Addgene Rab24 cds in pGem-T Easy Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: Rab24 cds in pGem-T Easy Sequencing Result


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AhdI (2973) BsaI (2907) BpmI (2904) NmeAIII (2826) ScaI (2492) Amp-R (2404 .. 2423) XmnI (2373) PsiI (1906) F1ori-F (1845 .. 1866) pBR322ori-F (56 .. 75) PciI (211) L4440 (309 .. 326) BspQI - SapI (328) CAP binding site M13/pUC Reverse (522 .. 544) M13 Reverse (541 .. 557) M13 rev SP6 (575 .. 592) NsiI (609) MluI (614) NdeI (633) SalI (638) HincII (640) PstI - SbfI (648) BfuAI - BspMI (651) SpeI (664) AvaI - BsoBI (720) BsmBI - Esp3I (834) RsrII (902) EcoNI (912) Acc65I (927) KpnI (931) PflMI * (987) BstAPI (1153) EcoO109I - PpuMI (1180) BsrGI (1201) Bpu10I (1260) SacII (1320) NcoI - StyI (1326) SphI (1345) PspOMI (1353) ApaI (1357) T7 (1365 .. 1384) M13 fwd M13 Forward (1391 .. 1408) M13/pUC Forward (1400 .. 1422) F1ori-R (1635 .. 1654) NgoMIV (1673) NaeI (1675) BtgZI (1773) BsaAI (1778) DraIII (1781) Rab24 cds in pGem-T Easy 3650 bp

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60 ori G G G A A pBR322ori-F g g t a t c c g g t a a g c g g c a g g g t c g g a a c a g g a g a g c g c a c g a g g g a g c t t c c a g g g g g a a c c a t a g g c c a t t c g c c g t c c c a g c c t t g t c c t c t c g c g t g c t c c c t c g a a g g t c c c c c t t

120 ori A C G C C T G G T A T C T T T pBR322ori-F a c g c c t g g t a t c t t t a t a g t c c t g t c g g g t t t c g c c a c c t c t g a c t t g a g c g t c g a t t t t t g c g g a c c a t a g a a a t a t c a g g a c a g c c c a a a g c g g t g g a g a c t g a a c t c g c a g c t a a a a

180 ori t g t g a t g c t c g t c a g g g g g g c g g a g c c t a t g g a a a a a c g c c a g c a a c g c g g c c t t t t t a c a c a c t a c g a g c a g t c c c c c c g c c t c g g a t a c c t t t t t g c g g t c g t t g c g c c g g a a a a a t g

PciI 240 g g t t c c t g g c c t t t t g c t g g c c t t t t g c t c a c a t g t t c t t t c c t g c g t t a t c c c c t g a t t c c a a g g a c c g g a a a a c g a c c g g a a a a c g a g t g t a c a a g a a a g g a c g c a a t a g g g g a c t a a

300 c t g t g g a t a a c c g t a t t a c c g c c t t t g a g t g a g c t g a t a c c g c t c g c c g c a g c c g a a c g a g a c a c c t a t t g g c a t a a t g g c g g a a a c t c a c t c g a c t a t g g c g a g c g g c g t c g g c t t g c t

BspQISapI 360 A G C G A G T C A G T G A G C G A G L4440 c c g a g c g c a g c g a g t c a g t g a g c g a g g a a g c g g a a g a g c g c c c a a t a c g c a a a c c g c c t c g g c t c g c g t c g c t c a g t c a c t c g c t c c t t c g c c t t c t c g c g g g t t a t g c g t t t g g c g g a g

420 t c c c c g c g c g t t g g c c g a t t c a t t a a t g c a g c t g g c a c g a c a g g t t t c c c g a c t g g a a a g a g g g g c g c g c a a c c g g c t a a g t a a t t a c g t c g a c c g t g c t g t c c a a a g g g c t g a c c t t t c

480 CAP binding site lac promoter c g g g c a g t g a g c g c a a c g c a a t t a a t g t g a g t t a g c t c a c t c a t t a g g c a c c c c a g g c t t g c c c g t c a c t c g c g t t g c g t t a a t t a c a c t c a a t c g a g t g a g t a a t c c g t g g g g t c c g a a

