Addgene pMOD_A0908 Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: pMOD_A0908 Sequencing Result


Map View

RsrII (8216) AhdI (8042) NmeAIII (7895) Bsu36I (7116) BmgBI (7093) SacI (7081) Eco53kI (7079) AatII - AccI (6818) SalI - SgrDI (6817) ZraI (6816) BsaHI (6815) SV40 NLS HpaI (6374) AbsI (6219) KflI (6053) AgeI (5979) CsiI - SexAI * (5247) pBR322ori-F (569 .. 588) L4440 (822 .. 839) AscI - BssHII (872) BbsI (915) XbaI * (1323) BsmBI - Esp3I (1630) AfeI (1639) AclI (1697) SbfI (1895) DraIII (2086) PspOMI (2228) ApaI (2232) XcmI (2240) AleI (2279) MscI * (2418) PvuII (2554) PflMI (2589) NcoI (2674) pMOD_A0908 8242 bp

Sequence View

5ʹ 3ʹ 60 g c c t t g a c g g c a a a a t c c c t t a a c g t g a g t t t t c g t t c c a c t g a g c g t c a g a c c c c g t a g c g g a a c t g c c g t t t t a g g g a a t t g c a c t c a a a a g c a a g g t g a c t c g c a g t c t g g g g c a t c

120 ori a a a a g a t c a a a g g a t c t t c t t g a g a t c c t t t t t t t c t g c g c g t a a t c t g c t g c t t g c a a a t t t t c t a g t t t c c t a g a a g a a c t c t a g g a a a a a a a g a c g c g c a t t a g a c g a c g a a c g t t t

180 ori c a a a a a a a c c a c c g c t a c c a g c g g t g g t t t g t t t g c c g g a t c a a g a g c t a c t a a c t c t t t g t t t t t t t g g t g g c g a t g g t c g c c a c c a a a c a a a c g g c c t a g t t c t c g a t g a t t g a g a a a

240 ori t t c c g a a g g t a a c t g g c t t c a g c a g a g c g c a g a t a c c a a a t a c t g t t c t t c t a g t g t a g c a a g g c t t c c a t t g a c c g a a g t c g t c t c g c g t c t a t g g t t t a t g a c a a g a a g a t c a c a t c g

300 ori c g t a g t t a g g c c a c c a c t t c a a g a a c t c t g t a g c a c c g c c t a c a t a c c t c g c t c t g c t a a g c a t c a a t c c g g t g g t g a a g t t c t t g a g a c a t c g t g g c g g a t g t a t g g a g c g a g a c g a t t

360 ori t c c t g t t a c c a g t g g c t g c t g c c a g t g g c g a t a a g t c g t g t c t t a c c g g g t t g g a c t c a a a g g a c a a t g g t c a c c g a c g a c g g t c a c c g c t a t t c a g c a c a g a a t g g c c c a a c c t g a g t t

420 ori g a c g a t a g t t a c c g g a t a a g g c g c a g c g g t c g g g c t g a a c g g g g g g t t c g t g c t c a c a g c c t g c t a t c a a t g g c c t a t t c c g c g t c g c c a g c c c g a c t t g c c c c c c a a g c a c g a g t g t c g

480 ori c c a g c t t g g a g c g a a c g a c c t a c a c c g a a c t g a g a t a c c t a c a g c g t g a g c t a t g a g a a a g g t c g a a c c t c g c t t g c t g g a t g t g g c t t g a c t c t a t g g a t g t c g c a c t c g a t a c t c t t t

540 ori g c g c c a c g c t t c c c g a a g g g a g a a a g g c g g a c a g g t a t c c g g t a a g c g g c a g g g t c g g a a c g c g g t g c g a a g g g c t t c c c t c t t t c c g c c t g t c c a t a g g c c a t t c g c c g t c c c a g c c t t

600 ori G G G A A A C G C C T G G T A T C T T T pBR322ori-F c a g g a g a g c g c a c g a g g g a g c t t c c a g g g g g a a a c g c c t g g t a t c t t t a t a g t c c t g t c g g t c c t c t c g c g t g c t c c c t c g a a g g t c c c c c t t t g c g g a c c a t a g a a a t a t c a g g a c a g c

660 ori g g t t t c g c c a c c t c t g a c t t g a g c g t c g a t t t t t g t g a t g c t c g t c a g g g g g g c g g a g c c c c a a a g c g g t g g a g a c t g a a c t c g c a g c t a a a a a c a c t a c g a g c a g t c c c c c c g c c t c g g

720 ori t a t g g a a a a a c g c c a g c a a c g c g g c c t t t t t a c g g t t c c t g g c c t t t t g c t g g c c t t t t g a t a c c t t t t t g c g g t c g t t g c g c c g g a a a a a t g c c a a g g a c c g g a a a a c g a c c g g a a a a c

780 c t c a c a t g t t c t t t c c t g c g t t a t c c c c t g a t t c t g t g g a t a a c c g t a t t a c c g c c t t t g g a g t g t a c a a g a a a g g a c g c a a t a g g g g a c t a a g a c a c c t a t t g g c a t a a t g g c g g a a a c

840 A G C G A G T C A G T G A G C G A G L4440 a g t g a g c t g a t a c c g c t c g c c g c a g c c g a a c g a c c g a g c g c a g c g a g t c a g t g a g c g a g g t c a c t c g a c t a t g g c g a g c g g c g t c g g c t t g c t g g c t c g c g t c g c t c a g t c a c t c g c t c c

BssHIIAscI 900 a a g c g g a a g a g c g c c c a c c t g c t c a t g a t c g g c g c g c c a c t t t g a t a a a t g t t c c t c g c t t t c g c c t t c t c g c g g g t g g a c g a g t a c t a g c c g c g c g g t g a a a c t a t t t a c a a g g a g c g a

BbsI 960 g a c g t a a g a a g a c a t t a g t a a t g g t t a t a a t a t a t a g c t t t c t a t g a a t g t a t g g t g a g a c t g c a t t c t t c t g t a a t c a t t a c c a a t a t t a t a t a t c g a a a g a t a c t t a c a t a c c a c t c t

1020 a a a t g t c t g t t c a c t g a t t t t g a g t t t g g a a t a a a a g c a t t t g c g t t t g g t t t a t c a t t g t t t a c a g a c a a g t g a c t a a a a c t c a a a c c t t a t t t t c g t a a a c g c a a a c c a a a t a g t a a c

1080 c g t t t a t a c a a g g a c a g a g a t c c a c t g a g c t g g a a t a g c t t a a a a c c a t t a t c a g a a c a a g c a a a t a t g t t c c t g t c t c t a g g t g a c t c g a c c t t a t c g a a t t t t g g t a a t a g t c t t g t t

1140 a a t a a a c c a t t t t t t g t t a a g a a t c a g a g c a t a g t a a a c a a c a g a a a c a a c c t a a g a g a g t t a t t t g g t a a a a a a c a a t t c t t a g t c t c g t a t c a t t t g t t g t c t t t g t t g g a t t c t c t c

1200 g t a a c t t g t c c a a g a a g a t a g c t a a t t a t a t c t a t t t t a t a a a a g t t a t c a t a g t t t g t a c a t t g a a c a g g t t c t t c t a t c g a t t a a t a t a g a t a a a a t a t t t t c a a t a g t a t c a a a c a t

1260 a g t c a c a a a a g a t g c a a a t a a c a g a g a a a c t a g g a g a c t t g a g a a t a t a c a t t c t t g t a t t c a g t g t t t t c t a c g t t t a t t g t c t c t t t g a t c c t c t g a a c t c t t a t a t g t a a g a a c a t a

