g c c t g c g a g c t a g t c g c c c a g g t t c t g g c c t g g c a g a t c c a g c t a g a g c t a c c g g c g g t t c g g a c g c t c g a t c a g c g g g t c c a a g a c c g g a c c g t c t a g

Acc65IKpnISalIBsrGI 3120 315 GVME (in frame with iRFP713) CTRGRRLGTP 130 PKKKRK iRFP713 SV40 NLS GVMECTRGRRLGTPPKKKRK g g c g t g a t g g a a t g t a c a a g g g g g c g t c g a c t g g g t a c c c c t c c t a a g a a g a a g a g g a a g c c g c a c t a c c t t a c a t g t t c c c c c g c a g c t g a c c c a t g g g g a g g a t t c t t c t t c t c c t t c

PshAI 3180 V (in frame with SV40 NLS) APTMDKKKRKAPPTDVSLG SV40 NLS VAPTMDKKKRKAPPTDVSLG g t t g c c c c c a c c a t g g a t a a a a a g a a g a g a a a g g c c c c c c c g a c c g a t g t c a g c c t g g g g c a a c g g g g g t g g t a c c t a t t t t t c t t c t c t t t c c g g g g g g g c t g g c t a c a g t c g g a c c c c

BmgBI 3240 (in frame with SV40 NLS) DELHLDGEDVAMAHADALDD DELHLDGEDVAMAHADALDD g a c g a g c t c c a c t t a g a c g g c g a g g a c g t g g c g a t g g c g c a t g c c g a c g c g c t a g a c g a t c t g c t c g a g g t g a a t c t g c c g c t c c t g c a c c g c t a c c g c g t a c g g c t g c g c g a t c t g c t a

3300 (in frame with SV40 NLS) FDLDMLGDGDSPGPGFTPHD FDLDMLGDGDSPGPGFTPHD t t c g a t c t g g a c a t g t t g g g g g a c g g g g a t t c c c c g g g t c c g g g a t t t a c c c c c c a c g a c a a g c t a g a c c t g t a c a a c c c c c t g c c c c t a a g g g g c c c a g g c c c t a a a t g g g g g g t g c t g

3360 (in frame with SV40 NLS) SAPYGALDMADFEFEQMFTD SAPYGALDMADFEFEQMFTD t c c g c c c c c t a c g g c g c t c t g g a t a t g g c c g a c t t c g a g t t t g a g c a g a t g t t t a c c g a t a g g c g g g g g a t g c c g c g a g a c c t a t a c c g g c t g a a g c t c a a a c t c g t c t a c a a a t g g c t a

NotI 3420 (in frame with SV40 NLS) APLDGGIHRI* APLDGGIHRI* g c c c c t c t a g a c g g g g g g a t c c a c c g g a t c t a g g c g g c c g c g a c t c t a g a t c a t a a t c a g c g g g g a g a t c t g c c c c c c t a g g t g g c c t a g a t c c g c c g g c g c t g a g a t c t a g t a t t a g t c

3480 c c a t a c c a c a t t t g t a g a g g t t t t a c t t g c t t t a a a a a a c c t c c c a c a c c t c c c c c t g a a g g t a t g g t g t a a a c a t c t c c a a a a t g a a c g a a a t t t t t t g g a g g g t g t g g a g g g g g a c t t

MfeI 3540 SV40 poly(A) signal c c t g a a a c a t a a a a t g a a t g c a a t t g t t g t t g t t a a c t t g t t t a t t g c a g c t t a t a a t g g g g a c t t t g t a t t t t a c t t a c g t t a a c a a c a a c a a t t g a a c a a a t a a c g t c g a a t a t t a c c

BtsαIBtsI 3600 SV40 poly(A) signal C G T T A T C G T A G T G T T T A A A G SV40pA-R t t a c a a a t a a a g c a a t a g c a t c a c a a a t t t c a c a a a t a a a g c a t t t t t t t c a c t g c a t t c a a t g t t t a t t t c g t t a t c g t a g t g t t t a a a g t g t t t a t t t c g t a a a a a a a g t g a c g t a a g

