Addgene MSCB-hDot1Lwt Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: MSCB-hDot1Lwt Sequencing Result


Map View

AfeI (9932) CMV-F (9307 .. 9327) CMV promoter SnaBI (9128) BtgZI (9122) NdeI (9022) AmpR promoter Amp-R (8350 .. 8369) ScaI (8280) PvuI (8170) FspI (8022) pBR322ori-F (7048 .. 7067) PciI (6907) L4440 (6797 .. 6814) CAP binding site lac operator EcoRV (6054) BmtI (5947) NheI (5943) BspDI - ClaI (5869) mPGK-F (5776 .. 5795) BspEI (5763) MSCV-rev (5379 .. 5402) PaeR7I - PspXI - XhoI (5327) NotI (5308) BsrGI (297) pLXSN 5' (347 .. 369) pBABE 5' (385 .. 401) EcoRI (419) HA PshAI (478) BsaBI (537) AleI (2469) PaqCI (2475) KflI (3387) MreI - SgrAI (4145) SrfI (4374) SfiI (4685) SalI (4703) HincII (4705) PmlI (4978) MSCB-hDot1Lwt 9994 bp

Sequence View

60 gag (truncated) g g c c a g a c t g t t a c c a c t c c c t t a a g t t t g a c c t t a g g t c a c t g g a a a g a t g t c g a g c g g c c g g t c t g a c a a t g g t g a g g g a a t t c a a a c t g g a a t c c a g t g a c c t t t c t a c a g c t c g c c

120 gag (truncated) a t c g c t c a c a a c c a g t c g g t a g a t g t c a a g a a g a g a c g t t g g g t t a c c t t c t g c t c t g c a t a g c g a g t g t t g g t c a g c c a t c t a c a g t t c t t c t c t g c a a c c c a a t g g a a g a c g a g a c g t

180 gag (truncated) g a a t g g c c a a c c t t t a a c g t c g g a t g g c c g c g a g a c g g c a c c t t t a a c c g a g a c c t c a t c c t t a c c g g t t g g a a a t t g c a g c c t a c c g g c g c t c t g c c g t g g a a a t t g g c t c t g g a g t a g

240 gag (truncated) a c c c a g g t t a a g a t c a a g g t c t t t t c a c c t g g c c c g c a t g g a c a c c c a g a c c a g g t c c c c t g g g t c c a a t t c t a g t t c c a g a a a a g t g g a c c g g g c g t a c c t g t g g g t c t g g t c c a g g g g

BsrGI 300 gag (truncated) t a c a t c g t g a c c t g g g a a g c c t t g g c t t t t g a c c c c c c t c c c t g g g t c a a g c c c t t t g t a a t g t a g c a c t g g a c c c t t c g g a a c c g a a a a c t g g g g g g a g g g a c c c a g t t c g g g a a a c a t

360 gag (truncated) C C C T T G A A C C T C C T pLXSN 5' c a c c c t a a g c c t c c g c c t c c t c t t c c t c c a t c c g c c c c g t c t c t c c c c c t t g a a c c t c c t g t g g g a t t c g g a g g c g g a g g a g a a g g a g g t a g g c g g g g c a g a g a g g g g g a a c t t g g a g g a

EcoRI 420 gag (truncated) C G T T C G A C C pLXSN 5' C T T T A T C C A G C C C T C A C pBABE 5' c g t t c g a c c c c g c c t c g a t c c t c c c t t t a t c c a g c c c t c a c t c c t t c t c t a g g c g c c g g a g c a a g c t g g g g c g g a g c t a g g a g g g a a a t a g g t c g g g a g t g a g g a a g a g a t c c g c g g c c t

PshAI 480 1 5 YPYDVPDYA (in frame with HA) GEKLELRLK HA MYPYDVPDYAGEKLELRLK a t t c a t g t a c c c a t a c g a c g t a c c a g a t t a c g c t g g g g a g a a g c t g g a g c t g a g a c t g a a t a a g t a c a t g g g t a t g c t g c a t g g t c t a a t g c g a c c c c t c t t c g a c c t c g a c t c t g a c t t

BsaBI 540 (in frame with HA) SPVGAEPAVYPWPLPVYDKH SPVGAEPAVYPWPLPVYDKH g t c g c c c g t g g g g g c t g a g c c c g c c g t c t a c c c g t g g c c g c t g c c g g t c t a c g a t a a a c a c a g c g g g c a c c c c c g a c t c g g g c g g c a g a t g g g c a c c g g c g a c g g c c a g a t g c t a t t t g t

600 (in frame with HA) HDAAHEIIETIRWVCEEIPD HDAAHEIIETIRWVCEEIPD t c a c g a t g c t g c t c a t g a a a t c a t c g a g a c c a t c c g a t g g g t c t g t g a a g a a a t c c c g g a a g t g c t a c g a c g a g t a c t t t a g t a g c t c t g g t a g g c t a c c c a g a c a c t t c t t t a g g g c c t

660 (in frame with HA) LKLAMENYVLIDYDTKSFES LKLAMENYVLIDYDTKSFES *NVIVGFAELA t c t c a a g c t c g c t a t g g a g a a t t a c g t t t t a a t t g a c t a t g a c a c c a a a a g c t t c g a g a g a g a g t t c g a g c g a t a c c t c t t a a t g c a a a a t t a a c t g a t a c t g t g g t t t t c g a a g c t c t c

720 (in frame with HA) MQRLCDKYNRAIDSIHQLWK MQRLCDKYNRAIDSIHQLWK HLPEAVLVVTGDVADVLQPL c a t g c a g a g g c t c t g c g a c a a g t a c a a c c g t g c c a t c g a c a g c a t c c a c c a g c t g t g g a a g t a c g t c t c c g a g a c g c t g t t c a t g t t g g c a c g g t a g c t g t c g t a g g t g g t c g a c a c c t t

780 (in frame with HA) GTTQPMKLNTRPSTGLLRHI GTTQPMKLNTRPSTGLLRHI AGRLGHLQVRPRGSSEQAMD g g g c a c c a c g c a g c c c a t g a a g c t g a a c a c g c g g c c g t c c a c t g g a c t c c t g c g c c a t a t c c c g t g g t g c g t c g g g t a c t t c g a c t t g t g c g c c g g c a g g t g a c c t g a g g a c g c g g t a t a

840 (in frame with HA) LQQVYNHSVTDPEKLNNYEP LQQVYNHSVTDPEKLNNYEP QLLDVVVRHGVGLLEVVVLG c c t g c a g c a g g t c t a c a a c c a c t c g g t g a c c g a c c c c g a g a a g c t c a a c a a c t a c g a g c c g g a c g t c g t c c a g a t g t t g g t g a g c c a c t g g c t g g g g c t c t t c g a g t t g t t g a t g c t c g g

900 (in frame with HA) FSPEVYGETSFDLVAQMIDE FSPEVYGETSFDLVAQMIDE EGGLHVPLGGEVQHGLHNIL c t t c t c c c c c g a g g t g t a c g g g g a g a c c t c c t t c g a c c t g g t g g c c c a g a t g a t t g a t g a g a a g a g g g g g c t c c a c a t g c c c c t c t g g a g g a a g c t g g a c c a c c g g g t c t a c t a a c t a c t

960 (in frame with HA) IKMTDDDLFVDLGSGVGQVV IKMTDDDLFVDLGSGVGQVV DLHGVVVQKHVQPATHALDH g a t c a a g a t g a c c g a c g a c g a c c t g t t t g t g g a c t t g g g g a g c g g t g t g g g c c a g g t c g t c t a g t t c t a c t g g c t g c t g c t g g a c a a a c a c c t g a a c c c c t c g c c a c a c c c g g t c c a g c a

1020 (in frame with HA) LQVAAATNCKHHYGVEKADI LQVAAATNCKHHYGVEKADI ELNSSGGVAFMVIADLFCVD g c t c c a g g t t g c t g c t g c c a c c a a c t g c a a a c a t c a c t a t g g c g t c g a g a a a g c a g a c a t c g a g g t c c a a c g a c g a c g g t g g t t g a c g t t t g t a g t g a t a c c g c a g c t c t t t c g t c t g t a

