Addgene pHH0103_ARHGAP33-1/1 Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: pHH0103_ARHGAP33-1/1 Sequencing Result


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AatII (6903) ZraI (6901) Amp-R (6531 .. 6550) PvuI (6351) PstI (6226) BsaI (6042) AhdI (5981) AlwNI (5504) L4440 (4979 .. 4996) pRS-marker (4879 .. 4898) BstZ17I (4862) AccI (4861) PflFI - Tth111I (4836) pGEX 3' (4716 .. 4738) PfoI (4733) F1ori-R (4568 .. 4587) DraIII (4443) F1ori-F (4356 .. 4377) PsiI (4315) AfeI (3873) BsaBI * (3816) BspEI * (3808) Bpu10I (3725) FspAI (3600) AvaI - BsoBI (3569) pBRforEco (20 .. 38) EcoRI (53) lacIq promoter LacI-R (158 .. 177) MluI (489) BstEII (670) EcoRV (939) HpaI (995) KasI (1128) NarI * (1129) SfoI (1130) PluTI (1132) BfuAI - BspMI (1343) M13/pUC Reverse (1505 .. 1527) lac operator HindIII (1628) EcoNI (1644) SwaI (2061) pGEX 5' (2245 .. 2267) thrombin site Acc65I (2348) KpnI (2352) SfiI (2363) NcoI (2367) PasI (2374) EagI - NotI (2565) BspDI - ClaI (3003) NheI (3346) BmtI (3350) pHH0103_ARHGAP33-1/1 6921 bp

Sequence View

EcoRI 5ʹ 3ʹ 60 A A T A G G C G T A T C A C G A G G C pBRforEco c a t g a c a t t a a c c t a t a a a a a t a g g c g t a t c a c g a g g c c c t t t c g t c t t c a a g a a t t c c c g t a c t g t a a t t g g a t a t t t t t a t c c g c a t a g t g c t c c g g g a a a g c a g a a g t t c t t a a g g g

120 lacIq promoter g a c a c c a t c g a a t g g t g c a a a a c c t t t c g c g g t a t g g c a t g a t a g c g c c c g g a a g a g a g t c t g t g g t a g c t t a c c a c g t t t t g g a a a g c g c c a t a c c g t a c t a t c g c g g g c c t t c t c t c a

180 1 5 10 MKPVTLYDVAEYAG lacIq promoter lacI T G C T A C A G C G T C T C A T A C G G LacI-R c a a t t c a g g g t g g t g a a t g t g a a a c c a g t a a c g t t a t a c g a t g t c g c a g a g t a t g c c g g t g t t a a g t c c c a c c a c t t a c a c t t t g g t c a t t g c a a t a t g c t a c a g c g t c t c a t a c g g c c a

240 15 20 25 30 VSYQTVSRVVNQASHVSAKT lacI g t c t c t t a t c a g a c c g t t t c c c g c g t g g t g a a c c a g g c c a g c c a c g t t t c t g c g a a a a c g c a g a g a a t a g t c t g g c a a a g g g c g c a c c a c t t g g t c c g g t c g g t g c a a a g a c g c t t t t g c

300 35 40 45 50 REKVEAAMAELNYIPNRVAQ lacI MAELNYIPNRVAQ c g g g a a a a a g t g g a a g c g g c g a t g g c g g a g c t g a a t t a c a t t c c c a a c c g c g t g g c a c a a g c c c t t t t t c a c c t t c g c c g c t a c c g c c t c g a c t t a a t g t a a g g g t t g g c g c a c c g t g t t

360 55 60 65 70 QLAGKQSLLIGVATSSLALH lacI QLAGKQSLLIGVATSSLALH c a a c t g g c g g g c a a a c a g t c g t t g c t g a t t g g c g t t g c c a c c t c c a g t c t g g c c c t g c a c g t t g a c c g c c c g t t t g t c a g c a a c g a c t a a c c g c a a c g g t g g a g g t c a g a c c g g g a c g t g

420 75 80 85 90 APSQIVAAIKSRADQLGASV lacI APSQIVAAIKSRADQLGASV g c g c c g t c g c a a a t t g t c g c g g c g a t t a a a t c t c g c g c c g a t c a a c t g g g t g c c a g c g t g c g c g g c a g c g t t t a a c a g c g c c g c t a a t t t a g a g c g c g g c t a g t t g a c c c a c g g t c g c a c

480 95 100 105 110 VVSMVERSGVEACKAAVHNL lacI VVSMVERSGVEACKAAVHNL g t g g t g t c g a t g g t a g a a c g a a g c g g c g t c g a a g c c t g t a a a g c g g c g g t g c a c a a t c t t c a c c a c a g c t a c c a t c t t g c t t c g c c g c a g c t t c g g a c a t t t c g c c g c c a c g t g t t a g a a

MluI 540 115 120 125 130 LAQRVSGLIINYPLDDQDAI lacI LAQRVSGLIINYPLDDQDAI c t c g c g c a a c g c g t c a g t g g g c t g a t c a t t a a c t a t c c g c t g g a t g a c c a g g a t g c c a t t g a g c g c g t t g c g c a g t c a c c c g a c t a g t a a t t g a t a g g c g a c c t a c t g g t c c t a c g g t a a

600 135 140 145 150 AVEAACTNVPALFLDVSDQT lacI AVEAACTNVPALFLDVSDQT g c t g t g g a a g c t g c c t g c a c t a a t g t t c c g g c g t t a t t t c t t g a t g t c t c t g a c c a g a c a c g a c a c c t t c g a c g g a c g t g a t t a c a a g g c c g c a a t a a a g a a c t a c a g a g a c t g g t c t g t

