Addgene Carl-ReNL-KDEL_pcDNA3 Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: Carl-ReNL-KDEL_pcDNA3 Sequencing Result


Map View

pRS-marker (7369 .. 7388) BglII (7337) SgrDI (7323) SspI (7206) Amp-R (6952 .. 6971) ScaI (6882) PvuI (6772) pBR322ori-F (5650 .. 5669) PciI (5509) L4440 (5399 .. 5416) CAP binding site M13/pUC Reverse (5181 .. 5203) lac operator M13 Reverse (5168 .. 5184) BstZ17I (5130) EBV-rev (5089 .. 5108) SV40pA-R (5035 .. 5054) PfoI (4933) BstBI (4840) Neo-F (4694 .. 4713) RsrII (4674) BssHII (4555) Neo-R (4084 .. 4103) SmaI (3971) TspMI - XmaI (3969) AvrII (3948) SV40pro-F (3876 .. 3895) MfeI (80) NruI (127) MluI (147) SpeI (168) CMV enhancer NdeI (403) SnaBI (509) CMV-F (688 .. 708) T7 (782 .. 801) HindIII (808) SacII (872) BamHI (873) EcoNI (2344) BsgI (2536) KflI - PpuMI (2663) Acc65I (2797) KpnI (2801) EcoRI (2818) EcoRV (2830) PaeR7I - PspXI - XhoI (2851) XbaI (2863) PspOMI (2869) ApaI (2873) SP6 (2879 .. 2896) BGH-rev (2916 .. 2933) F1ori-R (3279 .. 3298) DraIII (3425) F1ori-F (3489 .. 3510) pBABE 3' (3629 .. 3649) Carl-ReNL-KDEL_pcDNA3 7406 bp

Sequence View

5ʹ 3ʹ 60 t g c t c c c t g c t t g t g t g t t g g a g g t c g c t g a g t a g t g c g c g a g c a a a a t t t a a g c t a c a a a c g a g g g a c g a a c a c a c a a c c t c c a g c g a c t c a t c a c g c g c t c g t t t t a a a t t c g a t g t t

MfeI 120 c a a g g c a a g g c t t g a c c g a c a a t t g c a t g a a g a a t c t g c t t a g g g t t a g g c g t t t t g c g c g t t c c g t t c c g a a c t g g c t g t t a a c g t a c t t c t t a g a c g a a t c c c a a t c c g c a a a a c g c g

SpeIMluINruI 180 CMV enhancer t g c t t c g c g a t g t a c g g g c c a g a t a t a c g c g t t g a c a t t g a t t a t t g a c t a g t t a t t a a t a c g a a g c g c t a c a t g c c c g g t c t a t a t g c g c a a c t g t a a c t a a t a a c t g a t c a a t a a t t a

240 CMV enhancer a g t a a t c a a t t a c g g g g t c a t t a g t t c a t a g c c c a t a t a t g g a g t t c c g c g t t a c a t a a c t c a t t a g t t a a t g c c c c a g t a a t c a a g t a t c g g g t a t a t a c c t c a a g g c g c a a t g t a t t g

300 CMV enhancer t t a c g g t a a a t g g c c c g c c t g g c t g a c c g c c c a a c g a c c c c c g c c c a t t g a c g t c a a t a a a a t g c c a t t t a c c g g g c g g a c c g a c t g g c g g g t t g c t g g g g g c g g g t a a c t g c a g t t a t t

360 CMV enhancer t g a c g t a t g t t c c c a t a g t a a c g c c a a t a g g g a c t t t c c a t t g a c g t c a a t g g g t g g a g t a c t g c a t a c a a g g g t a t c a t t g c g g t t a t c c c t g a a a g g t a a c t g c a g t t a c c c a c c t c a

NdeI 420 CMV enhancer a t t t a c g g t a a a c t g c c c a c t t g g c a g t a c a t c a a g t g t a t c a t a t g c c a a g t a c g c c c c t a a a t g c c a t t t g a c g g g t g a a c c g t c a t g t a g t t c a c a t a g t a t a c g g t t c a t g c g g g g

480 CMV enhancer c t a t t g a c g t c a a t g a c g g t a a a t g g c c c g c c t g g c a t t a t g c c c a g t a c a t g a c c t t a t g a t a a c t g c a g t t a c t g c c a t t t a c c g g g c g g a c c g t a a t a c g g g t c a t g t a c t g g a a t a

SnaBI 540 CMV enhancer CMV promoter g g g a c t t t c c t a c t t g g c a g t a c a t c t a c g t a t t a g t c a t c g c t a t t a c c a t g g t g a t g c c c c t g a a a g g a t g a a c c g t c a t g t a g a t g c a t a a t c a g t a g c g a t a a t g g t a c c a c t a c g

600 CMV promoter g g t t t t g g c a g t a c a t c a a t g g g c g t g g a t a g c g g t t t g a c t c a c g g g g a t t t c c a a g t c c c a a a a c c g t c a t g t a g t t a c c c g c a c c t a t c g c c a a a c t g a g t g c c c c t a a a g g t t c a g

660 CMV promoter t c c a c c c c a t t g a c g t c a a t g g g a g t t t g t t t t g g c a c c a a a a t c a a c g g g a c t t t c c a a a g g t g g g g t a a c t g c a g t t a c c c t c a a a c a a a a c c g t g g t t t t a g t t g c c c t g a a a g g t t

720 CMV promoter C G C A A A T G G G C G G T A G G C G T G CMV-F MGGRRVRWE a a t g t c g t a a c a a c t c c g c c c c a t t g a c g c a a a t g g g c g g t a g g c g t g t a c g g t g g g a g g t t a c a g c a t t g t t g a g g c g g g g t a a c t g c g t t t a c c c g c c a t c c g c a c a t g c c a c c c t c c

780 CMV promoter VYISRALWLTREPTAYWLIE t c t a t a t a a g c a g a g c t c t c t g g c t a a c t a g a g a a c c c a c t g c t t a c t g g c t t a t c g a a a a g a t a t a t t c g t c t c g a g a g a c c g a t t g a t c t c t t g g g t g a c g a a t g a c c g a a t a g c t t t

