Addgene pLV-PLXNB2-dVTDL Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: pLV-PLXNB2-dVTDL Sequencing Result


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Neo-R (13,626 .. 13,645) RsrII (13,051) Neo-F (13,016 .. 13,035) KpnI (12,884) Acc65I (12,880) BmtI (12,806) NheI (12,802) SV40pA-R (12,453 .. 12,472) EBV-rev (12,399 .. 12,418) AvrII (12,209) T7 promoter T7 (12,174 .. 12,193) M13 fwd M13 Forward (12,147 .. 12,164) M13/pUC Forward (12,133 .. 12,155) F1ori-R (11,900 .. 11,919) F1ori-F (11,688 .. 11,709) Amp-R (11,183 .. 11,202) pBR322ori-F (9881 .. 9900) PciI (9740) L4440 (9630 .. 9647) CAP binding site lac promoter M13/pUC Reverse (9412 .. 9434) lac operator M13 Reverse (9399 .. 9415) M13 rev T3 (9360 .. 9380) T3 promoter MfeI (8369) BbvCI (8135) HpaI (7534) BspDI * - ClaI * (7409) PaeR7I - XhoI (7390) AscI (7018) hPGK-F (6921 .. 6939) mPGK-F (109 .. 128) MSCV-rev (502 .. 525) WPRE-R (1118 .. 1138) BsaBI (2545) BclI * (2867) AfeI (3355) FseI (4240) PshAI (4767) attB1 SalI (6836) pLV-PLXNB2-dVTDL 13,661 bp

Sequence View

5ʹ 3ʹ 60 15 10 5 1 APSGAHLGDQEIASGM NeoR/KanR PSGAHLGDQEIASGM g c c g g a g a a c c t g c g t g c a a t c c a t c t t g t t c a a t g g c c g a t c c c a t a t t g g c t g c a g g t c g g c c t c t t g g a c g c a c g t t a g g t a g a a c a a g t t a c c g g c t a g g g t a t a a c c g a c g t c c a

120 PGK promoter G A A A A C T T C G C A mPGK-F c g a a a g g c c c g g a g a t g a g g a a g a g g a g a a c a g c g c g g c a g a c g t g c g c t t t t g a a g c g t g c t t t c c g g g c c t c t a c t c c t t c t c c t c t t g t c g c g c c g t c t g c a c g c g a a a a c t t c g c a

180 PGK promoter C G T C T T A C mPGK-F g c a g a a t g c c g g g c c t c c g g a g g a c c t t c g g g c g c c c g c c c c g c c c c t g a g c c c g c c c c t c g t c t t a c g g c c c g g a g g c c t c c t g g a a g c c c g c g g g c g g g g c g g g g a c t c g g g c g g g g a

240 PGK promoter g a g c c c g c c c c c g g a c c c a c c c c t t c c c a g c c t c t g a g c c c a g a a a g c g a a g g a g c a a a g c t c g g g c g g g g g c c t g g g t g g g g a a g g g t c g g a g a c t c g g g t c t t t c g c t t c c t c g t t t c

300 PGK promoter c t g c t a t t g g c c g c t g c c c c a a a g g c c t a c c c g c t t c c a t t g c t c a g c g g t g c t g t c c a t g a c g a t a a c c g g c g a c g g g g t t t c c g g a t g g g c g a a g g t a a c g a g t c g c c a c g a c a g g t a

360 PGK promoter c t g c a c g a g a c t a g t g a g a c g t g c t a c t t c c a t t t g t c a c g t c c t g c a c g a c g c g a g c t g g a c g t g c t c t g a t c a c t c t g c a c g a t g a a g g t a a a c a g t g c a g g a c g t g c t g c g c t c g a c

420 PGK promoter c g g g g c g g g g g g g a a c t t c c t g a c t a g g g g a g g a g t a g a a g g t g g c g c g a a g g g g c c a c c g c c c c g c c c c c c c t t g a a g g a c t g a t c c c c t c c t c a t c t t c c a c c g c g c t t c c c c g g t g g

480 PGK promoter a a a g a a c g g a g c c g g t t g g c g c c t a c c g g t g g a t g t g g a a t g t g t g c g a g g c c a g a g g c c t t t c t t g c c t c g g c c a a c c g c g g a t g g c c a c c t a c a c c t t a c a c a c g c t c c g g t c t c c g g

540 PGK promoter C A G C G G G G C T G C T A A A G C G C A T G C MSCV-rev a c t t g t g t a g c g c c a a g t g c c c a g c g g g g c t g c t a a a g c g c a t g c t c c a g a c t g c c t t g g t g a a c a c a t c g c g g t t c a c g g g t c g c c c c g a c g a t t t c g c g t a c g a g g t c t g a c g g a a c c

600 PGK promoter *IVLGSIQLIG g a a a a g c g c c t c c c c t a c c c g g t a g a a t t a g a t c a c t a g t c c g g a t a t c t g c a g a a t t c c c t t t t c g c g g a g g g g a t g g g c c a t c t t a a t c t a g t g a t c a g g c c t a t a g a c g t c t t a a g g

660 WPRE PPSAAWLSIRSTQPRLRLRP a g g c g g g g a g g c g g c c c a a a g g g a g a t c c g a c t c g t c t g a g g g c g a a g g c g a a g a c g c g g t c c g c c c c t c c g c c g g g t t t c c c t c t a g g c t g a g c a g a c t c c c g c t t c c g c t t c t g c g c c

720 WPRE 1 IEGR Factor Xa site LPRLAPLLGRSPLDAPNLAS a a g a g g c c g c a g a g c c g g c a g c a g g c c g c g g g a a g g a a g g t c c g c t g g a t t g a g g g c c g a t t c t c c g g c g t c t c g g c c g t c g t c c g g c g c c c t t c c t t c c a g g c g a c c t a a c t c c c g g c t

780 WPRE (in frame with Factor Xa site) RDVAEGRPAQNPGGNTGEQP PVYCFSTGRLIWTAVCALLW a g g g a c g t a g c a g a a g g a c g t c c c g c g c a g a a t c c a g g t g g c a a c a c a g g c g a g c a g c c a t c c c t g c a t c g t c t t c c t g c a g g g c g c g t c t t a g g t c c a c c g t t g t g t c c g c t c g t c g g t

840 WPRE (in frame with Factor Xa site) WKGRQLPRQHHGIVSAQQPS PFSTLKGSLVVSNDTGLLRA t g g a a a g g a c g t c a g c t t c c c c g a c a a c a c c a c g g a a t t g t c a g t g c c c a a c a g c c g a g c a c c t t t c c t g c a g t c g a a g g g g c t g t t g t g g t g c c t t a a c a g t c a c g g g t t g t c g g c t c g

900 WPRE (in frame with Factor Xa site) PCPAAGKAGGDEFRRGNREG GTWCRALCAAILEATAIPLP c c c t g t c c a g c a g c g g g c a a g g c a g g c g g c g a t g a g t t c c g c c g t g g c a a t a g g g a g g g g g g g a c a g g t c g t c g c c c g t t c c g t c c g c c g c t a c t c a a g g c g g c a c c g t t a t c c c t c c c c

960 WPRE (in frame with Factor Xa site) ESESPGKELTGGGNAPTSGG FAFTGSLLQCTTAIGWGTPT g a a a g c g a a a g t c c c g g a a a g g a g c t g a c a g g t g g t g g c a a t g c c c c a a c c a g t g g g g g t c t t t c g c t t t c a g g g c c t t t c c t c g a c t g t c c a c c a c c g t t a c g g g g t t g g t c a c c c c c a

1020 WPRE (in frame with Factor Xa site) CVSKHSAHHATLPDNGPQLL ADAFVTCVVGRQRVVPWLEE t g c g t c a g c a a a c a c a g t g c a c a c c a c g c c a c g t t g c c t g a c a a c g g g c c a c a a c t c c t c a c g c a g t c g t t t g t g t c a c g t g t g g t g c g g t g c a a c g g a c t g t t g c c c g g t g t t g a g g a g

1080 WPRE (in frame with Factor Xa site) IKRQQPGFIQGGENESHTGS YLSLLWSKYLSSFIFAMRSA a t a a a g a g a c a g c a a c c a g g a t t t a t a c a a g g a g g a g a a a a t g a a a g c c a t a c g g g a a g c t a t t t c t c t g t c g t t g g t c c t a a a t a t g t t c c t c c t c t t t t a c t t t c g g t a t g c c c t t c g

1140 WPRE (in frame with Factor Xa site) NSMIQRH* C A T A G C G T A A A A G G A G C A A C A WPRE-R IAHYLPM a a t a g c a t g a t a c a a a g g c a t t a a a g c a g c g t a t c c a c a t a g c g t a a a a g g a g c a a c a t a t t a t c g t a c t a t g t t t c c g t a a t t t c g t c g c a t a g g t g t a t c g c a t t t t c c t c g t t g t a t

1200 WPRE g t t a a g a a t a c c a g t c a a t c t t t c a c a a a t t t t g t a a t c c a g a g g t t g a t t g t c g a g c g g c a a t t c t t a t g g t c a g t t a g a a a g t g t t t a a a a c a t t a g g t c t c c a a c t a a c a g c t c g c c

1260 attB2 * c c g c c a c t g t g c t g g a t a t c a a c c a c t t t g t a c a a g a a a g c t g g g c c c a t g a g g c c c t a c g g c g g t g a c a c g a c c t a t a g t t g g t g a a a c a t g t t c t t t c g a c c c g g g t a c t c c g g g a t g

1320 KNELAAAIQQLRFALQMKQA t t g t t c t c c a g t g c a g c g g c a a t c t g c t g c a g g c g g a a g g c c a g c t g c a t c t t c t g g g c g a a c a a g a g g t c a c g t c g c c g t t a g a c g a c g t c c g c c t t c c g g t c g a c g t a g a a g a c c c g c

1380 APDEELANIIEDYYKQTYQY g c a g g a t c c t c c t c c a a g g c a t t g a t g a t c t c g t c a t a g t a c t t c t g c g t g t a t t g g t a g c g t c c t a g g a g g a g g t t c c g t a a c t a c t a g a g c a g t a t c a t g a a g a c g c a c a t a a c c a t c

1440 LQHLAVLTNLSDTHARSIEA a g c t g g t g g a g t g c c a c g a g g g t g t t c a a g g a g t c c g t g t g c g c c c g g g a a a t c t c t g c c t c g a c c a c c t c a c g g t g c t c c c a c a a g t t c c t c a g g c a c a c g c g g g c c c t t t a g a g a c g g

1500 LHTNMDQDSVQVMQRIGKYY a g g t g t g t g t t c a t g t c c t g g t c g c t g a c c t g c a c c a t c t g c c g g a t c c c c t t g t a g t a a t c c a c a c a c a a g t a c a g g a c c a g c g a c t g g a c g t g g t a g a c g g c c t a g g g g a a c a t c a t t

