Addgene pAWp9 Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: pAWp9 Sequencing Result


Map View

SgrDI (11,115) AmpR promoter SspI (10,998) Amp-R (10,744 .. 10,763) PvuI (10,564) FspI (10,416) AhdI (10,194) pBR322ori-F (9442 .. 9461) PciI (9301) L4440 (9191 .. 9208) CAP binding site lac promoter M13/pUC Reverse (8973 .. 8995) lac operator M13 Reverse (8960 .. 8976) M13 rev BstZ17I (8922) EBV-rev (8881 .. 8900) SV40pA-R (8827 .. 8846) FseI (8562) SgrAI (8400) MauBI (8322) EM7 promoter SV40pro-F (8085 .. 8104) pBABE 3' (7838 .. 7858) F1ori-F (7698 .. 7719) F1ori-R (7488 .. 7507) BGH-rev (7125 .. 7142) PmeI (7109) ApaI (7104) PspOMI (7100) KpnI (6532) Acc65I (6528) pBluescriptKS (6398 .. 6414) PaeR7I - XhoI (6410) KS primer WPRE-R (5859 .. 5879) EcoRI (5782) BamHI (5764) pRS-marker (11,161 .. 11,180) BbvCI (1651) AfeI (2527) PaqCI (3364) hUBCpro-F (3520 .. 3539) PasI (3918) SfiI (4163) PshAI (4327) SbfI (4404) BstEII (5007) AgeI (5015) EGFP-N (5073 .. 5094) EXFP-R (5334 .. 5353) EGFP-C (5681 .. 5702) pAWp9 11,264 bp

Sequence View

5ʹ 3ʹ 60 a a g g c t t g a c c g a c a a t t g c a t g a a g a a t c t g c t t a g g g t t a g g c g t t t t g c g c t g c t t c t t c c g a a c t g g c t g t t a a c g t a c t t c t t a g a c g a a t c c c a a t c c g c a a a a c g c g a c g a a g

120 CMV enhancer g c g a t g t a c g g g c c a g a t a t a c g c g t t g a c a t t g a t t a t t g a c t a g t t a t t a a t a g t a a t c g c t a c a t g c c c g g t c t a t a t g c g c a a c t g t a a c t a a t a a c t g a t c a a t a a t t a t c a t t a

180 CMV enhancer c a a t t a c g g g g t c a t t a g t t c a t a g c c c a t a t a t g g a g t t c c g c g t t a c a t a a c t t a c g g g t t a a t g c c c c a g t a a t c a a g t a t c g g g t a t a t a c c t c a a g g c g c a a t g t a t t g a a t g c c

240 CMV enhancer t a a a t g g c c c g c c t g g c t g a c c g c c c a a c g a c c c c c g c c c a t t g a c g t c a a t a a t g a c g t a t t t a c c g g g c g g a c c g a c t g g c g g g t t g c t g g g g g c g g g t a a c t g c a g t t a t t a c t g c a

300 CMV enhancer a t g t t c c c a t a g t a a c g c c a a t a g g g a c t t t c c a t t g a c g t c a a t g g g t g g a g t a t t t a c t a c a a g g g t a t c a t t g c g g t t a t c c c t g a a a g g t a a c t g c a g t t a c c c a c c t c a t a a a t g

360 CMV enhancer g g t a a a c t g c c c a c t t g g c a g t a c a t c a a g t g t a t c a t a t g c c a a g t a c g c c c c c t a t t g c c a t t t g a c g g g t g a a c c g t c a t g t a g t t c a c a t a g t a t a c g g t t c a t g c g g g g g a t a a c

420 CMV enhancer a c g t c a a t g a c g g t a a a t g g c c c g c c t g g c a t t a t g c c c a g t a c a t g a c c t t a t g g g a c t t g c a g t t a c t g c c a t t t a c c g g g c g g a c c g t a a t a c g g g t c a t g t a c t g g a a t a c c c t g a

480 CMV enhancer CMV promoter t t c c t a c t t g g c a g t a c a t c t a c g t a t t a g t c a t c g c t a t t a c c a t g g t g a t g c g g t t t t a a g g a t g a a c c g t c a t g t a g a t g c a t a a t c a g t a g c g a t a a t g g t a c c a c t a c g c c a a a a

540 CMV promoter g g c a g t a c a t c a a t g g g c g t g g a t a g c g g t t t g a c t c a c g g g g a t t t c c a a g t c t c c a c c c c g t c a t g t a g t t a c c c g c a c c t a t c g c c a a a c t g a g t g c c c c t a a a g g t t c a g a g g t g g

600 CMV promoter c c a t t g a c g t c a a t g g g a g t t t g t t t t g g c a c c a a a a t c a a c g g g a c t t t c c a a a a t g t c g g t a a c t g c a g t t a c c c t c a a a c a a a a c c g t g g t t t t a g t t g c c c t g a a a g g t t t t a c a g

660 CMV promoter g t a a c a a c t c c g c c c c a t t g a c g c a a a t g g g c g g t a g g c g t g t a c g g t g g g a g g t c t a t a c a t t g t t g a g g c g g g g t a a c t g c g t t t a c c c g c c a t c c g c a c a t g c c a c c c t c c a g a t a t

720 CMV promoter 5' LTR (truncated) t a a g c a g c g c g t t t t g c c t g t a c t g g g t c t c t c t g g t t a g a c c a g a t c t g a g c c t g g g a g a t t c g t c g c g c a a a a c g g a c a t g a c c c a g a g a g a c c a a t c t g g t c t a g a c t c g g a c c c t c

780 5' LTR (truncated) c t c t c t g g c t a a c t a g g g a a c c c a c t g c t t a a g c c t c a a t a a a g c t t g c c t t g a g t g c t t g a g a g a c c g a t t g a t c c c t t g g g t g a c g a a t t c g g a g t t a t t t c g a a c g g a a c t c a c g a a

840 5' LTR (truncated) c a a g t a g t g t g t g c c c g t c t g t t g t g t g a c t c t g g t a a c t a g a g a t c c c t c a g a c c c t t t g t t c a t c a c a c a c g g g c a g a c a a c a c a c t g a g a c c a t t g a t c t c t a g g g a g t c t g g g a a a

900 5' LTR (truncated) t a g t c a g t g t g g a a a a t c t c t a g c a g t g g c g c c c g a a c a g g g a c t t g a a a g c g a a a g g g a a t c a g t c a c a c c t t t t a g a g a t c g t c a c c g c g g g c t t g t c c c t g a a c t t t c g c t t t c c c t

960 HIV-1 Ψ a a c c a g a g g a g c t c t c t c g a c g c a g g a c t c g g c t t g c t g a a g c g c g c a c g g c a a g a g g c g t t g g t c t c c t c g a g a g a g c t g c g t c c t g a g c c g a a c g a c t t c g c g c g t g c c g t t c t c c g c

1020 HIV-1 Ψ a g g g g c g g c g a c t g g t g a g t a c g c c a a a a a t t t t g a c t a g c g g a g g c t a g a a g g a g a g a g t c c c c g c c g c t g a c c a c t c a t g c g g t t t t t a a a a c t g a t c g c c t c c g a t c t t c c t c t c t c

1080 HIV-1 Ψ a t g g g t g c g a g a g c g t c a g t a t t a a g c g g g g g a g a a t t a g a t c g c g a t g g g a a a a a a t t c t a c c c a c g c t c t c g c a g t c a t a a t t c g c c c c c t c t t a a t c t a g c g c t a c c c t t t t t t a a g

1140 g g t t a a g g c c a g g g g g a a a g a a a a a a t a t a a a t t a a a a c a t a t a g t a t g g g c a a g c a g g g c c a a t t c c g g t c c c c c t t t c t t t t t t a t a t t t a a t t t t g t a t a t c a t a c c c g t t c g t c c c

1200 a g c t a g a a c g a t t c g c a g t t a a t c c t g g c c t g t t a g a a a c a t c a g a a g g c t g t a g a c a a a t c g a t c t t g c t a a g c g t c a a t t a g g a c c g g a c a a t c t t t g t a g t c t t c c g a c a t c t g t t t

1260 t a c t g g g a c a g c t a c a a c c a t c c c t t c a g a c a g g a t c a g a a g a a c t t a g a t c a t t a t a t a a t g a c c c t g t c g a t g t t g g t a g g g a a g t c t g t c c t a g t c t t c t t g a a t c t a g t a a t a t a t

1320 a t a c a g t a g c a a c c a t c t a t t g t g t g c a t c a a a g g a t a g a g a t a a a a g a c a c c a a g g a a g t a t g t c a t c g t t g g t a g a t a a c a c a c g t a g t t t c c t a t c t c t a t t t t c t g t g g t t c c t t c

1380 c t t t a g a c a a g a t a g a g g a a g a g c a a a a c a a a a g t a a g a c c a c c g c a c a g c a a g c g g c c g g a a a t c t g t t c t a t c t c c t t c t c g t t t t g t t t t c a t t c t g g t g g c g t g t c g t t c g c c g g c

