Addgene pJT3 Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: pJT3 Sequencing Result


Map View

NheI (11,050) Bsu36I (10,945) BsgI (10,542) PstI - SbfI (9979) SwaI (9915) ApaI (9673) PspOMI (9669) T7 (9636 .. 9655) M13 fwd M13 Forward (9609 .. 9626) M13/pUC Forward (9595 .. 9617) F1ori-R (9363 .. 9382) NaeI (9341) NgoMIV (9339) DraIII (9238) F1ori-F (9151 .. 9172) PluTI (8746) SfoI (8744) NarI (8743) KasI (8742) HpaI (8713) EcoRI (7664) pRS-marker (6698 .. 6717) pGEX 3' (6576 .. 6598) PfoI (6593) pBRforEco (6520 .. 6538) BmgBI (6216) SnaBI (5778) BmtI (11,054) PaeR7I - PspXI - XhoI (335) BlpI (384) BamHI (849) NcoI (2586) SacII (2922) Eco53kI (2929) SacI (2931) T3 (2945 .. 2965) M13 rev M13 Reverse (2984 .. 3000) lac operator M13/pUC Reverse (2997 .. 3019) CAP binding site BspQI - SapI (3209) L4440 (3215 .. 3232) pBR322ori-F (3466 .. 3485) BseYI (3629) PspFI (3633) AhdI (4218) BsaI (4279) ScaI (4698) Amp-R (4768 .. 4787) pJT3 11,185 bp

Sequence View

5ʹ 3ʹ 60 CUP1 promoter t t c t c t t t t a t a t g a t t c t g a c a a t c c a t a t t g c g t t g g t a g t c t t t t t t g c t g g a a c g g a a g a g a a a a t a t a c t a a g a c t g t t a g g t a t a a c g c a a c c a t c a g a a a a a a c g a c c t t g c c

120 CUP1 promoter t t c a g c g g a a a a g a c g c a t c g c t c t t t t t g c t t c t a g a a g a a a t g c c a g c a a a a g a a t c t a a g t c g c c t t t t c t g c g t a g c g a g a a a a a c g a a g a t c t t c t t t a c g g t c g t t t t c t t a g a

180 CUP1 promoter c t t g a c a g t g a c t g a c a g c a a a a a t g t c t t t t t c t a a c t a g t a a c a a g g c t a a g a t a t c a g a a c t g t c a c t g a c t g t c g t t t t t a c a g a a a a a g a t t g a t c a t t g t t c c g a t t c t a t a g t

240 g c c t g a a a t a a a g g g t g g t g a a g t a a t a a t t a a a t c a t c c g t a t a a a c c t a t a c a c a t a t c g g a c t t t a t t t c c c a c c a c t t c a t t a t t a a t t t a g t a g g c a t a t t t g g a t a t g t g t a t a

300 a t g a g g a a a a a t a a t a c a a a a g t g t t t t a a a t a c a g a t a c a t a c a t g a a c a t a t g c a c g t t a c t c c t t t t t a t t a t g t t t t c a c a a a a t t t a t g t c t a t g t a t g t a c t t g t a t a c g t g c a

PaeR7IPspXIXhoI 360 a t a g c g c c c a a a t g t c g g t a a t g g g a t c g g c t t a c t c g a g g a t a g a t a a g c t t a a g g a a t t a t c g c g g g t t t a c a g c c a t t a c c c t a g c c g a a t g a g c t c c t a t c t a t t c g a a t t c c t t a

BlpI 420 t g t c g a c t a a g t c g t a a g g c a t g c t a a g c c g a t c c c a t t a c c g a c a t t t g g g c g c t a t a c a c a g c t g a t t c a g c a t t c c g t a c g a t t c g g c t a g g g t a a t g g c t g t a a a c c c g c g a t a t g

480 g t g c a t a t g t t c a t g t a t g t a t c t g t a t t t a a a a c a c t t t t g t a t t a t t t t t c c t c a t a t c a c g t a t a c a a g t a c a t a c a t a g a c a t a a a t t t t g t g a a a a c a t a a t a a a a a g g a g t a t a

540 a t g t g t a t a g g t t t a t a c g g a t g a t t t a a t t a t t a c t t c a c c a c c c t t t a t t t c a g g c t g t a c a c a t a t c c a a a t a t g c c t a c t a a a t t a a t a a t g a a g t g g t g g g a a a t a a a g t c c g a c

600 CUP1 promoter a t a t c t t a g c c t t g t t a c t a g t t a g a a a a a g a c a t t t t t g c t g t c a g t c a c t g t c a a g a g t a t a g a a t c g g a a c a a t g a t c a a t c t t t t t c t g t a a a a a c g a c a g t c a g t g a c a g t t c t c

660 CUP1 promoter a t t c t t t t g c t g g c a t t t c t t c t a g a a g c a a a a a g a g c g a t g c g t c t t t t c c g c t g a a c c t a a g a a a a c g a c c g t a a a g a a g a t c t t c g t t t t t c t c g c t a c g c a g a a a a g g c g a c t t g g

720 CUP1 promoter g t t c c a g c a a a a a a g a c t a c c a a c g c a a t a t g g a t t g t c a g a a t c a t a t a a a a g a g a a g c c a a g g t c g t t t t t t c t g a t g g t t g c g t t a t a c c t a a c a g t c t t a g t a t a t t t t c t c t t c g

780 CUP1 promoter a a a t a a c t c c t t g t c t t g t a t c a a t t g c a t t a t a a t a t c t t c t t g t t a g t g c a a t a t c a t t t t a t t g a g g a a c a g a a c a t a g t t a a c g t a a t a t t a t a g a a g a a c a a t c a c g t t a t a g t a

840 CUP1 promoter a t a g a a g t c a t c g a a a t a g a t a t t a a g a a a a a c a a a c t g t a c a a t c a a t c a a t c a a t c a t t a t c t t c a g t a g c t t t a t c t a t a a t t c t t t t t g t t t g a c a t g t t a g t t a g t t a g t t a g t a

BamHI 900 MATAAGPIATNNSP c a c a t a a a g g a t c c a a a a t g g c t a c c g c a g c a g g t c c t a t c g c a a c t a a c a a c t c c c c a c g t g t a t t t c c t a g g t t t t a c c g a t g g c g t c g t c c a g g a t a g c g t t g a t t g t t g a g g g g t g

960 QNSNAYRTPIAPSVPITHKW a a a a c t c c a a c g c t t a c a g a a c t c c a a t c g c t c c t t c c g t a c c a a t t a c t c a t a a a t g g t t t t t g a g g t t g c g a a t g t c t t g a g g t t a g c g a g g a a g g c a t g g t t a a t g a g t a t t t a c c a

1020 SIAEDLTCISNPSKHNNPQT c t a t a g c t g a a g a t t t g a c a t g t a t t t c c a a t c c t a g t a a g c a c a a t a a c c c t c a a a c t g g a t a t c g a c t t c t a a a c t g t a c a t a a a g g t t a g g a t c a t t c g t g t t a t t g g g a g t t t g a c

1080 GYRSFSDELYVKYEEKLEDV g t t a c a g a t c a t t t t c t g a c g a a t t a t a c g t t a a g t a c g a a g a a a a g t t g g a a g a t g t t a c a a t g t c t a g t a a a a g a c t g c t t a a t a t g c a a t t c a t g c t t c t t t t c a a c c t t c t a c a a t

1140 RKALREVEENPLEGLVMIDA g a a a a g c a t t a a g a g a a g t t g a a g a a a a c c c t t t g g a a g g t t t a g t t a t g a t a g a c g c t t c t t t t c g t a a t t c t c t t c a a c t t c t t t t g g g a a a c c t t c c a a a t c a a t a c t a t c t g c g a a

1200 LQRLGIDYHFRGEIGAFLQK t g c a a a g a t t g g g t a t c g a t t a c c a t t t c a g a g g t g a a a t t g g t g c a t t c t t g c a a a a g c a c g t t t c t a a c c c a t a g c t a a t g g t a a a g t c t c c a c t t t a a c c a c g t a a g a a c g t t t t c g

1260 QQIISSTPDGYPEHGLYEVS a a c a a a t c a t a t c t t c a a c t c c a g a t g g t t a c c c t g a a c a t g g t t t g t a c g a a g t t t c a a t t g t t t a g t a t a g a a g t t g a g g t c t a c c a a t g g g a c t t g t a c c a a a c a t g c t t c a a a g t t