HindIII 840 T7 promoter T A A T A C G A C T C A C T A T A G G G T7 MLLPV INTTHYRETQACGRHHAAAR *YSESYPSGLKRGGGHQQGD t t a a t a c g a c t c a c t a t a g g g a g a c c c a a g c t t g c g g c c g c c a c c a t g c t g c t g c c c g t c a a t t a t g c t g a g t g a t a t c c c t c t g g g t t c g a a c g c c g g c g g t g g t a c g a c g a c g g g c a g

SacIIBamHI 900 PLLLGLLGAAADPMVSKGEE PPAAGPAGRRRGSHGEQGRG GQQQAQQAGGRIGHHALALL c c c c t g c t g c t g g g c c t g c t g g g c g c c g c c g c g g a t c c c a t g g t g a g c a a g g g c g a g g a g g g g g a c g a c g a c c c g g a c g a c c c g c g g c g g c g c c t a g g g t a c c a c t c g t t c c c g c t c c t c

960 VIKEFMRFKVRMEGSMNGHE GHQRVHALQGAHGGLHERPR DDFLEHAELHAHLAGHVAVL g t c a t c a a a g a g t t c a t g c g c t t c a a g g t g c g c a t g g a g g g c t c c a t g a a c g g c c a c g a g c a g t a g t t t c t c a a g t a c g c g a a g t t c c a c g c g t a c c t c c c g a g g t a c t t g c c g g t g c t c

1020 FEIEGEGEGRPYEGTQTAKL VRDRGRGRGPPLRGHPDRQA ELDLALALAAGVLAGLGGLQ t t c g a g a t c g a g g g c g a g g g c g a g g g c c g c c c c t a c g a g g g c a c c c a g a c c g c c a a g c t g a a g c t c t a g c t c c c g c t c c c g c t c c c g g c g g g g a t g c t c c c g t g g g t c t g g c g g t t c g a c

1080 KVTKGGPLPFAWDILSPQFM EGDQGRPPALRLGHPVPPVH LHGLAAGQGEGPVDQGGLEH a a g g t g a c c a a g g g c g g c c c c c t g c c c t t c g c c t g g g a c a t c c t g t c c c c c c a g t t c a t g t t c c a c t g g t t c c c g c c g g g g g a c g g g a a g c g g a c c c t g t a g g a c a g g g g g g t c a a g t a c

1140 YGSKAYVKHPADIPDYKKLS VRLQGVREAPRRHPRLQEAV VAGLRVHLVGGVDGIVLLQG t a c g g c t c c a a g g c g t a c g t g a a g c a c c c c g c c g a c a t c c c c g a t t a c a a g a a g c t g t c c a t g c c g a g g t t c c g c a t g c a c t t c g t g g g g c g g c t g t a g g g g c t a a t g t t c t t c g a c a g g

1200 FPEGFKWERVMNFEDGGLVT LPRGLQVGARDELRGRRSGD EGLAELPLAHHVELVATQHG t t c c c c g a g g g c t t c a a g t g g g a g c g c g t g a t g a a c t t c g a g g a c g g c g g t c t g g t g a c c a a g g g g c t c c c g a a g t t c a c c c t c g c g c a c t a c t t g a a g c t c c t g c c g c c a g a c c a c t g g

1260 VTQDSSLQDGTLIYKVKMRG RDPGLLPAGRHADLQGEDAR HGLVGGQLVARQDVLHLHAA g t g a c c c a g g a c t c c t c c c t g c a g g a c g g c a c g c t g a t c t a c a a g g t g a a g a t g c g c g g c c a c t g g g t c c t g a g g a g g g a c g t c c t g c c g t g c g a c t a g a t g t t c c a c t t c t a c g c g c c g

1320 TNFPPDGPVMQKKTMGWEAS HQLPPRRPRNAEEDHGLGGL GVEGGVAGYHLLLGHAPLGG a c c a a c t t c c c c c c c g a c g g c c c c g t a a t g c a g a a g a a g a c c a t g g g c t g g g a g g c c t c c t g g t t g a a g g g g g g g c t g c c g g g g c a t t a c g t c t t c t t c t g g t a c c c g a c c c t c c g g a g g

1380 TERLYPRDGVLKGEIHQALK HRAPVPPRRRAEGRDPPGPE GLAQVGAVAHQLALDVLGQL a c c g a g c g c c t g t a c c c c c g c g a c g g c g t g c t g a a g g g c g a g a t c c a c c a g g c c c t g a a g t g g c t c g c g g a c a t g g g g g c g c t g c c g c a c g a c t t c c c g c t c t a g g t g g t c c g g g a c t t c

1440 LKDGGHYLVEFKTIYMAKKP AEGRRPLPGGVQDHLHGQEA QLVAAVVQHLELGDVHGLLG c t g a a g g a c g g c g g c c a c t a c c t g g t g g a g t t c a a g a c c a t c t a c a t g g c c a a g a a g c c c g a c t t c c t g c c g c c g g t g a t g g a c c a c c t c a a g t t c t g g t a g a t g t a c c g g t t c t t c g g g

1500 VQLPGYYYVDTKLDITSHNE RATARLLLRGHQAGHHLPQR HLQGAVVVHVGLQVDGGVVL g t g c a a c t g c c c g g c t a c t a c t a c g t g g a c a c c a a g c t g g a c a t c a c c t c c c a c a a c g a g c a c g t t g a c g g g c c g a t g a t g a t g c a c c t g t g g t t c g a c c t g t a g t g g a g g g t g t t g c t c

1560 DYTIVEQYERSEGRHHLFLG GLHHRGTVRALRGPPPPVPG VVGDHFLVLAGLAAVVQEQP g a c t a c a c c a t c g t g g a a c a g t a c g a g c g c t c c g a g g g c c g c c a c c a c c t g t t c c t g g g g c t g a t g t g g t a g c a c c t t g t c a t g c t c g c g a g g c t c c c g g c g g t g g t g g a c a a g g a c c c c

1620 HGTGSTGSGSSGTASSEDNN AWHRQHRQRQLRHRLLRGQQ MAGAAGAAAAGAGGGGLVVV c a t g g c a c c g g c a g c a c c g g c a g c g g c a g c t c c g g c a c c g c c t c c t c c g a g g a c a a c a a c g t a c c g t g g c c g t c g t g g c c g t c g c c g t c g a g g c c g t g g c g g a g g a g g c t c c t g t t g t t g

