Addgene pcDNA5-Flag-BRD4-7D - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pcDNA5-Flag-BRD4-7D


Map View

4000 3000 2000 1000 End (4089) SspI (4067) PluTI (3846) SfoI (3844) NarI * (3843) KasI (3842) SfiI (3820) PpuMI (3576) StuI (3477) MflI * - BstYI (3341) BstEII (3169) AgeI - BsaWI (3166) AleI (2410) SmaI (2193) XmaI - TspMI (2191) XmnI (2089) BsaBI (1957) EcoRI (1935) BsaAI (1793) SacII (1481) XcmI (1434) Bsu36I (1382) BstBI (1349) MscI (1321) TatI (1292) AatII (1265) ZraI (1263) PshAI (709) KpnI (589) Acc65I (585) PflFI - Tth111I (436) BciVI (385) BsmI (366) AccI (79) Start (0) 6xHis pcDNA5-Flag-BRD4-7D 4089 bp

Sequence View

Start (0) 5ʹ 3ʹ 60 MSAESGPGTRLRNLPVMGDG a t g t c t g c g g a g a g c g g c c c t g g g a c g a g a t t g a g a a a t c t g c c a g t a a t g g g g g a t g g a t a c a g a c g c c t c t c g c c g g g a c c c t g c t c t a a c t c t t t a g a c g g t c a t t a c c c c c t a c c t

AccI 120 LETSQMSTTQAQAQPQPANA c t a g a a a c t t c c c a a a t g t c t a c a a c a c a g g c c c a g g c c c a a c c c c a g c c a g c c a a c g c a g a t c t t t g a a g g g t t t a c a g a t g t t g t g t c c g g g t c c g g g t t g g g g t c g g t c g g t t g c g t

180 ASTNPPPPETSNPNKPKRQT g c c a g c a c c a a c c c c c c g c c c c c a g a g a c c t c c a a c c c t a a c a a g c c c a a g a g g c a g a c c c g g t c g t g g t t g g g g g g c g g g g g t c t c t g g a g g t t g g g a t t g t t c g g g t t c t c c g t c t g g

240 NQLQYLLRVVLKTLWKHQFA a a c c a a c t g c a a t a c c t g c t c a g a g t g g t g c t c a a g a c a c t a t g g a a a c a c c a g t t t g c a t t g g t t g a c g t t a t g g a c g a g t c t c a c c a c g a g t t c t g t g a t a c c t t t g t g g t c a a a c g t

300 WPFQQPVDAVKLNLPDYYKI t g g c c t t t c c a g c a g c c t g t g g a t g c c g t c a a g c t g a a c c t c c c t g a t t a c t a t a a g a t c a c c g g a a a g g t c g t c g g a c a c c t a c g g c a g t t c g a c t t g g a g g g a c t a a t g a t a t t c t a g

360 IKTPMDMGTIKKRLENNYYW a t t a a a a c g c c t a t g g a t a t g g g a a c a a t a a a g a a g c g c t t g g a a a a c a a c t a t t a c t g g t a a t t t t g c g g a t a c c t a t a c c c t t g t t a t t t c t t c g c g a a c c t t t t g t t g a t a a t g a c c

BciVIBsmI 420 NAQECIQDFNTMFTNCYIYN a a t g c t c a g g a a t g t a t c c a g g a c t t c a a c a c t a t g t t t a c a a a t t g t t a c a t c t a c a a c t t a c g a g t c c t t a c a t a g g t c c t g a a g t t g t g a t a c a a a t g t t t a a c a a t g t a g a t g t t g

Tth111IPflFI 480 KPGDDIVLMAEALEKLFLQK a a g c c t g g a g a t g a c a t a g t c t t a a t g g c a g a a g c t c t g g a a a a g c t c t t c t t g c a a a a a t t c g g a c c t c t a c t g t a t c a g a a t t a c c g t c t t c g a g a c c t t t t c g a g a a g a a c g t t t t t

