Addgene RPS6KA5 gRNA (BRDN0001145521) Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: RPS6KA5 gRNA (BRDN0001145521) Sequencing Result


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PspXI (8314) NdeI (7931) LKO.1 5' (7924 .. 7943) hU6-F (7753 .. 7773) KflI - PpuMI (7744) gp41 peptide AleI (7387) NotI (6958) NruI * (6647) SphI (6131) T3 promoter T3 (5993 .. 6013) M13 rev M13 Reverse (5958 .. 5974) lac operator M13/pUC Reverse (5939 .. 5961) lac promoter CAP binding site L4440 (5726 .. 5743) pBR322ori-F (5473 .. 5492) AhdI (4740) ScaI (4259) TspMI - XmaI (1) SmaI (3) BfuAI - BspMI (611) FseI (791) EF1a-F (1213 .. 1233) Puro-R (1278 .. 1297) PflFI - Tth111I (1320) BspEI * (1391) RsrII (1394) BstEII (1412) MscI * (1653) Puro-F (1774 .. 1794) CsiI - SexAI * (1845) MluI (1878) WPRE-R (1946 .. 1966) PflMI (2134) SnaBI (2790) SV40pA-R (2901 .. 2920) EBV-rev (2955 .. 2974) NcoI (3066) SfiI (3112) AvrII (3159) T7 promoter T7 (3180 .. 3199) M13 fwd M13 Forward (3209 .. 3226) M13/pUC Forward (3218 .. 3240) F1ori-R (3454 .. 3473) F1ori-F (3664 .. 3685) Amp-R (4171 .. 4190) RPS6KA5 gRNA (BRDN0001145521) 8323 bp

Sequence View

TspMIXmaISmaI 60 c c c g g g t g c a a a g a t g g a t a a a g t t t t a a a c a g a g a g g a a t c t t t g c a g c t a a t g g a c c t g g g c c c a c g t t t c t a c c t a t t t c a a a a t t t g t c t c t c c t t a g a a a c g t c g a t t a c c t g g a

120 EF-1 α promoter t c t a g g t c t t g a a a g g a g t g g g a a t t g g c t c c g g t g c c c g t c a g t g g g c a g a g c g c a c a t a g a t c c a g a a c t t t c c t c a c c c t t a a c c g a g g c c a c g g g c a g t c a c c c g t c t c g c g t g t a

180 EF-1 α promoter c g c c c a c a g t c c c c g a g a a g t t g g g g g g a g g g g t c g g c a a t t g a t c c g g t g c c t a g a g a a g c g g g t g t c a g g g g c t c t t c a a c c c c c c t c c c c a g c c g t t a a c t a g g c c a c g g a t c t c t t

240 EF-1 α promoter g g t g g c g c g g g g t a a a c t g g g a a a g t g a t g t c g t g t a c t g g c t c c g c c t t t t t c c c g a g g c c a c c g c g c c c c a t t t g a c c c t t t c a c t a c a g c a c a t g a c c g a g g c g g a a a a a g g g c t c c

300 EF-1 α promoter g t g g g g g a g a a c c g t a t a t a a g t g c a g t a g t c g c c g t g a a c g t t c t t t t t c g c a a c g g g t c a c c c c c t c t t g g c a t a t a t t c a c g t c a t c a g c g g c a c t t g c a a g a a a a a g c g t t g c c c a

360 EF-1 α promoter EF-1 α intron A t t g c c g c c a g a a c a c a g g t a a g t g c c g t g t g t g g t t c c c g c g g g c c t g g c c t c t t t a c g g a a c g g c g g t c t t g t g t c c a t t c a c g g c a c a c a c c a a g g g c g c c c g g a c c g g a g a a a t g c c

420 EF-1 α promoter EF-1 α intron A *KWRAATRSEQD g t t a t g g c c c t t g c g t g c c t t g a a t t a c t t c c a c c t g g c t g c a g t a c g t g a t t c t t g a t c c a a t a c c g g g a a c g c a c g g a a c t t a a t g a a g g t g g a c c g a c g t c a t g c a c t a a g a a c t a g

480 EF-1 α promoter EF-1 α intron A RAEPQFHTPSNSAKRKLLGK c c g a g c t t c g g g t t g g a a g t g g g t g g g a g a g t t c g a g g c c t t g c g c t t a a g g a g c c c c t t g g c t c g a a g c c c a a c c t t c a c c c a c c c t c t c a a g c t c c g g a a c g c g a a t t c c t c g g g g a a

540 EF-1 α promoter EF-1 α intron A AEHKLQPRAQASPGGRAFRT c g c c t c g t g c t t g a g t t g a g g c c t g g c c t g g g c g c t g g g g c c g c c g c g t g c g a a t c t g g t g c g g a g c a c g a a c t c a a c t c c g g a c c g g a c c c g c g a c c c c g g c g g c g c a c g c t t a g a c c a

600 EF-1 α promoter EF-1 α intron A AGERRDRQKRYTELWKFNKI g g c a c c t t c g c g c c t g t c t c g c t g c t t t c g a t a a g t c t c t a g c c a t t t a a a a t t t t t g a t c c g t g g a a g c g c g g a c a g a g c g a c g a a a g c t a t t c a g a g a t c g g t a a a t t t t a a a a a c t a

BspMIBfuAI 660 EF-1 α promoter EF-1 α intron A VQQSAKKRALYDQLHPGLDA g a c c t g c t g c g a c g c t t t t t t t c t g g c a a g a t a g t c t t g t a a a t g c g g g c c a a g a t c t g c c t g g a c g a c g c t g c g a a a a a a a g a c c g t t c t a t c a g a a c a t t t a c g c c c g g t t c t a g a c g

720 EF-1 α promoter EF-1 α intron A CQYKPKQPRPRRRPGHTGAC a c a c t g g t a t t t c g g t t t t t g g g g c c g c g g g c g g c g a c g g g g c c c g t g c g t c c c a g c g c a t g t g a c c a t a a a g c c a a a a a c c c c g g c g c c c g c c g c t g c c c c g g g c a c g c a g g g t c g c g t

780 EF-1 α promoter EF-1 α intron A MNPSAPGALAAVSFRVPTTE c a t g t t c g g c g a g g c g g g g c c t g c g a g c g c g g c c a c c g a g a a t c g g a c g g g g g t a g t c t c g t a c a a g c c g c t c c g c c c c g g a c g c t c g c g c c g g t g g c t c t t a g c c t g c c c c c a t c a g a g

FseI 840 EF-1 α promoter EF-1 α intron A LQGAQEPAQGRAATYRGARP a a g c t g g c c g g c c t g c t c t g g t g c c t g g c c t c g c g c c g c c g t g t a t c g c c c c g c c c t g g g t t c g a c c g g c c g g a c g a g a c c a c g g a c c g g a g c g c g g c g g c a c a t a g c g g g g c g g g a c c c