1080 (in frame with HA) PAKYAETMDREFRKWMKWYG PAKYAETMDREFRKWMKWYG RGLIRLGHVALEPLPHFPIS c c c g g c c a a g t a t g c g g a g a c c a t g g a c c g c g a g t t c a g g a a g t g g a t g a a a t g g t a t g g g g g c c g g t t c a t a c g c c t c t g g t a c c t g g c g c t c a a g t c c t t c a c c t a c t t t a c c a t a c c

1140 (in frame with HA) KKHAEYTLERGDFLSEEWRE KKHAEYTLERGDFLSEEWRE FLM a a a a a a g c a t g c a g a a t a c a c a t t g g a g a g a g g c g a t t t c c t c t c a g a a g a g t g g a g g g a t t t t t t c g t a c g t c t t a t g t g t a a c c t c t c t c c g c t a a a g g a g a g t c t t c t c a c c t c c c t

1200 (in frame with HA) RIANTSVIFVNNFAFGPEVD RIANTSVIFVNNFAFGPEVD g c g a a t c g c c a a c a c g a g t g t t a t a t t t g t g a a t a a t t t t g c c t t t g g t c c t g a g g t g g a c g c t t a g c g g t t g t g c t c a c a a t a t a a a c a c t t a t t a a a a c g g a a a c c a g g a c t c c a c c t

1260 (in frame with HA) HQLKERFANMKEGGRIVSSK HQLKERFANMKEGGRIVSSK t c a c c a g c t g a a g g a g c g g t t t g c a a a c a t g a a g g a a g g t g g c a g a a t c g t g t c c t c g a a a g t g g t c g a c t t c c t c g c c a a a c g t t t g t a c t t c c t t c c a c c g t c t t a g c a c a g g a g c t t

1320 (in frame with HA) PFAPLNFRINSRNLSDIGTI PFAPLNFRINSRNLSDIGTI a c c c t t t g c a c c t c t g a a c t t c a g a a t a a a c a g t a g a a a c t t g a g t g a c a t c g g c a c c a t t g g g a a a c g t g g a g a c t t g a a g t c t t a t t t g t c a t c t t t g a a c t c a c t g t a g c c g t g g t a

1380 (in frame with HA) MRVVELSPLKGSVSWTGKPV MRVVELSPLKGSVSWTGKPV c a t g c g c g t g g t g g a g c t c t c g c c c c t g a a g g g c t c g g t g t c g t g g a c g g g g a a g c c a g t g t a c g c g c a c c a c c t c g a g a g c g g g g a c t t c c c g a g c c a c a g c a c c t g c c c c t t c g g t c a

1440 (in frame with HA) SYYLHTIDRTILENYFSSLK SYYLHTIDRTILENYFSSLK c t c c t a c t a c c t g c a c a c t a t c g a c c g c a c c a t a c t t g a a a a c t a t t t t t c t a g t c t g a a g a g g a t g a t g g a c g t g t g a t a g c t g g c g t g g t a t g a a c t t t t g a t a a a a a g a t c a g a c t t

1500 (in frame with HA) NPKLREEQEAARRRQQRESK NPKLREEQEAARRRQQRESK a a a c c c a a a a c t c a g g g a g g a a c a g g a g g c a g c c c g g c g c c g c c a g c a g c g c g a g a g c a a t t t g g g t t t t g a g t c c c t c c t t g t c c t c c g t c g g g c c g c g g c g g t c g t c g c g c t c t c g t t

1560 (in frame with HA) SNAATPTKGPEGKVAGPADA SNAATPTKGPEGKVAGPADA g a g c a a c g c g g c c a c g c c c a c t a a g g g c c c a g a g g g c a a g g t g g c c g g c c c c g c c g a c g c c t c g t t g c g c c g g t g c g g g t g a t t c c c g g g t c t c c c g t t c c a c c g g c c g g g g c g g c t g c g

1620 (in frame with HA) PMDSGAEEEKAGAATVKKPS PMDSGAEEEKAGAATVKKPS c c c c a t g g a c t c t g g t g c t g a g g a a g a g a a g g c g g g a g c a g c c a c c g t g a a g a a g c c g t c g g g g t a c c t g a g a c c a c g a c t c c t t c t c t t c c g c c c t c g t c g g t g g c a c t t c t t c g g c a g

1680 (in frame with HA) PSKARKKKLNKKGRKMAGRK PSKARKKKLNKKGRKMAGRK t c c c t c c a a a g c c c g c a a g a a g a a g c t a a a c a a g a a g g g g a g g a a g a t g g c t g g c c g c a a a g g g a g g t t t c g g g c g t t c t t c t t c g a t t t g t t c t t c c c c t c c t t c t a c c g a c c g g c g t t

1740 (in frame with HA) RGRPKKMNTANPERKPKKNQ RGRPKKMNTANPERKPKKNQ g c g c g g g c g c c c c a a g a a g a t g a a c a c t g c g a a c c c c g a g c g g a a g c c c a a g a a g a a c c a c g c g c c c g c g g g g t t c t t c t a c t t g t g a c g c t t g g g g c t c g c c t t c g g g t t c t t c t t g g t

1800 (in frame with HA) TALDALHAQTVSQTAASSPQ TALDALHAQTVSQTAASSPQ a a c t g c a c t g g a t g c c c t g c a c g c t c a g a c c g t g t c t c a g a c g g c g g c c t c c t c a c c c c a t t g a c g t g a c c t a c g g g a c g t g c g a g t c t g g c a c a g a g t c t g c c g c c g g a g g a g t g g g g t

1860 (in frame with HA) DAYRSPHSPFYQLPPSVQRH DAYRSPHSPFYQLPPSVQRH g g a t g c c t a c a g a t c c c c t c a c a g c c c g t t c t a c c a g c t a c c t c c g a g c g t g c a g c g g c a c c t a c g g a t g t c t a g g g g a g t g t c g g g c a a g a t g g t c g a t g g a g g c t c g c a c g t c g c c g t

1920 (in frame with HA) SPNPLLVAPTPPALQKLLES SPNPLLVAPTPPALQKLLES c t c c c c c a a c c c g c t g c t g g t g g c g c c c a c c c c g c c c g c g c t g c a g a a g c t t c t a g a g t c g a g g g g g t t g g g c g a c g a c c a c c g c g g g t g g g g c g g g c g c g a c g t c t t c g a a g a t c t c a g

1980 (in frame with HA) FKIQYLQFLAYTKTPQYKAS FKIQYLQFLAYTKTPQYKAS c t t c a a g a t c c a g t a c c t g c a g t t c c t g g c a t a c a c a a a g a c c c c c c a g t a c a a g g c c a g g a a g t t c t a g g t c a t g g a c g t c a a g g a c c g t a t g t g t t t c t g g g g g g t c a t g t t c c g g t c

2040 (in frame with HA) LQELLGQEKEKNAQLLGAAQ LQELLGQEKEKNAQLLGAAQ c c t g c a g g a g c t g c t g g g c c a g g a g a a g g a g a a g a a c g c c c a g c t c c t g g g t g c g g c t c a g g a c g t c c t c g a c g a c c c g g t c c t c t t c c t c t t c t t g c g g g t c g a g g a c c c a c g c c g a g t

2100 (in frame with HA) QLLSHCQAQKEEIRRLFQQK QLLSHCQAQKEEIRRLFQQK g c a g c t c c t c a g c c a c t g c c a g g c c c a g a a g g a g g a g a t c a g g a g g c t g t t t c a g c a a a a c g t c g a g g a g t c g g t g a c g g t c c g g g t c t t c c t c c t c t a g t c c t c c g a c a a a g t c g t t t t

2160 (in frame with HA) LDELGVKALTYNDLIQAQKE LDELGVKALTYNDLIQAQKE a t t g g a t g a g c t g g g t g t g a a g g c g c t g a c c t a c a a c g a c c t g a t t c a a g c g c a g a a g g a t a a c c t a c t c g a c c c a c a c t t c c g c g a c t g g a t g t t g c t g g a c t a a g t t c g c g t c t t c c t

2220 (in frame with HA) ISAHNQQLREQSEQLEQDNR ISAHNQQLREQSEQLEQDNR g a t c t c c g c c c a t a a c c a g c a g c t g c g g g a g c a g t c g g a g c a g c t g g a g c a g g a c a a c c g c t a g a g g c g g g t a t t g g t c g t c g a c g c c c t c g t c a g c c t c g t c g a c c t c g t c c t g t t g g c

