Addgene Ubiquitin K6C/G76C Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: Ubiquitin K6C/G76C Sequencing Result


Map View

MscI * (5835) FspAI (5825) pGEX 3' (5160 .. 5182) PflFI - Tth111I (5060) BspQI - SapI (4979) L4440 (4960 .. 4977) PciI (4862) pBR322ori-F (4707 .. 4726) Bsu36I (4173) BglI (3939) ScaI (3576) TatI (3574) Amp-R (3488 .. 3507) DraI (3479) AmpR promoter BspHI (3217) RsrII (3173) rrnB T2 terminator rrnB T1 terminator HindIII (2940) PflMI (13) lacIq promoter LacI-R (100 .. 119) MluI (431) BstEII (612) PspOMI (638) ApaI (642) KasI (1070) NarI * (1071) SfoI (1072) PluTI (1074) M13/pUC Reverse (1447 .. 1469) lac operator MfeI (1502) BsiWI (1836) PsiI (1898) BmgBI (2088) BsmI (2166) BlpI (2332) MBP-F (2620 .. 2643) Eco53kI (2654) SacI (2656) TEV site AgeI (2704) XmnI (2783) AvaI - BsoBI - PaeR7I - PspXI - XhoI (2827) SalI (2871) AccI (2872) AflII (2889) BamHI (2911) PstI - SbfI (2928) Ubiquitin K6C/G76C 5860 bp

Sequence View

PflMI 5ʹ 3ʹ 60 lacIq promoter c c g a c a c c a t c g a a t g g t g c a a a a c c t t t c g c g g t a t g g c a t g a t a g c g c c c g g a a g a g a g g c t g t g g t a g c t t a c c a c g t t t t g g a a a g c g c c a t a c c g t a c t a t c g c g g g c c t t c t c t

120 1 5 10 MKPVTLYDVAEYA lacIq promoter lacI T G C T A C A G C G T C T C A T A C G G LacI-R g t c a a t t c a g g g t g g t g a a t g t g a a a c c a g t a a c g t t a t a c g a t g t c g c a g a g t a t g c c g c a g t t a a g t c c c a c c a c t t a c a c t t t g g t c a t t g c a a t a t g c t a c a g c g t c t c a t a c g g c

180 15 20 25 30 GVSYQTVSRVVNQASHVSAK lacI g t g t c t c t t a t c a g a c c g t t t c c c g c g t g g t g a a c c a g g c c a g c c a c g t t t c t g c g a a a a c a c a g a g a a t a g t c t g g c a a a g g g c g c a c c a c t t g g t c c g g t c g g t g c a a a g a c g c t t t t

240 35 40 45 50 TREKVEAAMAELNYIPNRVA lacI MAELNYIPNRVA c g c g g g a a a a a g t g g a a g c g g c g a t g g c g g a g c t g a a t t a c a t t c c c a a c c g c g t g g c a c g c g c c c t t t t t c a c c t t c g c c g c t a c c g c c t c g a c t t a a t g t a a g g g t t g g c g c a c c g t g

300 55 60 65 70 QQLAGKQSLLIGVATSSLAL lacI QQLAGKQSLLIGVATSSLAL a a c a a c t g g c g g g c a a a c a g t c g t t g c t g a t t g g c g t t g c c a c c t c c a g t c t g g c c c t g c t t g t t g a c c g c c c g t t t g t c a g c a a c g a c t a a c c g c a a c g g t g g a g g t c a g a c c g g g a c g

360 75 80 85 90 HAPSQIVAAIKSRADQLGAS lacI HAPSQIVAAIKSRADQLGAS a c g c g c c g t c g c a a a t t g t c g c g g c g a t t a a a t c t c g c g c c g a t c a a c t g g g t g c c a g c g t g c g c g g c a g c g t t t a a c a g c g c c g c t a a t t t a g a g c g c g g c t a g t t g a c c c a c g g t c g c

420 95 100 105 110 VVVSMVERSGVEACKAAVHN lacI VVVSMVERSGVEACKAAVHN t g g t g g t g t c g a t g g t a g a a c g a a g c g g c g t c g a a g c c t g t a a a g c g g c g g t g c a c a a t c a c c a c c a c a g c t a c c a t c t t g c t t c g c c g c a g c t t c g g a c a t t t c g c c g c c a c g t g t t a g

MluI 480 115 120 125 130 LLAQRVSGLIINYPLDDQDA lacI LLAQRVSGLIINYPLDDQDA t t c t c g c g c a a c g c g t c a g t g g g c t g a t c a t t a a c t a t c c g c t g g a t g a c c a g g a t g c c a a a g a g c g c g t t g c g c a g t c a c c c g a c t a g t a a t t g a t a g g c g a c c t a c t g g t c c t a c g g t

540 135 140 145 150 IAVEAACTNVPALFLDVSDQ lacI IAVEAACTNVPALFLDVSDQ t t g c t g t g g a a g c t g c c t g c a c t a a t g t t c c g g c g t t a t t t c t t g a t g t c t c t g a c c a g a a a c g a c a c c t t c g a c g g a c g t g a t t a c a a g g c c g c a a t a a a g a a c t a c a g a g a c t g g t c t