1440 MRDNWRSELYK c t g a t c t t c a g a c c t g g a g g a g g a g a t a t g a g g g a c a a t t g g a g a a g t g a a t t a t a t a a a g a c t a g a a g t c t g g a c c t c c t c c t c t a t a c t c c c t g t t a a c c t c t t c a c t t a a t a t a t t t

1500 YKVVKIEPLGVAPTKAKRRV t a t a a a g t a g t a a a a a t t g a a c c a t t a g g a g t a g c a c c c a c c a a g g c a a a g a g a a g a g t g a t a t t t c a t c a t t t t t a a c t t g g t a a t c c t c a t c g t g g g t g g t t c c g t t t c t c t t c t c a c

1560 RRE VQREKRAVGIGALFLGFLGA g t g c a g a g a g a a a a a a g a g c a g t g g g a a t a g g a g c t t t g t t c c t t g g g t t c t t g g g a g c a c a c g t c t c t c t t t t t t c t c g t c a c c c t t a t c c t c g a a a c a a g g a a c c c a a g a a c c c t c g t

1620 RRE AGSTMGAASMTLTVQARQLL g c a g g a a g c a c t a t g g g c g c a g c g t c a a t g a c g c t g a c g g t a c a g g c c a g a c a a t t a t t g c g t c c t t c g t g a t a c c c g c g t c g c a g t t a c t g c g a c t g c c a t g t c c g g t c t g t t a a t a a c

BbvCI 1680 RRE SGIVQQQNNLLRAIEAQQHL t c t g g t a t a g t g c a g c a g c a g a a c a a t t t g c t g a g g g c t a t t g a g g c g c a a c a g c a t c t g a g a c c a t a t c a c g t c g t c g t c t t g t t a a a c g a c t c c c g a t a a c t c c g c g t t g t c g t a g a c

1740 RRE LQLTVWGIKQLQARILAVER t t g c a a c t c a c a g t c t g g g g c a t c a a g c a g c t c c a g g c a a g a a t c c t g g c t g t g g a a a g a a a c g t t g a g t g t c a g a c c c c g t a g t t c g t c g a g g t c c g t t c t t a g g a c c g a c a c c t t t c t

1800 RRE YLKDQQLLGIWGCSGKLICT t a c c t a a a g g a t c a a c a g c t c c t g g g g a t t t g g g g t t g c t c t g g a a a a c t c a t t t g c a c c a t g g a t t t c c t a g t t g t c g a g g a c c c c t a a a c c c c a a c g a g a c c t t t t g a g t a a a c g t g g

1860 TAVPWNASWSNKSLEQIWNH a c t g c t g t g c c t t g g a a t g c t a g t t g g a g t a a t a a a t c t c t g g a a c a g a t t t g g a a t c a c t g a c g a c a c g g a a c c t t a c g a t c a a c c t c a t t a t t t a g a g a c c t t g t c t a a a c c t t a g t g

1920 TTWMEWDREINNYTSLIHSL a c g a c c t g g a t g g a g t g g g a c a g a g a a a t t a a c a a t t a c a c a a g c t t a a t a c a c t c c t t a t g c t g g a c c t a c c t c a c c c t g t c t c t t t a a t t g t t a a t g t g t t c g a a t t a t g t g a g g a a t

1980 1 5 10 KNEQELLELDK gp41 peptide IEESQNQQEKNEQELLELDK a t t g a a g a a t c g c a a a a c c a g c a a g a a a a g a a t g a a c a a g a a t t a t t g g a a t t a g a t a a a t a a c t t c t t a g c g t t t t g g t c g t t c t t t t c t t a c t t g t t c t t a a t a a c c t t a a t c t a t t t

2040 15 WASL (in frame with gp41 peptide) WNWFNITNWLWYIKLF gp41 peptide WASLWNWFNITNWLWYIKLF t g g g c a a g t t t g t g g a a t t g g t t t a a c a t a a c a a a t t g g c t g t g g t a t a t a a a a t t a t t c a c c c g t t c a a a c a c c t t a a c c a a a t t g t a t t g t t t a a c c g a c a c c a t a t a t t t t a a t a a g

2100 (in frame with gp41 peptide) IMIVGGLVGLRIVFAVLSIV IMIVGGLVGLRIVFAVLSIV a t a a t g a t a g t a g g a g g c t t g g t a g g t t t a a g a a t a g t t t t t g c t g t a c t t t c t a t a g t g t a t t a c t a t c a t c c t c c g a a c c a t c c a a a t t c t t a t c a a a a a c g a c a t g a a a g a t a t c a c

2160 (in frame with gp41 peptide) NRVRQGYSPLSFQTHLPTPR NRVRQGYSPLSFQTHLPTPR a a t a g a g t t a g g c a g g g a t a t t c a c c a t t a t c g t t t c a g a c c c a c c t c c c a a c c c c g a g g t t a t c t c a a t c c g t c c c t a t a a g t g g t a a t a g c a a a g t c t g g g t g g a g g g t t g g g g c t c c

2220 (in frame with gp41 peptide) GPDRPEGIEEEGGERDRDRS GPDRPEGIEEEGGERDRDRS g g a c c c g a c a g g c c c g a a g g a a t a g a a g a a g a a g g t g g a g a g a g a g a c a g a g a c a g a t c c c c t g g g c t g t c c g g g c t t c c t t a t c t t c t t c t t c c a c c t c t c t c t c t g t c t c t g t c t a g g

2280 (in frame with gp41 peptide) IRLVNGSALRAPILQTNGSI IRLVNGSALRAPILQTNGSI a t t c g a t t a g t g a a c g g a t c g g c a c t g c g t g c g c c a a t t c t g c a g a c a a a t g g c a g t a t t t a a g c t a a t c a c t t g c c t a g c c g t g a c g c a c g c g g t t a a g a c g t c t g t t t a c c g t c a t a a

2340 HPQF* cPPT/CTS HPQF* c a t c c a c a a t t t t a a a a g a a a a g g g g g g a t t g g g g g g t a c a g t g c a g g g g a a a g a a t a g t g t a g g t g t t a a a a t t t t c t t t t c c c c c c t a a c c c c c c a t g t c a c g t c c c c t t t c t t a t c a

2400 cPPT/CTS a g a c a t a a t a g c a a c a g a c a t a c a a a c t a a a g a a t t a c a a a a a c a a a t t a c a a a a a t t c a t c t g t a t t a t c g t t g t c t g t a t g t t t g a t t t c t t a a t g t t t t t g t t t a a t g t t t t t a a g t

2460 cPPT/CTS a a a t t t t c g g g t t t a t t a c a g g g a c a g c a g a g a t c c a g t t t g g t t a a t t a a c c c g t g t c g t t t a a a a g c c c a a a t a a t g t c c c t g t c g t c t c t a g g t c a a a c c a a t t a a t t g g g c a c a g c

2520 UbC promoter g c t c c a g a t c t g g c c t c c g c g c c g g g t t t t g g c g c c t c c c g c g g g c g c c c c c c t c c t c a c c g a g g t c t a g a c c g g a g g c g c g g c c c a a a a c c g c g g a g g g c g c c c g c g g g g g g a g g a g t g

AfeI 2580 UbC promoter g g c g a g c g c t g c c a c g t c a g a c g a a g g g c g c a g c g a g c g t c c t g a t c c t t c c g c c c g g a c c c g c t c g c g a c g g t g c a g t c t g c t t c c c g c g t c g c t c g c a g g a c t a g g a a g g c g g g c c t g

2640 UbC promoter g c t c a g g a c a g c g g c c c g c t g c t c a t a a g a c t c g g c c t t a g a a c c c c a g t a t c a g c a g a a c g a g t c c t g t c g c c g g g c g a c g a g t a t t c t g a g c c g g a a t c t t g g g g t c a t a g t c g t c t t

2700 UbC promoter g g a c a t t t t a g g a c g g g a c t t g g g t g a c t c t a g g g c a c t g g t t t t c t t t c c a g a g a g c g g c c t g t a a a a t c c t g c c c t g a a c c c a c t g a g a t c c c g t g a c c a a a a g a a a g g t c t c t c g c c

2760 UbC promoter a a c a g g c g a g g a a a a g t a g t c c c t t c t c g g c g a t t c t g c g g a g g g a t c t c c g t g g g g c g g t t g t c c g c t c c t t t t c a t c a g g g a a g a g c c g c t a a g a c g c c t c c c t a g a g g c a c c c c g c c

2820 UbC promoter t g a a c g c c g a t g a t t a t a t a a g g a c g c g c c g g g t g t g g c a c a g c t a g t t c c g t c g c a g c c a c t t g c g g c t a c t a a t a t a t t c c t g c g c g g c c c a c a c c g t g t c g a t c a a g g c a g c g t c g g

2880 UbC promoter g g g a t t t g g g t c g c g g t t c t t g t t t g t g g a t c g c t g t g a t c g t c a c t t g g t g a g t a g c g g c c c t a a a c c c a g c g c c a a g a a c a a a c a c c t a g c g a c a c t a g c a g t g a a c c a c t c a t c g c c

