Addgene pAAV-CaMKII-ASAP2s - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pAAV-CaMKII-ASAP2s


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PciI (6651) pBR322ori-F (6496 .. 6515) FspI (5540) PvuI (5394) ScaI (5282) Amp-R (5194 .. 5213) pBRforEco (4785 .. 4803) pGEX 3' (4725 .. 4747) pRS-marker (4606 .. 4625) PsiI (4492) F1ori-F (4431 .. 4452) F1ori-R (4221 .. 4240) PluTI (4064) SfoI (4062) NarI (4061) KasI (4060) RsrII (3904) PmlI (3899) BstXI (3837) NsiI (3721) BbvCI (3686) hGH-PA-R (3602 .. 3621) AleI (3460) BglII (3395) PaeR7I - XhoI (3389) MluI (150) EcoNI (555) StuI (587) PflFI - Tth111I (642) BsmI (657) HincII (771) BspQI - SapI (1066) BclI * (1080) PaqCI (1232) PpuMI * (1306) NruI (1330) Acc65I (1457) KpnI (1461) BamHI (1463) AccI (1680) BsaBI * (2028) SgrAI (2204) AgeI (2374) BspEI * (2437) NdeI (2445) EcoRI (2759) EcoRV (2767) HindIII (2771) WPRE-R (2836 .. 2856) AfeI (3384) pAAV-CaMKII-ASAP2s 6656 bp

Sequence View

5ʹ 3ʹ 60 AAV2 ITR AAV2 ITR c c t g c a g g c a g c t g c g c g c t c g c t c g c t c a c t g a g g c c g c c c g g g c a a a g c c c g g g c g t c g g a c g t c c g t c g a c g c g c g a g c g a g c g a g t g a c t c c g g c g g g c c c g t t t c g g g c c c g c a g

120 AAV2 ITR AAV2 ITR g g g c g a c c t t t g g t c g c c c g g c c t c a g t g a g c g a g c g a g c g c g c a g a g a g g g a g t g g c c a c c c g c t g g a a a c c a g c g g g c c g g a g t c a c t c g c t c g c t c g c g c g t c t c t c c c t c a c c g g t

MluI 180 AAV2 ITR CaMKII promoter AAV2 ITR a c t c c a t c a c t a g g g g t t c c t g c g g c c g c a c g c g t t t a a c a t t a t g g c c t t a g g t c a c t t t g a g g t a g t g a t c c c c a a g g a c g c c g g c g t g c g c a a a t t g t a a t a c c g g a a t c c a g t g a a

240 CaMKII promoter c a t c t c c a t g g g g t t c t t c t t c t g a t t t t c t a g a a a a t g a g a t g g g g g t g c a g a g a g c t t g t a g a g g t a c c c c a a g a a g a a g a c t a a a a g a t c t t t t a c t c t a c c c c c a c g t c t c t c g a a

300 CaMKII promoter c c t c a g t g a c c t g c c c a g g g t c a c a t c a g a a a t g t c a g a g c t a g a a c t t g a a c t c a g a t t g g a g t c a c t g g a c g g g t c c c a g t g t a g t c t t t a c a g t c t c g a t c t t g a a c t t g a g t c t a a

360 CaMKII promoter a c t a a t c t t a a a t t c c a t g c c t t g g g g g c a t g c a a g t a c g a t a t a c a g a a g g a g t g a a c t t g a t t a g a a t t t a a g g t a c g g a a c c c c c g t a c g t t c a t g c t a t a t g t c t t c c t c a c t t g a

420 CaMKII promoter c a t t a g g g c a g a t g a c c a a t g a g t t t a g g a a a g a a g a g t c c a g g g c a g g g t a c a t c t a c a g t a a t c c c g t c t a c t g g t t a c t c a a a t c c t t t c t t c t c a g g t c c c g t c c c a t g t a g a t g t

480 CaMKII promoter c c a c c c g c c c a g c c c t g g g t g a g t c c a g c c a c g t t c a c c t c a t t a t a g t t g c c t c t c t c c g g t g g g c g g g t c g g g a c c c a c t c a g g t c g g t g c a a g t g g a g t a a t a t c a a c g g a g a g a g g

540 CaMKII promoter a g t c c t a c c t t g a c g g g a a g c a c a a g c a g a a a c t g g g a c a g g a g c c c c a g g a g a c c a a a t t c a g g a t g g a a c t g c c c t t c g t g t t c g t c t t t g a c c c t g t c c t c g g g g t c c t c t g g t t t a

StuIEcoNI 600 CaMKII promoter c t t c a t g g t c c c t c t g g g a g g a t g g g t g g g g a g a g c t g t g g c a g a g g c c t c a g g a g g g g c g a a g t a c c a g g g a g a c c c t c c t a c c c a c c c c t c t c g a c a c c g t c t c c g g a g t c c t c c c c g

BsmITth111IPflFI 660 CaMKII promoter c c t g c t g c t c a g t g g t g a c a g a t a g g g g t g a g a a a g c a g a c a g a g t c a t t c c g t c a g c a t g g a c g a c g a g t c a c c a c t g t c t a t c c c c a c t c t t t c g t c t g t c t c a g t a a g g c a g t c g t a