1320 TLFRFLRQEGHNVTADVFNN c a t t g t t t a g a t t c t t a a g a c a a g a a g g t c a c a a t g t t a c c g c t g a c g t c t t t a a t a a c t g t a a c a a a t c t a a g a a t t c t g t t c t t c c a g t g t t a c a a t g g c g a c t g c a g a a a t t a t t g a

1380 FKDKEGRFRSELSTDIRGLM t c a a g g a t a a g g a a g g t a g a t t c a g a t c a g a a t t g t c a a c a g a t a t t a g a g g t t t g a t g t a g t t c c t a t t c c t t c c a t c t a a g t c t a g t c t t a a c a g t t g t c t a t a a t c t c c a a a c t a c a

1440 SLYEASQLRIEGEDILDQAA c c t t a t a c g a a g c a a g t c a a t t g a g a a t a g a a g g t g a a g a c a t c t t a g a t c a a g c t g c t g g g a a t a t g c t t c g t t c a g t t a a c t c t t a t c t t c c a c t t c t g t a g a a t c t a g t t c g a c g a c

1500 DFSSQLLGRWTKDPNHHEAR a t t t c t c c a g t c a a t t g t t a g g t a g a t g g a c a a a a g a t c c t a a t c a t c a c g a a g c c a g a t t a a a g a g g t c a g t t a a c a a t c c a t c t a c c t g t t t t c t a g g a t t a g t a g t g c t t c g g t c t a

1560 LVSNTLTHPYHKSLATFMGQ t g g t t t c t a a c a c t t t a a c a c a t c c a t a c c a c a a g t c a t t g g c t a c c t t t a t g g g t c a a a a c c a a a g a t t g t g a a a t t g t g t a g g t a t g g t g t t c a g t a a c c g a t g g a a a t a c c c a g t t t

1620 KLSYMNCKGPNWDGVDNLQE a a t t g t c c t a c a t g a a c t g c a a g g g t c c a a a c t g g g a c g g t g t c g a t a a t t t g c a a g a a t t t a a c a g g a t g t a c t t g a c g t t c c c a g g t t t g a c c c t g c c a c a g c t a t t a a a c g t t c t t a

1680 LAKMDLTIVQSIHQKEVFQV t a g c t a a g a t g g a t t t g a c t a t c g t a c a a a g t a t c c a t c a a a a g g a a g t a t t c c a a g t t t a t c g a t t c t a c c t a a a c t g a t a g c a t g t t t c a t a g g t a g t t t t c c t t c a t a a g g t t c a a a

1740 SQWWKDTGLANELKLARNQP c t c a a t g g t g g a a g g a t a c a g g t t t a g c c a a t g a a t t g a a a t t g g c t a g a a a c c a a c c a t g a g t t a c c a c c t t c c t a t g t c c a a a t c g g t t a c t t a a c t t t a a c c g a t c t t t g g t t g g t a

1800 LKWYMWPMAALTDPRFSEER t g a a g t g g t a t a t g t g g c c t a t g g c c g c t t t a a c c g a t c c a a g a t t c a g t g a a g a a a g a g a c t t c a c c a t a t a c a c c g g a t a c c g g c g a a a t t g g c t a g g t t c t a a g t c a c t t c t t t c t c

1860 VELTKPISFIYIIDDIFDVY t t g a a t t a a c t a a g c c t a t t t c t t t t a t a t a c a t c a t a g a t g a c a t c t t c g a c g t c t a t g a a c t t a a t t g a t t c g g a t a a a g a a a a t a t a t g t a g t a t c t a c t g t a g a a g c t g c a g a t a c

1920 GTIEELTLFTDAVNRWELSA g t a c c a t t g a a g a a t t g a c c t t g t t t a c t g a t g c a g t t a a t a g a t g g g a a t t g t c t g c c g c a t g g t a a c t t c t t a a c t g g a a c a a a t g a c t a c g t c a a t t a t c t a c c c t t a a c a g a c g g c

1980 VEQLPDYMKVCFKALYDVTN t c g a a c a a t t a c c a g a c t a c a t g a a a g t a t g t t t c a a g g c t t t g t a c g a t g t t a c c a a c g a g c t t g t t a a t g g t c t g a t g t a c t t t c a t a c a a a g t t c c g a a a c a t g c t a c a a t g g t t g c

2040 EIAYKIYKKHGQNPIDSLQK a a a t c g c a t a c a a a a t c t a t a a a a a g c a t g g t c a a a a c c c t a t t g a t t c c t t g c a a a a g a t t t a g c g t a t g t t t t a g a t a t t t t t c g t a c c a g t t t t g g g a t a a c t a a g g a a c g t t t t c t

2100 TWASLCNAFLVEAKWFASGH c t t g g g c t a g t t t g t g c a a t g c a t t t t t a g t t g a a g c a a a g t g g t t c g c c t c t g g t c a c t g a a c c c g a t c a a a c a c g t t a c g t a a a a a t c a a c t t c g t t t c a c c a a g c g g a g a c c a g t g a

2160 LPNAEEYLKNGIISSGVHVV t g c c a a a t g c a g a a g a a t a c t t a a a g a a c g g t a t c a t c t c t t c a g g t g t t c a t g t t g t c t a c g g t t t a c g t c t t c t t a t g a a t t t c t t g c c a t a g t a g a g a a g t c c a c a a g t a c a a c a g a

2220 LAHMFFLLGDGITQESVDLV t g g c c c a c a t g t t t t t c t t g t t a g g t g a c g g t a t t a c a c a a g a a t c a g t t g a t t t g g t a g a c c g g g t g t a c a a a a a g a a c a a t c c a c t g c c a t a a t g t g t t c t t a g t c a a c t a a a c c a t c

2280 DDYPGISTSIATILRLSDDL a t g a c t a t c c a g g t a t t t c c a c a a g t a t c g c a a c c a t t t t g a g a t t a t c t g a t g a c t t g g t a c t g a t a g g t c c a t a a a g g t g t t c a t a g c g t t g g t a a a a c t c t a a t a g a c t a c t g a a c c

2340 GSAKDEDQDGYDGSYIECYM g t t c a g c c a a a g a t g a a g a c c a a g a t g g t t a t g a t g g t t c t t a c a t c g a a t g t t a c a t g a c a a g t c g g t t t c t a c t t c t g g t t c t a c c a a t a c t a c c a a g a a t g t a g c t t a c a a t g t a c t

2400 KEHKGSSVDSAREEVIRMIS a g g a a c a t a a g g g t t c c a g t g t c g a t t c a g c c a g a g a a g a a g t a a t a a g a a t g a t c t c c g t c c t t g t a t t c c c a a g g t c a c a g c t a a g t c g g t c t c t t c t t c a t t a t t c t t a c t a g a g g c

2460 EAWKCLNKECLSPNPFSESF a a g c a t g g a a a t g t t t g a a t a a g g a a t g c t t a t c a c c a a a c c c t t t t t c t g a a t c a t t c a t t c g t a c c t t t a c a a a c t t a t t c c t t a c g a a t a g t g g t t t g g g a a a a a g a c t t a g t a a g t

2520 RIGSLNMARMIPMMYSYDDN g a a t a g g t t c c t t g a a t a t g g c t a g a a t g a t c c c t a t g a t g t a c t c t t a c g a t g a c a a c c c t t a t c c a a g g a a c t t a t a c c g a t c t t a c t a g g g a t a c t a c a t g a g a a t g c t a c t g t t g g

2580 HNLPILEEHMKAMIYNTSL* a t a a c t t g c c a a t t t t a g a a g a a c a c a t g a a g g c a a t g a t c t a t a a c a c a t c t t t a t a a t t a t t g a a c g g t t a a a a t c t t c t t g t g t a c t t c c g t t a c t a g a t a t t g t g t a g a a a t a t t a

NcoI 2640 a g t g a c c a t g g c g g a t t g a g a g c a a a t c g t t a a g t t c a g g t c a a g t a a a a a t t g a t t t c g t c a c t g g t a c c g c c t a a c t c t c g t t t a g c a a t t c a a g t c c a g t t c a t t t t t a a c t a a a g c

2700 a a a a c t a a t t t c t c t t a t a c a a t c c t t t g a t t g g a c c g t c a t c c t t t c g a a t a t a a g a t t t t t t g a t t a a a g a g a a t a t g t t a g g a a a c t a a c c t g g c a g t a g g a a a g c t t a t a t t c t a a