660 155 160 165 170 PINSIIFSHEDGTRLGVEHL lacI PINSIIFSHEDGTRLGVEHL c c c a t c a a c a g t a t t a t t t t c t c c c a t g a a g a c g g t a c g c g a c t g g g c g t g g a g c a t c t g g g g t a g t t g t c a t a a t a a a a g a g g g t a c t t c t g c c a t g c g c t g a c c c g c a c c t c g t a g a c

BstEII 720 175 180 185 190 VALGHQQIALLAGPLSSVSA lacI VALGHQQIALLAGPLSSVSA g t c g c a t t g g g t c a c c a g c a a a t c g c g c t g t t a g c g g g c c c a t t a a g t t c t g t c t c g g c g c a g c g t a a c c c a g t g g t c g t t t a g c g c g a c a a t c g c c c g g g t a a t t c a a g a c a g a g c c g c

780 195 200 205 210 RLRLAGWHKYLTRNQIQPIA lacI RLRLAGWHKYLTRNQIQPIA c g t c t g c g t c t g g c t g g c t g g c a t a a a t a t c t c a c t c g c a a t c a a a t t c a g c c g a t a g c g g c a g a c g c a g a c c g a c c g a c c g t a t t t a t a g a g t g a g c g t t a g t t t a a g t c g g c t a t c g c

840 215 220 225 230 EREGDWSAMSGFQQTMQMLN lacI EREGDWSAMSGFQQTMQMLN g a a c g g g a a g g c g a c t g g a g t g c c a t g t c c g g t t t t c a a c a a a c c a t g c a a a t g c t g a a t c t t g c c c t t c c g c t g a c c t c a c g g t a c a g g c c a a a a g t t g t t t g g t a c g t t t a c g a c t t a

900 235 240 245 250 EGIVPTAMLVANDQMALGAM lacI EGIVPTAMLVANDQMALGAM g a g g g c a t c g t t c c c a c t g c g a t g c t g g t t g c c a a c g a t c a g a t g g c g c t g g g c g c a a t g c t c c c g t a g c a a g g g t g a c g c t a c g a c c a a c g g t t g c t a g t c t a c c g c g a c c c g c g t t a c

EcoRV 960 255 260 265 270 RAITESGLRVGADISVVGYD lacI RAITESGLRVGADISVVGYD c g c g c c a t t a c c g a g t c c g g g c t g c g c g t t g g t g c g g a t a t c t c g g t a g t g g g a t a c g a c g c g c g g t a a t g g c t c a g g c c c g a c g c g c a a c c a c g c c t a t a g a g c c a t c a c c c t a t g c t g

HpaI 1020 275 280 285 290 DTEDSSCYIPPLTTIKQDFR lacI DTEDSSCYIPPLTTIKQDFR g a t a c c g a a g a c a g c t c a t g t t a t a t c c c g c c g t t a a c c a c c a t c a a a c a g g a t t t t c g c c t a t g g c t t c t g t c g a g t a c a a t a t a g g g c g g c a a t t g g t g g t a g t t t g t c c t a a a a g c g

1080 295 300 305 310 LLGQTSVDRLLQLSQGQAVK lacI LLGQTSVDRLLQLSQGQAVK c t g c t g g g g c a a a c c a g c g t g g a c c g c t t g c t g c a a c t c t c t c a g g g c c a g g c g g t g a a g g a c g a c c c c g t t t g g t c g c a c c t g g c g a a c g a c g t t g a g a g a g t c c c g g t c c g c c a c t t c

KasINarI*SfoIPluTI 1140 315 320 325 330 GNQLLPVSLVKRKTTLAPNT lacI GNQLLPVSLVKRKTTLAPNT g g c a a t c a g c t g t t g c c c g t c t c a c t g g t g a a a a g a a a a a c c a c c c t g g c g c c c a a t a c g c c g t t a g t c g a c a a c g g g c a g a g t g a c c a c t t t t c t t t t t g g t g g g a c c g c g g g t t a t g c

1200 335 340 345 350 QTASPRALADSLMQLARQVS lacI QTASPRALADSLMQLARQVS c a a a c c g c c t c t c c c c g c g c g t t g g c c g a t t c a t t a a t g c a g c t g g c a c g a c a g g t t t c c g t t t g g c g g a g a g g g g c g c g c a a c c g g c t a a g t a a t t a c g t c g a c c g t g c t g t c c a a a g g

1260 355 360 RLESGQ* lacI RLESGQ* c g a c t g g a a a g c g g g c a g t g a g c g c a a c g c a a t t a a t g t a a g t t a g c t c a c t c a t t a g g c g c t g a c c t t t c g c c c g t c a c t c g c g t t g c g t t a a t t a c a t t c a a t c g a g t g a g t a a t c c g

1320 a c a a t t c t c a t g t t t g a c a g c t t a t c a t c g a c t g c a c g g t g c a c c a a t g c t t c t g g c g t c t g t t a a g a g t a c a a a c t g t c g a a t a g t a g c t g a c g t g c c a c g t g g t t a c g a a g a c c g c a g

BspMIBfuAI 1380 a g g c a g c c a t c g g a a g c t g t g g t a t g g c t g t g c a g g t c g t a a a t c a c t g c a t a a t t c g t g t c c g t c g g t a g c c t t c g a c a c c a t a c c g a c a c g t c c a g c a t t t a g t g a c g t a t t a a g c a c

1440 t c g c t c a a g g c g c a c t c c c g t t c t g g a t a a t g t t t t t t g c g c c g a c a t c a t a a c g g t t c t a g c g a g t t c c g c g t g a g g g c a a g a c c t a t t a c a a a a a a c g c g g c t g t a g t a t t g c c a a g a