600 155 160 165 170 TPINSIIFSHEDGTRLGVEH lacI TPINSIIFSHEDGTRLGVEH c a c c c a t c a a c a g t a t t a t t t t c t c c c a t g a a g a c g g t a c g c g a c t g g g c g t g g a g c a t c g t g g g t a g t t g t c a t a a t a a a a g a g g g t a c t t c t g c c a t g c g c t g a c c c g c a c c t c g t a g

PspOMIApaIBstEII 660 175 180 185 190 LVALGHQQIALLAGPLSSVS lacI LVALGHQQIALLAGPLSSVS t g g t c g c a t t g g g t c a c c a g c a a a t c g c g c t g t t a g c g g g c c c a t t a a g t t c t g t c t c g g a c c a g c g t a a c c c a g t g g t c g t t t a g c g c g a c a a t c g c c c g g g t a a t t c a a g a c a g a g c c

720 195 200 205 210 ARLRLAGWHKYLTRNQIQPI lacI ARLRLAGWHKYLTRNQIQPI c g c g t c t g c g t c t g g c t g g c t g g c a t a a a t a t c t c a c t c g c a a t c a a a t t c a g c c g a t a g g c g c a g a c g c a g a c c g a c c g a c c g t a t t t a t a g a g t g a g c g t t a g t t t a a g t c g g c t a t c

780 215 220 225 230 AEREGDWSAMSGFQQTMQML lacI AEREGDWSAMSGFQQTMQML c g g a a c g g g a a g g c g a c t g g a g t g c c a t g t c c g g t t t t c a a c a a a c c a t g c a a a t g c t g a g c c t t g c c c t t c c g c t g a c c t c a c g g t a c a g g c c a a a a g t t g t t t g g t a c g t t t a c g a c t

840 235 240 245 250 NEGIVPTAMLVANDQMALGA lacI NEGIVPTAMLVANDQMALGA a t g a g g g c a t c g t t c c c a c t g c g a t g c t g g t t g c c a a c g a t c a g a t g g c g c t g g g c g c a a t a c t c c c g t a g c a a g g g t g a c g c t a c g a c c a a c g g t t g c t a g t c t a c c g c g a c c c g c g t t

900 255 260 265 270 MRAITESGLRVGADISVVGY lacI MRAITESGLRVGADISVVGY t g c g c g c c a t t a c c g a g t c c g g g c t g c g c g t t g g t g c g g a t a t t t c g g t a g t g g g a t a c g a c g c g c g g t a a t g g c t c a g g c c c g a c g c g c a a c c a c g c c t a t a a a g c c a t c a c c c t a t g c

960 275 280 285 290 DDTEDSSCYIPPLTTIKQDF lacI DDTEDSSCYIPPLTTIKQDF a c g a t a c c g a a g a c a g c t c a t g t t a t a t c c c g c c g t t a a c c a c c a t c a a a c a g g a t t t t c t g c t a t g g c t t c t g t c g a g t a c a a t a t a g g g c g g c a a t t g g t g g t a g t t t g t c c t a a a a g

1020 295 300 305 310 RLLGQTSVDRLLQLSQGQAV lacI RLLGQTSVDRLLQLSQGQAV g c c t g c t g g g g c a a a c c a g c g t g g a c c g c t t g c t g c a a c t c t c t c a g g g c c a g g c g g t g a c g g a c g a c c c c g t t t g g t c g c a c c t g g c g a a c g a c g t t g a g a g a g t c c c g g t c c g c c a c t

KasINarI*SfoIPluTI 1080 315 320 325 330 KGNQLLPVSLVKRKTTLAPN lacI KGNQLLPVSLVKRKTTLAPN a g g g c a a t c a g c t g t t g c c c g t c t c a c t g g t g a a a a g a a a a a c c a c c c t g g c g c c c a a t a t c c c g t t a g t c g a c a a c g g g c a g a g t g a c c a c t t t t c t t t t t g g t g g g a c c g c g g g t t a t

1140 335 340 345 350 TQTASPRALADSLMQLARQV lacI TQTASPRALADSLMQLARQV c a c a a a c c g c c t c t c c c c g c g c g t t g g c c g a t t c a t t a a t g c a g c t g g c a c g a c a g g t t t g t g t t t g g c g g a g a g g g g c g c g c a a c c g g c t a a g t a a t t a c g t c g a c c g t g c t g t c c a a a

1200 355 360 SRLESGQ* lacI SRLESGQ* c c c g a c t g g a a a g c g g g c a g t g a g c g c a a c g c a a t t a a t g t a a g t t a g c t c a c t c a t t a g g g g c t g a c c t t t c g c c c g t c a c t c g c g t t g c g t t a a t t a c a t t c a a t c g a g t g a g t a a t c