2280 (in frame with HA) ALRGQSLQLLKARCEELQLD ALRGQSLQLLKARCEELQLD c g c g c t c c g c g g c c a g a g c t t g c a g c t g c t c a a g g c t c g c t g c g a g g a g c t g c a g c t g g a g c g c g a g g c g c c g g t c t c g a a c g t c g a c g a g t t c c g a g c g a c g c t c c t c g a c g t c g a c c t

2340 (in frame with HA) WATLSLEKLLKEKQALKSQI WATLSLEKLLKEKQALKSQI c t g g g c c a c g c t g t c g c t g g a g a a g c t g t t g a a g g a g a a g c a g g c c c t g a a g a g c c a g a t g a c c c g g t g c g a c a g c g a c c t c t t c g a c a a c t t c c t c t t c g t c c g g g a c t t c t c g g t c t a

2400 (in frame with HA) SEKQRHCLELQISIVELEKS SEKQRHCLELQISIVELEKS c t c g g a g a a g c a g a g g c a c t g c c t g g a g c t g c a g a t c a g c a t t g t g g a g c t a g a g a a g a g g a g c c t c t t c g t c t c c g t g a c g g a c c t c g a c g t c t a g t c g t a a c a c c t c g a t c t c t t c t c

2460 (in frame with HA) QRQQELLQLKSCVPPDDALS QRQQELLQLKSCVPPDDALS c c a g c g g c a g c a g g a g c t c c t g c a g c t c a a g t c c t g t g t g c c g c c t g a c g a c g c c c t g t c g g t c g c c g t c g t c c t c g a g g a c g t c g a g t t c a g g a c a c a c g g c g g a c t g c t g c g g g a c a g

PaqCIAleI 2520 (in frame with HA) LHLRGKGALGRELEPDASRL LHLRGKGALGRELEPDASRL c c t g c a c c t g c g t g g g a a g g g c g c c c t g g g c c g c g a g c t g g a g c c t g a c g c c a g c c g g c t g g a c g t g g a c g c a c c c t t c c c g c g g g a c c c g g c g c t c g a c c t c g g a c t g c g g t c g g c c g a

2580 (in frame with HA) HLELDCTKFSLPHLSSMSPE HLELDCTKFSLPHLSSMSPE g c a c c t g g a g c t g g a c t g c a c c a a g t t c t c g c t g c c t c a c t t g a g c a g c a t g a g c c c g g a c g t g g a c c t c g a c c t g a c g t g g t t c a a g a g c g a c g g a g t g a a c t c g t c g t a c t c g g g c c t

2640 (in frame with HA) LSMNGQAAGYELCGVLSRPS LSMNGQAAGYELCGVLSRPS g c t c t c c a t g a a c g g c c a g g c t g c t g g c t a t g a g c t c t g c g g t g t g c t g a g c c g g c c t t c c g a g a g g t a c t t g c c g g t c c g a c g a c c g a t a c t c g a g a c g c c a c a c g a c t c g g c c g g a a g

2700 (in frame with HA) SKQNTPQYLASPLDQEVVPC SKQNTPQYLASPLDQEVVPC g t c g a a g c a g a a c a c g c c c c a g t a c c t g g c c t c a c c c c t g g a c c a g g a g g t g g t g c c c t g c a g c t t c g t c t t g t g c g g g g t c a t g g a c c g g a g t g g g g a c c t g g t c c t c c a c c a c g g g a c

2760 (in frame with HA) TPSHVGRPRLEKLSGLAAPD TPSHVGRPRLEKLSGLAAPD t a c c c c t a g c c a c g t c g g c c g g c c g c g c c t g g a g a a g c t g t c t g g c c t a g c c g c a c c c g a a t g g g g a t c g g t g c a g c c g g c c g g c g c g g a c c t c t t c g a c a g a c c g g a t c g g c g t g g g c t

2820 (in frame with HA) YTRLSPAKIVLRRHLSQDHT YTRLSPAKIVLRRHLSQDHT c t a c a c t a g g c t g t c c c c g g c c a a g a t t g t g c t g a g g c g g c a c c t g a g c c a g g a c c a c a c g a t g t g a t c c g a c a g g g g c c g g t t c t a a c a c g a c t c c g c c g t g g a c t c g g t c c t g g t g t g

2880 (in frame with HA) VPGRPAASELHSRAEHTKEN VPGRPAASELHSRAEHTKEN g g t g c c c g g c a g g c c g g c t g c c a g t g a g c t g c a t t c g a g a g c t g a g c a c a c c a a g g a g a a c c a c g g g c c g t c c g g c c g a c g g t c a c t c g a c g t a a g c t c t c g a c t c g t g t g g t t c c t c t t

2940 (in frame with HA) GLPYQSPSVPGSMKLSPQDP GLPYQSPSVPGSMKLSPQDP c g g c c t t c c c t a c c a g a g c c c c a g c g t g c c t g g c a g c a t g a a g c t g a g c c c t c a g g a c c c g c c g g a a g g g a t g g t c t c g g g g t c g c a c g g a c c g t c g t a c t t c g a c t c g g g a g t c c t g g g

3000 (in frame with HA) RPLSPGALQLAGEKSSEKGL RPLSPGALQLAGEKSSEKGL g c g g c c c c t g t c c c c t g g g g c c t t g c a g c t t g c t g g a g a g a a g a g c a g t g a g a a g g g c c t c g c c g g g g a c a g g g g a c c c c g g a a c g t c g a a c g a c c t c t c t t c t c g t c a c t c t t c c c g g a

3060 (in frame with HA) RERAYGSSGELITSLPISIP RERAYGSSGELITSLPISIP g a g a g a g c g c g c c t a c g g c a g c a g c g g g g a g c t c a t c a c c a g c c t g c c c a t c a g c a t c c c c t c t c t c g c g c g g a t g c c g t c g t c g c c c c t c g a g t a g t g g t c g g a c g g g t a g t c g t a g g g

3120 (in frame with HA) LSTVQPNKLPVSIPLASVVL LSTVQPNKLPVSIPLASVVL g c t c a g c a c c g t g c a g c c c a a c a a g c t c c c g g t c a g c a t t c c c c t g g c c a g c g t g g t g c t c g a g t c g t g g c a c g t c g g g t t g t t c g a g g g c c a g t c g t a a g g g g a c c g g t c g c a c c a c g a

3180 (in frame with HA) PSRAERARSTPSPVLQPRDP PSRAERARSTPSPVLQPRDP g c c c a g c c g c g c c g a g a g g g c g a g g a g c a c c c c c a g t c c c g t g c t g c a g c c c c g t g a c c c c g g g t c g g c g c g g c t c t c c c g c t c c t c g t g g g g g t c a g g g c a c g a c g t c g g g g c a c t g g g

3240 (in frame with HA) SSTLEKQIGANAHGAGSRSL SSTLEKQIGANAHGAGSRSL c t c g t c c a c a c t t g a a a a g c a g a t t g g t g c t a a t g c c c a c g g t g c t g g g a g c a g a a g c c t g a g c a g g t g t g a a c t t t t c g t c t a a c c a c g a t t a c g g g t g c c a c g a c c c t c g t c t t c g g a

3300 (in frame with HA) ALAPAGFSYAGSVAISGALA ALAPAGFSYAGSVAISGALA *APETAMLPAKA t g c c c t g g c c c c c g c a g g c t t c t c c t a c g c t g g c t c g g t g g c c a t c a g c g g g g c c t t g g c a c g g g a c c g g g g g c g t c c g a a g a g g a t g c g a c c g a g c c a c c g g t a g t c g c c c c g g a a c c g

3360 (in frame with HA) GSPASLTPGAEPATLDESSS GSPASLTPGAEPATLDESSS PLGAERVGPASGAVKSSDEL g g g c a g c c c g g c c t c t c t c a c a c c t g g a g c c g a g c c g g c c a c c t t g g a t g a g t c c t c c a g c c c g t c g g g c c g g a g a g a g t g t g g a c c t c g g c t c g g c c g g t g g a a c c t a c t c a g g a g g t c

KflI 3420 (in frame with HA) SGSLFATVGSRSSTPQHPLL SGSLFATVGSRSSTPQHPLL EPLRKAVTPDRLEVGCCGRS c t c t g g g a g c c t t t t t g c c a c c g t g g g g t c c c g c a g c t c c a c g c c a c a g c a c c c c c t g c t g a g a c c c t c g g a a a a a c g g t g g c a c c c c a g g g c g t c g a g g t g c g g t g t c g t g g g g g a c g a