540 INELPTEETEIMIVQAKGRG a t a a a t g a g c t a c c c a c a g a a g a a a c c g a g a t c a t g a t a g t c c a g g c a a a a g g a a g a g g a t a t t t a c t c g a t g g g t g t c t t c t t t g g c t c t a g t a c t a t c a g g t c c g t t t t c c t t c t c c t

Acc65IKpnI 600 RGRKETGTAKPGVSTVPNTT c g t g g g a g g a a a g a a a c a g g g a c a g c a a a a c c t g g c g t t t c c a c g g t a c c a a a c a c a a c t g c a c c c t c c t t t c t t t g t c c c t g t c g t t t t g g a c c g c a a a g g t g c c a t g g t t t g t g t t g a

660 QASTPPQTQTPQPNPPPVQA c a a g c a t c g a c t c c t c c g c a g a c c c a g a c c c c t c a g c c g a a t c c t c c t c c t g t g c a g g c c g t t c g t a g c t g a g g a g g c g t c t g g g t c t g g g g a g t c g g c t t a g g a g g a g g a c a c g t c c g g

PshAI 720 TPHPFPAVTPDLIVQTPVMT a c g c c t c a c c c c t t c c c t g c c g t c a c c c c g g a c c t c a t c g t c c a g a c c c c t g t c a t g a c a t g c g g a g t g g g g a a g g g a c g g c a g t g g g g c c t g g a g t a g c a g g t c t g g g g a c a g t a c t g t

780 VVPPQPLQTPPPVPPQPQPP g t g g t g c c t c c c c a g c c a c t g c a g a c g c c c c c g c c a g t g c c c c c c c a g c c a c a a c c c c c a c a c c a c g g a g g g g t c g g t g a c g t c t g c g g g g g c g g t c a c g g g g g g g t c g g t g t t g g g g g t

840 PAPAPQPVQSHPPIIAATPQ c c c g c t c c a g c t c c c c a g c c c g t a c a g a g c c a c c c a c c c a t c a t c g c g g c c a c c c c a c a g g g g c g a g g t c g a g g g g t c g g g c a t g t c t c g g t g g g t g g g t a g t a g c g c c g g t g g g g t g t c

900 PVKTKKGVKRKADTTTPTTI c c t g t g a a g a c a a a g a a g g g a g t g a a g a g g a a a g c a g a c a c c a c c a c c c c c a c c a c c a t t g g a c a c t t c t g t t t c t t c c c t c a c t t c t c c t t t c g t c t g t g g t g g t g g g g g t g g t g g t a a

960 DPIHEPPSLPPEPKTTKLGQ g a c c c c a t t c a c g a g c c a c c c t c g c t g c c c c c g g a g c c c a a g a c c a c c a a g c t g g g c c a g c t g g g g t a a g t g c t c g g t g g g a g c g a c g g g g g c c t c g g g t t c t g g t g g t t c g a c c c g g t c

1020 RRESSRPVKPPKKDVPDSQQ c g g c g g g a g a g c a g c c g g c c t g t g a a a c c t c c a a a g a a g g a c g t g c c c g a c t c t c a g c a g g c c g c c c t c t c g t c g g c c g g a c a c t t t g g a g g t t t c t t c c t g c a c g g g c t g a g a g t c g t c

1080 HPAPEKSSKVSEQLKCCSGI c a c c c a g c a c c a g a g a a g a g c a g c a a g g t c t c g g a g c a g c t c a a g t g c t g c a g c g g c a t c g t g g g t c g t g g t c t c t t c t c g t c g t t c c a g a g c c t c g t c g a g t t c a c g a c g t c g c c g t a g

1140 LKEMFAKKHAAYAWPFYKPV c t c a a g g a g a t g t t t g c c a a g a a g c a c g c c g c c t a c g c c t g g c c c t t c t a c a a g c c t g t g g a g t t c c t c t a c a a a c g g t t c t t c g t g c g g c g g a t g c g g a c c g g g a a g a t g t t c g g a c a c

1200 DVEALGLHDYCDIIKHPMDM g a c g t g g a g g c a c t g g g c c t a c a c g a c t a c t g t g a c a t c a t c a a g c a c c c c a t g g a c a t g c t g c a c c t c c g t g a c c c g g a t g t g c t g a t g a c a c t g t a g t a g t t c g t g g g g t a c c t g t a c