720 CaMKII promoter t c t g g g t c t g t t t g g t a c t t c t t c t c a c g c t a a g g t g g c g g t g t g a t a t g c a c a a t g g c t a g a c c c a g a c a a a c c a t g a a g a a g a g t g c g a t t c c a c c g c c a c a c t a t a c g t g t t a c c g a

HincII 780 CaMKII promoter a a a a a g c a g g g a g a g c t g g a a a g a a a c a a g g a c a g a g a c a g a g g c c a a g t c a a c c a g a c c t t t t t c g t c c c t c t c g a c c t t t c t t t g t t c c t g t c t c t g t c t c c g g t t c a g t t g g t c t g g

840 CaMKII promoter a a t t c c c a g a g g a a g c a a a g a a a c c a t t a c a g a g a c t a c a a g g g g g a a g g g a a g g a g a g a t t a a g g g t c t c c t t c g t t t c t t t g g t a a t g t c t c t g a t g t t c c c c c t t c c c t t c c t c t c t

900 CaMKII promoter t g a a t t a g c t t c c c c t g t a a a c c t t a g a a c c c a g c t g t t g c c a g g g c a a c g g g g c a a t a c a c t t a a t c g a a g g g g a c a t t t g g a a t c t t g g g t c g a c a a c g g t c c c g t t g c c c c g t t a t g

960 CaMKII promoter c t g t c t c t t c a g a g g a g a t g a a g t t g c c a g g g t a a c t a c a t c c t g t c t t t c t c a a g g a c c g a c a g a g a a g t c t c c t c t a c t t c a a c g g t c c c a t t g a t g t a g g a c a g a a a g a g t t c c t g g

1020 CaMKII promoter a t c c c a g a a t g t g g c a c c c a c t a g c c g t t a c c a t a g c a a c t g c c t c t t t g c c c c a c t t a a t a g g g t c t t a c a c c g t g g g t g a t c g g c a a t g g t a t c g t t g a c g g a g a a a c g g g g t g a a t t

BspQISapI 1080 CaMKII promoter t c c c a t c c c g t c t g t t a a a a g g g c c c t a t a g t t g g a g g t g g g g g a g g t a g g a a g a g c g a t a g g g t a g g g c a g a c a a t t t t c c c g g g a t a t c a a c c t c c a c c c c c t c c a t c c t t c t c g c t a

BclI* 1140 CaMKII promoter g a t c a c t t g t g g a c t a a g t t t g t t c g c a t c c c c t t c t c c a a c c c c c t c a g t a c a t c a c c c c t a g t g a a c a c c t g a t t c a a a c a a g c g t a g g g g a a g a g g t t g g g g g a g t c a t g t a g t g g g

1200 CaMKII promoter t g g g g g a a c a g g g t c c a c t t g c t c c t g g g c c c a c a c a g t c c t g c a g t a t t g t g t a t a t a a a c c c c c t t g t c c c a g g t g a a c g a g g a c c c g g g t g t g t c a g g a c g t c a t a a c a c a t a t a t t

PaqCI 1260 CaMKII promoter g g c c a g g g c a a a g a g g a g c a g g t t t t a a a g t g a a a g g c a g g c a g g t g t t g g g g a g g c a g t c c g g t c c c g t t t c t c c t c g t c c a a a a t t t c a c t t t c c g t c c g t c c a c a a c c c c t c c g t c a

PpuMI* 1320 CaMKII promoter t a c c g g g g c a a c g g g a a c a g g g c g t t t c g g a g g t g g t t g c c a t g g g g a c c t g g a t g c t g a a t g g c c c c g t t g c c c t t g t c c c g c a a a g c c t c c a c c a a c g g t a c c c c t g g a c c t a c g a c t

NruI 1380 CaMKII promoter c g a a g g c t c g c g a g g c t g t g a g c a g c c a c a g t g c c c t g c t c a g a a g c c c c a a g c t c g t c a g c t t c c g a g c g c t c c g a c a c t c g t c g g t g t c a c g g g a c g a g t c t t c g g g g t t c g a g c a g t

1440 CaMKII promoter g t c a a g c c g g t t c t c c g t t t g c a c t c a g g a g c a c g g g c a g g c g a g t g g c c c c t a g t t c t g c a g t t c g g c c a a g a g g c a a a c g t g a g t c c t c g t g c c c g t c c g c t c a c c g g g g a t c a a g a c

Acc65IKpnIBamHI 1500 CaMKII promoter Kozak sequence METTVRYEQ g g g g c a g c t c t a g a g c g g t a c c g g a t c c g c c a c c a t g g a g a c g a c t g t g a g g t a t g a a c a c c c c g t c g a g a t c t c g c c a t g g c c t a g g c g g t g g t a c c t c t g c t g a c a c t c c a t a c t t g t

1560 GSELTKTSSSPTADEPTIKI g g g g t c a g a g c t c a c t a a a a c t t c g a g c t c t c c a a c a g c a g a t g a g c c c a c g a t a a a g a t c c c c a g t c t c g a g t g a t t t t g a a g c t c g a g a g g t t g t c g t c t a c t c g g g t g c t a t t t c t a

1620 DDGRDEGNEQDSCSNTIRRK t g a t g a t g g t c g t g a t g a g g g t a a t g a a c a a g a c a g c t g t t c c a a t a c c a t t a g g a g a a a a c t a c t a c c a g c a c t a c t c c c a t t a c t t g t t c t g t c g a c a a g g t t a t g g t a a t c c t c t t t