AflII 3660 SV40 poly(A) signal f1 ori G T G G T T T G T C C A A A C T C A T C EBV-rev t a g t t g t g g t t t g t c c a a a c t c a t c a a t g t a t c t t a a g g c g t a a a t t g t a a g c g t t a a t a a t c a a c a c c a a a c a g g t t t g a g t a g t t a c a t a g a a t t c c g c a t t t a a c a t t c g c a a t t a t

3720 f1 ori t t t t g t t a a a a t t c g c g t t a a a t t t t t g t t a a a t c a g c t c a t t t t t t a a c c a a t a g g c c g a a a a c a a t t t t a a g c g c a a t t t a a a a a c a a t t t a g t c g a g t a a a a a a t t g g t t a t c c g g c

3780 f1 ori G F1ori-F a a a t c g g c a a a a t c c c t t a t a a a t c a a a a g a a t a g a c c g a g a t a g g g t t g a g t g t t g t t c t t t a g c c g t t t t a g g g a a t a t t t a g t t t t c t t a t c t g g c t c t a t c c c a a c t c a c a a c a a g

3840 f1 ori G T C A A A C C T T G T T C T C A G G T G F1ori-F c a g t t t g g a a c a a g a g t c c a c t a t t a a a g a a c g t g g a c t c c a a c g t c a a a g g g c g a a a a a g t c a a a c c t t g t t c t c a g g t g a t a a t t t c t t g c a c c t g a g g t t g c a g t t t c c c g c t t t t t

DraIII 3900 f1 ori c c g t c t a t c a g g g c g a t g g c c c a c t a c g t g a a c c a t c a c c c t a a t c a a g t t t t t t g g g g t g g c a g a t a g t c c c g c t a c c g g g t g a t g c a c t t g g t a g t g g g a t t a g t t c a a a a a a c c c c a

3960 f1 ori c g a g g t g c c g t a a a g c a c t a a a t c g g a a c c c t a a a g g g a g c c c c c g a t t t a g a g c t t g a c g c t c c a c g g c a t t t c g t g a t t t a g c c t t g g g a t t t c c c t c g g g g g c t a a a t c t c g a a c t g

4020 f1 ori A G G G A A G A A A G C G A A A G G A G F1ori-R g g g g a a a g c c g g c g a a c g t g g c g a g a a a g g a a g g g a a g a a a g c g a a a g g a g c g g g c g c t a c c c c t t t c g g c c g c t t g c a c c g c t c t t t c c t t c c c t t c t t t c g c t t t c c t c g c c c g c g a t

4080 f1 ori g g g c g c t g g c a a g t g t a g c g g t c a c g c t g c g c g t a a c c a c c a c a c c c g c c g c g c t t a a t g c c c g c g a c c g t t c a c a t c g c c a g t g c g a c g c g c a t t g g t g g t g t g g g c g g c g c g a a t t a c

4140 f1 ori AmpR promoter c g c c g c t a c a g g g c g c g t c a g g t g g c a c t t t t c g g g g a a a t g t g c g c g g a a c c c c t a t t t g c g g c g a t g t c c c g c g c a g t c c a c c g t g a a a a g c c c c t t t a c a c g c g c c t t g g g g a t a a a

4200 AmpR promoter g t t t a t t t t t c t a a a t a c a t t c a a a t a t g t a t c c g c t c a t g a g a c a a t a a c c c t g a t a a a c a a a t a a a a a g a t t t a t g t a a g t t t a t a c a t a g g c g a g t a c t c t g t t a t t g g g a c t a t t t

4260 AmpR promoter SV40 promoter SV40 enhancer C C T T A C A pBABE 3' t g c t t c a a t a a t a t t g a a a a a g g a a g a g t c c t g a g g c g g a a a g a a c c a g c t g t g g a a t g t a c g a a g t t a t t a t a a c t t t t t c c t t c t c a g g a c t c c g c c t t t c t t g g t c g a c a c c t t a c a

4320 SV40 promoter SV40 enhancer C A C A G T C A A T C C C A pBABE 3' g t g t c a g t t a g g g t g t g g a a a g t c c c c a g g c t c c c c a g c a g g c a g a a g t a t g c a a a g c a t c a c a g t c a a t c c c a c a c c t t t c a g g g g t c c g a g g g g t c g t c c g t c t t c a t a c g t t t c g t a