1500 -35 -10 tac promoter lac operator g g c a a a t a t t c t g a a a t g a g c t g t t g a c a a t t a a t c a t c g g c t c g t a t a a t g t g t g g a a t c c g t t t a t a a g a c t t t a c t c g a c a a c t g t t a a t t a g t a g c c g a g c a t a t t a c a c a c c t t a

1560 lac operator A G C G G A T A A C A A T T T C A C A C A G G M13/pUC Reverse t g t g a g c g g a t a a c a a t t t c a c a c a g g a a a c a g c c a g t c c g t t t a g g t g t t t t c a c g a g c a c a c t c g c c t a t t g t t a a a g t g t g t c c t t t g t c g g t c a g g c a a a t c c a c a a a a g t g c t c g

1620 1 5 HHHHHH S 6xHis MKIEEHHHHHHS a c t t c a c c a a c a a g g a c c a t a g a t t a t g a a a a t c g a a g a a c a c c a t c a c c a t c a c c a t t c t g a a g t g g t t g t t c c t g g t a t c t a a t a c t t t t a g c t t c t t g t g g t a g t g g t a g t g g t a a g

EcoNIHindIII 1680 (in frame with 6xHis) SGKL 1 5 10 15 MSPILGYWKIKGLVQP GST SGKLMSPILGYWKIKGLVQP c a g c g g t a a g c t t a t g t c c c c t a t a c t a g g t t a t t g g a a a a t t a a g g g c c t t g t g c a a c c g t c g c c a t t c g a a t a c a g g g g a t a t g a t c c a a t a a c c t t t t a a t t c c c g g a a c a c g t t g g

1740 20 25 30 35 TRLLLEYLEEKYEEHLYERD GST TRLLLEYLEEKYEEHLYERD c a c t c g a c t t c t t t t g g a a t a t c t t g a a g a a a a a t a t g a a g a g c a t t t g t a t g a g c g c g a g t g a g c t g a a g a a a a c c t t a t a g a a c t t c t t t t t a t a c t t c t c g t a a a c a t a c t c g c g c t

1800 40 45 50 55 EGDKWRNKKFELGLEFPNLP GST EGDKWRNKKFELGLEFPNLP t g a a g g t g a t a a a t g g c g a a a c a a a a a g t t t g a a t t g g g t t t g g a g t t t c c c a a t c t t c c a c t t c c a c t a t t t a c c g c t t t g t t t t t c a a a c t t a a c c c a a a c c t c a a a g g g t t a g a a g g

1860 60 65 70 75 YYIDGDVKLTQSMAIIRYIA GST YYIDGDVKLTQSMAIIRYIA t t a t t a t a t t g a t g g t g a t g t t a a a t t a a c a c a g t c t a t g g c c a t c a t a c g t t a t a t a g c a a t a a t a t a a c t a c c a c t a c a a t t t a a t t g t g t c a g a t a c c g g t a g t a t g c a a t a t a t c g

1920 80 85 90 95 DKHNMLGGCPKERAEISMLE GST DKHNMLGGCPKERAEISMLE t g a c a a g c a c a a c a t g t t g g g t g g t t g t c c a a a a g a g c g t g c a g a g a t t t c a a t g c t t g a a c t g t t c g t g t t g t a c a a c c c a c c a a c a g g t t t t c t c g c a c g t c t c t a a a g t t a c g a a c t

1980 100 105 110 115 GAVLDIRYGVSRIAYSKDFE GST GAVLDIRYGVSRIAYSKDFE a g g a g c g g t t t t g g a t a t t a g a t a c g g t g t t t c g a g a a t t g c a t a t a g t a a a g a c t t t g a t c c t c g c c a a a a c c t a t a a t c t a t g c c a c a a a g c t c t t a a c g t a t a t c a t t t c t g a a a c t

2040 120 125 130 135 TLKVDFLSKLPEMLKMFEDR GST TLKVDFLSKLPEMLKMFEDR a a c t c t c a a a g t t g a t t t t c t t a g c a a g c t a c c t g a a a t g c t g a a a a t g t t c g a a g a t c g t t g a g a g t t t c a a c t a a a a g a a t c g t t c g a t g g a c t t t a c g a c t t t t a c a a g c t t c t a g c

SwaI 2100 140 145 150 155 LCHKTYLNGDHVTHPDFMLY GST LCHKTYLNGDHVTHPDFMLY t t t a t g t c a t a a a a c a t a t t t a a a t g g t g a t c a t g t a a c c c a t c c t g a c t t c a t g t t g t a a a a t a c a g t a t t t t g t a t a a a t t t a c c a c t a g t a c a t t g g g t a g g a c t g a a g t a c a a c a t

2160 160 165 170 175 DALDVVLYMDPMCLDAFPKL GST DALDVVLYMDPMCLDAFPKL t g a c g c t c t t g a t g t t g t t t t a t a c a t g g a c c c a a t g t g c c t g g a t g c g t t c c c a a a a t t a c t g c g a g a a c t a c a a c a a a a t a t g t a c c t g g g t t a c a c g g a c c t a c g c a a g g g t t t t a a

2220 180 185 190 195 VCFKKRIEAIPQIDKYLKSS GST VCFKKRIEAIPQIDKYLKSS a g t t t g t t t t a a a a a a c g t a t t g a a g c t a t c c c a c a a a t t g a t a a g t a c t t g a a a t c c a g t c a a a c a a a a t t t t t t g c a t a a c t t c g a t a g g g t g t t t a a c t a t t c a t g a a c t t t a g g t c