1680 ISPFVMSFGFRVFGVVLIIV a a t t t c c c c g t t t g t g a t g t c a t t t g g a t t c a g a g t a t t t g g a g t t g t g c t t a t c a t t g t t t a a a g g g g c a a a c a c t a c a g t a a a c c t a a g t c t c a t a a a c c t c a a c a c g a a t a g t a a c a

AccI 1740 DIIVVIVDLAISEKKRGIRE a g a c a t c a t a g t g g t g a t t g t g g a t c t g g c c a t c a g t g a g a a g a a a a g a g g c a t t a g a g a t c t g t a g t a t c a c c a c t a a c a c c t a g a c c g g t a g t c a c t c t t c t t t t c t c c g t a a t c t c t

1800 ILEGVSLAIALFFLVDVLMR g a t t c t t g a a g g t g t t t c c c t g g c t a t a g c a c t c t t c t t c c t t g t t g a t g t t c t c a t g a g c t a a g a a c t t c c a c a a a g g g a c c g a t a t c g t g a g a a g a a g g a a c a a c t a c a a g a g t a c t c

1860 VFVEGFKNYFRSKLNTLDAV a g t g t t t g t t g a a g g c t t c a a g a a c t a t t t c c g g t c c a a a c t g a a t a c t t t g g a t g c a g t t c a c a a a c a a c t t c c g a a g t t c t t g a t a a a g g c c a g g t t t g a c t t a t g a a a c c t a c g t c a

1920 IVVGTLLINMTYSFSDLAAF c a t a g t a g t g g g c a c t c t g c t a a t t a a t a t g a c c t a c t c c t t c t c t g a c c t t g c t g c c t t g t a t c a t c a c c c g t g a g a c g a t t a a t t a t a c t g g a t g a g g a a g a g a c t g g a a c g a c g g a a

1980 NSHNVYITADKQKNGIKANF t a a c a g c c a t a a c g t g t a t a t t a c c g c g g a t a a a c a g a a a a a c g g c a t t a a a g c g a a c t t a t t g t c g g t a t t g c a c a t a t a a t g g c g c c t a t t t g t c t t t t t g c c g t a a t t t c g c t t g a a

BsaBI* 2040 TVRHNVEDGSVQLADHYQQN t a c c g t g c g c c a t a a c g t g g a a g a t g g c a g c g t g c a g c t g g c g g a t c a t t a t c a g c a g a a a t g g c a c g c g g t a t t g c a c c t t c t a c c g t c g c a c g t c g a c c g c c t a g t a a t a g t c g t c t t

2100 TPIGDGPVLLPDNHYLSTQT c a c c c c g a t t g g c g a t g g c c c g g t g c t g c t g c c g g a t a a c c a t t a t c t g a g c a c c c a g a c g t g g g g c t a a c c g c t a c c g g g c c a c g a c g a c g g c c t a t t g g t a a t a g a c t c g t g g g t c t g

2160 VLSKDPNEKRDHMVLLEFVT c g t g c t g a g c a a a g a t c c g a a c g a a a a a c g c g a t c a c a t g g t g c t g c t g g a a t t t g t g a c g c a c g a c t c g t t t c t a g g c t t g c t t t t t g c g c t a g t g t a c c a c g a c g a c c t t a a a c a c t g

SgrAI 2220 AAGITHGMDELYGGTGGSAS c g c a g c g g g c a t t a c a c a c g g c a t g g a t g a a c t g t a t g g c g g c a c c g g c g g c a g c g c g a g g c g t c g c c c g t a a t g t g t g c c g t a c c t a c t t g a c a t a c c g c c g t g g c c g c c g t c g c g c t c

2280 QGEELFTGVVPILVELDGDV c c a g g g c g a a g a a c t g t t t a c c g g c g t g g t g c c g a t t c t g g t g g a a c t g g a t g g c g a t g t g g t c c c g c t t c t t g a c a a a t g g c c g c a c c a c g g c t a a g a c c a c c t t g a c c t a c c g c t a c a

2340 NGHKFSVRGEGEGDATIGKL g a a c g g c c a t a a a t t t a g c g t g c g c g g c g a a g g c g a a g g c g a t g c g a c c a t t g g c a a a c t c t t g c c g g t a t t t a a a t c g c a c g c g c c g c t t c c g c t t c c g c t a c g c t g g t a a c c g t t t g a

AgeI 2400 TLKFICTTGKLPVPWPTLVT g a c c c t g a a a t t t a t t t g c a c c a c c g g c a a a c t a c c g g t g c c g t g g c c g a c c c t g g t g a c c t g g g a c t t t a a a t a a a c g t g g t g g c c g t t t g a t g g c c a c g g c a c c g g c t g g g a c c a c t g

NdeIBspEI* 2460 TLTYGVQCFSRYPDHMKRHD c a c c t t a a c c t a t g g c g t g c a g t g c t t t a g c c g c t a t c c g g a t c a t a t g a a a c g c c a t g a g t g g a a t t g g a t a c c g c a c g t c a c g a a a t c g g c g a t a g g c c t a g t a t a c t t t g c g g t a c t