900 EF-1 α promoter EF-1 α intron A PLAPGTPVLQTLPFIAAERG c g g c a a g g c t g g c c c g g t c g g c a c c a g t t g c g t g a g c g g a a a g a t g g c c g c t t c c c g g c c g c c g t t c c g a c c g g g c c a g c c g t g g t c a a c g c a c t c g c c t t t c t a c c g g c g a a g g g c c g g

960 EF-1 α promoter EF-1 α intron A QQLSSLISSAASPLAPPHTV c t g c t g c a g g g a g c t c a a a a t g g a g g a c g c g g c g c t c g g g a g a g c g g g c g g g t g a g t c a c g a c g a c g t c c c t c g a g t t t t a c c t c c t g c g c c g c g a g c c c t c t c g c c c g c c c a c t c a g t g

1020 EF-1 α promoter EF-1 α intron A WVFSFPRETRLRRKM c c a c a c a a a g g a a a a g g g c c t t t c c g t c c t c a g c c g t c g c t t c a t g t g a c t c c a c g g a g t g g t g t g t t t c c t t t t c c c g g a a a g g c a g g a g t c g g c a g c g a a g t a c a c t g a g g t g c c t c a

1080 EF-1 α promoter EF-1 α intron A a c c g g g c g c c g t c c a g g c a c c t c g a t t a g t t c t c g a g c t t t t g g a g t a c g t c g t c t t t a g t g g c c c g c g g c a g g t c c g t g g a g c t a a t c a a g a g c t c g a a a a c c t c a t g c a g c a g a a a t c

1140 EF-1 α promoter EF-1 α intron A g t t g g g g g g a g g g g t t t t a t g c g a t g g a g t t t c c c c a c a c t g a g t g g g t g g a g a c t g a a g c a a c c c c c c t c c c c a a a a t a c g c t a c c t c a a a g g g g t g t g a c t c a c c c a c c t c t g a c t t c

1200 EF-1 α promoter EF-1 α intron A t t a g g c c a g c t t g g c a c t t g a t g t a a t t c t c c t t g g a a t t t g c c c t t t t t g a g t t t g g a t a a t c c g g t c g a a c c g t g a a c t a c a t t a a g a g g a a c c t t a a a c g g g a a a a a c t c a a a c c t a

1260 EF-1 α promoter EF-1 α intron A T C A A G C C T C A G A C A G T G G T T C EF1a-F c t t g g t t c a t t c t c a a g c c t c a g a c a g t g g t t c a a a g t t t t t t t c t t c c a t t t c a g g t g t g a a c c a a g t a a g a g t t c g g a g t c t g t c a c c a a g t t t c a a a a a a a g a a g g t a a a g t c c a c a

1320 1 5 10 MTEYKPTVRLATRD EF-1 α promoter PuroR T A C T G G C T C A T G T T C G G G T G Puro-R MTEYKPTVRLATRD c g t g a c g t a c g g c c a c c a t g a c c g a g t a c a a g c c c a c g g t g c g c c t c g c c a c c c g c g a c g g c a c t g c a t g c c g g t g g t a c t g g c t c a t g t t c g g g t g c c a c g c g g a g c g g t g g g c g c t g c

Tth111IPflFI 1380 15 20 25 30 DVPRAVRTLAAAFADYPATR PuroR DVPRAVRTLAAAFADYPATR a c g t c c c c a g g g c c g t a c g c a c c c t c g c c g c c g c g t t c g c c g a c t a c c c c g c c a c g c g c c t g c a g g g g t c c c g g c a t g c g t g g g a g c g g c g g c g c a a g c g g c t g a t g g g g c g g t g c g c g g

BstEIIBspEI*RsrII 1440 35 40 45 50 HTVDPDRHIERVTELQELFL PuroR HTVDPDRHIERVTELQELFL a c a c c g t c g a t c c g g a c c g c c a c a t c g a g c g g g t c a c c g a g c t g c a a g a a c t c t t c c t c a t g t g g c a g c t a g g c c t g g c g g t g t a g c t c g c c c a g t g g c t c g a c g t t c t t g a g a a g g a g t

1500 55 60 65 70 TRVGLDIGKVWVADDGAAVA PuroR TRVGLDIGKVWVADDGAAVA c g c g c g t c g g g c t c g a c a t c g g c a a g g t g t g g g t c g c g g a c g a c g g c g c c g c c g t g g c g g g c g c g c a g c c c g a g c t g t a g c c g t t c c a c a c c c a g c g c c t g c t g c c g c g g c g g c a c c g c c

1560 75 80 85 90 VWTTPESVEAGAVFAEIGPR PuroR VWTTPESVEAGAVFAEIGPR t c t g g a c c a c g c c g g a g a g c g t c g a a g c g g g g g c g g t g t t c g c c g a g a t c g g c c c g c g c a a g a c c t g g t g c g g c c t c t c g c a g c t t c g c c c c c g c c a c a a g c g g c t c t a g c c g g g c g c g t

1620 95 100 105 110 MAELSGSRLAAQQQMEGLLA PuroR MAELSGSRLAAQQQMEGLLA t g g c c g a g t t g a g c g g t t c c c g g c t g g c c g c g c a g c a a c a g a t g g a a g g c c t c c t g g c g c a c c g g c t c a a c t c g c c a a g g g c c g a c c g g c g c g t c g t t g t c t a c c t t c c g g a g g a c c g c g

MscI* 1680 115 120 125 130 PHRPKEPAWFLATVGVSPDH PuroR PHRPKEPAWFLATVGVSPDH c g c a c c g g c c c a a g g a g c c c g c g t g g t t c c t g g c c a c c g t c g g a g t c t c g c c c g a c c a c c g c g t g g c c g g g t t c c t c g g g c g c a c c a a g g a c c g g t g g c a g c c t c a g a g c g g g c t g g t g g

1740 135 140 145 150 QGKGLGSAVVLPGVEAAERA PuroR QGKGLGSAVVLPGVEAAERA a g g g c a a g g g t c t g g g c a g c g c c g t c g t g c t c c c c g g a g t g g a g g c g g c c g a g c g c g c c g t c c c g t t c c c a g a c c c g t c g c g g c a g c a c g a g g g g c c t c a c c t c c g c c g g c t c g c g c g g c

1800 155 160 165 170 GVPAFLETSAPRNLPFYERL PuroR G C A A C C T C C C C T T C T A C G A G C Puro-F GVPAFLETSAPRNLPFYERL g g g t g c c c g c c t t c c t g g a g a c c t c c g c g c c c c g c a a c c t c c c c t t c t a c g a g c g g c t c g c c c a c g g g c g g a a g g a c c t c t g g a g g c g c g g g g c g t t g g a g g g g a a g a t g c t c g c c g a g c