1320 a t t t g t a t t c g a g a t t g t g a a a a t t t g a c c a t a a g t t t a a a t t c t t a a a a a g a t a t a t c t t a a a c a t a a g c t c t a a c a c t t t t a a a c t g g t a t t c a a a t t t a a g a a t t t t t c t a t a t a g a

XbaI* 1380 g a t c t a g a t g a t g g t t a t a g a c t g t a a t t t t a c c a c a t g t t t a a t g a t g g a t a g t g a c a c c t a g a t c t a c t a c c a a t a t c t g a c a t t a a a a t g g t g t a c a a a t t a c t a c c t a t c a c t g t g

1440 a c a t g a c a c a t c g a c a a c a c t a t a g c a t c t t a t t t a g a t t a c a a c a t g a a a t t t t t c t g t t g t a c t g t g t a g c t g t t g t g a t a t c g t a g a a t a a a t c t a a t g t t g t a c t t t a a a a a g a c a

1500 a a t a c a t g t c t t t g t a c a t a a t t t a a a a g t a a t t c c t a a g a a a t a t a t t t a t a c a a g g a g t t a t g t a c a g a a a c a t g t a t t a a a t t t t c a t t a a g g a t t c t t t a t a t a a a t a t g t t c c t c

1560 t t t a a a g a a a a c a t a g c a t a a a g t t c a a t g a g t a g t a a a a a c c a t a t a c a g t a t a t a g c a a a a t t t c t t t t g t a t c g t a t t t c a a g t t a c t c a t c a t t t t t g g t a t a t g t c a t a t a t c g t

1620 t a a a g t t c a a t g a g t t t a t t a c a a a a g c a t t g g t t c a c t t t c t g t a a c a c g a c g t t a a a c a t t t c a a g t t a c t c a a a t a a t g t t t t c g t a a c c a a g t g a a a g a c a t t g t g c t g c a a t t t g

AfeIBsmBIEsp3I 1680 c t t c g t c t c c a a t a g g a g c g c t a c t g a t t c a a c a t g c c a a t a t a t a c t a a a t a c g t t t c t g a a g c a g a g g t t a t c c t c g c g a t g a c t a a g t t g t a c g g t t a t a t a t g a t t t a t g c a a a g a

AclI 1740 a c a g t c a a a t g c t t t a a c g t t t c a t g a t t a a g t g a c t a t t t a c c g t c a a t c c t t t c c c a t t g t c a g t t t a c g a a a t t g c a a a g t a c t a a t t c a c t g a t a a a t g g c a g t t a g g a a a g g g t a

1800 t c c t c c c a c t a a t c c a a c t t t t t a a t t a c t c t t a a a t c a c c a c t a a g c t t c g a a t c c a t c a g g a g g g t g a t t a g g t t g a a a a a t t a a t g a g a a t t t a g t g g t g a t t c g a a g c t t a g g t a g

1860 c a a a a c c a c a a t a t a a a a a c a g a a c t c t c g t a a c t c a a t c a t c g c a a a a c a a a a c a a a a c g t t t t g g t g t t a t a t t t t t g t c t t g a g a g c a t t g a g t t a g t a g c g t t t t g t t t t g t t t t g

SbfI 1920 MSEPPR a a a a c a a a a a c c c c a a a a a g a a a g a a t a a c c t g c a g g a a a a a t g t c t g a g c c a c c t c g g g t t t t g t t t t t g g g g t t t t t c t t t c t t a t t g g a c g t c c t t t t t a c a g a c t c g g t g g a g c c c

1980 AETFVFLDLEATGLPNMDPE c t g a g a c c t t t g t a t t c c t g g a c c t a g a a g c c a c t g g g c t c c c a a a c a t g g a c c c t g a g a g a c t c t g g a a a c a t a a g g a c c t g g a t c t t c g g t g a c c c g a g g g t t t g t a c c t g g g a c t c t

2040 IAEISLFAVHRSSLENPERD t t g c a g a g a t a t c c c t t t t t g c t g t t c a c c g c t c t t c c c t g g a g a a c c c a g a a c g g g a t g a a c g t c t c t a t a g g g a a a a a c g a c a a g t g g c g a g a a g g g a c c t c t t g g g t c t t g c c c t a c

DraIII 2100 DSGSLVLPRVLDKLTLCMCP a t t c t g g t t c c t t g g t g c t g c c c c g t g t t c t g g a c a a g c t c a c a c t g t g c a t g t g c c c g g t a a g a c c a a g g a a c c a c g a c g g g g c a c a a g a c c t g t t c g a g t g t g a c a c g t a c a c g g g c c

2160 ERPFTAKASEITGLSSESLM a g c g c c c c t t t a c t g c c a a g g c c a g t g a g a t t a c t g g t t t g a g c a g c g a a a g c c t g a t g c t c g c g g g g a a a t g a c g g t t c c g g t c a c t c t a a t g a c c a a a c t c g t c g c t t t c g g a c t a c g

2220 HCGKAGFNGAVVRTLQGFLS a c t g c g g g a a g g c t g g t t t c a a t g g c g c t g t g g t a a g g a c a c t g c a g g g c t t c c t a a g c c t g a c g c c c t t c c g a c c a a a g t t a c c g c g a c a c c a t t c c t g t g a c g t c c c g a a g g a t t c g g

AleIXcmIPspOMIApaI 2280 RQEGPICLVAHNGFDYDFPL g c c a g g a g g g c c c c a t c t g c c t t g t g g c c c a c a a t g g c t t c g a t t a t g a c t t c c c a c t g c c g g t c c t c c c g g g g t a g a c g g a a c a c c g g g t g t t a c c g a a g c t a a t a c t g a a g g g t g a c g

2340 LCTELQRLGAHLPQDTVCLD t g t g c a c g g a g c t a c a a c g t c t g g g t g c c c a t c t g c c c c a a g a c a c t g t c t g c c t g g a c a a c a c g t g c c t c g a t g t t g c a g a c c c a c g g g t a g a c g g g g t t c t g t g a c a g a c g g a c c t g t

2400 TLPALRGLDRAHSHGTRAQG c a c t g c c t g c a t t g c g g g g c c t g g a c c g t g c t c a c a g c c a c g g c a c c a g g g c t c a a g g c c g t g a c g g a c g t a a c g c c c c g g a c c t g g c a c g a g t g t c g g t g c c g t g g t c c c g a g t t c c g g

MscI* 2460 RKSYSLASLFHRYFQAEPSA g c a a a a g c t a c a g c c t g g c c a g t c t c t t c c a c c g c t a c t t c c a g g c t g a a c c c a g t g c t g c g t t t t c g a t g t c g g a c c g g t c a g a g a a g g t g g c g a t g a a g g t c c g a c t t g g g t c a c g a c

2520 AHSAEGDVHTLLLIFLHRAP c c c a t t c a g c a g a a g g t g a t g t g c a c a c c c t g c t t c t g a t c t t c c t g c a t c g t g c t c c t g g g g t a a g t c g t c t t c c a c t a c a c g t g t g g g a c g a a g a c t a g a a g g a c g t a g c a c g a g g a c

PvuII 2580 ELLAWADEQARSWAHIEPMY a g c t g c t c g c c t g g g c a g a t g a g c a g g c c c g c a g c t g g g c t c a t a t t g a g c c c a t g t a c g t c g a c g a g c g g a c c c g t c t a c t c g t c c g g g c g t c g a c c c g a g t a t a a c t c g g g t a c a t g c