1680 MAVIKEFMRFKVRMEGSMNG HGRHQRVHALQGAHGGLHER HGDDFLEHAELHAHLAGHVA a t g g c c g t c a t c a a a g a g t t c a t g c g c t t c a a g g t g c g c a t g g a g g g c t c c a t g a a c g g c t a c c g g c a g t a g t t t c t c a a g t a c g c g a a g t t c c a c g c g t a c c t c c c g a g g t a c t t g c c g

1740 HEFEIEGEGEGRPYEGTQTA PRVRDRGRGRGPPLRGHPDR VLELDLALALAAGVLAGLGG c a c g a g t t c g a g a t c g a g g g c g a g g g c g a g g g c c g c c c c t a c g a g g g c a c c c a g a c c g c c g t g c t c a a g c t c t a g c t c c c g c t c c c g c t c c c g g c g g g g a t g c t c c c g t g g g t c t g g c g g

1800 KLKVTKGGPLPFAWDILSPQ QAEGDQGRPPALRLGHPVPP LQLHGLAAGQGEGPVDQGGL a a g c t g a a g g t g a c c a a g g g c g g c c c c c t g c c c t t c g c c t g g g a c a t c c t g t c c c c c c a g t t c g a c t t c c a c t g g t t c c c g c c g g g g g a c g g g a a g c g g a c c c t g t a g g a c a g g g g g g t c

1860 FMYGSKAYVKHPADIPDYKK VHVRLQGVREAPRRHPRLQE EHVAGLRVHLVGGVDGIVLL t t c a t g t a c g g c t c c a a g g c g t a c g t g a a g c a c c c c g c c g a c a t c c c c g a t t a c a a g a a g a a g t a c a t g c c g a g g t t c c g c a t g c a c t t c g t g g g g c g g c t g t a g g g g c t a a t g t t c t t c

1920 LSFPEGFKWERVMNFEDGGL AVLPRGLQVGARDELRGRRS QGEGLAELPLAHHVELVATQ c t g t c c t t c c c c g a g g g c t t c a a g t g g g a g c g c g t g a t g a a c t t c g a g g a c g g c g g t c t g g a c a g g a a g g g g c t c c c g a a g t t c a c c c t c g c g c a c t a c t t g a a g c t c c t g c c g c c a g a c

1980 VTVTQDSSLQDGTLIYKVKM GDRDPGLLPAGRHADLQGED HGHGLVGGQLVARQDVLHLH g t g a c c g t g a c c c a g g a c t c c t c c c t g c a g g a c g g c a c g c t g a t c t a c a a g g t g a a g a t g c a c t g g c a c t g g g t c c t g a g g a g g g a c g t c c t g c c g t g c g a c t a g a t g t t c c a c t t c t a c

2040 RGTNFPPDGPVMQKKTMGWE ARHQLPPRRPRNAEEDHGLG AAGVEGGVAGYHLLLGHAPL c g c g g c a c c a a c t t c c c c c c c g a c g g c c c c g t a a t g c a g a a g a a g a c c a t g g g c t g g g a g g c g c c g t g g t t g a a g g g g g g g c t g c c g g g g c a t t a c g t c t t c t t c t g g t a c c c g a c c c t c

2100 ASTERLYPRDGVLKGEIHQA GLHRAPVPPRRRAEGRDPPG GGGLAQVGAVAHQLALDVLG g c c t c c a c c g a g c g c c t g t a c c c c c g c g a c g g c g t g c t g a a g g g c g a g a t c c a c c a g g c c c g g a g g t g g c t c g c g g a c a t g g g g g c g c t g c c g c a c g a c t t c c c g c t c t a g g t g g t c c g g

2160 LKLKDGGHYLVEFKTIYMAK PEAEGRRPLPGGVQDHLHGQ QLQLVAAVVQHLELGDVHGL c t g a a g c t g a a g g a c g g c g g c c a c t a c c t g g t g g a g t t c a a g a c c a t c t a c a t g g c c a a g g a c t t c g a c t t c c t g c c g c c g g t g a t g g a c c a c c t c a a g t t c t g g t a g a t g t a c c g g t t c

2220 KPVQLPGYYYVDTKLDITSH EARATARLLLRGHQAGHHLP LGHLQGAVVVHVGLQVDGGV a a g c c c g t g c a a c t g c c c g g c t a c t a c t a c g t g g a c a c c a a g c t g g a c a t c a c c t c c c a c t t c g g g c a c g t t g a c g g g c c g a t g a t g a t g c a c c t g t g g t t c g a c c t g t a g t g g a g g g t g

2280 NEDYTIVEQYERSEGRHHLF QRGLHHRGTVRALRGPPPPV VLVVGDHFLVLAGLAAVVQE a a c g a g g a c t a c a c c a t c g t g g a a c a g t a c g a g c g c t c c g a g g g c c g c c a c c a c c t g t t c t t g c t c c t g a t g t g g t a g c a c c t t g t c a t g c t c g c g a g g c t c c c g g c g g t g g t g g a c a a g

2340 RLEDFVGDWRQTAGYNLDQV PAGRFRWGLATDSRLQPGPS PQFIENPVPSLCGAVVQVLD c g g c t g g a a g a t t t c g t t g g g g a c t g g c g a c a g a c a g c c g g c t a c a a c c t g g a c c a a g t c g c c g a c c t t c t a a a g c a a c c c c t g a c c g c t g t c t g t c g g c c g a t g t t g g a c c t g g t t c a g

EcoNI 2400 LEQGGVSSLFQNLGVSVTPI P* KFLSTHGTQKLIEPHGYSRD c t t g a a c a g g g a g g t g t g t c c a g t t t g t t t c a g a a t c t c g g g g t g t c c g t a a c t c c g a t c g a a c t t g t c c c t c c a c a c a g g t c a a a c a a a g t c t t a g a g c c c c a c a g g c a t t g a g g c t a g

2460 QRIVLSGENGLKIDIHVIIP LPNDQATFIPQLDVDMDDDR c a a a g g a t t g t c c t g a g c g g t g a a a a t g g g c t g a a g a t c g a c a t c c a t g t c a t c a t c c c g g t t t c c t a a c a g g a c t c g c c a c t t t t a c c c g a c t t c t a g c t g t a g g t a c a g t a g t a g g g c

2520 YEGLSGDQMGQIEKIFKVVY IFTQAAVLHALDFFNKLHHV t a t g a a g g t c t g a g c g g c g a c c a a a t g g g c c a g a t c g a a a a a a t t t t t a a g g t g g t g t a c a t a c t t c c a g a c t c g c c g c t g g t t t a c c c g g t c t a g c t t t t t t a a a a a t t c c a c c a c a t g