SexAI*CsiI 1860 175 180 185 190 GFTVTADVEVPEGPRTWCMT PuroR GFTVTADVEVPEGPRTWCMT g c t t c a c c g t c a c c g c c g a c g t c g a g g t g c c c g a a g g a c c g c g c a c c t g g t g c a t g a c c c c g a a g t g g c a g t g g c g g c t g c a g c t c c a c g g g c t t c c t g g c g c g t g g a c c a c g t a c t g g g

MluI 1920 195 200 RKPGA* PuroR WPRE RKPGA* g c a a g c c c g g t g c c t g a a c g c g t t a a g t c g a c a a t c a a c c t c t g g a t t a c a a a a t t t g t g c g t t c g g g c c a c g g a c t t g c g c a a t t c a g c t g t t a g t t g g a g a c c t a a t g t t t t a a a c a c

1980 WPRE A C A A C G A G G A A A A T G C G A T A C WPRE-R a a a g a t t g a c t g g t a t t c t t a a c t a t g t t g c t c c t t t t a c g c t a t g t g g a t a c g c t g c t t t t t c t a a c t g a c c a t a a g a a t t g a t a c a a c g a g g a a a a t g c g a t a c a c c t a t g c g a c g a a

2040 WPRE MLLLPVWLSFSPPCI MPLYHAIASRMAFIFSSLY t a a t g c c t t t g t a t c a t g c t a t t g c t t c c c g t a t g g c t t t c a t t t t c t c c t c c t t g t a t a a t t a c g g a a a c a t a g t a c g a t a a c g a a g g g c a t a c c g a a a g t a a a a g a g g a g g a a c a t a t

2100 WPRE NPGCCLFMRSCGPLSGNVAW KSWLLSLYEELWPVVRQRGV a a t c c t g g t t g c t g t c t c t t t a t g a g g a g t t g t g g c c c g t t g t c a g g c a a c g t g g c g t g g t t a g g a c c a a c g a c a g a g a a a t a c t c c t c a a c a c c g g g c a a c a g t c c g t t g c a c c g c a c c

PflMI 2160 WPRE CALCLLTQPPLVGALPPPVS VCTVFADATPTGWGIATTCQ t g t g c a c t g t g t t t g c t g a c g c a a c c c c c a c t g g t t g g g g c a t t g c c a c c a c c t g t c a g c a c a c g t g a c a c a a a c g a c t g c g t t g g g g g t g a c c a a c c c c g t a a c g g t g g t g g a c a g t c g

2220 WPRE SFPGLSLSPSLLPRRNSSPP LLSGTFAFPLPIATAELIAA t c c t t t c c g g g a c t t t c g c t t t c c c c c t c c c t a t t g c c a c g g c g g a a c t c a t c g c c g c c t a g g a a a g g c c c t g a a a g c g a a a g g g g g a g g g a t a a c g g t g c c g c c t t g a g t a g c g g c g g a

2280 WPRE ALPAAGQGLGCWALTIPWCC CLARCWTGARLLGTDNSVVL g c c t t g c c c g c t g c t g g a c a g g g g c t c g g c t g t t g g g c a c t g a c a a t t c c g t g g t g t t g t c g g a a c g g g c g a c g a c c t g t c c c c g a g c c g a c a a c c c g t g a c t g t t a a g g c a c c a c a a c a

2340 WPRE (in frame with Factor Xa site) *RGKRPQEGTNGGPNQA RGNHRPFLGCSPVLPPGFCA SGKSSSFPWLLACVATWILR c g g g g a a a t c a t c g t c c t t t c c t t g g c t g c t c g c c t g t g t t g c c a c c t g g a t t c t g c g c g g c c c c t t t a g t a g c a g g a a a g g a a c c g a c g a g c g g a c a c a a c g g t g g a c c t a a g a c g c g c

2400 WPRE (in frame with Factor Xa site) PRGEAVDR 1 RGEI Factor Xa site GRPSATSLRPSIQRTFLPAA GTSFCYVPSALNPADLPSRG g g a c g t c c t t c t g c t a c g t c c c t t c g g c c c t c a a t c c a g c g g a c c t t c c t t c c c g c g g c c c c t g c a g g a a g a c g a t g c a g g g a a g c c g g g a g t t a g g t c g c c t g g a a g g a a g g g c g c c g g

2460 WPRE CCRLCGLFRVFAFALRRVGS LLPALRPLPRLRLRPQTSRI t g c t g c c g g c t c t g c g g c c t c t t c c g c g t c t t c g c c t t c g c c c t c a g a c g a g t c g g a t c t a c g a c g g c c g a g a c g c c g g a g a a g g c g c a g a a g c g g a a g c g g g a g t c t g c t c a g c c t a g a

2520 WPRE PFGPPPRVDFKTNDLQGSCR SLWAASPRRL* c c c t t t g g g c c g c c t c c c c g c g t c g a c t t t a a g a c c a a t g a c t t a c a a g g c a g c t g t a g a g g g a a a c c c g g c g g a g g g g c g c a g c t g a a a t t c t g g t t a c t g a a t g t t c c g t c g a c a t c t

2580 3' LTR ( Δ U3) S* t c t t a g c c a c t t t t t a a a a g a a a a g g g g g g a c t g g a a g g g c t a a t t c a c t c c c a a c g a a g a g a a t c g g t g a a a a a t t t t c t t t t c c c c c c t g a c c t t c c c g a t t a a g t g a g g g t t g c t t c

2640 3' LTR ( Δ U3) a c a a g a t c t g c t t t t t g c t t g t a c t g g g t c t c t c t g g t t a g a c c a g a t c t g a g c c t g g g a t g t t c t a g a c g a a a a a c g a a c a t g a c c c a g a g a g a c c a a t c t g g t c t a g a c t c g g a c c c t

2700 3' LTR ( Δ U3) g c t c t c t g g c t a a c t a g g g a a c c c a c t g c t t a a g c c t c a a t a a a g c t t g c c t t g a g t g c t c g a g a g a c c g a t t g a t c c c t t g g g t g a c g a a t t c g g a g t t a t t t c g a a c g g a a c t c a c g a

2760 3' LTR ( Δ U3) t c a a g t a g t g t g t g c c c g t c t g t t g t g t g a c t c t g g t a a c t a g a g a t c c c t c a g a c c c t t a g t t c a t c a c a c a c g g g c a g a c a a c a c a c t g a g a c c a t t g a t c t c t a g g g a g t c t g g g a a