PflMI 2640 1 5 ATNFSLL P2A VPPDGPSLEAGSGATNFSLL t g c c a c c t g a t g g t c c a a g c c t c g a a g c c g g a t c t g g a g c t a c t a a t t t t t c t c t t c t t a a c g g t g g a c t a c c a g g t t c g g a g c t t c g g c c t a g a c c t c g a t g a t t a a a a a g a g a a g a a t

NcoI 2700 10 15 KQAGDVEENPGP 1 5 MDKKYSIG P2A Cas9 1 5 MDKKYSIG Cas9 KQAGDVEENPGPMDKKYSIG a g c a a g c t g g a g a t g t t g a a g a a a a t c c t g g a c c c a t g g a t a a g a a g t a c t c t a t c g g a c t c g t t c g a c c t c t a c a a c t t c t t t t a g g a c c t g g g t a c c t a t t c t t c a t g a g a t a g c c t g

2760 10 15 20 25 LDIGTNSVGWAVITDEYKVP Cas9 10 15 20 25 LDIGTNSVGWAVITDEYKVP Cas9 LDIGTNSVGWAVITDEYKVP t c g a t a t c g g a a c t a a c t c t g t g g g a t g g g c t g t g a t c a c c g a t g a g t a c a a g g t g c c a t a g c t a t a g c c t t g a t t g a g a c a c c c t a c c c g a c a c t a g t g g c t a c t c a t g t t c c a c g g t a

2820 30 35 40 45 SKKFKVLGNTDRHSIKKNLI Cas9 30 35 40 45 SKKFKVLGNTDRHSIKKNLI Cas9 SKKFKVLGNTDRHSIKKNLI c t a a g a a g t t c a a g g t t c t c g g a a a c a c c g a t a g g c a c t c t a t c a a g a a a a a c c t t a t c g g a t t c t t c a a g t t c c a a g a g c c t t t g t g g c t a t c c g t g a g a t a g t t c t t t t t g g a a t a g c

2880 50 55 60 65 GALLFDSGETAEATRLKRTA Cas9 50 55 60 65 GALLFDSGETAEATRLKRTA Cas9 GALLFDSGETAEATRLKRTA g t g c t c t c c t c t t c g a t t c t g g t g a a a c t g c t g a g g c t a c c a g a c t c a a g a g a a c c g c t a c a c g a g a g g a g a a g c t a a g a c c a c t t t g a c g a c t c c g a t g g t c t g a g t t c t c t t g g c g a t

2940 70 75 80 85 RRRYTRRKNRICYLQEIFSN Cas9 70 75 80 85 RRRYTRRKNRICYLQEIFSN Cas9 RRRYTRRKNRICYLQEIFSN g a a g a a g g t a c a c c a g a a g a a a g a a c a g g a t c t g c t a c c t c c a a g a g a t c t t c t c t a a c g c t t c t t c c a t g t g g t c t t c t t t c t t g t c c t a g a c g a t g g a g g t t c t c t a g a a g a g a t t g c

3000 90 95 100 105 EMAKVDDSFFHRLEESFLVE Cas9 90 95 100 105 EMAKVDDSFFHRLEESFLVE Cas9 EMAKVDDSFFHRLEESFLVE a g a t g g c t a a a g t g g a t g a t t c a t t c t t c c a c a g g c t c g a a g a g t c a t t c c t c g t g g a a g t c t a c c g a t t t c a c c t a c t a a g t a a g a a g g t g t c c g a g c t t c t c a g t a a g g a g c a c c t t c

3060 110 115 120 125 EDKKHERHPIFGNIVDEVAY Cas9 110 115 120 125 EDKKHERHPIFGNIVDEVAY Cas9 EDKKHERHPIFGNIVDEVAY a a g a t a a g a a g c a c g a g a g g c a c c c t a t c t t c g g a a a c a t c g t t g a t g a g g t g g c a t a c c t t c t a t t c t t c g t g c t c t c c g t g g g a t a g a a g c c t t t g t a g c a a c t a c t c c a c c g t a t g g

3120 130 135 140 145 HEKYPTIYHLRKKLVDSTDK Cas9 130 135 140 145 HEKYPTIYHLRKKLVDSTDK Cas9 HEKYPTIYHLRKKLVDSTDK a c g a g a a g t a c c c t a c t a t c t a c c a c c t c a g a a a g a a g c t c g t t g a t t c t a c t g a t a a g g t g c t c t t c a t g g g a t g a t a g a t g g t g g a g t c t t t c t t c g a g c a a c t a a g a t g a c t a t t c c

3180 150 155 160 165 ADLRLIYLALAHMIKFRGHF Cas9 150 155 160 165 ADLRLIYLALAHMIKFRGHF Cas9 ADLRLIYLALAHMIKFRGHF c t g a t c t c a g g c t c a t c t a c c t c g c t c t c g c t c a c a t g a t c a a g t t c a g a g g a c a c t t c c g a c t a g a g t c c g a g t a g a t g g a g c g a g a g c g a g t g t a c t a g t t c a a g t c t c c t g t g a a g g

3240 170 175 180 185 LIEGDLNPDNSDVDKLFIQL Cas9 170 175 180 185 LIEGDLNPDNSDVDKLFIQL Cas9 LIEGDLNPDNSDVDKLFIQL t c a t c g a g g g t g a t c t c a a c c c t g a t a a c t c t g a t g t g g a t a a g t t g t t c a t c c a g c t c g a g t a g c t c c c a c t a g a g t t g g g a c t a t t g a g a c t a c a c c t a t t c a a c a a g t a g g t c g a g c

3300 190 195 200 205 VQTYNQLFEENPINASGVDA Cas9 190 195 200 205 VQTYNQLFEENPINASGVDA Cas9 VQTYNQLFEENPINASGVDA t g c a g a c c t a c a a c c a g c t t t t c g a a g a g a a c c c t a t c a a c g c t t c a g g t g t g g a t g c t a a c g t c t g g a t g t t g g t c g a a a a g c t t c t c t t g g g a t a g t t g c g a a g t c c a c a c c t a c g a t

3360 210 215 220 225 KAILSARLSKSRRLENLIAQ Cas9 210 215 220 225 KAILSARLSKSRRLENLIAQ Cas9 KAILSARLSKSRRLENLIAQ a g g c t a t c c t c t c t g c t a g g c t c t c t a a g t c a a g a a g g c t t g a g a a c c t c a t t g c t c a g c t c c g a t a g g a g a g a c g a t c c g a g a g a t t c a g t t c t t c c g a a c t c t t g g a g t a a c g a g t c g

3420 230 235 240 245 LPGEKKNGLFGNLIALSLGL Cas9 230 235 240 245 LPGEKKNGLFGNLIALSLGL Cas9 LPGEKKNGLFGNLIALSLGL t c c c t g g t g a g a a g a a g a a c g g a c t t t t c g g a a a c t t g a t c g c t c t c t c t c t c g g a c t c a a g g g a c c a c t c t t c t t c t t g c c t g a a a a g c c t t t g a a c t a g c g a g a g a g a g a g c c t g a g t

3480 250 255 260 265 TPNFKSNFDLAEDAKLQLSK Cas9 250 255 260 265 TPNFKSNFDLAEDAKLQLSK Cas9 TPNFKSNFDLAEDAKLQLSK c c c c t a a c t t c a a g t c t a a c t t c g a t c t c g c t g a g g a t g c a a a g c t c c a g c t c t c a a a g g g g g g a t t g a a g t t c a g a t t g a a g c t a g a g c g a c t c c t a c g t t t c g a g g t c g a g a g t t t c c

