Addgene pHH0103_SH3PXD2A-2/5 Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: pHH0103_SH3PXD2A-2/5 Sequencing Result


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AatII (6886) ZraI (6884) Amp-R (6514 .. 6533) PvuI (6334) PstI (6209) BsaI (6025) AhdI (5964) AlwNI (5487) L4440 (4962 .. 4979) pRS-marker (4862 .. 4881) BstZ17I (4845) AccI (4844) PflFI - Tth111I (4819) pGEX 3' (4699 .. 4721) PfoI (4716) F1ori-R (4551 .. 4570) DraIII (4426) F1ori-F (4339 .. 4360) PsiI (4298) AfeI (3856) BsaBI * (3799) BspEI * (3791) Bpu10I (3708) FspAI (3583) AvaI - BsoBI (3552) pBRforEco (12 .. 30) EcoRI (45) lacIq promoter LacI-R (150 .. 169) MluI (481) BstEII (662) EcoRV (931) HpaI (987) KasI (1120) NarI * (1121) SfoI (1122) PluTI (1124) BfuAI - BspMI (1335) M13/pUC Reverse (1497 .. 1519) lac operator HindIII (1620) EcoNI (1636) BstBI (2023) SwaI (2053) pGEX 5' (2237 .. 2259) thrombin site Acc65I (2340) KpnI (2344) SacII (2347) SfiI (2355) NcoI (2359) PasI (2366) EagI - NotI (2548) BspDI - ClaI (2986) NheI (3329) BmtI (3333) pHH0103_SH3PXD2A-2/5 6912 bp

Sequence View

EcoRI 5ʹ 3ʹ 60 lacIq promoter A A T A G G C G T A T C A C G A G G C pBRforEco t a a c c t a t a a a a a t a g g c g t a t c a c g a g g c c c t t t c g t c t t c a a g a a t t c c c g a c a c c a t a t t g g a t a t t t t t a t c c g c a t a g t g c t c c g g g a a a g c a g a a g t t c t t a a g g g c t g t g g t a

120 lacIq promoter c g a a t g g t g c a a a a c c t t t c g c g g t a t g g c a t g a t a g c g c c c g g a a g a g a g t c a a t t c a g g c t t a c c a c g t t t t g g a a a g c g c c a t a c c g t a c t a t c g c g g g c c t t c t c t c a g t t a a g t c

180 1 5 10 15 MKPVTLYDVAEYAGVSY lacIq promoter lacI T G C T A C A G C G T C T C A T A C G G LacI-R g g t g g t g a a t g t g a a a c c a g t a a c g t t a t a c g a t g t c g c a g a g t a t g c c g g t g t c t c t t a c c a c c a c t t a c a c t t t g g t c a t t g c a a t a t g c t a c a g c g t c t c a t a c g g c c a c a g a g a a t

240 20 25 30 35 QTVSRVVNQASHVSAKTREK lacI t c a g a c c g t t t c c c g c g t g g t g a a c c a g g c c a g c c a c g t t t c t g c g a a a a c g c g g g a a a a a g t c t g g c a a a g g g c g c a c c a c t t g g t c c g g t c g g t g c a a a g a c g c t t t t g c g c c c t t t t

300 40 45 50 55 VEAAMAELNYIPNRVAQQLA lacI MAELNYIPNRVAQQLA a g t g g a a g c g g c g a t g g c g g a g c t g a a t t a c a t t c c c a a c c g c g t g g c a c a a c a a c t g g c t c a c c t t c g c c g c t a c c g c c t c g a c t t a a t g t a a g g g t t g g c g c a c c g t g t t g t t g a c c g

360 60 65 70 75 GKQSLLIGVATSSLALHAPS lacI GKQSLLIGVATSSLALHAPS g g g c a a a c a g t c g t t g c t g a t t g g c g t t g c c a c c t c c a g t c t g g c c c t g c a c g c g c c g t c c c c g t t t g t c a g c a a c g a c t a a c c g c a a c g g t g g a g g t c a g a c c g g g a c g t g c g c g g c a g

420 80 85 90 95 QIVAAIKSRADQLGASVVVS lacI QIVAAIKSRADQLGASVVVS g c a a a t t g t c g c g g c g a t t a a a t c t c g c g c c g a t c a a c t g g g t g c c a g c g t g g t g g t g t c c g t t t a a c a g c g c c g c t a a t t t a g a g c g c g g c t a g t t g a c c c a c g g t c g c a c c a c c a c a g

480 100 105 110 115 MVERSGVEACKAAVHNLLAQ lacI MVERSGVEACKAAVHNLLAQ g a t g g t a g a a c g a a g c g g c g t c g a a g c c t g t a a a g c g g c g g t g c a c a a t c t t c t c g c g c a c t a c c a t c t t g c t t c g c c g c a g c t t c g g a c a t t t c g c c g c c a c g t g t t a g a a g a g c g c g t

MluI 540 120 125 130 135 RVSGLIINYPLDDQDAIAVE lacI RVSGLIINYPLDDQDAIAVE a c g c g t c a g t g g g c t g a t c a t t a a c t a t c c g c t g g a t g a c c a g g a t g c c a t t g c t g t g g a t g c g c a g t c a c c c g a c t a g t a a t t g a t a g g c g a c c t a c t g g t c c t a c g g t a a c g a c a c c t

600 140 145 150 155 AACTNVPALFLDVSDQTPIN lacI AACTNVPALFLDVSDQTPIN a g c t g c c t g c a c t a a t g t t c c g g c g t t a t t t c t t g a t g t c t c t g a c c a g a c a c c c a t c a a t c g a c g g a c g t g a t t a c a a g g c c g c a a t a a a g a a c t a c a g a g a c t g g t c t g t g g g t a g t t