1260 g c a c a a t t c t c a t g t t t g a c a g c t t a t c a t c g a c t g c a c g g t g c a c c a a t g c t t c t g g c g c g t g t t a a g a g t a c a a a c t g t c g a a t a g t a g c t g a c g t g c c a c g t g g t t a c g a a g a c c g c

1320 t c a g g c a g c c a t c g g a a g c t g t g g t a t g g c t g t g c a g g t c g t a a a t c a c t g c a t a a t t c g a g t c c g t c g g t a g c c t t c g a c a c c a t a c c g a c a c g t c c a g c a t t t a g t g a c g t a t t a a g c

1380 t g t c g c t c a a g g c g c a c t c c c g t t c t g g a t a a t g t t t t t t g c g c c g a c a t c a t a a c g g t t a c a g c g a g t t c c g c g t g a g g g c a a g a c c t a t t a c a a a a a a c g c g g c t g t a g t a t t g c c a a

1440 -35 -10 tac promoter c t g g c a a a t a t t c t g a a a t g a g c t g t t g a c a a t t a a t c a t c g g c t c g t a t a a t g t g t g g a g a c c g t t t a t a a g a c t t t a c t c g a c a a c t g t t a a t t a g t a g c c g a g c a t a t t a c a c a c c t

1500 lac operator A G C G G A T A A C A A T T T C A C A C A G G M13/pUC Reverse a t t g t g a g c g g a t a a c a a t t t c a c a c a g g a a a c a g c c a g t c c g t t t a g g t g t t t t c a c g a t a a c a c t c g c c t a t t g t t a a a g t g t g t c c t t t g t c g g t c a g g c a a a t c c a c a a a a g t g c t

MfeI 1560 1 5 HHHHHH EE 6xHis MKIHHHHHHEE g c a a t t g a c c a a c a a g g a c c a t a g a t t a t g a a a a t c c a t c a c c a t c a c c a t c a c g a a g a a c g t t a a c t g g t t g t t c c t g g t a t c t a a t a c t t t t a g g t a g t g g t a g t g g t a g t g c t t c t t

1620 (in frame with 6xHis) GKLVIWINGDKGYNGLAEVG GKLVIWINGDKGYNGLAEVG g g t a a a c t g g t a a t c t g g a t t a a c g g c g a t a a a g g c t a t a a c g g t c t c g c t g a a g t c g g t c c a t t t g a c c a t t a g a c c t a a t t g c c g c t a t t t c c g a t a t t g c c a g a g c g a c t t c a g c c a

1680 (in frame with 6xHis) KKFEKDTGIKVTVEHPDKLE KKFEKDTGIKVTVEHPDKLE a a g a a a t t c g a g a a a g a t a c c g g a a t t a a a g t c a c c g t t g a g c a t c c g g a t a a a c t g g a a t t c t t t a a g c t c t t t c t a t g g c c t t a a t t t c a g t g g c a a c t c g t a g g c c t a t t t g a c c t t

1740 (in frame with 6xHis) EKFPQVAATGDGPDIIFWAH EKFPQVAATGDGPDIIFWAH g a g a a a t t c c c a c a g g t t g c g g c a a c t g g c g a t g g c c c t g a c a t t a t c t t c t g g g c a c a c c t c t t t a a g g g t g t c c a a c g c c g t t g a c c g c t a c c g g g a c t g t a a t a g a a g a c c c g t g t g

1800 (in frame with 6xHis) DRFGGYAQSGLLAEITPDKA DRFGGYAQSGLLAEITPDKA g a c c g c t t t g g t g g c t a c g c t c a a t c t g g c c t g t t g g c t g a a a t c a c c c c g g a c a a a g c g c t g g c g a a a c c a c c g a t g c g a g t t a g a c c g g a c a a c c g a c t t t a g t g g g g c c t g t t t c g c

BsiWI 1860 (in frame with 6xHis) FQDKLYPFTWDAVRYNGKLI FQDKLYPFTWDAVRYNGKLI t t c c a g g a c a a g c t g t a t c c g t t t a c c t g g g a t g c c g t a c g t t a c a a c g g c a a g c t g a t t a a g g t c c t g t t c g a c a t a g g c a a a t g g a c c c t a c g g c a t g c a a t g t t g c c g t t c g a c t a a

PsiI 1920 (in frame with 6xHis) AYPIAVEALSLIYNKDLLPN AYPIAVEALSLIYNKDLLPN g c t t a c c c g a t c g c t g t t g a a g c g t t a t c g c t g a t t t a t a a c a a a g a t c t g c t g c c g a a c c g a a t g g g c t a g c g a c a a c t t c g c a a t a g c g a c t a a a t a t t g t t t c t a g a c g a c g g c t t g

1980 (in frame with 6xHis) PPKTWEEIPALDKELKAKGK PPKTWEEIPALDKELKAKGK c c g c c a a a a a c c t g g g a a g a g a t c c c g g c g c t g g a t a a a g a a c t g a a a g c g a a a g g t a a g g g c g g t t t t t g g a c c c t t c t c t a g g g c c g c g a c c t a t t t c t t g a c t t t c g c t t t c c a t t c