1260 STIKSKLEAREYRDAQEFGA a g c a c a a t c a a g t c t a a a c t g g a g g c c c g t g a g t a c c g t g a t g c t c a g g a g t t t g g t g c t t c g t g t t a g t t c a g a t t t g a c c t c c g g g c a c t c a t g g c a c t a c g a g t c c t c a a a c c a c g a

TatIZraIAatII 1320 DVRLMFSNCYKYNPPDHEVV g a c g t c c g a t t g a t g t t c t c c a a c t g c t a t a a g t a c a a c c c t c c t g a c c a t g a g g t g g t g c t g c a g g c t a a c t a c a a g a g g t t g a c g a t a t t c a t g t t g g g a g g a c t g g t a c t c c a c c a c

BstBIMscI 1380 AMARKLQDVFEMRFAKMPDE g c c a t g g c c c g c a a g c t c c a g g a t g t g t t c g a a a t g c g c t t t g c c a a g a t g c c g g a c g a g c g g t a c c g g g c g t t c g a g g t c c t a c a c a a g c t t t a c g c g a a a c g g t t c t a c g g c c t g c t c

XcmIBsu36I 1440 PEEPVVAVSSPAVPPPTKVV c c t g a g g a g c c a g t g g t g g c c g t g t c c t c c c c g g c a g t g c c c c c t c c c a c c a a g g t t g t g g g a c t c c t c g g t c a c c a c c g g c a c a g g a g g g g c c g t c a c g g g g g a g g g t g g t t c c a a c a c

SacII 1500 APPDSSDDSSDASADSDAAT g c c c c g c c c g a t t c c a g c g a c g a c a g c a g c g a t g c c t c c g c g g a c a g t g a c g c t g c g a c t c g g g g c g g g c t a a g g t c g c t g c t g t c g t c g c t a c g g a g g c g c c t g t c a c t g c g a c g c t g a

1560 DDAEEERAQRLAELQEQLKA g a t g a c g c t g a g g a g g a g c g a g c c c a g c g g c t g g c t g a g c t c c a g g a g c a g c t c a a a g c c c t a c t g c g a c t c c t c c t c g c t c g g g t c g c c g a c c g a c t c g a g g t c c t c g t c g a g t t t c g g

1620 VHEQLAALSQPQQNKPKKKE g t g c a c g a g c a g c t t g c a g c c c t c t c t c a g c c c c a g c a g a a c a a a c c a a a g a a a a a g g a g c a c g t g c t c g t c g a a c g t c g g g a g a g a g t c g g g g t c g t c t t g t t t g g t t t c t t t t t c c t c

1680 KDKKEKKKEKHKRKEEVEEN a a a g a c a a g a a g g a a a a g a a a a a a g a a a a g c a c a a a a g g a a a g a g g a a g t g g a a g a g a a t t t t c t g t t c t t c c t t t t c t t t t t t c t t t t c g t g t t t t c c t t t c t c c t t c a c c t t c t c t t a

1740 KKSKAKEPPPKKTKKNNSSN a a a a a a a g c a a a g c c a a g g a a c c t c c t c c t a a a a a g a c g a a g a a a a a t a a t a g c a g c a a c t t t t t t t c g t t t c g g t t c c t t g g a g g a g g a t t t t t c t g c t t c t t t t t a t t a t c g t c g t t g

BsaAI 1800 SNVSKKEPAPMKSKPPPTYE a g c a a t g t g a g c a a g a a g g a g c c a g c g c c c a t g a a g a g c a a g c c c c c t c c c a c g t a t g a g t c g t t a c a c t c g t t c t t c c t c g g t c g c g g g t a c t t c t c g t t c g g g g g a g g g t g c a t a c t c

1860 SEEEDKCKPMSYEEKRQLSL t c g g a g g a a g a g g a c a a g t g c a a g c c t a t g t c c t a t g a g g a g a a g c g g c a g c t c a g c t t g a g c c t c c t t c t c c t g t t c a c g t t c g g a t a c a g g a t a c t c c t c t t c g c c g t c g a g t c g a a c

