Addgene pIRIGF-IKZF3 Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pIRIGF-IKZF3 Sequencing Result


Map View

SbfI (9045) LNCX (8923 .. 8947) CMV-F (8877 .. 8897) SnaBI (8698) NdeI (8592) SpeI (8357) MluI (8336) NruI (8316) Bpu10I (8288) pRS-marker (8152 .. 8171) BglII (8120) pBR322ori-F (7953 .. 7972) BsaI (7154) NmeAIII (7073) FspI (6997) PvuI (6851) Amp-R (6651 .. 6670) SspI (6415) BamHI (6297) BspDI - ClaI (6290) HpaI (6195) SV40pA-R (6139 .. 6158) EBV-rev (6085 .. 6104) NotI (6052) XbaI (6045) NaeI (5907) NgoMIV (5905) F1ori-R (5867 .. 5886) PvuII (5756) SgrAI (5283) PpuMI (5034) AgeI (0) PaeR7I - PspXI - XhoI (1539) PmeI (1548) BstEII (2730) PasI (2952) TspMI - XmaI (3295) SmaI (3297) StuI (3382) PflFI - Tth111I (3567) EcoRV (3714) LucNrev (3921 .. 3939) pIRIGF-IKZF3 9086 bp

Sequence View

AgeI 5ʹ 3ʹ 60 IRES2 c c g g t a t g t a a t c c g c c c c t c t c c c t c c c c c c c c c c t a a c g t t a c t g g c c g a a g c c g c t t g g c c a t a c a t t a g g c g g g g a g a g g g a g g g g g g g g g g a t t g c a a t g a c c g g c t t c g g c g a a

120 IRES2 g g a a t a a g g c c g g t g t g c g t t t g t c t a t a t g t t a t t t t c c a c c a t a t t g c c g t c t t t t g g c c t t a t t c c g g c c a c a c g c a a a c a g a t a t a c a a t a a a a g g t g g t a t a a c g g c a g a a a a c c

180 IRES2 c a a t g t g a g g g c c c g g a a a c c t g g c c c t g t c t t c t t g a c g a g c a t t c c t a g g g g t c t t t c g t t a c a c t c c c g g g c c t t t g g a c c g g g a c a g a a g a a c t g c t c g t a a g g a t c c c c a g a a a g

240 IRES2 c c c t c t c g c c a a a g g a a t g c a a g g t c t g t t g a a t g t c g t g a a g g a a g c a g t t c c t c t g g a g g g a g a g c g g t t t c c t t a c g t t c c a g a c a a c t t a c a g c a c t t c c t t c g t c a a g g a g a c c t

300 IRES2 a g c t t c t t g a a g a c a a a c a a c g t c t g t a g c g a c c c t t t g c a g g c a g c g g a a c c c c c c a c c t c g a a g a a c t t c t g t t t g t t g c a g a c a t c g c t g g g a a a c g t c c g t c g c c t t g g g g g g t g g

360 IRES2 t g g c g a c a g g t g c c t c t g c g g c c a a a a g c c a c g t g t a t a a g a t a c a c c t g c a a a g g c g g c a c c g c t g t c c a c g g a g a c g c c g g t t t t c g g t g c a c a t a t t c t a t g t g g a c g t t t c c g c c g

420 IRES2 a c a a c c c c a g t g c c a c g t t g t g a g t t g g a t a g t t g t g g a a a g a g t c a a a t g g c t c t c c t c t g t t g g g g t c a c g g t g c a a c a c t c a a c c t a t c a a c a c c t t t c t c a g t t t a c c g a g a g g a g

480 IRES2 a a g c g t a t t c a a c a a g g g g c t g a a g g a t g c c c a g a a g g t a c c c c a t t g t a t g g g a t c t g a t t c g c a t a a g t t g t t c c c c g a c t t c c t a c g g g t c t t c c a t g g g g t a a c a t a c c c t a g a c t

540 IRES2 t c t g g g g c c t c g g t a c a c a t g c t t t a c a t g t g t t t a g t c g a g g t t a a a a a a a c g t c t a g g a g a c c c c g g a g c c a t g t g t a c g a a a t g t a c a c a a a t c a g c t c c a a t t t t t t t g c a g a t c c

600 ATG IRES2 MAT c c c c c c g a a c c a c g g g g a c g t g g t t t t c c t t t g a a a a a c a c g a t g a t a a t a t g g c c a c a a g g g g g g c t t g g t g c c c c t g c a c c a a a a g g a a a c t t t t t g t g c t a c t a t t a t a c c g g t g t t

660 1 5 10 15 MTSKVYDPEQRKRMITGPQ IRES2 Rluc TMTSKVYDPEQRKRMITGPQ c c a t g a c t t c g a a a g t t t a t g a t c c a g a a c a a a g g a a a c g g a t g a t a a c t g g t c c g c a g t g g t a c t g a a g c t t t c a a a t a c t a g g t c t t g t t t c c t t t g c c t a c t a t t g a c c a g g c g t c a

720 20 25 30 35 WWARCKQMNVLDSFINYYDS Rluc WWARCKQMNVLDSFINYYDS g g t g g g c c a g a t g t a a a c a a a t g a a t g t t c t t g a t t c a t t t a t t a a t t a t t a t g a t t c a g c c a c c c g g t c t a c a t t t g t t t a c t t a c a a g a a c t a a g t a a a t a a t t a a t a a t a c t a a g t c

780 40 45 50 55 EKHAENAVIFLHGNAASSYL Rluc EKHAENAVIFLHGNAASSYL a a a a a c a t g c a g a a a a t g c t g t t a t t t t t t t a c a t g g t a a c g c g g c c t c t t c t t a t t t a t t t t t t g t a c g t c t t t t a c g a c a a t a a a a a a a t g t a c c a t t g c g c c g g a g a a g a a t a a a t a

840 60 65 70 75 WRHVVPHIEPVARCIIPDLI Rluc WRHVVPHIEPVARCIIPDLI g g c g a c a t g t t g t g c c a c a t a t t g a g c c a g t a g c g c g g t g t a t t a t a c c a g a c c t t a t t g c c g c t g t a c a a c a c g g t g t a t a a c t c g g t c a t c g c g c c a c a t a a t a t g g t c t g g a a t a a c

900 80 85 90 95 GMGKSGKSGNGSYRLLDHYK Rluc GMGKSGKSGNGSYRLLDHYK g t a t g g g c a a a t c a g g c a a a t c t g g t a a t g g t t c t t a t a g g t t a c t t g a t c a t t a c a a a t c a t a c c c g t t t a g t c c g t t t a g a c c a t t a c c a a g a a t a t c c a a t g a a c t a g t a a t g t t t a

960 100 105 110 115 YLTAWFELLNLPKKIIFVGH Rluc YLTAWFELLNLPKKIIFVGH a t c t t a c t g c a t g g t t t g a a c t t c t t a a t t t a c c a a a g a a g a t c a t t t t t g t c g g c c a t g t a g a a t g a c g t a c c a a a c t t g a a g a a t t a a a t g g t t t c t t c t a g t a a a a a c a g c c g g t a c

1020 120 125 130 135 DWGACLAFHYSYEHQDKIKA Rluc DWGACLAFHYSYEHQDKIKA a t t g g g g t g c t t g t t t g g c a t t t c a t t a t a g c t a t g a g c a t c a a g a t a a g a t c a a a g c a a t a a c c c c a c g a a c a a a c c g t a a a g t a a t a t c g a t a c t c g t a g t t c t a t t c t a g t t t c g t t