2760 t t g t t a a g a a t a t t t t a g a c a g a g a t c t a c t t t a t a t t t a a t a t c t a g a t a t t a c a t a a t a a c a a t t c t t a t a a a a t c t g t c t c t a g a t g a a a t a t a a a t t a t a g a t c t a t a a t g t a t t a

2820 t t c c t c t c t a a t a a a a t a t c a t t a a t a a a a t a a a a a t g a a g c g a t t t g a t t t t g t g t t g t a a g g a g a g a t t a t t t t a t a g t a a t t a t t t t a t t t t t a c t t c g c t a a a c t a a a a c a c a a c a

2880 c a a c t t a g t t t g c c g c t a t g c c t c t t g g g t a a t g c t a t t a t t g a a t c g a a g g g c t t t a t t g t t g a a t c a a a c g g c g a t a c g g a g a a c c c a t t a c g a t a a t a a c t t a g c t t c c c g a a a t a a

SacIIEco53kISacI 2940 a t a t t a c c c t t t a g c t t a t t c t g a g g t t t c t g t g g c g t c c g c g g t g g a g c t c c a g c t t t t t a t a a t g g g a a a t c g a a t a a g a c t c c a a a g a c a c c g c a g g c g c c a c c t c g a g g t c g a a a a

3000 T3 promoter M13 rev G G A A A T C A C T C C C A A T T A A C G T3 C A G T A T C G A C A A A G G A C M13 Reverse G G A C M13/pUC Reverse g t t c c c t t t a g t g a g g g t t a a t t g c g c g c t t g g c g t a a t c a t g g t c a t a g c t g t t t c c t g c a a g g g a a a t c a c t c c c a a t t a a c g c g c g a a c c g c a t t a g t a c c a g t a t c g a c a a a g g a c

3060 -10 -35 lac operator lac promoter A C A C T T T A A C A A T A G G C G A M13/pUC Reverse t g t g a a a t t g t t a t c c g c t c a c a a t t c c a c a c a a c a t a c g a g c c g g a a g c a t a a a g t g t a a c a c t t t a a c a a t a g g c g a g t g t t a a g g t g t g t t g t a t g c t c g g c c t t c g t a t t t c a c a t

3120 lac promoter CAP binding site a a g c c t g g g g t g c c t a a t g a g t g a g c t a a c t c a c a t t a a t t g c g t t g c g c t c a c t g c c c g t t c g g a c c c c a c g g a t t a c t c a c t c g a t t g a g t g t a a t t a a c g c a a c g c g a g t g a c g g g c

3180 c t t t c c a g t c g g g a a a c c t g t c g t g c c a g c t g c a t t a a t g a a t c g g c c a a c g c g c g g g g a g a a a g g t c a g c c c t t t g g a c a g c a c g g t c g a c g t a a t t a c t t a g c c g g t t g c g c g c c c c t

BspQISapI 3240 G A G C G A G T G A C T G A G C G A L4440 g a g g c g g t t t g c g t a t t g g g c g c t c t t c c g c t t c c t c g c t c a c t g a c t c g c t g c g c t c g g c t c c g c c a a a c g c a t a a c c c g c g a g a a g g c g a a g g a g c g a g t g a c t g a g c g a c g c g a g c c

3300 t c g t t c g g c t g c g g c g a g c g g t a t c a g c t c a c t c a a a g g c g g t a a t a c g g t t a t c c a c a g a g c a a g c c g a c g c c g c t c g c c a t a g t c g a g t g a g t t t c c g c c a t t a t g c c a a t a g g t g t c

3360 a a t c a g g g g a t a a c g c a g g a a a g a a c a t g t g a g c a a a a g g c c a g c a a a a g g c c a g g a a c c t t a g t c c c c t a t t g c g t c c t t t c t t g t a c a c t c g t t t t c c g g t c g t t t t c c g g t c c t t g g

3420 ori g t a a a a a g g c c g c g t t g c t g g c g t t t t t c c a t a g g c t c c g c c c c c c t g a c g a g c a t c a c a c a t t t t t c c g g c g c a a c g a c c g c a a a a a g g t a t c c g a g g c g g g g g g a c t g c t c g t a g t g t

3480 ori T T T C T A T G G T C C G C A pBR322ori-F a a a a t c g a c g c t c a a g t c a g a g g t g g c g a a a c c c g a c a g g a c t a t a a a g a t a c c a g g c g t t t t t a g c t g c g a g t t c a g t c t c c a c c g c t t t g g g c t g t c c t g a t a t t t c t a t g g t c c g c a

3540 ori A A G G G pBR322ori-F t t c c c c c t g g a a g c t c c c t c g t g c g c t c t c c t g t t c c g a c c c t g c c g c t t a c c g g a t a c c a a g g g g g a c c t t c g a g g g a g c a c g c g a g a g g a c a a g g c t g g g a c g g c g a a t g g c c t a t g g

3600 ori t g t c c g c c t t t c t c c c t t c g g g a a g c g t g g c g c t t t c t c a t a g c t c a c g c t g t a g g t a t c a c a g g c g g a a a g a g g g a a g c c c t t c g c a c c g c g a a a g a g t a t c g a g t g c g a c a t c c a t a g

BseYIPspFI 3660 ori t c a g t t c g g t g t a g g t c g t t c g c t c c a a g c t g g g c t g t g t g c a c g a a c c c c c c g t t c a g c a g t c a a g c c a c a t c c a g c a a g c g a g g t t c g a c c c g a c a c a c g t g c t t g g g g g g c a a g t c g

3720 ori c c g a c c g c t g c g c c t t a t c c g g t a a c t a t c g t c t t g a g t c c a a c c c g g t a a g a c a c g a c t g g c t g g c g a c g c g g a a t a g g c c a t t g a t a g c a g a a c t c a g g t t g g g c c a t t c t g t g c t g a

3780 ori t a t c g c c a c t g g c a g c a g c c a c t g g t a a c a g g a t t a g c a g a g c g a g g t a t g t a g g c g g t g a t a g c g g t g a c c g t c g t c g g t g a c c a t t g t c c t a a t c g t c t c g c t c c a t a c a t c c g c c a c

3840 ori c t a c a g a g t t c t t g a a g t g g t g g c c t a a c t a c g g c t a c a c t a g a a g a a c a g t a t t t g g t a g a t g t c t c a a g a a c t t c a c c a c c g g a t t g a t g c c g a t g t g a t c t t c t t g t c a t a a a c c a t

3900 ori t c t g c g c t c t g c t g a a g c c a g t t a c c t t c g g a a a a a g a g t t g g t a g c t c t t g a t c c g g c a a g a c g c g a g a c g a c t t c g g t c a a t g g a a g c c t t t t t c t c a a c c a t c g a g a a c t a g g c c g t

3960 ori a a c a a a c c a c c g c t g g t a g c g g t g g t t t t t t t g t t t g c a a g c a g c a g a t t a c g c g c a g a a t t g t t t g g t g g c g a c c a t c g c c a c c a a a a a a a c a a a c g t t c g t c g t c t a a t g c g c g t c t t

4020 ori a a a a a g g a t c t c a a g a a g a t c c t t t g a t c t t t t c t a c g g g g t c t g a c g c t c a g t g g a a c g t t t t t c c t a g a g t t c t t c t a g g a a a c t a g a a a a g a t g c c c c a g a c t g c g a g t c a c c t t g c

4080 a a a a c t c a c g t t a a g g g a t t t t g g t c a t g a g a t t a t c a a a a a g g a t c t t c a c c t a g a t c c t t t t g a g t g c a a t t c c c t a a a a c c a g t a c t c t a a t a g t t t t t c c t a g a a g t g g a t c t a g g

4140 t t t t a a a t t a a a a a t g a a g t t t t a a a t c a a t c t a a a g t a t a t a t g a g t a a a c t t g g t c t g a a a a t t t a a t t t t t a c t t c a a a a t t t a g t t a g a t t t c a t a t a t a c t c a t t t g a a c c a g a c

4200 285 280 275 270 *WHKILSAGIEAIQRNRED AmpR *WHKILSAGIEAIQRNRED a c a g t t a c c a a t g c t t a a t c a g t g a g g c a c c t a t c t c a g c g a t c t g t c t a t t t c g t t c a t t g t c a a t g g t t a c g a a t t a g t c a c t c c g t g g a t a g a g t c g c t a g a c a g a t a a a g c a a g t a