SnaBI 2820 3' LTR ( Δ U3) t t a g t c a g t g t g g a a a a t c t c t a g c a g t a c g t a t a g t a g t t c a t g t c a t c t t a t t a t t c a a a t c a g t c a c a c c t t t t a g a g a t c g t c a t g c a t a t c a t c a a g t a c a g t a g a a t a a t a a g t

2880 SV40 poly(A) signal g t a t t t a t a a c t t g c a a a g a a a t g a a t a t c a g a g a g t g a g a g g a a c t t g t t t a t t g c a g c c a t a a a t a t t g a a c g t t t c t t t a c t t a t a g t c t c t c a c t c t c c t t g a a c a a a t a a c g t c g

2940 SV40 poly(A) signal C G T T A T C G T A G T G T T T A A A G SV40pA-R t t a t a a t g g t t a c a a a t a a a g c a a t a g c a t c a c a a a t t t c a c a a a t a a a g c a t t t t t t t c a a t a t t a c c a a t g t t t a t t t c g t t a t c g t a g t g t t t a a a g t g t t t a t t t c g t a a a a a a a g

3000 SV40 poly(A) signal G T G G T T T G T C C A A A C T C A T C EBV-rev a c t g c a t t c t a g t t g t g g t t t g t c c a a a c t c a t c a a t g t a t c t t a t c a t g t c t g g c t c t a t g a c g t a a g a t c a a c a c c a a a c a g g t t t g a g t a g t t a c a t a g a a t a g t a c a g a c c g a g a t

3060 SV40 ori g c t a t c c c g c c c c t a a c t c c g c c c a t c c c g c c c c t a a c t c c g c c c a g t t c c g c c c a t t c t c g a t a g g g c g g g g a t t g a g g c g g g t a g g g c g g g g a t t g a g g c g g g t c a a g g c g g g t a a g a

SfiINcoI 3120 SV40 ori c c g c c c c a t g g c t g a c t a a t t t t t t t t a t t t a t g c a g a g g c c g a g g c c g c c t c g g c c t c t g g c g g g g t a c c g a c t g a t t a a a a a a a a t a a a t a c g t c t c c g g c t c c g g c g g a g c c g g a g a

AvrII 3180 SV40 ori G T7 g a g c t a t t c c a g a a g t a g t g a g g a g g c t t t t t t g g a g g c c t a g g g a c g t a c c c a a t t c g c c t c g a t a a g g t c t t c a t c a c t c c t c c g a a a a a a c c t c c g g a t c c c t g c a t g g g t t a a g c g

3240 T7 promoter M13 fwd G G A T A T C A C T C A G C A T A A T T7 T G A C C G G C A G C A A A A T G T M13 Forward G C A A A A T G T T G C A G C A C T G A C C C M13/pUC Forward c c t a t a g t g a g t c g t a t t a c g c g c g c t c a c t g g c c g t c g t t t t a c a a c g t c g t g a c t g g g g g a t a t c a c t c a g c a t a a t g c g c g c g a g t g a c c g g c a g c a a a a t g t t g c a g c a c t g a c c c

3300 a a a a c c c t g g c g t t a c c c a a c t t a a t c g c c t t g c a g c a c a t c c c c c t t t c g c c a g c t g g c t t t t g g g a c c g c a a t g g g t t g a a t t a g c g g a a c g t c g t g t a g g g g g a a a g c g g t c g a c c g

3360 g t a a t a g c g a a g a g g c c c g c a c c g a t c g c c c t t c c c a a c a g t t g c g c a g c c t g a a t g g c g c a t t a t c g c t t c t c c g g g c g t g g c t a g c g g g a a g g g t t g t c a a c g c g t c g g a c t t a c c g c

3420 f1 ori a a t g g g a c g c g c c c t g t a g c g g c g c a t t a a g c g c g g c g g g t g t g g t g g t t a c g c g c a g c g t t a c c c t g c g c g g g a c a t c g c c g c g t a a t t c g c g c c g c c c a c a c c a c c a a t g c g c g t c g c

3480 f1 ori G A G G A A A G C G A A A G A A G G G A F1ori-R t g a c c g c t a c a c t t g c c a g c g c c c t a g c g c c c g c t c c t t t c g c t t t c t t c c c t t c c t t t c a c t g g c g a t g t g a a c g g t c g c g g g a t c g c g g g c g a g g a a a g c g a a a g a a g g g a a g g a a a g

3540 f1 ori t c g c c a c g t t c g c c g g c t t t c c c c g t c a a g c t c t a a a t c g g g g g c t c c c t t t a g g g t t c c a g c g g t g c a a g c g g c c g a a a g g g g c a g t t c g a g a t t t a g c c c c c g a g g g a a a t c c c a a g g

3600 f1 ori g a t t t a g t g c t t t a c g g c a c c t c g a c c c c a a a a a a c t t g a t t a g g g t g a t g g t t c a c g t a c t a a a t c a c g a a a t g c c g t g g a g c t g g g g t t t t t t g a a c t a a t c c c a c t a c c a a g t g c a t

3660 f1 ori g t g g g c c a t c g c c c t g a t a g a c g g t t t t t c g c c c t t t g a c g t t g g a g t c c a c g t t c t t t a c a c c c g g t a g c g g g a c t a t c t g c c a a a a a g c g g g a a a c t g c a a c c t c a g g t g c a a g a a a t

3720 f1 ori G T G G A C T C T T G T T C C A A A C T G G F1ori-F a t a g t g g a c t c t t g t t c c a a a c t g g a a c a a c a c t c a a c c c t a t c t c g g t c t a t t c t t t t g t a t c a c c t g a g a a c a a g g t t t g a c c t t g t t g t g a g t t g g g a t a g a g c c a g a t a a g a a a a c

3780 f1 ori a t t t a t a a g g g a t t t t g c c g a t t t c g g c c t a t t g g t t a a a a a a t g a g c t g a t t t a a c a a a t a a a t a t t c c c t a a a a c g g c t a a a g c c g g a t a a c c a a t t t t t t a c t c g a c t a a a t t g t t t

3840 f1 ori a a t t t a a c g c g a a t t t t a a c a a a a t a t t a a c g c t t a c a a t t t a g g t g g c a c t t t t c g g g g t t a a a t t g c g c t t a a a a t t g t t t t a t a a t t g c g a a t g t t a a a t c c a c c g t g a a a a g c c c c

3900 AmpR promoter a a a t g t g c g c g g a a c c c c t a t t t g t t t a t t t t t c t a a a t a c a t t c a a a t a t g t a t c c g c t t t t a c a c g c g c c t t g g g g a t a a a c a a a t a a a a a g a t t t a t g t a a g t t t a t a c a t a g g c g a