1560 DEVMKKYTSIEKAYLLKNSP t c c t c c a c c a t c t t c t t g t a g g t g g a g a t c t c c t t g g c g t a c a g c a g c t t g t t g c t g g g a a g g a g g t g g t a g a a g a a c a t c c a c c t c t a g a g g a a c c g c a t g t c g t c g a a c a a c g a c c c t

1620 SDRSLKHETRTCADMFTQAI g a a t c g c g g c t c a g c t t a t g c t c c g t g c g c g t g c a g g c a t c c a t g a a g g t c t g c g c g a t g c t t a g c g c c g a g t c g a a t a c g a g g c a c g c g c a c g t c c g t a g g t a c t t c c a g a c g c g c t a c

1680 VSLSADVVEHVHVDFIFHPN a c t g a c a g c g a g g c g t c c a c c a c c t c g t g g a c a t g c a c g t c a a a g a t g a a g t g g g g g t t c t g a c t g t c g c t c c g c a g g t g g t g g a g c a c c t g t a c g t g c a g t t t c t a c t t c a c c c c c a a g

1740 KLINVWFRLPLSNTKWIHIT t t g a g g a t g t t c a c c c a g a a c c g g a g c g g t a a g c t g t t c g t c t t c c a g a t g t g g a t g g t g a a c t c c t a c a a g t g g g t c t t g g c c t c g c c a t t c g a c a a g c a g a a g g t c t a c a c c t a c c a c

1800 DEDQINHKEAQEDLFDFFYK t c t t c a t c c t g g a t g t t g t g c t t c t c t g c c t g c t c g t c c a g g a a g t c g a a g a a g t a c t t g a g a a g t a g g a c c t a c a a c a c g a a g a g a c g g a c g a g c a g g t c c t t c a g c t t c t t c a t g a a c

1860 VAPPVAHGPALVSQFFNDVF a c t g c a g g t g g c a c c g c g t g c c c a g g c g c c a g c a c g c t c t g g a a g a a g t t g t c c a c a a a c t g a c g t c c a c c g t g g c g c a c g g g t c c g c g g t c g t g c g a g a c c t t c t t c a a c a g g t g t t t g

1920 QQLTGKVSLLRTLYIETIAK t g c t g c a g t g t g c c c t t g a c t g a g a g c a g c c g c g t c a g g t a g a t c t c g g t g a t g g c c t t c a c g a c g t c a c a c g g g a a c t g a c t c t c g t c g g c g c a g t c c a t c t a g a g c c a c t a c c g g a a g

1980 TREKEKVSGRKSKGEDVEDT g t c c g c t c c t t c t c t t t c a c g c t g c c t c t c t t g g a c t t g c c c t c g t c c a c c t c g t c g g t c c a g g c g a g g a a g a g a a a g t g c g a c g g a g a g a a c c t g a a c g g g a g c a g g t g g a g c a g c c a g

2040 PRVLHWVRNEEELLAHREGP g g c c g c a c c a g g t g c c a c a c c c g g t t c t c c t c c t c c a g g a g g g c a t g g c g c t c c c c a g g c c c g g c g t g g t c c a c g g t g t g g g c c a a g a g g a g g a g g t c c t c c c g t a c c g c g a g g g g t c c g

2100 LDQQSDEPQQSVGVKSLILT a g g t c c t g c t g g c t g t c c t c c g g c t g c t g g g a g a c c c c c a c c t t g g a c a g g a t g a g g g t g t c c a g g a c g a c c g a c a g g a g g c c g a c g a c c c t c t g g g g g t g g a a c c t g t c c t a c t c c c a c

2160 AGDRVNYHMLTNVRKWRGER g c t c c a t c c c g g a c a t t g t a g t g c a t a a g g g t g t t g a c g c g c t t c c a c c g g c c c t c c c g c c g a g g t a g g g c c t g t a a c a t c a c g t a t t c c c a c a a c t g c g c g a a g g t g g c c g g g a g g g c g

2220 QSTLDLDSLIQATSGPRWEL t g t g a c g t c a g g t c c a g g t c c g a c a g g a t c t g c g c t g t g g a g c c c g g a c g c c a c t c c a g g a c a c t g c a g t c c a g g t c c a g g c t g t c c t a g a c g c g a c a c c t c g g g c c t g c g g t g a g g t c c

2280 VVSDPRPWCSCPQGRYVQDI a c c a c g c t g t c t g g c c t g g g c c a g c a g g a g c a g g g c t g c c c a c g g t a c a c c t g g t c a a t g t g g t g c g a c a g a c c g g a c c c g g t c g t c c t c g t c c c g a c g g g t g c c a t g t g g a c c a g t t a c

2340 IKEKVQSITDCNLVKVPIAD a t c t t c t c c t t g a c c t g g g a g a t g g t g t c a c a g t t g a g g a c c t t c a c c g g g a t g g c g t c c t a g a a g a g g a a c t g g a c c c t c t a c c a c a g t g t c a a c t c c t g g a a g t g g c c c t a c c g c a g g

2400 VGEDQVIVSVTLPAYEVDDG a c t c c c t c g t c c t g c a c g a t c a c g c t c a c c g t c a g g g g t g c g t a c t c c a c a t c a t c c c c c t g a g g g a g c a g g a c g t g c t a g t g c g a g t g g c a g t c c c c a c g c a t g a g g t g t a g t a g g g g g

2460 LLGTDNLTYKAKKQVADVPG a g c a g c c c c g t g t c g t t g a g a g t g t a c t t g g c c t t c t t c t g t a c c g c a t c c a c c g g g c c c t c g t c g g g g c a c a g c a a c t c t c a c a t g a a c c g g a a g a a g a c a t g g c g t a g g t g g c c c g g g

2520 MFDGLEELVQGLPGTV KEVQHKIAKFLKYLPEGASD t t t t c c a c c t g a t g t t t g a t g g c c t t g a a g a g c t t g t a c a g g g g c t c c c c g g c a c t g t c c a a a a g g t g g a c t a c a a a c t a c c g g a a c t t c t c g a a c a t g t c c c c g a g g g g c c g t g a c a g g

BsaBI 2580 LEVLVQADGHPVGQHPLHHS KLYQYLCISMWNSLMREVVT t t g a g g t a c t g g t a c a g g c a g a t g g a c a t c c a g t t g g a c a g c a t c c t c t c c a c c a c a g t c a a c t c c a t g a c c a t g t c c g t c t a c c t g t a g g t c a a c c t g t c g t a g g a g a g g t g g t g t c a g

2640 LRPAQHQLGVLGHHVLLQEL ESRRLMLKPNKAVVYQELLE t c a g a c c t g c g c a g c a t c a g c t t g g g g t t c t t g g c c a c c a c g t a c t g c t c c a g g a g c t c c a g t c t g g a c g c g t c g t a g t c g a a c c c c a a g a a c c g g t g g t g c a t g a c g a g g t c c t c g a g g

2700 QEERVHDVRVVLQFPVQRHR LFLTHMIDTYYELKGHLAVT a g g a a g a g c g t g t g c a t g a t g t c c g t g t a g t a c t c c a g t t t c c c g t g c a g c g c c a c c g t c t c c t t c t c g c a c a c g t a c t a c a g g c a c a t c a t g a g g t c a a a g g g c a c g t c g c g g t g g c a g

2760 QQGREVDLGAGRELPLVLQG LLSAFYVKARASFERQNELT a g c a g g g a c g c g a a g t a g a c c t t g g c g c g g g c c g a g a a c t c c c g c t g g t t c t c c a g g g t g t c g t c c c t g c g c t t c a t c t g g a a c c g c g c c c g g c t c t t g a g g g c g a c c a a g a g g t c c c a c

2820 VDEIDEERLAVQQVGELVEG HIFNILFSKSNLLNSFQYLA t g g a t g a a a t t g a t g a g g a a a g a c t t g c t g t t c a g c a g g t t g g a g a a c t g g t a g a g g g c c a c c t a c t t t a a c t a c t c c t t t c t g a a c g a c a a g t c g t c c a a c c t c t t g a c c a t c t c c c g g

BclI* 2880 LLHHRPPRLRDVQLAGDHHV QEVVPRRPEPIDLKGTIMVD t g c t c c a c c a c c g g c c g c c g c g g c t c a g g g a t g t c c a g c t t g c c g g t g a t c a t c a c g t c c a c g a g g t g g t g g c c g g c g g c g c c g a g t c c c t a c a g g t c g a a c g g c c a c t a g t a g t g c a g g

2940 LVAVLGGQEEDAVGVGLVVQ KDGDKSPLFFVRDTYTKYDL t t g t c g c c g t c c t t g g a g g g c a g g a a g a a g a c g c g g t c g g t g t a g g t c t t g t a g t c c a g c a a c a g c g g c a g g a a c c t c c c g t c c t t c t t c t g c g c c a g c c a c a t c c a g a a c a t c a g g t c g

3000 HGDAGLVHVVGLVLHLDHQV VPIGAEHVDNTQDEMEIMLD a c g g g g a t g c c g g c c t c g t g c a c g t c g t t g g t c t g g t c c t c c a t c t c g a t c a t c a g g t c t t g c c c c t a c g g c c g g a g c a c g t g c a g c a a c c a g a c c a g g a g g t a g a g c t a g t a g t c c a g a

3060 CEFLLAAVPHALLQALQLGL TFEKKCRDRVSEELGELQSK g t g a a t t c c t t c t t g c a g c g g t c c c g c a c g c t c t c c t c c a g g c c c t c c a g c t g g g a c t t g c a c t t a a g g a a g a a c g t c g c c a g g g c g t g c g a g a g g a g g t c c g g g a g g t c g a c c c t g a a c

3120 DLLILSFGLLALPPVAVDRH IKEYEREAQQSKRWYCYVSV a t c t t c t c a t a c t c t c g t t c g g c c t g c t g g c t c t t c c t c c a g t a g c a g t a g a c a g a c a c c t a g a a g a g t a t g a g a g c a a g c c g g a c g a c c g a g a a g g a g g t c a t c g t c a t c t g t c t g t g g

3180 RDDDHHGHDDQRQDEAERHV AIVVVMPVIVLPLILSLPVD g c g a t g a c g a c c a c c a t g g g c a c g a t g a c c a g c g g c a a g a t g a g g c t g a g c g g c a c g t c g c g c t a c t g c t g g t g g t a c c c g t g c t a c t g g t c g c c g t t c t a c t c c g a c t c g c c g t g c a g c

3240 AHPCVVLHAAQHPLARAELH SVRTDYEVRGLVWERSGFKV c t c a c c c g t g t g t c g t a c t c c a c g c g g c c c a g c a c c c a c t c g c g a g a g c c g a a c t t c a c a g a g t g g g c a c a c a g c a t g a g g t g c g c c g g g t c g t g g g t g a g c g c t c t c g g c t t g a a g t g t

3300 NELGQVVCGVSFLPPLGRGL IFEPLNHTTDRKQRRKPPPQ a t g a a c t c g g g c a g g t t g t g t g t g g t g t c t c g t t t c t g c c g c c g c t t g g g c g g g g g c t g c t a c t t g a g c c c g t c c a a c a c a c a c c a c a g a g c a a a g a c g g c g g c g a a c c c g c c c c c g a c g