660 160 165 170 175 SIIFSHEDGTRLGVEHLVAL lacI SIIFSHEDGTRLGVEHLVAL c a g t a t t a t t t t c t c c c a t g a a g a c g g t a c g c g a c t g g g c g t g g a g c a t c t g g t c g c a t t g t c a t a a t a a a a g a g g g t a c t t c t g c c a t g c g c t g a c c c g c a c c t c g t a g a c c a g c g t a a

BstEII 720 180 185 190 195 GHQQIALLAGPLSSVSARLR lacI GHQQIALLAGPLSSVSARLR g g g t c a c c a g c a a a t c g c g c t g t t a g c g g g c c c a t t a a g t t c t g t c t c g g c g c g t c t g c g c c c a g t g g t c g t t t a g c g c g a c a a t c g c c c g g g t a a t t c a a g a c a g a g c c g c g c a g a c g c

780 200 205 210 215 LAGWHKYLTRNQIQPIAERE lacI LAGWHKYLTRNQIQPIAERE t c t g g c t g g c t g g c a t a a a t a t c t c a c t c g c a a t c a a a t t c a g c c g a t a g c g g a a c g g g a a g a c c g a c c g a c c g t a t t t a t a g a g t g a g c g t t a g t t t a a g t c g g c t a t c g c c t t g c c c t

840 220 225 230 235 GDWSAMSGFQQTMQMLNEGI lacI GDWSAMSGFQQTMQMLNEGI a g g c g a c t g g a g t g c c a t g t c c g g t t t t c a a c a a a c c a t g c a a a t g c t g a a t g a g g g c a t t c c g c t g a c c t c a c g g t a c a g g c c a a a a g t t g t t t g g t a c g t t t a c g a c t t a c t c c c g t a

900 240 245 250 255 VPTAMLVANDQMALGAMRAI lacI VPTAMLVANDQMALGAMRAI c g t t c c c a c t g c g a t g c t g g t t g c c a a c g a t c a g a t g g c g c t g g g c g c a a t g c g c g c c a t g c a a g g g t g a c g c t a c g a c c a a c g g t t g c t a g t c t a c c g c g a c c c g c g t t a c g c g c g g t a

EcoRV 960 260 265 270 275 TESGLRVGADISVVGYDDTE lacI TESGLRVGADISVVGYDDTE t a c c g a g t c c g g g c t g c g c g t t g g t g c g g a t a t c t c g g t a g t g g g a t a c g a c g a t a c c g a a t g g c t c a g g c c c g a c g c g c a a c c a c g c c t a t a g a g c c a t c a c c c t a t g c t g c t a t g g c t

HpaI 1020 280 285 290 295 DSSCYIPPLTTIKQDFRLLG lacI DSSCYIPPLTTIKQDFRLLG a g a c a g c t c a t g t t a t a t c c c g c c g t t a a c c a c c a t c a a a c a g g a t t t t c g c c t g c t g g g t c t g t c g a g t a c a a t a t a g g g c g g c a a t t g g t g g t a g t t t g t c c t a a a a g c g g a c g a c c c

1080 300 305 310 315 QTSVDRLLQLSQGQAVKGNQ lacI QTSVDRLLQLSQGQAVKGNQ g c a a a c c a g c g t g g a c c g c t t g c t g c a a c t c t c t c a g g g c c a g g c g g t g a a g g g c a a t c a c g t t t g g t c g c a c c t g g c g a a c g a c g t t g a g a g a g t c c c g g t c c g c c a c t t c c c g t t a g t

KasINarI*SfoIPluTI 1140 320 325 330 335 LLPVSLVKRKTTLAPNTQTA lacI LLPVSLVKRKTTLAPNTQTA g c t g t t g c c c g t c t c a c t g g t g a a a a g a a a a a c c a c c c t g g c g c c c a a t a c g c a a a c c g c c g a c a a c g g g c a g a g t g a c c a c t t t t c t t t t t g g t g g g a c c g c g g g t t a t g c g t t t g g c g

1200 340 345 350 355 SPRALADSLMQLARQVSRLE lacI SPRALADSLMQLARQVSRLE c t c t c c c c g c g c g t t g g c c g a t t c a t t a a t g c a g c t g g c a c g a c a g g t t t c c c g a c t g g a g a g a g g g g c g c g c a a c c g g c t a a g t a a t t a c g t c g a c c g t g c t g t c c a a a g g g c t g a c c t

1260 360 SGQ* lacI SGQ* a a g c g g g c a g t g a g c g c a a c g c a a t t a a t g t a a g t t a g c t c a c t c a t t a g g c a c a a t t c t t t c g c c c g t c a c t c g c g t t g c g t t a a t t a c a t t c a a t c g a g t g a g t a a t c c g t g t t a a g a

1320 c a t g t t t g a c a g c t t a t c a t c g a c t g c a c g g t g c a c c a a t g c t t c t g g c g t c a g g c a g c c g t a c a a a c t g t c g a a t a g t a g c t g a c g t g c c a c g t g g t t a c g a a g a c c g c a g t c c g t c g g

BspMIBfuAI 1380 a t c g g a a g c t g t g g t a t g g c t g t g c a g g t c g t a a a t c a c t g c a t a a t t c g t g t c g c t c a a t a g c c t t c g a c a c c a t a c c g a c a c g t c c a g c a t t t a g t g a c g t a t t a a g c a c a g c g a g t t