AhdI 4260 265 260 255 250 MTAQSGTTYIVVIRSPKGDP AmpR MTAQSGTTYIVVIRSPKGDP c c a t a g t t g c c t g a c t c c c c g t c g t g t a g a t a a c t a c g a t a c g g g a g g g c t t a c c a t c t g g g t a t c a a c g g a c t g a g g g g c a g c a c a t c t a t t g a t g c t a t g c c c t c c c g a a t g g t a g a c

BsaI 4320 245 240 235 230 GLAAIIGRSGREGAGSKDAI AmpR GLAAIIGRSGREGAGSKDAI g c c c c a g t g c t g c a a t g a t a c c g c g a g a c c c a c g c t c a c c g g c t c c a g a t t t a t c a g c a a c g g g g t c a c g a c g t t a c t a t g g c g c t c t g g g t g c g a g t g g c c g a g g t c t a a a t a g t c g t t

4380 225 220 215 210 FWGAPLASRLLPGAVKDAEM AmpR FWGAPLASRLLPGAVKDAEM t a a a c c a g c c a g c c g g a a g g g c c g a g c g c a g a a g t g g t c c t g c a a c t t t a t c c g c c t c c a a t t t g g t c g g t c g g c c t t c c c g g c t c g c g t c t t c a c c a g g a c g t t g a a a t a g g c g g a g g t

4440 205 200 195 190 WDILQQRSALTLLEGTLLKR AmpR WDILQQRSALTLLEGTLLKR t c c a g t c t a t t a a t t g t t g c c g g g a a g c t a g a g t a a g t a g t t c g c c a g t t a a t a g t t t g c a g g t c a g a t a a t t a a c a a c g g c c c t t c g a t c t c a t t c a t c a a g c g g t c a a t t a t c a a a c g

4500 185 180 175 170 LTTAMAVPMTTDREDNPIAE AmpR LTTAMAVPMTTDREDNPIAE g c a a c g t t g t t g c c a t t g c t a c a g g c a t c g t g g t g t c a c g c t c g t c g t t t g g t a t g g c t t c g t t g c a a c a a c g g t a a c g a t g t c c g t a g c a c c a c a g t g c g a g c a g c a a a c c a t a c c g a a

4560 165 160 155 150 NLEPEWRDLRTVHDGMNHLF AmpR NLEPEWRDLRTVHDGMNHLF c a t t c a g c t c c g g t t c c c a a c g a t c a a g g c g a g t t a c a t g a t c c c c c a t g t t g t g c a a a a g t a a g t c g a g g c c a a g g g t t g c t a g t t c c g c t c a a t g t a c t a g g g g g t a c a a c a c g t t t t

4620 145 140 135 130 ATLEKPGGITTLLLNAATND AmpR ATLEKPGGITTLLLNAATND a a g c g g t t a g c t c c t t c g g t c c t c c g a t c g t t g t c a g a a g t a a g t t g g c c g c a g t g t t a t t t c g c c a a t c g a g g a a g c c a g g a g g c t a g c a a c a g t c t t c a t t c a a c c g g c g t c a c a a t a

4680 125 120 115 110 SMTIAASCLERVTMGDTLHK AmpR SMTIAASCLERVTMGDTLHK c a c t c a t g g t t a t g g c a g c a c t g c a t a a t t c t c t t a c t g t c a t g c c a t c c g t a a g a t g c t g t g a g t a c c a a t a c c g t c g t g a c g t a t t a a g a g a a t g a c a g t a c g g t a g g c a t t c t a c g a

ScaI 4740 105 100 95 90 ETVPSYEVLDNQSYHIRRGL AmpR ETVPSYEVLDNQSYHIRRGL t t t c t g t g a c t g g t g a g t a c t c a a c c a a g t c a t t c t g a g a a t a g t g t a t g c g g c g a c c g a a a a g a c a c t g a c c a c t c a t g a g t t g g t t c a g t a a g a c t c t t a t c a c a t a c g c c g c t g g c t

4800 85 80 75 70 QEQGADIRSLVAGCLLVKFT AmpR A T A A T A C C G C G C C A C A T A G C Amp-R QEQGADIRSLVAGCLLVKFT g t t g c t c t t g c c c g g c g t c a a t a c g g g a t a a t a c c g c g c c a c a t a g c a g a a c t t t a a a a g c a a c g a g a a c g g g c c g c a g t t a t g c c c t a t t a t g g c g c g g t g t a t c g t c t t g a a a t t t t c

4860 65 60 55 50 SMMPFREEPRFSELIKGSNL AmpR SMMPFREEPRFSELIKGSNL t g c t c a t c a t t g g a a a a c g t t c t t c g g g g c g a a a a c t c t c a a g g a t c t t a c c g c t g t t g a a c g a g t a g t a a c c t t t t g c a a g a a g c c c c g c t t t t g a g a g t t c c t a g a a t g g c g a c a a c t

4920 45 40 35 30 DLEIYGVRAGLQDEADKVKV AmpR DLEIYGVRAGLQDEADKVKV g a t c c a g t t c g a t g t a a c c c a c t c g t g c a c c c a a c t g a t c t t c a g c a t c t t t t a c t t t c a c t a g g t c a a g c t a c a t t g g g t g a g c a c g t g g g t t g a c t a g a a g t c g t a g a a a a t g a a a g t

4980 25 LTEPH 20 15 10 signal sequence AFVPLCFAAFFPILA AmpR LTEPHAFVPLCFAAFFPILA c c a g c g t t t c t g g g t g a g c a a a a a c a g g a a g g c a a a a t g c c g c a a a a a a g g g a a t a a g g g g g t c g c a a a g a c c c a c t c g t t t t t g t c c t t c c g t t t t a c g g c g t t t t t t c c c t t a t t c c c

5040 5 1 signal sequence VRFHQISM AmpR AmpR promoter VRFHQISM c g a c a c g g a a a t g t t g a a t a c t c a t a c t c t t c c t t t t t c a a t a t t a t t g a a g c a t t t a t c g c t g t g c c t t t a c a a c t t a t g a g t a t g a g a a g g a a a a a g t t a t a a t a a c t t c g t a a a t a g

5100 AmpR promoter a g g g t t a t t g t c t c a t g a g c g g a t a c a t a t t t g a a t g t a t t t a g a a a a a t a a a c a a a t a g t c c c a a t a a c a g a g t a c t c g c c t a t g t a t a a a c t t a c a t a a a t c t t t t t a t t t g t t t a t c

5160 AmpR promoter 2μ ori g g g t t c c g c g c a c a t t t c c c c g a a a a g t g c c a c c t g a a c g a a g c a t c t g t g c t t c a t t t t c c c a a g g c g c g t g t a a a g g g g c t t t t c a c g g t g g a c t t g c t t c g t a g a c a c g a a g t a a a a

5220 2μ ori g t a g a a c a a a a a t g c a a c g c g a g a g c g c t a a t t t t t c a a a c a a a g a a t c t g a g c t g c a t t c a t c t t g t t t t t a c g t t g c g c t c t c g c g a t t a a a a a g t t t g t t t c t t a g a c t c g a c g t a a

5280 2μ ori t t t a c a g a a c a g a a a t g c a a c g c g a a a g c g c t a t t t t a c c a a c g a a g a a t c t g t g c t t c a a a a t g t c t t g t c t t t a c g t t g c g c t t t c g c g a t a a a a t g g t t g c t t c t t a g a c a c g a a g t

5340 2μ ori t t t t t g t a a a a c a a a a a t g c a a c g c g a g a g c g c t a a t t t t t c a a a c a a a g a a t c t g a g c t a a a a a c a t t t t g t t t t t a c g t t g c g c t c t c g c g a t t a a a a a g t t t g t t t c t t a g a c t c g a

5400 2μ ori g c a t t t t t a c a g a a c a g a a a t g c a a c g c g a g a g c g c t a t t t t a c c a a c a a a g a a t c t a t a c g t a a a a a t g t c t t g t c t t t a c g t t g c g c t c t c g c g a t a a a a t g g t t g t t t c t t a g a t a t

5460 2μ ori c t t c t t t t t t g t t c t a c a a a a a t g c a t c c c g a g a g c g c t a t t t t t c t a a c a a a g c a t c t t g a a g a a a a a a c a a g a t g t t t t t a c g t a g g g c t c t c g c g a t a a a a a g a t t g t t t c g t a g a a

5520 2μ ori a g a t t a c t t t t t t t c t c c t t t g t g c g c t c t a t a a t g c a g t c t c t t g a t a a c t t t t t g c a c t c t a a t g a a a a a a a g a g g a a a c a c g c g a g a t a t t a c g t c a g a g a a c t a t t g a a a a a c g t g