2520 FFKSAMPEGYVQERTISFKD t t t t t t t a a a a g c g c g a t g c c g g a a g g c t a t g t g c a g g a a c g c a c c a t t a g c t t t a a a g a a a a a a a a t t t t c g c g c t a c g g c c t t c c g a t a c a c g t c c t t g c g t g g t a a t c g a a a t t t c t

2580 DGKYKTRAVVKFEGDTLVNR t g a t g g c a a a t a t a a a a c c c g c g c g g t g g t g a a a t t t g a a g g c g a t a c c c t g g t g a a c c g a c t a c c g t t t a t a t t t t g g g c g c g c c a c c a c t t t a a a c t t c c g c t a t g g g a c c a c t t g g c

2640 IELKGTDFKEDGNILGHKLE c a t t g a a c t g a a a g g c a c c g a t t t t a a a g a a g a t g g c a a c a t t c t g g g g c a t a a a c t g g a g t a a c t t g a c t t t c c g t g g c t a a a a t t t c t t c t a c c g t t g t a a g a c c c c g t a t t t g a c c t

2700 YNTDQMPQMVTLLRVLRIVI a t a t a a c a c a g a t c a g a t g c c g c a g a t g g t t a c t c t t c t t c g a g t t c t g a g a a t t g t t a t t a t a t t g t g t c t a g t c t a c g g c g t c t a c c a a t g a g a a g a a g c t c a a g a c t c t t a a c a a t a

EcoRI 2760 LIRIFQLASQKKQLEVVT* c t t a a t a a g a a t a t t t c a g c t g g c t t c a c a g a a g a a a c a a c t t g a a g t g g t a a c c t a a g a g a a t t a t t c t t a t a a a g t c g a c c g a a g t g t c t t c t t t g t t g a a c t t c a c c a t t g g a t t c t

EcoRVHindIII 2820 WPRE a t t c g a t a t c a a g c t t a t c g a t a a t c a a c c t c t g g a t t a c a a a a t t t g t g a a a g a t t g a c t a a g c t a t a g t t c g a a t a g c t a t t a g t t g g a g a c c t a a t g t t t t a a a c a c t t t c t a a c t g

2880 WPRE A C A A C G A G G A A A A T G C G A T A C WPRE-R MPL t g g t a t t c t t a a c t a t g t t g c t c c t t t t a c g c t a t g t g g a t a c g c t g c t t t a a t g c c t t t a c c a t a a g a a t t g a t a c a a c g a g g a a a a t g c g a t a c a c c t a t g c g a c g a a a t t a c g g a a a

2940 WPRE YHAIASRMAFIFSSLYKSWL MLLLPVWLSFSPPCINPG g t a t c a t g c t a t t g c t t c c c g t a t g g c t t t c a t t t t c t c c t c c t t g t a t a a a t c c t g g t t c a t a g t a c g a t a a c g a a g g g c a t a c c g a a a g t a a a a g a g g a g g a a c a t a t t t a g g a c c a a

3000 WPRE LSLYEELWPVVRQRGVVCTV CCLFMRSCGPLSGNVAWCAL g c t g t c t c t t t a t g a g g a g t t g t g g c c c g t t g t c a g g c a a c g t g g c g t g g t g t g c a c t g t c g a c a g a g a a a t a c t c c t c a a c a c c g g g c a a c a g t c c g t t g c a c c g c a c c a c a c g t g a c a

3060 WPRE FADATPTGWGIATTCQLLSG CLLTQPPLVGALPPPVSSFP g t t t g c t g a c g c a a c c c c c a c t g g t t g g g g c a t t g c c a c c a c c t g t c a g c t c c t t t c c g g c a a a c g a c t g c g t t g g g g g t g a c c a a c c c c g t a a c g g t g g t g g a c a g t c g a g g a a a g g c c

3120 WPRE TFAFPLPIATAELIAACLAR GLSLSPSLLPRRNSSPPALP g a c t t t c g c t t t c c c c c t c c c t a t t g c c a c g g c g g a a c t c a t c g c c g c c t g c c t t g c c c g c t g a a a g c g a a a g g g g g a g g g a t a a c g g t g c c g c c t t g a g t a g c g g c g g a c g g a a c g g g c

3180 WPRE * CWTGARLLGTDNSVVLSGKS AAGQGLGCWALTIPWCCRGN c t g c t g g a c a g g g g c t c g g c t g t t g g g c a c t g a c a a t t c c g t g g t g t t g t c g g g g a a a t c g a c g a c c t g t c c c c g a g c c g a c a a c c c g t g a c t g t t a a g g c a c c a c a a c a g c c c c t t t a g

3240 WPRE (in frame with Factor Xa site) RGKRPQEGTNGGPNQAPRGE SSFPWLLACVATWILRGTSF HRPFLGCSPVLPPGFCAGRP a t c g t c c t t t c c t t g g c t g c t c g c c t g t g t t g c c a c c t g g a t t c t g c g c g g g a c g t c c t t t a g c a g g a a a g g a a c c g a c g a g c g g a c a c a a c g g t g g a c c t a a g a c g c g c c c t g c a g g a a