BsgI 2580 PVDDHHFKVILHYGTLVIDG RHIIM c c t g t g g a t g a t c a t c a c t t t a a g g t g a t c c t g c a c t a t g g c a c a c t g g t a a t c g a c g g g g g a c a c c t a c t a g t a g t g a a a t t c c a c t a g g a c g t g a t a c c g t g t g a c c a t t a g c t g c c c

2640 VTPNMIDYFGRPYEGIAVFD g t t a c g c c g a a c a t g a t c g a c t a t t t c g g a c g g c c g t a t g a a g g c a t c g c c g t g t t c g a c c a a t g c g g c t t g t a c t a g c t g a t a a a g c c t g c c g g c a t a c t t c c g t a g c g g c a c a a g c t g

PpuMIKflI 2700 GKKITVTGTLWNGNKIIDER g g c a a a a a g a t c a c t g t a a c a g g g a c c c t g t g g a a c g g c a a c a a a a t t a t c g a c g a g c g c c c g t t t t t c t a g t g a c a t t g t c c c t g g g a c a c c t t g c c g t t g t t t t a a t a g c t g c t c g c g

2760 LINPDGSLLFRVTINGVTGW c t g a t c a a c c c c g a c g g c t c c c t g c t g t t c c g a g t a a c c a t c a a c g g a g t g a c c g g c t g g g a c t a g t t g g g g c t g c c g a g g g a c g a c a a g g c t c a t t g g t a g t t g c c t c a c t g g c c g a c c

EcoRIAcc65IKpnI 2820 RLCERILAGGSGGTKDEL* c g g c t g t g c g a a c g c a t t c t g g c g g g t g g a t c a g g c g g t a c c a a g g a c g a g c t g t a a g a a g c c g a c a c g c t t g c g t a a g a c c g c c c a c c t a g t c c g c c a t g g t t c c t g c t c g a c a t t c t t

XbaIPspOMIApaIPaeR7IPspXIXhoIEcoRV 2880 SP6 promoter G A SP6 t t c t g c a g a t a t c c a t c a c a c t g g c g g c c g c t c g a g c a t g c a t c t a g a g g g c c c t a t t c t a a g a c g t c t a t a g g t a g t g t g a c c g c c g g c g a g c t c g t a c g t a g a t c t c c c g g g a t a a g a

2940 SP6 promoter bGH poly(A) signal T A T C A C A G T G G A T T T A SP6 G G A G C T G A C A C G G A A G A T BGH-rev a t a g t g t c a c c t a a a t g c t a g a g c t c g c t g a t c a g c c t c g a c t g t g c c t t c t a g t t g c c a t a t c a c a g t g g a t t t a c g a t c t c g a g c g a c t a g t c g g a g c t g a c a c g g a a g a t c a a c g g t

3000 bGH poly(A) signal g c c a t c t g t t g t t t g c c c c t c c c c c g t g c c t t c c t t g a c c c t g g a a g g t g c c a c t c c c a c c g g t a g a c a a c a a a c g g g g a g g g g g c a c g g a a g g a a c t g g g a c c t t c c a c g g t g a g g g t g

3060 bGH poly(A) signal t g t c c t t t c c t a a t a a a a t g a g g a a a t t g c a t c g c a t t g t c t g a g t a g g t g t c a t t c t a t a c a g g a a a g g a t t a t t t t a c t c c t t t a a c g t a g c g t a a c a g a c t c a t c c a c a g t a a g a t a

3120 bGH poly(A) signal t c t g g g g g g t g g g g t g g g g c a g g a c a g c a a g g g g g a g g a t t g g g a a g a c a a t a g c a g g c a a g a c c c c c c a c c c c a c c c c g t c c t g t c g t t c c c c c t c c t a a c c c t t c t g t t a t c g t c c g t

3180 bGH poly(A) signal t g c t g g g g a t g c g g t g g g c t c t a t g g c t t c t g a g g c g g a a a g a a c c a g c t g g g g c t c t a g a c g a c c c c t a c g c c a c c c g a g a t a c c g a a g a c t c c g c c t t t c t t g g t c g a c c c c g a g a t c

3240 f1 ori g g g g t a t c c c c a c g c g c c c t g t a g c g g c g c a t t a a g c g c g g c g g g t g t g g t g g t t a c g c g c c c c a t a g g g g t g c g c g g g a c a t c g c c g c g t a a t t c g c g c c g c c c a c a c c a c c a a t g c g c

3300 f1 ori G A G G A A A G C G A A A G A A G G G A F1ori-R c a g c g t g a c c g c t a c a c t t g c c a g c g c c c t a g c g c c c g c t c c t t t c g c t t t c t t c c c t t c g t c g c a c t g g c g a t g t g a a c g g t c g c g g g a t c g c g g g c g a g g a a a g c g a a a g a a g g g a a g

3360 f1 ori c t t t c t c g c c a c g t t c g c c g g c t t t c c c c g t c a a g c t c t a a a t c g g g g g c t c c c t t t a g g g a a a g a g c g g t g c a a g c g g c c g a a a g g g g c a g t t c g a g a t t t a g c c c c c g a g g g a a a t c c

3420 f1 ori g t t c c g a t t t a g t g c t t t a c g g c a c c t c g a c c c c a a a a a a c t t g a t t a g g g t g a t g g t t c c a a g g c t a a a t c a c g a a a t g c c g t g g a g c t g g g g t t t t t t g a a c t a a t c c c a c t a c c a a g

DraIII 3480 f1 ori a c g t a g t g g g c c a t c g c c c t g a t a g a c g g t t t t t c g c c c t t t g a c g t t g g a g t c c a c g t t t g c a t c a c c c g g t a g c g g g a c t a t c t g c c a a a a a g c g g g a a a c t g c a a c c t c a g g t g c a a

3540 f1 ori G T G G A C T C T T G T T C C A A A C T G G F1ori-F c t t t a a t a g t g g a c t c t t g t t c c a a a c t g g a a c a a c a c t c a a c c c t a t c t c g g t c t a t t c g a a a t t a t c a c c t g a g a a c a a g g t t t g a c c t t g t t g t g a g t t g g g a t a g a g c c a g a t a a g