5580 2μ ori t g t a g g t c c g t t a a g g t t a g a a g a a g g c t a c t t t g g t g t c t a t t t t c t c t t c c a t a a a a a a c a t c c a g g c a a t t c c a a t c t t c t t c c g a t g a a a c c a c a g a t a a a a g a g a a g g t a t t t t t

5640 2μ ori a a g c c t g a c t c c a c t t c c c g c g t t t a c t g a t t a c t a g c g a a g c t g c g g g t g c a t t t t t t c t t c g g a c t g a g g t g a a g g g c g c a a a t g a c t a a t g a t c g c t t c g a c g c c c a c g t a a a a a a g

5700 2μ ori a a g a t a a a g g c a t c c c c g a t t a t a t t c t a t a c c g a t g t g g a t t g c g c a t a c t t t g t g a a c t t c t a t t t c c g t a g g g g c t a a t a t a a g a t a t g g c t a c a c c t a a c g c g t a t g a a a c a c t t g

5760 2μ ori a g a a a g t g a t a g c g t t g a t g a t t c t t c a t t g g t c a g a a a a t t a t g a a c g g t t t c t t c t a t t c t t t c a c t a t c g c a a c t a c t a a g a a g t a a c c a g t c t t t t a a t a c t t g c c a a a g a a g a t a

SnaBI 5820 2μ ori t t t g t c t c t a t a t a c t a c g t a t a g g a a a t g t t t a c a t t t t c g t a t t g t t t t c g a t t c a c t a a a c a g a g a t a t a t g a t g c a t a t c c t t t a c a a a t g t a a a a g c a t a a c a a a a g c t a a g t g a

5880 2μ ori c t a t g a a t a g t t c t t a c t a c a a t t t t t t t g t c t a a a g a g t a a t a c t a g a g a t a a a c a t a a g a t a c t t a t c a a g a a t g a t g t t a a a a a a a c a g a t t t c t c a t t a t g a t c t c t a t t t g t a t t

5940 2μ ori a a a a t g t a g a g g t c g a g t t t a g a t g c a a g t t c a a g g a g c g a a a g g t g g a t g g g t a g g t t a t t t t a c a t c t c c a g c t c a a a t c t a c g t t c a a g t t c c t c g c t t t c c a c c t a c c c a t c c a a t

6000 2μ ori t a t a g g g a t a t a g c a c a g a g a t a t a t a g c a a a g a g a t a c t t t t g a g c a a t g t t t g t g g a a a t a t c c c t a t a t c g t g t c t c t a t a t a t c g t t t c t c t a t g a a a a c t c g t t a c a a a c a c c t t

6060 2μ ori g c g g t a t t c g c a a t a t t t t a g t a g c t c g t t a c a g t c c g g t g c g t t t t t g g t t t t t t g a a a c g c c a t a a g c g t t a t a a a a t c a t c g a g c a a t g t c a g g c c a c g c a a a a a c c a a a a a a c t t t

6120 2μ ori FRT g t g c g t c t t c a g a g c g c t t t t g g t t t t c a a a a g c g c t c t g a a g t t c c t a t a c t t t c t a g a c a c g c a g a a g t c t c g c g a a a a c c a a a a g t t t t c g c g a g a c t t c a a g g a t a t g a a a g a t c t

6180 2μ ori FRT g a a t a g g a a c t t c g g a a t a g g a a c t t c a a a g c g t t t c c g a a a a c g a g c g c t t c c g a a a a t c t t a t c c t t g a a g c c t t a t c c t t g a a g t t t c g c a a a g g c t t t t g c t c g c g a a g g c t t t t a

BmgBI 6240 2μ ori g c a a c g c g a g c t g c g c a c a t a c a g c t c a c t g t t c a c g t c g c a c c t a t a t c t g c g t g t t g c c g t t g c g c t c g a c g c g t g t a t g t c g a g t g a c a a g t g c a g c g t g g a t a t a g a c g c a c a a c g

6300 2μ ori c t g t a t a t a t a t a t a c a t g a g a a g a a c g g c a t a g t g c g t g t t t a t g c t t a a a t g c g t a c t g a c a t a t a t a t a t a t g t a c t c t t c t t g c c g t a t c a c g c a c a a a t a c g a a t t t a c g c a t g a

6360 2μ ori t a t a t g c g t c t a t t t a t g t a g g a t g a a a g g t a g t c t a g t a c c t c c t g t g a t a t t a t c c c a a t a t a c g c a g a t a a a t a c a t c c t a c t t t c c a t c a g a t c a t g g a g g a c a c t a t a a t a g g g t

6420 2μ ori t t c c a t g c g g g g t a t c g t a t g c t t c c t t c a g c a c t a c c c t t t a g c t g t t c t a t a t g c t g c a a g g t a c g c c c c a t a g c a t a c g a a g g a a g t c g t g a t g g g a a a t c g a c a a g a t a t a c g a c g

6480 2μ ori c a c t c c t c a a t t g g a t t a g t c t c a t c c t t c a a t g c t a t c a t t t c c t t t g a t a t t g g a t c a g t g a g g a g t t a a c c t a a t c a g a g t a g g a a g t t a c g a t a g t a a a g g a a a c t a t a a c c t a g t

6540 A A T A G G C G T A T C A C G A G G C pBRforEco t a c t a a g a a a c c a t t a t t a t c a t g a c a t t a a c c t a t a a a a a t a g g c g t a t c a c g a g g c c c a t g a t t c t t t g g t a a t a a t a g t a c t g t a a t t g g a t a t t t t t a t c c g c a t a g t g c t c c g g g

PfoI 6600 G G A G A C T G T G T A C G T C G A G G G C C pGEX 3' t t t c g t c t c g c g c g t t t c g g t g a t g a c g g t g a a a a c c t c t g a c a c a t g c a g c t c c c g g a g a a a g c a g a g c g c g c a a a g c c a c t a c t g c c a c t t t t g g a g a c t g t g t a c g t c g a g g g c c t c

6660 a c g g t c a c a g c t t g t c t g t a a g c g g a t g c c g g g a g c a g a c a a g c c c g t c a g g g c g c g t c a t g c c a g t g t c g a a c a g a c a t t c g c c t a c g g c c c t c g t c t g t t c g g g c a g t c c c g c g c a g t

6720 C G G C A T C A G A G C A G A T T G T A pRS-marker g c g g g t g t t g g c g g g t g t c g g g g c t g g c t t a a c t a t g c g g c a t c a g a g c a g a t t g t a c t g c g c c c a c a a c c g c c c a c a g c c c c g a c c g a a t t g a t a c g c c g t a g t c t c g t c t a a c a t g a c

6780 a g a g t g c a c c a t a t c g a c t a c g t c g t t a a g g c c g t t t c t g a c a g a g t a a a a t t c t t g a g g t c t c a c g t g g t a t a g c t g a t g c a g c a a t t c c g g c a a a g a c t g t c t c a t t t t a a g a a c t c c

6840 g a a c t t t c a c c a t t a t g g g a a a t g g t t c a a g a a g g t a t t g a c t t a a a c t c c a t c a a a t g g c t t g a a a g t g g t a a t a c c c t t t a c c a a g t t c t t c c a t a a c t g a a t t t g a g g t a g t t t a c c

6900 t c a g g t c a t t g a g t g t t t t t t a t t t g t t g t a t t t t t t t t t t t t t a g a g a a a a t c c t c c a a a g t c c a g t a a c t c a c a a a a a a t a a a c a a c a t a a a a a a a a a a a a a t c t c t t t t a g g a g g t t

6960 t a t a t a a a t t a g g a a t c a t a g t t t c a t g a t t t t c t g t t a c a c c t a a c t t t t t g t g t g g t g a t a t a t t t a a t c c t t a g t a t c a a a g t a c t a a a a g a c a a t g t g g a t t g a a a a a c a c a c c a c

7020 c c c t c c t c c t t g t c a a t a t t a a t g t t a a a g t g c a a t t c t t t t t c c t t a t c a c g t t g a g c c g g g a g g a g g a a c a g t t a t a a t t a c a a t t t c a c g t t a a g a a a a a g g a a t a g t g c a a c t c g g