3540 270 275 280 285 DTYDDDLDNLLAQIGDQYAD Cas9 270 275 280 285 DTYDDDLDNLLAQIGDQYAD Cas9 DTYDDDLDNLLAQIGDQYAD a t a c c t a c g a t g a t g a t c t c g a t a a c c t c c t c g c t c a g a t c g g a g a t c a g t a c g c t g a t t t a t g g a t g c t a c t a c t a g a g c t a t t g g a g g a g c g a g t c t a g c c t c t a g t c a t g c g a c t a a

3600 290 295 300 305 LFLAAKNLSDAILLSDILRV Cas9 290 295 300 305 LFLAAKNLSDAILLSDILRV Cas9 LFLAAKNLSDAILLSDILRV t g t t c c t c g c t g c t a a g a a c c t c t c t g a t g c t a t c c t c c t c a g t g a t a t c c t c a g a g t g a a c a a g g a g c g a c g a t t c t t g g a g a g a c t a c g a t a g g a g g a g t c a c t a t a g g a g t c t c a c t

3660 310 315 320 325 NTEITKAPLSASMIKRYDEH Cas9 310 315 320 325 NTEITKAPLSASMIKRYDEH Cas9 NTEITKAPLSASMIKRYDEH a c a c c g a g a t c a c c a a g g c t c c a c t c t c a g c t t c t a t g a t c a a g a g a t a c g a t g a g c a c c t g t g g c t c t a g t g g t t c c g a g g t g a g a g t c g a a g a t a c t a g t t c t c t a t g c t a c t c g t g g

3720 330 335 340 345 HQDLTLLKALVRQQLPEKYK Cas9 330 335 340 345 HQDLTLLKALVRQQLPEKYK Cas9 HQDLTLLKALVRQQLPEKYK a c c a g g a t c t c a c a c t t c t c a a g g c t c t t g t t a g a c a g c a g c t c c c a g a g a a g t a c a a a g t g g t c c t a g a g t g t g a a g a g t t c c g a g a a c a a t c t g t c g t c g a g g g t c t c t t c a t g t t t c

3780 350 355 360 365 EIFFDQSKNGYAGYIDGGAS Cas9 350 355 360 365 EIFFDQSKNGYAGYIDGGAS Cas9 EIFFDQSKNGYAGYIDGGAS a g a t t t t c t t c g a t c a g t c t a a g a a c g g a t a c g c t g g t t a c a t c g a t g g t g g t g c a t c t c t c t a a a a g a a g c t a g t c a g a t t c t t g c c t a t g c g a c c a a t g t a g c t a c c a c c a c g t a g a g

3840 370 375 380 385 QEEFYKFIKPILEKMDGTEE Cas9 370 375 380 385 QEEFYKFIKPILEKMDGTEE Cas9 QEEFYKFIKPILEKMDGTEE a a g a a g a g t t c t a c a a g t t c a t c a a g c c t a t c c t c g a g a a g a t g g a t g g a a c c g a g g a a c t t c t t c t c a a g a t g t t c a a g t a g t t c g g a t a g g a g c t c t t c t a c c t a c c t t g g c t c c t t g

3900 390 395 400 405 LLVKLNREDLLRKQRTFDNG Cas9 390 395 400 405 LLVKLNREDLLRKQRTFDNG Cas9 LLVKLNREDLLRKQRTFDNG t c c t c g t g a a g c t c a a t a g a g a g g a t c t t c t c a g a a a g c a g a g g a c c t t c g a t a a c g g a t a g g a g c a c t t c g a g t t a t c t c t c c t a g a a g a g t c t t t c g t c t c c t g g a a g c t a t t g c c t a

3960 410 415 420 425 SIPHQIHLGELHAILRRQED Cas9 410 415 420 425 SIPHQIHLGELHAILRRQED Cas9 SIPHQIHLGELHAILRRQED c t a t c c c t c a t c a g a t c c a c c t c g g a g a g t t g c a c g c t a t c c t t a g a a g g c a a g a g g a t t g a t a g g g a g t a g t c t a g g t g g a g c c t c t c a a c g t g c g a t a g g a a t c t t c c g t t c t c c t a a

4020 430 435 440 445 FYPFLKDNREKIEKILTFRI Cas9 430 435 440 445 FYPFLKDNREKIEKILTFRI Cas9 FYPFLKDNREKIEKILTFRI t c t a c c c a t t c c t c a a g g a t a a c a g g g a a a a g a t t g a g a a g a t t c t c a c c t t c a g a a t c c a g a t g g g t a a g g a g t t c c t a t t g t c c c t t t t c t a a c t c t t c t a a g a g t g g a a g t c t t a g g

4080 450 455 460 465 PYYVGPLARGNSRFAWMTRK Cas9 450 455 460 465 PYYVGPLARGNSRFAWMTRK Cas9 PYYVGPLARGNSRFAWMTRK c t t a c t a c g t g g g a c c t c t c g c t a g a g g a a a c t c a a g a t t c g c t t g g a t g a c c a g a a a g t g a a t g a t g c a c c c t g g a g a g c g a t c t c c t t t g a g t t c t a a g c g a a c c t a c t g g t c t t t c a

4140 470 475 480 485 SEETITPWNFEEVVDKGASA Cas9 470 475 480 485 SEETITPWNFEEVVDKGASA Cas9 SEETITPWNFEEVVDKGASA c t g a g g a a a c c a t c a c c c c t t g g a a c t t c g a a g a g g t g g t g g a t a a g g g t g c t a g t g c t c g a c t c c t t t g g t a g t g g g g a a c c t t g a a g c t t c t c c a c c a c c t a t t c c c a c g a t c a c g a g

4200 490 495 500 505 QSFIERMTNFDKNLPNEKVL Cas9 490 495 500 505 QSFIERMTNFDKNLPNEKVL Cas9 QSFIERMTNFDKNLPNEKVL a g t c t t t c a t c g a g a g g a t g a c c a a c t t c g a t a a g a a c c t t c c a a a c g a g a a g g t g c t c c t c a g a a a g t a g c t c t c c t a c t g g t t g a a g c t a t t c t t g g a a g g t t t g c t c t t c c a c g a g g

4260 510 515 520 525 PKHSLLYEYFTVYNELTKVK Cas9 510 515 520 525 PKHSLLYEYFTVYNELTKVK Cas9 PKHSLLYEYFTVYNELTKVK c t a a g c a c t c t t t g c t c t a c g a g t a c t t c a c c g t g t a c a a c g a g t t g a c c a a g g t t a a g t g a t t c g t g a g a a a c g a g a t g c t c a t g a a g t g g c a c a t g t t g c t c a a c t g g t t c c a a t t c a

4320 530 535 540 545 YVTEGMRKPAFLSGEQKKAI Cas9 530 535 540 545 YVTEGMRKPAFLSGEQKKAI Cas9 YVTEGMRKPAFLSGEQKKAI a c g t g a c c g a g g g a a t g a g g a a g c c t g c t t t t t t g t c a g g t g a g c a a a a g a a g g c t a t c g t g c a c t g g c t c c c t t a c t c c t t c g g a c g a a a a a a c a g t c c a c t c g t t t t c t t c c g a t a g c