SexAI*CsiI 4380 SV40 promoter SV40 enhancer g c a t c t c a a t t a g t c a g c a a c c a g g t g t g g a a a g t c c c c a g g c t c c c c a g c a g g c a g a a g c g t a g a g t t a a t c a g t c g t t g g t c c a c a c c t t t c a g g g g t c c g a g g g g t c g t c c g t c t t c

4440 SV40 promoter SV40 enhancer SV40 ori t a t g c a a a g c a t g c a t c t c a a t t a g t c a g c a a c c a t a g t c c c g c c c c t a a c t c c g c c c a t a t a c g t t t c g t a c g t a g a g t t a a t c a g t c g t t g g t a t c a g g g c g g g g a t t g a g g c g g g t a

4500 SV40 promoter SV40 ori c c c g c c c c t a a c t c c g c c c a g t t c c g c c c a t t c t c c g c c c c a t g g c t g a c t a a t t t t t t t g g g c g g g g a t t g a g g c g g g t c a a g g c g g g t a a g a g g c g g g g t a c c g a c t g a t t a a a a a a a

SfiI 4560 SV40 promoter SV40 ori t a t t t a t g c a g a g g c c g a g g c c g c c t c g g c c t c t g a g c t a t t c c a g a a g t a g t g a g g a g g a t a a a t a c g t c t c c g g c t c c g g c g g a g c c g g a g a c t c g a t a a g g t c t t c a t c a c t c c t c c

BspDI*ClaI* 4620 SV40 promoter SV40 ori c t t t t t t g g a g g c c t a g g c t t t t g c a a a g a t c g a t c a a g a g a c a g g a t g a g g a t c g t t t c g a a a a a a c c t c c g g a t c c g a a a a c g t t t c t a g c t a g t t c t c t g t c c t a c t c c t a g c a a a g

4680 1 5 10 15 MIEQDGLHAGSPAAWVERL NeoR/KanR 1 5 10 15 MIEQDGLHAGSPAAWVERL NeoR/KanR G A T A Neo-R MIEQDGLHAGSPAAWVERL g c a t g a t t g a a c a a g a t g g a t t g c a c g c a g g t t c t c c g g c c g c t t g g g t g g a g a g g c t a t c g t a c t a a c t t g t t c t a c c t a a c g t g c g t c c a a g a g g c c g g c g a a c c c a c c t c t c c g a t a

4740 20 25 30 35 FGYDWAQQTIGCSDAAVFRL NeoR/KanR 20 25 30 35 FGYDWAQQTIGCSDAAVFRL NeoR/KanR A G C C G A T A C T G A C C C G Neo-R FGYDWAQQTIGCSDAAVFRL t c g g c t a t g a c t g g g c a c a a c a g a c a a t c g g c t g c t c t g a t g c c g c c g t g t t c c g g c t g t a g c c g a t a c t g a c c c g t g t t g t c t g t t a g c c g a c g a g a c t a c g g c g g c a c a a g g c c g a c a

4800 40 45 50 55 SAQGRPVLFVKTDLSGALNE NeoR/KanR 40 45 50 55 SAQGRPVLFVKTDLSGALNE NeoR/KanR SAQGRPVLFVKTDLSGALNE c a g c g c a g g g g c g c c c g g t t c t t t t t g t c a a g a c c g a c c t g t c c g g t g c c c t g a a t g a a c g t c g c g t c c c c g c g g g c c a a g a a a a a c a g t t c t g g c t g g a c a g g c c a c g g g a c t t a c t t g

4860 60 65 70 75 LQDEAARLSWLATTGVPCAA NeoR/KanR 60 65 70 75 LQDEAARLSWLATTGVPCAA NeoR/KanR LQDEAARLSWLATTGVPCAA t g c a a g a c g a g g c a g c g c g g c t a t c g t g g c t g g c c a c g a c g g g c g t t c c t t g c g c a g c t g a c g t t c t g c t c c g t c g c g c c g a t a g c a c c g a c c g g t g c t g c c c g c a a g g a a c g c g t c g a c