3480 (in frame with HA) LAQPRNSLPASPAHQLSSSP LAQPRNSLPASPAHQLSSSP SACGRFESGAEGAWWSEELG g c t g g c a c a g c c c c g g a a c t c g c t t c c t g c c t c t c c c g c c c a c c a g c t c t c c t c c a g t c c c g a c c g t g t c g g g g c c t t g a g c g a a g g a c g g a g a g g g c g g g t g g t c g a g a g g a g g t c a g g

3540 (in frame with HA) RLGGAAQGPLPEASKGDLPS RLGGAAQGPLPEASKGDLPS RSPPAAWPGNGSALLPSRGE c c g g c t t g g t g g g g c c g c c c a g g g c c c g t t g c c c g a g g c c a g c a a g g g a g a c c t g c c c t c g g c c g a a c c a c c c c g g c g g g t c c c g g g c a a c g g g c t c c g g t c g t t c c c t c t g g a c g g g a g

3600 (in frame with HA) DSGFSDPESEAKRRIVFTIT DSGFSDPESEAKRRIVFTIT SEPKESGSLSALLLITNVMV c g a t t c c g g c t t c t c a g a t c c t g a g a g t g a a g c c a a g a g g a g g a t t g t g t t c a c c a t c a c g c t a a g g c c g a a g a g t c t a g g a c t c t c a c t t c g g t t c t c c t c c t a a c a c a a g t g g t a g t g

3660 (in frame with HA) TGAGSAKQSPSSKHSPLTAS TGAGSAKQSPSSKHSPLTAS VPAPLALCDGELLCLGRVAL c a c t g g t g c g g g c a g t g c c a a g c a g t c g c c c t c c a g c a a g c a c a g c c c c c t g a c c g c c a g g t g a c c a c g c c c g t c a c g g t t c g t c a g c g g g a g g t c g t t c g t g t c g g g g g a c t g g c g g t c

3720 (in frame with HA) ARGDCVPSHGQDSRRRGRRK ARGDCVPSHGQDSRRRGRRK ARPSQTGLWPCSLRLRPRRF c g c c c g t g g g g a c t g t g t g c c g a g c c a c g g g c a g g a c a g t c g c a g g c g c g g c c g g c g g a a g c g g g c a c c c c t g a c a c a c g g c t c g g t g c c c g t c c t g t c a g c g t c c g c g c c g g c c g c c t t

3780 (in frame with HA) RASAGTPSLSAGVSPKRRAL RASAGTPSLSAGVSPKRRAL RADAPVGLKLAPTDGLRRAR g c g a g c a t c t g c g g g g a c g c c c a g c t t g a g c g c a g g c g t g t c c c c c a a g c g c c g a g c c c t c g c t c g t a g a c g c c c c t g c g g g t c g a a c t c g c g t c c g c a c a g g g g g t t c g c g g c t c g g g a

3840 (in frame with HA) PSVAGLFTQPSGSPLNLNSM PSVAGLFTQPSGSPLNLNSM GDTAPRKVCGEPDGRLRLEM g c c g t c c g t c g c t g g c c t t t t c a c a c a g c c t t c g g g g t c t c c c c t c a a c c t c a a c t c c a t c g g c a g g c a g c g a c c g g a a a a g t g t g t c g g a a g c c c c a g a g g g g a g t t g g a g t t g a g g t a

3900 (in frame with HA) VSNINQPLEITAISSPETSL VSNINQPLEITAISSPETSL TLLMLWGRSIVAM g g t c a g t a a c a t c a a c c a g c c c c t g g a g a t t a c a g c c a t c t c g t c c c c g g a g a c c t c c c t c c a g t c a t t g t a g t t g g t c g g g g a c c t c t a a t g t c g g t a g a g c a g g g g c c t c t g g a g g g a

3960 (in frame with HA) KSSPVPYQDHDQPPVLKKER KSSPVPYQDHDQPPVLKKER g a a g a g c t c c c c t g t g c c c t a c c a g g a c c a c g a c c a g c c c c c c g t g c t c a a g a a g g a g c g c t t c t c g a g g g g a c a c g g g a t g g t c c t g g t g c t g g t c g g g g g g c a c g a g t t c t t c c t c g c

4020 (in frame with HA) PLSQTNGAHYSPLTSDEEPG PLSQTNGAHYSPLTSDEEPG g c c t c t g a g c c a g a c c a a t g g g g c a c a c t a c t c c c c a c t c a c c t c a g a c g a g g a g c c a g g c g g a g a c t c g g t c t g g t t a c c c c g t g t g a t g a g g g g t g a g t g g a g t c t g c t c c t c g g t c c

4080 (in frame with HA) SEDEPSSARIERKIATISLE SEDEPSSARIERKIATISLE c t c t g a g g a c g a g c c c a g c a g t g c t c g a a t t g a g a g a a a a a t t g c a a c a a t c t c c t t a g a g a g a c t c c t g c t c g g g t c g t c a c g a g c t t a a c t c t c t t t t t a a c g t t g t t a g a g g a a t c t

4140 (in frame with HA) SKSPPKTLENGGGLAGRKPA SKSPPKTLENGGGLAGRKPA a a g c a a a t c t c c c c c g a a a a c c t t g g a a a a t g g t g g t g g c t t g g c g g g a a g g a a g c c c g c t t c g t t t a g a g g g g g c t t t t g g a a c c t t t t a c c a c c a c c g a a c c g c c c t t c c t t c g g g c g

SgrAIMreI 4200 (in frame with HA) PAGEPVNSSKWKSTFSPISD PAGEPVNSSKWKSTFSPISD g c c c g c c g g c g a g c c a g t c a a t a g c a g c a a g t g g a a g t c c a c c t t c t c g c c c a t c t c c g a c g g g c g g c c g c t c g g t c a g t t a t c g t c g t t c a c c t t c a g g t g g a a g a g c g g g t a g a g g c t

4260 (in frame with HA) IGLAKSADSPLQASSALSQN IGLAKSADSPLQASSALSQN c a t c g g c c t g g c c a a g t c g g c g g a c a g c c c g c t g c a g g c c a g c t c c g c c c t c a g c c a g a a g t a g c c g g a c c g g t t c a g c c g c c t g t c g g g c g a c g t c c g g t c g a g g c g g g a g t c g g t c t t

4320 (in frame with HA) SLFTFRPALEEPSADAKLAA SLFTFRPALEEPSADAKLAA c t c c c t g t t c a c g t t c c g g c c c g c c c t g g a g g a g c c c t c t g c c g a t g c c a a g c t g g c c g c g a g g g a c a a g t g c a a g g c c g g g c g g g a c c t c c t c g g g a g a c g g c t a c g g t t c g a c c g g c g

SrfI 4380 (in frame with HA) HPRKGFPGSLSGADGLSPGT HPRKGFPGSLSGADGLSPGT *GLFPKGPERDPASPSLGPV t c a c c c c a g g a a a g g c t t t c c c g g c t c c c t g t c g g g g g c t g a c g g a c t c a g c c c g g g c a c a g t g g g g t c c t t t c c g a a a g g g c c g a g g g a c a g c c c c c g a c t g c c t g a g t c g g g c c c g t g

4440 (in frame with HA) NPANGCTFGGGLAADLSLHS NPANGCTFGGGLAADLSLHS LGALPQVKPPPRAASRLKCL c a a c c c t g c c a a c g g c t g c a c c t t c g g c g g g g g c c t g g c c g c g g a c c t g a g t t t a c a c a g g t t g g g a c g g t t g c c g a c g t g g a a g c c g c c c c c g g a c c g g c g c c t g g a c t c a a a t g t g t c

4500 (in frame with HA) FSDGASLPHKGPEAAGLSSP FSDGASLPHKGPEAAGLSSP KLSPAERGWLPGSAAPRLEG c t t c a g t g a t g g t g c t t c t c t t c c c c a c a a g g g c c c c g a g g c g g c c g g c c t g a g c t c c c c g a a g t c a c t a c c a c g a a g a g a a g g g g t g t t c c c g g g g c t c c g c c g g c c g g a c t c g a g g g g