3600 f1 ori t t t t g a t t t a t a a g g g a t t t t g c c g a t t t c g g c c t a t t g g t t a a a a a a t g a g c t g a t t t a a a a a c t a a a t a t t c c c t a a a a c g g c t a a a g c c g g a t a a c c a a t t t t t t a c t c g a c t a a a t

3660 f1 ori SV40 promoter C C T T A C A C A C A G T C A A T C C C A pBABE 3' a c a a a a a t t t a a c g c g a a t t a a t t c t g t g g a a t g t g t g t c a g t t a g g g t g t g g a a a g t c c t g t t t t t a a a t t g c g c t t a a t t a a g a c a c c t t a c a c a c a g t c a a t c c c a c a c c t t t c a g g

3720 SV40 promoter c c a g g c t c c c c a g c a g g c a g a a g t a t g c a a a g c a t g c a t c t c a a t t a g t c a g c a a c c a g g g g t c c g a g g g g t c g t c c g t c t t c a t a c g t t t c g t a c g t a g a g t t a a t c a g t c g t t g g t c c

3780 SV40 promoter t g t g g a a a g t c c c c a g g c t c c c c a g c a g g c a g a a g t a t g c a a a g c a t g c a t c t c a a t t a g a c a c c t t t c a g g g g t c c g a g g g g t c g t c c g t c t t c a t a c g t t t c g t a c g t a g a g t t a a t c

3840 SV40 promoter SV40 ori t c a g c a a c c a t a g t c c c g c c c c t a a c t c c g c c c a t c c c g c c c c t a a c t c c g c c c a g t t c c a g t c g t t g g t a t c a g g g c g g g g a t t g a g g c g g g t a g g g c g g g g a t t g a g g c g g g t c a a g g

3900 SV40 promoter SV40 ori T A T T T A T G C A G A G G C C G A G G SV40pro-F g c c c a t t c t c c g c c c c a t g g c t g a c t a a t t t t t t t t a t t t a t g c a g a g g c c g a g g c c g c c c g g g t a a g a g g c g g g g t a c c g a c t g a t t a a a a a a a a t a a a t a c g t c t c c g g c t c c g g c g g

AvrII 3960 SV40 promoter SV40 ori t c t g c c t c t g a g c t a t t c c a g a a g t a g t g a g g a g g c t t t t t t g g a g g c c t a g g c t t t t g c a g a c g g a g a c t c g a t a a g g t c t t c a t c a c t c c t c c g a a a a a a c c t c c g g a t c c g a a a a c g

TspMIXmaISmaI 4020 SV40 promoter a a a a a g c t c c c g g g a g c t t g t a t a t c c a t t t t c g g a t c t g a t c a a g a g a c a g g a t g a g g a t t t t t c g a g g g c c c t c g a a c a t a t a g g t a a a a g c c t a g a c t a g t t c t c t g t c c t a c t c c t

4080 1 5 10 15 MIEQDGLHAGSPAAWVE NeoR/KanR MIEQDGLHAGSPAAWVE t c g t t t c g c a t g a t t g a a c a a g a t g g a t t g c a c g c a g g t t c t c c g g c c g c t t g g g t g g a g a g c a a a g c g t a c t a a c t t g t t c t a c c t a a c g t g c g t c c a a g a g g c c g g c g a a c c c a c c t c

4140 20 25 30 35 RLFGYDWAQQTIGCSDAAVF NeoR/KanR G A T A A G C C G A T A C T G A C C C G Neo-R RLFGYDWAQQTIGCSDAAVF a g g c t a t t c g g c t a t g a c t g g g c a c a a c a g a c a a t c g g c t g c t c t g a t g c c g c c g t g t t c t c c g a t a a g c c g a t a c t g a c c c g t g t t g t c t g t t a g c c g a c g a g a c t a c g g c g g c a c a a g

4200 40 45 50 55 RLSAQGRPVLFVKTDLSGAL NeoR/KanR RLSAQGRPVLFVKTDLSGAL c g g c t g t c a g c g c a g g g g c g c c c g g t t c t t t t t g t c a a g a c c g a c c t g t c c g g t g c c c t g g c c g a c a g t c g c g t c c c c g c g g g c c a a g a a a a a c a g t t c t g g c t g g a c a g g c c a c g g g a c

4260 60 65 70 75 NELQDEAARLSWLATTGVPC NeoR/KanR NELQDEAARLSWLATTGVPC a a t g a a c t g c a g g a c g a g g c a g c g c g g c t a t c g t g g c t g g c c a c g a c g g g c g t t c c t t g c t t a c t t g a c g t c c t g c t c c g t c g c g c c g a t a g c a c c g a c c g g t g c t g c c c g c a a g g a a c g

4320 80 85 90 95 AAVLDVVTEAGRDWLLLGEV NeoR/KanR AAVLDVVTEAGRDWLLLGEV g c a g c t g t g c t c g a c g t t g t c a c t g a a g c g g g a a g g g a c t g g c t g c t a t t g g g c g a a g t g c g t c g a c a c g a g c t g c a a c a g t g a c t t c g c c c t t c c c t g a c c g a c g a t a a c c c g c t t c a c

4380 100 105 110 115 PGQDLLSSHLAPAEKVSIMA NeoR/KanR PGQDLLSSHLAPAEKVSIMA *RVKSRGLFYGDHS c c g g g g c a g g a t c t c c t g t c a t c t c a c c t t g c t c c t g c c g a g a a a g t a t c c a t c a t g g c t g g c c c c g t c c t a g a g g a c a g t a g a g t g g a a c g a g g a c g g c t c t t t c a t a g g t a g t a c c g a

4440 120 125 130 135 DAMRRLHTLDPATCPFDHQA NeoR/KanR DAMRRLHTLDPATCPFDHQA ICHPPQMRKIRSGAWEVVLR g a t g c a a t g c g g c g g c t g c a t a c g c t t g a t c c g g c t a c c t g c c c a t t c g a c c a c c a a g c g c t a c g t t a c g c c g c c g a c g t a t g c g a a c t a g g c c g a t g g a c g g g t a a g c t g g t g g t t c g c

4500 140 145 150 155 KHRIERARTRMEAGLVDQDD NeoR/KanR KHRIERARTRMEAGLVDQDD FMADLSCTSPHFGTKDILII a a a c a t c g c a t c g a g c g a g c a c g t a c t c g g a t g g a a g c c g g t c t t g t c g a t c a g g a t g a t t t t g t a g c g t a g c t c g c t c g t g c a t g a g c c t a c c t t c g g c c a g a a c a g c t a g t c c t a c t a