2940 UbC promoter g c t g c t g g g c t g g c c g g g g c t t t c g t g g c c g c c g g g c c g c t c g g t g g g a c g g a a g c g t g t c g a c g a c c c g a c c g g c c c c g a a a g c a c c g g c g g c c c g g c g a g c c a c c c t g c c t t c g c a c a

3000 UbC promoter g g a g a g a c c g c c a a g g g c t g t a g t c t g g g t c c g c g a g c a a g g t t g c c c t g a a c t g g g g g t c c t c t c t g g c g g t t c c c g a c a t c a g a c c c a g g c g c t c g t t c c a a c g g g a c t t g a c c c c c a

3060 UbC promoter t g g g g g g a g c g c a g c a a a a t g g c g g c t g t t c c c g a g t c t t g a a t g g a a g a c g c t t g t g a g a c c c c c c t c g c g t c g t t t t a c c g c c g a c a a g g g c t c a g a a c t t a c c t t c t g c g a a c a c t c

3120 UbC promoter g c g g g c t g t g a g g t c g t t g a a a c a a g g t g g g g g g c a t g g t g g g c g g c a a g a a c c c a a g g t c g c c c g a c a c t c c a g c a a c t t t g t t c c a c c c c c c g t a c c a c c c g c c g t t c t t g g g t t c c a

3180 UbC promoter c t t g a g g c c t t c g c t a a t g c g g g a a a g c t c t t a t t c g g g t g a g a t g g g c t g g g g c a c c a t g a a c t c c g g a a g c g a t t a c g c c c t t t c g a g a a t a a g c c c a c t c t a c c c g a c c c c g t g g t a

3240 UbC promoter c t g g g g a c c c t g a c g t g a a g t t t g t c a c t g a c t g g a g a a c t c g g t t t g t c g t c t g t t g c g g a c c c c t g g g a c t g c a c t t c a a a c a g t g a c t g a c c t c t t g a g c c a a a c a g c a g a c a a c g c

3300 UbC promoter g g g g c g g c a g t t a t g g c g g t g c c g t t g g g c a g t g c a c c c g t a c c t t t g g g a g c g c g c g c c c c c c g c c g t c a a t a c c g c c a c g g c a a c c c g t c a c g t g g g c a t g g a a a c c c t c g c g c g c g g

3360 UbC promoter c t c g t c g t g t c g t g a c g t c a c c c g t t c t g t t g g c t t a t a a t g c a g g g t g g g g c c a c c t g c g a g c a g c a c a g c a c t g c a g t g g g c a a g a c a a c c g a a t a t t a c g t c c c a c c c c g g t g g a c g

PaqCI 3420 UbC promoter c g g t a g g t g t g c g g t a g g c t t t t c t c c g t c g c a g g a c g c a g g g t t c g g g c c t a g g g t a g g g c c a t c c a c a c g c c a t c c g a a a a g a g g c a g c g t c c t g c g t c c c a a g c c c g g a t c c c a t c c

3480 UbC promoter c t c t c c t g a a t c g a c a g g c g c c g g a c c t c t g g t g a g g g g a g g g a t a a g t g a g g c g t c a g t g a g a g g a c t t a g c t g t c c g c g g c c t g g a g a c c a c t c c c c t c c c t a t t c a c t c c g c a g t c a

3540 UbC promoter T G A A G C T C C G G T T T T G A A C T hUBCpro-F t t c t t t g g t c g g t t t t a t g t a c c t a t c t t c t t a a g t a g c t g a a g c t c c g g t t t t g a a c t a a a g a a a c c a g c c a a a a t a c a t g g a t a g a a g a a t t c a t c g a c t t c g a g g c c a a a a c t t g a t

3600 UbC promoter t g c g c t c g g g g t t g g c g a g t g t g t t t t g t g a a g t t t t t t a g g c a c c t t t t g a a a t g t a a t a c g c g a g c c c c a a c c g c t c a c a c a a a a c a c t t c a a a a a a t c c g t g g a a a a c t t t a c a t t a

3660 UbC promoter c a t t t g g g t c a a t a t g t a a t t t t c a g t g t t a g a c t a g t a a a t t g t c c g c t a a a t t c t g g c g t a a a c c c a g t t a t a c a t t a a a a g t c a c a a t c t g a t c a t t t a a c a g g c g a t t t a a g a c c g

3720 1 5 MSELIKE UbC promoter TurboRFP MSELIKE c g t t t t t g g c t t t t t t g t t a g a c a g t t a a t t a a g c c a c c a t g a g c g a g c t g a t c a a g g a g g c a a a a a c c g a a a a a a c a a t c t g t c a a t t a a t t c g g t g g t a c t c g c t c g a c t a g t t c c t c

3780 10 15 20 25 NMHMKLYMEGTVNNHHFKCT TurboRFP NMHMKLYMEGTVNNHHFKCT a a c a t g c a c a t g a a g c t g t a c a t g g a g g g c a c c g t g a a c a a c c a c c a c t t c a a g t g c a c a t t g t a c g t g t a c t t c g a c a t g t a c c t c c c g t g g c a c t t g t t g g t g g t g a a g t t c a c g t g t

3840 30 35 40 45 SEGEGKPYEGTQTMKIKVVE TurboRFP SEGEGKPYEGTQTMKIKVVE t c c g a g g g c g a a g g c a a g c c c t a c g a g g g c a c c c a g a c c a t g a a g a t c a a g g t g g t c g a g a g g c t c c c g c t t c c g t t c g g g a t g c t c c c g t g g g t c t g g t a c t t c t a g t t c c a c c a g c t c

3900 50 55 60 65 GGPLPFAFDILATSFMYGSK TurboRFP GGPLPFAFDILATSFMYGSK g g c g g c c c t c t c c c c t t c g c c t t c g a c a t c c t g g c t a c c a g c t t c a t g t a c g g c a g c a a a c c g c c g g g a g a g g g g a a g c g g a a g c t g t a g g a c c g a t g g t c g a a g t a c a t g c c g t c g t t t

PasI 3960 70 75 80 85 AFINHTQGIPDFFKQSFPEG TurboRFP AFINHTQGIPDFFKQSFPEG g c c t t c a t c a a c c a c a c c c a g g g c a t c c c c g a c t t c t t t a a g c a g t c c t t c c c t g a g g g c c g g a a g t a g t t g g t g t g g g t c c c g t a g g g g c t g a a g a a a t t c g t c a g g a a g g g a c t c c c g

4020 90 95 100 105 FTWERITTYEDGGVLTATQD TurboRFP FTWERITTYEDGGVLTATQD t t c a c a t g g g a g a g a a t c a c c a c a t a c g a a g a c g g g g g c g t g c t g a c c g c t a c c c a g g a c a a g t g t a c c c t c t c t t a g t g g t g t a t g c t t c t g c c c c c g c a c g a c t g g c g a t g g g t c c t g

4080 110 115 120 125 TSFQNGCIIYNVKINGVNFP TurboRFP TSFQNGCIIYNVKINGVNFP a c c a g c t t c c a g a a c g g c t g c a t c a t c t a c a a c g t c a a g a t c a a c g g g g t g a a c t t c c c a t g g t c g a a g g t c t t g c c g a c g t a g t a g a t g t t g c a g t t c t a g t t g c c c c a c t t g a a g g g t

4140 130 135 140 145 SNGPVMQKKTRGWEANTEML TurboRFP SNGPVMQKKTRGWEANTEML t c c a a c g g c c c t g t g a t g c a g a a g a a a a c a c g c g g c t g g g a g g c c a a c a c c g a g a t g c t g a g g t t g c c g g g a c a c t a c g t c t t c t t t t g t g c g c c g a c c c t c c g g t t g t g g c t c t a c g a c

SfiI 4200 150 155 160 165 YPADGGLRGHSQMALKLVGG TurboRFP YPADGGLRGHSQMALKLVGG t a c c c c g c t g a c g g c g g c c t g a g a g g c c a c a g c c a g a t g g c c c t g a a g c t c g t g g g c g g g a t g g g g c g a c t g c c g c c g g a c t c t c c g g t g t c g g t c t a c c g g g a c t t c g a g c a c c c g c c c

4260 170 175 180 185 GYLHCSFKTTYRSKKPAKNL TurboRFP GYLHCSFKTTYRSKKPAKNL g g c t a c c t g c a c t g c t c c t t c a a g a c c a c a t a c a g a t c c a a g a a a c c c g c t a a g a a c c t c c c g a t g g a c g t g a c g a g g a a g t t c t g g t g t a t g t c t a g g t t c t t t g g g c g a t t c t t g g a g

4320 190 195 200 205 KMPGFHFVDHRLERIKEADK TurboRFP KMPGFHFVDHRLERIKEADK a a g a t g c c c g g c t t c c a c t t c g t g g a c c a c a g a c t g g a a a g a a t c a a g g a g g c c g a c a a a t t c t a c g g g c c g a a g g t g a a g c a c c t g g t g t c t g a c c t t t c t t a g t t c c t c c g g c t g t t t