2040 (in frame with 6xHis) SALMFNLQEPYFTWPLIAAD SALMFNLQEPYFTWPLIAAD a g c g c g c t g a t g t t c a a c c t g c a a g a a c c g t a c t t c a c c t g g c c g c t g a t t g c t g c t g a c t c g c g c g a c t a c a a g t t g g a c g t t c t t g g c a t g a a g t g g a c c g g c g a c t a a c g a c g a c t g

BmgBI 2100 (in frame with 6xHis) GGYAFKYENGKYDIKDVGVD GGYAFKYENGKYDIKDVGVD g g g g g t t a t g c g t t c a a g t a t g a a a a c g g c a a g t a c g a c a t t a a a g a c g t g g g c g t g g a t c c c c c a a t a c g c a a g t t c a t a c t t t t g c c g t t c a t g c t g t a a t t t c t g c a c c c g c a c c t a

2160 (in frame with 6xHis) NAGAKAGLTFLVDLIKNKHM NAGAKAGLTFLVDLIKNKHM a a c g c t g g c g c g a a a g c g g g t c t g a c c t t c c t g g t t g a c c t g a t t a a a a a c a a a c a c a t g t t g c g a c c g c g c t t t c g c c c a g a c t g g a a g g a c c a a c t g g a c t a a t t t t t g t t t g t g t a c

BsmI 2220 (in frame with 6xHis) NADTDYSIAEAAFNKGETAM NADTDYSIAEAAFNKGETAM a a t g c a g a c a c c g a t t a c t c c a t c g c a g a a g c t g c c t t t a a t a a a g g c g a a a c a g c g a t g t t a c g t c t g t g g c t a a t g a g g t a g c g t c t t c g a c g g a a a t t a t t t c c g c t t t g t c g c t a c

2280 (in frame with 6xHis) TINGPWAWSNIDTSKVNYGV TINGPWAWSNIDTSKVNYGV a c c a t c a a c g g c c c g t g g g c a t g g t c c a a c a t c g a c a c c a g c a a a g t g a a t t a t g g t g t a t g g t a g t t g c c g g g c a c c c g t a c c a g g t t g t a g c t g t g g t c g t t t c a c t t a a t a c c a c a t

BlpI 2340 (in frame with 6xHis) TVLPTFKGQPSKPFVGVLSA TVLPTFKGQPSKPFVGVLSA a c g g t a c t g c c g a c c t t c a a g g g t c a a c c a t c c a a a c c g t t c g t t g g c g t g c t g a g c g c a t g c c a t g a c g g c t g g a a g t t c c c a g t t g g t a g g t t t g g c a a g c a a c c g c a c g a c t c g c g t

2400 (in frame with 6xHis) GINAASPNKELAKEFLENYL GINAASPNKELAKEFLENYL g g t a t t a a c g c c g c c a g t c c g a a c a a a g a g c t g g c a a a a g a g t t c c t c g a a a a c t a t c t g c c a t a a t t g c g g c g g t c a g g c t t g t t t c t c g a c c g t t t t c t c a a g g a g c t t t t g a t a g a c

2460 (in frame with 6xHis) LTDEGLEAVNKDKPLGAVAL LTDEGLEAVNKDKPLGAVAL c t g a c t g a t g a a g g t c t g g a a g c g g t t a a t a a a g a c a a a c c g c t g g g t g c c g t a g c g c t g g a c t g a c t a c t t c c a g a c c t t c g c c a a t t a t t t c t g t t t g g c g a c c c a c g g c a t c g c g a c

2520 (in frame with 6xHis) KSYEEELVKDPRIAATMENA KSYEEELVKDPRIAATMENA a a g t c t t a c g a g g a a g a g t t g g t g a a a g a t c c g c g t a t t g c c g c c a c t a t g g a a a a c g c c t t c a g a a t g c t c c t t c t c a a c c a c t t t c t a g g c g c a t a a c g g c g g t g a t a c c t t t t g c g g

2580 (in frame with 6xHis) QKGEIMPNIPQMSAFWYAVR QKGEIMPNIPQMSAFWYAVR c a g a a a g g t g a a a t c a t g c c g a a c a t c c c g c a g a t g t c c g c t t t c t g g t a t g c c g t g c g t g t c t t t c c a c t t t a g t a c g g c t t g t a g g g c g t c t a c a g g c g a a a g a c c a t a c g g c a c g c a

2640 (in frame with 6xHis) TAVINAASGRQTVDEALKDA G A T G A A G C C C T G A A A G A C G C G MBP-F TAVINAASGRQTVDEALKDA a c t g c g g t g a t c a a c g c c g c c a g c g g t c g t c a g a c t g t c g a t g a a g c c c t g a a a g a c g c g t g a c g c c a c t a g t t g c g g c g g t c g c c a g c a g t c t g a c a g c t a c t t c g g g a c t t t c t g c g c