Tth111IPflFI 4920 80 85 90 95 VLDVVTEAGRDWLLLGEVPG NeoR/KanR 80 85 90 95 VLDVVTEAGRDWLLLGEVPG NeoR/KanR VLDVVTEAGRDWLLLGEVPG t g c t c g a c g t t g t c a c t g a a g c g g g a a g g g a c t g g c t g c t a t t g g g c g a a g t g c c g g g g c a c g a g c t g c a a c a g t g a c t t c g c c c t t c c c t g a c c g a c g a t a a c c c g c t t c a c g g c c c c g

4980 100 105 110 115 QDLLSSHLAPAEKVSIMADA NeoR/KanR 100 105 110 115 QDLLSSHLAPAEKVSIMADA NeoR/KanR QDLLSSHLAPAEKVSIMADA *RVKSRGLFYGDHSIC a g g a t c t c c t g t c a t c t c a c c t t g c t c c t g c c g a g a a a g t a t c c a t c a t g g c t g a t g c a a t c c t a g a g g a c a g t a g a g t g g a a c g a g g a c g g c t c t t t c a t a g g t a g t a c c g a c t a c g t t

5040 120 125 130 135 MRRLHTLDPATCPFDHQAKH NeoR/KanR 120 125 130 135 MRRLHTLDPATCPFDHQAKH NeoR/KanR MRRLHTLDPATCPFDHQAKH HPPQMRKIRSGAWEVVLRFM t g c g g c g g c t g c a t a c g c t t g a t c c g g c t a c c t g c c c a t t c g a c c a c c a a g c g a a a c a t c a c g c c g c c g a c g t a t g c g a a c t a g g c c g a t g g a c g g g t a a g c t g g t g g t t c g c t t t g t a g

5100 140 145 150 155 RIERARTRMEAGLVDQDDLD NeoR/KanR 140 145 150 155 RIERARTRMEAGLVDQDDLD NeoR/KanR RIERARTRMEAGLVDQDDLD ADLSCTSPHFGTKDILIIQV g c a t c g a g c g a g c a c g t a c t c g g a t g g a a g c c g g t c t t g t c g a t c a g g a t g a t c t g g a c g c g t a g c t c g c t c g t g c a t g a g c c t a c c t t c g g c c a g a a c a g c t a g t c c t a c t a g a c c t g c

5160 160 165 170 175 EEHQGLAPAELFARLKASMP NeoR/KanR 160 165 170 175 EEHQGLAPAELFARLKASMP NeoR/KanR EEHQGLAPAELFARLKASMP FLMLPERWGFQEGPELRAHG a a g a g c a t c a g g g g c t c g c g c c a g c c g a a c t g t t c g c c a g g c t c a a g g c g a g c a t g c c c g t t c t c g t a g t c c c c g a g c g c g g t c g g c t t g a c a a g c g g t c c g a g t t c c g c t c g t a c g g g c

5220 180 185 190 195 DGEDLVVTHGDACLPNIMVE NeoR/KanR 180 185 190 195 DGEDLVVTHGDACLPNIMVE NeoR/KanR DGEDLVVTHGDACLPNIMVE VALIEDHGMAIGAQRIDHHF a c g g c g a g g a t c t c g t c g t g a c c c a t g g c g a t g c c t g c t t g c c g a a t a t c a t g g t g g a a a t g c c g c t c c t a g a g c a g c a c t g g g t a c c g c t a c g g a c g a a c g g c t t a t a g t a c c a c c t t t

RsrII 5280 200 205 210 215 NGRFSGFIDCGRLGVADRYQ NeoR/KanR 200 205 210 215 NGRFSGFIDCGRLGVADRYQ NeoR/KanR NGRFSGFIDCGRLGVADRYQ IAAKRSEDVTAPQTHRVAIL a t g g c c g c t t t t c t g g a t t c a t c g a c t g t g g c c g g c t g g g t g t g g c g g a c c g c t a t c a g g t a c c g g c g a a a a g a c c t a a g t a g c t g a c a c c g g c c g a c c c a c a c c g c c t g g c g a t a g t c c

5340 220 225 230 235 DIALATRDIAEELGGEWADR NeoR/KanR 220 225 230 235 DIALATRDIAEELGGEWADR NeoR/KanR C G T T G G C T A C C C G T G A T A T T Neo-F DIALATRDIAEELGGEWADR VYRQSGTINSFLKAAFPSVA a c a t a g c g t t g g c t a c c c g t g a t a t t g c t g a a g a g c t t g g c g g c g a a t g g g c t g a c c g c t t g t a t c g c a a c c g a t g g g c a c t a t a a c g a c t t c t c g a a c c g c c g c t t a c c c g a c t g g c g a