PshAI 4380 210 215 220 225 ETYVEQHEMAVAKYCDLPSK TurboRFP ETYVEQHEMAVAKYCDLPSK g a g a c c t a c g t c g a g c a g c a c g a g a t g g c t g t g g c c a a g t a c t g c g a c c t c c c t a g c a a a c t c t g g a t g c a g c t c g t c g t g c t c t a c c g a c a c c g g t t c a t g a c g c t g g a g g g a t c g t t t

SbfI 4440 230 LGHR* TurboRFP CMV enhancer LGHR* c t g g g g c a c a g a t a a t a a c c t g c a g g c g g g c c a g a t a t a c g c g t t g a c a t t g a t t a t t g a g a c c c c g t g t c t a t t a t t g g a c g t c c g c c c g g t c t a t a t g c g c a a c t g t a a c t a a t a a c t

4500 CMV enhancer c t a g t t a t t a a t a g t a a t c a a t t a c g g g g t c a t t a g t t c a t a g c c c a t a t a t g g a g t t c c g a t c a a t a a t t a t c a t t a g t t a a t g c c c c a g t a a t c a a g t a t c g g g t a t a t a c c t c a a g g

4560 CMV enhancer g c g t t a c a t a a c t t a c g g t a a a t g g c c c g c c t g g c t g a c c g c c c a a c g a c c c c c g c c c a t c g c a a t g t a t t g a a t g c c a t t t a c c g g g c g g a c c g a c t g g c g g g t t g c t g g g g g c g g g t a

4620 CMV enhancer t g a c g t c a a t a a t g a c g t a t g t t c c c a t a g t a a c g c c a a t a g g g a c t t t c c a t t g a c g t c a c t g c a g t t a t t a c t g c a t a c a a g g g t a t c a t t g c g g t t a t c c c t g a a a g g t a a c t g c a g

4680 CMV enhancer a a t g g g t g g a g t a t t t a c g g t a a a c t g c c c a c t t g g c a g t a c a t c a a g t g t a t c a t a t g c t t a c c c a c c t c a t a a a t g c c a t t t g a c g g g t g a a c c g t c a t g t a g t t c a c a t a g t a t a c g

4740 CMV enhancer c a a g t a c g c c c c c t a t t g a c g t c a a t g a c g g t a a a t g g c c c g c c t g g c a t t a t g c c c a g t g t t c a t g c g g g g g a t a a c t g c a g t t a c t g c c a t t t a c c g g g c g g a c c g t a a t a c g g g t c a

4800 CMV enhancer a c a t g a c c t t a t g g g a c t t t c c t a c t t g g c a g t a c a t c t a c g t a t t a g t c a t c g c t a t t a t g t a c t g g a a t a c c c t g a a a g g a t g a a c c g t c a t g t a g a t g c a t a a t c a g t a g c g a t a a t

4860 CMV enhancer CMV promoter c c a t g g t g a t g c g g t t t t g g c a g t a c a t c a a t g g g c g t g g a t a g c g g t t t g a c t c a c g g g g g t a c c a c t a c g c c a a a a c c g t c a t g t a g t t a c c c g c a c c t a t c g c c a a a c t g a g t g c c c

4920 CMV promoter g a t t t c c a a g t c t c c a c c c c a t t g a c g t c a a t g g g a g t t t g t t t t g g c a c c a a a a t c a a c c t a a a g g t t c a g a g g t g g g g t a a c t g c a g t t a c c c t c a a a c a a a a c c g t g g t t t t a g t t g

4980 CMV promoter MGGRR g g g a c t t t c c a a a a t g t c g t a a c a a c t c c g c c c c a t t g a c g c a a a t g g g c g g t a g g c g t g c c c t g a a a g g t t t t a c a g c a t t g t t g a g g c g g g g t a a c t g c g t t t a c c c g c c a t c c g c a c

AgeIBstEII 5040 1 M 1a V SK CMV promoter EGFP 1 M 1a V SK EGFP Kozak sequence VRWEVYISSGNLPVATMVSK t a c g g t g g g a g g t c t a t a t a a g c a g c g g t a a c c t a c c g g t c g c c a c c a t g g t g a g c a a g g a t g c c a c c c t c c a g a t a t a t t c g t c g c c a t t g g a t g g c c a g c g g t g g t a c c a c t c g t t c c

5100 5 10 15 20 GEELFTGVVPILVELDGDVN EGFP 5 10 15 20 GEELFTGVVPILVELDGDVN EGFP G A C C A G C T C G A C C T G C C G C T G C EGFP-N GEELFTGVVPILVELDGDVN g c g a g g a g c t g t t c a c c g g g g t g g t g c c c a t c c t g g t c g a g c t g g a c g g c g a c g t a a a c g c g c t c c t c g a c a a g t g g c c c c a c c a c g g g t a g g a c c a g c t c g a c c t g c c g c t g c a t t t g c

5160 25 30 35 40 GHKFSVSGEGEGDATYGKLT EGFP 25 30 35 40 GHKFSVSGEGEGDATYGKLT EGFP GHKFSVSGEGEGDATYGKLT g c c a c a a g t t c a g c g t g t c c g g c g a g g g c g a g g g c g a t g c c a c c t a c g g c a a g c t g a c c c c g g t g t t c a a g t c g c a c a g g c c g c t c c c g c t c c c g c t a c g g t g g a t g c c g t t c g a c t g g g

5220 45 50 55 60 LKFICTTGKLPVPWPTLVTT EGFP 45 50 55 60 LKFICTTGKLPVPWPTLVTT EGFP LKFICTTGKLPVPWPTLVTT t g a a g t t c a t c t g c a c c a c c g g c a a g c t g c c c g t g c c c t g g c c c a c c c t c g t g a c c a c c c a c t t c a a g t a g a c g t g g t g g c c g t t c g a c g g g c a c g g g a c c g g g t g g g a g c a c t g g t g g g

5280 65 70 75 80 LTYGVQCFSRYPDHMKQHDF EGFP 65 70 75 80 LTYGVQCFSRYPDHMKQHDF EGFP LTYGVQCFSRYPDHMKQHDF t g a c c t a c g g c g t g c a g t g c t t c a g c c g c t a c c c c g a c c a c a t g a a g c a g c a c g a c t t c t a c t g g a t g c c g c a c g t c a c g a a g t c g g c g a t g g g g c t g g t g t a c t t c g t c g t g c t g a a g a

5340 85 90 95 100 FKSAMPEGYVQERTIFFKDD EGFP 85 90 95 100 FKSAMPEGYVQERTIFFKDD EGFP C T G C T G C EXFP-R FKSAMPEGYVQERTIFFKDD t c a a g t c c g c c a t g c c c g a a g g c t a c g t c c a g g a g c g c a c c a t c t t c t t c a a g g a c g a c g a g t t c a g g c g g t a c g g g c t t c c g a t g c a g g t c c t c g c g t g g t a g a a g a a g t t c c t g c t g c

5400 105 110 115 120 GNYKTRAEVKFEGDTLVNRI EGFP 105 110 115 120 GNYKTRAEVKFEGDTLVNRI EGFP C G T T G A T G T T C T G EXFP-R GNYKTRAEVKFEGDTLVNRI g c a a c t a c a a g a c c c g c g c c g a g g t g a a g t t c g a g g g c g a c a c c c t g g t g a a c c g c a t c g c g t t g a t g t t c t g g g c g c g g c t c c a c t t c a a g c t c c c g c t g t g g g a c c a c t t g g c g t a g c

5460 125 130 135 140 ELKGIDFKEDGNILGHKLEY EGFP 125 130 135 140 ELKGIDFKEDGNILGHKLEY EGFP ELKGIDFKEDGNILGHKLEY a g c t g a a g g g c a t c g a c t t c a a g g a g g a c g g c a a c a t c c t g g g g c a c a a g c t g g a g t a c a t c g a c t t c c c g t a g c t g a a g t t c c t c c t g c c g t t g t a g g a c c c c g t g t t c g a c c t c a t g t

5520 145 150 155 160 NYNSHNVYIMADKQKNGIKV EGFP 145 150 155 160 NYNSHNVYIMADKQKNGIKV EGFP NYNSHNVYIMADKQKNGIKV a c t a c a a c a g c c a c a a c g t c t a t a t c a t g g c c g a c a a g c a g a a g a a c g g c a t c a a g g t g a t g a t g t t g t c g g t g t t g c a g a t a t a g t a c c g g c t g t t c g t c t t c t t g c c g t a g t t c c a c t

5580 165 170 175 180 NFKIRHNIEDGSVQLADHYQ EGFP 165 170 175 180 NFKIRHNIEDGSVQLADHYQ EGFP NFKIRHNIEDGSVQLADHYQ a c t t c a a g a t c c g c c a c a a c a t c g a g g a c g g c a g c g t g c a g c t c g c c g a c c a c t a c c a g c t g a a g t t c t a g g c g g t g t t g t a g c t c c t g c c g t c g c a c g t c g a g c g g c t g g t g a t g g t c g