Eco53kISacI 2700 (in frame with 6xHis) QTNSSS 1 5 ENLYFQS A 5 QIFVCT TEV site ubiquitin C A G MBP-F QTNSSSENLYFQSAQIFVCT c a g a c t a a t t c g a g c t c t g a a a a c c t g t a t t t t c a g t c c g c t c a g a t c t t c g t c t g c a c g g t c t g a t t a a g c t c g a g a c t t t t g g a c a t a a a a g t c a g g c g a g t c t a g a a g c a g a c g t g c

AgeI 2760 10 15 20 25 LTGKTITLEVEPSDTIENVK ubiquitin LTGKTITLEVEPSDTIENVK t t a a c c g g t a a a a c c a t a a c t c t g g a a g t t g a a c c a t c c g a t a c c a t c g a a a a c g t t a a g a a t t g g c c a t t t t g g t a t t g a g a c c t t c a a c t t g g t a g g c t a t g g t a g c t t t t g c a a t t c

XmnI 2820 30 35 40 45 AKIQDKEGIPPDQQRLIFAG ubiquitin AKIQDKEGIPPDQQRLIFAG g c t a a a a t t c a a g a c a a g g a a g g a a t t c c a c c t g a t c a a c a a a g a t t g a t c t t t g c c g g t c g a t t t t a a g t t c t g t t c c t t c c t t a a g g t g g a c t a g t t g t t t c t a a c t a g a a a c g g c c a

SalIAccIPaeR7IBsoBIPspXIXhoIAvaI 2880 50 55 60 65 KQLEDGRTLSDYNIQKESTL ubiquitin KQLEDGRTLSDYNIQKESTL a a g c a g c t c g a g g a c g g t a g a a c g c t g t c t g a t t a c a a c a t t c a g a a g g a g t c g a c c t t a t t c g t c g a g c t c c t g c c a t c t t g c g a c a g a c t a a t g t t g t a a g t c t t c c t c a g c t g g a a t

SbfIPstIBamHIAflII 2940 70 75 HLVLRLRG C* ubiquitin HLVLRLRGC* c a t c t t g t c t t a a g a c t a a g a g g t t g c t a a g g a t c c g a a t t c c c t g c a g g t a a t t a a a t a g t a g a a c a g a a t t c t g a t t c t c c a a c g a t t c c t a g g c t t a a g g g a c g t c c a t t a a t t t a t

HindIII 3000 rrnB T1 terminator a g c t t c a a a t a a a a c g a a a g g c t c a g t c g a a a g a c t g g g c c t t t c g t t t t a t c t g t t g t t t c g a a g t t t a t t t t g c t t t c c g a g t c a g c t t t c t g a c c c g g a a a g c a a a a t a g a c a a c a a

3060 rrnB T1 terminator t g t c g g t g a a c g c t c t c c t g a g t a g g a c a a a t c c g c c g g g a g c g g a t t t g a a c g t t g c g a a c a g c c a c t t g c g a g a g g a c t c a t c c t g t t t a g g c g g c c c t c g c c t a a a c t t g c a a c g c t

3120 a g c a a c g g c c c g g a g g g t g g c g g g c a g g a c g c c c g c c a t a a a c t g c c a g g c a t c a a a t t a t c g t t g c c g g g c c t c c c a c c g c c c g t c c t g c g g g c g g t a t t t g a c g g t c c g t a g t t t a a t

RsrII 3180 rrnB T2 terminator AmpR promoter a g c a g a a g g c c a t c c t g a c g g a t g g c c t t t t t g c g t t t c t a c a a a c t c t t t c g g t c c g t t t c g t c t t c c g g t a g g a c t g c c t a c c g g a a a a a c g c a a a g a t g t t t g a g a a a g c c a g g c a a

BspHI 3240 AmpR promoter g t t t a t t t t t c t a a a t a c a t t c a a a t a t g t a t c c g c t c a t g a g a c a a t a a c c c t g a t a a a c a a a t a a a a a g a t t t a t g t a a g t t t a t a c a t a g g c g a g t a c t c t g t t a t t g g g a c t a t t t

3300 1 5 10 signal sequence MSIQHFRVAL AmpR promoter AmpR MSIQHFRVAL t g c t t c a a t a a t a t t g a a a a a g g a a g a g t a t g a g t a t t c a a c a t t t c c g t g t c g c c c t t a a c g a a g t t a t t a t a a c t t t t t c c t t c t c a t a c t c a t a a g t t g t a a a g g c a c a g c g g g a a t

3360 15 20 signal sequence IPFFAAFCLPVFA 25 30 HPETLVK AmpR IPFFAAFCLPVFAHPETLVK t t c c c t t t t t t g c g g c a t t t t g c c t t c c t g t t t t t g c t c a c c c a g a a a c g c t g g t g a a a g a a g g g a a a a a a c g c c g t a a a a c g g a a g g a c a a a a a c g a g t g g g t c t t t g c g a c c a c t t t c

3420 35 40 45 50 VKDAEDQLGARVGYIELDLN AmpR VKDAEDQLGARVGYIELDLN t a a a a g a t g c t g a a g a t c a g t t g g g t g c a c g a g t g g g t t a c a t c g a a c t g g a t c t c a a c a a t t t t c t a c g a c t t c t a g t c a a c c c a c g t g c t c a c c c a a t g t a g c t t g a c c t a g a g t t g t