2280 200 205 210 215 KYIAWPLQGWQATFGGGDHP GST G G G C T G G C A A G C C A C G T T T G G T G pGEX 5' KYIAWPLQGWQATFGGGDHP c a a g t a t a t a g c a t g g c c t t t g c a g g g c t g g c a a g c c a c g t t t g g t g g t g g c g a c c a t c c g t t c a t a t a t c g t a c c g g a a a c g t c c c g a c c g t t c g g t g c a a a c c a c c a c c g c t g g t a g g

2340 PK SD 1 5 LEVLFQGP LSSG 1 LVPR GST HRV 3C site thrombin site PKSDLEVLFQGPLSSGLVPR t c c a a a a t c g g a t c t g g a a g t t c t g t t c c a g g g g c c c c t g t c c a g c g g t c t g g t t c c g c g a g g t t t t a g c c t a g a c c t t c a a g a c a a g g t c c c c g g g g a c a g g t c g c c a g a c c a a g g c g c

PasINcoISfiIAcc65IKpnI 2400 5 GS (in frame with thrombin site) GTAAQPAMAQGALAAAHV thrombin site GSGTAAQPAMAQGALAAAHV M t g g t t c c g g t a c c g c g g c c c a g c c g g c c a t g g c c c a g g g c g c g c t g g c a g c a g c c c a t g t a c c a a g g c c a t g g c g c c g g g t c g g c c g g t a c c g g g t c c c g c g c g a c c g t c g t c g g g t a c a

2460 (in frame with thrombin site) IKRYTAQAPDELSFEVGDIV IKRYTAQAPDELSFEVGDIV LSNVIPPKHQMNCPSKLVIL t a t c a a a c g t t a t a c c g c c c a a g c a c c a g a t g a a c t g t c c t t c g a a g t t g g t g a t a t t g t a t a g t t t g c a a t a t g g c g g g t t c g t g g t c t a c t t g a c a g g a a g c t t c a a c c a c t a t a a c a

2520 (in frame with thrombin site) SVIDMPPTEDRSWWRGKRGF SVIDMPPTEDRSWWRGKRGF SPLLICHRPKIVLGGVVNVV c t c c g t t a t t g a t a t g c c a c c g a c c g a a g a t c g t t c t t g g t g g c g t g g t a a a c g t g g t t t g a g g c a a t a a c t a t a c g g t g g c t g g c t t c t a g c a a g a a c c a c c g c a c c a t t t g c a c c a a a

EagINotI 2580 (in frame with thrombin site) QVGFFPSECVELFTAAA* fd terminator QVGFFPSECVELFTAAA* FKSVSSRPNVLNCLPRPHNK t c a a g t c g g t t t c t t c c c g t c c g a a t g t g t t g a a c t g t t t a c c g c g g c c g c a t a a t a a a c a g t t c a g c c a a a g a a g g g c a g g c t t a c a c a a c t t g a c a a a t g g c g c c g g c g t a t t a t t t g

2640 fd terminator PIQLKAPFGAFFLEIFNVKK c g a t a c a a t t a a a g g c t c c t t t t g g a g c c t t t t t t t t g g a g a t t t t c a a c g t g a a a a a a t g c t a t g t t a a t t t c c g a g g a a a a c c t c g g a a a a a a a a c c t c t a a a a g t t g c a c t t t t t t a

2700 LLFAIPLVVPFYSHSAETVE t a t t a t t c g c a a t t c c t t t a g t t g t t c c t t t c t a t t c t c a c t c c g c t g a a a c t g t t g a a a a t a a t a a g c g t t a a g g a a a t c a a c a a g g a a a g a t a a g a g t g a g g c g a c t t t g a c a a c t t t

2760 SCLAKPHTENSFTNVWKDDK g t t g t t t a g c a a a a c c c c a t a c a g a a a a t t c a t t t a c t a a c g t c t g g a a a g a c g a c a a a a c a a c a a a t c g t t t t g g g g t a t g t c t t t t a a g t a a a t g a t t g c a g a c c t t t c t g c t g t t t t

2820 TLDRYANYEGCLWNATGVVV c t t t a g a t c g t t a c g c t a a c t a t g a g g g c t g t c t g t g g a a t g c t a c a g g c g t t g t a g t t t g a a a t c t a g c a a t g c g a t t g a t a c t c c c g a c a g a c a c c t t a c g a t g t c c g c a a c a t c a a a

2880 CTGDETQCLARVAVALVPVI g t a c t g g t g a c g a a a c t c a g t g t c t a g c t a g a g t g g c g g t g g c t c t g g t t c c g g t g a t t t c a t g a c c a c t g c t t t g a g t c a c a g a t c g a t c t c a c c g c c a c c g a g a c c a a g g c c a c t a a a

2940 LIMKRWQTLIRGL* t g a t t a t g a a a a g a t g g c a a a c g c t a a t a a g g g g g c t a t g a c c g a a a a t g c c g a t g a a a a a c t a a t a c t t t t c t a c c g t t t g c g a t t a t t c c c c c g a t a c t g g c t t t t a c g g c t a c t t t t

3000 c g c g c t a c a g t c t g a c g c t a a a g g c a a a c t t g a t t c t g t c g c t a c t g a t t a c g g t g c t g c g c g c g a t g t c a g a c t g c g a t t t c c g t t t g a a c t a a g a c a g c g a t g a c t a a t g c c a c g a c g

BspDIClaI 3060 t a t c g a t g g t t t c a t t g g t g a c g t t t c c g g c c t t g c t a a t g g t a a t g g t g c t a c t g g t g a a t a g c t a c c a a a g t a a c c a c t g c a a a g g c c g g a a c g a t t a c c a t t a c c a c g a t g a c c a c t