AfeI 3360 HLRGLTVQVGLRQRLHGAAL VEPPECYLDTETLTKMTCRE a c c t c c g g g g g c t c a c a g t a c a g g t c g g t c t c c g t c a g c g t c t t c a t g g t g c a g c g c t c g t g g a g g c c c c c g a g t g t c a t g t c c a g c c a g a g g c a g t c g c a g a a g t a c c a c g t c g c g a g c

3420 GTHEGLGLLQRHRLVQIGAP AGVFAEAEQLTMAKNLNTGR g c a c c c a c g a a g g c c t c g g c c t c c t g c a g c g t c a t c g c c t t g t t c a g a t t g g t g c c c c g g c g t g g g t g c t t c c g g a g c c g g a g g a c g t c g c a g t a g c g g a a c a a g t c t a a c c a c g g g g c c

3480 GVDELVDLLLDATCEVLKGG AHILKNVQKKVGGTFNEFTP g c g t g g a t g a g c t t g t t g a c c t g c t t c t t g a c g c c a c c t g t g a a g t t c t c a a a g g t g g g g c g c a c c t a c t c g a a c a a c t g g a c g a a g a a c t g c g g t g g a c a c t t c a a g a g t t t c c a c c c c

3540 VRHVLEGPGLCSEQGTVPVH DPVYEFAGAETRLLARHGDM t c a g g c a c g t a c t c g a a g g c c c c g g c c t c t g t t c t g a g c a g g g c a c g g t g c c c g t c c a t c a g t c c g t g c a t g a g c t t c c g g g g c c g g a g a c a a g a c t c g t c c c g t g c c a c g g g c a g g t a g

3600 LDQHREVVGLWLLRHSRGQE EILVTLNYAEPEEPVAPSLF t c g a t c a g c a c c g t g a g g t t g t a g g c c t c t g g c t c c t c a g g c a c a g c c g g g g a c a g g a a g a g c t a g t c g t g g c a c t c c a a c a t c c g g a g a c c g a g g a g t c c g t g t c g g c c c c t g t c c t t c

3660 DDLGVIVEHVVCTHHRHGLQ VVKTDNHFVYDTGVVTMPQL a c g a c c t t g g t g t c a t t g t g g a a c a c g t a g t c t g t a c c c a c c a c c g t c a t g g g c t g c a g g t g c t g g a a c c a c a g t a a c a c c t t g t g c a t c a g a c a t g g g t g g t g g c a g t a c c c g a c g t c c

3720 GFSLPRRLPGLQGLRDDHHG SEAERPPQWSQLPEAIVVMA g a t t c a g c c t c c c g c g g c g g c t g c c a g g a c t g c a g g g g c t c c g c g a t g a c c a c c a t g g c a c t a a g t c g g a g g g c g c c g c c g a c g g t c c t g a c g t c c c c g a g g c g c t a c t g g t g g t a c c g t

3780 KPLDQAEALTRDVDAAATTG FRQILSFGQGTVNISRGGSA a a c c t c t g g a t c a g g c t g a a g c c c t g a c c c g t g a c g t t g a t g c t g c g g c c a c c a c t g g c a t t g g a g a c c t a g t c c g a c t t c g g g a c t g g g c a c t g c a a c t a c g a c g c c g g t g g t g a c c g t

3840 KAS* FSRLPEFARLVPNERYTFFI a a g c t t c g t a g c g g c t c g a a g g c t c g c a g t a c g g g g t t t t c g c g g t a g g t g a a g a a g a t g t t c g a a g c a t c g c c g a g c t t c c g a g c g t c a t g c c c c a a a a g c g c c a t c c a c t t c t t c t a c

3900 GPNPVPSGGYSVELLMQGRT c c g g g g t t g g g c a c g g g g g a c c c c c c g t a g g a g a c c t c c a g a a g c a t c t g g c c c c g t g t c g g c c c c a a c c c g t g c c c c c t g g g g g g c a t c c t c t g g a g g t c t t c g t a g a c c g g g g c a c a g

3960 AQPGTVCQLQAGFKTVKCPV g c c t g g g g g c c a g t g a c a c a c t g g a g c t g c g c c c c a a a c t t c g t c a c t t t a c a c g g g a c g c g g a c c c c c g g t c a c t g t g t g a c c t c g a c g c g g g g t t t g a a g c a g t g a a a t g t g c c c t g c

4020 GNLTVRVDEQSGTDLHTGHI c c g t t g a g g g t c a c c c g c a c g t c c t c c t g g g a g c c c g t g t c c a g g t g g g t g c c g t g g a t g g g c a a c t c c c a g t g g g c g t g c a g g a g g a c c c t c g g g c a c a g g t c c a c c c a c g g c a c c t a c

4080 TLTTGGAQPGQQPEVSLPKP g t c a g t g t g g t g c c g c c c g c c t g c g g t c c c t g c t g c g g c t c c a c a c t g a g a g g c t t g g g c c a g t c a c a c c a c g g c g g g c g g a c g c c a g g g a c g a c g c c g a g g t g t g a c t c t c c g a a c c c g

4140 QQFTFQVNPPSRGLKGFVDV t g t t g g a a g g t g a a c t g g a c a t t g g g a g g c g a a c g g c c c a g t t t c c c g a a g a c g t c c a c c a c a a c c t t c c a c t t g a c c t g t a a c c c t c c g c t t g c c g g g t c a a a g g g c t t c t g c a g g t g g

4200 EVGGTFPTEAAEIVCVIRTS t c g a c a c c c c c c g t g a a a g g c g t c t c c g c a g c c t c g a t c a c a c a c a c g a t c c g g g t g g a c a g c t g t g g g g g g c a c t t t c c g c a g a g g c g t c g g a g c t a g t g t g t g t g c t a g g c c c a c c t g

FseI 4260 VSYREPQFSCNRGAVSIRQI a c g g a g t a a c g t t c c g g c t g a a a g g a g c a g t t c c g g c c g g c c a c a g a g a t c c t c t g g a t g t g c c t c a t t g c a a g g c c g a c t t t c c t c g t c a a g g c c g g c c g g t g t c t c t a g g a g a c c t a c

4320 DGAQVGLNSGLITIRIGGGL t c c c c t g c t t g g a c g c c c a a a t t g g a c c c c a g g a t g g t g a t g c g g a t g c c c c c a c c c a g g a g g g g a c g a a c c t g c g g g t t t a a c c t g g g g t c c t a c c a c t a c g c c t a c g g g g g t g g g t c c

4380 PGTEPQIRTIVPPPCESTTN g g g c c c g t c t c a g g c t g g a t c c t g g t g a t g a c g g g c g g c g g g c a c t c g g a g g t g g t g t t g c c c g g g c a g a g t c c g a c c t a g g a c c a c t a c t g c c c g c c g c c c g t g a g c c t c c a c c a c a a c

4440 CLAEYVCRSQGGCWACRYDP c a c a g g g c c t c a t a c a c g c a c c t g c t c t g g c c c c c g c a c c a c g c a c a c c t g t a g t c g g g g g t g t c c c g g a g t a t g t g c g t g g a c g a g a c c g g g g g c g t g g t g c g t g t g g a c a t c a g c c c c

4500 NAARCLSCDSRGFSCNYLTV t t a g c g g c c c g g c a c a g g c t g c a g t c g c t g c g g c c a a a g g a g c a g t t g t a g a g g g t c a c a a a t c g c c g g g c c g t g t c c g a c g t c a g c g a c g c c g g t t t c c t c g t c a a c a t c t c c c a g t g t

4560 MELAVDILAVRLDVEVQGQR HLKSDINKGYSKVYLHLPLT t g g a g c t t g c t g t c g a t a t t c t t g c c g t a a g a c t t g a c g t a g a g g t g c a g g g g c a g c g t c a c c t c g a a c g a c a g c t a t a a g a a c g g c a t t c t g a a c t g c a t c t c c a c g t c c c c g t c g c a g

4620 LVGIVGQLWGPKGEGPRFLH ENADHSLKPTRFAFTGSEQM t c g t t g g c a t c g t g g g a c a g c t t t g g g g t c c g a a a g g c g a a g g t c c c a g a t t c c t g c a t g a g c a a c c g t a g c a c c c t g t c g a a a c c c c a g g c t t t c c g c t t c c a g g g t c t a a g g a c g t a c

4680 GHRLHELEQVTAHVQGGTLH TVPEMFKLLDSGVHLSSGKV g t c a c c g g c t c c a t g a a c t t g a g c a a g t c a c t g c c c a c g t g c a g g g a g g a a c c c t t c a c g c a g t g g c c g a g g t a c t t g a a c t c g t t c a g t g a c g g g t g c a c g t c c c t c c t t g g g a a g t g c

4740 GVQVLALEVHICLVVHGDHQ TDLNKGQFNVDTEHNMPIVL g t g t c c a g g t t c t t g c c c t g g a a g t t c a c a t c t g t c t c g t g g t t c a t g g g g a t c a c c a g g c a c a g g t c c a a g a a c g g g a c c t t c a a g t g t a g a c a g a g c a c c a a g t a c c c c t a g t g g t c c

PshAI 4800 GAGSQELGTAVLHVGTDDAV PSPGLFQPCSDEMHARVIGD g g g c t g g g t c c c a g g a a c t g g g g a c a g c t g t c c t c c a t g t g g g c a c g g a c g a t g c c g t c c c c c g a c c c a g g g t c c t t g a c c c c t g t c g a c a g g a g g t a c a c c c g t g c c t g c t a c g g c a g g

4860 LRVGRSLPALVVAQVPLAGP EPNPSAERCEHYRLDWQCTW t c a g g g t t g g g c g a a g c c t c c c g g c a c t c g t g g t a g c g c a g g t c c c a c t g g c a g g t c c a g a g t c c c a a c c c g c t t c g g a g g g c c g t g a g c a c c a t c g c g t c c a g g g t g a c c g t c c a g g t c

4920 AVAHAGDARQVLLQAHGLAA RNSVCSICPLNEELSMAQRC c g g t t g c t c a c g c a g g a g a t g c a c g g c a g g t t c t c c t c c a g g c t c a t g g c c t g g c g g c a g g c c a a c g a g t g c g t c c t c t a c g t g c c g t c c a a g a g g a g g t c c g a g t a c c g g a c c g c c g t c

4980 VVEGVLVGREEDVASSKEEL DYFPYQYSTLFINGRRLLLQ t c g t a g a a g g g g t a c t g g t a g g a c g t g a g g a a g a t g t t g c c t c g t c t a a g g a g g a g c t g g a g c a t c t t c c c c a t g a c c a t c c t g c a c t c c t t c t a c a a c g g a g c a g a t t c c t c c t c g a c c