4380 550 555 560 565 VDLLFKTNRKVTVKQLKEDY Cas9 550 555 560 565 VDLLFKTNRKVTVKQLKEDY Cas9 VDLLFKTNRKVTVKQLKEDY t t g a t c t c t t g t t c a a g a c c a a c a g a a a g g t g a c c g t g a a g c a g c t c a a a g a g g a t t a c t a a c t a g a g a a c a a g t t c t g g t t g t c t t t c c a c t g g c a c t t c g t c g a g t t t c t c c t a a t g a

4440 570 575 580 585 FKKIECFDSVEISGVEDRFN Cas9 570 575 580 585 FKKIECFDSVEISGVEDRFN Cas9 FKKIECFDSVEISGVEDRFN t c a a g a a a a t c g a g t g c t t c g a t t c a g t t g a g a t t t c t g g t g t t g a g g a t a g g t t c a a c g a g t t c t t t t a g c t c a c g a a g c t a a g t c a a c t c t a a a g a c c a c a a c t c c t a t c c a a g t t g c

4500 590 595 600 605 ASLGTYHDLLKIIKDKDFLD Cas9 590 595 600 605 ASLGTYHDLLKIIKDKDFLD Cas9 ASLGTYHDLLKIIKDKDFLD c a t c t c t c g g a a c c t a c c a c g a t c t c c t c a a g a t c a t t a a g g a t a a g g a t t t c t t g g a t a g t a g a g a g c c t t g g a t g g t g c t a g a g g a g t t c t a g t a a t t c c t a t t c c t a a a g a a c c t a t

4560 610 615 620 625 NEENEDILEDIVLTLTLFED Cas9 610 615 620 625 NEENEDILEDIVLTLTLFED Cas9 NEENEDILEDIVLTLTLFED a c g a g g a a a a c g a g g a t a t c t t g g a g g a t a t c g t t c t t a c c c t c a c c c t c t t t g a a g a t a t g c t c c t t t t g c t c c t a t a g a a c c t c c t a t a g c a a g a a t g g g a g t g g g a g a a a c t t c t a t

4620 630 635 640 645 REMIEERLKTYAHLFDDKVM Cas9 630 635 640 645 REMIEERLKTYAHLFDDKVM Cas9 REMIEERLKTYAHLFDDKVM g a g a g a t g a t t g a a g a a a g g c t c a a g a c c t a c g c t c a t c t c t t c g a t g a t a a g g t g a t g a c t c t c t a c t a a c t t c t t t c c g a g t t c t g g a t g c g a g t a g a g a a g c t a c t a t t c c a c t a c t

4680 650 655 660 665 KQLKRRRYTGWGRLSRKLIN Cas9 650 655 660 665 KQLKRRRYTGWGRLSRKLIN Cas9 KQLKRRRYTGWGRLSRKLIN a g c a g t t g a a g a g a a g a a g a t a c a c t g g t t g g g g a a g g c t c t c a a g a a a g c t c a t t a a c g t c g t c a a c t t c t c t t c t t c t a t g t g a c c a a c c c c t t c c g a g a g t t c t t t c g a g t a a t t g c

4740 670 675 680 685 GIRDKQSGKTILDFLKSDGF Cas9 670 675 680 685 GIRDKQSGKTILDFLKSDGF Cas9 GIRDKQSGKTILDFLKSDGF g a a t c a g g g a t a a g c a g t c t g g a a a g a c a a t c c t t g a t t t c c t c a a g t c t g a t g g a t t c g c t t a g t c c c t a t t c g t c a g a c c t t t c t g t t a g g a a c t a a a g g a g t t c a g a c t a c c t a a g c

4800 690 695 700 705 ANRNFMQLIHDDSLTFKEDI Cas9 690 695 700 705 ANRNFMQLIHDDSLTFKEDI Cas9 ANRNFMQLIHDDSLTFKEDI c t a a c a g a a a c t t c a t g c a g c t c a t c c a c g a t g a t t c t c t c a c c t t t a a a g a g g a t a t c c g a t t g t c t t t g a a g t a c g t c g a g t a g g t g c t a c t a a g a g a g t g g a a a t t t c t c c t a t a g g

4860 710 715 720 725 QKAQVSGQGDSLHEHIANLA Cas9 710 715 720 725 QKAQVSGQGDSLHEHIANLA Cas9 QKAQVSGQGDSLHEHIANLA a g a a g g c t c a g g t t t c a g g a c a g g g t g a t a g t c t c c a t g a g c a t a t c g c t a a c c t c g c t g t c t t c c g a g t c c a a a g t c c t g t c c c a c t a t c a g a g g t a c t c g t a t a g c g a t t g g a g c g a c

4920 730 735 740 745 GSPAIKKGILQTVKVVDELV Cas9 730 735 740 745 GSPAIKKGILQTVKVVDELV Cas9 GSPAIKKGILQTVKVVDELV g a t c t c c t g c a a t c a a g a a g g g a a t c c t c c a g a c t g t g a a g g t t g t g g a t g a g t t g g t g a c t a g a g g a c g t t a g t t c t t c c c t t a g g a g g t c t g a c a c t t c c a a c a c c t a c t c a a c c a c t

4980 750 755 760 765 KVMGRHKPENIVIEMARENQ Cas9 750 755 760 765 KVMGRHKPENIVIEMARENQ Cas9 KVMGRHKPENIVIEMARENQ a g g t g a t g g g a a g g c a t a a g c c t g a g a a c a t c g t g a t c g a a a t g g c t a g a g a g a a c c a g a t c c a c t a c c c t t c c g t a t t c g g a c t c t t g t a g c a c t a g c t t t a c c g a t c t c t c t t g g t c t

5040 770 775 780 785 TTQKGQKNSRERMKRIEEGI Cas9 770 775 780 785 TTQKGQKNSRERMKRIEEGI Cas9 TTQKGQKNSRERMKRIEEGI c c a c t c a g a a g g g a c a g a a g a a c t c t a g g g a a a g g a t g a a g a g g a t c g a g g a a g g t a t c a g g t g a g t c t t c c c t g t c t t c t t g a g a t c c c t t t c c t a c t t c t c c t a g c t c c t t c c a t a g t

5100 790 795 800 805 KELGSQILKEHPVENTQLQN Cas9 790 795 800 805 KELGSQILKEHPVENTQLQN Cas9 KELGSQILKEHPVENTQLQN a a g a g c t t g g a t c t c a g a t c c t c a a a g a g c a c c c t g t t g a g a a c a c t c a g c t c c a g a a t g t t c t c g a a c c t a g a g t c t a g g a g t t t c t c g t g g g a c a a c t c t t g t g a g t c g a g g t c t t a c

5160 810 815 820 825 EKLYLYYLQNGRDMYVDQEL Cas9 810 815 820 825 EKLYLYYLQNGRDMYVDQEL Cas9 EKLYLYYLQNGRDMYVDQEL a g a a g c t c t a c c t c t a c t a c c t c c a g a a c g g a a g g g a t a t g t a t g t g g a t c a a g a g t t g g t c t t c g a g a t g g a g a t g a t g g a g g t c t t g c c t t c c c t a t a c a t a c a c c t a g t t c t c a a c c

5220 830 835 840 845 DINRLSDYDVDHIVPQSFLK Cas9 830 835 840 845 DINRLSDYDVDHIVPQSFLK Cas9 DINRLSDYDVDHIVPQSFLK a t a t c a a c a g g c t c t c t g a t t a c g a t g t t g a t c a t a t c g t g c c a c a g t c a t t c t t g a a g g t a t a g t t g t c c g a g a g a c t a a t g c t a c a a c t a g t a t a g c a c g g t g t c a g t a a g a a c t t c c