5400 240 245 250 255 FLVLYGIAAPDSQRIAFYRL NeoR/KanR 240 245 250 255 FLVLYGIAAPDSQRIAFYRL NeoR/KanR FLVLYGIAAPDSQRIAFYRL EEHKVTDGSGIRLADGEIAK t c c t c g t g c t t t a c g g t a t c g c c g c t c c c g a t t c g c a g c g c a t c g c c t t c t a t c g c c t t c a g g a g c a c g a a a t g c c a t a g c g g c g a g g g c t a a g c g t c g c g t a g c g g a a g a t a g c g g a a g

BstBI 5460 260 265 LDEFF* NeoR/KanR 260 265 LDEFF* NeoR/KanR MTDQATPN LDEFF* KVLEESRSEPTRFSRGLSAW t t g a c g a g t t c t t c t g a g c g g g a c t c t g g g g t t c g a a a t g a c c g a c c a a g c g a c g c c c a a a a c t g c t c a a g a a g a c t c g c c c t g a g a c c c c a a g c t t t a c t g g c t g g t t c g c t g c g g g t t

5520 LPSRDFDSTAAFYERLGFGI GAM c c t g c c a t c a c g a g a t t t c g a t t c c a c c g c c g c c t t c t a t g a a a g g t t g g g c t t c g g a a t g g a c g g t a g t g c t c t a a a g c t a a g g t g g c g g c g g a a g a t a c t t t c c a a c c c g a a g c c t t a

5580 VFRDAGWMILQRGDLMLEFF c g t t t t c c g g g a c g c c g g c t g g a t g a t c c t c c a g c g c g g g g a t c t c a t g c t g g a g t t c t t g c a a a a g g c c c t g c g g c c g a c c t a c t a g g a g g t c g c g c c c c t a g a g t a c g a c c t c a a g a a

5640 A C T T T G T G C C T T C C T C T G T T TK-pA-R AHPRGRLTETRKETIPEGTR c g c c c a c c c t a g g g g g a g g c t a a c t g a a a c a c g g a a g g a g a c a a t a c c g g a a g g a a c c c g g c g g g t g g g a t c c c c c t c c g a t t g a c t t t g t g c c t t c c t c t g t t a t g g c c t t c c t t g g g c

5700 HSV TK poly(A) signal AMTAIKRQNKTHGVGSFVHK c g c t a t g a c g g c a a t a a a a a g a c a g a a t a a a a c g c a c g g t g t t g g g t c g t t t g t t c a t a a g c g a t a c t g c c g t t a t t t t t c t g t c t t a t t t t g c g t g c c a c a a c c c a g c a a a c a a g t a t t

5760 RGVRSQGWHSVDTPPRPHWG a c g c g g g g t t c g g t c c c a g g g c t g g c a c t c t g t c g a t a c c c c a c c g a g a c c c c a t t g g g g t g c g c c c c a a g c c a g g g t c c c g a c c g t g a g a c a g c t a t g g g g t g g c t c t g g g g t a a c c c c

5820 QYARVSSFSPPHPPSSGEGP c c a a t a c g c c c g c g t t t c t t c c t t t t c c c c a c c c c a c c c c c c a a g t t c g g g t g a a g g c c c g g t t a t g c g g g c g c a a a g a a g g a a a a g g g g t g g g g t g g g g g g t t c a a g c c c a c t t c c g g g

5880 GLAANVGAAGPAIASGYSYI a g g g c t c g c a g c c a a c g t c g g g g c g g c a g g c c c t g c c a t a g c c t c a g g t t a c t c a t a t a t t c c c g a g c g t c g g t t g c a g c c c c g c c g t c c g g g a c g g t a t c g g a g t c c a a t g a g t a t a t a

5940 L* a c t t t a g a t t g a t t t a a a a c t t c a t t t t t a a t t t a a a a g g a t c t a g g t g a a g a t c c t t t t t g a a a t c t a a c t a a a t t t t g a a g t a a a a a t t a a a t t t t c c t a g a t c c a c t t c t a g g a a a a