1080 140 145 150 155 IVHAESVVDVIESWDEWPDI Rluc IVHAESVVDVIESWDEWPDI t a g t t c a c g c t g a a a g t g t a g t a g a t g t g a t t g a a t c a t g g g a t g a a t g g c c t g a t a t t g a t c a a g t g c g a c t t t c a c a t c a t c t a c a c t a a c t t a g t a c c c t a c t t a c c g g a c t a t a a c

1140 160 165 170 175 EEDIALIKSEEGEKMVLENN Rluc EEDIALIKSEEGEKMVLENN a a g a a g a t a t t g c g t t g a t c a a a t c t g a a g a a g g a g a a a a a a t g g t t t t g g a g a a t a a c t t t c t t c t a t a a c g c a a c t a g t t t a g a c t t c t t c c t c t t t t t t a c c a a a a c c t c t t a t t g a

1200 180 185 190 195 FFVETMLPSKIMRKLEPEEF Rluc FFVETMLPSKIMRKLEPEEF t c t t c g t g g a a a c c a t g t t g c c a t c a a a a a t c a t g a g a a a g t t a g a a c c a g a a g a a t t t g a g a a g c a c c t t t g g t a c a a c g g t a g t t t t t a g t a c t c t t t c a a t c t t g g t c t t c t t a a a c

1260 200 205 210 215 AAYLEPFKEKGEVRRPTLSW Rluc AAYLEPFKEKGEVRRPTLSW c a g c a t a t c t t g a a c c a t t c a a a g a g a a a g g t g a a g t t c g t c g t c c a a c a t t a t c a t g g c g t c g t a t a g a a c t t g g t a a g t t t c t c t t t c c a c t t c a a g c a g c a g g t t g t a a t a g t a c c g

1320 220 225 230 235 PREIPLVKGGKPDVVQIVRN Rluc PREIPLVKGGKPDVVQIVRN c t c g t g a a a t c c c g t t a g t a a a a g g t g g t a a a c c t g a c g t t g t a c a a a t t g t t a g g a a t t g a g c a c t t t a g g g c a a t c a t t t t c c a c c a t t t g g a c t g c a a c a t g t t t a a c a a t c c t t a a

1380 240 245 250 255 YNAYLRASDDLPKMFIESDP Rluc YNAYLRASDDLPKMFIESDP a t a a t g c t t a t c t a c g t g c a a g t g a t g a t t t a c c a a a a a t g t t t a t t g a a t c g g a c c c a g t a t t a c g a a t a g a t g c a c g t t c a c t a c t a a a t g g t t t t t a c a a a t a a c t t a g c c t g g g t c

1440 260 265 270 275 GFFSNAIVEGAKKFPNTEFV Rluc GFFSNAIVEGAKKFPNTEFV g a t t c t t t t c c a a t g c t a t t g t t g a a g g t g c c a a g a a g t t t c c t a a t a c t g a a t t t g t c a c t a a g a a a a g g t t a c g a t a a c a a c t t c c a c g g t t c t t c a a a g g a t t a t g a c t t a a a c a g t

1500 280 285 290 295 KVKGLHFSQEDAPDEMGKYI Rluc KVKGLHFSQEDAPDEMGKYI a a g t a a a a g g t c t t c a t t t t t c g c a a g a a g a t g c a c c t g a t g a a a t g g g a a a a t a t a t c a t t c a t t t t c c a g a a g t a a a a a g c g t t c t t c t a c g t g g a c t a c t t t a c c c t t t t a t a t a g t

PmeIPaeR7IPspXIXhoI 1560 300 305 310 KSFVERVLKNEQ* Rluc KSFVERVLKNEQ* a a t c g t t c g t t g a g c g a g t t c t c a a a a a t g a a c a a t g a c t c g a g g t t t a a a c t a t a g a g t t t a g c a a g c a a c t c g c t c a a g a g t t t t t a c t t g t t a c t g a g c t c c a a a t t t g a t a t c t c a

1620 a c g a g c c a t g a t a a c t g c a g g t c g a g c a t g c a t c t a g g g c g g c c a a t t c c g c c c c t c t c c t g c t c g g t a c t a t t g a c g t c c a g c t c g t a c g t a g a t c c c g c c g g t t a a g g c g g g g a g a g g

1680 IRES2 c c c c c c c c c t c t c c c t c c c c c c c c c c t a a c g t t a c t g g c c g a a g c c g c t t g g a a t a a g g c g g g g g g g g g a g a g g g a g g g g g g g g g g a t t g c a a t g a c c g g c t t c g g c g a a c c t t a t t c c g

1740 IRES2 c g g t g t g c g t t t g t c t a t a t g t t a t t t t c c a c c a t a t t g c c g t c t t t t g g c a a t g t g a g g g c c a c a c g c a a a c a g a t a t a c a a t a a a a g g t g g t a t a a c g g c a g a a a a c c g t t a c a c t c c

1800 IRES2 g c c c g g a a a c c t g g c c c t g t c t t c t t g a c g a g c a t t c c t a g g g g t c t t t c c c c t c t c g c c c g g g c c t t t g g a c c g g g a c a g a a g a a c t g c t c g t a a g g a t c c c c a g a a a g g g g a g a g c g g

1860 IRES2 a a a g g a a t g c a a g g t c t g t t g a a t g t c g t g a a g g a a g c a g t t c c t c t g g a a g c t t c t t g a t t t c c t t a c g t t c c a g a c a a c t t a c a g c a c t t c c t t c g t c a a g g a g a c c t t c g a a g a a c t

1920 IRES2 a g a c a a a c a a c g t c t g t a g c g a c c c t t t g c a g g c a g c g g a a c c c c c c a c c t g g c g a c a g g t c t g t t t g t t g c a g a c a t c g c t g g g a a a c g t c c g t c g c c t t g g g g g g t g g a c c g c t g t c c

1980 IRES2 t g c c t c t g c g g c c a a a a g c c a c g t g t a t a a g a t a c a c c t g c a a a g g c g g c a c a a c c c c a g a c g g a g a c g c c g g t t t t c g g t g c a c a t a t t c t a t g t g g a c g t t t c c g c c g t g t t g g g g t c

2040 IRES2 t g c c a c g t t g t g a g t t g g a t a g t t g t g g a a a g a g t c a a a t g g c t c t c c t c a a g c g t a t t c a c g g t g c a a c a c t c a a c c t a t c a a c a c c t t t c t c a g t t t a c c g a g a g g a g t t c g c a t a a g

2100 IRES2 a a c a a g g g g c t g a a g g a t g c c c a g a a g g t a c c c c a t t g t a t g g g a t c t g a t c t g g g g c c t t t g t t c c c c g a c t t c c t a c g g g t c t t c c a t g g g g t a a c a t a c c c t a g a c t a g a c c c c g g a

2160 IRES2 MLYMCLVEVKKTSRPPE c g g t g c a c a t g c t t t a c a t g t g t t t a g t c g a g g t t a a a a a a a c g t c t a g g c c c c c c g a a c g c c a c g t g t a c g a a a t g t a c a c a a a t c a g c t c c a a t t t t t t t g c a g a t c c g g g g g g c t t g