SexAI*CsiI 5280 850 855 860 865 DDSIDNKVLTRSDKNRGKSD Cas9 850 855 860 865 DDSIDNKVLTRSDKNRGKSD Cas9 DDSIDNKVLTRSDKNRGKSD a t g a t t c t a t c g a t a a c a a g g t g c t c a c c a g g t c t g a t a a g a a c a g g g g t a a g a g t g a t a t a c t a a g a t a g c t a t t g t t c c a c g a g t g g t c c a g a c t a t t c t t g t c c c c a t t c t c a c t a t

5340 870 875 880 885 NVPSEEVVKKMKNYWRQLLN Cas9 870 875 880 885 NVPSEEVVKKMKNYWRQLLN Cas9 NVPSEEVVKKMKNYWRQLLN a c g t g c c a a g t g a a g a g g t t g t g a a g a a a a t g a a g a a c t a t t g g a g g c a g c t c c t c a a c g t g c a c g g t t c a c t t c t c c a a c a c t t c t t t t a c t t c t t g a t a a c c t c c g t c g a g g a g t t g c

5400 890 895 900 905 AKLITQRKFDNLTKAERGGL Cas9 890 895 900 905 AKLITQRKFDNLTKAERGGL Cas9 AKLITQRKFDNLTKAERGGL c t a a g c t c a t c a c t c a g a g a a a g t t c g a t a a c t t g a c t a a g g c t g a g a g g g g a g g a c t c t g a t t c g a g t a g t g a g t c t c t t t c a a g c t a t t g a a c t g a t t c c g a c t c t c c c c t c c t g a g a

5460 910 915 920 925 SELDKAGFIKRQLVETRQIT Cas9 910 915 920 925 SELDKAGFIKRQLVETRQIT Cas9 SELDKAGFIKRQLVETRQIT c t g a a t t g g a t a a g g c a g g a t t c a t c a a g a g g c a g c t t g t g g a a a c c a g g c a g a t c a c t a g a c t t a a c c t a t t c c g t c c t a a g t a g t t c t c c g t c g a a c a c c t t t g g t c c g t c t a g t g a t

5520 930 935 940 945 KHVAQILDSRMNTKYDENDK Cas9 930 935 940 945 KHVAQILDSRMNTKYDENDK Cas9 KHVAQILDSRMNTKYDENDK a g c a c g t t g c a c a g a t c c t c g a t t c t a g g a t g a a c a c c a a g t a c g a t g a g a a c g a t a a g t t c g t g c a a c g t g t c t a g g a g c t a a g a t c c t a c t t g t g g t t c a t g c t a c t c t t g c t a t t c a

5580 950 955 960 965 LIREVKVITLKSKLVSDFRK Cas9 950 955 960 965 LIREVKVITLKSKLVSDFRK Cas9 LIREVKVITLKSKLVSDFRK t g a t c a g g g a a g t g a a g g t t a t c a c c c t c a a g t c a a a g c t c g t g t c t g a t t t c a g a a a g g a c t a g t c c c t t c a c t t c c a a t a g t g g g a g t t c a g t t t c g a g c a c a g a c t a a a g t c t t t c c

5640 970 975 980 985 DFQFYKVREINNYHHAHDAY Cas9 970 975 980 985 DFQFYKVREINNYHHAHDAY Cas9 DFQFYKVREINNYHHAHDAY a t t t c c a a t t c t a c a a g g t g a g g g a a a t c a a c a a c t a c c a c c a c g c t c a c g a t g c t t a c c t a a a g g t t a a g a t g t t c c a c t c c c t t t a g t t g t t g a t g g t g g t g c g a g t g c t a c g a a t g g

5700 990 995 1000 1005 LNAVVGTALIKKYPKLESEF Cas9 990 995 1000 1005 LNAVVGTALIKKYPKLESEF Cas9 LNAVVGTALIKKYPKLESEF t t a a c g c t g t t g t t g g a a c c g c t c t c a t c a a g a a g t a t c c t a a g c t c g a g t c a g a g t t c g a a t t g c g a c a a c a a c c t t g g c g a g a g t a g t t c t t c a t a g g a t t c g a g c t c a g t c t c a a g c

5760 1010 1015 1020 1025 VYGDYKVYDVRKMIAKSEQE Cas9 1010 1015 1020 1025 VYGDYKVYDVRKMIAKSEQE Cas9 VYGDYKVYDVRKMIAKSEQE t g t a c g g t g a t t a c a a g g t g t a c g a t g t g a g g a a g a t g a t c g c t a a g t c t g a g c a a g a g a a c a t g c c a c t a a t g t t c c a c a t g c t a c a c t c c t t c t a c t a g c g a t t c a g a c t c g t t c t c t

5820 1030 1035 1040 1045 IGKATAKYFFYSNIMNFFKT Cas9 1030 1035 1040 1045 IGKATAKYFFYSNIMNFFKT Cas9 IGKATAKYFFYSNIMNFFKT t c g g a a a g g c t a c c g c t a a g t a t t t c t t c t a c t c t a a c a t c a t g a a t t t c t t c a a g a c c g a g c c t t t c c g a t g g c g a t t c a t a a a g a a g a t g a g a t t g t a g t a c t t a a a g a a g t t c t g g c

5880 1050 1055 1060 1065 EITLANGEIRKRPLIETNGE Cas9 1050 1055 1060 1065 EITLANGEIRKRPLIETNGE Cas9 EITLANGEIRKRPLIETNGE a g a t t a c c c t c g c t a a c g g t g a g a t c a g a a a g a g g c c a c t c a t c g a g a c a a a c g g t g a a a t c t a a t g g g a g c g a t t g c c a c t c t a g t c t t t c t c c g g t g a g t a g c t c t g t t t g c c a c t t t

5940 1070 1075 1080 1085 TGEIVWDKGRDFATVRKVLS Cas9 1070 1075 1080 1085 TGEIVWDKGRDFATVRKVLS Cas9 TGEIVWDKGRDFATVRKVLS c a g g t g a g a t c g t g t g g g a t a a g g g a a g g g a t t t c g c t a c c g t t a g a a a g g t g c t c t c t a g t c c a c t c t a g c a c a c c c t a t t c c c t t c c c t a a a g c g a t g g c a a t c t t t c c a c g a g a g a t

AgeI 6000 1090 1095 1100 1105 MPQVNIVKKTEVQTGGFSKE Cas9 1090 1095 1100 1105 MPQVNIVKKTEVQTGGFSKE Cas9 MPQVNIVKKTEVQTGGFSKE t g c c a c a g g t g a a c a t c g t t a a g a a a a c c g a g g t g c a g a c c g g t g g a t t c t c t a a a g a g t a c g g t g t c c a c t t g t a g c a a t t c t t t t g g c t c c a c g t c t g g c c a c c t a a g a g a t t t c t c a

KflI 6060 1110 1115 1120 1125 SILPKRNSDKLIARKKDWDP Cas9 1110 1115 1120 1125 SILPKRNSDKLIARKKDWDP Cas9 SILPKRNSDKLIARKKDWDP c t a t c c t c c c t a a g a g g a a c t c t g a t a a g c t c a t t g c t a g g a a g a a g g a t t g g g a c c c t a g a t a g g a g g g a t t c t c c t t g a g a c t a t t c g a g t a a c g a t c c t t c t t c c t a a c c c t g g g a t