5640 185 190 195 200 QNTPIGDGPVLLPDNHYLST EGFP 185 190 195 200 QNTPIGDGPVLLPDNHYLST EGFP QNTPIGDGPVLLPDNHYLST a g a a c a c c c c c a t c g g c g a c g g c c c c g t g c t g c t g c c c g a c a a c c a c t a c c t g a g c a c c c t c t t g t g g g g g t a g c c g c t g c c g g g g c a c g a c g a c g g g c t g t t g g t g a t g g a c t c g t g g g

5700 205 210 215 220 QSALSKDPNEKRDHMVLLEF EGFP 205 210 215 220 QSALSKDPNEKRDHMVLLEF EGFP C A T G G T C C T G C T G G A G T T C G EGFP-C QSALSKDPNEKRDHMVLLEF a g t c c g c c c t g a g c a a a g a c c c c a a c g a g a a g c g c g a t c a c a t g g t c c t g c t g g a g t t c g t c a g g c g g g a c t c g t t t c t g g g g t t g c t c t t c g c g c t a g t g t a c c a g g a c g a c c t c a a g c

5760 225 230 235 VTAAGITLGMDELYK* EGFP 225 230 235 VTAAGITLGMDELYK * EGFP T G EGFP-C VTAAGITLGMDELYK* t g a c c g c c g c c g g g a t c a c t c t c g g c a t g g a c g a g c t g t a c a a g t a a a g c g g c c g c g a c t a c t g g c g g c g g c c c t a g t g a g a g c c g t a c c t g c t c g a c a t g t t c a t t t c g c c g g c g c t g a

EcoRIBamHI 5820 WPRE c t a g g a t c c g c g a a a a g t c c g g a a t t c g a t a t c a a g c t t a t c g a t a a t c a a c c t c t g g a t g a t c c t a g g c g c t t t t c a g g c c t t a a g c t a t a g t t c g a a t a g c t a t t a g t t g g a g a c c t a

5880 WPRE A C A A C G A G G A A A A T G C G A T A C WPRE-R t a c a a a a t t t g t g a a a g a t t g a c t g g t a t t c t t a a c t a t g t t g c t c c t t t t a c g c t a t g t a t g t t t t a a a c a c t t t c t a a c t g a c c a t a a g a a t t g a t a c a a c g a g g a a a a t g c g a t a c a

5940 WPRE MPLYHAIASRMAFIF MLLLPVWLSFS g g a t a c g c t g c t t t a a t g c c t t t g t a t c a t g c t a t t g c t t c c c g t a t g g c t t t c a t t t t c c c t a t g c g a c g a a a t t a c g g a a a c a t a g t a c g a t a a c g a a g g g c a t a c c g a a a g t a a a a g

6000 WPRE SSLYKSWLLSLYEELWPVVR PPCINPGCCLFMRSCGPLSG t c c t c c t t g t a t a a a t c c t g g t t g c t g t c t c t t t a t g a g g a g t t g t g g c c c g t t g t c a g g a g g a g g a a c a t a t t t a g g a c c a a c g a c a g a g a a a t a c t c c t c a a c a c c g g g c a a c a g t c c

6060 WPRE QRGVVCTVFADATPTGWGIA NVAWCALCLLTQPPLVGALP c a a c g t g g c g t g g t g t g c a c t g t g t t t g c t g a c g c a a c c c c c a c t g g t t g g g g c a t t g c c g t t g c a c c g c a c c a c a c g t g a c a c a a a c g a c t g c g t t g g g g g t g a c c a a c c c c g t a a c g g

6120 WPRE TTCQLLSGTFAFPLPIATAE PPVSSFPGLSLSPSLLPRRN a c c a c c t g t c a g c t c c t t t c c g g g a c t t t c g c t t t c c c c c t c c c t a t t g c c a c g g c g g a a t g g t g g a c a g t c g a g g a a a g g c c c t g a a a g c g a a a g g g g g a g g g a t a a c g g t g c c g c c t t

6180 WPRE LIAACLARCWTGARLLGTDN SSPPALPAAGQGLGCWALTI c t c a t c g c c g c c t g c c t t g c c c g c t g c t g g a c a g g g g c t c g g c t g t t g g g c a c t g a c a a t g a g t a g c g g c g g a c g g a a c g g g c g a c g a c c t g t c c c c g a g c c g a c a a c c c g t g a c t g t t a

6240 WPRE (in frame with Factor Xa site) *RGKRPQEGTNGG SVVLSGKSSSFPWLLACVAT PWCCRGNHRPFLGCSPVLPP t c c g t g g t g t t g t c g g g g a a a t c a t c g t c c t t t c c t t g g c t g c t c g c c t g t g t t g c c a c c a g g c a c c a c a a c a g c c c c t t t a g t a g c a g g a a a g g a a c c g a c g a g c g g a c a c a a c g g t g g

6300 WPRE (in frame with Factor Xa site) PNQAPRGEAVDR 1 RGEI Factor Xa site WILRGTSFCYVPSALNPADL GFCAGRPSATSLRPSIQRTF t g g a t t c t g c g c g g g a c g t c c t t c t g c t a c g t c c c t t c g g c c c t c a a t c c a g c g g a c c t t a c c t a a g a c g c g c c c t g c a g g a a g a c g a t g c a g g g a a g c c g g g a g t t a g g t c g c c t g g a a

6360 WPRE PSRGLLPALRPLPRLRLRPQ LPAACCRLCGLFRVFAFALR c c t t c c c g c g g c c t g c t g c c g g c t c t g c g g c c t c t t c c g c g t c t t c g c c t t c g c c c t c a g g g a a g g g c g c c g g a c g a c g g c c g a g a c g c c g g a g a a g g c g c a g a a g c g g a a g c g g g a g t c

PaeR7IXhoI 6420 WPRE KS primer C T A T G G C A G C T G G A G C T pBluescriptKS TSRISLWAASPHRYRRPRDL RVGSPFGPPPRIDTVDLET* a c g a g t c g g a t c t c c c t t t g g g c c g c c t c c c c g c a t c g a t a c c g t c g a c c t c g a g a c c t a t g c t c a g c c t a g a g g g a a a c c c g g c g g a g g g g c g t a g c t a t g g c a g c t g g a g c t c t g g a t

6480 EKHGAITSSNTAATNADCAW g a a a a a c a t g g a g c a a t c a c a a g t a g c a a t a c a g c a g c t a c c a a t g c t g a t t g t g c c t g g c t t t t t g t a c c t c g t t a g t g t t c a t c g t t a t g t c g t c g a t g g t t a c g a c t a a c a c g g a c c

Acc65IKpnI 6540 LEAQEEEEVGFPVTPQVPLR c t a g a a g c a c a a g a g g a g g a g g a g g t g g g t t t t c c a g t c a c a c c t c a g g t a c c t t t a a g a g a t c t t c g t g t t c t c c t c c t c c t c c a c c c a a a a g g t c a g t g t g g a g t c c a t g g a a a t t c t

6600 PMTYKAAVDLSHFLKEKGGL c c a a t g a c t t a c a a g g c a g c t g t a g a t c t t a g c c a c t t t t t a a a a g a a a a g g g g g g a c t g g g t t a c t g a a t g t t c c g t c g a c a t c t a g a a t c g g t g a a a a a t t t t c t t t t c c c c c c t g a c

6660 EGLIHSQRRQDILDLWIYHT g a a g g g c t a a t t c a c t c c c a a c g a a g a c a a g a t a t c c t t g a t c t g t g g a t c t a c c a c a c a c t t c c c g a t t a a g t g a g g g t t g c t t c t g t t c t a t a g g a a c t a g a c a c c t a g a t g g t g t g t

6720 QGYFPDWQNYTPGPGIRYPL c a a g g c t a c t t c c c t g a t t g g c a g a a c t a c a c a c c a g g g c c a g g g a t c a g a t a t c c a c t g g t t c c g a t g a a g g g a c t a a c c g t c t t g a t g t g t g g t c c c g g t c c c t a g t c t a t a g g t g a c

6780 TFGWCYKLVPVEQEKVEEAN a c c t t t g g a t g g t g c t a c a a g c t a g t a c c a g t t g a g c a a g a g a a g g t a g a a g a a g c c a a t t g g a a a c c t a c c a c g a t g t t c g a t c a t g g t c a a c t c g t t c t c t t c c a t c t t c t t c g g t t a

6840 EGENTRLLHPVSLHGMDDPE g a a g g a g a g a a c a c c c g c t t g t t a c a c c c t g t g a g c c t g c a t g g g a t g g a t g a c c c g g a g c t t c c t c t c t t g t g g g c g a a c a a t g t g g g a c a c t c g g a c g t a c c c t a c c t a c t g g g c c t c