3300 WPRE AVDR 1 RGEI Factor Xa site CYVPSALNPADLPSRGLLPA SATSLRPSIQRTFLPAACCR c t g c t a c g t c c c t t c g g c c c t c a a t c c a g c g g a c c t t c c t t c c c g c g g c c t g c t g c c g g c g a c g a t g c a g g g a a g c c g g g a g t t a g g t c g c c t g g a a g g a a g g g c g c c g g a c g a c g g c c g

3360 WPRE LRPLPRLRLRPQTSRISLWA LCGLFRVFAFALRRVGSPFG t c t g c g g c c t c t t c c g c g t c t t c g c c t t c g c c c t c a g a c g a g t c g g a t c t c c c t t t g g g c a g a c g c c g g a g a a g g c g c a g a a g c g g a a g c g g g a g t c t g c t c a g c c t a g a g g g a a a c c c g

BglIIPaeR7IXhoIAfeI 3420 WPRE hGH poly(A) signal ASPHRYRALLERSTGGIPVT PPPRIDTERCSRDLRVASL* c g c c t c c c c g c a t c g a t a c c g a g c g c t g c t c g a g a g a t c t a c g g g t g g c a t c c c t g t g a c g c g g a g g g g c g t a g c t a t g g c t c g c g a c g a g c t c t c t a g a t g c c c a c c g t a g g g a c a c t g

AleI 3480 hGH poly(A) signal PPQCLSWPWKLPLQCPPALS c c c t c c c c a g t g c c t c t c c t g g c c c t g g a a g t t g c c a c t c c a g t g c c c a c c a g c c t t g t c g g g a g g g g t c a c g g a g a g g a c c g g g a c c t t c a a c g g t g a g g t c a c g g g t g g t c g g a a c a g

3540 hGH poly(A) signal * c t a a t a a a a t t a a g t t g c a t c a t t t t g t c t g a c t a g g t g t c c t t c t a t a a t a t t a t g g g g g a t t a t t t t a a t t c a a c g t a g t a a a a c a g a c t g a t c c a c a g g a a g a t a t t a t a a t a c c c c

3600 hGH poly(A) signal t g g a g g g g g g t g g t a t g g a g c a a g g g g c a a g t t g g g a a g a c a a c c t g t a g g g c c t g c g g g a c c t c c c c c c a c c a t a c c t c g t t c c c c g t t c a a c c c t t c t g t t g g a c a t c c c g g a c g c c c

3660 hGH poly(A) signal A G A T A A C C C T T G G T T C G A C C hGH-PA-R g t c t a t t g g g a a c c a a g c t g g a g t g c a g t g g c a c a a t c t t g g c t c a c t g c a a t c t c c g c c c a g a t a a c c c t t g g t t c g a c c t c a c g t c a c c g t g t t a g a a c c g a g t g a c g t t a g a g g c g g

BbvCI 3720 hGH poly(A) signal t c c t g g g t t c a a g c g a t t c t c c t g c c t c a g c c t c c c g a g t t g t t g g g a t t c c a g g c a t g c a g g a c c c a a g t t c g c t a a g a g g a c g g a g t c g g a g g g c t c a a c a a c c c t a a g g t c c g t a c g

NsiI 3780 hGH poly(A) signal a t g a c c a g g c t c a g c t a a t t t t t g t t t t t t t g g t a g a g a c g g g g t t t c a c c a t a t t g g c c t a c t g g t c c g a g t c g a t t a a a a a c a a a a a a a c c a t c t c t g c c c c a a a g t g g t a t a a c c g g

BstXI 3840 hGH poly(A) signal a g g c t g g t c t c c a a c t c c t a a t c t c a g g t g a t c t a c c c a c c t t g g c c t c c c a a a t t g c t g t c c g a c c a g a g g t t g a g g a t t a g a g t c c a c t a g a t g g g t g g a a c c g g a g g g t t t a a c g a c

PmlI 3900 hGH poly(A) signal g g a t t a c a g g c g t g a a c c a c t g c t c c c t t c c c t g t c c t t c t g a t t t t g t a g g t a a c c a c g c c t a a t g t c c g c a c t t g g t g a c g a g g g a a g g g a c a g g a a g a c t a a a a c a t c c a t t g g t g c

RsrII 3960 AAV2 ITR AAV2 ITR t g c g g a c c g a g c g g c c g c a g g a a c c c c t a g t g a t g g a g t t g g c c a c t c c c t c t c t g c g c g a c g c c t g g c t c g c c g g c g t c c t t g g g g a t c a c t a c c t c a a c c g g t g a g g g a g a g a c g c g c

4020 AAV2 ITR AAV2 ITR c t c g c t c g c t c a c t g a g g c c g g g c g a c c a a a g g t c g c c c g a c g c c c g g g c t t t g c c c g g g g a g c g a g c g a g t g a c t c c g g c c c g c t g g t t t c c a g c g g g c t g c g g g c c c g a a a c g g g c c c

KasINarISfoIPluTI 4080 AAV2 ITR AAV2 ITR c g g c c t c a g t g a g c g a g c g a g c g c g c a g c t g c c t g c a g g g g c g c c t g a t g c g g t a t t t t c g c c g g a g t c a c t c g c t c g c t c g c g c g t c g a c g g a c g t c c c c g c g g a c t a c g c c a t a a a a g

4140 f1 ori t c c t t a c g c a t c t g t g c g g t a t t t c a c a c c g c a t a c g t c a a a g c a a c c a t a g t a c g c g c c a g g a a t g c g t a g a c a c g c c a t a a a g t g t g g c g t a t g c a g t t t c g t t g g t a t c a t g c g c g g