540 -35 -10 lac promoter lac operator A G C G G A T A A C A A T T T C A C A M13/pUC Reverse t a c a c t t t a t g c t t c c g g c t c g t a t g t t g t g t g g a a t t g t g a g c g g a t a a c a a t t t c a c a a t g t g a a a t a c g a a g g c c g a g c a t a c a a c a c a c c t t a a c a c t c g c c t a t t g t t a a a g t g t

600 M13 rev SP6 promoter C A G G M13/pUC Reverse C A G G A A A C A G C T A T G A C M13 Reverse A T T T A G G T G A C A C T A T A G SP6 c a g g a a a c a g c t a t g a c c a t g a t t a c g c c a a g c t a t t t a g g t g a c a c t a t a g a a t a c t c a g t c c t t t g t c g a t a c t g g t a c t a a t g c g g t t c g a t a a a t c c a c t g t g a t a t c t t a t g a g t

SbfIPstIBspMIBfuAINdeISalIHincIIMluINsiI 660 a g c t a t g c a t c c a a c g c g t t g g g a g c t c t c c c a t a t g g t c g a c c t g c a g g c g g c c g c g a a t c g a t a c g t a g g t t g c g c a a c c c t c g a g a g g g t a t a c c a g c t g g a c g t c c g c c g g c g c t t

SpeI 720 *NHCCNDFYSDKKQ t t c a c t a g t g a t t c a g g t c a g t t a t g g c a g c a g t t g t c a a a a t a a g a g t c c t t c t t t t g c a a g t g a t c a c t a a g t c c a g t c a a t a c c g t c g t c a a c a g t t t t a t t c t c a g g a a g a a a a c g

BsoBIAvaI 780 GLDVGKEEQMFELACSSFDE c c g a g a t c c a c c c c c t t c t c c t c t t g c a t g a a c t c t a a a g c a c a g c t a c t a a a a t c t t c a g g c t c t a g g t g g g g g a a g a g g a g a a c g t a c t t g a g a t t t c g t g t c g a t g a t t t t a g a a g t

BsmBIEsp3I 840 AVKQFVEDVNKGTKSSTEFH g c c a c c t t c t g a a a c a c c t c a t c a a c a t t t t t t c c t g t t t t g c t g g a c g t c t c g a a a t g t c g g t g g a a g a c t t t g t g g a g t a g t t g t a a a a a a g g a c a a a a c g a c c t g c a g a g c t t t a c a

900 QAGIEDAFDQVDHYDVQRTS t g t g c a c c t a t t t c a t c t g c a a a g t c t t g a a c a t c a t g g t a g t c t a c t t g c c g c g t g c t t a c a c g t g g a t a a a g t a g a c g t t t c a g a a c t t g t a g t a c c a t c a g a t g a a c g g c g c a c g a a

Acc65IKpnIEcoNIRsrII 960 RDAEILDSKTGCLYIKCHEE c g g t c c g c c t c a a t c a g g t c a c t t t t g g t a c c a c a t a g a t a t a t t t t g c a g t g c t c c t c a g c c a g g c g g a g t t a g t c c a g t g a a a a c c a t g g t g t a t c t a t a t a a a a c g t c a c g a g g a g t

PflMI* 1020 CNQLEKVWFRARGFSSSDTL c a g t t c t g c a a c t c c t t c a c c c a g a a c c t g g c c c t t c c a a a a c t g c t g c t g t c a g t c a a g g t c a a g a c g t t g a g g a a g t g g g t c t t g g a c c g g g a a g g t t t t g a c g a c g a c a g t c a g t t c

1080 DYCVIAARAGRYYIRSMAEY t c a t a g c a g a c a a t g g c a g c a c g a g c t c c t c t g t a g t a a a t t c t g c t c a t t g c t t c a t a a a g t a t c g t c t g t t a c c g t c g t g c t c g a g g a g a c a t c a t t t a a g a c g a g t a a c g a a g t a t t