3960 1 signal sequence MSI AmpR promoter AmpR MSI c a t g a g a c a a t a a c c c t g a t a a a t g c t t c a a t a a t a t t g a a a a a g g a a g a g t a t g a g t a t g t a c t c t g t t a t t g g g a c t a t t t a c g a a g t t a t t a t a a c t t t t t c c t t c t c a t a c t c a t a

4020 5 10 15 20 signal sequence QHFRVALIPFFAAFCLPVFA AmpR QHFRVALIPFFAAFCLPVFA t c a a c a t t t c c g t g t c g c c c t t a t t c c c t t t t t t g c g g c a t t t t g c c t t c c t g t t t t t g c a g t t g t a a a g g c a c a g c g g g a a t a a g g g a a a a a a c g c c g t a a a a c g g a a g g a c a a a a a c g

4080 25 30 35 40 HPETLVKVKDAEDQLGARVG AmpR HPETLVKVKDAEDQLGARVG t c a c c c a g a a a c g c t g g t g a a a g t a a a a g a t g c t g a a g a t c a g t t g g g t g c a c g a g t g g g a g t g g g t c t t t g c g a c c a c t t t c a t t t t c t a c g a c t t c t a g t c a a c c c a c g t g c t c a c c c

4140 45 50 55 60 YIELDLNSGKILESFRPEER AmpR YIELDLNSGKILESFRPEER t t a c a t c g a a c t g g a t c t c a a c a g c g g t a a g a t c c t t g a g a g t t t t c g c c c c g a a g a a c g a a t g t a g c t t g a c c t a g a g t t g t c g c c a t t c t a g g a a c t c t c a a a a g c g g g g c t t c t t g c

4200 65 70 75 80 FPMMSTFKVLLCGAVLSRID AmpR C G A T A C A C C G C G C C A T A A T A Amp-R FPMMSTFKVLLCGAVLSRID t t t t c c a a t g a t g a g c a c t t t t a a a g t t c t g c t a t g t g g c g c g g t a t t a t c c c g t a t t g a a a a a g g t t a c t a c t c g t g a a a a t t t c a a g a c g a t a c a c c g c g c c a t a a t a g g g c a t a a c t

ScaI 4260 85 90 95 100 AGQEQLGRRIHYSQNDLVEY AmpR AGQEQLGRRIHYSQNDLVEY c g c c g g g c a a g a g c a a c t c g g t c g c c g c a t a c a c t a t t c t c a g a a t g a c t t g g t t g a g t a g c g g c c c g t t c t c g t t g a g c c a g c g g c g t a t g t g a t a a g a g t c t t a c t g a a c c a a c t c a t

4320 105 110 115 120 SPVTEKHLTDGMTVRELCSA AmpR SPVTEKHLTDGMTVRELCSA c t c a c c a g t c a c a g a a a a g c a t c t t a c g g a t g g c a t g a c a g t a a g a g a a t t a t g c a g t g c g a g t g g t c a g t g t c t t t t c g t a g a a t g c c t a c c g t a c t g t c a t t c t c t t a a t a c g t c a c g

4380 125 130 135 140 AITMSDNTAANLLLTTIGGP AmpR AITMSDNTAANLLLTTIGGP t g c c a t a a c c a t g a g t g a t a a c a c t g c g g c c a a c t t a c t t c t g a c a a c g a t c g g a g g a c c a c g g t a t t g g t a c t c a c t a t t g t g a c g c c g g t t g a a t g a a g a c t g t t g c t a g c c t c c t g g

4440 145 150 155 160 KELTAFLHNMGDHVTRLDRW AmpR KELTAFLHNMGDHVTRLDRW g a a g g a g c t a a c c g c t t t t t t g c a c a a c a t g g g g g a t c a t g t a a c t c g c c t t g a t c g t t g c t t c c t c g a t t g g c g a a a a a a c g t g t t g t a c c c c c t a g t a c a t t g a g c g g a a c t a g c a a c

4500 165 170 175 180 EPELNEAIPNDERDTTMPVA AmpR EPELNEAIPNDERDTTMPVA g g a a c c g g a g c t g a a t g a a g c c a t a c c a a a c g a c g a g c g t g a c a c c a c g a t g c c t g t a g c c c t t g g c c t c g a c t t a c t t c g g t a t g g t t t g c t g c t c g c a c t g t g g t g c t a c g g a c a t c g

4560 185 190 195 200 MATTLRKLLTGELLTLASRQ AmpR MATTLRKLLTGELLTLASRQ a a t g g c a a c a a c g t t g c g c a a a c t a t t a a c t g g c g a a c t a c t t a c t c t a g c t t c c c g g c a t t a c c g t t g t t g c a a c g c g t t t g a t a a t t g a c c g c t t g a t g a a t g a g a t c g a a g g g c c g t

4620 205 210 215 220 QLIDWMEADKVAGPLLRSAL AmpR QLIDWMEADKVAGPLLRSAL a c a a t t a a t a g a c t g g a t g g a g g c g g a t a a a g t t g c a g g a c c a c t t c t g c g c t c g g c c c t t g t t a a t t a t c t g a c c t a c c t c c g c c t a t t t c a a c g t c c t g g t g a a g a c g c g a g c c g g g a

4680 225 230 235 240 PAGWFIADKSGAGERGSRGI AmpR PAGWFIADKSGAGERGSRGI t c c g g c t g g c t g g t t t a t t g c t g a t a a a t c t g g a g c c g g t g a g c g t g g g t c t c g c g g t a t a g g c c g a c c g a c c a a a t a a c g a c t a t t t a g a c c t c g g c c a c t c g c a c c c a g a g c g c c a t a

AhdI 4740 245 250 255 260 IAALGPDGKPSRIVVIYTTG AmpR IAALGPDGKPSRIVVIYTTG c a t t g c a g c a c t g g g g c c a g a t g g t a a g c c c t c c c g t a t c g t a g t t a t c t a c a c g a c g g g g t a a c g t c g t g a c c c c g g t c t a c c a t t c g g g a g g g c a t a g c a t c a a t a g a t g t g c t g c c c

4800 265 270 275 280 SQATMDERNRQIAEIGASLI AmpR SQATMDERNRQIAEIGASLI g a g t c a g g c a a c t a t g g a t g a a c g a a a t a g a c a g a t c g c t g a g a t a g g t g c c t c a c t g a t c t c a g t c c g t t g a t a c c t a c t t g c t t t a t c t g t c t a g c g a c t c t a t c c a c g g a g t g a c t a

4860 285 KHW* AmpR KHW* t a a g c a t t g g t a a c t g t c a g a c c a a g t t t a c t c a t a t a t a c t t t a g a t t g a t t t a a a a c t a t t c g t a a c c a t t g a c a g t c t g g t t c a a a t g a g t a t a t a t g a a a t c t a a c t a a a t t t t g a