4200 f1 ori c t g t a g c g g c g c a t t a a g c g c g g c g g g t g t g g t g g t t a c g c g c a g c g t g a c c g c t a c a c t g a c a t c g c c g c g t a a t t c g c g c c g c c c a c a c c a c c a a t g c g c g t c g c a c t g g c g a t g t g a

4260 f1 ori G A G G A A A G C G A A A G A A G G G A F1ori-R t g c c a g c g c c c t a g c g c c c g c t c c t t t c g c t t t c t t c c c t t c c t t t c t c g c c a c g t t c g c a c g g t c g c g g g a t c g c g g g c g a g g a a a g c g a a a g a a g g g a a g g a a a g a g c g g t g c a a g c g

4320 f1 ori c g g c t t t c c c c g t c a a g c t c t a a a t c g g g g g c t c c c t t t a g g g t t c c g a t t t a g t g c t t t g c c g a a a g g g g c a g t t c g a g a t t t a g c c c c c g a g g g a a a t c c c a a g g c t a a a t c a c g a a a

4380 f1 ori a c g g c a c c t c g a c c c c a a a a a a c t t g a t t t g g g t g a t g g t t c a c g t a g t g g g c c a t c g c c t g c c g t g g a g c t g g g g t t t t t t g a a c t a a a c c c a c t a c c a a g t g c a t c a c c c g g t a g c g g

4440 f1 ori G T G G A C T C T T F1ori-F c t g a t a g a c g g t t t t t c g c c c t t t g a c g t t g g a g t c c a c g t t c t t t a a t a g t g g a c t c t t g a c t a t c t g c c a a a a a g c g g g a a a c t g c a a c c t c a g g t g c a a g a a a t t a t c a c c t g a g a a

PsiI 4500 f1 ori G T T C C A A A C T G G F1ori-F g t t c c a a a c t g g a a c a a c a c t c a a c c c t a t c t c g g g c t a t t c t t t t g a t t t a t a a g g g a t c a a g g t t t g a c c t t g t t g t g a g t t g g g a t a g a g c c c g a t a a g a a a a c t a a a t a t t c c c t a

4560 f1 ori t t t g c c g a t t t c g g c c t a t t g g t t a a a a a a t g a g c t g a t t t a a c a a a a a t t t a a c g c g a a a a a c g g c t a a a g c c g g a t a a c c a a t t t t t t a c t c g a c t a a a t t g t t t t t a a a t t g c g c t t

4620 f1 ori A T G T T A G A C G A G A C T pRS-marker t t t t a a c a a a a t a t t a a c g t t t a c a a t t t t a t g g t g c a c t c t c a g t a c a a t c t g c t c t g a a a a a t t g t t t t a t a a t t g c a a a t g t t a a a a t a c c a c g t g a g a g t c a t g t t a g a c g a g a c t

4680 A C G G C pRS-marker t g c c g c a t a g t t a a g c c a g c c c c g a c a c c c g c c a a c a c c c g c t g a c g c g c c c t g a c g g g c a c g g c g t a t c a a t t c g g t c g g g g c t g t g g g c g g t t g t g g g c g a c t g c g c g g g a c t g c c c g

4740 C C G G G A G C T G C A T G T G pGEX 3' t t g t c t g c t c c c g g c a t c c g c t t a c a g a c a a g c t g t g a c c g t c t c c g g g a g c t g c a t g t g a a c a g a c g a g g g c c g t a g g c g a a t g t c t g t t c g a c a c t g g c a g a g g c c c t c g a c g t a c a c

4800 T C A G A G G pGEX 3' C G G A G C A C T A T G C G G A pBRforEco t c a g a g g t t t t c a c c g t c a t c a c c g a a a c g c g c g a g a c g a a a g g g c c t c g t g a t a c g c c t a g t c t c c a a a a g t g g c a g t a g t g g c t t t g c g c g c t c t g c t t t c c c g g a g c a c t a t g c g g a

4860 T A A pBRforEco a t t t t t a t a g g t t a a t g t c a t g a t a a t a a t g g t t t c t t a g a c g t c a g g t g g c a c t t t t c g t a a a a a t a t c c a a t t a c a g t a c t a t t a t t a c c a a a g a a t c t g c a g t c c a c c g t g a a a a g c

4920 AmpR promoter g g g a a a t g t g c g c g g a a c c c c t a t t t g t t t a t t t t t c t a a a t a c a t t c a a a t a t g t a t c c c c c t t t a c a c g c g c c t t g g g g a t a a a c a a a t a a a a a g a t t t a t g t a a g t t t a t a c a t a g g

4980 1 signal... MS AmpR promoter AmpR MS g c t c a t g a g a c a a t a a c c c t g a t a a a t g c t t c a a t a a t a t t g a a a a a g g a a g a g t a t g a g c g a g t a c t c t g t t a t t g g g a c t a t t t a c g a a g t t a t t a t a a c t t t t t c c t t c t c a t a c t c