2220 ATG IRES2 PRGRGFPLKNTMISLPQPTR c a c g g g g a c g t g g t t t t c c t t t g a a a a a c a c g a t g a t a a g c t t g c c a c a a c c c a c a a g g a g t g c c c c t g c a c c a a a a g g a a a c t t t t t g t g c t a c t a t t c g a a c g g t g t t g g g t g t t c c t

2280 attB1 RRPSTSLYKKVGMEDIQTNA g a c g a c c t t c c a c a a g t t t g t a c a a a a a a g t t g g c a t g g a a g a t a t a c a a a c a a a t g c g g c t g c t g g a a g g t g t t c a a a c a t g t t t t t t c a a c c g t a c c t t c t a t a t g t t t g t t t a c g c c

2340 ELKSTQEQSVPAESAAVLND a a c t g a a a a g c a c t c a g g a g c a g t c t g t g c c c g c a g a a a g t g c a g c g g t t t t g a a t g a c t t t g a c t t t t c g t g a g t c c t c g t c a g a c a c g g g c g t c t t t c a c g t c g c c a a a a c t t a c t g a

2400 YSLTKSHEMENVDSGEGPAN a c a g t t t a a c c a a a t c t c a t g a a a t g g a a a a t g t g g a c a g t g g a g a a g g c c c a g c c a a t g t g t c a a a t t g g t t t a g a g t a c t t t a c c t t t t a c a c c t g t c a c c t c t t c c g g g t c g g t t a c

2460 EDEDIGDDSMKVKDEYSERD a a g a t g a a g a c a t a g g a g a t g a t t c a a t g a a a g t g a a a g a t g a a t a c a g t g a a a g a g a t g t t c t a c t t c t g t a t c c t c t a c t a a g t t a c t t t c a c t t t c t a c t t a t g t c a c t t t c t c t a c

2520 ENVLKSEPMGNAEEPEIPYS a g a a t g t t t t a a a g t c a g a a c c c a t g g g a a a t g c a g a a g a g c c t g a a a t c c c t t a c a g c t t c t t a c a a a a t t t c a g t c t t g g g t a c c c t t t a c g t c t t c t c g g a c t t t a g g g a a t g t c g a

2580 YSREYNEYENIKLERHVVSF a t t c a a g a g a a t a t a a t g a a t a t g a a a a c a t t a a g t t g g a g a g a c a t g t t g t c t c a t t c g t a a g t t c t c t t a t a t t a c t t a t a c t t t t g t a a t t c a a c c t c t c t g t a c a a c a g a g t a a g c

2640 DSSRPTSGKMNCDVCGLSCI a t a g t a g c a g g c c a a c c a g t g g a a a g a t g a a c t g c g a t g t g t g t g g a t t a t c c t g c a t c a t a t c a t c g t c c g g t t g g t c a c c t t t c t a c t t g a c g c t a c a c a c a c c t a a t a g g a c g t a g t

2700 SFNVLMVHKRSHTGERPFQC g c t t c a a t g t c t t a a t g g t t c a t a a g c g a a g c c a t a c t g g t g a a c g c c c a t t c c a g t g t a c g a a g t t a c a g a a t t a c c a a g t a t t c g c t t c g g t a t g a c c a c t t g c g g g t a a g g t c a c a t

BstEII 2760 NQCGASFTQKGNLLRHIKLH a t c a g t g t g g g g c a t c t t t t a c t c a g a a a g g t a a c c t c c t c c g c c a c a t t a a a c t g c a c a t a g t c a c a c c c c g t a g a a a a t g a g t c t t t c c a t t g g a g g a g g c g g t g t a a t t t g a c g t g t

2820 TGEKPFKCHLCNYACQRRDA c a g g g g a a a a a c c t t t t a a g t g t c a c c t c t g c a a c t a t g c a t g c c a a a g a a g a g a t g c g c g t c c c c t t t t t g g a a a a t t c a c a g t g g a g a c g t t g a t a c g t a c g g t t t c t t c t c t a c g c g

2880 LTGHLRTHSVEKPYKCEFCG t c a c g g g g c a t c t t a g g a c a c a t t c t g t g g a g a a a c c c t a c a a a t g t g a g t t t t g t g g a a a g t g c c c c g t a g a a t c c t g t g t a a g a c a c c t c t t t g g g a t g t t t a c a c t c a a a a c a c c t t

2940 RSYKQRSSLEEHKERCRTFL g g a g t t a c a a g c a g a g a a g t t c c c t t g a g g a g c a c a a g g a g c g c t g c c g t a c a t t t c t t c c c t c a a t g t t c g t c t c t t c a a g g g a a c t c c t c g t g t t c c t c g c g a c g g c a t g t a a a g a a g

PasI 3000 QSTDPGDTASAEARHIKAEM a g a g c a c t g a c c c a g g g g a c a c t g c a a g t g c g g a g g c a a g a c a c a t c a a a g c a g a g a t g g t c t c g t g a c t g g g t c c c c t g t g a c g t t c a c g c c t c c g t t c t g t g t a g t t t c g t c t c t a c c

3060 GSERALVLDRLASNVAKRKS g a a g t g a a a g a g c t c t c g t a c t g g a c a g a t t a g c a a g c a a t g t g g c a a a a c g a a a a a g c t c t t c a c t t t c t c g a g a g c a t g a c c t g t c t a a t c g t t c g t t a c a c c g t t t t g c t t t t t c g a

3120 SMPQKFIGEKRHCFDVNYNS c a a t g c c t c a g a a a t t c a t t g g t g a g a a g c g c c a c t g c t t t g a t g t c a a c t a t a a t t c a a g t t a c g g a g t c t t t a a g t a a c c a c t c t t c g c g g t g a c g a a a c t a c a g t t g a t a t t a a g t t

3180 SYMYEKESELIQTRMMDQAI g t t a c a t g t a t g a g a a a g a g a g t g a g c t c a t a c a g a c c c g c a t g a t g g a c c a a g c c a t c a c a a t g t a c a t a c t c t t t c t c t c a c t c g a g t a t g t c t g g g c g t a c t a c c t g g t t c g g t a g t

3240 NNAISYLGAEALRPLVQTPP a t a a c g c c a t c a g c t a t c t t g g c g c c g a a g c c c t g c g c c c c t t g g t c c a g a c a c c g c c t g t a t t g c g g t a g t c g a t a g a a c c g c g g c t t c g g g a c g c g g g g a a c c a g g t c t g t g g c g g a c

TspMIXmaISmaI 3300 APTSEMVPVISSMYPIALTR c t c c c a c c t c g g a g a t g g t t c c a g t t a t c a g c a g c a t g t a t c c c a t a g c c c t c a c c c g g g g a g g g t g g a g c c t c t a c c a a g g t c a a t a g t c g t c g t a c a t a g g g t a t c g g g a g t g g g c c c

3360 AEMSNGAPQELEKKSIHLPE c t g a g a t g t c a a a c g g t g c c c c t c a a g a g c t g g a a a a g a a a a g c a t c c a c c t t c c a g a g a g a c t c t a c a g t t t g c c a c g g g g a g t t c t c g a c c t t t t c t t t t c g t a g g t g g a a g g t c t c t