3120 t t t t g c t g g c t c t a a t t c c c a a a t g g c t c a a g t c g g t g a c g g t g a t a a t t c a c c t t t a a t a a a a c g a c c g a g a t t a a g g g t t t a c c g a g t t c a g c c a c t g c c a c t a t t a a g t g g a a a t t a

3180 g a a t a a t t t c c g t c a a t a t t t a c c t t c c c t c c c t c a a t c g g t t g a a t g t c g c c c t t t t g t c t t a t t a a a g g c a g t t a t a a a t g g a a g g g a g g g a g t t a g c c a a c t t a c a g c g g g a a a a c a

3240 c t t t g g c g c t g g t a a a c c a t a t g a a t t t t c t a t t g a t t g t g a c a a a a t a a a c t t a t t c c g g a a a c c g c g a c c a t t t g g t a t a c t t a a a a g a t a a c t a a c a c t g t t t t a t t t g a a t a a g g c

3300 t g g t g t c t t t g c g t t t c t t t t a t a t g t t g c c a c c t t t a t g t a t g t a t t t t c t a c g t t t g c a c c a c a g a a a c g c a a a g a a a a t a t a c a a c g g t g g a a a t a c a t a c a t a a a a g a t g c a a a c g

BmtINheI 3360 t a a c a t a c t g c g t a a t a a g g a g t c t t a a t c a t g c c a g t t c t t t t g g c t a g c g c c g c c c t a a t t g t a t g a c g c a t t a t t c c t c a g a a t t a g t a c g g t c a a g a a a a c c g a t c g c g g c g g g a t

3420 t a c c t t g t c t g c c t c c c c g c g t t g c g t c g c g g t g c a t g g a g c c g g g c c a c c t c g a c c t g a a t g g a a c a g a c g g a g g g g c g c a a c g c a g c g c c a c g t a c c t c g g c c c g g t g g a g c t g g a c t

3480 a t g g a a g c c g g c g g c a c c t c g c t a a c g g a t t c a c c a c t c c a a g a a t t g g a g c c a a t c a a t t a c c t t c g g c c g c c g t g g a g c g a t t g c c t a a g t g g t g a g g t t c t t a a c c t c g g t t a g t t a

3540 t c t t g c g g a g a a c t g t g a a t g c g c a a a c c a a c c c t t g g c a g a a c a t a t c c a t c g c g t c c g a g a a c g c c t c t t g a c a c t t a c g c g t t t g g t t g g g a a c c g t c t t g t a t a g g t a g c g c a g g c

FspAIBsoBIAvaI 3600 c c a t c t c c a g c a g c c g c a c g c g g c g c a t c t c g g g c a g c g t t g g g t c c t g g c c a c g g g t g c g g t a g a g g t c g t c g g c g t g c g c c g c g t a g a g c c c g t c g c a a c c c a g g a c c g g t g c c c a c g

3660 g c a t g a t c g t g c t c c t g t c g t t g a g g a c c c g g c t a g g c t g g c g g g g t t g c c t t a c t g g t t c g t a c t a g c a c g a g g a c a g c a a c t c c t g g g c c g a t c c g a c c g c c c c a a c g g a a t g a c c a a

3720 a g c a g a a t g a a t c a c c g a t a c g c g a g c g a a c g t g a a g c g a c t g c t g c t g c a a a a c g t c t g t c g t c t t a c t t a g t g g c t a t g c g c t c g c t t g c a c t t c g c t g a c g a c g a c g t t t t g c a g a c

Bpu10I 3780 c g a c c t g a g c a a c a a c a t g a a t g g t c t t c g g t t t c c g t g t t t c g t a a a g t c t g g a a a c g c g c t g g a c t c g t t g t t g t a c t t a c c a g a a g c c a a a g g c a c a a a g c a t t t c a g a c c t t t g c g

BsaBI*BspEI* 3840 g g a a g t c a g c g c c c t g c a c c a t t a t g t t c c g g a t c t g c a t c g c a g g a t g c t g c t g g c t a c c c t t c a g t c g c g g g a c g t g g t a a t a c a a g g c c t a g a c g t a g c g t c c t a c g a c g a c c g a t g

AfeI 3900 c c t g t g g a a c a c c t a c a t c t g t a t t a a c g a a g c g c t g g c a t t g a c c c t g a g t g a t t t t t c g g a c a c c t t g t g g a t g t a g a c a t a a t t g c t t c g c g a c c g t a a c t g g g a c t c a c t a a a a a g

3960 t c t g g t c c c g c c g c a t c c a t a c c g c c a g t t g t t t a c c c t c a c a a c g t t c c a g t a a c c g g g a g a c c a g g g c g g c g t a g g t a t g g c g g t c a a c a a a t g g g a g t g t t g c a a g g t c a t t g g c c c

4020 c a t g t t c a t c a t c a g t a a c c c g t a t c g t g a g c a t c c t c t c t c g t t t c a t c g g t a t c a t t a g t a c a a g t a g t a g t c a t t g g g c a t a g c a c t c g t a g g a g a g a g c a a a g t a g c c a t a g t a a t

4080 c c c c c a t g a a c a g a a a t c c c c c t t a c a c g g a g g c a t c a g t g a c c a a a c a g g a a a a a a c c g g g g g g t a c t t g t c t t t a g g g g g a a t g t g c c t c c g t a g t c a c t g g t t t g t c c t t t t t t g g c

4140 c c c t t a a c a t g g c c c g c t t t a t c a g a a g c c a g a c a t t a a c g c t t c t g g a g a a a c t c a a c g g g g a a t t g t a c c g g g c g a a a t a g t c t t c g g t c t g t a a t t g c g a a g a c c t c t t t g a g t t g c