5040 DGHGHVVLAWRCDGDAAWGV ITVAVHDQGPPTVPISSPSN a t g g t c a c g g c c a c g t g g t c c t g g c c t g g c g g t g t g a c g g g g a t g c t g c t t g g g g a g t t g t a c c a g t g c c g g t g c a c c a g g a c c g g a c c g c c a c a c t g c c c c t a c g a c g a a c c c c t c a a c

5100 ADDGLALHAGGVWRRLPKKA CIVAEGEVRAPHPPSEGFLC c a g a t g a c g g c c t c g c c c t c c a c g c g g g c g g g g t g t g g c g g c g a c t c c c c a a a a a g g c a c g t c t a c t g c c g g a g c g g g a g g t g c g c c c g c c c c a c a c c g c c g c t g a g g g g t t t t t c c g t g

5160 QQLVLLAQGREGADGQLHLP LLEDEESLAPLPSVTLQVEG a g c a a c t c g t c c t c c t c g c t c a g g g c a g g g a g g g g g c t g a c g g t c a g c t g c a c c t c c c c c t c g t t g a g c a g g a g g a g c g a g t c c c g t c c c t c c c c c g a c t g c c a g t c g a c g t g g a g g g g g

5220 LGPPAHVLWLGAGDGHAGLA QARRSMNQPQASTVAVCSKS t g g g c c c g c c g g c t c a t g t t c t g t g g c t g g g c g c t g g t g a c g g c c a c g c a g g a c t t g c t t a c c c g g g c g g c c g a g t a c a a g a c a c c g a c c c g c g a c c a c t g c c g g t g c g t c c t g a a c g a a

5280 SAPQPVAGLLGPRTLGLPGA RSWLWHSAEEARPCEAKRTC c g g c t c c a c a g c c a g t g g c t g g c c t c c t c g g c c c g c g g a c a c t c g g c c t t c c g g g t g c a t g c c g a g g t g t c g g t c a c c g a c c g g a g g a g c c g g g c g c c t g t g a g c c g g a a g g c c c a c g t a

5340 SSLDDAPAAVGVLGVAALGA RGEVVCWGCYPDQSDRCQTC c g t c c c t c g a c g a c g c a c c a g c c g c a g t a g g g g t c c t g g g a g t c g c g g c a c t g g g t g c a g g c a g g g a g c t g c t g c g t g g t c g g c g t c a t c c c c a g g a c c c t c a g c g c c g t g a c c c a c g t c

5400 GRVAQALLHRQPEHLVLGHG TPYSLCEQVPLRFVKDQTMA g t c g g g t a g c t c a g g c a c t c c t g c a c c g g c a g c c g g a a c a c c t t g t c c t g g g t c a t g g c g c a g c c c a t c g a g t c c g t g a g g a c g t g g c c g t c g g c c t t g t g g a a c a g g a c c c a g t a c c g c

5460 VQAAQVSRQYQVALDSLVYL YLSGLDGSLVLDRKVRKNIE t a c a g g c t g c c c a g g t c t c c a g a c a g t a c c a g g t c g c g c t t g a c t c t c t t g t t t a t c t c c a t g t c c g a c g g g t c c a g a g g t c t g t c a t g g t c c a g c g c g a a c t g a g a g a a c a a a t a g a g g

5520 HKDRVVL* VLISDYESSTGDPTLYVKLI a c a a g g a t a g a g t c g t a c t c t g a g g a g g t g c c a t c t g g g g t g a g g t a c a c c t t g a g g a t c t g t t c c t a t c t c a g c a t g a g a c t c c t c c a c g g t a g a c c c c a c t c c a t g t g g a a c t c c t a g

5580 RGDSTGLFAVTHNNEAAVTV c g g c c a t c a g a g g t g c c c a g a a a a g c a a c a g t g t g g t t a t t t t c a g c a g c c a c g g t g a c a g c c g g t a g t c t c c a c g g g t c t t t t c g t t g t c a c a c c a a t a a a a g t c g t c g g t g c c a c t g t

5640 ATLNLGGRQLVATGRLGDRS g c c g t g a g g t t c a g g c c t c c a c g c t g c a g c a c g g c t g t g c c t c t g a g c c c g t c g c g g c t g c g g c a c t c c a a g t c c g g a g g t g c g a c g t c g t g c c g a c a c g g a g a c t c g g g c a g c g c c g a c

5700 GLPYPLHESGCPFSKSSGPA c c c a g c g g g t a g g g c a g g t g c t c c g a g c c a c a t g g g a a g c t c t t g c t g g a g c c c g g c g c g g g g t c g c c c a t c c c g t c c a c g a g g c t c g g t g t a c c c t t c g a g a a c g a c c t c g g g c c g c g c

5760 HGGCQIDGHFPKYFIDRAER t g g c c g c c g c a c t g g a t a t c g c c g t g g a a g g g c t t g t a g a a g a t g t c a c g g g c c t c c c g g a c c g g c g g c g t g a c c t a t a g c g g c a c c t t c c c g a a c a t c t t c t a c a g t g c c c g g a g g g c c

5820 TGTYCANRNAEMKAHVKDLP g t g c c t g t g t a a c a g g c g t t g c g g t t g g c c t c c a t c t t g g c g t g c a c c t t g t c c a g c g g g c a c g g a c a c a t t g t c c g c a a c g c c a a c c g g a g g t a g a a c c g c a c g t g g a a c a g g t c g c c c

5880 FLCLGAGPGGSSRSDRSFVA a a c a g g c a g a g g c c c g c a c c g g g c c c c c c a c t g c t c c g g c t g t c t c t g c t g a a g a c a g c a t t g t c c g t c t c c g g g c g t g g c c c g g g g g g t g a c g a g g c c g a c a g a g a c g a c t t c t g t c g t

5940 YLVRGSGPAAVSAALCTGFA t a t a g c a c c c t g c c a g a g c c a g g c g c a g c c a c g g a g g c g g c c a g g c a g g t g c c a a a g g c a a t a t c g t g g g a c g g t c t c g g t c c g c g t c g g t g c c t c c g c c g g t c c g t c c a c g g t t t c c g t

6000 AAHIDPDRCQLDMELYSYYN g c g g c g t g g a t g t c g g g g t c c c g g c a c t g c a g g t c c a t c t c c a g g t a g g a g t a g t a g t t g c g c c g c a c c t a c a g c c c c a g g g c c g t g a c g t c c a g g t a g a g g t c c a t c c t c a t c a t c a a c

6060 PDERCMRALLTRNRAPHKDQ g g g t c t t c t c t g c a c a t g c g t g c c a g c a g c g t g c g g t t c c g g g c c g g g t g c t t g t c c t g c c c c a g a a g a g a c g t g t a c g c a c g g t c g t c g c a c g c c a a g g c c c g g c c c a c g a a c a g g a c g

6120 QNFVFFVYPGDEFAAVFQQT t g g t t g a a g a c a a a g a a g a c g t a g g g g c c g t c c t c g a a g g c c g c c a c g a a c t g c t g t g t g a c c a a c t t c t g t t t c t t c t g c a t c c c c g g c a g g a g c t t c c g g c g g t g c t t g a c g a c a c a c

6180 NTSLYGAKYTAHDTYAEFAE t t g g t g g a c a g g t a g c c g g c c t t g t a g g t g g c g t g g t c c g t g t a g g c t t c a a a g g c c t c c a a c c a c c t g t c c a t c g g c c g g a a c a t c c a c c g c a c c a g g c a c a t c c g a a g t t t c c g g a g g

6240 RSDTRDLLRTSVIIGNDHPG c t g c t g t c a g t c c g g t c c a a c a g c c g a g t g c t c a c g a t g a t g c c g t t g t c g t g t g g c c c a g a c g a c a g t c a g g c c a g g t t g t c g g c t c a c g a g t g c t a c t a c g g c a a c a g c a c a c c g g g t

6300 NGKGVFLVRDGGPGTSSVLG t t g c c t t t g c c c a c a a a c a g c a c g c g g t c a c c a c c a g g a c c c g t g g a g c t c a c c a g c c c c a a c g g a a a c g g g t g t t t g t c g t g c g c c a g t g g t g g t c c t g g g c a c c t c g a g t g g t c g g g g

6360 VTAVGEDNSAVFSKEGSGDE a c t g t g g c c a c g c c c t c a t c a t t g c t g g c c a c g a a a g a c t t c t c c c c g c t g c c g t c c t c g t g a c a c c g g t g c g g g a g t a g t a a c g a c c g g t g c t t t c t g a a g a g g g g c g a c g g c a g g a g c

6420 YFLRLSINSLARLACIGKFL t a g a a c a g g c g g a g g g a g a t g t t g c t c a g g g c g c g c a g a g c g c a g a t g c c c t t g a a g a g g a t c t t g t c c g c c t c c c t c t a c a a c g a g t c c c g c g c g t c t c g c g t c t a c g g g a a c t t c t c c

6480 SGCEVLRKRPPDLLLLQNVN c t g c c g c a c t c c a c c a g g c g c t t c c t g g g a g g g t c g a g c a g c a g c a g c t g g t t g a c a t t g g a c g g c g t g a g g t g g t c c g c g a a g g a c c c t c c c a g c t c g t c g t c g t c g a c c a a c t g t a a c

6540 DTMEAEHCQSAEIPPTCKKN t c a g t c a t c t c a g c c t c a t g g c a c t g g c t g g c c t c g a t g g g c g g c g t g c a c t t c t t g t t g a g t c a g t a g a g t c g g a g t a c c g t g a c c g a c c g g a g c t a c c c g c c g c a c g t g a a g a a c a a c

6600 DLAPGTAVQQELQLKADLQY t c c a g g g c c g g g c c c g t g g c c a c c t g c t g c t c c a g c t g c a g c t t c g c a t c c a g c t g g t a g a g g t c c c g g c c c g g g c a c c g g t g g a c g a c g a g g t c g a c g t c g a a g c g t a g g t c g a c c a t c

6660 LANVAGLYVVGSAEDVALHN a g g g c a t t c a c c g c c c c c a g g t a c a c c a c g c c t g a g g c c t c a t c c a c a g c c a g g t g g t t c t c c c g t a a g t g g c g g g g g t c c a t g t g g t g c g g a c t c c g g a g t a g g t g t c g g t c c a c c a a g

6720 LEKESRFFDLKRPRLSAGAG a g c t c t t t c t c g c t g c g g a a g a a g t c c a g c t t g c g g g g c c t c a g g c t g g c a c c t g c g c c c t c g a g a a a g a g c g a c g c c t t c t t c a g g t c g a a c g c c c c g g a g t c c g a c c g t g g a c g c g g g

6780 attB1 MVGLDGP LLGLLTLAWLQLAMTPRSPG a g c a g g c c c a g c a g g g t c a g g g c c c a g a g c t g c a g t g c c a t g g t g g g c c t t g a c g g c c c t t c g t c c g g g t c g t c c c a g t c c c g g g t c t c g a c g t c a c g g t a c c a c c c g g a a c t g c c g g g a