6000 t g a t a a t c t c a t g a c c a a a a t c c c t t a a c g t g a g t t t t c g t t c c a c t g a g c g t c a g a c c c a c t a t t a g a g t a c t g g t t t t a g g g a a t t g c a c t c a a a a g c a a g g t g a c t c g c a g t c t g g g

6060 ori c g t a g a a a a g a t c a a a g g a t c t t c t t g a g a t c c t t t t t t t c t g c g c g t a a t c t g c t g c t t g c a t c t t t t c t a g t t t c c t a g a a g a a c t c t a g g a a a a a a a g a c g c g c a t t a g a c g a c g a a

6120 ori g c a a a c a a a a a a a c c a c c g c t a c c a g c g g t g g t t t g t t t g c c g g a t c a a g a g c t a c c a a c c g t t t g t t t t t t t g g t g g c g a t g g t c g c c a c c a a a c a a a c g g c c t a g t t c t c g a t g g t t g

6180 ori t c t t t t t c c g a a g g t a a c t g g c t t c a g c a g a g c g c a g a t a c c a a a t a c t g t c c t t c t a g t a g a a a a a g g c t t c c a t t g a c c g a a g t c g t c t c g c g t c t a t g g t t t a t g a c a g g a a g a t c a

6240 ori g t a g c c g t a g t t a g g c c a c c a c t t c a a g a a c t c t g t a g c a c c g c c t a c a t a c c t c g c t c t c a t c g g c a t c a a t c c g g t g g t g a a g t t c t t g a g a c a t c g t g g c g g a t g t a t g g a g c g a g a

6300 ori g c t a a t c c t g t t a c c a g t g g c t g c t g c c a g t g g c g a t a a g t c g t g t c t t a c c g g g t t g g a c g a t t a g g a c a a t g g t c a c c g a c g a c g g t c a c c g c t a t t c a g c a c a g a a t g g c c c a a c c t

ApaLI 6360 ori c t c a a g a c g a t a g t t a c c g g a t a a g g c g c a g c g g t c g g g c t g a a c g g g g g g t t c g t g c a c g a g t t c t g c t a t c a a t g g c c t a t t c c g c g t c g c c a g c c c g a c t t g c c c c c c a a g c a c g t g

6420 ori a c a g c c c a g c t t g g a g c g a a c g a c c t a c a c c g a a c t g a g a t a c c t a c a g c g t g a g c t a t g t g t c g g g t c g a a c c t c g c t t g c t g g a t g t g g c t t g a c t c t a t g g a t g t c g c a c t c g a t a c

6480 ori a g a a a g c g c c a c g c t t c c c g a a g g g a g a a a g g c g g a c a g g t a t c c g g t a a g c g g c a g g g t t c t t t c g c g g t g c g a a g g g c t t c c c t c t t t c c g c c t g t c c a t a g g c c a t t c g c c g t c c c a

6540 ori G G G A A A C G C C T G G T A T C T T T pBR322ori-F c g g a a c a g g a g a g c g c a c g a g g g a g c t t c c a g g g g g a a a c g c c t g g t a t c t t t a t a g t c c g c c t t g t c c t c t c g c g t g c t c c c t c g a a g g t c c c c c t t t g c g g a c c a t a g a a a t a t c a g g

6600 ori t g t c g g g t t t c g c c a c c t c t g a c t t g a g c g t c g a t t t t t g t g a t g c t c g t c a g g g g g g c g a c a g c c c a a a g c g g t g g a g a c t g a a c t c g c a g c t a a a a a c a c t a c g a g c a g t c c c c c c g c

6660 ori g a g c c t a t g g a a a a a c g c c a g c a a c g c g g c c t t t t t a c g g t t c c t g g c c t t t t g c t g g c c c t c g g a t a c c t t t t t g c g g t c g t t g c g c c g g a a a a a t g c c a a g g a c c g g a a a a c g a c c g g

6720 t t t t g c t c a c a t g t t c t t t c c t g c g t t a t c c c c t g a t t c t g t g g a t a a c c g t a t t a c c g c a a a a c g a g t g t a c a a g a a a g g a c g c a a t a g g g g a c t a a g a c a c c t a t t g g c a t a a t g g c g

3ʹ 5ʹ 6727 c a t g c a t g t a c g t a

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.