Addgene HOXB1 (human) HIS-tag pET Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: HOXB1 (human) HIS-tag pET Sequencing Result

Map View

800 600 400 200 End (867) SacI - BsiHKAI (859) Eco53kI (857) PvuII (776) BmrI (736) StuI * (730) AcuI - Eco57MI (625) EcoNI (615) BseRI (600) RsrII (531) BfuAI PaqCI BspMI (510) SmlI XhoI PspXI PaeR7I (473) Esp3I - BsmBI (445) ScaI (426) TatI (424) PstI (402) PasI (347) HincII (251) NmeAIII (217) KflI - PpuMI (186) XmnI (156) MflI * - BstYI - BamHI (129) BmtI (100) NheI (96) NdeI (91) SspI (3) Start (0) 6xHis thrombin site T7 tag (gene 10 leader) HOXB1 (human) HIS-tag pET 867 bp

Sequence View

Start (0) SspI 5ʹ 3ʹ 60 1 5 HHHHH 6xHis YFVYFRRRYTMGSSHHHHH a a t a t t t t g t t t a c t t t a g a a g g a g a t a t a c c a t g g g c a g c a g c c a t c a t c a t c a t c a t c t t a t a a a a c a a a t g a a a t c t t c c t c t a t a t g g t a c c c g t c g t c g g t a g t a g t a g t a g t a g

BmtINheINdeI 120 H SSG 1 5 LVPRGS H 1 5 MASMTGGQQ 6xHis thrombin site T7 tag (gene 10 leader) HSSGLVPRGSHMASMTGGQQ a c a g c a g c g g c c t g g t g c c g c g c g g c a g c c a t a t g g c t a g c a t g a c t g g t g g a c a g c a a a t g t c g t c g c c g g a c c a c g g c g c g c c g t c g g t a t a c c g a t c g t a c t g a c c a c c t g t c g t t t

XmnIBamHIBstYIMflI* 180 10 MG (in frame with T7 tag (gene 10 leader)) RGSMDYNRMNSFLEYPLC T7 tag (gene 10 leader) MGRGSMDYNRMNSFLEYPLC t g g g t c g c g g a t c c a t g g a c t a t a a t a g g a t g a a c t c c t t c t t a g a g t a c c c a c t c t g t a a c c c a g c g c c t a g g t a c c t g a t a t t a t c c t a c t t g a g g a a g a a t c t c a t g g g t g a g a c a t

NmeAIIIPpuMIKflI 240 (in frame with T7 tag (gene 10 leader)) NRGPSAYSAHSAPTSFPPSS NRGPSAYSAHSAPTSFPPSS a c c g g g g a c c c a g c g c c t a c a g c g c c c a c a g c g c c c c a a c c t c c t t t c c c c c a a g c t c g g t g g c c c c t g g g t c g c g g a t g t c g c g g g t g t c g c g g g g t t g g a g g a a a g g g g g t t c g a g c c

HincII 300 (in frame with T7 tag (gene 10 leader)) AQAVDSYASEGRYGGGLSSP AQAVDSYASEGRYGGGLSSP c t c a g g c g g t t g a c a g c t a t g c a a g c g a g g g c c g c t a c g g t g g g g g g c t g t c c a g c c c t g g a g t c c g c c a a c t g t c g a t a c g t t c g c t c c c g g c g a t g c c a c c c c c c g a c a g g t c g g g a c

PasI 360 (in frame with T7 tag (gene 10 leader)) AFQQNSGYPAQQPPSTLGVP AFQQNSGYPAQQPPSTLGVP c g t t t c a g c a g a a c t c c g g c t a t c c c g c c c a g c a g c c g c c t t c g a c c c t g g g g g t g c c c t g c a a a g t c g t c t t g a g g c c g a t a g g g c g g g t c g t c g g c g g a a g c t g g g a c c c c c a c g g g a