SalI 6840 attB1 SAFLYKLVDICRIPPHWTSR EAKKYLSTSIQLIGGCQVLR t c t g c t t t t t t g t a c a a a c t t g t t g a t a t c t g c a g a a t t c c a c c a c a c t g g a c t a g t c g a a g a c g a a a a a a c a t g t t t g a a c a a c t a t a g a c g t c t t a a g g t g g t g t g a c c t g a t c a g c t

6900 hPGK promoter RTGGSPWGERSVIRSTREPT RVPPDGQPSLDTIRDVLSGV c g a a c c g g t g g a t c c c c c t g g g g a g a g a g g t c g g t g a t t c g g t c a a c g a g g g a g c c g a c t g c t t g g c c a c c t a g g g g g a c c c c t c t c t c c a g c c a c t a a g c c a g t t g c t c c c t c g g c t g a

6960 hPGK promoter G A A C G T C T T A C G C C T T G T G hPGK-F ADVRSGGLQNAEHRAGRNRA ASTREPPKCFASCRAPLFLA g c c g a c g t g c g c t c c g g a g g c t t g c a g a a t g c g g a a c a c c g c g c g g g c a g g a a c a g g g c c c g g c t g c a c g c g a g g c c t c c g a a c g t c t t a c g c c t t g t g g c g c g c c c g t c c t t g t c c c g g

AscI 7020 hPGK promoter HTTAPHPASRTAPSRPLLSA WVVAGCGAERVAGERGSSEA c a c a c t a c c g c c c c a c a c c c c g c c t c c c g c a c c g c c c c t t c c c g g c c g c t g c t c t c g g c g g t g t g a t g g c g g g g t g t g g g g c g g a g g g c g t g g c g g g g a a g g g c c g g c g a c g a g a g c c g c

7080 hPGK promoter RPAEQPLLATAHRGRRAAIA RGASCGSNAVAWRPRRAAM c g c c c t g c t g a g c a g c c g c t a t t g g c c a c a g c c c a t c g c g g t c g g c g c g c t g c c a t t g c t g c g g g a c g a c t c g t c g g c g a t a a c c g g t g t c g g g t a g c g c c a g c c g c g c g a c g g t a a c g a

7140 (in frame with gp41 peptide) *HSTRSGNTVD hPGK promoter PWRCPSARVLVRRAASVCHV *HSTRSGNTVD c c c t g g c g c t g t c c g t c t g c g a g g g t a c t a g t g a g a c g t g c g g c t t c c g t t t g t c a c g t c g g g a c c g c g a c a g g c a g a c g c t c c c a t g a t c a c t c t g c a c g c c g a a g g c a a a c a g t g c a g

7200 (in frame with gp41 peptide) PVGRVALFRGSKPASCSADG hPGK promoter RHAANRKEPSRLRGGAGSVA PVGRVALFRGSKPASCSADG c g g c a c g c c g c g a a c c g c a a g g a a c c t t c c c g a c t t a g g g g c g g a g c a g g a a g c g t c g c c g c c g t g c g g c g c t t g g c g t t c c t t g g a a g g g c t g a a t c c c c g c c t c g t c c t t c g c a g c g g

7260 (in frame with gp41 peptide) PPGCPYRRLDPHRQSRVHLI hPGK promoter GGPTRVAAKIRVTLRTDVKN PPGCPYRRLDPHRQSRVHLI g g g g g g c c c a c a a g g g t a g c g g c g a a g a t c c g g g t g a c g c t g c g a a c g g a c g t g a a g a a t c c c c c c g g g t g t t c c c a t c g c c g c t t c t a g g c c c a c t g c g a c g c t t g c c t g c a c t t c t t a

7320 (in frame with gp41 peptide) HSVWPRRRQTERFWAWLLRT hPGK promoter VRDPGSAPLRFPEPRPEQPR HSVWPRRRQTERFWAWLLRT g t g c g a g a c c c a g g g t c g g c g c c g c t g c g t t t c c c g g a a c c a c g c c c a g a g c a g c c g c g t c a c g c t c t g g g t c c c a g c c g c g g c g a c g c a a a g g g c c t t g g t g c g g g t c t c g t c g g c g c a

7380 (in frame with gp41 peptide) GACVWPQRPFPAVGVGHFEI hPGK promoter PCANPGLPWKRRNPNPVEFD GACVWPQRPFPAVGVGHFEI c c c t g c g c a a a c c c a g g g c t g c c t t g g a a a a g g c g c a a c c c c a a c c c c g t g g a a t t c g a t g g g a c g c g t t t g g g t c c c g a c g g a a c c t t t t c c g c g t t g g g g t t g g g g c a c c t t a a g c t a

BspDI*ClaI*PaeR7IXhoI 7440 (in frame with gp41 peptide) DLKELSTEHDIFNQIKTIQK cPPT/CTS IKLLEASLVIDKILNFCNLF DLKELSTEHDIFNQIKTIQK a t c a a g c t t c t c g a g g c t a g t c t c g t g a t c g a t a a a a t t t t g a a t t t t t g t a a t t t g t t t t a g t t c g a a g a g c t c c g a t c a g a g c a c t a g c t a t t t t a a a a c t t a a a a a c a t t a a a c a a a

7500 (in frame with gp41 peptide) QLEKTQIDTAIIDVIREGAS cPPT/CTS L* QLEKTQIDTAIIDVIREGAS t t g t a a t t c t t t a g t t t g t a t g t c t g t t g c t a t t a t g t c t a c t a t t c t t t c c c c t g c a c t a a c a t t a a g a a a t c a a a c a t a c a g a c a a c g a t a a t a c a g a t g a t a a g a a a g g g g a c g t g a

HpaI 7560 (in frame with gp41 peptide) YGGIGGKRKFTLRYRRSGNV cPPT/CTS YGGIGGKRKFTLRYRRSGNV g t a c c c c c c a a t c c c c c c t t t t c t t t t a a a a g t t a a c c g a t a c c g t c g a g a t c c g t t c a c c a t g g g g g g t t a g g g g g g a a a a g a a a a t t t t c a a t t g g c t a t g g c a g c t c t a g g c a a g t g

7620 (in frame with gp41 peptide) LRISRDRDREGGEEEIGEPR LRISRDRDREGGEEEIGEPR t a a t c g a a t g g a t c t g t c t c t g t c t c t c t c t c c a c c t t c t t c t t c t a t t c c t t c g g g c c t a t t a g c t t a c c t a g a c a g a g a c a g a g a g a g a g g t g g a a g a a g a a g a t a a g g a a g c c c g g a

7680 (in frame with gp41 peptide) DPGRPTPLHTQFSLPSYGQR DPGRPTPLHTQFSLPSYGQR g t c g g g t c c c c t c g g g g t t g g g a g g t g g g t c t g a a a c g a t a a t g g t g a a t a t c c c t g c c t c a g c c c a g g g g a g c c c c a a c c c t c c a c c c a g a c t t t g c t a t t a c c a c t t a t a g g g a c g g a

7740 (in frame with gp41 peptide) VRNVISLVAFVIRLGVLGGV VRNVISLVAFVIRLGVLGGV a a c t c t a t t c a c t a t a g a a a g t a c a g c a a a a a c t a t t c t t a a a c c t a c c a a g c c t c c t a c t t g a g a t a a g t g a t a t c t t t c a t g t c g t t t t t g a t a a g a a t t t g g a t g g t t c g g a g g a t g

7800 (in frame with gp41 peptide) IMIFLKIYWLWNTINFWNW 15 L gp41 peptide IMIFLKIYWLWNTINFWNWL t a t c a t t a t g a a t a a t t t t a t a t a c c a c a g c c a a t t t g t t a t g t t a a a c c a a t t c c a c a a a t a g t a a t a c t t a t t a a a a t a t a t g g t g t c g g t t a a a c a a t a c a a t t t g g t t a a g g t g t t

7860 10 5 1 SAWKDLELLEQENK gp41 peptide SAWKDLELLEQENKEQQNQS a c t t g c c c a t t t a t c t a a t t c c a a t a a t t c t t g t t c a t t c t t t t c t t g c t g g t t t t g c g a t g a a c g g g t a a a t a g a t t a a g g t t a t t a a g a a c a a g t a a g a a a a g a a c g a c c a a a a c g c t

7920 EEILSHILSTYNNIERDWEM t t c t t c a a t t a a g g a g t g t a t t a a g c t t g t g t a a t t g t t a a t t t c t c t g t c c c a c t c c a t a a g a a g t t a a t t c c t c a c a t a a t t c g a a c a c a t t a a c a a t t a a a g a g a c a g g g t g a g g t a

7980 WTTHNWIQELSKNSWSANWP c c a g g t c g t g t g a t t c c a a a t c t g t t c c a g a g a t t t a t t a c t c c a a c t a g c a t t c c a a g g g g t c c a g c a c a c t a a g g t t t a g a c a a g g t c t c t a a a t a a t g a g g t t g a t c g t a a g g t t c c

8040 RRE VATTCILKGSCGWIGLLQQD c a c a g c a g t g g t g c a a a t g a g t t t t c c a g a g c a a c c c c a a a t c c c c a g g a g c t g t t g a t c g t g t c g t c a c c a c g t t t a c t c a a a a g g t c t c g t t g g g g t t t a g g g g t c c t c g a c a a c t a g

8100 RRE KLYREVALIRAQLQKIGWVT c t t t a g g t a t c t t t c c a c a g c c a g g a t t c t t g c c t g g a g c t g c t t g a t g c c c c a g a c t g t g a a a t c c a t a g a a a g g t g t c g g t c c t a a g a a c g g a c c t c g a c g a a c t a c g g g g t c t g a c a

BbvCI 8160 RRE LQLLHQQAEIARLLNNQQQV g a g t t g c a a c a g a t g c t g t t g c g c c t c a a t a g c c c t c a g c a a a t t g t t c t g c t g c t g c a c c t c a a c g t t g t c t a c g a c a a c g c g g a g t t a t c g g g a g t c g t t t a a c a a g a c g a c g a c g t g

8220 RRE IGSLLQRAQVTLTMSAAGMT t a t a c c a g a c a a t a a t t g t c t g g c c t g t a c c g t c a g c g t c a t t g a c g c t g c g c c c a t a g t a t a t g g t c t g t t a t t a a c a g a c c g g a c a t g g c a g t c g c a g t a a c t g c g a c g c g g g t a t c a

8280 RRE SGAAGLFGLFLAGIGVARKE g c t t c c t g c t g c t c c c a a g a a c c c a a g g a a c a a a g c t c c t a t t c c c a c t g c t c t t t t t t c c g a a g g a c g a c g a g g g t t c t t g g g t t c c t t g t t t c g a g g a t a a g g g t g a c g a g a a a a a a g

8340 RQVVRRKAKTPAVGLPEIKV t c t c t g c a c c a c t c t t c t c t t t g c c t t g g t g g g t g c t a c t c c t a a t g g t t c a a t t t t t a c a g a g a c g t g g t g a g a a g a g a a a c g g a a c c a c c c a c g a t g a g g a t t a c c a a g t t a a a a a t g