1140 RESGATDWIGLTIVKEGVNL c g t t c a g a c c c a g c g g t g t c c c a t a t t c c c a g t g t a a t c a c c t t c t c a c c a a c a t t g a g g g c a a g t c t g g g t c g c c a c a g g g t a t a a g g g t c a c a t t a g t g g a a g a g t g g t t g t a a c t c c

EcoO109IPpuMIBstAPI 1200 AKAVFAAGITNQYPGVLFRR g c t t t t g c a a c a a a t g c a g c c c c t a t a g t a t t t t g g t a a g g t c c c a c a a g a a a g c g a c g a c g a a a a c g t t g t t t a c g t c g g g g a t a t c a t a a a a c c a t t c c a g g g t g t t c t t t c g c t g c t

BsrGI 1260 HVYREVLSTKGVSEKGLMVV t g t a c a t a t c t c t c c a c c a g a c t g g t t t t a c c a a c g c t t t c t t t g c c a a g c a t g a c a a c c a c a t g t a t a g a g a g g t g g t c t g a c c a a a a t g g t t g c g a a a g a a a c g g t t c g t a c t g t t g g

SacIIBpu10I 1320 KADVRMATM t t a g c g t c c a c t c g c a t g g c a g t c a t g a t g a t a t g g g t t t g t g c a a a a t c g a a t t c c c g c a a t c g c a g g t g a g c g t a c c g t c a g t a c t a c t a t a c c c a a a c a c g t t t t a g c t t a a g g g c g

SphIPspOMIApaINcoIStyI 1380 Kozak sequence T7 promoter Kozak sequence G G G A T A T C A C T C A G C A T7 g g c c g c c a t g g c g g c c g g g a g c a t g c g a c g t c g g g c c c a a t t c g c c c t a t a g t g a g t c g t c c g g c g g t a c c g c c g g c c c t c g t a c g c t g c a g c c c g g g t t a a g c g g g a t a t c a c t c a g c a

1440 T7 promoter M13 fwd T A A T T7 T G A C C G G C A G C A A A A T G T M13 Forward G C A A A A T G T T G C A G C A C T G A C C C M13/pUC Forward a t t a c a a t t c a c t g g c c g t c g t t t t a c a a c g t c g t g a c t g g g a a a a c c c t g g c g t t a c c c t a a t g t t a a g t g a c c g g c a g c a a a a t g t t g c a g c a c t g a c c c t t t t g g g a c c g c a a t g g g

1500 a a c t t a a t c g c c t t g c a g c a c a t c c c c c t t t c g c c a g c t g g c g t a a t a g c g a a g a g g c c c t t g a a t t a g c g g a a c g t c g t g t a g g g g g a a a g c g g t c g a c c g c a t t a t c g c t t c t c c g g g

1560 f1 ori g c a c c g a t c g c c c t t c c c a a c a g t t g c g c a g c c t g a a t g g c g a a t g g a c g c g c c c t g t a g c g t g g c t a g c g g g a a g g g t t g t c a a c g c g t c g g a c t t a c c g c t t a c c t g c g c g g g a c a t c

1620 f1 ori c g g c g c a t t a a g c g c g g c g g g t g t g g t g g t t a c g c g c a g c g t g a c c g c t a c a c t t g c c a g g c c g c g t a a t t c g c g c c g c c c a c a c c a c c a a t g c g c g t c g c a c t g g c g a t g t g a a c g g t c

NgoMIVNaeI 1680 f1 ori G A G G A A A G C G A A A G A A G G G A F1ori-R c g c c c t a g c g c c c g c t c c t t t c g c t t t c t t c c c t t c c t t t c t c g c c a c g t t c g c c g g c t t g c g g g a t c g c g g g c g a g g a a a g c g a a a g a a g g g a a g g a a a g a g c g g t g c a a g c g g c c g a a

1740 f1 ori t c c c c g t c a a g c t c t a a a t c g g g g g c t c c c t t t a g g g t t c c g a t t t a g t g c t t t a c g g c a a g g g g c a g t t c g a g a t t t a g c c c c c g a g g g a a a t c c c a a g g c t a a a t c a c g a a a t g c c g t