7080 a t t a g t a t c a a t t t g c t t a c c t g t a t t c c t t t a c a t c c t c c t t t t t c t c c t t c t t g a t a a t a a t c a t a g t t a a a c g a a t g g a c a t a a g g a a a t g t a g g a g g a a a a a g a g g a a g a a c t a t t

7140 a t g t a t g t a g a t t g c g t a t a t a g t t t c g t c t a c c c t a t g a a c a t a t t c c a t t t t g t a a t t t a c a t a c a t c t a a c g c a t a t a t c a a a g c a g a t g g g a t a c t t g t a t a a g g t a a a a c a t t a a

7200 t c g t g t c g t t t c t a t t a t g a a t t t c a t t t a t a a a g t t t a t g t a c a a a t a t c a t a a a a a a a a g c a c a g c a a a g a t a a t a c t t a a a g t a a a t a t t t c a a a t a c a t g t t t a t a g t a t t t t t t t

7260 365 360 355 350 *ALIKKVEEAVADGVET LEU2 *ALIKKVEEAVADGVET g a g a a t c t t t t t a a g c a a g g a t t t t c t t a a c t t c t t c g g c g a c a g c a t c a c c g a c t t c g g c t c t t a g a a a a a t t c g t t c c t a a a a g a a t t g a a g a a g c c g c t g t c g t a g t g g c t g a a g c c

7320 345 340 335 330 TSNSGGLDGTRIGADLVKKV LEU2 TSNSGGLDGTRIGADLVKKV t g g t a c t g t t g g a a c c a c c t a a a t c a c c a g t t c t g a t a c c t g c a t c c a a a a c c t t t t t a a a c c a t g a c a a c c t t g g t g g a t t t a g t g g t c a a g a c t a t g g a c g t a g g t t t t g g a a a a a t t

7380 325 320 315 310 ADEIAKGEEPLNLSLKLMMA LEU2 ADEIAKGEEPLNLSLKLMMA c t g c a t c t t c a a t g g c c t t a c c t t c t t c a g g c a a g t t c a a t g a c a a t t t c a a c a t c a t t g g a c g t a g a a g t t a c c g g a a t g g a a g a a g t c c g t t c a a g t t a c t g t t a a a g t t g t a g t a a c

7440 305 300 295 290 ASLITAIPNVKNKPLDPASG LEU2 ASLITAIPNVKNKPLDPASG c a g c a g a c a a g a t a g t g g c g a t a g g g t t g a c c t t a t t c t t t g g c a a a t c t g g a g c a g a a c g t c g t c t g t t c t a t c a c c g c t a t c c c a a c t g g a a t a a g a a a c c g t t t a g a c c t c g t c t t g

7500 285 280 275 270 HCPEYLGFATNKDPLSALSA LEU2 HCPEYLGFATNKDPLSALSA c g t g g c a t g g t t c g t a c a a a c c a a a t g c g g t g t t c t t g t c t g g c a a a g a g g c c a a g g a c g g c a c c g t a c c a a g c a t g t t t g g t t t a c g c c a c a a g a a c a g a c c g t t t c t c c g g t t c c t g c

7560 265 260 255 250 SPLLGLSGPIVSAEDSIIDG LEU2 SPLLGLSGPIVSAEDSIIDG c a g a t g g c a a c a a a c c c a a g g a a c c t g g g a t a a c g g a g g c t t c a t c g g a g a t g a t a t c a c g t c t a c c g t t g t t t g g g t t c c t t g g a c c c t a t t g c c t c c g a a g t a g c c t c t a c t a t a g t g

7620 245 240 235 230 FMNSTIIIGNLHTPNKVLIM LEU2 FMNSTIIIGNLHTPNKVLIM c a a a c a t g t t g c t g g t g a t t a t a a t a c c a t t t a g g t g g g t t g g g t t c t t a a c t a g g a t c a g t t t g t a c a a c g a c c a c t a a t a t t a t g g t a a a t c c a c c c a a c c c a a g a a t t g a t c c t a g t

EcoRI 7680 225 220 215 210 AASDILQHQVKLTPFENKIT LEU2 AASDILQHQVKLTPFENKIT t g g c g g c a g a a t c a a t c a a t t g a t g t t g a a c c t t c a a t g t a g g g a a t t c g t t c t t g a t g g a c c g c c g t c t t a g t t a g t t a a c t a c a a c t t g g a a g t t a c a t c c c t t a a g c a a g a a c t a c c

7740 205 200 195 190 EEVTKRWLRSSALVNAKDLS LEU2 EEVTKRWLRSSALVNAKDLS t t t c c t c c a c a g t t t t t c t c c a t a a t c t t g a a g a g g c c a a a a c a t t a g c t t t a t c c a a g g a a a g g a g g t g t c a a a a a g a g g t a t t a g a a c t t c t c c g g t t t t g t a a t c g a a a t a g g t t c c

7800 185 180 175 170 WIPLPPEHQLAMFAAMRTIR LEU2 WIPLPPEHQLAMFAAMRTIR a c c a a a t a g g c a a t g g t g g c t c a t g t t g t a g g g c c a t g a a a g c g g c c a t t c t t g t g a t t c t g g t t t a t c c g t t a c c a c c g a g t a c a a c a t c c c g g t a c t t t c g c c g g t a a g a a c a c t a a g

7860 165 160 155 150 QVEPVTYQESDWAVGDGDDE LEU2 QVEPVTYQESDWAVGDGDDE t t t g c a c t t c t g g a a c g g t g t a t t g t t c a c t a t c c c a a g c g a c a c c a t c a c c a t c g t c t t a a a c g t g a a g a c c t t g c c a c a t a a c a a g t g a t a g g g t t c g c t g t g g t a g t g g t a g c a g a a

7920 145 140 135 130 KRKGFYIGGVLERVVVFDTG LEU2 KRKGFYIGGVLERVVVFDTG c c t t t c t c t t a c c a a a g t a a a t a c c t c c c a c t a a t t c t c t g a c a a c a a c g a a g t c a g t a c g g a a a g a g a a t g g t t t c a t t t a t g g a g g g t g a t t a a g a g a c t g t t g t t g c t t c a g t c a t g

7980 125 120 115 110 KAFQPKIPSLDLLSDSAFNC LEU2 KAFQPKIPSLDLLSDSAFNC c t t t a g c a a a t t g t g g c t t g a t t g g a g a t a a g t c t a a a a g a g a g t c g g a t g c a a a g t t a c g a a a t c g t t t a a c a c c g a a c t a a c c t c t a t t c a g a t t t t c t c t c a g c c t a c g t t t c a a t g

8040 105 100 95 90 PRLNAYLQLEKRIKLLGQEP LEU2 PRLNAYLQLEKRIKLLGQEP a t g g t c t t a a g t t g g c g t a c a a t t g a a g t t c t t t a c g g a t t t t t a g t a a a c c t t g t t c a g t a c c a g a a t t c a a c c g c a t g t t a a c t t c a a g a a a t g c c t a a a a a t c a t t t g g a a c a a g t c

8100 85 80 75 70 RVSGTGWKPGGVAGLLVADA LEU2 RVSGTGWKPGGVAGLLVADA g t c t a a c a c t a c c g g t a c c c c a t t t a g g a c c a c c c a c a g c a c c t a a c a a a a c g g c a t c a g c a g a t t g t g a t g g c c a t g g g g t a a a t c c t g g t g g g t g t c g t g g a t t g t t t t g c c g t a g t c

8160 65 60 55 50 KKSAELAEDPLPVGTADIAA LEU2 KKSAELAEDPLPVGTADIAA c c t t c t t g g a g g c t t c c a g c g c c t c a t c t g g a a g t g g a a c a c c t g t a g c a t c g a t a g c a g g g a a g a a c c t c c g a a g g t c g c g g a g t a g a c c t t c a c c t t g t g g a c a t c g t a g c t a t c g t c

8220 45 40 35 30 GGILHNEFDFKVNSRVDSIA LEU2 GGILHNEFDFKVNSRVDSIA c a c c a c c a a t t a a a t g a t t t t c g a a a t c g a a c t t g a c a t t g g a a c g a a c a t c a g a a a t a g g t g g t g g t t a a t t t a c t a a a a g c t t t a g c t t g a a c t g t a a c c t t g c t t g t a g t c t t t a t c

8280 25 20 15 10 KLVKIAEATIEQGVHDGPLV LEU2 KLVKIAEATIEQGVHDGPLV c t t t a a g a a c c t t a a t g g c t t c g g c t g t g a t t t c t t g a c c a a c g t g g t c a c c t g g c a a a a g a a a t t c t t g g a a t t a c c g a a g c c g a c a c t a a a g a a c t g g t t g c a c c a g t g g a c c g t t t t