4200 a g c t g g a c g c g g a t g a a c a g g c a g a c a t c t g t g a a t c g c t t c a c g a c c a c g c t g a t g a g c t c g a c c t g c g c c t a c t t g t c c g t c t g t a g a c a c t t a g c g a a g t g c t g g t g c g a c t a c t c g

4260 f1 ori t t t a c c g c a g g a t c c g c g a a a t t g t a a g c g t t a a t a t t t t g t t a a a a t t c g c g t t a a a t t a a a t g g c g t c c t a g g c g c t t t a a c a t t c g c a a t t a t a a a a c a a t t t t a a g c g c a a t t t a a

PsiI 4320 f1 ori t t t g t t a a a t c a g c t c a t t t t t t a a c c a a t a g g c c g a a a t c g g c a a a a t c c c t t a t a a a t a a a c a a t t t a g t c g a g t a a a a a a t t g g t t a t c c g g c t t t a g c c g t t t t a g g g a a t a t t t a

4380 f1 ori G G T C A A A C C T T G T T C T C A G G T G F1ori-F c a a a a g a a t a g a c c g a g a t a g g g t t g a g t g t t g t t c c a g t t t g g a a c a a g a g t c c a c t a t g t t t t c t t a t c t g g c t c t a t c c c a a c t c a c a a c a a g g t c a a a c c t t g t t c t c a g g t g a t a

4440 f1 ori t a a a g a a c g t g g a c t c c a a c g t c a a a g g g c g a a a a a c c g t c t a t c a g g g c g a t g g c c c a c a t t t c t t g c a c c t g a g g t t g c a g t t t c c c g c t t t t t g g c a g a t a g t c c c g c t a c c g g g t g

DraIII 4500 f1 ori t a c g t g a a c c a t c a c c c t a a t c a a g t t t t t t g g g g t c g a g g t g c c g t a a a g c a c t a a a t c a t g c a c t t g g t a g t g g g a t t a g t t c a a a a a a c c c c a g c t c c a c g g c a t t t c g t g a t t t a g

4560 f1 ori g g a a c c c t a a a g g g a g c c c c c g a t t t a g a g c t t g a c g g g g a a a g c c g g c g a a c g t g g c g a c c t t g g g a t t t c c c t c g g g g g c t a a a t c t c g a a c t g c c c c t t t c g g c c g c t t g c a c c g c t

4620 f1 ori A G G G A A G A A A G C G A A A G G A G F1ori-R g a a a g g a a g g g a a g a a a g c g a a a g g a g c g g g c g c t a g g g c g c t g g c a a g t g t a g c g g t c a c t t t c c t t c c c t t c t t t c g c t t t c c t c g c c c g c g a t c c c g c g a c c g t t c a c a t c g c c a g t

4680 f1 ori c g c t g c g c g t a a c c a c c a c a c c c g c c g c g c t t a a t g c g c c g c t a c a g g g c g c g t c g c g g a g c g a c g c g c a t t g g t g g t g t g g g c g g c g c g a a t t a c g c g g c g a t g t c c c g c g c a g c g c c t

PfoI 4740 G G A G A C T G T G T A C G T C G A G G G C C pGEX 3' t c c t g c c t c g c g c g t t t c g g t g a t g a c g g t g a a a a c c t c t g a c a c a t g c a g c t c c c g g a g a g g a c g g a g c g c g c a a a g c c a c t a c t g c c a c t t t t g g a g a c t g t g t a c g t c g a g g g c c t c

4800 a c g g t c a c a g c t t g t c t g t a a g c g g a t g c c g g g a g c a g a c a a g c c c g t c a g g g c g c g t c a t g c c a g t g t c g a a c a g a c a t t c g c c t a c g g c c c t c g t c t g t t c g g g c a g t c c c g c g c a g t

Tth111IPflFI 4860 bom g c g g g t g t t g g c g g g t g t c g g g g c g c a g c c a t g a c c c a g t c a c g t a g c g a t a g c g g a g t g c g c c c a c a a c c g c c c a c a g c c c c g c g t c g g t a c t g g g t c a g t g c a t c g c t a t c g c c t c a c

AccIBstZ17I 4920 bom C G G C A T C A G A G C A G A T T G T A pRS-marker t a t a c t g g c t t a a c t a t g c g g c a t c a g a g c a g a t t g t a c t g a g a g t g c a c c a t a t g c g g t a t a t g a c c g a a t t g a t a c g c c g t a g t c t c g t c t a a c a t g a c t c t c a c g t g g t a t a c g c c a

4980 bom G A L4440 g t g a a a t a c c g c a c a g a t g c g t a a g g a g a a a a t a c c g c a t c a g g c g c t c t t c c g c t t c c t c a c t t t a t g g c g t g t c t a c g c a t t c c t c t t t t a t g g c g t a g t c c g c g a g a a g g c g a a g g a

5040 G C G A G T G A C T G A G C G A L4440 c g c t c a c t g a c t c g c t g c g c t c g g t c g t t c g g c t g c g g c g a g c g g t a t c a g c t c a c t c a a g c g a g t g a c t g a g c g a c g c g a g c c a g c a a g c c g a c g c c g c t c g c c a t a g t c g a g t g a g t t

5100 a g g c g g t a a t a c g g t t a t c c a c a g a a t c a g g g g a t a a c g c a g g a a a g a a c a t g t g a g c a a t c c g c c a t t a t g c c a a t a g g t g t c t t a g t c c c c t a t t g c g t c c t t t c t t g t a c a c t c g t t

5160 ori a a g g c c a g c a a a a g g c c a g g a a c c g t a a a a a g g c c g c g t t g c t g g c g t t t t t c c a t a g g c t t c c g g t c g t t t t c c g g t c c t t g g c a t t t t t c c g g c g c a a c g a c c g c a a a a a g g t a t c c g