BtgZIBsaAIDraIII 1800 f1 ori c c t c g a c c c c a a a a a a c t t g a t t a g g g t g a t g g t t c a c g t a g t g g g c c a t c g c c c t g a t a g g a g c t g g g g t t t t t t g a a c t a a t c c c a c t a c c a a g t g c a t c a c c c g g t a g c g g g a c t a t

1860 f1 ori G T G G A C T C T T G T T C C A F1ori-F g a c g g t t t t t c g c c c t t t g a c g t t g g a g t c c a c g t t c t t t a a t a g t g g a c t c t t g t t c c a c t g c c a a a a a g c g g g a a a c t g c a a c c t c a g g t g c a a g a a a t t a t c a c c t g a g a a c a a g g t

PsiI 1920 f1 ori A A C T G G F1ori-F a a c t g g a a c a a c a c t c a a c c c t a t c t c g g t c t a t t c t t t t g a t t t a t a a g g g a t t t t g c c t t g a c c t t g t t g t g a g t t g g g a t a g a g c c a g a t a a g a a a a c t a a a t a t t c c c t a a a a c g g

1980 f1 ori g a t t t c g g c c t a t t g g t t a a a a a a t g a g c t g a t t t a a c a a a a a t t t a a c g c g a a t t t t a a c t a a a g c c g g a t a a c c a a t t t t t t a c t c g a c t a a a t t g t t t t t a a a t t g c g c t t a a a a t t

2040 f1 ori c a a a a t a t t a a c g c t t a c a a t t t c c t g a t g c g g t a t t t t c t c c t t a c g c a t c t g t g c g g t g t t t t a t a a t t g c g a a t g t t a a a g g a c t a c g c c a t a a a a g a g g a a t g c g t a g a c a c g c c a

2100 AmpR promoter a t t t c a c a c c g c a t c a g g t g g c a c t t t t c g g g g a a a t g t g c g c g g a a c c c c t a t t t g t t t t a a a g t g t g g c g t a g t c c a c c g t g a a a a g c c c c t t t a c a c g c g c c t t g g g g a t a a a c a a a

2160 AmpR promoter a t t t t t c t a a a t a c a t t c a a a t a t g t a t c c g c t c a t g a g a c a a t a a c c c t g a t a a a t g c t t a a a a a g a t t t a t g t a a g t t t a t a c a t a g g c g a g t a c t c t g t t a t t g g g a c t a t t t a c g a

2220 1 5 10 signal sequence MSIQHFRVALIP AmpR promoter AmpR MSIQHFRVALIP t c a a t a a t a t t g a a a a a g g a a g a g t a t g a g t a t t c a a c a t t t c c g t g t c g c c c t t a t t c c a g t t a t t a t a a c t t t t t c c t t c t c a t a c t c a t a a g t t g t a a a g g c a c a g c g g g a a t a a g g

2280 15 20 signal sequence FFAAFCLPVFA 25 30 HPETLVKVK AmpR FFAAFCLPVFAHPETLVKVK c t t t t t t g c g g c a t t t t g c c t t c c t g t t t t t g c t c a c c c a g a a a c g c t g g t g a a a g t a a a g a a a a a a c g c c g t a a a a c g g a a g g a c a a a a a c g a g t g g g t c t t t g c g a c c a c t t t c a t t t

2340 35 40 45 50 DAEDQLGARVGYIELDLNSG AmpR DAEDQLGARVGYIELDLNSG a g a t g c t g a a g a t c a g t t g g g t g c a c g a g t g g g t t a c a t c g a a c t g g a t c t c a a c a g c g g t c t a c g a c t t c t a g t c a a c c c a c g t g c t c a c c c a a t g t a g c t t g a c c t a g a g t t g t c g c c

XmnI 2400 55 60 65 70 KILESFRPEERFPMMSTFKV AmpR KILESFRPEERFPMMSTFKV t a a g a t c c t t g a g a g t t t t c g c c c c g a a g a a c g t t t t c c a a t g a t g a g c a c t t t t a a a g t a t t c t a g g a a c t c t c a a a a g c g g g g c t t c t t g c a a a a g g t t a c t a c t c g t g a a a a t t t c a