1440 g g c g c a c t c c c g t t c t g g a t a a t g t t t t t t g c g c c g a c a t c a t a a c g g t t c t g g c a a a t a c c g c g t g a g g g c a a g a c c t a t t a c a a a a a a c g c g g c t g t a g t a t t g c c a a g a c c g t t t a t

1500 -35 -10 tac promoter lac operator A G C G M13/pUC Reverse t t c t g a a a t g a g c t g t t g a c a a t t a a t c a t c g g c t c g t a t a a t g t g t g g a a t t g t g a g c g a a g a c t t t a c t c g a c a a c t g t t a a t t a g t a g c c g a g c a t a t t a c a c a c c t t a a c a c t c g c

1560 lac operator G A T A A C A A T T T C A C A C A G G M13/pUC Reverse g a t a a c a a t t t c a c a c a g g a a a c a g c c a g t c c g t t t a g g t g t t t t c a c g a g c a c t t c a c c c t a t t g t t a a a g t g t g t c c t t t g t c g g t c a g g c a a a t c c a c a a a a g t g c t c g t g a a g t g g

1620 1 5 HHHHHH SSG 6xHis MKIEEHHHHHHSSG a a c a a g g a c c a t a g a t t a t g a a a a t c g a a g a a c a c c a t c a c c a t c a c c a t t c c a g c g g t a t t g t t c c t g g t a t c t a a t a c t t t t a g c t t c t t g t g g t a g t g g t a g t g g t a a g g t c g c c a t

EcoNIHindIII 1680 KL 1 5 10 15 MSPILGYWKIKGLVQPTR GST KLMSPILGYWKIKGLVQPTR a g c t t a t g t c c c c t a t a c t a g g t t a t t g g a a a a t t a a g g g c c t t g t g c a a c c c a c t c g a c t c g a a t a c a g g g g a t a t g a t c c a a t a a c c t t t t a a t t c c c g g a a c a c g t t g g g t g a g c t g

1740 20 25 30 35 LLLEYLEEKYEEHLYERDEG GST LLLEYLEEKYEEHLYERDEG t t c t t t t g g a a t a t c t t g a a g a a a a a t a t g a a g a g c a t t t g t a t g a g c g c g a t g a a g g t g a a g a a a a c c t t a t a g a a c t t c t t t t t a t a c t t c t c g t a a a c a t a c t c g c g c t a c t t c c a c

1800 40 45 50 55 DKWRNKKFELGLEFPNLPYY GST DKWRNKKFELGLEFPNLPYY a t a a a t g g c g a a a c a a a a a g t t t g a a t t g g g t t t g g a g t t t c c c a a t c t t c c t t a t t a t a t a t t t a c c g c t t t g t t t t t c a a a c t t a a c c c a a a c c t c a a a g g g t t a g a a g g a a t a a t a t

1860 60 65 70 75 IDGDVKLTQSMAIIRYIADK GST IDGDVKLTQSMAIIRYIADK t t g a t g g t g a t g t t a a a t t a a c a c a g t c t a t g g c c a t c a t a c g t t a t a t a g c t g a c a a g c a a c t a c c a c t a c a a t t t a a t t g t g t c a g a t a c c g g t a g t a t g c a a t a t a t c g a c t g t t c g

1920 80 85 90 95 HNMLGGCPKERAEISMLEGA GST HNMLGGCPKERAEISMLEGA a c a a c a t g t t g g g t g g t t g t c c a a a a g a g c g t g c a g a g a t t t c a a t g c t t g a a g g a g c g g t g t t g t a c a a c c c a c c a a c a g g t t t t c t c g c a c g t c t c t a a a g t t a c g a a c t t c c t c g c c

1980 100 105 110 115 VLDIRYGVSRIAYSKDFETL GST VLDIRYGVSRIAYSKDFETL t t t t g g a t a t t a g a t a c g g t g t t t c g a g a a t t g c a t a t a g t a a a g a c t t t g a a a c t c t c a a a a a c c t a t a a t c t a t g c c a c a a a g c t c t t a a c g t a t a t c a t t t c t g a a a c t t t g a g a g t

BstBI 2040 120 125 130 135 KVDFLSKLPEMLKMFEDRLC GST KVDFLSKLPEMLKMFEDRLC a a g t t g a t t t t c t t a g c a a g c t a c c t g a a a t g c t g a a a a t g t t c g a a g a t c g t t t a t g t c t t c a a c t a a a a g a a t c g t t c g a t g g a c t t t a c g a c t t t t a c a a g c t t c t a g c a a a t a c a g

SwaI 2100 140 145 150 155 HKTYLNGDHVTHPDFMLYDA GST HKTYLNGDHVTHPDFMLYDA a t a a a a c a t a t t t a a a t g g t g a t c a t g t a a c c c a t c c t g a c t t c a t g t t g t a t g a c g c t c t a t t t t g t a t a a a t t t a c c a c t a g t a c a t t g g g t a g g a c t g a a g t a c a a c a t a c t g c g a g

2160 160 165 170 175 LDVVLYMDPMCLDAFPKLVC GST LDVVLYMDPMCLDAFPKLVC t t g a t g t t g t t t t a t a c a t g g a c c c a a t g t g c c t g g a t g c g t t c c c a a a a t t a g t t t g t t a a c t a c a a c a a a a t a t g t a c c t g g g t t a c a c g g a c c t a c g c a a g g g t t t t a a t c a a a c a a

2220 180 185 190 195 FKKRIEAIPQIDKYLKSSKY GST FKKRIEAIPQIDKYLKSSKY t t a a a a a a c g t a t t g a a g c t a t c c c a c a a a t t g a t a a g t a c t t g a a a t c c a g c a a g t a t a a a t t t t t t g c a t a a c t t c g a t a g g g t g t t t a a c t a t t c a t g a a c t t t a g g t c g t t c a t a t