4560 (in frame with HA) LSFPSQRGKEGSDANPFLSK LSFPSQRGKEGSDANPFLSK SLKGECRPLSPESALGKRLL g c t g a g c t t c c c c t c g c a g c g c g g c a a g g a g g g c t c g g a c g c c a a c c c t t t c c t g a g c a a c g a c t c g a a g g g g a g c g t c g c g c c g t t c c t c c c g a g c c t g c g g t t g g g a a a g g a c t c g t t

4620 (in frame with HA) RQLDGLAGLKGEGSRGKEAG RQLDGLAGLKGEGSRGKEAG LCSSPRAPSFPSPLRPLSAP g a g g c a g c t g g a c g g c c t g g c t g g g c t g a a g g g c g a g g g c a g c c g c g g c a a g g a g g c a g g c t c c g t c g a c c t g c c g g a c c g a c c c g a c t t c c c g c t c c c g t c g g c g c c g t t c c t c c g t c c

4680 (in frame with HA) EGGLPLCGPTDKTPLLSGKA EGGLPLCGPTDKTPLLSGKA SPPRGSHPGVSLVGSSLPLA *RQAPGRVLGWQQAALG g g a g g g c g g c c t a c c g c t g t g c g g g c c c a c g g a c a a g a c c c c a c t g c t g a g c g g c a a g g c c c t c c c g c c g g a t g g c g a c a c g c c c g g g t g c c t g t t c t g g g g t g a c g a c t c g c c g t t c c g

SalIHincIISfiI 4740 (in frame with HA) AKARDREVDLKNGHNLFISA AKARDREVDLKNGHNLFISA ALARSRSTSRLFPWLRKMEA GLGPVALDVELIAVVEEDRR c g c c a a g g c c c g g g a c c g c g a g g t c g a c c t c a a g a a t g g c c a c a a c c t c t t c a t c t c t g c g c g g t t c c g g g c c c t g g c g c t c c a g c t g g a g t t c t t a c c g g t g t t g g a g a a g t a g a g a c g

4800 (in frame with HA) AAVPPGSLLSGPGLAPAASS AAVPPGSLLSGPGLAPAASS AATGGPLRRLPGPRAGAADE RGHRGSAEEAAGAQGRRRGG g g c g g c c g t g c c t c c c g g a a g c c t c c t c a g c g g c c c c g g c c t g g c c c c g g c g g c g t c c t c c c g c c g g c a c g g a g g g c c t t c g g a g g a g t c g c c g g g g c c g g a c c g g g g c c g c c g c a g g a g

4860 (in frame with HA) AGGAASSAQTHRSFLGPFPP AGGAASSAQTHRSFLGPFPP APPAADEAWVCRDKRPGKGG CAARRGGGLRVPGEQAGERR c g c a g g c g g c g c g g c g t c c t c c g c c c a g a c g c a c c g g t c c t t c c t g g g c c c c t t c c c g c c g c g t c c g c c g c g c c g c a g g a g g c g g g t c t g c g t g g c c a g g a a g g a c c c g g g g a a g g g c g g

4920 (in frame with HA) GPQFALGPMSLQANLGSVAG GPQFALGPMSLQANLGSVAG PGCNASPGMDRCALRPETAP SRLEREAGHGQLGVEAGHGA g g g a c c g c a g t t c g c g c t c g g c c c c a t g t c c c t g c a g g c c a a c c t c g g c t c c g t g g c c g g c c c t g g c g t c a a g c g c g a g c c g g g g t a c a g g g a c g t c c g g t t g g a g c c g a g g c a c c g g c c

PmlI 4980 (in frame with HA) SSVLQSLFSSVPAAAGLVHV SSVLQSLFSSVPAAAGLVHV EETSCDSNLETGAAAPRTCT GGHQLRQEARHRGGCAQHVH c t c c t c c g t g c t g c a g t c g c t g t t c a g c t c t g t g c c g g c c g c c g c a g g c c t g g t g c a c g t g a g g a g g c a c g a c g t c a g c g a c a a g t c g a g a c a c g g c c g g c g g c g t c c g g a c c a c g t g c a

5040 (in frame with HA) SSAATRLTNSHAMGSFSGVA SSAATRLTNSHAMGSFSGVA DDAAVLSVLECAM RGSGGSQGVRVGHAAKGPHC g t c g t c c g c t g c c a c c a g a c t g a c c a a c t c g c a c g c c a t g g g c a g c t t t t c c g g g g t g g c c a g c a g g c g a c g g t g g t c t g a c t g g t t g a g c g t g c g g t a c c c g t c g a a a a g g c c c c a c c g

5100 (in frame with HA) GGTVGGN* GGTVGGN* AACNSTVLIEVEVALGICPR a g g c g g c a c a g t t g g a g g t a a c t a g g a t t t c t a c c t c a a c c g c g a g a c c t a t g c a a g g a c t c c g c c g t g t c a a c c t c c a t t g a t c c t a a a g a t g g a g t t g g c g c t c t g g a t a c g t t c c t g

5160 HPGVRARPM g g t g t g g a c c a a c t c g c g c c c g c g g c a t g g t g c c c g c c g g c c t g c c g g g c t c c c a c c c c t c c a c a c c t g g t t g a g c g c g g g c g c c g t a c c a c g g g c g g c c g g a c g g c c c g a g g g t g g g g a

5220 g g a c g g c a g a g g c a a g g a c g g a c g g g a g c t c c a c t g t g a a t c g g c g g c a c g c g c c g c a g g c c t g c c g t c t c c g t t c c t g c c t g c c c t c g a g g t g a c a c t t a g c c g c c g t g c g c g g c g t c c

5280 a g g c t g g g a c t g g t c c a g t t t g t a c t g t c g a t a g t t t t a g a t a a a g t a t t t a t c a t t t t t t c c g a c c c t g a c c a g g t c a a a c a t g a c a g c t a t c a a a a t c t a t t t c a t a a a t a g t a a a a a

PaeR7IPspXIXhoINotI 5340 t a a a a a g t a a a a a a a a a a a a a a a a g g g c g g c c g c t c t a g a g t a t c c c t c g a g t t a a t t c t a t t t t t c a t t t t t t t t t t t t t t t t c c c g c c g g c g a g a t c t c a t a g g g a g c t c a a t t a a g a

5400 PGK promoter C G T A C G C G A A A T C G T C G G G G C G MSCV-rev a c c g g g t a g g g g a g g c g c t t t t c c c a a g g c a g t c t g g a g c a t g c g c t t t a g c a g c c c c g c t g g c c c a t c c c c t c c g c g a a a a g g g t t c c g t c a g a c c t c g t a c g c g a a a t c g t c g g g g c g

5460 PGK promoter A C MSCV-rev t g g g c a c t t g g c g c t a c a c a a g t g g c c t c t g g c c t c g c a c a c a t t c c a c a t c c a c c g g t a a c c c g t g a a c c g c g a t g t g t t c a c c g g a g a c c g g a g c g t g t g t a a g g t g t a g g t g g c c a t

5520 PGK promoter g g c g c c a a c c g g c t c c g t t c t t t g g t g g c c c c t t c g c g c c a c c t t c t a c t c c t c c c c t a g c c g c g g t t g g c c g a g g c a a g a a a c c a c c g g g g a a g c g c g g t g g a a g a t g a g g a g g g g a t c

5580 PGK promoter t c a g g a a g t t c c c c c c c g c c c c g c a g c t c g c g t c g t g c a g g a c g t g a c a a a t g g a a g t a g a g t c c t t c a a g g g g g g g c g g g g c g t c g a g c g c a g c a c g t c c t g c a c t g t t t a c c t t c a t c

5640 PGK promoter c a c g t c t c a c t a g t c t c g t g c a g a t g g a c a g c a c c g c t g a g c a a t g g a a g c g g g t a g g c c g t g c a g a g t g a t c a g a g c a c g t c t a c c t g t c g t g g c g a c t c g t t a c c t t c g c c c a t c c g g

5700 PGK promoter t t t g g g g c a g c g g c c a a t a g c a g c t t t g c t c c t t c g c t t t c t g g g c t c a g a g g c t g g g a a a a a c c c c g t c g c c g g t t a t c g t c g a a a c g a g g a a g c g a a a g a c c c g a g t c t c c g a c c c t t