6120 1130 1135 1140 1145 KKYGGFDSPTVAYSVLVVAK Cas9 1130 1135 1140 1145 KKYGGFDSPTVAYSVLVVAK Cas9 KKYGGFDSPTVAYSVLVVAK a g a a a t a c g g t g g t t t c g a t t c t c c t a c c g t g g c t t a c t c t g t t c t c g t t g t g g c t a a g g t c t t t a t g c c a c c a a a g c t a a g a g g a t g g c a c c g a a t g a g a c a a g a g c a a c a c c g a t t c c

6180 1150 1155 1160 1165 VEKGKSKKLKSVKELLGITI Cas9 1150 1155 1160 1165 VEKGKSKKLKSVKELLGITI Cas9 VEKGKSKKLKSVKELLGITI t t g a g a a g g g a a a g a g t a a g a a g c t c a a g t c t g t t a a g g a a c t t c t c g g a a t c a c t a t c a a a c t c t t c c c t t t c t c a t t c t t c g a g t t c a g a c a a t t c c t t g a a g a g c c t t a g t g a t a g t

AbsI 6240 1170 1175 1180 1185 MERSSFEKNPIDFLEAKGYK Cas9 1170 1175 1180 1185 MERSSFEKNPIDFLEAKGYK Cas9 MERSSFEKNPIDFLEAKGYK t g g a a a g g t c a t c t t t c g a g a a g a a c c c a a t c g a t t t c c t c g a g g c t a a g g g a t a c a a a g a c c t t t c c a g t a g a a a g c t c t t c t t g g g t t a g c t a a a g g a g c t c c g a t t c c c t a t g t t t c

6300 1190 1195 1200 1205 EVKKDLIIKLPKYSLFELEN Cas9 1190 1195 1200 1205 EVKKDLIIKLPKYSLFELEN Cas9 EVKKDLIIKLPKYSLFELEN a g g t t a a g a a g g a t c t c a t c a t c a a g c t c c c a a a g t a c t c a c t c t t c g a a c t c g a g a a c g t c c a a t t c t t c c t a g a g t a g t a g t t c g a g g g t t t c a t g a g t g a g a a g c t t g a g c t c t t g c

6360 1210 1215 1220 1225 GRKRMLASAGELQKGNELAL Cas9 1210 1215 1220 1225 GRKRMLASAGELQKGNELAL Cas9 GRKRMLASAGELQKGNELAL g t a g a a a g a g g a t g c t c g c t t c t g c t g g t g a g c t t c a a a a g g g a a a c g a g c t t g c t c t c c c a t c t t t c t c c t a c g a g c g a a g a c g a c c a c t c g a a g t t t t c c c t t t g c t c g a a c g a g a g g

HpaI 6420 1230 1235 1240 1245 PSKYVNFLYLASHYEKLKGS Cas9 1230 1235 1240 1245 PSKYVNFLYLASHYEKLKGS Cas9 PSKYVNFLYLASHYEKLKGS c a t c t a a g t a c g t t a a c t t t c t t t a c c t c g c t t c t c a c t a c g a g a a g t t g a a g g g a t c t c g t a g a t t c a t g c a a t t g a a a g a a a t g g a g c g a a g a g t g a t g c t c t t c a a c t t c c c t a g a g

6480 1250 1255 1260 1265 PEDNEQKQLFVEQHKHYLDE Cas9 1250 1255 1260 1265 PEDNEQKQLFVEQHKHYLDE Cas9 PEDNEQKQLFVEQHKHYLDE c a g a a g a t a a c g a g c a g a a g c a a c t t t t c g t t g a g c a g c a c a a g c a c t a c t t g g a t g a g a g t c t t c t a t t g c t c g t c t t c g t t g a a a a g c a a c t c g t c g t g t t c g t g a t g a a c c t a c t c t

6540 1270 1275 1280 1285 IIEQISEFSKRVILADANLD Cas9 1270 1275 1280 1285 IIEQISEFSKRVILADANLD Cas9 IIEQISEFSKRVILADANLD t c a t c g a g c a g a t c t c t g a g t t c t c t a a a a g g g t g a t c c t c g c t g a t g c a a a c c t c g a t a a g t a g c t c g t c t a g a g a c t c a a g a g a t t t t c c c a c t a g g a g c g a c t a c g t t t g g a g c t a t

6600 1290 1295 1300 1305 KVLSAYNKHRDKPIREQAEN Cas9 1290 1295 1300 1305 KVLSAYNKHRDKPIREQAEN Cas9 KVLSAYNKHRDKPIREQAEN a g g t g t t g t c t g c t t a c a a c a a g c a c a g a g a t a a g c c t a t c a g g g a a c a g g c a g a g a a c a t c c a c a a c a g a c g a a t g t t g t t c g t g t c t c t a t t c g g a t a g t c c c t t g t c c g t c t c t t g t

6660 1310 1315 1320 1325 IIHLFTLTNLGAPAAFKYFD Cas9 1310 1315 1320 1325 IIHLFTLTNLGAPAAFKYFD Cas9 IIHLFTLTNLGAPAAFKYFD t c a t c c a t c t c t t c a c c c t t a c c a a c c t c g g t g c t c c t g c t g c t t t c a a g t a c t t c g a t a a g t a g g t a g a g a a g t g g g a a t g g t t g g a g c c a c g a g g a c g a c g a a a g t t c a t g a a g c t a t

6720 1330 1335 1340 1345 TTIDRKRYTSTKEVLDATLI Cas9 1330 1335 1340 1345 TTIDRKRYTSTKEVLDATLI Cas9 TTIDRKRYTSTKEVLDATLI c a a c c a t c g a t a g g a a g a g a t a c a c c t c t a c c a a a g a a g t g c t c g a t g c t a c c c t c a t c c g t t g g t a g c t a t c c t t c t c t a t g t g g a g a t g g t t t c t t c a c g a g c t a c g a t g g g a g t a g g

6780 1350 1355 1360 1365 HQSITGLYETRIDLSQLGGD Cas9 1350 1355 1360 1365 HQSITGLYETRIDLSQLGGD Cas9 HQSITGLYETRIDLSQLGGD a t c a g t c t a t c a c t g g a c t c t a c g a g a c t a g g a t c g a t c t c t c a c a g c t c g g t g g t g a t t t a g t c a g a t a g t g a c c t g a g a t g c t c t g a t c c t a g c t a g a g a g t g t c g a g c c a c c a c t a a

BsaHIZraISgrDISalIAatIIAccI 6840 SRAD 130 PKKKRKV * SV40 NLS HSP terminator (in frame with Cas9) SRADPKKKRKV* SRADPKKKRKV* c a a g g g c t g a t c c t a a g a a g a a g a g g a a g g t t t g a c g t c g a c g a t a t g a a g a t g a a g a t g g t t c c c g a c t a g g a t t c t t c t t c t c c t t c c a a a c t g c a g c t g c t a t a c t t c t a c t t c t a c

6900 HSP terminator a a a t a t t t g g t g t g t c a a a t a a a a a g c t t g t g t g c t t a a g t t t g t g t t t t t t t c t t g g c t t t t a t a a a c c a c a c a g t t t a t t t t t c g a a c a c a c g a a t t c a a a c a c a a a a a a a g a a c c g a

6960 HSP terminator t g t t g t g t t a t g a a t t t g t g g c t t t t t c t a a t a t t a a a t g a a t g t a a g a t c a c a t t a t a a a c a a c a c a a t a c t t a a a c a c c g a a a a a g a t t a t a a t t t a c t t a c a t t c t a g t g t a a t a t t