1920 DINKLPGEKLGRVVHIIQSR g a c a t c a a c a a g c t c c c c g g c g a g a a g c t g g g c c g c g t g g t g c a c a t c a t c c a g t c a c g g c t g t a g t t g t t c g a g g g g c c g c t c t t c g a c c c g g c g c a c c a c g t g t a g t a g g t c a g t g c c

BsaBIEcoRI 1980 EPSLKNSNPDEIEIDFETLK g a g c c c t c c c t g a a g a a t t c c a a c c c c g a c g a g a t t g a a a t c g a c t t t g a g a c c c t g a a g c t c g g g a g g g a c t t c t t a a g g t t g g g g c t g c t c t a a c t t t a g c t g a a a c t c t g g g a c t t c

2040 PSTLRELERYVTSCLRKKRK c c g t c c a c a c t g c g t g a g c t g g a g c g c t a t g t c a c c t c c t g t t t g c g g a a g a a a a g g a a a g g c a g g t g t g a c g c a c t c g a c c t c g c g a t a c a g t g g a g g a c a a a c g c c t t c t t t t c c t t t

XmnI 2100 PQAEKVDVIAGSSKMKGFSS c c t c a a g c t g a g a a a g t t g a t g t g a t t g c c g g c t c c t c c a a g a t g a a g g g c t t c t c g t c c g g a g t t c g a c t c t t t c a a c t a c a c t a a c g g c c g a g g a g g t t c t a c t t c c c g a a g a g c a g g

2160 SESESSSESSSSDSEDSETE t c a g a g t c g g a g a g c t c c a g t g a g t c c a g c t c c t c t g a c a g c g a a g a c t c c g a a a c a g a g a g t c t c a g c c t c t c g a g g t c a c t c a g g t c g a g g a g a c t g t c g c t t c t g a g g c t t t g t c t c

TspMIXmaISmaI 2220 1 HHH 6xHis MAPKSKKKGHPGREQKKHHH a t g g c t c c g a a g t c a a a a a a g a a g g g g c a c c c c g g g a g g g a g c a g a a g a a g c a c c a t c a t t a c c g a g g c t t c a g t t t t t t c t t c c c c g t g g g g c c c t c c c t c g t c t t c t t c g t g g t a g t a

2280 5 HHH (in frame with 6xHis) QQMQQAPAPVPQQPPPP 6xHis HHHQQMQQAPAPVPQQPPPP c a c c a c c a t c a g c a g a t g c a g c a g g c c c c g g c t c c t g t g c c c c a g c a g c c g c c c c c g c c t g t g g t g g t a g t c g t c t a c g t c g t c c g g g g c c g a g g a c a c g g g g t c g t c g g c g g g g g c g g a

2340 (in frame with 6xHis) PQQPPPPPPPQQQQQPPPPP PQQPPPPPPPQQQQQPPPPP c c c c a g c a g c c c c c a c c g c c t c c a c c t c c g c a g c a g c a a c a g c a g c c g c c a c c c c c g c c t g g g g t c g t c g g g g g t g g c g g a g g t g g a g g c g t c g t c g t t g t c g t c g g c g g t g g g g g c g g a

2400 (in frame with 6xHis) PPPSMPQQAAPAMKSSPPPF PPPSMPQQAAPAMKSSPPPF c c c c c a c c c t c c a t g c c g c a g c a g g c a g c c c c g g c g a t g a a g t c c t c g c c c c c a c c c t t c g g g g g t g g g a g g t a c g g c g t c g t c c g t c g g g g c c g c t a c t t c a g g a g c g g g g g t g g g a a g

AleI 2460 (in frame with 6xHis) IATQVPVLEPQLPGSVFDPI IATQVPVLEPQLPGSVFDPI a t t g c c a c c c a g g t g c c c g t c c t g g a g c c c c a g c t c c c a g g c a g c g t c t t t g a c c c c a t c t a a c g g t g g g t c c a c g g g c a g g a c c t c g g g g t c g a g g g t c c g t c g c a g a a a c t g g g g t a g