BssHII 4560 160 165 170 175 LDEEHQGLAPAELFARLKAR NeoR/KanR LDEEHQGLAPAELFARLKAR QVFLMLPERWGFQEGPELRA c t g g a c g a a g a g c a t c a g g g g c t c g c g c c a g c c g a a c t g t t c g c c a g g c t c a a g g c g c g c g a c c t g c t t c t c g t a g t c c c c g a g c g c g g t c g g c t t g a c a a g c g g t c c g a g t t c c g c g c g

4620 180 185 190 195 MPDGEDLVVTHGDACLPNIM NeoR/KanR MPDGEDLVVTHGDACLPNIM HGVALIEDHGMAIGAQRIDH a t g c c c g a c g g c g a g g a t c t c g t c g t g a c c c a t g g c g a t g c c t g c t t g c c g a a t a t c a t g t a c g g g c t g c c g c t c c t a g a g c a g c a c t g g g t a c c g c t a c g g a c g a a c g g c t t a t a g t a c

RsrII 4680 200 205 210 215 VENGRFSGFIDCGRLGVADR NeoR/KanR VENGRFSGFIDCGRLGVADR HFIAAKRSEDVTAPQTHRVA g t g g a a a a t g g c c g c t t t t c t g g a t t c a t c g a c t g t g g c c g g c t g g g t g t g g c g g a c c g c c a c c t t t t a c c g g c g a a a a g a c c t a a g t a g c t g a c a c c g g c c g a c c c a c a c c g c c t g g c g

4740 220 225 230 235 YQDIALATRDIAEELGGEWA NeoR/KanR C G T T G G C T A C C C G T G A T A T T Neo-F YQDIALATRDIAEELGGEWA ILVYRQSGTINSFLKAAFPS t a t c a g g a c a t a g c g t t g g c t a c c c g t g a t a t t g c t g a a g a g c t t g g c g g c g a a t g g g c t a t a g t c c t g t a t c g c a a c c g a t g g g c a c t a t a a c g a c t t c t c g a a c c g c c g c t t a c c c g a

4800 240 245 250 255 DRFLVLYGIAAPDSQRIAFY NeoR/KanR DRFLVLYGIAAPDSQRIAFY VAEEHKVTDGSGIRLADGEI g a c c g c t t c c t c g t g c t t t a c g g t a t c g c c g c t c c c g a t t c g c a g c g c a t c g c c t t c t a t c t g g c g a a g g a g c a c g a a a t g c c a t a g c g g c g a g g g c t a a g c g t c g c g t a g c g g a a g a t a

BstBI 4860 260 265 RLLDEFF* NeoR/KanR RLLDEFF* AKKVLEESRSEPTRFSRGLS c g c c t t c t t g a c g a g t t c t t c t g a g c g g g a c t c t g g g g t t c g a a a t g a c c g a c c a a g c g a g c g g a a g a a c t g c t c a a g a a g a c t c g c c c t g a g a c c c c a a g c t t t a c t g g c t g g t t c g c t

4920 AWGAM c g c c c a a c c t g c c a t c a c g a g a t t t c g a t t c c a c c g c c g c c t t c t a t g a a a g g t t g g g c t g c g g g t t g g a c g g t a g t g c t c t a a a g c t a a g g t g g c g g c g g a a g a t a c t t t c c a a c c c g a

PfoI 4980 t c g g a a t c g t t t t c c g g g a c g c c g g c t g g a t g a t c c t c c a g c g c g g g g a t c t c a t g c t g g a g c c t t a g c a a a a g g c c c t g c g g c c g a c c t a c t a g g a g g t c g c g c c c c t a g a g t a c g a c c

5040 SV40 poly(A) signal C G T T A T SV40pA-R a g t t c t t c g c c c a c c c c a a c t t g t t t a t t g c a g c t t a t a a t g g t t a c a a a t a a a g c a a t a t c a a g a a g c g g g t g g g g t t g a a c a a a t a a c g t c g a a t a t t a c c a a t g t t t a t t t c g t t a t

5100 SV40 poly(A) signal G T G G T T T G T C C A EBV-rev C G T A G T G T T T A A A G SV40pA-R g c a t c a c a a a t t t c a c a a a t a a a g c a t t t t t t t c a c t g c a t t c t a g t t g t g g t t t g t c c a c g t a g t g t t t a a a g t g t t t a t t t c g t a a a a a a a g t g a c g t a a g a t c a a c a c c a a a c a g g t

BstZ17I 5160 SV40 poly(A) signal A A C T C A T C EBV-rev a a c t c a t c a a t g t a t c t t a t c a t g t c t g t a t a c c g t c g a c c t c t a g c t a g a g c t t g g c g t t t g a g t a g t t a c a t a g a a t a g t a c a g a c a t a t g g c a g c t g g a g a t c g a t c t c g a a c c g c a

5220 -10 M13 rev lac operator lac promoter C A G T A T C G A C A A A G G A C M13 Reverse G G A C A C A C T T T A A C A A T A G G C G A M13/pUC Reverse a a t c a t g g t c a t a g c t g t t t c c t g t g t g a a a t t g t t a t c c g c t c a c a a t t c c a c a c a a c a t t a g t a c c a g t a t c g a c a a a g g a c a c a c t t t a a c a a t a g g c g a g t g t t a a g g t g t g t t g t

5280 -35 lac promoter CAP binding site t a c g a g c c g g a a g c a t a a a g t g t a a a g c c t g g g g t g c c t a a t g a g t g a g c t a a c t c a c a t a t g c t c g g c c t t c g t a t t t c a c a t t t c g g a c c c c a c g g a t t a c t c a c t c g a t t g a g t g t a

5340 CAP binding site t a a t t g c g t t g c g c t c a c t g c c c g c t t t c c a g t c g g g a a a c c t g t c g t g c c a g c t g c a t t a t t a a c g c a a c g c g a g t g a c g g g c g a a a g g t c a g c c c t t t g g a c a g c a c g g t c g a c g t a a

5400 G A L4440 a a t g a a t c g g c c a a c g c g c g g g g a g a g g c g g t t t g c g t a t t g g g c g c t c t t c c g c t t c c t t t a c t t a g c c g g t t g c g c g c c c c t c t c c g c c a a a c g c a t a a c c c g c g a g a a g g c g a a g g a