4920 t c a t t t t t a a t t t a a a a g g a t c t a g g t g a a g a t c c t t t t t g a t a a t c t c a t g a c c a a a a t a g t a a a a a t t a a a t t t t c c t a g a t c c a c t t c t a g g a a a a a c t a t t a g a g t a c t g g t t t t a

4980 c c c t t a a c g t g a g t t t t c g t t c c a c t g a g c g t c a g a c c c c g t a g a a a a g a t c a a a g g a t c g g g a a t t g c a c t c a a a a g c a a g g t g a c t c g c a g t c t g g g g c a t c t t t t c t a g t t t c c t a g

5040 ori t t c t t g a g a t c c t t t t t t t c t g c g c g t a a t c t g c t g c t t g c a a a c a a a a a a a c c a c c g c t a a g a a c t c t a g g a a a a a a a g a c g c g c a t t a g a c g a c g a a c g t t t g t t t t t t t g g t g g c g a

5100 ori a c c a g c g g t g g t t t g t t t g c c g g a t c a a g a g c t a c c a a c t c t t t t t c c g a a g g t a a c t g g t g g t c g c c a c c a a a c a a a c g g c c t a g t t c t c g a t g g t t g a g a a a a a g g c t t c c a t t g a c c

5160 ori c t t c a g c a g a g c g c a g a t a c c a a a t a c t g t t c t t c t a g t g t a g c c g t a g t t a g g c c a c c a g a a g t c g t c t c g c g t c t a t g g t t t a t g a c a a g a a g a t c a c a t c g g c a t c a a t c c g g t g g t

5220 ori c t t c a a g a a c t c t g t a g c a c c g c c t a c a t a c c t c g c t c t g c t a a t c c t g t t a c c a g t g g c g a a g t t c t t g a g a c a t c g t g g c g g a t g t a t g g a g c g a g a c g a t t a g g a c a a t g g t c a c c g

5280 ori t g c t g c c a g t g g c g a t a a g t c g t g t c t t a c c g g g t t g g a c t c a a g a c g a t a g t t a c c g g a a c g a c g g t c a c c g c t a t t c a g c a c a g a a t g g c c c a a c c t g a g t t c t g c t a t c a a t g g c c t

5340 ori t a a g g c g c a g c g g t c g g g c t g a a c g g g g g g t t c g t g c a c a c a g c c c a g c t t g g a g c g a a c a t t c c g c g t c g c c a g c c c g a c t t g c c c c c c a a g c a c g t g t g t c g g g t c g a a c c t c g c t t g

5400 ori g a c c t a c a c c g a a c t g a g a t a c c t a c a g c g t g a g c t a t g a g a a a g c g c c a c g c t t c c c g a c t g g a t g t g g c t t g a c t c t a t g g a t g t c g c a c t c g a t a c t c t t t c g c g g t g c g a a g g g c t

5460 ori a g g g a g a a a g g c g g a c a g g t a t c c g g t a a g c g g c a g g g t c g g a a c a g g a g a g c g c a c g a g t c c c t c t t t c c g c c t g t c c a t a g g c c a t t c g c c g t c c c a g c c t t g t c c t c t c g c g t g c t c

5520 ori G G G A A A C G C C T G G T A T C T T T pBR322ori-F g g a g c t t c c a g g g g g a a a c g c c t g g t a t c t t t a t a g t c c t g t c g g g t t t c g c c a c c t c t g c c t c g a a g g t c c c c c t t t g c g g a c c a t a g a a a t a t c a g g a c a g c c c a a a g c g g t g g a g a c

5580 ori a c t t g a g c g t c g a t t t t t g t g a t g c t c g t c a g g g g g g c g g a g c c t a t g g a a a a a c g c c a g t g a a c t c g c a g c t a a a a a c a c t a c g a g c a g t c c c c c c g c c t c g g a t a c c t t t t t g c g g t c

5640 c a a c g c g g c c t t t t t a c g g t t c c t g g c c t t t t g c t g g c c t t t t g c t c a c a t g t t c t t t c c g t t g c g c c g g a a a a a t g c c a a g g a c c g g a a a a c g a c c g g a a a a c g a g t g t a c a a g a a a g g

5700 t g c g t t a t c c c c t g a t t c t g t g g a t a a c c g t a t t a c c g c c t t t g a g t g a g c t g a t a c c g c a c g c a a t a g g g g a c t a a g a c a c c t a t t g g c a t a a t g g c g g a a a c t c a c t c g a c t a t g g c g

5760 A G C G A G T C A G T G A G C G A G L4440 t c g c c g c a g c c g a a c g a c c g a g c g c a g c g a g t c a g t g a g c g a g g a a g c g g a a g a g c g c c c a g c g g c g t c g g c t t g c t g g c t c g c g t c g c t c a g t c a c t c g c t c c t t c g c c t t c t c g c g g g

5820 a a t a c g c a a a c c g c c t c t c c c c g c g c g t t g g c c g a t t c a t t a a t g c a g c t g g c a c g a c a g t t a t g c g t t t g g c g g a g a g g g g c g c g c a a c c g g c t a a g t a a t t a c g t c g a c c g t g c t g t c

5880 CAP binding site g t t t c c c g a c t g g a a a g c g g g c a g t g a g c g c a a c g c a a t t a a t g t g a g t t a g c t c a c t c a c a a a g g g c t g a c c t t t c g c c c g t c a c t c g c g t t g c g t t a a t t a c a c t c a a t c g a g t g a g t

5940 -35 -10 CAP binding site lac promoter lac operator A G M13/pUC Reverse t t a g g c a c c c c a g g c t t t a c a c t t t a t g c t t c c g g c t c g t a t g t t g t g t g g a a t t g t g a g a a t c c g t g g g g t c c g a a a t g t g a a a t a c g a a g g c c g a g c a t a c a a c a c a c c t t a a c a c t c

6000 lac operator M13 rev T3 promoter C G G A T A A C A A T T T C A C A C A G G M13/pUC Reverse C A G G A A A C A G C T A T G A C M13 Reverse G C A A T T A A T3 c g g a t a a c a a t t t c a c a c a g g a a a c a g c t a t g a c c a t g a t t a c g c c a a g c g c g c a a t t a a g c c t a t t g t t a a a g t g t g t c c t t t g t c g a t a c t g g t a c t a a t g c g g t t c g c g c g t t a a t t

6060 T3 promoter RSV promoter C C C T C A C T A A A G G T3 c c c t c a c t a a a g g g a a c a a a a g c t g g a g c t g c a a g c t t a a t g t a g t c t t a t g c a a t a c t c g g g a g t g a t t t c c c t t g t t t t c g a c c t c g a c g t t c g a a t t a c a t c a g a a t a c g t t a t g a g