5760 PGK promoter g g g g t g g g t c c g g g g g c g g g c t c a g g g g c g g g c t c a g g g g c g g g g c g g g c g c c c g a a g g t c c c c a c c c a g g c c c c c g c c c g a g t c c c c g c c c g a g t c c c c g c c c c g c c c g c g g g c t t c c a

BspEI 5820 PGK promoter C A T T C T G C A C G C T T C A A A A G mPGK-F c c t c c g g a g g c c c g g c a t t c t g c a c g c t t c a a a a g c g c a c g t c t g c c g c g c t g t t c t c c t g g a g g c c t c c g g g c c g t a a g a c g t g c g a a g t t t t c g c g t g c a g a c g g c g c g a c a a g a g g a

BspDIClaI 5880 PGK promoter c t t c c t c a t c t c c g g g c c t t t c g g g a t c t c g a c c t g c a g c c a a g c t t a t c g a t a a a a t a a g a a g g a g t a g a g g c c c g g a a a g c c c t a g a g c t g g a c g t c g g t t c g a a t a g c t a t t t t a t t

5940 3' LTR a a g a t t t t a t t t a g t c t c c a g a a a a a g g g g g g a a t g a a a g a c c c c a c c t g t a g g t t t g g c t t c t a a a a t a a a t c a g a g g t c t t t t t c c c c c c t t a c t t t c t g g g g t g g a c a t c c a a a c c g

BmtINheI 6000 3' LTR a a g c t a g c t t a a g t a a c g c c a t t t t g c a a g g c a t g g a a a a t a c a t a a c t g a g a a t a g a g a t t c g a t c g a a t t c a t t g c g g t a a a a c g t t c c g t a c c t t t t a t g t a t t g a c t c t t a t c t c t

EcoRV 6060 3' LTR a g t t c a g a t c a a g g t t a g g a a c a g a g a g a c a g c a g a a t a t g g g c c a a a c a g g a t a t c t g t t c a a g t c t a g t t c c a a t c c t t g t c t c t c t g t c g t c t t a t a c c c g g t t t g t c c t a t a g a c a

6120 3' LTR g g t a a g c a g t t c c t g c c c c g g c t c a g g g c c a a g a a c a g a t g g t c c c c a g a t g c g g t c c c g c c a t t c g t c a a g g a c g g g g c c g a g t c c c g g t t c t t g t c t a c c a g g g g t c t a c g c c a g g g c

6180 3' LTR c c c t c a g c a g t t t c t a g a g a a c c a t c a g a t g t t t c c a g g g t g c c c c a a g g a c c t g a a a t g g g g a g t c g t c a a a g a t c t c t t g g t a g t c t a c a a a g g t c c c a c g g g g t t c c t g g a c t t t a c

6240 3' LTR a c c c t g t g c c t t a t t t g a a c t a a c c a a t c a g t t c g c t t c t c g c t t c t g t t c g c g c g c t t c t g g g a c a c g g a a t a a a c t t g a t t g g t t a g t c a a g c g a a g a g c g a a g a c a a g c g c g c g a a g

6300 3' LTR t g c t c c c c g a g c t c a a t a a a a g a g c c c a c a a c c c c t c a c t c g g c g c g c c a g t c c t c c g a t a c g a g g g g c t c g a g t t a t t t t c t c g g g t g t t g g g g a g t g a g c c g c g c g g t c a g g a g g c t a

6360 3' LTR a g a c t g c g t c g c c c g g g t a c c c g t g t a t c c a a t a a a c c c t c t t g c a g t t g c a t c c g a c t t t c t g a c g c a g c g g g c c c a t g g g c a c a t a g g t t a t t t g g g a g a a c g t c a a c g t a g g c t g a a

6420 3' LTR g t g g t c t c g c t g t t c c t t g g g a g g g t c t c c t c t g a g t g a t t g a c t a c c c g t c a g c g g g g g c a c c a g a g c g a c a a g g a a c c c t c c c a g a g g a g a c t c a c t a a c t g a t g g g c a g t c g c c c c c

6480 3' LTR t c t t t c a t g g g t a a c a g t t t c t t g a a g t t g g a g a a c a a c a t t c t g a g g g t a g g a g t c g a a a g a a a g t a c c c a t t g t c a a a g a a c t t c a a c c t c t t g t t g t a a g a c t c c c a t c c t c a g c t t

6540 t a t t a a g t a a t c c t g a c t c a a t t a g c c a c t g t t t t g a a t c c a c a t a c t c c a a t a c t c c t g a t a a t t c a t t a g g a c t g a g t t a a t c g g t g a c a a a a c t t a g g t g t a t g a g g t t a t g a g g a c

6600 lac operator a a a t a g t t c a t t a t g g a c a g c g c a g a a a g a g c t g g g g a g a a t t g t g a a a t t g t t a t c c g c t t t a t c a a g t a a t a c c t g t c g c g t c t t t c t c g a c c c c t c t t a a c a c t t t a a c a a t a g g c g

6660 -10 -35 lac operator lac promoter CAP binding site t c a c a a t t c c a c a c a a c a t a c g a g c c g g a a g c a t a a a g t g t a a a g c c t g g g g t g c c t a a t a g t g t t a a g g t g t g t t g t a t g c t c g g c c t t c g t a t t t c a c a t t t c g g a c c c c a c g g a t t a

6720 CAP binding site g a g t g a g c t a a c t c a c a t t a a t t g c g t t g c g c t c a c t g c c c g c t t t c c a g t c g g g a a a c c c t c a c t c g a t t g a g t g t a a t t a a c g c a a c g c g a g t g a c g g g c g a a a g g t c a g c c c t t t g g

6780 t g t c g t g c c a g c t g c a t t a a t g a a t c g g c c a a c g c g c g g g g a g a g g c g g t t t g c g t a t t g a c a g c a c g g t c g a c g t a a t t a c t t a g c c g g t t g c g c g c c c c t c t c c g c c a a a c g c a t a a c

6840 G A G C G A G T G A C T G A G C G A L4440 g g c g c t c t t c c g c t t c c t c g c t c a c t g a c t c g c t g c g c t c g g t c g t t c g g c t g c g g c g a g c c g c g a g a a g g c g a a g g a g c g a g t g a c t g a g c g a c g c g a g c c a g c a a g c c g a c g c c g c t c

6900 c g g t a t c a g c t c a c t c a a a g g c g g t a a t a c g g t t a t c c a c a g a a t c a g g g g a t a a c g c a g g c c a t a g t c g a g t g a g t t t c c g c c a t t a t g c c a a t a g g t g t c t t a g t c c c c t a t t g c g t c

PciI 6960 g a a a g a a c a t g t g a g c a a a a g g c c a g c a a a a g g c c a g g a a c c g t a a a a a g g c c g c g t t g c c t t t c t t g t a c a c t c g t t t t c c g g t c g t t t t c c g g t c c t t g g c a t t t t t c c g g c g c a a c g

7020 ori t g g c g t t t t t c c a t a g g c t c c g c c c c c c t g a c g a g c a t c a c a a a a a t c g a c g c t c a a g t c a c c g c a a a a a g g t a t c c g a g g c g g g g g g a c t g c t c g t a g t g t t t t t a g c t g c g a g t t c a g

7080 ori T T T C T A T G G T C C G C A A A G G G pBR322ori-F a g a g g t g g c g a a a c c c g a c a g g a c t a t a a a g a t a c c a g g c g t t t c c c c c t g g a a g c t c c c t c t c c a c c g c t t t g g g c t g t c c t g a t a t t t c t a t g g t c c g c a a a g g g g g a c c t t c g a g g g

7140 ori t c g t g c g c t c t c c t g t t c c g a c c c t g c c g c t t a c c g g a t a c c t g t c c g c c t t t c t c c c t t a g c a c g c g a g a g g a c a a g g c t g g g a c g g c g a a t g g c c t a t g g a c a g g c g g a a a g a g g g a a

7200 ori c g g g a a g c g t g g c g c t t t c t c a t a g c t c a c g c t g t a g g t a t c t c a g t t c g g t g t a g g t c g g c c c t t c g c a c c g c g a a a g a g t a t c g a g t g c g a c a t c c a t a g a g t c a a g c c a c a t c c a g c