StuI 3420 KSVPSERGLSPNNSGHDSTD a g a g c g t g c c t t c t g a g a g a g g c c t c t c t c c c a a c a a t a g t g g c c a c g a c t c c a c g g a c a t c t c g c a c g g a a g a c t c t c t c c g g a g a g a g g g t t g t t a t c a c c g g t g c t g a g g t g c c t g t

3480 TDSNHEERQNHIYQQNHMVL c t g a c a g c a a c c a t g a a g a a c g c c a g a a t c a c a t c t a t c a g c a a a a t c a c a t g g t c c t g t g a c t g t c g t t g g t a c t t c t t g c g g t c t t a g t g t a g a t a g t c g t t t t a g t g t a c c a g g a c a

3540 SRARNGMPLLKEVPRSYELL c t c g g g c c c g c a a t g g g a t g c c a c t t c t g a a g g a g g t t c c c c g c t c t t a c g a a c t c c t c a g a g c c c g g g c g t t a c c c t a c g g t g a a g a c t t c c t c c a a g g g g c g a g a a t g c t t g a g g a g t

Tth111IPflFI 3600 KPPPICPRDSVKVINKEGEV a g c c c c c g c c c a t c t g c c c a a g a g a c t c c g t c a a a g t g a t c a a c a a g g a a g g g g a g g t g a t c g g g g g c g g g t a g a c g g g t t c t c t g a g g c a g t t t c a c t a g t t g t t c c t t c c c c t c c a c t

3660 MDVYRCDHCRVLFLDYVMFT t g g a t g t g t a t c g g t g t g a c c a c t g c c g c g t c c t c t t c c t g g a c t a t g t g a t g t t c a c g a a c c t a c a c a t a g c c a c a c t g g t g a c g g c g c a g g a g a a g g a c c t g a t a c a c t a c a a g t g c t

EcoRV 3720 IHMGCHGFRDPFECNMCGYR t t c a c a t g g g c t g c c a c g g c t t c c g t g a c c c t t t c g a g t g t a a c a t g t g t g g a t a t c g a a a a g t g t a c c c g a c g g t g c c g a a g g c a c t g g g a a a g c t c a c a t t g t a c a c a c c t a t a g c t t

3780 SHDRYEFSSHIARGEHRALL g c c a t g a t c g g t a t g a g t t c t c g t c t c a c a t a g c c a g a g g a g a a c a c a g a g c c c t g c t g a c g g t a c t a g c c a t a c t c a a g a g c a g a g t g t a t c g g t c t c c t c t t g t g t c t c g g g a c g a c t

3840 attB2 KCPTFLYKVVGGGGSGGGGS a g t g c c c a a c t t t c t t g t a c a a a g t g g t g g g t g g a g g c g g t t c a g g t g g c g g a g g a a g c g t c a c g g g t t g a a a g a a c a t g t t t c a c c a c c c a c c t c c g c c a a g t c c a c c g c c t c c t t c g c

3900 1 5 10 15 MEDAKNIKKGPAPFY luciferase GGGGSMEDAKNIKKGPAPFY g a g g c g g t g g a a g t a t g g a a g a c g c c a a a a a c a t a a a g a a a g g c c c g g c g c c a t t c t a t c c t c c g c c a c c t t c a t a c c t t c t g c g g t t t t t g t a t t t c t t t c c g g g c c g c g g t a a g a t a g

3960 20 25 30 35 PLEDGTAGEQLHKAMKRYAL luciferase C C T C T C G T T G A C G T A T T C C LucNrev PLEDGTAGEQLHKAMKRYAL c g c t g g a a g a t g g a a c c g c t g g a g a g c a a c t g c a t a a g g c t a t g a a g a g a t a c g c c c t g g g c g a c c t t c t a c c t t g g c g a c c t c t c g t t g a c g t a t t c c g a t a c t t c t c t a t g c g g g a c c

4020 40 45 50 55 VPGTIAFTDAHIEVDITYAE luciferase VPGTIAFTDAHIEVDITYAE t t c c t g g a a c a a t t g c t t t t a c a g a t g c a c a t a t c g a g g t g g a c a t c a c t t a c g c t g a g t a a g g a c c t t g t t a a c g a a a a t g t c t a c g t g t a t a g c t c c a c c t g t a g t g a a t g c g a c t c a

4080 60 65 70 75 YFEMSVRLAEAMKRYGLNTN luciferase YFEMSVRLAEAMKRYGLNTN a c t t c g a a a t g t c c g t t c g g t t g g c a g a a g c t a t g a a a c g a t a t g g g c t g a a t a c a a a t c t g a a g c t t t a c a g g c a a g c c a a c c g t c t t c g a t a c t t t g c t a t a c c c g a c t t a t g t t t a g

4140 80 85 90 95 HRIVVCSENSLQFFMPVLGA luciferase HRIVVCSENSLQFFMPVLGA a c a g a a t c g t c g t a t g c a g t g a a a a c t c t c t t c a a t t c t t t a t g c c g g t g t t g g g c g c g t t g t c t t a g c a g c a t a c g t c a c t t t t g a g a g a a g t t a a g a a a t a c g g c c a c a a c c c g c g c a

4200 100 105 110 115 LFIGVAVAPANDIYNERELL luciferase LFIGVAVAPANDIYNERELL t a t t t a t c g g a g t t g c a g t t g c g c c c g c g a a c g a c a t t t a t a a t g a a c g t g a a t t g c t c a a t a a a t a g c c t c a a c g t c a a c g c g g g c g c t t g c t g t a a a t a t t a c t t g c a c t t a a c g a g t

4260 120 125 130 135 NSMGISQPTVVFVSKKGLQK luciferase NSMGISQPTVVFVSKKGLQK a c a g t a t g g g c a t t t c g c a g c c t a c c g t g g t g t t c g t t t c c a a a a a g g g g t t g c a a a a a a t g t c a t a c c c g t a a a g c g t c g g a t g g c a c c a c a a g c a a a g g t t t t t c c c c a a c g t t t t t t

4320 140 145 150 155 ILNVQKKLPIIQKIIIMDSK luciferase ILNVQKKLPIIQKIIIMDSK t t t t g a a c g t g c a a a a a a a g c t c c c a a t c a t c c a a a a a a t t a t t a t c a t g g a t t c t a a a a a a a a c t t g c a c g t t t t t t t c g a g g g t t a g t a g g t t t t t t a a t a a t a g t a c c t a a g a t t t t

4380 160 165 170 175 TDYQGFQSMYTFVTSHLPPG luciferase TDYQGFQSMYTFVTSHLPPG c g g a t t a c c a g g g a t t t c a g t c g a t g t a c a c g t t c g t c a c a t c t c a t c t a c c t c c c g g t t g c c t a a t g g t c c c t a a a g t c a g c t a c a t g t g c a a g c a g t g t a g a g t a g a t g g a g g g c c a a

4440 180 185 190 195 FNEYDFVPESFDRDKTIALI luciferase FNEYDFVPESFDRDKTIALI t t a a t g a a t a c g a t t t t g t g c c a g a g t c c t t c g a t a g g g a c a a g a c a a t t g c a c t g a t c a a a t t a c t t a t g c t a a a a c a c g g t c t c a g g a a g c t a t c c c t g t t c t g t t a a c g t g a c t a g t

