Addgene pCMV6M-Pak1 H83L H86L Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pCMV6M-Pak1 H83L H86L Sequencing Result

Map View

600 400 200 End (796) BmgBI (765) AvaI - BsoBI (758) TsoI (757) BstZ17I (717) PstI (624) SfcI (620) BbvCI - Bpu10I (566) PvuII - MspA1I (565) BbsI (526) ApaI BaeGI Bme1580I (429) PspOMI (425) BstXI (422) BsmI (416) SacI (365) Eco53kI (363) EaeI (331) BsrBI (330) BpmI (317) TaqII (287) BseRI (268) BtgI StyI NcoI (222) NaeI (195) BsrFI NgoMIV (193) PspFI (157) BseYI (153) BamHI (112) XbaI (106) EarI (89) HincII (69) SalI (67) PvuI - BsiEI (65) HindIII (56) ClaI - BspDI (51) MluI - AflIII (44) KpnI (42) BanI - Acc65I (38) BglII (32) AseI (25) Start (0) Myc 6xHis pCMV6M-Pak1 H83L H86L 796 bp

Sequence View

HindIIIBspDIClaIBglIIAcc65IBanIKpnIAflIIIMluIAseIStart (0) 5ʹ 3ʹ 60 a g c t c g t t t a g t g a a c c g t c a g a a t t a a t t c a g a t c t g g t a c c a c g c g t a t c g a t a a g c t t c g a g c a a a t c a c t t g g c a g t c t t a a t t a a g t c t a g a c c a t g g t g c g c a t a g c t a t t c g a

BamHIXbaIEarIBsiEIPvuISalIHincII 120 1 5 10 EQKLISEEDL (in frame with Myc) SRGSM Myc MEQKLISEEDLSRGSM t c g a t c g t c g a c a t g g a a c a g a a a c t c a t c t c t g a a g a g g a t c t g t c t a g a g g a t c c a t g a g c t a g c a g c t g t a c c t t g t c t t t g a g t a g a g a c t t c t c c t a g a c a g a t c t c c t a g g t a c

BseYIPspFI 180 (in frame with Myc) SNNGLDIQDKPPAPPMRNTS SNNGLDIQDKPPAPPMRNTS t c a a a t a a c g g c c t a g a c a t t c a a g a c a a a c c c c c a g c c c c t c c g a t g a g a a a t a c c a g c a g t t t a t t g c c g g a t c t g t a a g t t c t g t t t g g g g g t c g g g g a g g c t a c t c t t t a t g g t c g

NcoIStyIBtgINgoMIVBsrFINaeI 240 (in frame with Myc) TMIGAGSKDAGTLNHGSKPL TMIGAGSKDAGTLNHGSKPL a c t a t g a t t g g a g c c g g c a g c a a a g a t g c t g g a a c c c t a a a c c a t g g t t c t a a a c c t c t g t g a t a c t a a c c t c g g c c g t c g t t t c t a c g a c c t t g g g a t t t g g t a c c a a g a t t t g g a g a c

TaqIIBseRI 300 (in frame with Myc) PPNPEEKKKKDRFYRSILPG PPNPEEKKKKDRFYRSILPG c c t c c a a a c c c a g a g g a g a a g a a a a a g a a g g a c c g a t t t t a c c g a t c c a t t t t a c c t g g a g g a g g t t t g g g t c t c c t c t t c t t t t t c t t c c t g g c t a a a a t g g c t a g g t a a a a t g g a c c t

BsrBIEaeIBpmI 360 (in frame with Myc) DKTNKKKEKERPEISLPSDF DKTNKKKEKERPEISLPSDF g a t a a a a c a a a t a a a a a g a a a g a g a a a g a g c g g c c a g a g a t t t c t c t c c c t t c a g a t t t t c t a t t t t g t t t a t t t t t c t t t c t c t t t c t c g c c g g t c t c t a a a g a g a g g g a a g t c t a a a a

BsmIEco53kISacI 420 (in frame with Myc) ELTILVGFDAVTGEFTGMPE ELTILVGFDAVTGEFTGMPE g a g c t c a c a a t t c t t g t c g g t t t t g a t g c t g t c a c a g g g g a g t t t a c g g g a a t g c c a g a g c t c g a g t g t t a a g a a c a g c c a a a a c t a c g a c a g t g t c c c c t c a a a t g c c c t t a c g g t c t c

BstXIPspOMIBme1580IBaeGIApaI 480 (in frame with Myc) QWARLLQTSNITKSEQKKNP QWARLLQTSNITKSEQKKNP c a g t g g g c c c g c t t g c t t c a g a c a t c a a a t a t c a c t a a g t c g g a g c a g a a g a a a a a c c c g g t c a c c c g g g c g a a c g a a g t c t g t a g t t t a t a g t g a t t c a g c c t c g t c t t c t t t t t g g g c

BbsI 540 (in frame with Myc) QAVLDVLEFYNSKKTSNSQK QAVLDVLEFYNSKKTSNSQK c a g g c t g t t c t g g a t g t g t t g g a g t t t t a c a a c t c g a a g a a g a c a t c c a a c a g c c a g a a a g t c c g a c a a g a c c t a c a c a a c c t c a a a a t g t t g a g c t t c t t c t g t a g g t t g t c g g t c t t t

MspA1IPvuIIBpu10IBbvCI 600 (in frame with Myc) YMSFTDKSAEDYNSSNALNV (in frame with 6xHis) *HRSHS YMSFTDKSAEDYNSSNALNV t a c a t g a g c t t t a c a g a t a a g t c a g c t g a g g a t t a c a a t t c t t c t a a t g c c t t g a a t g t g a t g t a c t c g a a a t g t c t a t t c a g t c g a c t c c t a a t g t t a a g a a g a t t a c g g a a c t t a c a c

PstISfcI 660 (in frame with Myc) KAVSETPAVPPVSEDEDDDD (in frame with 6xHis) PQTQSEQLAVLKLLHP 5 HHHH 6xHis KAVSETPAVPPVSEDEDDDD a a g g c t g t g t c t g a g a c t c c t g c a g t g c c a c c a g t t t c a g a a g a t g a g g a t g a t g a t g a t t t c c g a c a c a g a c t c t g a g g a c g t c a c g g t g g t c a a a g t c t t c t a c t c c t a c t a c t a c t a

BstZ17I 720 (in frame with Myc) DDATPPPVIAPRPEHTKSVY 1 HH 6xHis DDATPPPVIAPRPEHTKSVY g a t g a t g c t a c c c c a c c a c c a g t g a t t g c t c c a c g c c c a g a g c a c a c a a a a t c t g t a t a c c t a c t a c g a t g g g g t g g t g g t c a c t a a c g a g g t g c g g g t c t c g t g t g t t t t a g a c a t a t g

BmgBITsoIBsoBIAvaI 780 (in frame with Myc) TRSVIEPLPVTPTRDVATSP TRSVIEPLPVTPTRDVATSP a c a c g g t c t g t g a t t g a a c c a c t t c c t g t c a c t c c a a c t c g g g a c g t g g c t a c a t c t c c c t g t g c c a g a c a c t a a c t t g g t g a a g g a c a g t g a g g t t g a g c c c t g c a c c g a t g t a g a g g g

End (796) 3ʹ 5ʹ 796 (in frame with Myc) ISPTE ISPTE a t t t c a c c t a c t g a a a t a a a g t g g a t g a c t t t

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.