MfeI 8400 VKYKYLESRWNDRM t a c t t t a t a t t t a t a t a a t t c a c t t c t c c a a t t g t c c c t c a t a t c t c c t c c t c c a g g t c t a t g a a a t a t a a a t a t a t t a a g t g a a g a g g t t a a c a g g g a g t a t a g a g g a g g a g g t c c a g a

8460 g a a g a t c a g c g g c c g c t t g c t g t g c g g t g g t c t t a c t t t t g t t t t g c t c t t c c t c t a t c t c t t c t a g t c g c c g g c g a a c g a c a c g c c a c c a g a a t g a a a a c a a a a c g a g a a g g a g a t a g a

8520 t g t c t a a a g c t t c c t t g g t g t c t t t t a t c t c t a t c c t t t g a t g c a c a c a a t a g a g g g t t g a c a g a t t t c g a a g g a a c c a c a g a a a a t a g a g a t a g g a a a c t a c g t g t g t t a t c t c c c a a c

8580 c t a c t g t a t t a t a t a a t g a t c t a a g t t c t t c t g a t c c t g t c t g a a g g g a t g g t t g t a g c t g a t g a c a t a a t a t a t t a c t a g a t t c a a g a a g a c t a g g a c a g a c t t c c c t a c c a a c a t c g a

8640 g t c c c a g t a t t t g t c t a c a g c c t t c t g a t g t t t c t a a c a g g c c a g g a t t a a c t g c g a a t c c a g g g t c a t a a a c a g a t g t c g g a a g a c t a c a a a g a t t g t c c g g t c c t a a t t g a c g c t t a g

8700 g t t c t a g c t c c c t g c t t g c c c a t a c t a t a t g t t t t a a t t t a t a t t t t t t c t t t c c c c c t g c a a g a t c g a g g g a c g a a c g g g t a t g a t a t a c a a a a t t a a a t a t a a a a a a g a a a g g g g g a c

8760 HIV-1 Ψ g c c t t a a c c g a a t t t t t t c c c a t c g c g a t c t a a t t c t c c c c c g c t t a a t a c t g a c g c t c t c g g a a t t g g c t t a a a a a a g g g t a g c g c t a g a t t a a g a g g g g g c g a a t t a t g a c t g c g a g a

8820 HIV-1 Ψ c g c a c c c a t c t c t c t c c t t c t a g c c t c c g c t a g t c a a a a t t t t t g g c g t a c t c a c c a g t c g c g t g g g t a g a g a g a g g a a g a t c g g a g g c g a t c a g t t t t a a a a a c c g c a t g a g t g g t c a g

8880 HIV-1 Ψ g c c g c c c c t c g c c t c t t g c c g t g c g c g c t t c a g c a a g c c g a g t c c t g c g t c g a g a g a g c t c g g c g g g g a g c g g a g a a c g g c a c g c g c g a a g t c g t t c g g c t c a g g a c g c a g c t c t c t c g a

8940 5' LTR (truncated) c c t c t g g t t t c c c t t t c g c t t t c a a g t c c c t g t t c g g g c g c c a c t g c t a g a g a t t t t c c a g g a g a c c a a a g g g a a a g c g a a a g t t c a g g g a c a a g c c c g c g g t g a c g a t c t c t a a a a g g t

9000 5' LTR (truncated) c a c t g a c t a a a a g g g t c t g a g g g a t c t c t a g t t a c c a g a g t c a c a c a a c a g a c g g g c a c a g t g a c t g a t t t t c c c a g a c t c c c t a g a g a t c a a t g g t c t c a g t g t g t t g t c t g c c c g t g t

9060 5' LTR (truncated) c a c t a c t t g a a g c a c t c a a g g c a a g c t t t a t t g a g g c t t a a g c a g t g g g t t c c c t a g t t a g t g a t g a a c t t c g t g a g t t c c g t t c g a a a t a a c t c c g a a t t c g t c a c c c a a g g g a t c a a t

9120 5' LTR (truncated) RSV promoter g c c a g a g a g c t c c c a g g c t c a g a t c t g g t c t a a c c a g a g a g a c c c g t t t a t g t a t c g a g c c g g t c t c t c g a g g g t c c g a g t c t a g a c c a g a t t g g t c t c t c t g g g c a a a t a c a t a g c t c g

9180 RSV promoter t a g g c a c t t a a a t a c a a t a t c t c t g c a a t g c g g c a a t t c a g t g g t t c g t c c a a t c c a t g t a t c c g t g a a t t t a t g t t a t a g a g a c g t t a c g c c g t t a a g t c a c c a a g c a g g t t a g g t a c a

9240 RSV promoter c a g a c c c g t c t g t t g c c t t c c t a a t a a g g c a c g a t c g t a c c a c c t t a c t t c c a c c a a t c g g t c t g g g c a g a c a a c g g a a g g a t t a t t c c g t g c t a g c a t g g t g g a a t g a a g g t g g t t a g c

9300 RSV promoter g c a t g c a c g g t g c t t t t t c t c t c c t t g t a a g g c a t g t t g c t a a c t c a t c g t t a c c a t g t t c g t a c g t g c c a c g a a a a a g a g a g g a a c a t t c c g t a c a a c g a t t g a g t a g c a a t g g t a c a a

9360 RSV promoter T3 promoter G T3 g c a a g a c t a c a a g a g t a t t g c a t a a g a c t a c a t t a a g c t t g c a g c t c c a g c t t t t g t t c c c g t t c t g a t g t t c t c a t a a c g t a t t c t g a t g t a a t t c g a a c g t c g a g g t c g a a a a c a a g g

9420 T3 promoter M13 rev G A A A T C A C T C C C A A T T A A C G T3 C A G T A T C G A C A A A G G A C M13 Reverse G G A C A C A C T M13/pUC Reverse c t t t a g t g a g g g t t a a t t g c g c g c t t g g c g t a a t c a t g g t c a t a g c t g t t t c c t g t g t g a g a a a t c a c t c c c a a t t a a c g c g c g a a c c g c a t t a g t a c c a g t a t c g a c a a a g g a c a c a c t

9480 -10 -35 lac operator lac promoter T T A A C A A T A G G C G A M13/pUC Reverse a a t t g t t a t c c g c t c a c a a t t c c a c a c a a c a t a c g a g c c g g a a g c a t a a a g t g t a a a g c c t t a a c a a t a g g c g a g t g t t a a g g t g t g t t g t a t g c t c g g c c t t c g t a t t t c a c a t t t c g g

9540 CAP binding site t g g g g t g c c t a a t g a g t g a g c t a a c t c a c a t t a a t t g c g t t g c g c t c a c t g c c c g c t t t c a c c c c a c g g a t t a c t c a c t c g a t t g a g t g t a a t t a a c g c a a c g c g a g t g a c g g g c g a a a g

9600 c a g t c g g g a a a c c t g t c g t g c c a g c t g c a t t a a t g a a t c g g c c a a c g c g c g g g g a g a g g c g t c a g c c c t t t g g a c a g c a c g g t c g a c g t a a t t a c t t a g c c g g t t g c g c g c c c c t c t c c g

9660 G A G C G A G T G A C T G A G C G A L4440 g g t t t g c g t a t t g g g c g c t c t t c c g c t t c c t c g c t c a c t g a c t c g c t g c g c t c g g t c g t t c c a a a c g c a t a a c c c g c g a g a a g g c g a a g g a g c g a g t g a c t g a g c g a c g c g a g c c a g c a a

9720 c g g c t g c g g c g a g c g g t a t c a g c t c a c t c a a a g g c g g t a a t a c g g t t a t c c a c a g a a t c a g c c g a c g c c g c t c g c c a t a g t c g a g t g a g t t t c c g c c a t t a t g c c a a t a g g t g t c t t a g t

PciI 9780 g g g g a t a a c g c a g g a a a g a a c a t g t g a g c a a a a g g c c a g c a a a a g g c c a g g a a c c g t a a a c c c c t a t t g c g t c c t t t c t t g t a c a c t c g t t t t c c g g t c g t t t t c c g g t c c t t g g c a t t t

9840 ori a a g g c c g c g t t g c t g g c g t t t t t c c a t a g g c t c c g c c c c c c t g a c g a g c a t c a c a a a a a t t t c c g g c g c a a c g a c c g c a a a a a g g t a t c c g a g g c g g g g g g a c t g c t c g t a g t g t t t t t a

9900 ori T T T C T A T G G T C C G C A A A G G G pBR322ori-F c g a c g c t c a a g t c a g a g g t g g c g a a a c c c g a c a g g a c t a t a a a g a t a c c a g g c g t t t c c c g c t g c g a g t t c a g t c t c c a c c g c t t t g g g c t g t c c t g a t a t t t c t a t g g t c c g c a a a g g g

9960 ori c c t g g a a g c t c c c t c g t g c g c t c t c c t g t t c c g a c c c t g c c g c t t a c c g g a t a c c t g t c c g g a c c t t c g a g g g a g c a c g c g a g a g g a c a a g g c t g g g a c g g c g a a t g g c c t a t g g a c a g g

10,020 ori g c c t t t c t c c c t t c g g g a a g c g t g g c g c t t t c t c a t a g c t c a c g c t g t a g g t a t c t c a g t c g g a a a g a g g g a a g c c c t t c g c a c c g c g a a a g a g t a t c g a g t g c g a c a t c c a t a g a g t c a

10,080 ori t c g g t g t a g g t c g t t c g c t c c a a g c t g g g c t g t g t g c a c g a a c c c c c c g t t c a g c c c g a c a g c c a c a t c c a g c a a g c g a g g t t c g a c c c g a c a c a c g t g c t t g g g g g g c a a g t c g g g c t g

10,140 ori c g c t g c g c c t t a t c c g g t a a c t a t c g t c t t g a g t c c a a c c c g g t a a g a c a c g a c t t a t c g g c g a c g c g g a a t a g g c c a t t g a t a g c a g a a c t c a g g t t g g g c c a t t c t g t g c t g a a t a g c

10,200 ori c c a c t g g c a g c a g c c a c t g g t a a c a g g a t t a g c a g a g c g a g g t a t g t a g g c g g t g c t a c a g g t g a c c g t c g t c g g t g a c c a t t g t c c t a a t c g t c t c g c t c c a t a c a t c c g c c a c g a t g t

10,260 ori g a g t t c t t g a a g t g g t g g c c t a a c t a c g g c t a c a c t a g a a g a a c a g t a t t t g g t a t c t g c c t c a a g a a c t t c a c c a c c g g a t t g a t g c c g a t g t g a t c t t c t t g t c a t a a a c c a t a g a c g

10,320 ori g c t c t g c t g a a g c c a g t t a c c t t c g g a a a a a g a g t t g g t a g c t c t t g a t c c g g c a a a c a a c g a g a c g a c t t c g g t c a a t g g a a g c c t t t t t c t c a a c c a t c g a g a a c t a g g c c g t t t g t t