2280 200 205 210 215 IAWPLQGWQATFGGGDHPPK GST G G G C T G G C A A G C C A C G T T T G G T G pGEX 5' IAWPLQGWQATFGGGDHPPK t a g c a t g g c c t t t g c a g g g c t g g c a a g c c a c g t t t g g t g g t g g c g a c c a t c c t c c a a a a t a t c g t a c c g g a a a c g t c c c g a c c g t t c g g t g c a a a c c a c c a c c g c t g g t a g g a g g t t t t a

2340 SD 1 5 LEVLFQGP LSSG 1 5 LVPRGS HRV 3C site thrombin site SDLEVLFQGPLSSGLVPRGS c g g a t c t g g a a g t t c t g t t c c a g g g g c c c c t g t c c a g c g g t c t g g t t c c g c g t g g t t c c g g c c t a g a c c t t c a a g a c a a g g t c c c c g g g g a c a g g t c g c c a g a c c a a g g c g c a c c a a g g c

PasINcoISfiIAcc65IKpnISacII 2400 (in frame with thrombin site) GTAAQPAMAQGALEKYVTVQ GTAAQPAMAQGALEKYVTVQ g t a c c g c g g c c c a g c c g g c c a t g g c c c a g g g c g c g c t g g a a a a a t a c g t t a c c g t c c a g c c a t g g c g c c g g g t c g g c c g g t a c c g g g t c c c g c g c g a c c t t t t t a t g c a a t g g c a g g t c g

2460 (in frame with thrombin site) PYTSQSKDEIGFEKGVTVEV PYTSQSKDEIGFEKGVTVEV c a t a t a c c t c c c a g t c c a a a g a t g a a a t t g g t t t t g a a a a a g g t g t c a c c g t t g a a g t c a g t a t a t g g a g g g t c a g g t t t c t a c t t t a a c c a a a a c t t t t t c c a c a g t g g c a a c t t c a g t

2520 (in frame with thrombin site) IRKNLEGWWYIRYLGKEGWA IRKNLEGWWYIRYLGKEGWA t t c g t a a a a a c c t g g a a g g t t g g t g g t a c a t t c g t t a c c t g g g t a a a g a a g g t t g g g c a c a a g c a t t t t t g g a c c t t c c a a c c a c c a t g t a a g c a a t g g a c c c a t t t c t t c c a a c c c g t g

EagINotI 2580 (in frame with thrombin site) PASYLKKAKAAA* fd terminator PASYLKKAKAAA* c a g c a t c c t a t c t g a a a a a a g c c a a a g c g g c c g c a t a a t a a a c c g a t a c a a t t a a a g g c t g t c g t a g g a t a g a c t t t t t t c g g t t t c g c c g g c g t a t t a t t t g g c t a t g t t a a t t t c c g a

2640 fd terminator c c t t t t g g a g c c t t t t t t t t g g a g a t t t t c a a c g t g a a a a a a t t a t t a t t c g c a a t t c c t g g a a a a c c t c g g a a a a a a a a c c t c t a a a a g t t g c a c t t t t t t a a t a a t a a g c g t t a a g g a

2700 t t a g t t g t t c c t t t c t a t t c t c a c t c c g c t g a a a c t g t t g a a a g t t g t t t a g c a a a a c c c a a t c a a c a a g g a a a g a t a a g a g t g a g g c g a c t t t g a c a a c t t t c a a c a a a t c g t t t t g g g

2760 c a t a c a g a a a a t t c a t t t a c t a a c g t c t g g a a a g a c g a c a a a a c t t t a g a t c g t t a c g c t g t a t g t c t t t t a a g t a a a t g a t t g c a g a c c t t t c t g c t g t t t t g a a a t c t a g c a a t g c g a

2820 a a c t a t g a g g g c t g t c t g t g g a a t g c t a c a g g c g t t g t a g t t t g t a c t g g t g a c g a a a c t t t g a t a c t c c c g a c a g a c a c c t t a c g a t g t c c g c a a c a t c a a a c a t g a c c a c t g c t t t g a

2880 c a g t g t c t a g c t a g a g t g g c g g t g g c t c t g g t t c c g g t g a t t t t g a t t a t g a a a a g a t g g g t c a c a g a t c g a t c t c a c c g c c a c c g a g a c c a a g g c c a c t a a a a c t a a t a c t t t t c t a c c

2940 c a a a c g c t a a t a a g g g g g c t a t g a c c g a a a a t g c c g a t g a a a a c g c g c t a c a g t c t g a c g g t t t g c g a t t a t t c c c c c g a t a c t g g c t t t t a c g g c t a c t t t t g c g c g a t g t c a g a c t g c

BspDIClaI 3000 c t a a a g g c a a a c t t g a t t c t g t c g c t a c t g a t t a c g g t g c t g c t a t c g a t g g t t t c a t t g g a t t t c c g t t t g a a c t a a g a c a g c g a t g a c t a a t g c c a c g a c g a t a g c t a c c a a a g t a a c

3060 g t g a c g t t t c c g g c c t t g c t a a t g g t a a t g g t g c t a c t g g t g a t t t t g c t g g c t c t a a t t c a c t g c a a a g g c c g g a a c g a t t a c c a t t a c c a c g a t g a c c a c t a a a a c g a c c g a g a t t a a

3120 c c c a a a t g g c t c a a g t c g g t g a c g g t g a t a a t t c a c c t t t a a t g a a t a a t t t c c g t c a a t g g g t t t a c c g a g t t c a g c c a c t g c c a c t a t t a a g t g g a a a t t a c t t a t t a a a g g c a g t t a