5040 5 10 15 20 signal sequence IQHFRVALIPFFAAFCLPVF AmpR IQHFRVALIPFFAAFCLPVF t a t t c a a c a t t t c c g t g t c g c c c t t a t t c c c t t t t t t g c g g c a t t t t g c c t t c c t g t t t t a t a a g t t g t a a a g g c a c a g c g g g a a t a a g g g a a a a a a c g c c g t a a a a c g g a a g g a c a a a a

5100 signa... A 25 30 35 40 HPETLVKVKDAEDQLGARV AmpR AHPETLVKVKDAEDQLGARV t g c t c a c c c a g a a a c g c t g g t g a a a g t a a a a g a t g c t g a a g a t c a g t t g g g t g c a c g a g t a c g a g t g g g t c t t t g c g a c c a c t t t c a t t t t c t a c g a c t t c t a g t c a a c c c a c g t g c t c a

5160 45 50 55 60 GYIELDLNSGKILESFRPEE AmpR GYIELDLNSGKILESFRPEE g g g t t a c a t c g a a c t g g a t c t c a a c a g c g g t a a g a t c c t t g a g a g t t t t c g c c c c g a a g a c c c a a t g t a g c t t g a c c t a g a g t t g t c g c c a t t c t a g g a a c t c t c a a a a g c g g g g c t t c t

5220 65 70 75 80 RFPMMSTFKVLLCGAVLSRI AmpR C G A T A C A C C G C G C C A T A A T A Amp-R RFPMMSTFKVLLCGAVLSRI a c g t t t t c c a a t g a t g a g c a c t t t t a a a g t t c t g c t a t g t g g c g c g g t a t t a t c c c g t a t t g c a a a a g g t t a c t a c t c g t g a a a a t t t c a a g a c g a t a c a c c g c g c c a t a a t a g g g c a t a

5280 85 90 95 100 DAGQEQLGRRIHYSQNDLVE AmpR DAGQEQLGRRIHYSQNDLVE t g a c g c c g g g c a a g a g c a a c t c g g t c g c c g c a t a c a c t a t t c t c a g a a t g a c t t g g t t g a a c t g c g g c c c g t t c t c g t t g a g c c a g c g g c g t a t g t g a t a a g a g t c t t a c t g a a c c a a c t

ScaI 5340 105 110 115 120 YSPVTEKHLTDGMTVRELCS AmpR YSPVTEKHLTDGMTVRELCS g t a c t c a c c a g t c a c a g a a a a g c a t c t t a c g g a t g g c a t g a c a g t a a g a g a a t t a t g c a g c a t g a g t g g t c a g t g t c t t t t c g t a g a a t g c c t a c c g t a c t g t c a t t c t c t t a a t a c g t c

PvuI 5400 125 130 135 140 AAITMSDNTAANLLLTTIGG AmpR AAITMSDNTAANLLLTTIGG t g c t g c c a t a a c c a t g a g t g a t a a c a c t g c g g c c a a c t t a c t t c t g a c a a c g a t c g g a g g a c g a c g g t a t t g g t a c t c a c t a t t g t g a c g c c g g t t g a a t g a a g a c t g t t g c t a g c c t c c

5460 145 150 155 160 PKELTAFLHNMGDHVTRLDR AmpR PKELTAFLHNMGDHVTRLDR a c c g a a g g a g c t a a c c g c t t t t t t g c a c a a c a t g g g g g a t c a t g t a a c t c g c c t t g a t c g t g g c t t c c t c g a t t g g c g a a a a a a c g t g t t g t a c c c c c t a g t a c a t t g a g c g g a a c t a g c

5520 165 170 175 180 WEPELNEAIPNDERDTTMPV AmpR WEPELNEAIPNDERDTTMPV t t g g g a a c c g g a g c t g a a t g a a g c c a t a c c a a a c g a c g a g c g t g a c a c c a c g a t g c c t g t a a c c c t t g g c c t c g a c t t a c t t c g g t a t g g t t t g c t g c t c g c a c t g t g g t g c t a c g g a c a

FspI 5580 185 190 195 200 AMATTLRKLLTGELLTLASR AmpR AMATTLRKLLTGELLTLASR a g c a a t g g c a a c a a c g t t g c g c a a a c t a t t a a c t g g c g a a c t a c t t a c t c t a g c t t c c c g t c g t t a c c g t t g t t g c a a c g c g t t t g a t a a t t g a c c g c t t g a t g a a t g a g a t c g a a g g g c

5640 205 210 215 220 QQLIDWMEADKVAGPLLRSA AmpR QQLIDWMEADKVAGPLLRSA g c a a c a a t t a a t a g a c t g g a t g g a g g c g g a t a a a g t t g c a g g a c c a c t t c t g c g c t c g g c c g t t g t t a a t t a t c t g a c c t a c c t c c g c c t a t t t c a a c g t c c t g g t g a a g a c g c g a g c c g

5700 225 230 235 240 LPAGWFIADKSGAGERGSRG AmpR LPAGWFIADKSGAGERGSRG c c t t c c g g c t g g c t g g t t t a t t g c t g a t a a a t c t g g a g c c g g t g a g c g t g g g t c t c g c g g g g a a g g c c g a c c g a c c a a a t a a c g a c t a t t t a g a c c t c g g c c a c t c g c a c c c a g a g c g c c