10,380 ori a c c a c c g c t g g t a g c g g t g g t t t t t t t g t t t g c a a g c a g c a g a t t a c g c g c a g a a a a a a a t g g t g g c g a c c a t c g c c a c c a a a a a a a c a a a c g t t c g t c g t c t a a t g c g c g t c t t t t t t t

10,440 ori g g a t c t c a a g a a g a t c c t t t g a t c t t t t c t a c g g g g t c t g a c g c t c a g t g g a a c g a a a a c c c t a g a g t t c t t c t a g g a a a c t a g a a a a g a t g c c c c a g a c t g c g a g t c a c c t t g c t t t t g

10,500 t c a c g t t a a g g g a t t t t g g t c a t g a g a t t a t c a a a a a g g a t c t t c a c c t a g a t c c t t t t a a g t g c a a t t c c c t a a a a c c a g t a c t c t a a t a g t t t t t c c t a g a a g t g g a t c t a g g a a a a t

10,560 AmpR a a t t a a a a a t g a a g t t t t a a a t c a a t c t a a a g t a t a t a t g a g t a a a c t t g g t c t g a c a g t t t a a t t t t t a c t t c a a a a t t t a g t t a g a t t t c a t a t a t a c t c a t t t g a a c c a g a c t g t c a

10,620 285 280 275 270 *WHKILSAGIEAIQRNREDM AmpR *WHKILSAGIEAIQRNREDM t a c c a a t g c t t a a t c a g t g a g g c a c c t a t c t c a g c g a t c t g t c t a t t t c g t t c a t c c a t a a t g g t t a c g a a t t a g t c a c t c c g t g g a t a g a g t c g c t a g a c a g a t a a a g c a a g t a g g t a t

10,680 265 260 255 250 TAQSGTTYIVVIRSPKGDPG AmpR TAQSGTTYIVVIRSPKGDPG g t t g c c t g a c t c c c c g t c g t g t a g a t a a c t a c g a t a c g g g a g g g c t t a c c a t c t g g c c c c c a a c g g a c t g a g g g g c a g c a c a t c t a t t g a t g c t a t g c c c t c c c g a a t g g t a g a c c g g g g

10,740 245 240 235 230 LAAIIGRSGREGAGSKDAIF AmpR LAAIIGRSGREGAGSKDAIF a g t g c t g c a a t g a t a c c g c g a g a c c c a c g c t c a c c g g c t c c a g a t t t a t c a g c a a t a a a c t c a c g a c g t t a c t a t g g c g c t c t g g g t g c g a g t g g c c g a g g t c t a a a t a g t c g t t a t t t g

10,800 225 220 215 210 WGAPLASRLLPGAVKDAEMW AmpR WGAPLASRLLPGAVKDAEMW c a g c c a g c c g g a a g g g c c g a g c g c a g a a g t g g t c c t g c a a c t t t a t c c g c c t c c a t c c a g g t c g g t c g g c c t t c c c g g c t c g c g t c t t c a c c a g g a c g t t g a a a t a g g c g g a g g t a g g t c

10,860 205 200 195 190 DILQQRSALTLLEGTLLKRL AmpR DILQQRSALTLLEGTLLKRL t c t a t t a a t t g t t g c c g g g a a g c t a g a g t a a g t a g t t c g c c a g t t a a t a g t t t g c g c a a c a g a t a a t t a a c a a c g g c c c t t c g a t c t c a t t c a t c a a g c g g t c a a t t a t c a a a c g c g t t g

10,920 185 180 175 170 TTAMAVPMTTDREDNPIAEN AmpR TTAMAVPMTTDREDNPIAEN g t t g t t g c c a t t g c t a c a g g c a t c g t g g t g t c a c g c t c g t c g t t t g g t a t g g c t t c a t t c c a a c a a c g g t a a c g a t g t c c g t a g c a c c a c a g t g c g a g c a g c a a a c c a t a c c g a a g t a a g

10,980 165 160 155 150 LEPEWRDLRTVHDGMNHLFA AmpR LEPEWRDLRTVHDGMNHLFA a g c t c c g g t t c c c a a c g a t c a a g g c g a g t t a c a t g a t c c c c c a t g t t g t g c a a a a a a g c g t c g a g g c c a a g g g t t g c t a g t t c c g c t c a a t g t a c t a g g g g g t a c a a c a c g t t t t t t c g c

11,040 145 140 135 130 TLEKPGGITTLLLNAATNDS AmpR TLEKPGGITTLLLNAATNDS g t t a g c t c c t t c g g t c c t c c g a t c g t t g t c a g a a g t a a g t t g g c c g c a g t g t t a t c a c t c c a a t c g a g g a a g c c a g g a g g c t a g c a a c a g t c t t c a t t c a a c c g g c g t c a c a a t a g t g a g

11,100 125 120 115 110 MTIAASCLERVTMGDTLHKE AmpR MTIAASCLERVTMGDTLHKE a t g g t t a t g g c a g c a c t g c a t a a t t c t c t t a c t g t c a t g c c a t c c g t a a g a t g c t t t t c t t a c c a a t a c c g t c g t g a c g t a t t a a g a g a a t g a c a g t a c g g t a g g c a t t c t a c g a a a a g a

11,160 105 100 95 90 TVPSYEVLDNQSYHIRRGLQ AmpR TVPSYEVLDNQSYHIRRGLQ g t g a c t g g t g a g t a c t c a a c c a a g t c a t t c t g a g a a t a g t g t a t g c g g c g a c c g a g t t g c c a c t g a c c a c t c a t g a g t t g g t t c a g t a a g a c t c t t a t c a c a t a c g c c g c t g g c t c a a c g

11,220 85 80 75 70 EQGADIRSLVAGCLLVKFTS AmpR A T A A T A C C G C G C C A C A T A G C Amp-R EQGADIRSLVAGCLLVKFTS t c t t g c c c g g c g t c a a t a c g g g a t a a t a c c g c g c c a c a t a g c a g a a c t t t a a a a g t g c t c a g a a c g g g c c g c a g t t a t g c c c t a t t a t g g c g c g g t g t a t c g t c t t g a a a t t t t c a c g a g

11,280 65 60 55 50 MMPFREEPRFSELIKGSNLD AmpR MMPFREEPRFSELIKGSNLD a t c a t t g g a a a a c g t t c t t c g g g g c g a a a a c t c t c a a g g a t c t t a c c g c t g t t g a g a t c c t a g t a a c c t t t t g c a a g a a g c c c c g c t t t t g a g a g t t c c t a g a a t g g c g a c a a c t c t a g g

11,340 45 40 35 30 LEIYGVRAGLQDEADKVKVL AmpR LEIYGVRAGLQDEADKVKVL a g t t c g a t g t a a c c c a c t c g t g c a c c c a a c t g a t c t t c a g c a t c t t t t a c t t t c a c c a g c t c a a g c t a c a t t g g g t g a g c a c g t g g g t t g a c t a g a a g t c g t a g a a a a t g a a a g t g g t c g

11,400 25 TEPH 20 15 10 signal sequence AFVPLCFAAFFPILAV AmpR TEPHAFVPLCFAAFFPILAV g t t t c t g g g t g a g c a a a a a c a g g a a g g c a a a a t g c c g c a a a a a a g g g a a t a a g g g c g a c a c a a a g a c c c a c t c g t t t t t g t c c t t c c g t t t t a c g g c g t t t t t t c c c t t a t t c c c g c t g t

11,460 5 1 signal sequence RFHQISM AmpR AmpR promoter RFHQISM c g g a a a t g t t g a a t a c t c a t a c t c t t c c t t t t t c a a t a t t a t t g a a g c a t t t a t c a g g g t g c c t t t a c a a c t t a t g a g t a t g a g a a g g a a a a a g t t a t a a t a a c t t c g t a a a t a g t c c c a

11,520 AmpR promoter t a t t g t c t c a t g a g c g g a t a c a t a t t t g a a t g t a t t t a g a a a a a t a a a c a a a t a g g g g t t a t a a c a g a g t a c t c g c c t a t g t a t a a a c t t a c a t a a a t c t t t t t a t t t g t t t a t c c c c a a

11,580 AmpR promoter f1 ori c c g c g c a c a t t t c c c c g a a a a g t g c c a c c t a a a t t g t a a g c g t t a a t a t t t t g t t a a a a t g g c g c g t g t a a a g g g g c t t t t c a c g g t g g a t t t a a c a t t c g c a a t t a t a a a a c a a t t t t a

11,640 f1 ori t c g c g t t a a a t t t t t g t t a a a t c a g c t c a t t t t t t a a c c a a t a g g c c g a a a t c g g c a a a a a g c g c a a t t t a a a a a c a a t t t a g t c g a g t a a a a a a t t g g t t a t c c g g c t t t a g c c g t t t t

11,700 f1 ori G G T C A A A C C T T G T F1ori-F t c c c t t a t a a a t c a a a a g a a t a g a c c g a g a t a g g g t t g a g t g t t g t t c c a g t t t g g a a c a a g g g a a t a t t t a g t t t t c t t a t c t g g c t c t a t c c c a a c t c a c a a c a a g g t c a a a c c t t g t

11,760 f1 ori T C T C A G G T G F1ori-F a g a g t c c a c t a t t a a a g a a c g t g g a c t c c a a c g t c a a a g g g c g a a a a a c c g t c t a t c a g g t c t c a g g t g a t a a t t t c t t g c a c c t g a g g t t g c a g t t t c c c g c t t t t t g g c a g a t a g t c c

11,820 f1 ori g c g a t g g c c c a c t a c g t g a a c c a t c a c c c t a a t c a a g t t t t t t g g g g t c g a g g t g c c g t a c g c t a c c g g g t g a t g c a c t t g g t a g t g g g a t t a g t t c a a a a a a c c c c a g c t c c a c g g c a t

11,880 f1 ori a a g c a c t a a a t c g g a a c c c t a a a g g g a g c c c c c g a t t t a g a g c t t g a c g g g g a a a g c c g g t t c g t g a t t t a g c c t t g g g a t t t c c c t c g g g g g c t a a a t c t c g a a c t g c c c c t t t c g g c c

11,940 f1 ori A G G G A A G A A A G C G A A A G G A G F1ori-R c g a a c g t g g c g a g a a a g g a a g g g a a g a a a g c g a a a g g a g c g g g c g c t a g g g c g c t g g c a a g c t t g c a c c g c t c t t t c c t t c c c t t c t t t c g c t t t c c t c g c c c g c g a t c c c g c g a c c g t t

12,000 f1 ori g t g t a g c g g t c a c g c t g c g c g t a a c c a c c a c a c c c g c c g c g c t t a a t g c g c c g c t a c a g g c a c a t c g c c a g t g c g a c g c g c a t t g g t g g t g t g g g c g g c g c g a a t t a c g c g g c g a t g t c c