6900 REVLEWRFDSRLAFHHMARE a g a g a a g t a t t a g a g t g g a g g t t t g a c a g c c g c c t a g c a t t t c a t c a c a t g g c c c g a g a g t c t c t t c a t a a t c t c a c c t c c a a a c t g t c g g c g g a t c g t a a a g t a g t g t a c c g g g c t c t c

6960 5' LTR (truncated) LHPDCTGSLWLDQI* c t g c a t c c g g a c t g t a c t g g g t c t c t c t g g t t a g a c c a g a t c t g a g c c t g g g a g c t c t c t g a c g t a g g c c t g a c a t g a c c c a g a g a g a c c a a t c t g g t c t a g a c t c g g a c c c t c g a g a g a

7020 5' LTR (truncated) g g c t a a c t a g g g a a c c c a c t g c t t a a g c c t c a a t a a a g c t t g c c t t g a g t g c t t c a a g t a c c g a t t g a t c c c t t g g g t g a c g a a t t c g g a g t t a t t t c g a a c g g a a c t c a c g a a g t t c a t

7080 5' LTR (truncated) g t g t g t g c c c g t c t g t t g t g t g a c t c t g g t a a c t a g a g a t c c c t c a g a c c c t t t t a g t c a c a c a c a c g g g c a g a c a a c a c a c t g a g a c c a t t g a t c t c t a g g g a g t c t g g g a a a a t c a g t

PmeIPspOMIApaI 7140 5' LTR (truncated) bGH poly(A) signal G G A G C T G A C A C G G A A G BGH-rev g t g t g g a a a a t c t c t a g c a g g g c c c g t t t a a a c c c g c t g a t c a g c c t c g a c t g t g c c t t c c a c a c c t t t t a g a g a t c g t c c c g g g c a a a t t t g g g c g a c t a g t c g g a g c t g a c a c g g a a g

7200 bGH poly(A) signal A T BGH-rev t a g t t g c c a g c c a t c t g t t g t t t g c c c c t c c c c c g t g c c t t c c t t g a c c c t g g a a g g t g c a t c a a c g g t c g g t a g a c a a c a a a c g g g g a g g g g g c a c g g a a g g a a c t g g g a c c t t c c a c g

7260 bGH poly(A) signal c a c t c c c a c t g t c c t t t c c t a a t a a a a t g a g g a a a t t g c a t c g c a t t g t c t g a g t a g g t g g t g a g g g t g a c a g g a a a g g a t t a t t t t a c t c c t t t a a c g t a g c g t a a c a g a c t c a t c c a c

7320 bGH poly(A) signal t c a t t c t a t t c t g g g g g g t g g g g t g g g g c a g g a c a g c a a g g g g g a g g a t t g g g a a g a c a a a g t a a g a t a a g a c c c c c c a c c c c a c c c c g t c c t g t c g t t c c c c c t c c t a a c c c t t c t g t t

7380 bGH poly(A) signal t a g c a g g c a t g c t g g g g a t g c g g t g g g c t c t a t g g c t t c t g a g g c g g a a a g a a c c a g c t g a t c g t c c g t a c g a c c c c t a c g c c a c c c g a g a t a c c g a a g a c t c c g c c t t t c t t g g t c g a c

7440 f1 ori g g g c t c t a g g g g g t a t c c c c a c g c g c c c t g t a g c g g c g c a t t a a g c g c g g c g g g t g t g g t c c c g a g a t c c c c c a t a g g g g t g c g c g g g a c a t c g c c g c g t a a t t c g c g c c g c c c a c a c c a

7500 f1 ori G A G G A A A G C G A A A F1ori-R g g t t a c g c g c a g c g t g a c c g c t a c a c t t g c c a g c g c c c t a g c g c c c g c t c c t t t c g c t t t c c a a t g c g c g t c g c a c t g g c g a t g t g a a c g g t c g c g g g a t c g c g g g c g a g g a a a g c g a a a

7560 f1 ori G A A G G G A F1ori-R c t t c c c t t c c t t t c t c g c c a c g t t c g c c g g c t t t c c c c g t c a a g c t c t a a a t c g g g g g c t g a a g g g a a g g a a a g a g c g g t g c a a g c g g c c g a a a g g g g c a g t t c g a g a t t t a g c c c c c g a

7620 f1 ori c c c t t t a g g g t t c c g a t t t a g t g c t t t a c g g c a c c t c g a c c c c a a a a a a c t t g a t t a g g g g g g a a a t c c c a a g g c t a a a t c a c g a a a t g c c g t g g a g c t g g g g t t t t t t g a a c t a a t c c c

7680 f1 ori t g a t g g t t c a c g t a g t g g g c c a t c g c c c t g a t a g a c g g t t t t t c g c c c t t t g a c g t t g g a a c t a c c a a g t g c a t c a c c c g g t a g c g g g a c t a t c t g c c a a a a a g c g g g a a a c t g c a a c c t

7740 f1 ori G T G G A C T C T T G T T C C A A A C T G G F1ori-F g t c c a c g t t c t t t a a t a g t g g a c t c t t g t t c c a a a c t g g a a c a a c a c t c a a c c c t a t c t c c a g g t g c a a g a a a t t a t c a c c t g a g a a c a a g g t t t g a c c t t g t t g t g a g t t g g g a t a g a g

7800 f1 ori g g t c t a t t c t t t t g a t t t a t a a g g g a t t t t g c c g a t t t c g g c c t a t t g g t t a a a a a a t g a c c a g a t a a g a a a a c t a a a t a t t c c c t a a a a c g g c t a a a g c c g g a t a a c c a a t t t t t t a c t

7860 f1 ori SV40 promoter C C T T A C A C A C A G T C A A T C C C A pBABE 3' g c t g a t t t a a c a a a a a t t t a a c g c g a a t t a a t t c t g t g g a a t g t g t g t c a g t t a g g g t g t c g a c t a a a t t g t t t t t a a a t t g c g c t t a a t t a a g a c a c c t t a c a c a c a g t c a a t c c c a c a

7920 SV40 promoter g g a a a g t c c c c a g g c t c c c c a g c a g g c a g a a g t a t g c a a a g c a t g c a t c t c a a t t a g t c a c c t t t c a g g g g t c c g a g g g g t c g t c c g t c t t c a t a c g t t t c g t a c g t a g a g t t a a t c a g t

7980 SV40 promoter g c a a c c a g g t g t g g a a a g t c c c c a g g c t c c c c a g c a g g c a g a a g t a t g c a a a g c a t g c a t c g t t g g t c c a c a c c t t t c a g g g g t c c g a g g g g t c g t c c g t c t t c a t a c g t t t c g t a c g t a

8040 SV40 promoter SV40 ori c t c a a t t a g t c a g c a a c c a t a g t c c c g c c c c t a a c t c c g c c c a t c c c g c c c c t a a c t c c g g a g t t a a t c a g t c g t t g g t a t c a g g g c g g g g a t t g a g g c g g g t a g g g c g g g g a t t g a g g c

8100 SV40 promoter SV40 ori T A T T T A T G C A G A G G C C SV40pro-F c c c a g t t c c g c c c a t t c t c c g c c c c a t g g c t g a c t a a t t t t t t t t a t t t a t g c a g a g g c c g g g t c a a g g c g g g t a a g a g g c g g g g t a c c g a c t g a t t a a a a a a a a t a a a t a c g t c t c c g g

8160 SV40 promoter SV40 ori G A G G SV40pro-F g a g g c c g c c t c t g c c t c t g a g c t a t t c c a g a a g t a g t g a g g a g g c t t t t t t g g a g g c c t a c t c c g g c g g a g a c g g a g a c t c g a t a a g g t c t t c a t c a c t c c t c c g a a a a a a c c t c c g g a t

8220 SV40 promoter EM7 promoter g g c t t t t g c a a a a a g c t c c c g g g a g c t t g t a t a t c c a t t t t c g g a t c t g a t c a g c a c g t g c c g a a a a c g t t t t t c g a g g g c c c t c g a a c a t a t a g g t a a a a g c c t a g a c t a g t c g t g c a c

8280 EM7 promoter t t g a c a a t t a a t c a t c g g c a t a g t a t a t c g g c a t a g t a t a a t a c g a c a a g g t g a g g a a c t a a c t g t t a a t t a g t a g c c g t a t c a t a t a g c c g t a t c a t a t t a t g c t g t t c c a c t c c t t g a

MauBI 8340 1 5 10 15 MAKLTSAVPVLTARDVAG BleoR a a a c c a t g g c c a a g t t g a c c a g t g c c g t t c c g g t g c t c a c c g c g c g c g a c g t c g c c g g a g t t t g g t a c c g g t t c a a c t g g t c a c g g c a a g g c c a c g a g t g g c g c g c g c t g c a g c g g c c t c

8400 20 25 30 35 AVEFWTDRLGFSRDFVEDDF BleoR c g g t c g a g t t c t g g a c c g a c c g g c t c g g g t t c t c c c g g g a c t t c g t g g a g g a c g a c t t c g g c c a g c t c a a g a c c t g g c t g g c c g a g c c c a a g a g g g c c c t g a a g c a c c t c c t g c t g a a g c