3180 a t t t a c c t t c c c t c c c t c a a t c g g t t g a a t g t c g c c c t t t t g t c t t t g g c g c t g g t a a a c t a a a t g g a a g g g a g g g a g t t a g c c a a c t t a c a g c g g g a a a a c a g a a a c c g c g a c c a t t t g

3240 c a t a t g a a t t t t c t a t t g a t t g t g a c a a a a t a a a c t t a t t c c g t g g t g t c t t t g c g t t t c g t a t a c t t a a a a g a t a a c t a a c a c t g t t t t a t t t g a a t a a g g c a c c a c a g a a a c g c a a a g

3300 t t t t a t a t g t t g c c a c c t t t a t g t a t g t a t t t t c t a c g t t t g c t a a c a t a c t g c g t a a t a a a a a t a t a c a a c g g t g g a a a t a c a t a c a t a a a a g a t g c a a a c g a t t g t a t g a c g c a t t a t

BmtINheI 3360 a g g a g t c t t a a t c a t g c c a g t t c t t t t g g c t a g c g c c g c c c t a t a c c t t g t c t g c c t c c c t c c t c a g a a t t a g t a c g g t c a a g a a a a c c g a t c g c g g c g g g a t a t g g a a c a g a c g g a g g g

3420 c g c g t t g c g t c g c g g t g c a t g g a g c c g g g c c a c c t c g a c c t g a a t g g a a g c c g g c g g c a c g c g c a a c g c a g c g c c a c g t a c c t c g g c c c g g t g g a g c t g g a c t t a c c t t c g g c c g c c g t g

3480 c t c g c t a a c g g a t t c a c c a c t c c a a g a a t t g g a g c c a a t c a a t t c t t g c g g a g a a c t g t g g a g c g a t t g c c t a a g t g g t g a g g t t c t t a a c c t c g g t t a g t t a a g a a c g c c t c t t g a c a c

3540 a a t g c g c a a a c c a a c c c t t g g c a g a a c a t a t c c a t c g c g t c c g c c a t c t c c a g c a g c c g c t t a c g c g t t t g g t t g g g a a c c g t c t t g t a t a g g t a g c g c a g g c g g t a g a g g t c g t c g g c g

FspAIBsoBIAvaI 3600 a c g c g g c g c a t c t c g g g c a g c g t t g g g t c c t g g c c a c g g g t g c g c a t g a t c g t g c t c c t g t g c g c c g c g t a g a g c c c g t c g c a a c c c a g g a c c g g t g c c c a c g c g t a c t a g c a c g a g g a c

3660 t c g t t g a g g a c c c g g c t a g g c t g g c g g g g t t g c c t t a c t g g t t a g c a g a a t g a a t c a c c g a g c a a c t c c t g g g c c g a t c c g a c c g c c c c a a c g g a a t g a c c a a t c g t c t t a c t t a g t g g c

Bpu10I 3720 a t a c g c g a g c g a a c g t g a a g c g a c t g c t g c t g c a a a a c g t c t g c g a c c t g a g c a a c a a c a t a t g c g c t c g c t t g c a c t t c g c t g a c g a c g a c g t t t t g c a g a c g c t g g a c t c g t t g t t g t

3780 t g a a t g g t c t t c g g t t t c c g t g t t t c g t a a a g t c t g g a a a c g c g g a a g t c a g c g c c c t g c a c t t a c c a g a a g c c a a a g g c a c a a a g c a t t t c a g a c c t t t g c g c c t t c a g t c g c g g g a c g

BsaBI*BspEI* 3840 a c c a t t a t g t t c c g g a t c t g c a t c g c a g g a t g c t g c t g g c t a c c c t g t g g a a c a c c t a c a t g g t a a t a c a a g g c c t a g a c g t a g c g t c c t a c g a c g a c c g a t g g g a c a c c t t g t g g a t g t

AfeI 3900 t c t g t a t t a a c g a a g c g c t g g c a t t g a c c c t g a g t g a t t t t t c t c t g g t c c c g c c g c a t c a g a c a t a a t t g c t t c g c g a c c g t a a c t g g g a c t c a c t a a a a a g a g a c c a g g g c g g c g t a g

3960 c a t a c c g c c a g t t g t t t a c c c t c a c a a c g t t c c a g t a a c c g g g c a t g t t c a t c a t c a g t a g t a t g g c g g t c a a c a a a t g g g a g t g t t g c a a g g t c a t t g g c c c g t a c a a g t a g t a g t c a t

4020 a c c c g t a t c g t g a g c a t c c t c t c t c g t t t c a t c g g t a t c a t t a c c c c c a t g a a c a g a a a t t g g g c a t a g c a c t c g t a g g a g a g a g c a a a g t a g c c a t a g t a a t g g g g g t a c t t g t c t t t a

4080 c c c c c t t a c a c g g a g g c a t c a g t g a c c a a a c a g g a a a a a a c c g c c c t t a a c a t g g c c c g c g g g g g a a t g t g c c t c c g t a g t c a c t g g t t t g t c c t t t t t t g g c g g g a a t t g t a c c g g g c g

4140 t t t a t c a g a a g c c a g a c a t t a a c g c t t c t g g a g a a a c t c a a c g a g c t g g a c g c g g a t g a a a a a t a g t c t t c g g t c t g t a a t t g c g a a g a c c t c t t t g a g t t g c t c g a c c t g c g c c t a c t t