2460 75 80 85 90 LLCGAVLSRIDAGQEQLGRR AmpR C G A T A C A C C G C G C C A T A A T A Amp-R LLCGAVLSRIDAGQEQLGRR t c t g c t a t g t g g c g c g g t a t t a t c c c g t a t t g a c g c c g g g c a a g a g c a a c t c g g t c g c c g a g a c g a t a c a c c g c g c c a t a a t a g g g c a t a a c t g c g g c c c g t t c t c g t t g a g c c a g c g g c

ScaI 2520 95 100 105 110 IHYSQNDLVEYSPVTEKHLT AmpR IHYSQNDLVEYSPVTEKHLT c a t a c a c t a t t c t c a g a a t g a c t t g g t t g a g t a c t c a c c a g t c a c a g a a a a g c a t c t t a c g t a t g t g a t a a g a g t c t t a c t g a a c c a a c t c a t g a g t g g t c a g t g t c t t t t c g t a g a a t g

2580 115 120 125 130 DGMTVRELCSAAITMSDNTA AmpR DGMTVRELCSAAITMSDNTA g g a t g g c a t g a c a g t a a g a g a a t t a t g c a g t g c t g c c a t a a c c a t g a g t g a t a a c a c t g c c c t a c c g t a c t g t c a t t c t c t t a a t a c g t c a c g a c g g t a t t g g t a c t c a c t a t t g t g a c g

2640 135 140 145 150 ANLLLTTIGGPKELTAFLHN AmpR ANLLLTTIGGPKELTAFLHN g g c c a a c t t a c t t c t g a c a a c g a t c g g a g g a c c g a a g g a g c t a a c c g c t t t t t t g c a c a a c c g g t t g a a t g a a g a c t g t t g c t a g c c t c c t g g c t t c c t c g a t t g g c g a a a a a a c g t g t t

2700 155 160 165 170 MGDHVTRLDRWEPELNEAIP AmpR MGDHVTRLDRWEPELNEAIP c a t g g g g g a t c a t g t a a c t c g c c t t g a t c g t t g g g a a c c g g a g c t g a a t g a a g c c a t a c c g t a c c c c c t a g t a c a t t g a g c g g a a c t a g c a a c c c t t g g c c t c g a c t t a c t t c g g t a t g g

2760 175 180 185 190 NDERDTTMPVAMATTLRKLL AmpR NDERDTTMPVAMATTLRKLL a a a c g a c g a g c g t g a c a c c a c g a t g c c t g t a g c a a t g g c a a c a a c g t t g c g c a a a c t a t t t t t g c t g c t c g c a c t g t g g t g c t a c g g a c a t c g t t a c c g t t g t t g c a a c g c g t t t g a t a a

2820 195 200 205 210 TGELLTLASRQQLIDWMEAD AmpR TGELLTLASRQQLIDWMEAD a a c t g g c g a a c t a c t t a c t c t a g c t t c c c g g c a a c a a t t a a t a g a c t g g a t g g a g g c g g a t t g a c c g c t t g a t g a a t g a g a t c g a a g g g c c g t t g t t a a t t a t c t g a c c t a c c t c c g c c t

NmeAIII 2880 215 220 225 230 KVAGPLLRSALPAGWFIADK AmpR KVAGPLLRSALPAGWFIADK t a a a g t t g c a g g a c c a c t t c t g c g c t c g g c c c t t c c g g c t g g c t g g t t t a t t g c t g a t a a a t t t c a a c g t c c t g g t g a a g a c g c g a g c c g g g a a g g c c g a c c g a c c a a a t a a c g a c t a t t

BpmIBsaI 2940 235 240 245 250 SGAGERGSRGIIAALGPDGK AmpR SGAGERGSRGIIAALGPDGK a t c t g g a g c c g g t g a g c g t g g g t c t c g c g g t a t c a t t g c a g c a c t g g g g c c a g a t g g t a a t a g a c c t c g g c c a c t c g c a c c c a g a g c g c c a t a g t a a c g t c g t g a c c c c g g t c t a c c a t t