4500 200 205 210 215 MNSSGSTGLPKGVALPHRTA luciferase MNSSGSTGLPKGVALPHRTA t g a a c t c c t c t g g a t c t a c t g g t c t g c c t a a a g g t g t c g c t c t g c c t c a t a g a a c t g c c t a c t t g a g g a g a c c t a g a t g a c c a g a c g g a t t t c c a c a g c g a g a c g g a g t a t c t t g a c g g a

4560 220 225 230 235 CVRFSHARDPIFGNQIIPDT luciferase CVRFSHARDPIFGNQIIPDT g c g t g a g a t t c t c g c a t g c c a g a g a t c c t a t t t t t g g c a a t c a a a t c a t t c c g g a t a c t g c g c a c t c t a a g a g c g t a c g g t c t c t a g g a t a a a a a c c g t t a g t t t a g t a a g g c c t a t g a c

4620 240 245 250 255 AILSVVPFHHGFGMFTTLGY luciferase AILSVVPFHHGFGMFTTLGY c g a t t t t a a g t g t t g t t c c a t t c c a t c a c g g t t t t g g a a t g t t t a c t a c a c t c g g a t a t t g c t a a a a t t c a c a a c a a g g t a a g g t a g t g c c a a a a c c t t a c a a a t g a t g t g a g c c t a t a a

4680 260 265 270 275 LICGFRVVLMYRFEEELFLR luciferase LICGFRVVLMYRFEEELFLR t g a t a t g t g g a t t t c g a g t c g t c t t a a t g t a t a g a t t t g a a g a a g a g c t g t t t c t g a g g a a c t a t a c a c c t a a a g c t c a g c a g a a t t a c a t a t c t a a a c t t c t t c t c g a c a a a g a c t c c t

4740 280 285 290 295 SLQDYKIQSALLVPTLFSFF luciferase SLQDYKIQSALLVPTLFSFF g c c t t c a g g a t t a c a a g a t t c a a a g t g c g c t g c t g g t g c c a a c c c t a t t c t c c t t c t t c g c g g a a g t c c t a a t g t t c t a a g t t t c a c g c g a c g a c c a c g g t t g g g a t a a g a g g a a g a a g c

4800 300 305 310 315 AKSTLIDKYDLSNLHEIASG luciferase AKSTLIDKYDLSNLHEIASG c c a a a a g c a c t c t g a t t g a c a a a t a c g a t t t a t c t a a t t t a c a c g a a a t t g c t t c t g g t g g g t t t t c g t g a g a c t a a c t g t t t a t g c t a a a t a g a t t a a a t g t g c t t t a a c g a a g a c c a c

4860 320 325 330 335 GAPLSKEVGEAVAKRFHLPG luciferase GAPLSKEVGEAVAKRFHLPG g c g c t c c c c t c t c t a a g g a a g t c g g g g a a g c g g t t g c c a a g a g g t t c c a t c t g c c a g g t a c g c g a g g g g a g a g a t t c c t t c a g c c c c t t c g c c a a c g g t t c t c c a a g g t a g a c g g t c c a t

4920 340 345 350 355 IRQGYGLTETTSAILITPEG luciferase IRQGYGLTETTSAILITPEG t c a g g c a a g g a t a t g g g c t c a c t g a g a c t a c a t c a g c t a t t c t g a t t a c a c c c g a g g g g g a g t c c g t t c c t a t a c c c g a g t g a c t c t g a t g t a g t c g a t a a g a c t a a t g t g g g c t c c c c c

4980 360 365 370 375 DDKPGAVGKVVPFFEAKVVD luciferase DDKPGAVGKVVPFFEAKVVD a t g a t a a a c c g g g c g c g g t c g g t a a a g t t g t t c c a t t t t t t g a a g c g a a g g t t g t g g a t c t a c t a t t t g g c c c g c g c c a g c c a t t t c a a c a a g g t a a a a a a c t t c g c t t c c a a c a c c t a g

PpuMI 5040 380 385 390 395 LDTGKTLGVNQRGELCVRGP luciferase LDTGKTLGVNQRGELCVRGP t g g a t a c c g g g a a a a c g c t g g g c g t t a a t c a a a g a g g c g a a c t g t g t g t g a g a g g t c c t a a c c t a t g g c c c t t t t g c g a c c c g c a a t t a g t t t c t c c g c t t g a c a c a c a c t c t c c a g g a t

5100 400 405 410 415 MIMSGYVNNPEATNALIDKD luciferase MIMSGYVNNPEATNALIDKD t g a t t a t g t c c g g t t a t g t a a a c a a t c c g g a a g c g a c c a a c g c c t t g a t t g a c a a g g a t g a c t a a t a c a g g c c a a t a c a t t t g t t a g g c c t t c g c t g g t t g c g g a a c t a a c t g t t c c t a c

5160 420 425 430 435 GWLHSGDIAYWDEDEHFFIV luciferase GWLHSGDIAYWDEDEHFFIV g a t g g c t a c a t t c t g g a g a c a t a g c t t a c t g g g a c g a a g a c g a a c a c t t c t t c a t c g t t g c t a c c g a t g t a a g a c c t c t g t a t c g a a t g a c c c t g c t t c t g c t t g t g a a g a a g t a g c a a c

5220 440 445 450 455 DRLKSLIKYKGYQVAPAELE luciferase DRLKSLIKYKGYQVAPAELE a c c g c c t g a a g t c t c t g a t t a a g t a c a a a g g c t a t c a g g t g g c t c c c g c t g a a t t g g a a t t g g c g g a c t t c a g a g a c t a a t t c a t g t t t c c g a t a g t c c a c c g a g g g c g a c t t a a c c t t a

5280 460 465 470 475 SILLQHPNIFDAGVAGLPDD luciferase SILLQHPNIFDAGVAGLPDD c c a t c t t g c t c c a a c a c c c c a a c a t c t t c g a c g c a g g t g t c g c a g g t c t t c c c g a c g a t g g g t a g a a c g a g g t t g t g g g g t t g t a g a a g c t g c g t c c a c a g c g t c c a g a a g g g c t g c t a c

SgrAI 5340 480 485 490 495 DAGELPAAVVVLEHGKTMTE luciferase DAGELPAAVVVLEHGKTMTE a c g c c g g t g a a c t t c c c g c c g c c g t t g t t g t t t t g g a g c a c g g a a a g a c g a t g a c g g a a a t g c g g c c a c t t g a a g g g c g g c g g c a a c a a c a a a a c c t c g t g c c t t t c t g c t a c t g c c t t t

5400 500 505 510 515 KEIVDYVASQVTTAKKLRGG luciferase KEIVDYVASQVTTAKKLRGG a a g a g a t c g t g g a t t a c g t c g c c a g t c a a g t a a c a a c c g c g a a a a a g t t g c g c g g a g g a g t t c t c t a g c a c c t a a t g c a g c g g t c a g t t c a t t g t t g g c g c t t t t t c a a c g c g c c t c c t c

5460 520 525 530 535 VVFVDEVPKGLTGKLDARKI luciferase VVFVDEVPKGLTGKLDARKI t t g t g t t t g t g g a c g a a g t a c c g a a a g g t c t t a c c g g a a a a c t c g a c g c a a g a a a a a t c a a a c a c a a a c a c c t g c t t c a t g g c t t t c c a g a a t g g c c t t t t g a g c t g c g t t c t t t t t a g t