5760 245 250 255 260 IIAALGPDGKPSRIVVIYTT AmpR IIAALGPDGKPSRIVVIYTT t a t c a t t g c a g c a c t g g g g c c a g a t g g t a a g c c c t c c c g t a t c g t a g t t a t c t a c a c g a c a t a g t a a c g t c g t g a c c c c g g t c t a c c a t t c g g g a g g g c a t a g c a t c a a t a g a t g t g c t g

5820 265 270 275 280 GSQATMDERNRQIAEIGASL AmpR GSQATMDERNRQIAEIGASL g g g g a g t c a g g c a a c t a t g g a t g a a c g a a a t a g a c a g a t c g c t g a g a t a g g t g c c t c a c t c c c c t c a g t c c g t t g a t a c c t a c t t g c t t t a t c t g t c t a g c g a c t c t a t c c a c g g a g t g a

5880 285 IKHW* AmpR IKHW* g a t t a a g c a t t g g t a a c t g t c a g a c c a a g t t t a c t c a t a t a t a c t t t a g a t t g a t t t a a a c t a a t t c g t a a c c a t t g a c a g t c t g g t t c a a a t g a g t a t a t a t g a a a t c t a a c t a a a t t t

5940 a c t t c a t t t t t a a t t t a a a a g g a t c t a g g t g a a g a t c c t t t t t g a t a a t c t c a t g a c c a a t g a a g t a a a a a t t a a a t t t t c c t a g a t c c a c t t c t a g g a a a a a c t a t t a g a g t a c t g g t t

6000 a a t c c c t t a a c g t g a g t t t t c g t t c c a c t g a g c g t c a g a c c c c g t a g a a a a g a t c a a a g g t t a g g g a a t t g c a c t c a a a a g c a a g g t g a c t c g c a g t c t g g g g c a t c t t t t c t a g t t t c c

6060 ori a t c t t c t t g a g a t c c t t t t t t t c t g c g c g t a a t c t g c t g c t t g c a a a c a a a a a a a c c a c c t a g a a g a a c t c t a g g a a a a a a a g a c g c g c a t t a g a c g a c g a a c g t t t g t t t t t t t g g t g g

6120 ori g c t a c c a g c g g t g g t t t g t t t g c c g g a t c a a g a g c t a c c a a c t c t t t t t c c g a a g g t a a c c g a t g g t c g c c a c c a a a c a a a c g g c c t a g t t c t c g a t g g t t g a g a a a a a g g c t t c c a t t g

6180 ori t g g c t t c a g c a g a g c g c a g a t a c c a a a t a c t g t c c t t c t a g t g t a g c c g t a g t t a g g c c a a c c g a a g t c g t c t c g c g t c t a t g g t t t a t g a c a g g a a g a t c a c a t c g g c a t c a a t c c g g t

6240 ori c c a c t t c a a g a a c t c t g t a g c a c c g c c t a c a t a c c t c g c t c t g c t a a t c c t g t t a c c a g t g g t g a a g t t c t t g a g a c a t c g t g g c g g a t g t a t g g a g c g a g a c g a t t a g g a c a a t g g t c a

6300 ori g g c t g c t g c c a g t g g c g a t a a g t c g t g t c t t a c c g g g t t g g a c t c a a g a c g a t a g t t a c c c c g a c g a c g g t c a c c g c t a t t c a g c a c a g a a t g g c c c a a c c t g a g t t c t g c t a t c a a t g g

6360 ori g g a t a a g g c g c a g c g g t c g g g c t g a a c g g g g g g t t c g t g c a c a c a g c c c a g c t t g g a g c g c c t a t t c c g c g t c g c c a g c c c g a c t t g c c c c c c a a g c a c g t g t g t c g g g t c g a a c c t c g c

6420 ori a a c g a c c t a c a c c g a a c t g a g a t a c c t a c a g c g t g a g c t a t g a g a a a g c g c c a c g c t t c c t t g c t g g a t g t g g c t t g a c t c t a t g g a t g t c g c a c t c g a t a c t c t t t c g c g g t g c g a a g g

6480 ori c g a a g g g a g a a a g g c g g a c a g g t a t c c g g t a a g c g g c a g g g t c g g a a c a g g a g a g c g c a c g c t t c c c t c t t t c c g c c t g t c c a t a g g c c a t t c g c c g t c c c a g c c t t g t c c t c t c g c g t g

6540 ori G G G A A A C G C C T G G T A T C T T T pBR322ori-F g a g g g a g c t t c c a g g g g g a a a c g c c t g g t a t c t t t a t a g t c c t g t c g g g t t t c g c c a c c t c t c c c t c g a a g g t c c c c c t t t g c g g a c c a t a g a a a t a t c a g g a c a g c c c a a a g c g g t g g a

6600 ori c t g a c t t g a g c g t c g a t t t t t g t g a t g c t c g t c a g g g g g g c g g a g c c t a t g g a a a a a c g c g a c t g a a c t c g c a g c t a a a a a c a c t a c g a g c a g t c c c c c c g c c t c g g a t a c c t t t t t g c g

PciI 3ʹ 5ʹ 6656 c a g c a a c g c g g c c t t t t t a c g g t t c c t g g c c t t t t g c t g g c c t t t t g c t c a c a t g t g t c g t t g c g c c g g a a a a a t g c c a a g g a c c g g a a a a c g a c c g g a a a a c g a g t g t a c a

Restriction Enzymes

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BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

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Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.