DraI 3480 55 60 65 70 SGKILESFRPEERFPMMSTF AmpR SGKILESFRPEERFPMMSTF g c g g t a a g a t c c t t g a g a g t t t t c g c c c c g a a g a a c g t t t c c c a a t g a t g a g c a c t t t t a c g c c a t t c t a g g a a c t c t c a a a a g c g g g g c t t c t t g c a a a g g g t t a c t a c t c g t g a a a a t

3540 75 80 85 90 KVLLCGAVLSRVDAWQEQLG AmpR C G A T A C A C C G C G C C A T A A T A Amp-R KVLLCGAVLSRVDAWQEQLG a a g t t c t g c t a t g t g g c g c g g t a t t a t c c c g t g t t g a c g c c t g g c a a g a g c a a c t c g g t c t t c a a g a c g a t a c a c c g c g c c a t a a t a g g g c a c a a c t g c g g a c c g t t c t c g t t g a g c c a g

TatIScaI 3600 95 100 105 110 RRIHYSQNDLVEYSPVTEKH AmpR RRIHYSQNDLVEYSPVTEKH g c c g c a t a c a c t a t t c t c a g a a t g a c t t g g t t g a g t a c t c a c c a g t c a c a g a a a a g c a t c c g g c g t a t g t g a t a a g a g t c t t a c t g a a c c a a c t c a t g a g t g g t c a g t g t c t t t t c g t a g

3660 115 120 125 130 LTDGMTVRELCSAAITMSDN AmpR LTDGMTVRELCSAAITMSDN t t a c g g a t g g c a t g a c a g t a a g a g a a t t a t g c a g t g c t g c c a t a a c c a t g a g t g a t a a c a a a t g c c t a c c g t a c t g t c a t t c t c t t a a t a c g t c a c g a c g g t a t t g g t a c t c a c t a t t g t

3720 135 140 145 150 TAANLLLTTIGGPKELTAFL AmpR TAANLLLTTIGGPKELTAFL c t g c g g c c a a c t t a c t t c t g a c a a c g a t c g g a g g a c c g a a g g a g c t a a c c g c t t t t t t g c g a c g c c g g t t g a a t g a a g a c t g t t g c t a g c c t c c t g g c t t c c t c g a t t g g c g a a a a a a c g

3780 155 160 165 170 HNMGDHVTRLDRWEPELNEA AmpR HNMGDHVTRLDRWEPELNEA a c a a c a t g g g g g a t c a t g t a a c t c g c c t t g a t c g t t g g g a a c c g g a g c t g a a t g a a g c c a t g t t g t a c c c c c t a g t a c a t t g a g c g g a a c t a g c a a c c c t t g g c c t c g a c t t a c t t c g g t

3840 175 180 185 190 IPNDERDTTMPVAMATTLRK AmpR IPNDERDTTMPVAMATTLRK t a c c a a a c g a c g a g c g t g a c a c c a c g a t g c c t g t a g c a a t g g c a a c a a c g t t g c g c a a a c a t g g t t t g c t g c t c g c a c t g t g g t g c t a c g g a c a t c g t t a c c g t t g t t g c a a c g c g t t t g

3900 195 200 205 210 LLTGELLTLASRQQLIDWME AmpR LLTGELLTLASRQQLIDWME t a t t a a c t g g c g a a c t a c t t a c t c t a g c t t c c c g g c a a c a a t t a a t a g a c t g g a t g g a g g a t a a t t g a c c g c t t g a t g a a t g a g a t c g a a g g g c c g t t g t t a a t t a t c t g a c c t a c c t c c

BglI 3960 215 220 225 230 ADKVAGPLLRSALPAGWFIA AmpR ADKVAGPLLRSALPAGWFIA c g g a t a a a g t t g c a g g a c c a c t t c t g c g c t c g g c c c t t c c g g c t g g c t g g t t t a t t g c t g g c c t a t t t c a a c g t c c t g g t g a a g a c g c g a g c c g g g a a g g c c g a c c g a c c a a a t a a c g a c

4020 235 240 245 250 DKSGAGERGSRGIIAALGPD AmpR DKSGAGERGSRGIIAALGPD a t a a a t c t g g a g c c g g t g a g c g t g g g t c t c g c g g t a t c a t t g c a g c a c t g g g g c c a g a t g t a t t t a g a c c t c g g c c a c t c g c a c c c a g a g c g c c a t a g t a a c g t c g t g a c c c c g g t c t a c

4080 255 260 265 270 GKPSRIVVIYTTGSQATMDE AmpR GKPSRIVVIYTTGSQATMDE g t a a g c c c t c c c g t a t c g t a g t t a t c t a c a c g a c g g g g a g t c a g g c a a c t a t g g a t g a a c c a t t c g g g a g g g c a t a g c a t c a a t a g a t g t g c t g c c c c t c a g t c c g t t g a t a c c t a c t t g