12,060 f1 ori g c g c g t c c c a t t c g c c a t t c a g g c t g c g c a a c t g t t g g g a a g g g c g a t c g g t g c g g g c c t c g c g c a g g g t a a g c g g t a a g t c c g a c g c g t t g a c a a c c c t t c c c g c t a g c c a c g c c c g g a

12,120 c t t c g c t a t t a c g c c a g c t g g c g a a a g g g g g a t g t g c t g c a a g g c g a t t a a g t t g g g t a a g a a g c g a t a a t g c g g t c g a c c g c t t t c c c c c t a c a c g a c g t t c c g c t a a t t c a a c c c a t t

12,180 M13 fwd T7 promoter C C C A G T C A C G A C G T T G T A A A A C G M13/pUC Forward T G T A A A A C G A C G G C C A G T M13 Forward T A A T A C G T7 c g c c a g g g t t t t c c c a g t c a c g a c g t t g t a a a a c g a c g g c c a g t g a g c g c g c g t a a t a c g g c g g t c c c a a a a g g g t c a g t g c t g c a a c a t t t t g c t g c c g g t c a c t c g c g c g c a t t a t g c

AvrII 12,240 T7 promoter SV40 ori A C T C A C T A T A G G G T7 a c t c a c t a t a g g g c g a a t t g g g t a c g t c c c t a g g c c t c c a a a a a a g c c t c c t c a c t a c t t t g a g t g a t a t c c c g c t t a a c c c a t g c a g g g a t c c g g a g g t t t t t t c g g a g g a g t g a t g a a

12,300 SV40 ori c t g g a a t a g c t c a g a g g c c g a g g c g g c c t c g g c c t c t g c a t a a a t a a a a a a a a t t a g t c a g a c c t t a t c g a g t c t c c g g c t c c g c c g g a g c c g g a g a c g t a t t t a t t t t t t t t a a t c a g t

12,360 SV40 ori g c c a t g g g g c g g a g a a t g g g c g g a a c t g g g c g g a g t t a g g g g c g g g a t g g g c g g a g t t a g c g g t a c c c c g c c t c t t a c c c g c c t t g a c c c g c c t c a a t c c c c g c c c t a c c c g c c t c a a t c

12,420 SV40 poly(A) signal C T A C T C A A A C C T G T T T G G T G EBV-rev g g g c g g g a t a g c t a g a g c c a g a c a t g a t a a g a t a c a t t g a t g a g t t t g g a c a a a c c a c a a c c c g c c c t a t c g a t c t c g g t c t g t a c t a t t c t a t g t a a c t a c t c a a a c c t g t t t g g t g t t

12,480 SV40 poly(A) signal G A A A T T T G T G A T G C T A T T G C SV40pA-R c t a g a a t g c a g t g a a a a a a a t g c t t t a t t t g t g a a a t t t g t g a t g c t a t t g c t t t a t t t g g a t c t t a c g t c a c t t t t t t t a c g a a a t a a a c a c t t t a a a c a c t a c g a t a a c g a a a t a a a c

12,540 SV40 poly(A) signal t a a c c a t t a t a a g c t g c a a t a a a c a a g t t c c t c t c a c t c t c t g a t a t t c a t t t c t t t g c a a t t g g t a a t a t t c g a c g t t a t t t g t t c a a g g a g a g t g a g a g a c t a t a a g t a a a g a a a c g t

12,600 3' LTR ( Δ U3) a g t t a t a a a t a c t g a a t a a t a a g a t g a c a t g a a c t a c t a c t g c t a g a g a t t t t c c a c a c t t c a a t a t t t a t g a c t t a t t a t t c t a c t g t a c t t g a t g a t g a c g a t c t c t a a a a g g t g t g a

12,660 3' LTR ( Δ U3) g a c t a a a a g g g t c t g a g g g a t c t c t a g t t a c c a g a g t c a c a c a a c a g a c g g g c a c a c a c t c t g a t t t t c c c a g a c t c c c t a g a g a t c a a t g g t c t c a g t g t g t t g t c t g c c c g t g t g t g a

12,720 3' LTR ( Δ U3) a c t t g a a g c a c t c a a g g c a a g c t t t a t t g a g g c t t a a g c a g t g g g t t c c c t a g t t a g c c a t g a a c t t c g t g a g t t c c g t t c g a a a t a a c t c c g a a t t c g t c a c c c a a g g g a t c a a t c g g t

12,780 3' LTR ( Δ U3) g a g a g c t c c c a g g c t c a g a t c t g g t c t a a c c a g a g a g a c c c a g t a c a a g c a a a a a g c a g a c t c t c g a g g g t c c g a g t c t a g a c c a g a t t g g t c t c t c t g g g t c a t g t t c g t t t t t c g t c t

BmtINheI 12,840 3' LTR ( Δ U3) t c t t g t c t t c g t t g g g a g t g a g c t a g c c c t t c c a g t c c c c c c t t t t c t t t t a a a a a g t g g a g a a c a g a a g c a a c c c t c a c t c g a t c g g g a a g g t c a g g g g g g a a a a g a a a a t t t t t c a c c

Acc65IKpnI 12,900 c t a a g a t c t a c a g c t g c c t t g t a a g t c a t t g g t c t t a a a g g t a c c g g g c c c t c g a c g g a t g a t t c t a g a t g t c g a c g g a a c a t t c a g t a a c c a g a a t t t c c a t g g c c c g g g a g c t g c c t a

12,960 265 260 255 250 *FFEDLLRYFAIRQSDPAA NeoR/KanR *FFEDLLRYFAIRQSDPAA c c c c t c a g a a g a a c t c g t c a a g a a g g c g a t a g a a g g c g a t g c g c t g c g a a t c g g g a g c g g g g g g a g t c t t c t t g a g c a g t t c t t c c g c t a t c t t c c g c t a c g c g a c g c t t a g c c c t c g c c

13,020 245 240 235 230 IGYLVLFRDAWEGGLEEAID NeoR/KanR T T A T A Neo-F IGYLVLFRDAWEGGLEEAID c g a t a c c g t a a a g c a c g a g g a a g c g g t c a g c c c a t t c g c c g c c a a g c t c t t c a g c a a t a t g c t a t g g c a t t t c g t g c t c c t t c g c c a g t c g g g t a a g c g g c g g t t c g a g a a g t c g t t a t a

RsrII 13,080 225 220 215 210 RTALAIDQYRDAVGLRGCDI NeoR/KanR G T G C C C A T C G G T T G C Neo-F RTALAIDQYRDAVGLRGCDI c a c g g g t a g c c a a c g c t a t g t c c t g a t a g c g g t c c g c c a c a c c c a g c c g g c c a c a g t c g a g t g c c c a t c g g t t g c g a t a c a g g a c t a t c g c c a g g c g g t g t g g g t c g g c c g g t g t c a g c t

13,140 205 200 195 190 FGSFRGNEVMINPLCADGHT NeoR/KanR FGSFRGNEVMINPLCADGHT t g a a t c c a g a a a a g c g g c c a t t t t c c a c c a t g a t a t t c g g c a a g c a g g c a t c g c c a t g g g a c t t a g g t c t t t t c g c c g g t a a a a g g t g g t a c t a t a a g c c g t t c g t c c g t a g c g g t a c c c

13,200 185 180 175 170 VVLDEGDPMRAKLRAFLEAP NeoR/KanR VVLDEGDPMRAKLRAFLEAP t c a c g a c g a g a t c c t c g c c g t c g g g c a t g c g c g c c t t g a g c c t g g c g a a c a g t t c g g c t g a g t g c t g c t c t a g g a g c g g c a g c c c g t a c g c g c g g a a c t c g g a c c g c t t g t c a a g c c g a c

13,260 165 160 155 150 ALGQHEEDLDDQDVLGAEMR NeoR/KanR ALGQHEEDLDDQDVLGAEMR g c g c g a g c c c c t g a t g c t c t t c g t c c a g a t c a t c c t g a t c g a c a a g a c c g g c t t c c a t c c c g c g c t c g g g g a c t a c g a g a a g c a g g t c t a g t a g g a c t a g c t g t t c t g g c c g a a g g t a g g

13,320 145 140 135 130 TRAREIRHKAQHDFPCTAPD NeoR/KanR TRAREIRHKAQHDFPCTAPD g a g t a c g t g c t c g c t c g a t g c g a t g t t t c g c t t g g t g g t c g a a t g g g c a g g t a g c c g g a t c t c a t g c a c g a g c g a g c t a c g c t a c a a a g c g a a c c a c c a g c t t a c c c g t c c a t c g g c c t a

13,380 125 120 115 110 LTHLRRMADAMISVKEAPAL NeoR/KanR LTHLRRMADAMISVKEAPAL c a a g c g t a t g c a g c c g c c g c a t t g c a t c a g c c a t g a t g g a t a c t t t c t c g g c a g g a g c a a g t t c g c a t a c g t c g g c g g c g t a a c g t a g t c g g t a c t a c c t a t g a a a g a g c c g t c c t c g t t

13,440 105 100 95 90 HSSLLDQGPVEGLLLWDRGA NeoR/KanR HSSLLDQGPVEGLLLWDRGA g g t g a g a t g a c a g g a g a t c c t g c c c c g g c a c t t c g c c c a a t a g c a g c c a g t c c c t t c c c g c c a c t c t a c t g t c c t c t a g g a c g g g g c c g t g a a g c g g g t t a t c g t c g g t c a g g g a a g g g c

13,500 85 80 75 70 ETVVDLVAACPVGTTALWSL NeoR/KanR ETVVDLVAACPVGTTALWSL c t t c a g t g a c a a c g t c g a g c a c a g c t g c g c a a g g a a c g c c c g t c g t g g c c a g c c a c g a t a g a a g t c a c t g t t g c a g c t c g t g t c g a c g c g t t c c t t g c g g g c a g c a c c g g t c g g t g c t a t

13,560 65 60 55 50 RAAEDQLENLAGSLDTKVFL NeoR/KanR RAAEDQLENLAGSLDTKVFL g c c g c g c t g c c t c g t c c t g c a g t t c a t t c a g g g c a c c g g a c a g g t c g g t c t t g a c a a a a a c g g c g c g a c g g a g c a g g a c g t c a a g t a a g t c c c g t g g c c t g t c c a g c c a g a a c t g t t t t t

13,620 45 40 35 30 VPRGQASLRFVAADSCGITQ NeoR/KanR VPRGQASLRFVAADSCGITQ g a a c c g g g c g c c c c t g c g c t g a c a g c c g g a a c a c g g c g g c a t c a g a g c a g c c g a t t g t c t c t t g g c c c g c g g g g a c g c g a c t g t c g g c c t t g t g c c g c c g t a g t c t c g t c g g c t a a c a g a

3ʹ 5ʹ 13,661 25 20 QAWDYGFLREVWA NeoR/KanR G C C C A G T C A T A G C C G A A T A G Neo-R QAWDYGFLREVWA g t t g t g c c c a g t c a t a g c c g a a t a g c c t c t c c a c c c a a g c g c a a c a c g g g t c a g t a t c g g c t t a t c g g a g a g g t g g g t t c g c

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.