5460 G C G A G T G A C T G A G C G A L4440 c g c t c a c t g a c t c g c t g c g c t c g g t c g t t c g g c t g c g g c g a g c g g t a t c a g c t c a c t c a a g c g a g t g a c t g a g c g a c g c g a g c c a g c a a g c c g a c g c c g c t c g c c a t a g t c g a g t g a g t t

PciI 5520 a g g c g g t a a t a c g g t t a t c c a c a g a a t c a g g g g a t a a c g c a g g a a a g a a c a t g t g a g c a a t c c g c c a t t a t g c c a a t a g g t g t c t t a g t c c c c t a t t g c g t c c t t t c t t g t a c a c t c g t t

5580 ori a a g g c c a g c a a a a g g c c a g g a a c c g t a a a a a g g c c g c g t t g c t g g c g t t t t t c c a t a g g c t t c c g g t c g t t t t c c g g t c c t t g g c a t t t t t c c g g c g c a a c g a c c g c a a a a a g g t a t c c g

5640 ori t c c g c c c c c c t g a c g a g c a t c a c a a a a a t c g a c g c t c a a g t c a g a g g t g g c g a a a c c c g a a g g c g g g g g g a c t g c t c g t a g t g t t t t t a g c t g c g a g t t c a g t c t c c a c c g c t t t g g g c t

5700 ori T T T C T A T G G T C C G C A A A G G G pBR322ori-F c a g g a c t a t a a a g a t a c c a g g c g t t t c c c c c t g g a a g c t c c c t c g t g c g c t c t c c t g t t c g t c c t g a t a t t t c t a t g g t c c g c a a a g g g g g a c c t t c g a g g g a g c a c g c g a g a g g a c a a g

5760 ori c g a c c c t g c c g c t t a c c g g a t a c c t g t c c g c c t t t c t c c c t t c g g g a a g c g t g g c g c t t t g c t g g g a c g g c g a a t g g c c t a t g g a c a g g c g g a a a g a g g g a a g c c c t t c g c a c c g c g a a a

5820 ori c t c a t a g c t c a c g c t g t a g g t a t c t c a g t t c g g t g t a g g t c g t t c g c t c c a a g c t g g g c t g a g t a t c g a g t g c g a c a t c c a t a g a g t c a a g c c a c a t c c a g c a a g c g a g g t t c g a c c c g a

5880 ori g t g t g c a c g a a c c c c c c g t t c a g c c c g a c c g c t g c g c c t t a t c c g g t a a c t a t c g t c t t g c a c a c g t g c t t g g g g g g c a a g t c g g g c t g g c g a c g c g g a a t a g g c c a t t g a t a g c a g a a c

5940 ori a g t c c a a c c c g g t a a g a c a c g a c t t a t c g c c a c t g g c a g c a g c c a c t g g t a a c a g g a t t a t c a g g t t g g g c c a t t c t g t g c t g a a t a g c g g t g a c c g t c g t c g g t g a c c a t t g t c c t a a t

6000 ori g c a g a g c g a g g t a t g t a g g c g g t g c t a c a g a g t t c t t g a a g t g g t g g c c t a a c t a c g g c t c g t c t c g c t c c a t a c a t c c g c c a c g a t g t c t c a a g a a c t t c a c c a c c g g a t t g a t g c c g a

6060 ori a c a c t a g a a g a a c a g t a t t t g g t a t c t g c g c t c t g c t g a a g c c a g t t a c c t t c g g a a a a a t g t g a t c t t c t t g t c a t a a a c c a t a g a c g c g a g a c g a c t t c g g t c a a t g g a a g c c t t t t t

6120 ori g a g t t g g t a g c t c t t g a t c c g g c a a a c a a a c c a c c g c t g g t a g c g g t g g t t t t t t t g t t t c t c a a c c a t c g a g a a c t a g g c c g t t t g t t t g g t g g c g a c c a t c g c c a c c a a a a a a a c a a a

6180 ori g c a a g c a g c a g a t t a c g c g c a g a a a a a a a g g a t c t c a a g a a g a t c c t t t g a t c t t t t c t a c g t t c g t c g t c t a a t g c g c g t c t t t t t t t c c t a g a g t t c t t c t a g g a a a c t a g a a a a g a t

6240 c g g g g t c t g a c g c t c a g t g g a a c g a a a a c t c a c g t t a a g g g a t t t t g g t c a t g a g a t t a t g c c c c a g a c t g c g a g t c a c c t t g c t t t t g a g t g c a a t t c c c t a a a a c c a g t a c t c t a a t a

6300 c a a a a a g g a t c t t c a c c t a g a t c c t t t t a a a t t a a a a a t g a a g t t t t a a a t c a a t c t a a a g t t t t t c c t a g a a g t g g a t c t a g g a a a a t t t a a t t t t t a c t t c a a a a t t t a g t t a g a t t t

6360 285 280 *WHKILSAGIE AmpR *WHKILSAGIE g t a t a t a t g a g t a a a c t t g g t c t g a c a g t t a c c a a t g c t t a a t c a g t g a g g c a c c t a t c t c a t a t a t a c t c a t t t g a a c c a g a c t g t c a a t g g t t a c g a a t t a g t c a c t c c g t g g a t a g a

6420 275 270 265 260 AIQRNREDMTAQSGTTYIVV AmpR AIQRNREDMTAQSGTTYIVV c a g c g a t c t g t c t a t t t c g t t c a t c c a t a g t t g c c t g a c t c c c c g t c g t g t a g a t a a c t a g t c g c t a g a c a g a t a a a g c a a g t a g g t a t c a a c g g a c t g a g g g g c a g c a c a t c t a t t g a t

6480 255 250 245 240 IRSPKGDPGLAAIIGRSGRE AmpR IRSPKGDPGLAAIIGRSGRE c g a t a c g g g a g g g c t t a c c a t c t g g c c c c a g t g c t g c a a t g a t a c c g c g a g a c c c a c g c t g c t a t g c c c t c c c g a a t g g t a g a c c g g g g t c a c g a c g t t a c t a t g g c g c t c t g g g t g c g a