4140 275 280 285 RNRQIAEIGASLIKHW* AmpR RNRQIAEIGASLIKHW* g a a a t a g a c a g a t c g c t g a g a t a g g t g c c t c a c t g a t t a a g c a t t g g t a a c t g t c a g a c c c t t t a t c t g t c t a g c g a c t c t a t c c a c g g a g t g a c t a a t t c g t a a c c a t t g a c a g t c t g g

Bsu36I 4200 a a g t t t a c t c a t a t a t a c t t t a g a t t g a t t t c c t t a g g a c t g a g c g t c a a c c c c g t a g a a t t c a a a t g a g t a t a t a t g a a a t c t a a c t a a a g g a a t c c t g a c t c g c a g t t g g g g c a t c t t

4260 ori a a g a t c a a a g g a t c t t c t t g a g a t c c t t t t t t t c t g c g c g t a a t c t g c t g c t t g c a a a c a t t c t a g t t t c c t a g a a g a a c t c t a g g a a a a a a a g a c g c g c a t t a g a c g a c g a a c g t t t g t

4320 ori a a a a a a c c a c c g c t a c c a g c g g t g g t t t g t t t g c c g g a t c a a g a g c t a c c a a c t c t t t t t t t t t t t g g t g g c g a t g g t c g c c a c c a a a c a a a c g g c c t a g t t c t c g a t g g t t g a g a a a a a

4380 ori c c g a a g g t a a c t g g c t t c a g c a g a g c g c a g a t a c c a a a t a c t g t c c t t c t a g t g t a g c c g g g c t t c c a t t g a c c g a a g t c g t c t c g c g t c t a t g g t t t a t g a c a g g a a g a t c a c a t c g g c

4440 ori t a g t t a g g c c a c c a c t t c a a g a a c t c t g t a g c a c c g c c t a c a t a c c t c g c t c t g c t a a t c a t c a a t c c g g t g g t g a a g t t c t t g a g a c a t c g t g g c g g a t g t a t g g a g c g a g a c g a t t a g

4500 ori c t g t t a c c a g t g g c t g c t g c c a g t g g c g a t a a g t c g t g t c t t a c c g g g t t g g a c t c a a g a g a c a a t g g t c a c c g a c g a c g g t c a c c g c t a t t c a g c a c a g a a t g g c c c a a c c t g a g t t c t

4560 ori c g a t a g t t a c c g g a t a a g g c g c a g c g g t c g g g c t g a a c g g g g g g t t c g t g c a c a c a g c c c g c t a t c a a t g g c c t a t t c c g c g t c g c c a g c c c g a c t t g c c c c c c a a g c a c g t g t g t c g g g

4620 ori a g c t t g g a g c g a a c g a c c t a c a c c g a a c t g a g a t a c c t a c a g c g t g a g c t a t g a g a a a g c t c g a a c c t c g c t t g c t g g a t g t g g c t t g a c t c t a t g g a t g t c g c a c t c g a t a c t c t t t c g

4680 ori g c c a c g c t t c c c g a a g g g a g a a a g g c g g a c a g g t a t c c g g t a a g c g g c a g g g t c g g a a c a c g g t g c g a a g g g c t t c c c t c t t t c c g c c t g t c c a t a g g c c a t t c g c c g t c c c a g c c t t g t

4740 ori G G G A A A C G C C T G G T A T C T T T pBR322ori-F g g a g a g c g c a c g a g g g a g c t t c c a g g g g g a a a c g c c t g g t a t c t t t a t a g t c c t g t c g g g c c t c t c g c g t g c t c c c t c g a a g g t c c c c c t t t g c g g a c c a t a g a a a t a t c a g g a c a g c c c

4800 ori t t t c g c c a c c t c t g a c t t g a g c g t c g a t t t t t g t g a t g c t c g t c a g g g g g g c g g a g c c t a a a a g c g g t g g a g a c t g a a c t c g c a g c t a a a a a c a c t a c g a g c a g t c c c c c c g c c t c g g a t

4860 ori t g g a a a a a c g c c a g c a a c g c g g c c t t t t t a c g g t t c c t g g c c t t t t g c t g g c c t t t t g c t a c c t t t t t g c g g t c g t t g c g c c g g a a a a a t g c c a a g g a c c g g a a a a c g a c c g g a a a a c g a

PciI 4920 c a c a t g t t c t t t c c t g c g t t a t c c c c t g a t t c t g t g g a t a a c c g t a t t a c c g c c t t t g a g g t g t a c a a g a a a g g a c g c a a t a g g g g a c t a a g a c a c c t a t t g g c a t a a t g g c g g a a a c t c

BspQISapI 4980 A G C G A G T C A G T G A G C G A G L4440 t g a g c t g a t a c c g c t c g c c g c a g c c g a a c g a c c g a g c g c a g c g a g t c a g t g a g c g a g g a a a c t c g a c t a t g g c g a g c g g c g t c g g c t t g c t g g c t c g c g t c g c t c a g t c a c t c g c t c c t t