7260 ori t t c g c t c c a a g c t g g g c t g t g t g c a c g a a c c c c c c g t t c a g c c c g a c c g c t g c g c c t t a t a a g c g a g g t t c g a c c c g a c a c a c g t g c t t g g g g g g c a a g t c g g g c t g g c g a c g c g g a a t a

7320 ori c c g g t a a c t a t c g t c t t g a g t c c a a c c c g g t a a g a c a c g a c t t a t c g c c a c t g g c a g c a g g g c c a t t g a t a g c a g a a c t c a g g t t g g g c c a t t c t g t g c t g a a t a g c g g t g a c c g t c g t c

7380 ori c c a c t g g t a a c a g g a t t a g c a g a g c g a g g t a t g t a g g c g g t g c t a c a g a g t t c t t g a a g t g g t g a c c a t t g t c c t a a t c g t c t c g c t c c a t a c a t c c g c c a c g a t g t c t c a a g a a c t t c a

7440 ori g g t g g c c t a a c t a c g g c t a c a c t a g a a g a a c a g t a t t t g g t a t c t g c g c t c t g c t g a a g c c c a c c g g a t t g a t g c c g a t g t g a t c t t c t t g t c a t a a a c c a t a g a c g c g a g a c g a c t t c g

7500 ori c a g t t a c c t t c g g a a a a a g a g t t g g t a g c t c t t g a t c c g g c a a a c a a a c c a c c g c t g g t a g t c a a t g g a a g c c t t t t t c t c a a c c a t c g a g a a c t a g g c c g t t t g t t t g g t g g c g a c c a t

7560 ori g c g g t g g t t t t t t t g t t t g c a a g c a g c a g a t t a c g c g c a g a a a a a a a g g a t c t c a a g a a g c g c c a c c a a a a a a a c a a a c g t t c g t c g t c t a a t g c g c g t c t t t t t t t c c t a g a g t t c t t c

7620 a t c c t t t g a t c t t t t c t a c g g g g t c t g a c g c t c a g t g g a a c g a a a a c t c a c g t t a a g g g a t a g g a a a c t a g a a a a g a t g c c c c a g a c t g c g a g t c a c c t t g c t t t t g a g t g c a a t t c c c t

7680 t t t t g g t c a t g a g a t t a t c a a a a a g g a t c t t c a c c t a g a t c c t t t t a a a t t a a a a a t g a a a a a a c c a g t a c t c t a a t a g t t t t t c c t a g a a g t g g a t c t a g g a a a a t t t a a t t t t t a c t t

7740 285 *WHKI AmpR *WHKI g t t t t a a a t c a a t c t a a a g t a t a t a t g a g t a a a c t t g g t c t g a c a g t t a c c a a t g c t t a a c a a a a t t t a g t t a g a t t t c a t a t a t a c t c a t t t g a a c c a g a c t g t c a a t g g t t a c g a a t t

7800 280 275 270 265 LSAGIEAIQRNREDMTAQSG AmpR LSAGIEAIQRNREDMTAQSG t c a g t g a g g c a c c t a t c t c a g c g a t c t g t c t a t t t c g t t c a t c c a t a g t t g c c t g a c t c c a g t c a c t c c g t g g a t a g a g t c g c t a g a c a g a t a a a g c a a g t a g g t a t c a a c g g a c t g a g g

7860 260 255 250 245 TTYIVVIRSPKGDPGLAAII AmpR TTYIVVIRSPKGDPGLAAII c c g t c g t g t a g a t a a c t a c g a t a c g g g a g g g c t t a c c a t c t g g c c c c a g t g c t g c a a t g a g g c a g c a c a t c t a t t g a t g c t a t g c c c t c c c g a a t g g t a g a c c g g g g t c a c g a c g t t a c t

7920 240 235 230 225 GRSGREGAGSKDAIFWGAPL AmpR GRSGREGAGSKDAIFWGAPL t a c c g c g a g a c c c a c g c t c a c c g g c t c c a g a t t t a t c a g c a a t a a a c c a g c c a g c c g g a a a t g g c g c t c t g g g t g c g a g t g g c c g a g g t c t a a a t a g t c g t t a t t t g g t c g g t c g g c c t t

7980 220 215 210 205 ASRLLPGAVKDAEMWDILQQ AmpR ASRLLPGAVKDAEMWDILQQ g g g c c g a g c g c a g a a g t g g t c c t g c a a c t t t a t c c g c c t c c a t c c a g t c t a t t a a t t g t t c c c g g c t c g c g t c t t c a c c a g g a c g t t g a a a t a g g c g g a g g t a g g t c a g a t a a t t a a c a a

FspI 8040 200 195 190 185 RSALTLLEGTLLKRLTTAMA AmpR RSALTLLEGTLLKRLTTAMA g c c g g g a a g c t a g a g t a a g t a g t t c g c c a g t t a a t a g t t t g c g c a a c g t t g t t g c c a t t g c g g c c c t t c g a t c t c a t t c a t c a a g c g g t c a a t t a t c a a a c g c g t t g c a a c a a c g g t a a c

8100 180 175 170 165 VPMTTDREDNPIAENLEPEW AmpR VPMTTDREDNPIAENLEPEW c t a c a g g c a t c g t g g t g t c a c g c t c g t c g t t t g g t a t g g c t t c a t t c a g c t c c g g t t c c c g a t g t c c g t a g c a c c a c a g t g c g a g c a g c a a a c c a t a c c g a a g t a a g t c g a g g c c a a g g g

8160 160 155 150 145 RDLRTVHDGMNHLFATLEKP AmpR RDLRTVHDGMNHLFATLEKP a a c g a t c a a g g c g a g t t a c a t g a t c c c c c a t g t t g t g c a a a a a a g c g g t t a g c t c c t t c g t t g c t a g t t c c g c t c a a t g t a c t a g g g g g t a c a a c a c g t t t t t t c g c c a a t c g a g g a a g c

PvuI 8220 140 135 130 125 GGITTLLLNAATNDSMTIAA AmpR GGITTLLLNAATNDSMTIAA g t c c t c c g a t c g t t g t c a g a a g t a a g t t g g c c g c a g t g t t a t c a c t c a t g g t t a t g g c a g c a g g a g g c t a g c a a c a g t c t t c a t t c a a c c g g c g t c a c a a t a g t g a g t a c c a a t a c c g t c

ScaI 8280 120 115 110 105 SCLERVTMGDTLHKETVPSY AmpR SCLERVTMGDTLHKETVPSY c a c t g c a t a a t t c t c t t a c t g t c a t g c c a t c c g t a a g a t g c t t t t c t g t g a c t g g t g a g t g t g a c g t a t t a a g a g a a t g a c a g t a c g g t a g g c a t t c t a c g a a a a g a c a c t g a c c a c t c a

8340 100 95 90 85 EVLDNQSYHIRRGLQEQGAD AmpR EVLDNQSYHIRRGLQEQGAD a c t c a a c c a a g t c a t t c t g a g a a t a g t g t a t g c g g c g a c c g a g t t g c t c t t g c c c g g c g t t g a g t t g g t t c a g t a a g a c t c t t a t c a c a t a c g c c g c t g g c t c a a c g a g a a c g g g c c g c a

8400 80 75 70 65 IRSLVAGCLLVKFTSMMPFR AmpR A T A A T A C C G C G C C A C A T A G C Amp-R IRSLVAGCLLVKFTSMMPFR c a a t a c g g g a t a a t a c c g c g c c a c a t a g c a g a a c t t t a a a a g t g c t c a t c a t t g g a a a a c g t t a t g c c c t a t t a t g g c g c g g t g t a t c g t c t t g a a a t t t t c a c g a g t a g t a a c c t t t t g

8460 60 55 50 45 EEPRFSELIKGSNLDLEIYG AmpR EEPRFSELIKGSNLDLEIYG g t t c t t c g g g g c g a a a a c t c t c a a g g a t c t t a c c g c t g t t g a g a t c c a g t t c g a t g t a a c c a a g a a g c c c c g c t t t t g a g a g t t c c t a g a a t g g c g a c a a c t c t a g g t c a a g c t a c a t t g

8520 40 35 30 25 VRAGLQDEADKVKVLTEPH A AmpR VRAGLQDEADKVKVLTEPHA c c a c t c g t g c a c c c a a c t g a t c t t c a g c a t c t t t t a c t t t c a c c a g c g t t t c t g g g t g a g g g t g a g c a c g t g g g t t g a c t a g a a g t c g t a g a a a a t g a a a g t g g t c g c a a a g a c c c a c t c