2520 (in frame with 6xHis) GHFTQPILHLPQPELPPHLP GHFTQPILHLPQPELPPHLP g g c c a c t t c a c c c a g c c c a t c c t g c a c c t g c c g c a g c c t g a g c t g c c c c c t c a c c t g c c c c c g g t g a a g t g g g t c g g g t a g g a c g t g g a c g g c g t c g g a c t c g a c g g g g g a g t g g a c g g g

2580 (in frame with 6xHis) QPPEHSTPPHLNQHAVVSPP QPPEHSTPPHLNQHAVVSPP c a g c c g c c t g a g c a c a g c a c t c c a c c c c a t c t c a a c c a g c a c g c a g t g g t c t c t c c t c c a g t c g g c g g a c t c g t g t c g t g a g g t g g g g t a g a g t t g g t c g t g c g t c a c c a g a g a g g a g g t

2640 (in frame with 6xHis) ALHNALPQQPSRPSNRAAAL ALHNALPQQPSRPSNRAAAL g c t t t g c a c a a c g c a c t a c c c c a g c a g c c a t c a c g g c c c a g c a a c c g a g c c g c t g c c c t g c g a a a c g t g t t g c g t g a t g g g g t c g t c g g t a g t g c c g g g t c g t t g g c t c g g c g a c g g g a c

2700 (in frame with 6xHis) PPKPARPPAVSPALTQTPLL PPKPARPPAVSPALTQTPLL c c t c c c a a g c c c g c c c g g c c c c c a g c c g t g t c a c c a g c c t t g a c c c a a a c a c c c c t g c t c g g a g g g t t c g g g c g g g c c g g g g g t c g g c a c a g t g g t c g g a a c t g g g t t t g t g g g g a c g a g

2760 (in frame with 6xHis) PQPPMAQPPQVLLEDEEPPA PQPPMAQPPQVLLEDEEPPA c c a c a g c c c c c c a t g g c c c a a c c c c c c c a a g t g c t g c t g g a g g a t g a a g a g c c a c c t g c c g g t g t c g g g g g g t a c c g g g t t g g g g g g g t t c a c g a c g a c c t c c t a c t t c t c g g t g g a c g g

2820 (in frame with 6xHis) PPLTSMQMQLYLQQLQKVQP PPLTSMQMQLYLQQLQKVQP c c a c c c c t c a c c t c c a t g c a g a t g c a g c t g t a c c t g c a g c a g c t g c a g a a g g t g c a g c c c g g t g g g g a g t g g a g g t a c g t c t a c g t c g a c a t g g a c g t c g t c g a c g t c t t c c a c g t c g g g

2880 (in frame with 6xHis) PTPLLPSVKVQSQPPPPLPP PTPLLPSVKVQSQPPPPLPP c c t a c g c c g c t a c t c c c t t c c g t g a a g g t g c a g t c c c a g c c c c c a c c c c c c c t g c c g c c c g g a t g c g g c g a t g a g g g a a g g c a c t t c c a c g t c a g g g t c g g g g g t g g g g g g g a c g g c g g g

2940 (in frame with 6xHis) PPHPSVQQQLQQQPPPPPPP PPHPSVQQQLQQQPPPPPPP c c a c c c c a c c c c t c t g t g c a g c a g c a g c t g c a g c a g c a g c c g c c a c c a c c c c c a c c a c c c g g t g g g g t g g g g a g a c a c g t c g t c g t c g a c g t c g t c g t c g g c g g t g g t g g g g g t g g t g g g

3000 (in frame with 6xHis) QPQPPPQQQHQPPPRPVHLQ QPQPPPQQQHQPPPRPVHLQ c a g c c c c a g c c t c c a c c c c a g c a g c a g c a t c a g c c c c c t c c a c g g c c c g t g c a c t t g c a g g t c g g g g t c g g a g g t g g g g t c g t c g t c g t a g t c g g g g g a g g t g c c g g g c a c g t g a a c g t c