8340 5 1 VIKKPASM LEU2 LEU2 promoter VIKKPASM c g a c g a t c t t c t t a g g g g c a g a c a t t a g a a t g g t a t a t c c t t g a a a t a t a t a t a t a t a t a g c t g c t a g a a g a a t c c c c g t c t g t a a t c t t a c c a t a t a g g a a c t t t a t a t a t a t a t a t a t

8400 LEU2 promoter t t g c t g a a a t g t a a a a g g t a a g a a a a g t t a g a a a g t a a g a c g a t t g c t a a c c a c c t a t t g a a c g a c t t t a c a t t t t c c a t t c t t t t c a a t c t t t c a t t c t g c t a a c g a t t g g t g g a t a a c

8460 LEU2 promoter g a a a a a a c a a t a g g t c c t t a a a t a a t a t t g t c a a c t t c a a g t a t t g t g a t g c a a g c a t t t c t t t t t t g t t a t c c a g g a a t t t a t t a t a a c a g t t g a a g t t c a t a a c a c t a c g t t c g t a a a

8520 LEU2 promoter a g t c a t g a a c g c t t c t c t a t t c t a t a t g a a a a g c c g g t t c c g g c g c t c t c a c c t t t c c t t t c a g t a c t t g c g a a g a g a t a a g a t a t a c t t t t c g g c c a a g g c c g c g a g a g t g g a a a g g a a

8580 LEU2 promoter t t t c t c c c a a t t t t t c a g t t g a a a a a g g t a t a t g c g t c a g g c g a c c t c t g a a a t t a a c a a a a a g a g g g t t a a a a a g t c a a c t t t t t c c a t a t a c g c a g t c c g c t g g a g a c t t t a a t t g t t

8640 LEU2 promoter a a a a t t t c c a g t c a t c g a a t t t g a t t c t g t g c g a t a g c g c c c c t g t g t g t t c t c g t t a t g t t t t a a a g g t c a g t a g c t t a a a c t a a g a c a c g c t a t c g c g g g g a c a c a c a a g a g c a a t a c

8700 LEU2 promoter t t g a g g a a a a a a a t a a t g g t t g c t a a g a g a t t c g a a c t c t t g c a t c t t a c g a t a c c t g a g a a c t c c t t t t t t t a t t a c c a a c g a t t c t c t a a g c t t g a g a a c g t a g a a t g c t a t g g a c t c

KasINarISfoIPluTIHpaI 8760 LEU2 promoter t a t t c c c a c a g t t a a c t g c g g t c a a g a t a t t t c t t g a a t c a g g c g c c t t a g a c c g c t c g g a t a a g g g t g t c a a t t g a c g c c a g t t c t a t a a a g a a c t t a g t c c g c g g a a t c t g g c g a g c c

8820 c c a a a c a a c c a a t t a c t t g t t g a g a a a t a g a g t a t a a t t a t c c t a t a a a t a t a a c g t t t t g g t t t g t t g g t t a a t g a a c a a c t c t t t a t c t c a t a t t a a t a g g a t a t t t a t a t t g c a a a a

8880 t g a a c a c a c a t g a a c a a g g a a g t a c a g g a c a a t t g a t t t t g a a g a g a a t g t g g a t t t t g a a c t t g t g t g t a c t t g t t c c t t c a t g t c c t g t t a a c t a a a a c t t c t c t t a c a c c t a a a a c t

8940 t g t a a t t g t t g g g a t t c c a t t t t t a a t a a g g c a a t a a t a t t a g g t a t g t a g a t a t a c t a g a c a t t a a c a a c c c t a a g g t a a a a a t t a t t c c g t t a t t a t a a t c c a t a c a t c t a t a t g a t c

9000 a a g t t c t c c t c g a c c g g t c g a t a t g c g g t g t g a a a t a c c g c a c a g a t g c g t a a g g a g a a a t t c a a g a g g a g c t g g c c a g c t a t a c g c c a c a c t t t a t g g c g t g t c t a c g c a t t c c t c t t t

9060 f1 ori a t a c c g c a t c a g g a a a t t g t a a g c g t t a a t a t t t t g t t a a a a t t c g c g t t a a a t t t t t g t t a t g g c g t a g t c c t t t a a c a t t c g c a a t t a t a a a a c a a t t t t a a g c g c a a t t t a a a a a c a

9120 f1 ori t a a a t c a g c t c a t t t t t t a a c c a a t a g g c c g a a a t c g g c a a a a t c c c t t a t a a a t c a a a a a t t t a g t c g a g t a a a a a a t t g g t t a t c c g g c t t t a g c c g t t t t a g g g a a t a t t t a g t t t t

9180 f1 ori G G T C A A A C C T T G T T C T C A G G T G F1ori-F g a a t a g a c c g a g a t a g g g t t g a g t g t t g t t c c a g t t t g g a a c a a g a g t c c a c t a t t a a a g c t t a t c t g g c t c t a t c c c a a c t c a c a a c a a g g t c a a a c c t t g t t c t c a g g t g a t a a t t t c

DraIII 9240 f1 ori a a c g t g g a c t c c a a c g t c a a a g g g c g a a a a a c c g t c t a t c a g g g c g a t g g c c c a c t a c g t t t g c a c c t g a g g t t g c a g t t t c c c g c t t t t t g g c a g a t a g t c c c g c t a c c g g g t g a t g c a

9300 f1 ori g a a c c a t c a c c c t a a t c a a g t t t t t t g g g g t c g a g g t g c c g t a a a g c a c t a a a t c g g a a c c t t g g t a g t g g g a t t a g t t c a a a a a a c c c c a g c t c c a c g g c a t t t c g t g a t t t a g c c t t g

NgoMIVNaeI 9360 f1 ori c c t a a a g g g a g c c c c c g a t t t a g a g c t t g a c g g g g a a a g c c g g c g a a c g t g g c g a g a a a g g g a t t t c c c t c g g g g g c t a a a t c t c g a a c t g c c c c t t t c g g c c g c t t g c a c c g c t c t t t c

9420 f1 ori A G G G A A G A A A G C G A A A G G A G F1ori-R g a a g g g a a g a a a g c g a a a g g a g c g g g c g c t a g g g c g c t g g c a a g t g t a g c g g t c a c g c t g c t t c c c t t c t t t c g c t t t c c t c g c c c g c g a t c c c g c g a c c g t t c a c a t c g c c a g t g c g a c

9480 f1 ori c g c g t a a c c a c c a c a c c c g c c g c g c t t a a t g c g c c g c t a c a g g g c g c g t c c a t t c g c c a t g c g c a t t g g t g g t g t g g g c g g c g c g a a t t a c g c g g c g a t g t c c c g c g c a g g t a a g c g g t a

9540 t c a g g c t g c g c a a c t g t t g g g a a g g g c g a t c g g t g c g g g c c t c t t c g c t a t t a c g c c a g c a g t c c g a c g c g t t g a c a a c c c t t c c c g c t a g c c a c g c c c g g a g a a g c g a t a a t g c g g t c g

9600 C C C A G T M13/pUC Forward t g g c g a a a g g g g g a t g t g c t g c a a g g c g a t t a a g t t g g g t a a c g c c a g g g t t t t c c c a g t a c c g c t t t c c c c c t a c a c g a c g t t c c g c t a a t t c a a c c c a t t g c g g t c c c a a a a g g g t c a

9660 M13 fwd T7 promoter C A C G A C G T T G T A A A A C G M13/pUC Forward T G T A A A A C G A C G G C C A G T M13 Forward T A A T A C G A C T C A C T A T A G G G T7 c a c g a c g t t g t a a a a c g a c g g c c a g t g a g c g c g c g t a a t a c g a c t c a c t a t a g g g c g a a t g t g c t g c a a c a t t t t g c t g c c g g t c a c t c g c g c g c a t t a t g c t g a g t g a t a t c c c g c t t a

PspOMIApaI 9720 t g g g t a c c g g g c c c a t t g t a g c a a a g a t t g t a a g g a a a t a g c a a a c c a g a g g t c c a a g t c a c c c a t g g c c c g g g t a a c a t c g t t t c t a a c a t t c c t t t a t c g t t t g g t c t c c a g g t t c a g