5520 540 545 550 REILIKAKKGGKIAV* luciferase bGH poly(A) signal REILIKAKKGGKIAV* g a g a g a t c c t c a t a a a g g c c a a g a a g g g c g g a a a g a t c g c c g t g t a a g a c t g t g c c t t c t c t c t c t a g g a g t a t t t c c g g t t c t t c c c g c c t t t c t a g c g g c a c a t t c t g a c a c g g a a g a

5580 bGH poly(A) signal a g t t g c c a g c c a t c t g t t g t t t g c c c c t c c c c c g t g c c t t c c t t g a c c c t g g a a g g t g c c t c a a c g g t c g g t a g a c a a c a a a c g g g g a g g g g g c a c g g a a g g a a c t g g g a c c t t c c a c g g

5640 bGH poly(A) signal a c t c c c a c t g t c c t t t c c t a a t a a a a t g a g g a a a t t g c a t c g c a t t g t c t g a g t a g g t g t t g a g g g t g a c a g g a a a g g a t t a t t t t a c t c c t t t a a c g t a g c g t a a c a g a c t c a t c c a c a

5700 bGH poly(A) signal c a t t c t a t t c t g g g g g g t g g g g t g g g g c a g g a c a g c a a g g g g g a g g a t t g g g a a g a c a a t g t a a g a t a a g a c c c c c c a c c c c a c c c c g t c c t g t c g t t c c c c c t c c t a a c c c t t c t g t t a

PvuII 5760 bGH poly(A) signal a g c a g g c a t g c t g g g g a t g c g g t g g g c t c t a t g g c t t c t g a g g c g g a a a g a a c c a g c t g g t c g t c c g t a c g a c c c c t a c g c c a c c c g a g a t a c c g a a g a c t c c g c c t t t c t t g g t c g a c c

5820 g g c t c t a g g g g g t a t c c c c a c g c g c c c t g t a g c g g c g c a t t a a g c g c g g c g g g t g t g g t g c c g a g a t c c c c c a t a g g g g t g c g c g g g a c a t c g c c g c g t a a t t c g c g c c g c c c a c a c c a c

5880 G A G G A A A G C G A A A G F1ori-R g t t a c g c g c a g c g t g a c c g c t a c a c t t g c c a g c g c c c t a g c g c c c g c t c c t t t c g c t t t c c a a t g c g c g t c g c a c t g g c g a t g t g a a c g g t c g c g g g a t c g c g g g c g a g g a a a g c g a a a g

NgoMIVNaeI 5940 A A G G G A F1ori-R t t c c c t t c c t t t c t c g c c a c g t t c g c c g g c t t t c c c c g t c a a g c t c t a a a t c g g g g g c t c a a g g g a a g g a a a g a g c g g t g c a a g c g g c c g a a a g g g g c a g t t c g a g a t t t a g c c c c c g a g

6000 c c t t t a g g g t t c c g a t t t a g t g c t t t a c g g c a c c t c g a c c c c a a a a a a c t t g a t t a g g g t g g a a a t c c c a a g g c t a a a t c a c g a a a t g c c g t g g a g c t g g g g t t t t t t g a a c t a a t c c c a

NotIXbaI 6060 g a t g g t t c a c g t a c c t a g a a g t t c c t a t t c c g a a g t t c c t a t t c t c t a g a g c g g c c g c t t c t a c c a a g t g c a t g g a t c t t c a a g g a t a a g g c t t c a a g g a t a a g a g a t c t c g c c g g c g a a

6120 SV40 poly(A) signal C T A C T C A A A C C T G T T T G G T G EBV-rev c g a g c a g a c a t g a t a a g a t a c a t t g a t g a g t t t g g a c a a a c c a c a a c t a g a a t g c a g t g a g c t c g t c t g t a c t a t t c t a t g t a a c t a c t c a a a c c t g t t t g g t g t t g a t c t t a c g t c a c t

6180 SV40 poly(A) signal G A A A T T T G T G A T G C T A T T G C SV40pA-R a a a a a a t g c t t t a t t t g t g a a a t t t g t g a t g c t a t t g c t t t a t t t g t a a c c a t t a t a a g c t t t t t t a c g a a a t a a a c a c t t t a a a c a c t a c g a t a a c g a a a t a a a c a t t g g t a a t a t t c g

HpaI 6240 SV40 poly(A) signal t g c a a t a a a c a a g t t a a c a a c a a c a a t t g c a t t c a t t t t a t g t t t c a g g t t c a g g g g g a g a c g t t a t t t g t t c a a t t g t t g t t g t t a a c g t a a g t a a a a t a c a a a g t c c a a g t c c c c c t c

BamHIBspDIClaI 6300 g t g t g g g a g g t t t t t t a a a g c a a g t a a a a c c t c t a c a a a t g t g g t a a a a t c g a t a a g g a t c a c a c c c t c c a a a a a a t t t c g t t c a t t t t g g a g a t g t t t a c a c c a t t t t a g c t a t t c c t a

6360 AmpR promoter c c a g g t g g c a c t t t t c g g g g a a a t g t g c g c g g a a c c c c t a t t t g t t t a t t t t t c t a a a t a g g t c c a c c g t g a a a a g c c c c t t t a c a c g c g c c t t g g g g a t a a a c a a a t a a a a a g a t t t a t

SspI 6420 AmpR promoter c a t t c a a a t a t g t a t c c g c t c a t g a g a c a a t a a c c c t g a t a a a t g c t t c a a t a a t a t t g a g t a a g t t t a t a c a t a g g c g a g t a c t c t g t t a t t g g g a c t a t t t a c g a a g t t a t t a t a a c t

6480 1 5 10 15 signal sequence MSIQHFRVALIPFFAA AmpR promoter AmpR MSIQHFRVALIPFFAA a a a a g g a a g a g t a t g a g t a t t c a a c a t t t c c g t g t c g c c c t t a t t c c c t t t t t t g c g g c a t t t t c c t t c t c a t a c t c a t a a g t t g t a a a g g c a c a g c g g g a a t a a g g g a a a a a a c g c c g t

6540 20 signal sequence FCLPVFA 25 30 35 HPETLVKVKDAED AmpR FCLPVFAHPETLVKVKDAED t t t t g c c t t c c t g t t t t t g c t c a c c c a g a a a c g c t g g t g a a a g t a a a a g a t g c t g a a g a t a a a a c g g a a g g a c a a a a a c g a g t g g g t c t t t g c g a c c a c t t t c a t t t t c t a c g a c t t c t a

6600 40 45 50 55 QLGARVGYIELDLNSGKILE AmpR QLGARVGYIELDLNSGKILE c a g t t g g g t g c a c g a g t g g g t t a c a t c g a a c t g g a t c t c a a c a g c g g t a a g a t c c t t g a g g t c a a c c c a c g t g c t c a c c c a a t g t a g c t t g a c c t a g a g t t g t c g c c a t t c t a g g a a c t c

6660 60 65 70 75 SFRPEERFPMMSTFKVLLCG AmpR C G A T A C A C C G Amp-R SFRPEERFPMMSTFKVLLCG a g t t t t c g c c c c g a a g a a c g t t t t c c a a t g a t g a g c a c t t t t a a a g t t c t g c t a t g t g g c t c a a a a g c g g g g c t t c t t g c a a a a g g t t a c t a c t c g t g a a a a t t t c a a g a c g a t a c a c c g