5040 g c g g a a g a g c g c c t g a t g c g g t a t t t t c t c c t t a c g c a t c t g t g c g g t a t t t c a c a c c g c c g c c t t c t c g c g g a c t a c g c c a t a a a a g a g g a a t g c g t a g a c a c g c c a t a a a g t g t g g c g

Tth111IPflFI 5100 a t a t a a g g t g c a c t g t g a c t g g g t c a t g g c t g c g c c c c g a c a c c c g c c a a c a c c c g c t g a t a t a t t c c a c g t g a c a c t g a c c c a g t a c c g a c g c g g g g c t g t g g g c g g t t g t g g g c g a c t

5160 C pGEX 3' c g c g c c c t g a c g g g c t t g t c t g c t c c c g g c a t c c g c t t a c a g a c a a g c t g t g a c c g t c t c g c g c g g g a c t g c c c g a a c a g a c g a g g g c c g t a g g c g a a t g t c t g t t c g a c a c t g g c a g a g

5220 60 55 50 *LNEGDDGFRALCSR rop C G G G A G C T G C A T G T G T C A G A G G pGEX 3' c g g g a g c t g c a t g t g t c a g a g g t t t t c a c c g t c a t c a c c g a a a c g c g c g a g g c a g c t g c g g c c c t c g a c g t a c a c a g t c t c c a a a a g t g g c a g t a g t g g c t t t g c g c g c t c c g t c g a c g c

5280 45 40 35 30 YLEDAHDHLSECIDAQEDAD rop g t a a a g c t c a t c a g c g t g g t c g t g c a g c g a t t c a c a g a t g t c t g c c t g t t c a t c c g c g t c c a t t t c g a g t a g t c g c a c c a g c a c g t c g c t a a g t g t c t a c a g a c g g a c a a g t a g g c g c a g

5340 25 20 15 10 LENLKELLTLTQSRIFRAMN rop c a g c t c g t t g a g t t t c t c c a g a a g c g t t a a t g t c t g g c t t c t g a t a a a g c g g g c c a t g t t g t c g a g c a a c t c a a a g a g g t c t t c g c a a t t a c a g a c c g a a g a c t a t t t c g c c c g g t a c a a

5400 5 1 LATKEQKTM rop a a g g g c g g t t t t t t c c t g t t t g g t c a c t g a t g c c t c c g t g t a a g g g g g a t t t c t g t t c a t t t c c c g c c a a a a a a g g a c a a a c c a g t g a c t a c g g a g g c a c a t t c c c c c t a a a g a c a a g t a

5460 g g g g g t a a t g a t a c c g a t g a a a c g a g a g a g g a t g c t c a c g a t a c g g g t t a c t g a t g a t g a c c c c c a t t a c t a t g g c t a c t t t g c t c t c t c c t a c g a g t g c t a t g c c c a a t g a c t a c t a c t

5520 a c a t g c c c g g t t a c t g g a a c g t t g t g a g g g t a a a c a a c t g g c g g t a t g g a t g c g g c g g g a t g t a c g g g c c a a t g a c c t t g c a a c a c t c c c a t t t g t t g a c c g c c a t a c c t a c g c c g c c c t

5580 c c a g a g a a a a a t c a c t c a g g g t c a a t g c c a g c g c t t c g t t a a t a c a g a t g t a g g t g t t c c g g t c t c t t t t t a g t g a g t c c c a g t t a c g g t c g c g a a g c a a t t a t g t c t a c a t c c a c a a g g

5640 a c a g g g t a g c c a g c a g c a t c c t g c g a t g c a g a t c c g g a a c a t a a t g g t g c a g g g c g c t g a t g t c c c a t c g g t c g t c g t a g g a c g c t a c g t c t a g g c c t t g t a t t a c c a c g t c c c g c g a c t

5700 c t t c c g c g t t t c c a g a c t t t a c g a a a c a c g g a a a c c g a a g a c c a t t c a t g t t g t t g c t c a g a a g g c g c a a a g g t c t g a a a t g c t t t g t g c c t t t g g c t t c t g g t a a g t a c a a c a a c g a g t

5760 g g t c g c a g a c g t t t t g c a g c a g c a g t c g c t t c a c g t t c g c t c g c g t a t c g g t g a t t c a t t c c a g c g t c t g c a a a a c g t c g t c g t c a g c g a a g t g c a a g c g a g c g c a t a g c c a c t a a g t a a

5820 c t g c t a a c c a g t a a g g c a a c c c c g c c a g c c t a g c c g g g t c c t c a a c g a c a g g a g c a c g a t g a c g a t t g g t c a t t c c g t t g g g g c g g t c g g a t c g g c c c a g g a g t t g c t g t c c t c g t g c t a

MscI*FspAI 3ʹ 5ʹ 5860 c a t g c g c a c c c g t g g c c a g g a c c c a a c g c t g c c c g a a a t t g t a c g c g t g g g c a c c g g t c c t g g g t t g c g a c g g g c t t t a a

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.