5220 ori t c c g c c c c c c t g a c g a g c a t c a c a a a a a t c g a c g c t c a a g t c a g a g g t g g c g a a a c c c g a a g g c g g g g g g a c t g c t c g t a g t g t t t t t a g c t g c g a g t t c a g t c t c c a c c g c t t t g g g c t

5280 ori c a g g a c t a t a a a g a t a c c a g g g t t t c c c c c t g g a a g c t c c c t c g t g c g c t c t c c t g t t c c g t c c t g a t a t t t c t a t g g t c c c a a a g g g g g a c c t t c g a g g g a g c a c g c g a g a g g a c a a g g

5340 ori g a c c c t g c c g c t t a c c g g a t a c c t g t c c g c c t t t c t c c c t t c g g g a a g c g t g g c g c t t t c c t g g g a c g g c g a a t g g c c t a t g g a c a g g c g g a a a g a g g g a a g c c c t t c g c a c c g c g a a a g

5400 ori t c a t a g c t c a c g c t g t a g g t a t c t c a g t t c g g t g t a g g t c g t t c g c t c c a a g c t g g g c t g a g t a t c g a g t g c g a c a t c c a t a g a g t c a a g c c a c a t c c a g c a a g c g a g g t t c g a c c c g a c

5460 ori t g t g c a c g a a c c c c c c g t t c a g c c c g a c c g c t g c g c c t t a t c c g g t a a c t a t c g t c t t g a a c a c g t g c t t g g g g g g c a a g t c g g g c t g g c g a c g c g g a a t a g g c c a t t g a t a g c a g a a c t

AlwNI 5520 ori g t c c a a c c c g g t a a g a c a c g a c t t a t c g c c a c t g g c a g c a g c c a c t g g t a a c a g g a t t a g c a g g t t g g g c c a t t c t g t g c t g a a t a g c g g t g a c c g t c g t c g g t g a c c a t t g t c c t a a t c

5580 ori c a g a g c g a g g t a t g t a g g c g g t g c t a c a g a g t t c t t g a a g t g g t g g c c t a a c t a c g g c t a g t c t c g c t c c a t a c a t c c g c c a c g a t g t c t c a a g a a c t t c a c c a c c g g a t t g a t g c c g a t

5640 ori c a c t a g a a g g a c a g t a t t t g g t a t c t g c g c t c t g c t g a a g c c a g t t a c c t t c g g a a a a a g g t g a t c t t c c t g t c a t a a a c c a t a g a c g c g a g a c g a c t t c g g t c a a t g g a a g c c t t t t t c

5700 ori a g t t g g t a g c t c t t g a t c c g g c a a a c a a a c c a c c g c t g g t a g c g g t g g t t t t t t t g t t t g t c a a c c a t c g a g a a c t a g g c c g t t t g t t t g g t g g c g a c c a t c g c c a c c a a a a a a a c a a a c

5760 ori c a a g c a g c a g a t t a c g c g c a g a a a a a a a g g a t c t c a a g a a g a t c c t t t g a t c t t t t c t a c g t t c g t c g t c t a a t g c g c g t c t t t t t t t c c t a g a g t t c t t c t a g g a a a c t a g a a a a g a t g

5820 g g g g t c t g a c g c t c a g t g g a a c g a a a a c t c a c g t t a a g g g a t t t t g g t c a t g a g a t t a t c c c c c a g a c t g c g a g t c a c c t t g c t t t t g a g t g c a a t t c c c t a a a a c c a g t a c t c t a a t a g

5880 a a a a a g g a t c t t c a c c t a g a t c c t t t t a a a t t a a a a a t g a a g t t t t a a a t c a a t c t a a a g t t t t t c c t a g a a g t g g a t c t a g g a a a a t t t a a t t t t t a c t t c a a a a t t t a g t t a g a t t t c

5940 285 280 *WHKILSAGIE AmpR *WHKILSAGIE t a t a t a t g a g t a a a c t t g g t c t g a c a g t t a c c a a t g c t t a a t c a g t g a g g c a c c t a t c t c a t a t a t a c t c a t t t g a a c c a g a c t g t c a a t g g t t a c g a a t t a g t c a c t c c g t g g a t a g a g

AhdI 6000 275 270 265 260 AIQRNREDMTAQSGTTYIVV AmpR AIQRNREDMTAQSGTTYIVV a g c g a t c t g t c t a t t t c g t t c a t c c a t a g t t g c c t g a c t c c c c g t c g t g t a g a t a a c t a c t c g c t a g a c a g a t a a a g c a a g t a g g t a t c a a c g g a c t g a g g g g c a g c a c a t c t a t t g a t g

BsaI 6060 255 250 245 240 IRSPKGDPGLAAIIGRSGRE AmpR IRSPKGDPGLAAIIGRSGRE g a t a c g g g a g g g c t t a c c a t c t g g c c c c a g t g c t g c a a t g a t a c c g c g a g a c c c a c g c t c c t a t g c c c t c c c g a a t g g t a g a c c g g g g t c a c g a c g t t a c t a t g g c g c t c t g g g t g c g a g

6120 235 230 225 220 GAGSKDAIFWGAPLASRLLP AmpR GAGSKDAIFWGAPLASRLLP a c c g g c t c c a g a t t t a t c a g c a a t a a a c c a g c c a g c c g g a a g g g c c g a g c g c a g a a g t g g t g g c c g a g g t c t a a a t a g t c g t t a t t t g g t c g g t c g g c c t t c c c g g c t c g c g t c t t c a c c