6720 80 85 90 95 AVLSRIDAGQEQLGRRIHYS AmpR C G C C A T A A T A Amp-R AVLSRIDAGQEQLGRRIHYS g c g g t a t t a t c c c g t a t t g a c g c c g g g c a a g a g c a a c t c g g t c g c c g c a t a c a c t a t t c t c g c c a t a a t a g g g c a t a a c t g c g g c c c g t t c t c g t t g a g c c a g c g g c g t a t g t g a t a a g a

6780 100 105 110 115 QNDLVEYSPVTEKHLTDGMT AmpR QNDLVEYSPVTEKHLTDGMT c a g a a t g a c t t g g t t g a g t a c t c a c c a g t c a c a g a a a a g c a t c t t a c g g a t g g c a t g a c a g t c t t a c t g a a c c a a c t c a t g a g t g g t c a g t g t c t t t t c g t a g a a t g c c t a c c g t a c t g t

6840 120 125 130 135 VRELCSAAITMSDNTAANLL AmpR VRELCSAAITMSDNTAANLL g t a a g a g a a t t a t g c a g t g c t g c c a t a a c c a t g a g t g a t a a c a c t g c g g c c a a c t t a c t t c a t t c t c t t a a t a c g t c a c g a c g g t a t t g g t a c t c a c t a t t g t g a c g c c g g t t g a a t g a a

PvuI 6900 140 145 150 155 LTTIGGPKELTAFLHNMGDH AmpR LTTIGGPKELTAFLHNMGDH c t g a c a a c g a t c g g a g g a c c g a a g g a g c t a a c c g c t t t t t t g c a c a a c a t g g g g g a t c a t g a c t g t t g c t a g c c t c c t g g c t t c c t c g a t t g g c g a a a a a a c g t g t t g t a c c c c c t a g t a

6960 160 165 170 175 VTRLDRWEPELNEAIPNDER AmpR VTRLDRWEPELNEAIPNDER g t a a c t c g c c t t g a t c g t t g g g a a c c g g a g c t g a a t g a a g c c a t a c c a a a c g a c g a g c g t c a t t g a g c g g a a c t a g c a a c c c t t g g c c t c g a c t t a c t t c g g t a t g g t t t g c t g c t c g c a

FspI 7020 180 185 190 195 DTTMPVAMATTLRKLLTGEL AmpR DTTMPVAMATTLRKLLTGEL g a c a c c a c g a t g c c t g t a g c a a t g g c a a c a a c g t t g c g c a a a c t a t t a a c t g g c g a a c t a c t g t g g t g c t a c g g a c a t c g t t a c c g t t g t t g c a a c g c g t t t g a t a a t t g a c c g c t t g a t

NmeAIII 7080 200 205 210 215 LTLASRQQLIDWMEADKVAG AmpR LTLASRQQLIDWMEADKVAG c t t a c t c t a g c t t c c c g g c a a c a a t t a a t a g a c t g g a t g g a g g c g g a t a a a g t t g c a g g a g a a t g a g a t c g a a g g g c c g t t g t t a a t t a t c t g a c c t a c c t c c g c c t a t t t c a a c g t c c t

7140 220 225 230 235 PLLRSALPAGWFIADKSGAG AmpR PLLRSALPAGWFIADKSGAG c c a c t t c t g c g c t c g g c c c t t c c g g c t g g c t g g t t t a t t g c t g a t a a a t c t g g a g c c g g t g g t g a a g a c g c g a g c c g g g a a g g c c g a c c g a c c a a a t a a c g a c t a t t t a g a c c t c g g c c a

BsaI 7200 240 245 250 255 ERGSRGIIAALGPDGKPSRI AmpR ERGSRGIIAALGPDGKPSRI g a g c g t g g g t c t c g c g g t a t c a t t g c a g c a c t g g g g c c a g a t g g t a a g c c c t c c c g t a t c c t c g c a c c c a g a g c g c c a t a g t a a c g t c g t g a c c c c g g t c t a c c a t t c g g g a g g g c a t a g

7260 260 265 270 275 VVIYTTGSQATMDERNRQIA AmpR VVIYTTGSQATMDERNRQIA g t a g t t a t c t a c a c g a c g g g g a g t c a g g c a a c t a t g g a t g a a c g a a a t a g a c a g a t c g c t c a t c a a t a g a t g t g c t g c c c c t c a g t c c g t t g a t a c c t a c t t g c t t t a t c t g t c t a g c g a

7320 280 285 EIGASLIKHW* AmpR EIGASLIKHW* g a g a t a g g t g c c t c a c t g a t t a a g c a t t g g t a a c t g t c a g a c c a a g t t t a c t c a t a t a t a c t c t a t c c a c g g a g t g a c t a a t t c g t a a c c a t t g a c a g t c t g g t t c a a a t g a g t a t a t a t

7380 c t t t a g a t t g a t t t a a a a c t t c a t t t t t a a t t t a a a a g g a t c t a g g t g a a g a t c c t t t t t g a a a t c t a a c t a a a t t t t g a a g t a a a a a t t a a a t t t t c c t a g a t c c a c t t c t a g g a a a a a

7440 g a t a a t c t c a t g a c c a a a a t c c c t t a a c g t g a g t t t t c g t t c c a c t g a g c g t c a g a c c c c c t a t t a g a g t a c t g g t t t t a g g g a a t t g c a c t c a a a a g c a a g g t g a c t c g c a g t c t g g g g

7500 ori g t a g a a a a g a t c a a a g g a t c t t c t t g a g a t c c t t t t t t t c t g c g c g t a a t c t g c t g c t t g c a t c t t t t c t a g t t t c c t a g a a g a a c t c t a g g a a a a a a a g a c g c g c a t t a g a c g a c g a a c

7560 ori c a a a c a a a a a a a c c a c c g c t a c c a g c g g t g g t t t g t t t g c c g g a t c a a g a g c t a c c a a c t g t t t g t t t t t t t g g t g g c g a t g g t c g c c a c c a a a c a a a c g g c c t a g t t c t c g a t g g t t g a

7620 ori c t t t t t c c g a a g g t a a c t g g c t t c a g c a g a g c g c a g a t a c c a a a t a c t g t t c t t c t a g t g g a a a a a g g c t t c c a t t g a c c g a a g t c g t c t c g c g t c t a t g g t t t a t g a c a a g a a g a t c a c

7680 ori t a g c c g t a g t t a g g c c a c c a c t t c a a g a a c t c t g t a g c a c c g c c t a c a t a c c t c g c t c t g a t c g g c a t c a a t c c g g t g g t g a a g t t c t t g a g a c a t c g t g g c g g a t g t a t g g a g c g a g a c

7740 ori c t a a t c c t g t t a c c a g t g g c t g c t g c c a g t g g c g a t a a g t c g t g t c t t a c c g g g t t g g a c g a t t a g g a c a a t g g t c a c c g a c g a c g g t c a c c g c t a t t c a g c a c a g a a t g g c c c a a c c t g

7800 ori t c a a g a c g a t a g t t a c c g g a t a a g g c g c a g c g g t c g g g c t g a a c g g g g g g t t c g t g c a c a a g t t c t g c t a t c a a t g g c c t a t t c c g c g t c g c c a g c c c g a c t t g c c c c c c a a g c a c g t g t