6540 235 230 225 220 GAGSKDAIFWGAPLASRLLP AmpR GAGSKDAIFWGAPLASRLLP c a c c g g c t c c a g a t t t a t c a g c a a t a a a c c a g c c a g c c g g a a g g g c c g a g c g c a g a a g t g g t g g c c g a g g t c t a a a t a g t c g t t a t t t g g t c g g t c g g c c t t c c c g g c t c g c g t c t t c a c

6600 215 210 205 200 GAVKDAEMWDILQQRSALTL AmpR GAVKDAEMWDILQQRSALTL g t c c t g c a a c t t t a t c c g c c t c c a t c c a g t c t a t t a a t t g t t g c c g g g a a g c t a g a g t a a c a g g a c g t t g a a a t a g g c g g a g g t a g g t c a g a t a a t t a a c a a c g g c c c t t c g a t c t c a t t

6660 195 190 185 180 LEGTLLKRLTTAMAVPMTTD AmpR LEGTLLKRLTTAMAVPMTTD g t a g t t c g c c a g t t a a t a g t t t g c g c a a c g t t g t t g c c a t t g c t a c a g g c a t c g t g g t g t c a t c a a g c g g t c a a t t a t c a a a c g c g t t g c a a c a a c g g t a a c g a t g t c c g t a g c a c c a c a

6720 175 170 165 160 REDNPIAENLEPEWRDLRTV AmpR REDNPIAENLEPEWRDLRTV c a c g c t c g t c g t t t g g t a t g g c t t c a t t c a g c t c c g g t t c c c a a c g a t c a a g g c g a g t t a g t g c g a g c a g c a a a c c a t a c c g a a g t a a g t c g a g g c c a a g g g t t g c t a g t t c c g c t c a a t

PvuI 6780 155 150 145 140 HDGMNHLFATLEKPGGITTL AmpR HDGMNHLFATLEKPGGITTL c a t g a t c c c c c a t g t t g t g c a a a a a a g c g g t t a g c t c c t t c g g t c c t c c g a t c g t t g t c a g t a c t a g g g g g t a c a a c a c g t t t t t t c g c c a a t c g a g g a a g c c a g g a g g c t a g c a a c a g t

6840 135 130 125 120 LLNAATNDSMTIAASCLERV AmpR LLNAATNDSMTIAASCLERV g a a g t a a g t t g g c c g c a g t g t t a t c a c t c a t g g t t a t g g c a g c a c t g c a t a a t t c t c t t a c t t c a t t c a a c c g g c g t c a c a a t a g t g a g t a c c a a t a c c g t c g t g a c g t a t t a a g a g a a t

ScaI 6900 115 110 105 100 TMGDTLHKETVPSYEVLDNQ AmpR TMGDTLHKETVPSYEVLDNQ c t g t c a t g c c a t c c g t a a g a t g c t t t t c t g t g a c t g g t g a g t a c t c a a c c a a g t c a t t c t g a c a g t a c g g t a g g c a t t c t a c g a a a a g a c a c t g a c c a c t c a t g a g t t g g t t c a g t a a g a

6960 95 90 85 80 SYHIRRGLQEQGADIRSLVA AmpR A T A A T A C C G Amp-R SYHIRRGLQEQGADIRSLVA g a g a a t a g t g t a t g c g g c g a c c g a g t t g c t c t t g c c c g g c g t c a a t a c g g g a t a a t a c c g c t c t t a t c a c a t a c g c c g c t g g c t c a a c g a g a a c g g g c c g c a g t t a t g c c c t a t t a t g g c

7020 75 70 65 60 GCLLVKFTSMMPFREEPRFS AmpR C G C C A C A T A G C Amp-R GCLLVKFTSMMPFREEPRFS c g c c a c a t a g c a g a a c t t t a a a a g t g c t c a t c a t t g g a a a a c g t t c t t c g g g g c g a a a a c g c g g t g t a t c g t c t t g a a a t t t t c a c g a g t a g t a a c c t t t t g c a a g a a g c c c c g c t t t t g

7080 55 50 45 40 ELIKGSNLDLEIYGVRAGLQ AmpR ELIKGSNLDLEIYGVRAGLQ t c t c a a g g a t c t t a c c g c t g t t g a g a t c c a g t t c g a t g t a a c c c a c t c g t g c a c c c a a c t a g a g t t c c t a g a a t g g c g a c a a c t c t a g g t c a a g c t a c a t t g g g t g a g c a c g t g g g t t g a

7140 35 30 25 DEADKVKVLTEPH 20 signal sequence AFVPLCF AmpR DEADKVKVLTEPHAFVPLCF g a t c t t c a g c a t c t t t t a c t t t c a c c a g c g t t t c t g g g t g a g c a a a a a c a g g a a g g c a a a c t a g a a g t c g t a g a a a a t g a a a g t g g t c g c a a a g a c c c a c t c g t t t t t g t c c t t c c g t t t

7200 15 10 5 1 signal sequence AAFFPILAVRFHQISM AmpR AmpR promoter AAFFPILAVRFHQISM a t g c c g c a a a a a a g g g a a t a a g g g c g a c a c g g a a a t g t t g a a t a c t c a t a c t c t t c c t t t t a c g g c g t t t t t t c c c t t a t t c c c g c t g t g c c t t t a c a a c t t a t g a g t a t g a g a a g g a a a

SspI 7260 AmpR promoter t t c a a t a t t a t t g a a g c a t t t a t c a g g g t t a t t g t c t c a t g a g c g g a t a c a t a t t t g a a t a a g t t a t a a t a a c t t c g t a a a t a g t c c c a a t a a c a g a g t a c t c g c c t a t g t a t a a a c t t a

7320 AmpR promoter g t a t t t a g a a a a a t a a a c a a a t a g g g g t t c c g c g c a c a t t t c c c c g a a a a g t g c c a c c t g c a t a a a t c t t t t t a t t t g t t t a t c c c c a a g g c g c g t g t a a a g g g g c t t t t c a c g g t g g a c

BglIISgrDI 7380 A T G T T A G A C G A G pRS-marker a c g t c g a c g g a t c g g g a g a t c t c c c g a t c c c c t a t g g t g c a c t c t c a g t a c a a t c t g c t c t g c a g c t g c c t a g c c c t c t a g a g g g c t a g g g g a t a c c a c g t g a g a g t c a t g t t a g a c g a g

3ʹ 5ʹ 7406 A C T A C G G C pRS-marker t g a t g c c g c a t a g t t a a g c c a g t a t c a c t a c g g c g t a t c a a t t c g g t c a t a g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.