AhdI 3000 255 260 265 270 PSRIVVIYTTGSQATMDERN AmpR PSRIVVIYTTGSQATMDERN g c c c t c c c g t a t c g t a g t t a t c t a c a c g a c g g g g a g t c a g g c a a c t a t g g a t g a a c g a a a c g g g a g g g c a t a g c a t c a a t a g a t g t g c t g c c c c t c a g t c c g t t g a t a c c t a c t t g c t t t

3060 275 280 285 RQIAEIGASLIKHW* AmpR RQIAEIGASLIKHW* t a g a c a g a t c g c t g a g a t a g g t g c c t c a c t g a t t a a g c a t t g g t a a c t g t c a g a c c a a g t a t c t g t c t a g c g a c t c t a t c c a c g g a g t g a c t a a t t c g t a a c c a t t g a c a g t c t g g t t c a

3120 t t a c t c a t a t a t a c t t t a g a t t g a t t t a a a a c t t c a t t t t t a a t t t a a a a g g a t c t a g g t a a t g a g t a t a t a t g a a a t c t a a c t a a a t t t t g a a g t a a a a a t t a a a t t t t c c t a g a t c c a

3180 g a a g a t c c t t t t t g a t a a t c t c a t g a c c a a a a t c c c t t a a c g t g a g t t t t c g t t c c a c t g c t t c t a g g a a a a a c t a t t a g a g t a c t g g t t t t a g g g a a t t g c a c t c a a a a g c a a g g t g a c

3240 ori a g c g t c a g a c c c c g t a g a a a a g a t c a a a g g a t c t t c t t g a g a t c c t t t t t t t c t g c g c g t t c g c a g t c t g g g g c a t c t t t t c t a g t t t c c t a g a a g a a c t c t a g g a a a a a a a g a c g c g c a

3300 ori a a t c t g c t g c t t g c a a a c a a a a a a a c c a c c g c t a c c a g c g g t g g t t t g t t t g c c g g a t c a t t a g a c g a c g a a c g t t t g t t t t t t t g g t g g c g a t g g t c g c c a c c a a a c a a a c g g c c t a g t

3360 ori a g a g c t a c c a a c t c t t t t t c c g a a g g t a a c t g g c t t c a g c a g a g c g c a g a t a c c a a a t a c t c t c g a t g g t t g a g a a a a a g g c t t c c a t t g a c c g a a g t c g t c t c g c g t c t a t g g t t t a t g

3420 ori t g t t c t t c t a g t g t a g c c g t a g t t a g g c c a c c a c t t c a a g a a c t c t g t a g c a c c g c c t a c a c a a g a a g a t c a c a t c g g c a t c a a t c c g g t g g t g a a g t t c t t g a g a c a t c g t g g c g g a t g

3480 ori a t a c c t c g c t c t g c t a a t c c t g t t a c c a g t g g c t g c t g c c a g t g g c g a t a a g t c g t g t c t t a t g g a g c g a g a c g a t t a g g a c a a t g g t c a c c g a c g a c g g t c a c c g c t a t t c a g c a c a g a

3540 ori t a c c g g g t t g g a c t c a a g a c g a t a g t t a c c g g a t a a g g c g c a g c g g t c g g g c t g a a c g g g a t g g c c c a a c c t g a g t t c t g c t a t c a a t g g c c t a t t c c g c g t c g c c a g c c c g a c t t g c c c

3600 ori g g g t t c g t g c a c a c a g c c c a g c t t g g a g c g a a c g a c c t a c a c c g a a c t g a g a t a c c t a c a c c c a a g c a c g t g t g t c g g g t c g a a c c t c g c t t g c t g g a t g t g g c t t g a c t c t a t g g a t g t

3650 ori g c g t g a g c t a t g a g a a a g c g c c a c g c t t c c c g a a g g g a g a a a g g c g g a c a c g c a c t c g a t a c t c t t t c g c g g t g c g a a g g g c t t c c c t c t t t c c g c c t g t

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.