SgrAI 8460 40 45 50 55 AGVVRDDVTLFISAVQDQVV BleoR c c g g t g t g g t c c g g g a c g a c g t g a c c c t g t t c a t c a g c g c g g t c c a g g a c c a g g t g g t g c g g c c a c a c c a g g c c c t g c t g c a c t g g g a c a a g t a g t c g c g c c a g g t c c t g g t c c a c c a c g

8520 60 65 70 75 PDNTLAWVWVRGLDELYAEW BleoR c g g a c a a c a c c c t g g c c t g g g t g t g g g t g c g c g g c c t g g a c g a g c t g t a c g c c g a g t g g t g c c t g t t g t g g g a c c g g a c c c a c a c c c a c g c g c c g g a c c t g c t c g a c a t g c g g c t c a c c a

FseI 8580 80 85 90 95 SEVVSTNFRDASGPAMTEIG BleoR c g g a g g t c g t g t c c a c g a a c t t c c g g g a c g c c t c c g g g c c g g c c a t g a c c g a g a t c g g c g g c c t c c a g c a c a g g t g c t t g a a g g c c c t g c g g a g g c c c g g c c g g t a c t g g c t c t a g c c g c

8640 100 105 110 115 EQPWGREFALRDPAGNCVHF BleoR a g c a g c c g t g g g g g c g g g a g t t c g c c c t g c g c g a c c c g g c c g g c a a c t g c g t g c a c t t c g t c g t c g g c a c c c c c g c c c t c a a g c g g g a c g c g c t g g g c c g g c c g t t g a c g c a c g t g a a g c

8700 120 125 VAEEQD* BleoR t g g c c g a g g a g c a g g a c t g a c a c g t g c t a c g a g a t t t c g a t t c c a c c g c c g c c t t c t a t g a c c g g c t c c t c g t c c t g a c t g t g c a c g a t g c t c t a a a g c t a a g g t g g c g g c g g a a g a t a c

8760 a a a g g t t g g g c t t c g g a a t c g t t t t c c g g g a c g c c g g c t g g a t g a t c c t c c a g c g c g g g g t t t c c a a c c c g a a g c c t t a g c a a a a g g c c c t g c g g c c g a c c t a c t a g g a g g t c g c g c c c c

8820 SV40 poly(A) signal a t c t c a t g c t g g a g t t c t t c g c c c a c c c c a a c t t g t t t a t t g c a g c t t a t a a t g g t t a c a t a g a g t a c g a c c t c a a g a a g c g g g t g g g g t t g a a c a a a t a a c g t c g a a t a t t a c c a a t g t

8880 SV40 poly(A) signal C G T T A T C G T A G T G T T T A A A G SV40pA-R a a t a a a g c a a t a g c a t c a c a a a t t t c a c a a a t a a a g c a t t t t t t t c a c t g c a t t c t a g t t t t a t t t c g t t a t c g t a g t g t t t a a a g t g t t t a t t t c g t a a a a a a a g t g a c g t a a g a t c a a

BstZ17I 8940 SV40 poly(A) signal G T G G T T T G T C C A A A C T C A T C EBV-rev g t g g t t t g t c c a a a c t c a t c a a t g t a t c t t a t c a t g t c t g t a t a c c g t c g a c c t c t a g c t c a c c a a a c a g g t t t g a g t a g t t a c a t a g a a t a g t a c a g a c a t a t g g c a g c t g g a g a t c g a

9000 M13 rev lac operator C A G T A T C G A C A A A G G A C M13 Reverse G G A C A C A C T T T A A C A A T A G G C G A M13/pUC Reverse a g a g c t t g g c g t a a t c a t g g t c a t a g c t g t t t c c t g t g t g a a a t t g t t a t c c g c t c a c a a t c t c g a a c c g c a t t a g t a c c a g t a t c g a c a a a g g a c a c a c t t t a a c a a t a g g c g a g t g t t

9060 -10 -35 lac promoter CAP binding site t t c c a c a c a a c a t a c g a g c c g g a a g c a t a a a g t g t a a a g c c t g g g g t g c c t a a t g a g t g a a a g g t g t g t t g t a t g c t c g g c c t t c g t a t t t c a c a t t t c g g a c c c c a c g g a t t a c t c a c t

9120 CAP binding site g c t a a c t c a c a t t a a t t g c g t t g c g c t c a c t g c c c g c t t t c c a g t c g g g a a a c c t g t c g t c g a t t g a g t g t a a t t a a c g c a a c g c g a g t g a c g g g c g a a a g g t c a g c c c t t t g g a c a g c a

9180 g c c a g c t g c a t t a a t g a a t c g g c c a a c g c g c g g g g a g a g g c g g t t t g c g t a t t g g g c g c t c g g t c g a c g t a a t t a c t t a g c c g g t t g c g c g c c c c t c t c c g c c a a a c g c a t a a c c c g c g a

9240 G A G C G A G T G A C T G A G C G A L4440 c t t c c g c t t c c t c g c t c a c t g a c t c g c t g c g c t c g g t c g t t c g g c t g c g g c g a g c g g t a t g a a g g c g a a g g a g c g a g t g a c t g a g c g a c g c g a g c c a g c a a g c c g a c g c c g c t c g c c a t a

9300 c a g c t c a c t c a a a g g c g g t a a t a c g g t t a t c c a c a g a a t c a g g g g a t a a c g c a g g a a a g a g t c g a g t g a g t t t c c g c c a t t a t g c c a a t a g g t g t c t t a g t c c c c t a t t g c g t c c t t t c t

PciI 9360 a c a t g t g a g c a a a a g g c c a g c a a a a g g c c a g g a a c c g t a a a a a g g c c g c g t t g c t g g c g t t g t a c a c t c g t t t t c c g g t c g t t t t c c g g t c c t t g g c a t t t t t c c g g c g c a a c g a c c g c a

9420 ori t t t t c c a t a g g c t c c g c c c c c c t g a c g a g c a t c a c a a a a a t c g a c g c t c a a g t c a g a g g t a a a a g g t a t c c g a g g c g g g g g g a c t g c t c g t a g t g t t t t t a g c t g c g a g t t c a g t c t c c a

9480 ori T T T C T A T G G T C C G C A A A G G G pBR322ori-F g g c g a a a c c c g a c a g g a c t a t a a a g a t a c c a g g c g t t t c c c c c t g g a a g c t c c c t c g t g c c c g c t t t g g g c t g t c c t g a t a t t t c t a t g g t c c g c a a a g g g g g a c c t t c g a g g g a g c a c g

9540 ori g c t c t c c t g t t c c g a c c c t g c c g c t t a c c g g a t a c c t g t c c g c c t t t c t c c c t t c g g g a a c g a g a g g a c a a g g c t g g g a c g g c g a a t g g c c t a t g g a c a g g c g g a a a g a g g g a a g c c c t t

9600 ori g c g t g g c g c t t t c t c a t a g c t c a c g c t g t a g g t a t c t c a g t t c g g t g t a g g t c g t t c g c t c g c a c c g c g a a a g a g t a t c g a g t g c g a c a t c c a t a g a g t c a a g c c a c a t c c a g c a a g c g a

9660 ori c c a a g c t g g g c t g t g t g c a c g a a c c c c c c g t t c a g c c c g a c c g c t g c g c c t t a t c c g g t a g g t t c g a c c c g a c a c a c g t g c t t g g g g g g c a a g t c g g g c t g g c g a c g c g g a a t a g g c c a t

9720 ori a c t a t c g t c t t g a g t c c a a c c c g g t a a g a c a c g a c t t a t c g c c a c t g g c a g c a g c c a c t g t g a t a g c a g a a c t c a g g t t g g g c c a t t c t g t g c t g a a t a g c g g t g a c c g t c g t c g g t g a c

9780 ori g t a a c a g g a t t a g c a g a g c g a g g t a t g t a g g c g g t g c t a c a g a g t t c t t g a a g t g g t g g c c a t t g t c c t a a t c g t c t c g c t c c a t a c a t c c g c c a c g a t g t c t c a a g a a c t t c a c c a c c g

9840 ori c t a a c t a c g g c t a c a c t a g a a g a a c a g t a t t t g g t a t c t g c g c t c t g c t g a a g c c a g t t a g a t t g a t g c c g a t g t g a t c t t c t t g t c a t a a a c c a t a g a c g c g a g a c g a c t t c g g t c a a t

9900 ori c c t t c g g a a a a a g a g t t g g t a g c t c t t g a t c c g g c a a a c a a a c c a c c g c t g g t a g c g g t g g g a a g c c t t t t t c t c a a c c a t c g a g a a c t a g g c c g t t t g t t t g g t g g c g a c c a t c g c c a c