PstI 420 (in frame with T7 tag (gene 10 leader)) FPSSAPSGYAPAACSPSYGP FPSSAPSGYAPAACSPSYGP t c c c c a g c t c c g c g c c c t c g g g g t a t g c t c c t g c c g c c t g c a g c c c c a g c t a c g g g c c t t a g g g g t c g a g g c g c g g g a g c c c c a t a c g a g g a c g g c g g a c g t c g g g g t c g a t g c c c g g a a

PaeR7IPspXIXhoISmlIBsmBIEsp3ITatIScaI 480 (in frame with T7 tag (gene 10 leader)) SQYYPLGQSEGDGGYFHPSS SQYYPLGQSEGDGGYFHPSS c t c a g t a c t a c c c t c t g g g t c a a t c a g a a g g a g a c g g a g g c t a t t t t c a t c c c t c g a g c t g a g t c a t g a t g g g a g a c c c a g t t a g t c t t c c t c t g c c t c c g a t a a a a g t a g g g a g c t c g a

RsrIIBspMIPaqCIBfuAI 540 (in frame with T7 tag (gene 10 leader)) YGAQLGGLSDGYGAGGAGPG YGAQLGGLSDGYGAGGAGPG a c g g g g c c c a g c t a g g g g g c t t g t c c g a t g g c t a c g g a g c a g g t g g a g c c g g t c c g g g g c t g c c c c g g g t c g a t c c c c c g a a c a g g c t a c c g a t g c c t c g t c c a c c t c g g c c a g g c c c c g

BseRI 600 (in frame with T7 tag (gene 10 leader)) PYPPQHPPYGNEQTASFAPA PYPPQHPPYGNEQTASFAPA c a t a t c c t c c g c a g c a t c c c c c t t a t g g g a a c g a g c a g a c c g c g a g c t t t g c a c c g g c c t g t a t a g g a g g c g t c g t a g g g g g a a t a c c c t t g c t c g t c t g g c g c t c g a a a c g t g g c c g g a

Eco57MIAcuIEcoNI 660 (in frame with T7 tag (gene 10 leader)) YADLLSEDKETPCPSEPNTP YADLLSEDKETPCPSEPNTP a t g c t g a t c t c c t c t c c g a g g a c a a g g a a a c a c c c t g c c c t t c a g a a c c t a a c a c c c c c a t a c g a c t a g a g g a g a g g c t c c t g t t c c t t t g t g g g a c g g g a a g t c t t g g a t t g t g g g g g t

720 (in frame with T7 tag (gene 10 leader)) TARTFDWMKVKRNPPKTAKV TARTFDWMKVKRNPPKTAKV c g g c c c g g a c c t t c g a c t g g a t g a a g g t t a a g a g a a a c c c a c c c a a g a c a g c g a a g g t g t g c c g g g c c t g g a a g c t g a c c t a c t t c c a a t t c t c t t t g g g t g g g t t c t g t c g c t t c c a c a

PvuIIBmrIStuI* 780 (in frame with T7 tag (gene 10 leader)) SEPGLGSPSGLRTNFTTRQL SEPGLGSPSGLRTNFTTRQL c a g a g c c a g g c c t g g g c t c g c c c a g t g g c c t c c g c a c c a a c t t c a c c a c a a g g c a g c t g a g t c t c g g t c c g g a c c c g a g c g g g t c a c c g g a g g c g t g g t t g a a g t g g t g t t c c g t c g a c t

840 (in frame with T7 tag (gene 10 leader)) TELEKEFHFNKYLSRARRVE TELEKEFHFNKYLSRARRVE c a g a a c t g g a a a a g g a g t t c c a t t t c a a c a a g t a c c t g a g c c g g g c c c g g a g g g t g g a g a g t c t t g a c c t t t t c c t c a a g g t a a a g t t g t t c a t g g a c t c g g c c c g g g c c t c c c a c c t c t

End (867) Eco53kIBsiHKAISacI 3ʹ 5ʹ 867 (in frame with T7 tag (gene 10 leader)) IAATLELNE IAATLELNE t t g c c g c c a c c c t g g a g c t c a a t g a a c a a c g g c g g t g g g a c c t c g a g t t a c t t g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.