9780 a a a a c g g c a a a a a a g a a g a a a g t a a g c a a t a t a a t g g a c g g g t t t a c a t g t t t a a a t a t a t t t t g c c g t t t t t t c t t c t t t c a t t c g t t a t a t t a c c t g c c c a a a t g t a c a a a t t t a t a t

9840 t g t a c a t g t a t g t a g t c t t a t a c t t t c a a a a c a t a a t c g c a t g c g g g t c a t c a a a a t a a a a c a t g t a c a t a c a t c a g a a t a t g a a a g t t t t g t a t t a g c g t a c g c c c a g t a g t t t t a t t t

9900 c c t c c a t t t t g g a c t t g g g a a a a a g a a a a t t t g a t t t a a a c t g t t g t a t g t a g c t a a c a a g g a g g t a a a a c c t g a a c c c t t t t t c t t t t a a a c t a a a t t t g a c a a c a t a c a t c g a t t g t t

SwaI 9960 t a c a t a a a a c t a t t t a a a t a a a a t a g g a a t a a g a a t g c t c a a t t a a a a a a t g a t t t a t t t a t g t a t t t t g a t a a a t t t a t t t t a t c c t t a t t c t t a c g a g t t a a t t t t t t a c t a a a t a a a

SbfIPstI 10,020 *KSRKYVKNLF t c t a a c a a a a c t t c c t g c a g g t c a c t a t t a t t t g c t t c t c t t g t a a a c t t t g t t c a a a a a a g a t t g t t t t g a a g g a c g t c c a g t g a t a a t a a a c g a a g a g a a c a t t t g a a a c a a g t t t t t

10,080 ATLVDAKFGRSEDVQSIKAK c g c a g t t a a g a c g t c g g c t t t g a a g c c a c g a g a c t c g t c g a c t t g g g a g a t c t t g g c c t t g c g t c a a t t c t g c a g c c g a a a c t t c g g t g c t c t g a g c a g c t g a a c c c t c t a g a a c c g g a a

10,140 LDKAVSEEYEHYLQDIKLDN c a a a t c c t t g g c a a c a g a c t c t t c a t a t t c g t g g t a t a a c t g g t c g a t t t t c a a g t c a t t g t t t a g g a a c c g t t g t c t g a g a a g t a t a a g c a c c a t a t t g a c c a g c t a a a a g t t c a g t a a

10,200 FIKKCKAEAVSDKKGYNEDL g a a a a t c t t t t t g c a t t t g g c t t c t g c g a c t g a g t c c t t c t t a c c g t a a t t t t c g t c t a a c t t t t a g a a a a a c g t a a a c c g a a g a c g c t g a c t c a g g a a g a a t g g c a t t a a a a g c a g a t t

10,260 TKRQEASALELAKNIVWSCK a g t c t t t c t t t g t t c t g c g g a a g c a a g t t c t a a t g c c t t g t t g a t t a c c c a a g a a c a t t t t c a g a a a g a a a c a a g a c g c c t t c g t t c a a g a t t a c g g a a c a a c t a a t g g g t t c t t g t a a a

10,320 NDQIDTGIKGIQEPTGFCDL g t t a t c t t g g a t a t c t g t a c c g a t c t t a c c g a t c t g t t c t g g g g t a c c g a a g c a g t c t a a c a a t a g a a c c t a t a g a c a t g g c t a g a a t g g c t a g a c a a g a c c c c a t g g c t t c g t c a g a t t

10,380 YDDQIQFYEGLPILVDRAQK g t a g t c a t c t t g a a t t t g g a a a t a t t c a c c c a a t g g a a t c a a g a c a t c t c t g g c t t g t t t c a t c a g t a g a a c t t a a a c c t t t a t a a g t g g g t t a c c t t a g t t c t g t a g a g a c c g a a c a a a

10,440 LDKEDTIGAVYMALAVPLYF c a a a t c c t t t t c a t c t g t g a t a c c g g c a a c g t a c a t a g c c a a t g c g a c a g g c a a g t a g a a g t t t a g g a a a a g t a g a c a c t a t g g c c g t t g c a t g t a t c g g t t a c g c t g t c c g t t c a t c t t

10,500 SYYATKFTVIFSHKKLSFKS a g a a t a g t a a g c a g t c t t g a a a g t a a c t a t g a a g g a g t g c t t c t t t a g g g a g a a c t t a c t t c t t a t c a t t c g t c a g a a c t t t c a t t g a t a c t t c c t c a c g a a g a a a t c c c t c t t g a a t g a

BsgI 10,560 LDVKDEPATILDMLQGLETQ c a a g t c g a c t t t g t c t t c a g g t g c a g t g a t t a a g t c c a t c a a t t g g c c c a a t t c g g t t t g g t t c a g c t g a a a c a g a a g t c c a c g t c a c t a a t t c a g g t a g t t a a c c g g g t t a a g c c a a a c

10,620 FTVEHFLETIDIYYKENRFH g a a g g t g a c t t c a t g g a a c a a t t c g g t g a t a t c t a t g t a g t a t t t t t c g t t t c t g a a g t g c t t c c a c t g a a g t a c c t t g t t a a g c c a c t a t a g a t a c a t c a t a a a a a g c a a a g a c t t c a c

10,680 SKLLKYIAAELMFADNIAIE a g a t t t c a a a a g c t t g t a g a t a g c a g c c t c t a a c a t g a a t g c g t c a t t g a t g g c a a t t t c t c t a a a g t t t t c g a a c a t c t a t c g t c g g a g a t t g t a c t t a c g c a g t a a c t a c c g t t a a a g

10,740 GVEPVKYWCPQGRRTISKDM c c c a a c t t c a g g a a c c t t g t a c c a a c a t g g t t g g c c t c t t c t g g t a a t g g a c t t g t c c a t g g g t t g a a g t c c t t g g a a c a t g g t t g t a c c a a c c g g a g a a g a c c a t t a c c t g a a c a g g t a

10,800 MDDAVLFYAQLLEICWGLIA c a t a t c a t c g g c g a c c a a g a a g t a a g c c t g c a a c a a c t c a a t g c a c c a a c c t a g a a t a g c g t a t a g t a g c c g c t g g t t c t t c a t t c g g a c g t t g t t g a g t t a c g t g g t t g g a t c t t a t c g

10,860 VKEYEEQGLQEVTKNSLIAY a a c c t t t t c g t a t t c t t c t t g c c c c a a t t g t t c a a c g g t c t t g t t g g a g a g a a t a g c a t a t t g g a a a a g c a t a a g a a g a a c g g g g t t a a c a a g t t g c c a g a a c a a c c t c t c t t a t c g t a t

10,920 TDVVSLGRNLKGGPTNYNLS c g t g t c c a c a a c g g a c a a a c c t c t a t t t a a c t t a c c g c c t g g a g t g t t g t a g t t c a a t g a g c a c a g g t g t t g c c t g t t t g g a g a t a a a t t g a a t g g c g g a c c t c a c a a c a t c a a g t t a c t

Bsu36I 10,980 HAYWDCAEKPMGYALLSANL g t g g g c a t a c c a g t c a c a t g c t t c c t t a g g c a t a c c g t a a g c c a a a a g c g a t g c g t t c a a c a c c c g t a t g g t c a g t g t a c g a a g g a a t c c g t a t g g c a t t c g g t t t t c g c t a c g c a a g t t

11,040 EEVLKPFVNLFRERRIEKES t t c c t c t a c t a a t t t a g g g a a a a c g t t c a a g a a t c t c t c t c t c c t a a t t t c t t t t t c t g a a a g g a g a t g a t t a a a t c c c t t t t g c a a g t t c t t a g a g a g a g a g g a t t a a a g a a a a a g a c t

BmtINheI 11,100 AM a g c c a t t t t g c t a g c t t t a t g t g a t g a t t g a t t g a t t g a t t g t a c a g t t t g t t t t t c t t a t c g g t a a a a c g a t c g a a a t a c a c t a c t a a c t a a c t a a c t a a c a t g t c a a a c a a a a a g a a t

11,160 CUP1 promoter a t a t c t a t t t c g a t g a c t t c t a t a t g a t a t t g c a c t a a c a a g a a g a t a t t a t a a t g c a a t t a t a g a t a a a g c t a c t g a a g a t a t a c t a t a a c g t g a t t g t t c t t c t a t a a t a t t a c g t t a

3ʹ 5ʹ 11,185 CUP1 promoter t g a t a c a a g a c a a g g a g t t a t t t g c a c t a t g t t c t g t t c c t c a a t a a a c g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.