3060 (in frame with 6xHis) PMQFSTHIQQPPPPQGQQPP PMQFSTHIQQPPPPQGQQPP c c c a t g c a g t t t t c c a c c c a c a t c c a a c a g c c c c c g c c a c c c c a g g g c c a g c a g c c c c c c g g g t a c g t c a a a a g g t g g g t g t a g g t t g t c g g g g g c g g t g g g g t c c c g g t c g t c g g g g g g

3120 (in frame with 6xHis) HPPPGQQPPPPQPAKPQQVI HPPPGQQPPPPQPAKPQQVI c a t c c g c c c c c a g g c c a g c a g c c a c c c c c g c c g c a g c c t g c c a a g c c t c a g c a a g t c a t c g t a g g c g g g g g t c c g g t c g t c g g t g g g g g c g g c g t c g g a c g g t t c g g a g t c g t t c a g t a g

BsaWIAgeIBstEII 3180 (in frame with 6xHis) QHHHSPRHHKSDPYSTGHLR QHHHSPRHHKSDPYSTGHLR c a g c a c c a c c a t t c a c c c c g g c a c c a c a a g t c g g a c c c c t a c t c a a c c g g t c a c c t c c g c g t c g t g g t g g t a a g t g g g g c c g t g g t g t t c a g c c t g g g g a t g a g t t g g c c a g t g g a g g c g

3240 (in frame with 6xHis) EAPSPLMIHSPQMSQFQSLT EAPSPLMIHSPQMSQFQSLT g a a g c c c c c t c c c c g c t t a t g a t a c a t t c c c c c c a g a t g t c a c a g t t c c a g a g c c t g a c c c t t c g g g g g a g g g g c g a a t a c t a t g t a a g g g g g g t c t a c a g t g t c a a g g t c t c g g a c t g g

3300 (in frame with 6xHis) HQSPPQQNVQPKKQELRAAS HQSPPQQNVQPKKQELRAAS c a c c a g t c t c c a c c c c a g c a a a a c g t c c a g c c t a a g a a a c a g g a g c t g c g t g c t g c c t c c g t g g t c a g a g g t g g g g t c g t t t t g c a g g t c g g a t t c t t t g t c c t c g a c g c a c g a c g g a g g

BstYIMflI* 3360 (in frame with 6xHis) VVQPQPLVVVKEEKIHSPII VVQPQPLVVVKEEKIHSPII g t g g t c c a g c c c c a g c c c c t c g t g g t g g t g a a g g a g g a g a a g a t c c a c t c a c c c a t c a t c c a c c a g g t c g g g g t c g g g g a g c a c c a c c a c t t c c t c c t c t t c t a g g t g a g t g g g t a g t a g

3420 (in frame with 6xHis) RSEPFSPSLRPEPPKHPESI RSEPFSPSLRPEPPKHPESI c g c a g c g a g c c c t t c a g c c c c t c g c t g c g g c c g g a g c c c c c c a a g c a c c c g g a g a g c a t c g c g t c g c t c g g g a a g t c g g g g a g c g a c g c c g g c c t c g g g g g g t t c g t g g g c c t c t c g t a g

StuI 3480 (in frame with 6xHis) KAPVHLPQRPEMKPVDVGRP KAPVHLPQRPEMKPVDVGRP a a g g c c c c c g t c c a c c t g c c c c a g c g g c c g g a a a t g a a g c c t g t g g a t g t c g g g a g g c c t t t c c g g g g g c a g g t g g a c g g g g t c g c c g g c c t t t a c t t c g g a c a c c t a c a g c c c t c c g g a

3540 (in frame with 6xHis) VIRPPEQNAPPPGAPDKDKQ VIRPPEQNAPPPGAPDKDKQ g t g a t c c g g c c c c c a g a g c a g a a c g c a c c g c c a c c a g g g g c c c c t g a c a a g g a c a a a c a g c a c t a g g c c g g g g g t c t c g t c t t g c g t g g c g g t g g t c c c c g g g g a c t g t t c c t g t t t g t c