8580 20 15 10 5 signal sequence FVPLCFAAFFPILAVRFHQI AmpR FVPLCFAAFFPILAVRFHQI c a a a a a c a g g a a g g c a a a a t g c c g c a a a a a a g g g a a t a a g g g c g a c a c g g a a a t g t t g a a g t t t t t g t c c t t c c g t t t t a c g g c g t t t t t t c c c t t a t t c c c g c t g t g c c t t t a c a a c t t

8640 1 signal sequ... SM AmpR AmpR promoter SM t a c t c a t a c t c t t c c t t t t t c a a t a t t a t t g a a g c a t t t a t c a g g g t t a t t g t c t c a t g a a t g a g t a t g a g a a g g a a a a a g t t a t a a t a a c t t c g t a a a t a g t c c c a a t a a c a g a g t a c t

8700 AmpR promoter g c g g a t a c a t a t t t g a a t g t a t t t a g a a a a a t a a a c a a a t a g g g g t t c c g c g c a c a t t t c c g c c t a t g t a t a a a c t t a c a t a a a t c t t t t t a t t t g t t t a t c c c c a a g g c g c g t g t a a a g

8760 c c c g a a a a g t g c c a c c a g c t t t g c t c t t a g g a g t t t c c t a a t a c a t c c c a a a c t c a a a t a g g g c t t t t c a c g g t g g t c g a a a c g a g a a t c c t c a a a g g a t t a t g t a g g g t t t g a g t t t a t

8820 t a t a a a g c a t t t g a c t t g t t c t a t g c c c t a g t t a t t a a t a g t a a t c a a t t a c g g g g t c a t a t a t t t c g t a a a c t g a a c a a g a t a c g g g a t c a a t a a t t a t c a t t a g t t a a t g c c c c a g t a

8880 CMV enhancer t a g t t c a t a g c c c a t a t a t g g a g t t c c g c g t t a c a t a a c t t a c g g t a a a t g g c c c g c c t g a t c a a g t a t c g g g t a t a t a c c t c a a g g c g c a a t g t a t t g a a t g c c a t t t a c c g g g c g g a c

8940 CMV enhancer g c t g a c c g c c c a a c g a c c c c c g c c c a t t g a c g t c a a t a a t g a c g t a t g t t c c c a t a g t a a c g a c t g g c g g g t t g c t g g g g g c g g g t a a c t g c a g t t a t t a c t g c a t a c a a g g g t a t c a t t

9000 CMV enhancer c g c c a a t a g g g a c t t t c c a t t g a c g t c a a t g g g t g g a g t a t t t a c g g t a a a c t g c c c a c t g c g g t t a t c c c t g a a a g g t a a c t g c a g t t a c c c a c c t c a t a a a t g c c a t t t g a c g g g t g a

NdeI 9060 CMV enhancer t g g c a g t a c a t c a a g t g t a t c a t a t g c c a a g t a c g c c c c c t a t t g a c g t c a a t g a c g g t a a c c g t c a t g t a g t t c a c a t a g t a t a c g g t t c a t g c g g g g g a t a a c t g c a g t t a c t g c c a t

9120 CMV enhancer a a t g g c c c g c c t g g c a t t a t g c c c a g t a c a t g a c c t t a t g g g a c t t t c c t a c t t g g c a g t t t a c c g g g c g g a c c g t a a t a c g g g t c a t g t a c t g g a a t a c c c t g a a a g g a t g a a c c g t c a

SnaBIBtgZI 9180 CMV enhancer CMV promoter a c a t c t a c g t a t t a g t c a t c g c t a t t a c c a t g g t g a t g c g g t t t t g g c a g t a c a t c a a t g t g t a g a t g c a t a a t c a g t a g c g a t a a t g g t a c c a c t a c g c c a a a a c c g t c a t g t a g t t a c

9240 CMV promoter g g c g t g g a t a g c g g t t t g a c t c a c g g g g a t t t c c a a g t c t c c a c c c c a t t g a c g t c a a t g c c g c a c c t a t c g c c a a a c t g a g t g c c c c t a a a g g t t c a g a g g t g g g g t a a c t g c a g t t a c

9300 CMV promoter g g a g t t t g t t t t g g c a c c a a a a t c a a c g g g a c t t t c c a a a a t g t c g t a a c a a c t c c g c c c c c t c a a a c a a a a c c g t g g t t t t a g t t g c c c t g a a a g g t t t t a c a g c a t t g t t g a g g c g g g

9360 CMV promoter C G C A A A T G G G C G G T A G G C G T G CMV-F c a t t g a c g c a a a t g g g c g g t a g g c g t g t a c g g t g g g a g g t c t a t a t a a g c a g a g c t c a a t g t a a c t g c g t t t a c c c g c c a t c c g c a c a t g c c a c c c t c c a g a t a t a t t c g t c t c g a g t t a

9420 a a a a g a g c c c a c a a c c c c t c a c t c g g c g c g c c a g t c c t c c g a t t g a c t g a g t c g c c c g g g t t t t c t c g g g t g t t g g g g a g t g a g c c g c g c g g t c a g g a g g c t a a c t g a c t c a g c g g g c c c

9480 t a c c c g t g t a t c c a a t a a a c c c t c t t g c a g t t g c a t c c g a c t t g t g g t c t c g c t g t t c c t a t g g g c a c a t a g g t t a t t t g g g a g a a c g t c a a c g t a g g c t g a a c a c c a g a g c g a c a a g g a

9540 t g g g a g g g t c t c c t c t g a g t g a t t g a c t a c c c g t c a g c g g g g g t c t t t c a t t t g g g g g c t a c c c t c c c a g a g g a g a c t c a c t a a c t g a t g g g c a g t c g c c c c c a g a a a g t a a a c c c c c g a

9600 MMLV Ψ c g t c c g g g a t c g g g a g a c c c c t g c c c a g g g a c c a c c g a c c c a c c a c c g g g a g g t a a g c t g g c a g g c c c t a g c c c t c t g g g g a c g g g t c c c t g g t g g c t g g g t g g t g g c c c t c c a t t c g a c

9660 MMLV Ψ g c c a g c a a c t t a t c t g t g t c t g t c c g a t t g t c t a g t g t c t a t g a c t g a t t t t a t g c g c c t c g g t c g t t g a a t a g a c a c a g a c a g g c t a a c a g a t c a c a g a t a c t g a c t a a a a t a c g c g g a

9720 MMLV Ψ g c g t c g g t a c t a g t t a g c t a a c t a g c t c t g t a t c t g g c g g a c c c g t g g t g g a a c t g a c g a c g c a g c c a t g a t c a a t c g a t t g a t c g a g a c a t a g a c c g c c t g g g c a c c a c c t t g a c t g c t

9780 MMLV Ψ g t t c t g a a c a c c c g g c c g c a a c c c t g g g a g a c g t c c c a g g g a c t t t g g g g g c c g t t t t t g c a a g a c t t g t g g g c c g g c g t t g g g a c c c t c t g c a g g g t c c c t g a a a c c c c c g g c a a a a a c

9840 MMLV Ψ t g g c c c g a c c t g a g g a a g g g a g t c g a t g t g g a a t c c g a c c c c g t c a g g a t a t g t g g t t c t a c c g g g c t g g a c t c c t t c c c t c a g c t a c a c c t t a g g c t g g g g c a g t c c t a t a c a c c a a g a

9900 g g t a g g a g a c g a g a a c c t a a a a c a g t t c c c g c c t c c g t c t g a a t t t t t g c t t t c g g t t t g c c a t c c t c t g c t c t t g g a t t t t g t c a a g g g c g g a g g c a g a c t t a a a a a c g a a a g c c a a a c

AfeI 9960 g a a c c g a a g c c g c g c g t c t t g t c t g c t g c a g c g c t g c a g c a t c g t t c t g t g t t g t c t c t g c t t g g c t t c g g c g c g c a g a a c a g a c g a c g t c g c g a c g t c g t a g c a a g a c a c a a c a g a g a c

9994 t c t g a c t g t g t t t c t g t a t t t g t c t g a a a a t t a g a g a c t g a c a c a a a g a c a t a a a c a g a c t t t t a a t c

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.