6180 215 210 205 200 GAVKDAEMWDILQQRSALTL AmpR GAVKDAEMWDILQQRSALTL t c c t g c a a c t t t a t c c g c c t c c a t c c a g t c t a t t a a t t g t t g c c g g g a a g c t a g a g t a a g a g g a c g t t g a a a t a g g c g g a g g t a g g t c a g a t a a t t a a c a a c g g c c c t t c g a t c t c a t t c

PstI 6240 195 190 185 180 LEGTLLKRLTTAMAAPMTTD AmpR LEGTLLKRLTTAMAAPMTTD t a g t t c g c c a g t t a a t a g t t t g c g c a a c g t t g t t g c c a t t g c t g c a g g c a t c g t g g t g t c a t c a a g c g g t c a a t t a t c a a a c g c g t t g c a a c a a c g g t a a c g a c g t c c g t a g c a c c a c a g

6300 175 170 165 160 REDNPIAENLEPEWRDLRTV AmpR REDNPIAENLEPEWRDLRTV a c g c t c g t c g t t t g g t a t g g c t t c a t t c a g c t c c g g t t c c c a a c g a t c a a g g c g a g t t a c t g c g a g c a g c a a a c c a t a c c g a a g t a a g t c g a g g c c a a g g g t t g c t a g t t c c g c t c a a t g

PvuI 6360 155 150 145 140 HDGMNHLFATLEKPGGITTL AmpR HDGMNHLFATLEKPGGITTL a t g a t c c c c c a t a t t g t g c a a a a a a g c g g t t a g c t c c t t c g g t c c t c c g a t c g t t g t c a g t a c t a g g g g g t a t a a c a c g t t t t t t c g c c a a t c g a g g a a g c c a g g a g g c t a g c a a c a g t c

6420 135 130 125 120 LLNAATNDSMTIAASCLERV AmpR LLNAATNDSMTIAASCLERV a a g t a a g t t g g c c g c a g t g t t a t c a c t c a t g g t t a t g g c a g c a c t g c a t a a t t c t c t t a c t t c a t t c a a c c g g c g t c a c a a t a g t g a g t a c c a a t a c c g t c g t g a c g t a t t a a g a g a a t g

6480 115 110 105 100 TMGDTLHKETVPSYEVLDNQ AmpR TMGDTLHKETVPSYEVLDNQ t g t c a t g c c a t c c g t a a g a t g c t t t t c t g t g a c t g g t g a g t a c t c a a c c a a g t c a t t c t g a c a g t a c g g t a g g c a t t c t a c g a a a a g a c a c t g a c c a c t c a t g a g t t g g t t c a g t a a g a c

6540 95 90 85 80 SYHIRRGLQEQGADVRSLVA AmpR A T A A T A C C G C Amp-R SYHIRRGLQEQGADVRSLVA a g a a t a g t g t a t g c g g c g a c c g a g t t g c t c t t g c c c g g c g t c a a c a c g g g a t a a t a c c g c t c t t a t c a c a t a c g c c g c t g g c t c a a c g a g a a c g g g c c g c a g t t g t g c c c t a t t a t g g c g

6600 75 70 65 60 GCLLVKFTSMMPFREEPRFS AmpR G C C A C A T A G C Amp-R GCLLVKFTSMMPFREEPRFS g c c a c a t a g c a g a a c t t t a a a a g t g c t c a t c a t t g g a a a a c g t t c t t c g g g g c g a a a a c t c g g t g t a t c g t c t t g a a a t t t t c a c g a g t a g t a a c c t t t t g c a a g a a g c c c c g c t t t t g a

6660 55 50 45 40 ELIKGSNLDLEIYGVRAGLQ AmpR ELIKGSNLDLEIYGVRAGLQ c t c a a g g a t c t t a c c g c t g t t g a g a t c c a g t t c g a t g t a a c c c a c t c g t g c a c c c a a c t g g a g t t c c t a g a a t g g c g a c a a c t c t a g g t c a a g c t a c a t t g g g t g a g c a c g t g g g t t g a c

6720 35 30 25 DEADKVKVLTEPH 20 signal sequence AFVPLCF AmpR DEADKVKVLTEPHAFVPLCF a t c t t c a g c a t c t t t t a c t t t c a c c a g c g t t t c t g g g t g a g c a a a a a c a g g a a g g c a a a a t a g a a g t c g t a g a a a a t g a a a g t g g t c g c a a a g a c c c a c t c g t t t t t g t c c t t c c g t t t t

6780 15 10 5 1 signal sequence AAFFPILAVRFHQISM AmpR AmpR promoter AAFFPILAVRFHQISM t g c c g c a a a a a a g g g a a t a a g g g c g a c a c g g a a a t g t t g a a t a c t c a t a c t c t t c c t t t t a c g g c g t t t t t t c c c t t a t t c c c g c t g t g c c t t t a c a a c t t a t g a g t a t g a g a a g g a a a a

6840 AmpR promoter t c a a t a t t a t t g a a g c a t t t a t c a g g g t t a t t g t c t c a t g a g c g g a t a c a t a t t t g a a t g a g t t a t a a t a a c t t c g t a a a t a g t c c c a a t a a c a g a g t a c t c g c c t a t g t a t a a a c t t a c

6900 AmpR promoter t a t t t a g a a a a a t a a a c a a a t a g g g g t t c c g c g c a c a t t t c c c c g a a a a g t g c c a c c t g a a t a a a t c t t t t t a t t t g t t t a t c c c c a a g g c g c g t g t a a a g g g g c t t t t c a c g g t g g a c t

ZraIAatII 3ʹ 5ʹ 6921 c g t c t a a g a a a c c a t t a t t a t g c a g a t t c t t t g g t a a t a a t a

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.