4200 c a g g c a g a c a t c t g t g a a t c g c t t c a c g a c c a c g c t g a t g a g c t t t a c c g c a g g a t c c g c g t c c g t c t g t a g a c a c t t a g c g a a g t g c t g g t g c g a c t a c t c g a a a t g g c g t c c t a g g c g

4260 f1 ori g a a a t t g t a a g c g t t a a t a t t t t g t t a a a a t t c g c g t t a a a t t t t t g t t a a a t c a g c t c a c t t t a a c a t t c g c a a t t a t a a a a c a a t t t t a a g c g c a a t t t a a a a a c a a t t t a g t c g a g t

PsiI 4320 f1 ori t t t t t t a a c c a a t a g g c c g a a a t c g g c a a a a t c c c t t a t a a a t c a a a a g a a t a g a c c g a g a a a a a a t t g g t t a t c c g g c t t t a g c c g t t t t a g g g a a t a t t t a g t t t t c t t a t c t g g c t c

4380 f1 ori G G T C A A A C C T T G T T C T C A G G T G F1ori-F a t a g g g t t g a g t g t t g t t c c a g t t t g g a a c a a g a g t c c a c t a t t a a a g a a c g t g g a c t c c t a t c c c a a c t c a c a a c a a g g t c a a a c c t t g t t c t c a g g t g a t a a t t t c t t g c a c c t g a g g

DraIII 4440 f1 ori a a c g t c a a a g g g c g a a a a a c c g t c t a t c a g g g c g a t g g c c c a c t a c g t g a a c c a t c a c c c t t g c a g t t t c c c g c t t t t t g g c a g a t a g t c c c g c t a c c g g g t g a t g c a c t t g g t a g t g g g

4500 f1 ori t a a t c a a g t t t t t t g g g g t c g a g g t g c c g t a a a g c a c t a a a t c g g a a c c c t a a a g g g a g c a t t a g t t c a a a a a a c c c c a g c t c c a c g g c a t t t c g t g a t t t a g c c t t g g g a t t t c c c t c g

4560 f1 ori A G G G A A G A A A F1ori-R c c c c g a t t t a g a g c t t g a c g g g g a a a g c c g g c g a a c g t g g c g a g a a a g g a a g g g a a g a a a g g g g c t a a a t c t c g a a c t g c c c c t t t c g g c c g c t t g c a c c g c t c t t t c c t t c c c t t c t t t

4620 f1 ori G C G A A A G G A G F1ori-R g c g a a a g g a g c g g g c g c t a g g g c g c t g g c a a g t g t a g c g g t c a c g c t g c g c g t a a c c a c c c g c t t t c c t c g c c c g c g a t c c c g c g a c c g t t c a c a t c g c c a g t g c g a c g c g c a t t g g t g g

4680 f1 ori a c a c c c g c c g c g c t t a a t g c g c c g c t a c a g g g c g c g t c g c g g a t c c t g c c t c g c g c g t t t t g t g g g c g g c g c g a a t t a c g c g g c g a t g t c c c g c g c a g c g c c t a g g a c g g a g c g c g c a a a

PfoI 4740 G G A G A C T G T G T A C G T C G A G G G C C pGEX 3' c g g t g a t g a c g g t g a a a a c c t c t g a c a c a t g c a g c t c c c g g a g a c g g t c a c a g c t t g t c t g c c a c t a c t g c c a c t t t t g g a g a c t g t g t a c g t c g a g g g c c t c t g c c a g t g t c g a a c a g a

4800 g t a a g c g g a t g c c g g g a g c a g a c a a g c c c g t c a g g g c g c g t c a g c g g g t g t t g g c g g g t g c a t t c g c c t a c g g c c c t c g t c t g t t c g g g c a g t c c c g c g c a g t c g c c c a c a a c c g c c c a c

AccIBstZ17ITth111IPflFI 4860 bom t c g g g g c g c a g c c a t g a c c c a g t c a c g t a g c g a t a g c g g a g t g t a t a c t g g c t t a a c t a t a g c c c c g c g t c g g t a c t g g g t c a g t g c a t c g c t a t c g c c t c a c a t a t g a c c g a a t t g a t a

4920 bom C G G C A T C A G A G C A G A T T G T A pRS-marker g c g g c a t c a g a g c a g a t t g t a c t g a g a g t g c a c c a t a t g c g g t g t g a a a t a c c g c a c a g a c g c c g t a g t c t c g t c t a a c a t g a c t c t c a c g t g g t a t a c g c c a c a c t t t a t g g c g t g t c t

4980 bom G A G C G A G T G A C T G A G C G A L4440 t g c g t a a g g a g a a a a t a c c g c a t c a g g c g c t c t t c c g c t t c c t c g c t c a c t g a c t c g c t g a c g c a t t c c t c t t t t a t g g c g t a g t c c g c g a g a a g g c g a a g g a g c g a g t g a c t g a g c g a c

5040 c g c t c g g t c g t t c g g c t g c g g c g a g c g g t a t c a g c t c a c t c a a a g g c g g t a a t a c g g t t a g c g a g c c a g c a a g c c g a c g c c g c t c g c c a t a g t c g a g t g a g t t t c c g c c a t t a t g c c a a t

5100 t c c a c a g a a t c a g g g g a t a a c g c a g g a a a g a a c a t g t g a g c a a a a g g c c a g c a a a a g g c c a g g t g t c t t a g t c c c c t a t t g c g t c c t t t c t t g t a c a c t c g t t t t c c g g t c g t t t t c c g g

5160 ori a g g a a c c g t a a a a a g g c c g c g t t g c t g g c g t t t t t c c a t a g g c t c c g c c c c c c t g a c g a g t c c t t g g c a t t t t t c c g g c g c a a c g a c c g c a a a a a g g t a t c c g a g g c g g g g g g a c t g c t c