7860 ori c a g c c c a g c t t g g a g c g a a c g a c c t a c a c c g a a c t g a g a t a c c t a c a g c g t g a g c t a t g a g t c g g g t c g a a c c t c g c t t g c t g g a t g t g g c t t g a c t c t a t g g a t g t c g c a c t c g a t a c t

7920 ori g a a a g c g c c a c g c t t c c c g a a g g g a g a a a g g c g g a c a g g t a t c c g g t a a g c g g c a g g g t c c t t t c g c g g t g c g a a g g g c t t c c c t c t t t c c g c c t g t c c a t a g g c c a t t c g c c g t c c c a g

7980 ori G G G A A A C G C C T G G T A T C T T T pBR322ori-F g g a a c a g g a g a g c g c a c g a g g g a g c t t c c a g g g g g a a a c g c c t g g t a t c t t t a t a g t c c t c c t t g t c c t c t c g c g t g c t c c c t c g a a g g t c c c c c t t t g c g g a c c a t a g a a a t a t c a g g a

8040 ori g t c g g g t t t c g c c a c c t c t g a c t t g a g c g t c g a t t t t t g t g a t g c t c g t c a g g g g g g c g g c a g c c c a a a g c g g t g g a g a c t g a a c t c g c a g c t a a a a a c a c t a c g a g c a g t c c c c c c g c c

8100 ori a g c c t a t g g a a a a a c g c c a g c a a c g c g g c c t t t t t a c g g t t c c t g g c c t t t t g c t g g c c t t c g g a t a c c t t t t t g c g g t c g t t g c g c c g g a a a a a t g c c a a g g a c c g g a a a a c g a c c g g a

BglII 8160 A T G T T A G A C pRS-marker t t t g c t c a c a t g g c t c g a c a g a t c t c c c g a t c c c c t a t g g t g c a c t c t c a g t a c a a t c t g a a a c g a g t g t a c c g a g c t g t c t a g a g g g c t a g g g g a t a c c a c g t g a g a g t c a t g t t a g a c

8220 G A G A C T A C G G C pRS-marker c t c t g a t g c c g c a t a g t t a a g c c a g t a t c t g c t c c c t g c t t g t g t g t t g g a g g t c g c t g a g a g a c t a c g g c g t a t c a a t t c g g t c a t a g a c g a g g g a c g a a c a c a c a a c c t c c a g c g a c t

8280 g t a g t g c g c g a g c a a a a t t t a a g c t a c a a c a a g g c a a g g c t t g a c c g a c a a t t g c a t g a a c a t c a c g c g c t c g t t t t a a a t t c g a t g t t g t t c c g t t c c g a a c t g g c t g t t a a c g t a c t t

MluINruIBpu10I 8340 g a a t c t g c t t a g g g t t a g g c g t t t t g c g c t g c t t c g c g a t g t a c g g g c c a g a t a t a c g c g c t t a g a c g a a t c c c a a t c c g c a a a a c g c g a c g a a g c g c t a c a t g c c c g g t c t a t a t g c g c

SpeI 8400 CMV enhancer t t g a c a t t g a t t a t t g a c t a g t t a t t a a t a g t a a t c a a t t a c g g g g t c a t t a g t t c a t a g a a c t g t a a c t a a t a a c t g a t c a a t a a t t a t c a t t a g t t a a t g c c c c a g t a a t c a a g t a t c

8460 CMV enhancer c c c a t a t a t g g a g t t c c g c g t t a c a t a a c t t a c g g t a a a t g g c c c g c c t g g c t g a c c g c c g g g t a t a t a c c t c a a g g c g c a a t g t a t t g a a t g c c a t t t a c c g g g c g g a c c g a c t g g c g g

8520 CMV enhancer c a a c g a c c c c c g c c c a t t g a c g t c a a t a a t g a c g t a t g t t c c c a t a g t a a c g c c a a t a g g g t t g c t g g g g g c g g g t a a c t g c a g t t a t t a c t g c a t a c a a g g g t a t c a t t g c g g t t a t c c

8580 CMV enhancer g a c t t t c c a t t g a c g t c a a t g g g t g g a g t a t t t a c g g t a a a c t g c c c a c t t g g c a g t a c a c t g a a a g g t a a c t g c a g t t a c c c a c c t c a t a a a t g c c a t t t g a c g g g t g a a c c g t c a t g t

NdeI 8640 CMV enhancer t c a a g t g t a t c a t a t g c c a a g t a c g c c c c c t a t t g a c g t c a a t g a c g g t a a a t g g c c c g c a g t t c a c a t a g t a t a c g g t t c a t g c g g g g g a t a a c t g c a g t t a c t g c c a t t t a c c g g g c g

SnaBI 8700 CMV enhancer c t g g c a t t a t g c c c a g t a c a t g a c c t t a t g g g a c t t t c c t a c t t g g c a g t a c a t c t a c g t g a c c g t a a t a c g g g t c a t g t a c t g g a a t a c c c t g a a a g g a t g a a c c g t c a t g t a g a t g c a

8760 CMV enhancer CMV promoter a t t a g t c a t c g c t a t t a c c a t g g t g a t g c g g t t t t g g c a g t a c a t c a a t g g g c g t g g a t a t a a t c a g t a g c g a t a a t g g t a c c a c t a c g c c a a a a c c g t c a t g t a g t t a c c c g c a c c t a t

8820 CMV promoter g c g g t t t g a c t c a c g g g g a t t t c c a a g t c t c c a c c c c a t t g a c g t c a a t g g g a g t t t g t t c g c c a a a c t g a g t g c c c c t a a a g g t t c a g a g g t g g g g t a a c t g c a g t t a c c c t c a a a c a a

8880 CMV promoter C G C A CMV-F t t g g c a c c a a a a t c a a c g g g a c t t t c c a a a a t g t c g t a a c a a c t c c g c c c c a t t g a c g c a a a c c g t g g t t t t a g t t g c c c t g a a a g g t t t t a c a g c a t t g t t g a g g c g g g g t a a c t g c g t

8940 CMV promoter A A T G G G C G G T A G G C G T G CMV-F A G C T C G T T T A G T G A A C C G LNCX a a t g g g c g g t a g g c g t g t a c g g t g g g a g g t c t a t a t a a g c a g a g c t c g t t t a g t g a a c c g t t a c c c g c c a t c c g c a c a t g c c a c c c t c c a g a t a t a t t c g t c t c g a g c a a a t c a c t t g g c

9000 T C A G A T C LNCX t c a g a t c g c c t g g a g a c g c c a t c c a c g c t g t t t t g a c c t c c a t a g a a g a c a c c g g g a c c g a g t c t a g c g g a c c t c t g c g g t a g g t g c g a c a a a a c t g g a g g t a t c t t c t g t g g c c c t g g c

SbfI 9060 a t c c a g c c t c c g c g g c c g g g a a c g g t g c a t t g g a a c g g a c c t g c a g g a a t t g a t c c g c g g t a g g t c g g a g g c g c c g g c c c t t g c c a c g t a a c c t t g c c t g g a c g t c c t t a a c t a g g c g c c

3ʹ 5ʹ 9086 c c c t a g c g c t a c c g g a c t c a g a t c c a g g g a t c g c g a t g g c c t g a g t c t a g g t

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.