9960 ori g t t t t t t t g t t t g c a a g c a g c a g a t t a c g c g c a g a a a a a a a g g a t c t c a a g a a g a t c c t t c a a a a a a a c a a a c g t t c g t c g t c t a a t g c g c g t c t t t t t t t c c t a g a g t t c t t c t a g g a a

10,020 t g a t c t t t t c t a c g g g g t c t g a c g c t c a g t g g a a c g a a a a c t c a c g t t a a g g g a t t t t g g a c t a g a a a a g a t g c c c c a g a c t g c g a g t c a c c t t g c t t t t g a g t g c a a t t c c c t a a a a c c

10,080 t c a t g a g a t t a t c a a a a a g g a t c t t c a c c t a g a t c c t t t t a a a t t a a a a a t g a a g t t t t a a g t a c t c t a a t a g t t t t t c c t a g a a g t g g a t c t a g g a a a a t t t a a t t t t t a c t t c a a a a t

10,140 285 *WHKILS AmpR *WHKILS a a t c a a t c t a a a g t a t a t a t g a g t a a a c t t g g t c t g a c a g t t a c c a a t g c t t a a t c a g t g t t a g t t a g a t t t c a t a t a t a c t c a t t t g a a c c a g a c t g t c a a t g g t t a c g a a t t a g t c a c

AhdI 10,200 280 275 270 265 AGIEAIQRNREDMTAQSGTT AmpR AGIEAIQRNREDMTAQSGTT a g g c a c c t a t c t c a g c g a t c t g t c t a t t t c g t t c a t c c a t a g t t g c c t g a c t c c c c g t c g t c c g t g g a t a g a g t c g c t a g a c a g a t a a a g c a a g t a g g t a t c a a c g g a c t g a g g g g c a g c

10,260 260 255 250 245 YIVVIRSPKGDPGLAAIIGR AmpR YIVVIRSPKGDPGLAAIIGR t g t a g a t a a c t a c g a t a c g g g a g g g c t t a c c a t c t g g c c c c a g t g c t g c a a t g a t a c c g c a c a t c t a t t g a t g c t a t g c c c t c c c g a a t g g t a g a c c g g g g t c a c g a c g t t a c t a t g g c g

10,320 240 235 230 225 SGREGAGSKDAIFWGAPLAS AmpR SGREGAGSKDAIFWGAPLAS g a g a c c c a c g c t c a c c g g c t c c a g a t t t a t c a g c a a t a a a c c a g c c a g c c g g a a g g g c c g c t c t g g g t g c g a g t g g c c g a g g t c t a a a t a g t c g t t a t t t g g t c g g t c g g c c t t c c c g g c

10,380 220 215 210 205 RLLPGAVKDAEMWDILQQRS AmpR RLLPGAVKDAEMWDILQQRS a g c g c a g a a g t g g t c c t g c a a c t t t a t c c g c c t c c a t c c a g t c t a t t a a t t g t t g c c g g g t c g c g t c t t c a c c a g g a c g t t g a a a t a g g c g g a g g t a g g t c a g a t a a t t a a c a a c g g c c c

FspI 10,440 200 195 190 185 ALTLLEGTLLKRLTTAMAVP AmpR ALTLLEGTLLKRLTTAMAVP a a g c t a g a g t a a g t a g t t c g c c a g t t a a t a g t t t g c g c a a c g t t g t t g c c a t t g c t a c a g t t c g a t c t c a t t c a t c a a g c g g t c a a t t a t c a a a c g c g t t g c a a c a a c g g t a a c g a t g t c

10,500 180 175 170 165 MTTDREDNPIAENLEPEWRD AmpR MTTDREDNPIAENLEPEWRD g c a t c g t g g t g t c a c g c t c g t c g t t t g g t a t g g c t t c a t t c a g c t c c g g t t c c c a a c g a t c g t a g c a c c a c a g t g c g a g c a g c a a a c c a t a c c g a a g t a a g t c g a g g c c a a g g g t t g c t a

10,560 160 155 150 145 LRTVHDGMNHLFATLEKPGG AmpR LRTVHDGMNHLFATLEKPGG c a a g g c g a g t t a c a t g a t c c c c c a t g t t g t g c a a a a a a g c g g t t a g c t c c t t c g g t c c t c g t t c c g c t c a a t g t a c t a g g g g g t a c a a c a c g t t t t t t c g c c a a t c g a g g a a g c c a g g a g

PvuI 10,620 140 135 130 125 ITTLLLNAATNDSMTIAASC AmpR ITTLLLNAATNDSMTIAASC c g a t c g t t g t c a g a a g t a a g t t g g c c g c a g t g t t a t c a c t c a t g g t t a t g g c a g c a c t g c g c t a g c a a c a g t c t t c a t t c a a c c g g c g t c a c a a t a g t g a g t a c c a a t a c c g t c g t g a c g

10,680 120 115 110 105 LERVTMGDTLHKETVPSYEV AmpR LERVTMGDTLHKETVPSYEV a t a a t t c t c t t a c t g t c a t g c c a t c c g t a a g a t g c t t t t c t g t g a c t g g t g a g t a c t c a a t a t t a a g a g a a t g a c a g t a c g g t a g g c a t t c t a c g a a a a g a c a c t g a c c a c t c a t g a g t t

10,740 100 95 90 85 LDNQSYHIRRGLQEQGADIR AmpR LDNQSYHIRRGLQEQGADIR c c a a g t c a t t c t g a g a a t a g t g t a t g c g g c g a c c g a g t t g c t c t t g c c c g g c g t c a a t a c g g t t c a g t a a g a c t c t t a t c a c a t a c g c c g c t g g c t c a a c g a g a a c g g g c c g c a g t t a t g

10,800 80 75 70 65 SLVAGCLLVKFTSMMPFREE AmpR A T A A T A C C G C G C C A C A T A G C Amp-R SLVAGCLLVKFTSMMPFREE g g g a t a a t a c c g c g c c a c a t a g c a g a a c t t t a a a a g t g c t c a t c a t t g g a a a a c g t t c t t c c c t a t t a t g g c g c g g t g t a t c g t c t t g a a a t t t t c a c g a g t a g t a a c c t t t t g c a a g a a

10,860 60 55 50 45 PRFSELIKGSNLDLEIYGVR AmpR PRFSELIKGSNLDLEIYGVR c g g g g c g a a a a c t c t c a a g g a t c t t a c c g c t g t t g a g a t c c a g t t c g a t g t a a c c c a c t c g c c c c g c t t t t g a g a g t t c c t a g a a t g g c g a c a a c t c t a g g t c a a g c t a c a t t g g g t g a g

10,920 40 35 30 25 AGLQDEADKVKVLTEPH signal sequence AFV AmpR AGLQDEADKVKVLTEPHAFV g t g c a c c c a a c t g a t c t t c a g c a t c t t t t a c t t t c a c c a g c g t t t c t g g g t g a g c a a a a a c a c g t g g g t t g a c t a g a a g t c g t a g a a a a t g a a a g t g g t c g c a a a g a c c c a c t c g t t t t t

10,980 20 15 10 5 1 signal sequence PLCFAAFFPILAVRFHQISM AmpR PLCFAAFFPILAVRFHQISM c a g g a a g g c a a a a t g c c g c a a a a a a g g g a a t a a g g g c g a c a c g g a a a t g t t g a a t a c t c a g t c c t t c c g t t t t a c g g c g t t t t t t c c c t t a t t c c c g c t g t g c c t t t a c a a c t t a t g a g t

SspI 11,040 AmpR AmpR promoter t a c t c t t c c t t t t t c a a t a t t a t t g a a g c a t t t a t c a g g g t t a t t g t c t c a t g a g c g g a t a t g a g a a g g a a a a a g t t a t a a t a a c t t c g t a a a t a g t c c c a a t a a c a g a g t a c t c g c c t a

11,100 AmpR promoter a c a t a t t t g a a t g t a t t t a g a a a a a t a a a c a a a t a g g g g t t c c g c g c a c a t t t c c c c g a a t g t a t a a a c t t a c a t a a a t c t t t t t a t t t g t t t a t c c c c a a g g c g c g t g t a a a g g g g c t t

SgrDI 11,160 a a g t g c c a c c t g a c g t c g a c g g a t c g g g a g a t c t c c c g a t c c c c t a t g g t g c a c t c t c a g t t c a c g g t g g a c t g c a g c t g c c t a g c c c t c t a g a g g g c t a g g g g a t a c c a c g t g a g a g t c

11,220 A T G T T A G A C G A G A C T A C G G C pRS-marker t a c a a t c t g c t c t g a t g c c g c a t a g t t a a g c c a g t a t c t g c t c c c t g c t t g t g t g t t g g a a t g t t a g a c g a g a c t a c g g c g t a t c a a t t c g g t c a t a g a c g a g g g a c g a a c a c a c a a c c t

3ʹ 5ʹ 11,264 g g t c g c t g a g t a g t g c g c g a g c a a a a t t t a a g c t a c a a c a a g g c c c a g c g a c t c a t c a c g c g c t c g t t t t a a a t t c g a t g t t g t t c c g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.