5220 ori c a t c a c a a a a a t c g a c g c t c a a g t c a g a g g t g g c g a a a c c c g a c a g g a c t a t a a a g a t a c g t a g t g t t t t t a g c t g c g a g t t c a g t c t c c a c c g c t t t g g g c t g t c c t g a t a t t t c t a t g

5280 ori c a g g g t t t c c c c c t g g a a g c t c c c t c g t g c g c t c t c c t g t t c c g a c c c t g c c g c t t a c c g g t c c c a a a g g g g g a c c t t c g a g g g a g c a c g c g a g a g g a c a a g g c t g g g a c g g c g a a t g g c

5340 ori g a t a c c t g t c c g c c t t t c t c c c t t c g g g a a g c g t g g c g c t t t c t c a t a g c t c a c g c t g t a c t a t g g a c a g g c g g a a a g a g g g a a g c c c t t c g c a c c g c g a a a g a g t a t c g a g t g c g a c a t

5400 ori g g t a t c t c a g t t c g g t g t a g g t c g t t c g c t c c a a g c t g g g c t g t g t g c a c g a a c c c c c c g c c a t a g a g t c a a g c c a c a t c c a g c a a g c g a g g t t c g a c c c g a c a c a c g t g c t t g g g g g g c

5460 ori t t c a g c c c g a c c g c t g c g c c t t a t c c g g t a a c t a t c g t c t t g a g t c c a a c c c g g t a a g a c a a g t c g g g c t g g c g a c g c g g a a t a g g c c a t t g a t a g c a g a a c t c a g g t t g g g c c a t t c t g

AlwNI 5520 ori a c g a c t t a t c g c c a c t g g c a g c a g c c a c t g g t a a c a g g a t t a g c a g a g c g a g g t a t g t a g t g c t g a a t a g c g g t g a c c g t c g t c g g t g a c c a t t g t c c t a a t c g t c t c g c t c c a t a c a t c

5580 ori g c g g t g c t a c a g a g t t c t t g a a g t g g t g g c c t a a c t a c g g c t a c a c t a g a a g g a c a g t a t c g c c a c g a t g t c t c a a g a a c t t c a c c a c c g g a t t g a t g c c g a t g t g a t c t t c c t g t c a t a

5640 ori t t g g t a t c t g c g c t c t g c t g a a g c c a g t t a c c t t c g g a a a a a g a g t t g g t a g c t c t t g a t a a c c a t a g a c g c g a g a c g a c t t c g g t c a a t g g a a g c c t t t t t c t c a a c c a t c g a g a a c t a

5700 ori c c g g c a a a c a a a c c a c c g c t g g t a g c g g t g g t t t t t t t g t t t g c a a g c a g c a g a t t a c g c g g c c g t t t g t t t g g t g g c g a c c a t c g c c a c c a a a a a a a c a a a c g t t c g t c g t c t a a t g c g

5760 ori g c a g a a a a a a a g g a t c t c a a g a a g a t c c t t t g a t c t t t t c t a c g g g g t c t g a c g c t c a g t c g t c t t t t t t t c c t a g a g t t c t t c t a g g a a a c t a g a a a a g a t g c c c c a g a c t g c g a g t c a

5820 g g a a c g a a a a c t c a c g t t a a g g g a t t t t g g t c a t g a g a t t a t c a a a a a g g a t c t t c a c c t c c t t g c t t t t g a g t g c a a t t c c c t a a a a c c a g t a c t c t a a t a g t t t t t c c t a g a a g t g g a

5880 a g a t c c t t t t a a a t t a a a a a t g a a g t t t t a a a t c a a t c t a a a g t a t a t a t g a g t a a a c t t t c t a g g a a a a t t t a a t t t t t a c t t c a a a a t t t a g t t a g a t t t c a t a t a t a c t c a t t t g a a

5940 285 280 275 *WHKILSAGIEAIQRNR AmpR *WHKILSAGIEAIQRNR g g t c t g a c a g t t a c c a a t g c t t a a t c a g t g a g g c a c c t a t c t c a g c g a t c t g t c t a t t t c c c a g a c t g t c a a t g g t t a c g a a t t a g t c a c t c c g t g g a t a g a g t c g c t a g a c a g a t a a a g

AhdI 6000 270 265 260 255 EDMTAQSGTTYIVVIRSPKG AmpR EDMTAQSGTTYIVVIRSPKG g t t c a t c c a t a g t t g c c t g a c t c c c c g t c g t g t a g a t a a c t a c g a t a c g g g a g g g c t t a c c a a g t a g g t a t c a a c g g a c t g a g g g g c a g c a c a t c t a t t g a t g c t a t g c c c t c c c g a a t g

BsaI 6060 250 245 240 235 DPGLAAIIGRSGREGAGSKD AmpR DPGLAAIIGRSGREGAGSKD c a t c t g g c c c c a g t g c t g c a a t g a t a c c g c g a g a c c c a c g c t c a c c g g c t c c a g a t t t a t g t a g a c c g g g g t c a c g a c g t t a c t a t g g c g c t c t g g g t g c g a g t g g c c g a g g t c t a a a t a

6120 230 225 220 215 AIFWGAPLASRLLPGAVKDA AmpR AIFWGAPLASRLLPGAVKDA c a g c a a t a a a c c a g c c a g c c g g a a g g g c c g a g c g c a g a a g t g g t c c t g c a a c t t t a t c c g g t c g t t a t t t g g t c g g t c g g c c t t c c c g g c t c g c g t c t t c a c c a g g a c g t t g a a a t a g g c