PpuMI 3600 (in frame with 6xHis) KQEPKTPVAPKKDLKIKNMG KQEPKTPVAPKKDLKIKNMG a a a c a g g a g c c g a a g a c t c c a g t t g c g c c c a a a a a g g a c c t g a a a a t c a a g a a c a t g g g c t t t g t c c t c g g c t t c t g a g g t c a a c g c g g g t t t t t c c t g g a c t t t t a g t t c t t g t a c c c g

3660 (in frame with 6xHis) SWASLVQKHPTTPSSTAKSS SWASLVQKHPTTPSSTAKSS t c c t g g g c c a g c c t a g t g c a g a a g c a t c c g a c c a c c c c c t c c t c c a c a g c c a a g t c a t c c a g g a c c c g g t c g g a t c a c g t c t t c g t a g g c t g g t g g g g g a g g a g g t g t c g g t t c a g t a g g

3720 (in frame with 6xHis) SDSFEQFRRAAREKEEREKA SDSFEQFRRAAREKEEREKA a g c g a c a g c t t c g a g c a g t t c c g c c g c g c c g c t c g g g a g a a a g a g g a g c g t g a g a a g g c c t c g c t g t c g a a g c t c g t c a a g g c g g c g c g g c g a g c c c t c t t t c t c c t c g c a c t c t t c c g g

3780 (in frame with 6xHis) LKAQAEHAEKEKERLRQERM LKAQAEHAEKEKERLRQERM c t g a a g g c t c a g g c c g a g c a c g c t g a g a a g g a g a a g g a g c g g c t g c g g c a g g a g c g c a t g g a c t t c c g a g t c c g g c t c g t g c g a c t c t t c c t c t t c c t c g c c g a c g c c g t c c t c g c g t a c

SfiI 3840 (in frame with 6xHis) RSREDEDALEQARRAHEEAR RSREDEDALEQARRAHEEAR a g g a g c c g a g a g g a c g a g g a t g c g c t g g a g c a g g c c c g g c g g g c c c a t g a g g a g g c a c g t t c c t c g g c t c t c c t g c t c c t a c g c g a c c t c g t c c g g g c c g c c c g g g t a c t c c t c c g t g c a

KasINarI*SfoIPluTI 3900 (in frame with 6xHis) RRQEQQQQQRQEQQQQQQQQ RRQEQQQQQRQEQQQQQQQQ c g g c g c c a g g a g c a g c a g c a g c a g c a g c g c c a g g a g c a a c a g c a g c a g c a g c a a c a g c a a g c c g c g g t c c t c g t c g t c g t c g t c g t c g c g g t c c t c g t t g t c g t c g t c g t c g t t g t c g t t

3960 (in frame with 6xHis) AAAVAAAATPQAQSSQPQSM AAAVAAAATPQAQSSQPQSM g c a g c t g c g g t g g c t g c c g c c g c c a c c c c a c a g g c c c a g a g c t c c c a g c c c c a g t c c a t g c g t c g a c g c c a c c g a c g g c g g c g g t g g g g t g t c c g g g t c t c g a g g g t c g g g g t c a g g t a c

4020 (in frame with 6xHis) LDQQRELARKREQERRRREA LDQQRELARKREQERRRREA c t g g a c c a g c a g a g g g a g t t g g c c c g g a a g c g g g a g c a g g a g c g a a g a c g c c g g g a a g c c g a c c t g g t c g t c t c c c t c a a c c g g g c c t t c g c c c t c g t c c t c g c t t c t g c g g c c c t t c g g

SspI 4080 (in frame with 6xHis) MAATIDMNFQSDLLSIFEEN MAATIDMNFQSDLLSIFEEN a t g g c a g c t a c c a t t g a c a t g a a t t t c c a g a g t g a t c t a t t g t c a a t a t t t g a a g a a a a t t a c c g t c g a t g g t a a c t g t a c t t a a a g g t c t c a c t a g a t a a c a g t t a t a a a c t t c t t t t a

End (4089) 3ʹ 5ʹ 4089 LF* LF* c t t t t c t g a g a a a a g a c t

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.