6120 RSV promoter t t g t a g t c t t g c a a c a t g g t a a c g a t g a g t t a g c a a c a t g c c t t a c a a g g a g a g a a a a a g a a c a t c a g a a c g t t g t a c c a t t g c t a c t c a a t c g t t g t a c g g a a t g t t c c t c t c t t t t t c

SphI 6180 RSV promoter c a c c g t g c a t g c c g a t t g g t g g a a g t a a g g t g g t a c g a t c g t g c c t t a t t a g g a a g g c a a g t g g c a c g t a c g g c t a a c c a c c t t c a t t c c a c c a t g c t a g c a c g g a a t a a t c c t t c c g t t

6240 RSV promoter c a g a c g g g t c t g a c a t g g a t t g g a c g a a c c a c t g a a t t g c c g c a t t g c a g a g a t a t t g t a g t c t g c c c a g a c t g t a c c t a a c c t g c t t g g t g a c t t a a c g g c g t a a c g t c t c t a t a a c a t

6300 RSV promoter 5' LTR (truncated) t t t a a g t g c c t a g c t c g a t a c a t a a a c g g g t c t c t c t g g t t a g a c c a g a t c t g a g c c t g g a a a t t c a c g g a t c g a g c t a t g t a t t t g c c c a g a g a g a c c a a t c t g g t c t a g a c t c g g a c c

6360 5' LTR (truncated) g a g c t c t c t g g c t a a c t a g g g a a c c c a c t g c t t a a g c c t c a a t a a a g c t t g c c t t g a g t g c t c g a g a g a c c g a t t g a t c c c t t g g g t g a c g a a t t c g g a g t t a t t t c g a a c g g a a c t c a c

6420 5' LTR (truncated) c t t c a a g t a g t g t g t g c c c g t c t g t t g t g t g a c t c t g g t a a c t a g a g a t c c c t c a g a c c c g a a g t t c a t c a c a c a c g g g c a g a c a a c a c a c t g a g a c c a t t g a t c t c t a g g g a g t c t g g g

6480 5' LTR (truncated) t t t t a g t c a g t g t g g a a a a t c t c t a g c a g t g g c g c c c g a a c a g g g a c t t g a a a g c g a a a g a a a a t c a g t c a c a c c t t t t a g a g a t c g t c a c c g c g g g c t t g t c c c t g a a c t t t c g c t t t c

6540 HIV-1 Ψ g g a a a c c a g a g g a g c t c t c t c g a c g c a g g a c t c g g c t t g c t g a a g c g c g c a c g g c a a g a g c c t t t g g t c t c c t c g a g a g a g c t g c g t c c t g a g c c g a a c g a c t t c g c g c g t g c c g t t c t c

6600 HIV-1 Ψ g c g a g g g g c g g c g a c t g g t g a g t a c g c c a a a a a t t t t g a c t a g c g g a g g c t a g a a g g a g a c g c t c c c c g c c g c t g a c c a c t c a t g c g g t t t t t a a a a c t g a t c g c c t c c g a t c t t c c t c t

NruI* 6660 HIV-1 Ψ g a g a t g g g t g c g a g a g c g t c a g t a t t a a g c g g g g g a g a a t t a g a t c g c g a t g g g a a a a a a c t c t a c c c a c g c t c t c g c a g t c a t a a t t c g c c c c c t c t t a a t c t a g c g c t a c c c t t t t t t

6720 t t c g g t t a a g g c c a g g g g g a a a g a a a a a a t a t a a a t t a a a a c a t a t a g t a t g g g c a a g c a a a g c c a a t t c c g g t c c c c c t t t c t t t t t t a t a t t t a a t t t t g t a t a t c a t a c c c g t t c g t

6780 g g g a g c t a g a a c g a t t c g c a g t t a a t c c t g g c c t g t t a g a a a c a t c a g a a g g c t g t a g a c c c c t c g a t c t t g c t a a g c g t c a a t t a g g a c c g g a c a a t c t t t g t a g t c t t c c g a c a t c t g

6840 a a a t a c t g g g a c a g c t a c a a c c a t c c c t t c a g a c a g g a t c a g a a g a a c t t a g a t c a t t a t t t t a t g a c c c t g t c g a t g t t g g t a g g g a a g t c t g t c c t a g t c t t c t t g a a t c t a g t a a t a

6900 a t a a t a c a g t a g c a a c c c t c t a t t g t g t g c a t c a a a g g a t a g a g a t a a a a g a c a c c a a g g t a t t a t g t c a t c g t t g g g a g a t a a c a c a c g t a g t t t c c t a t c t c t a t t t t c t g t g g t t c c

NotI 6960 a a g c t t t a g a c a a g a t a g a g g a a g a g c a a a a c a a a a g t a a g a c c a c c g c a c a g c a a g c g g t t c g a a a t c t g t t c t a t c t c c t t c t c g t t t t g t t t t c a t t c t g g t g g c g t g t c g t t c g c c

7020 MRDNWRSELY c c g c t g a t c t t c a g a c c t g g a g g a g g a g a t a t g a g g g a c a a t t g g a g a a g t g a a t t a t a t g g c g a c t a g a a g t c t g g a c c t c c t c c t c t a t a c t c c c t g t t a a c c t c t t c a c t t a a t a t a

7080 KYKVVKIEPLGVAPTKAKRR a a a t a t a a a g t a g t a a a a a t t g a a c c a t t a g g a g t a g c a c c c a c c a a g g c a a a g a g a a g a t t t a t a t t t c a t c a t t t t t a a c t t g g t a a t c c t c a t c g t g g g t g g t t c c g t t t c t c t t c t

7140 RRE VVQREKRAVGIGALFLGFLG g t g g t g c a g a g a g a a a a a a g a g c a g t g g g a a t a g g a g c t t t g t t c c t t g g g t t c t t g g g a c a c c a c g t c t c t c t t t t t t c t c g t c a c c c t t a t c c t c g a a a c a a g g a a c c c a a g a a c c c t

7200 RRE AAGSTMGAASMTLTVQARQL g c a g c a g g a a g c a c t a t g g g c g c a g c g t c a a t g a c g c t g a c g g t a c a g g c c a g a c a a t t a c g t c g t c c t t c g t g a t a c c c g c g t c g c a g t t a c t g c g a c t g c c a t g t c c g g t c t g t t a a t

7260 RRE LSGIVQQQNNLLRAIEAQQH t t g t c t g g t a t a g t g c a g c a g c a g a a c a a t t t g c t g a g g g c t a t t g a g g c g c a a c a g c a t a a c a g a c c a t a t c a c g t c g t c g t c t t g t t a a a c g a c t c c c g a t a a c t c c g c g t t g t c g t a