6180 210 205 200 195 EMWDILQQRSALTLLEGTLL AmpR EMWDILQQRSALTLLEGTLL c c t c c a t c c a g t c t a t t a a t t g t t g c c g g g a a g c t a g a g t a a g t a g t t c g c c a g t t a a t a g g a g g t a g g t c a g a t a a t t a a c a a c g g c c c t t c g a t c t c a t t c a t c a a g c g g t c a a t t a t

PstI 6240 190 185 180 175 KRLTTAMAAPMTTDREDNPI AmpR KRLTTAMAAPMTTDREDNPI g t t t g c g c a a c g t t g t t g c c a t t g c t g c a g g c a t c g t g g t g t c a c g c t c g t c g t t t g g t a c a a a c g c g t t g c a a c a a c g g t a a c g a c g t c c g t a g c a c c a c a g t g c g a g c a g c a a a c c a t

6300 170 165 160 155 AENLEPEWRDLRTVHDGMNH AmpR AENLEPEWRDLRTVHDGMNH t g g c t t c a t t c a g c t c c g g t t c c c a a c g a t c a a g g c g a g t t a c a t g a t c c c c c a t a t t g t a c c g a a g t a a g t c g a g g c c a a g g g t t g c t a g t t c c g c t c a a t g t a c t a g g g g g t a t a a c a

PvuI 6360 150 145 140 135 LFATLEKPGGITTLLLNAAT AmpR LFATLEKPGGITTLLLNAAT g c a a a a a a g c g g t t a g c t c c t t c g g t c c t c c g a t c g t t g t c a g a a g t a a g t t g g c c g c a g c g t t t t t t c g c c a a t c g a g g a a g c c a g g a g g c t a g c a a c a g t c t t c a t t c a a c c g g c g t c

6420 130 125 120 115 NDSMTIAASCLERVTMGDTL AmpR NDSMTIAASCLERVTMGDTL t g t t a t c a c t c a t g g t t a t g g c a g c a c t g c a t a a t t c t c t t a c t g t c a t g c c a t c c g t a a a c a a t a g t g a g t a c c a a t a c c g t c g t g a c g t a t t a a g a g a a t g a c a g t a c g g t a g g c a t t

6480 110 105 100 95 HKETVPSYEVLDNQSYHIRR AmpR HKETVPSYEVLDNQSYHIRR g a t g c t t t t c t g t g a c t g g t g a g t a c t c a a c c a a g t c a t t c t g a g a a t a g t g t a t g c g g c c t a c g a a a a g a c a c t g a c c a c t c a t g a g t t g g t t c a g t a a g a c t c t t a t c a c a t a c g c c g

6540 90 85 80 75 GLQEQGADVRSLVAGCLLVK AmpR A T A A T A C C G C G C C A C A T A G C Amp-R GLQEQGADVRSLVAGCLLVK g a c c g a g t t g c t c t t g c c c g g c g t c a a c a c g g g a t a a t a c c g c g c c a c a t a g c a g a a c t t c t g g c t c a a c g a g a a c g g g c c g c a g t t g t g c c c t a t t a t g g c g c g g t g t a t c g t c t t g a a

6600 70 65 60 55 FTSMMPFREEPRFSELIKGS AmpR FTSMMPFREEPRFSELIKGS t a a a a g t g c t c a t c a t t g g a a a a c g t t c t t c g g g g c g a a a a c t c t c a a g g a t c t t a c c g c a t t t t c a c g a g t a g t a a c c t t t t g c a a g a a g c c c c g c t t t t g a g a g t t c c t a g a a t g g c g

6660 50 45 40 35 NLDLEIYGVRAGLQDEADKV AmpR NLDLEIYGVRAGLQDEADKV t g t t g a g a t c c a g t t c g a t g t a a c c c a c t c g t g c a c c c a a c t g a t c t t c a g c a t c t t t t a a c a a c t c t a g g t c a a g c t a c a t t g g g t g a g c a c g t g g g t t g a c t a g a a g t c g t a g a a a a t

6720 30 25 KVLTEPH 20 15 signal sequence AFVPLCFAAFFPI AmpR KVLTEPHAFVPLCFAAFFPI c t t t c a c c a g c g t t t c t g g g t g a g c a a a a a c a g g a a g g c a a a a t g c c g c a a a a a a g g g a a g a a a g t g g t c g c a a a g a c c c a c t c g t t t t t g t c c t t c c g t t t t a c g g c g t t t t t t c c c t t

6780 10 5 1 signal sequence LAVRFHQISM AmpR AmpR promoter LAVRFHQISM t a a g g g c g a c a c g g a a a t g t t g a a t a c t c a t a c t c t t c c t t t t t c a a t a t t a t t g a a g c a a t t c c c g c t g t g c c t t t a c a a c t t a t g a g t a t g a g a a g g a a a a a g t t a t a a t a a c t t c g t

6840 AmpR promoter t t t a t c a g g g t t a t t g t c t c a t g a g c g g a t a c a t a t t t g a a t g t a t t t a g a a a a a t a a a c a a a t a g t c c c a a t a a c a g a g t a c t c g c c t a t g t a t a a a c t t a c a t a a a t c t t t t t a t t t g

ZraIAatII 6900 AmpR promoter a a a t a g g g g t t c c g c g c a c a t t t c c c c g a a a a g t g c c a c c t g a c g t c t a a g a a a c c a t t a t t t a t c c c c a a g g c g c g t g t a a a g g g g c t t t t c a c g g t g g a c t g c a g a t t c t t t g g t a a t

3ʹ 5ʹ 6912 t t a t c a t g a c a t a a t a g t a c t g t a

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.