7020 HSP terminator t g a a t a a a c a a a t g t t t c t a t a a t c c a t t g t g a a t g t t t t g t t g g a t c t c t t c t g c a g c a a c t t a t t t g t t t a c a a a g a t a t t a g g t a a c a c t t a c a a a a c a a c c t a g a g a a g a c g t c g t

Eco53kI 7080 HSP terminator t a t a a c t a c t g t a t g t g c t a t g g t a t g g a c t a t g g a a t a t g a t t a a a g a t a a g c c a g a g c a t a t t g a t g a c a t a c a c g a t a c c a t a c c t g a t a c c t t a t a c t a a t t t c t a t t c g g t c t c g

Bsu36IBmgBISacI 7140 t c t g g t g a c g g a c g t g g g c a g g t g t g a t g a a a a g c c t t a g g g t c a g g t g g c a c t t t t c g g a g a c c a c t g c c t g c a c c c g t c c a c a c t a c t t t t c g g a a t c c c a g t c c a c c g t g a a a a g c c

7200 AmpR promoter g g a a a t g t g c g a t g a a c c c c t a t t t g t t t a t t t t t c t a a a t a c a t t c a a a t a t g t a t c c g c c t t t a c a c g c t a c t t g g g g a t a a a c a a a t a a a a a g a t t t a t g t a a g t t t a t a c a t a g g c

7260 AmpR promoter MS c t a a t g a g a c a a t a a c c c t g a t a a a t g c t t c a a t t a t a t t g a a a a a g g a a g a g t a t g a g t g a t t a c t c t g t t a t t g g g a c t a t t t a c g a a g t t a a t a t a a c t t t t t c c t t c t c a t a c t c a

7320 IQHFRVALIPFFAAFCLPVF a t c c a a c a t t t c c g t g t c g c c c t t a t t c c c t t t t t t g c g g c c t t c t g c c t t c c t g t t t t t t a g g t t g t a a a g g c a c a g c g g g a a t a a g g g a a a a a a c g c c g g a a g a c g g a a g g a c a a a a a

7380 AHPETLVKVKDAEDQLGARV g c t c a c c c a g a a a c g c t g g t g a a a g t a a a a g a t g c t g a a g a t c a g t t g g g t g c c c g a g t g c g a g t g g g t c t t t g c g a c c a c t t t c a t t t t c t a c g a c t t c t a g t c a a c c c a c g g g c t c a c

7440 GYIELDLNSGKILESFRPEE g g t t a c a t t g a a c t g g a t c t c a a c a g c g g c a a a a t c t t a g a g a g t t t t c g c c c c g a a g a a c c a a t g t a a c t t g a c c t a g a g t t g t c g c c g t t t t a g a a t c t c t c a a a a g c g g g g c t t c t t

7500 RFPMMSTFKVLLCGAVLSRI c g c t t c c c a a t g a t g a g c a c t t t c a a a g t t c t g c t a t g t g g c g c g g t g t t a t c c c g t a t t g c g a a g g g t t a c t a c t c g t g a a a g t t t c a a g a c g a t a c a c c g c g c c a c a a t a g g g c a t a a

7560 DAGQEQLGRRIHYSQNDLVE g a t g c t g g g c a a g a g c a a c t c g g t c g c c g c a t a c a c t a t t c t c a g a a t g a c t t g g t t g a g c t a c g a c c c g t t c t c g t t g a g c c a g c g g c g t a t g t g a t a a g a g t c t t a c t g a a c c a a c t c

7620 YSPVTEKHLTDGMTVRELCS t a t t c a c c a g t c a c a g a a a a g c a t c t t a c g g a t g g c a t g a c a g t a a g a g a a t t a t g t a g t a t a a g t g g t c a g t g t c t t t t c g t a g a a t g c c t a c c g t a c t g t c a t t c t c t t a a t a c a t c a

7680 AAITMSDNTAANLLLTTIGG g c t g c c a t a a c c a t g a g t g a t a a c a c t g c g g c c a a c t t a c t t c t g a c a a c g a t t g g a g g a c g a c g g t a t t g g t a c t c a c t a t t g t g a c g c c g g t t g a a t g a a g a c t g t t g c t a a c c t c c t

7740 PKELTAFLHNMGDHVTRLDR c c g a a g g a g c t a a c c g c t t t t t t g c a c a a c a t g g g g g a t c a t g t a a c t c g c c t t g a t c g t g g c t t c c t c g a t t g g c g a a a a a a c g t g t t g t a c c c c c t a g t a c a t t g a g c g g a a c t a g c a

7800 WEPELNEAIPNDERDTTMPV t g g g a a c c g g a g c t g a a t g a a g c c a t a c c a a a c g a c g a g c g t g a c a c c a c g a t g c c t g t a a c c c t t g g c c t c g a c t t a c t t c g g t a t g g t t t g c t g c t c g c a c t g t g g t g c t a c g g a c a t

7860 AMATTLRKLLTGELLTLASR g c a a t g g c a a c a a c t c t c c g c a a a c t a t t a a c t g g c g a a c t a c t t a c t c t t g c t t c a c g c c g t t a c c g t t g t t g a g a g g c g t t t g a t a a t t g a c c g c t t g a t g a a t g a g a a c g a a g t g c g

NmeAIII 7920 QQLIDWMEADKVAGPLLRSA c a g c a a c t c a t t g a c t g g a t g g a g g c g g a c a a a g t t g c a g g a c c a c t t c t g c g c t c g g c a g t c g t t g a g t a a c t g a c c t a c c t c c g c c t g t t t c a a c g t c c t g g t g a a g a c g c g a g c c g t

7980 LPAGWFIADKSGAGERGSRG c t t c c g g c t g g c t g g t t t a t t g c t g a t a a a t c t g g a g c c g g t g a g c g t g g g t c t c g c g g t g a a g g c c g a c c g a c c a a a t a a c g a c t a t t t a g a c c t c g g c c a c t c g c a c c c a g a g c g c c a

8040 IIAALGPDGKPSRIVVIYTT a t c a t t g c a g c a c t g g g g c c a g a t g g t a a g c c c t c c c g t a t c g t a g t t a t c t a c a c g a c g t a g t a a c g t c g t g a c c c c g g t c t a c c a t t c g g g a g g g c a t a g c a t c a a t a g a t g t g c t g c

AhdI 8100 GSQATMDERNRQIAEIGASL g g g a g t c a g g c a a c t a t g g a t g a a c g a a a t a g a c a g a t c g c t g a g a t a g g t g c c t c a c t g c c c t c a g t c c g t t g a t a c c t a c t t g c t t t a t c t g t c t a g c g a c t c t a t c c a c g g a g t g a c

8160 IKHW* a t t a a g c a t t g g t a a c t g t c a g a c g a a g g g a a a t a a a t a g t a g c c c g c c t g a t g t g c g g g t a a t t c g t a a c c a t t g a c a g t c t g c t t c c c t t t a t t t a t c a t c g g g c g g a c t a c a c g c c c

RsrII 8220 c t t t t t t t t g g t g g t g g t t a c c a g t g t c g c t t a g c t a c c g t a g g t c c t c t t a g g c g g t c c g a a a a a a a a c c a c c a c c a a t g g t c a c a g c g a a t c g a t g g c a t c c a g g a g a a t c c g c c a g g

3ʹ 5ʹ 8242 g a t t a g c g a c a t a g t c a t c a a t c t a a t c g c t g t a t c a g t a g t t a

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.