7320 RRE LLQLTVWGIKQLQARILAVE c t g t t g c a a c t c a c a g t c t g g g g c a t c a a g c a g c t c c a g g c a a g a a t c c t g g c t g t g g a a g a c a a c g t t g a g t g t c a g a c c c c g t a g t t c g t c g a g g t c c g t t c t t a g g a c c g a c a c c t t

7380 RRE RYLKDQQLLGIWGCSGKLIC a g a t a c c t a a a g g a t c a a c a g c t c c t g g g g a t t t g g g g t t g c t c t g g a a a a c t c a t t t g c t c t a t g g a t t t c c t a g t t g t c g a g g a c c c c t a a a c c c c a a c g a g a c c t t t t g a g t a a a c g

AleI 7440 TTAVPWNASWSNKSLEQIWN a c c a c t g c t g t g c c t t g g a a t g c t a g t t g g a g t a a t a a a t c t c t g g a a c a g a t t t g g a a t t g g t g a c g a c a c g g a a c c t t a c g a t c a a c c t c a t t a t t t a g a g a c c t t g t c t a a a c c t t a

7500 HTTWMEWDREINNYTSLIHS c a c a c g a c c t g g a t g g a g t g g g a c a g a g a a a t t a a c a a t t a c a c a a g c t t a a t a c a c t c c g t g t g c t g g a c c t a c c t c a c c c t g t c t c t t t a a t t g t t a a t g t g t t c g a a t t a t g t g a g g

7560 1 5 10 KNEQELLELD gp41 peptide LIEESQNQQEKNEQELLELD t t a a t t g a a g a a t c g c a a a a c c a g c a a g a a a a g a a t g a a c a a g a a t t a t t g g a a t t a g a t a a t t a a c t t c t t a g c g t t t t g g t c g t t c t t t t c t t a c t t g t t c t t a a t a a c c t t a a t c t a

7620 15 KWASL (in frame with gp41 peptide) WNWFNITNWLWYIKL gp41 peptide KWASLWNWFNITNWLWYIKL a a a t g g g c a a g t t t g t g g a a t t g g t t t a a c a t a a c a a a t t g g c t g t g g t a t a t a a a a t t a t t t a c c c g t t c a a a c a c c t t a a c c a a a t t g t a t t g t t t a a c c g a c a c c a t a t a t t t t a a t

7680 (in frame with gp41 peptide) FIMIVGGLVGLRIVFAVLSI FIMIVGGLVGLRIVFAVLSI t t c a t a a t g a t a g t a g g a g g c t t g g t a g g t t t a a g a a t a g t t t t t g c t g t a c t t t c t a t a a a g t a t t a c t a t c a t c c t c c g a a c c a t c c a a a t t c t t a t c a a a a a c g a c a t g a a a g a t a t

7740 (in frame with gp41 peptide) VNRVRQGYSPLSFQTHLPTP VNRVRQGYSPLSFQTHLPTP g t g a a t a g a g t t a g g c a g g g a t a t t c a c c a t t a t c g t t t c a g a c c c a c c t c c c a a c c c c g c a c t t a t c t c a a t c c g t c c c t a t a a g t g g t a a t a g c a a a g t c t g g g t g g a g g g t t g g g g c

PpuMIKflI 7800 (in frame with gp41 peptide) RGPREGLFPMIPSYLHIRYK U6 promoter G A G G G C C T A T T T C C C A T G A T T hU6-F RGPREGLFPMIPSYLHIRYK a g g g g a c c c a g a g a g g g c c t a t t t c c c a t g a t t c c t t c a t a t t t g c a t a t a c g a t a c a a g t c c c c t g g g t c t c t c c c g g a t a a a g g g t a c t a a g g a a g t a t a a a c g t a t a t g c t a t g t t c

7860 (in frame with gp41 peptide) AVREIIRINLTVNTKILVQN U6 promoter AVREIIRINLTVNTKILVQN g c t g t t a g a g a g a t a a t t a g a a t t a a t t t g a c t g t a a a c a c a a a g a t a t t a g t a c a a a a t c g a c a a t c t c t c t a t t a a t c t t a a t t a a a c t g a c a t t t g t g t t t c t a t a a t c a t g t t t t a

7920 T* U6 promoter T* a c g t g a c g t a g a a a g t a a t a a t t t c t t g g g t a g t t t g c a g t t t t a a a a t t a t g t t t t a a a t g c a c t g c a t c t t t c a t t a t t a a a g a a c c c a t c a a a c g t c a a a a t t t t a a t a c a a a a t t t

NdeI 7980 U6 promoter G A C T A T C A T A T G C T T A C C G T LKO.1 5' a t g g a c t a t c a t a t g c t t a c c g t a a c t t g a a a g t a t t t c g a t t t c t t g g c t t t a t a t a t c t a c c t g a t a g t a t a c g a a t g g c a t t g a a c t t t c a t a a a g c t a a a g a a c c g a a a t a t a t a g

8040 U6 promoter gRNA scaffold t t g t g g a a a g g a c g a a a c a c c g g g c a c c a g a t a t t g t c a g a g g t t t t a g a g c t a g a a a t a a a c a c c t t t c c t g c t t t g t g g c c c g t g g t c t a t a a c a g t c t c c a a a a t c t c g a t c t t t a t

8100 gRNA scaffold g c a a g t t a a a a t a a g g c t a g t c c g t t a t c a a c t t g a a a a a g t g g c a c c g a g t c g g t g c t t c g t t c a a t t t t a t t c c g a t c a g g c a a t a g t t g a a c t t t t t c a c c g t g g c t c a g c c a c g a a

8160 cPPT/CTS t t t t a a g c t t g g c g t a a c t a g a t c t t g a g a c a a a t g g c a g t a t t c a t c c a c a a t t t t a a a a a a a t t c g a a c c g c a t t g a t c t a g a a c t c t g t t t a c c g t c a t a a g t a g g t g t t a a a a t t t

8220 cPPT/CTS a g a a a a g g g g g g a t t g g g g g g t a c a g t g c a g g g g a a a g a a t a g t a g a c a t a a t a g c a a c a t c t t t t c c c c c c t a a c c c c c c a t g t c a c g t c c c c t t t c t t a t c a t c t g t a t t a t c g t t g t

8280 cPPT/CTS g a c a t a c a a a c t a a a g a a t t a c a a a a a c a a a t t a c a a a a a t t c a a a a t t t t c g g g t t t a t c t g t a t g t t t g a t t t c t t a a t g t t t t t g t t t a a t g t t t t t a a g t t t t a a a a g c c c a a a t a

PspXI 8323 t a c a g g g a c a g c a g a g a t c c a c t t t g g c g c c g g c t c g a g g g g g a t g t c c c t g t c g t c t c t a g g t g a a a c c g c g g c c g a g c t c c c c c

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.