Addgene pET tdTomato LIC cloning vector (u-tdTomato) - Sequence Analyzer Skip to main content
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Sequence Analyzer: pET tdTomato LIC cloning vector (u-tdTomato)


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EcoRI (6190) tet promoter BspDI - ClaI (6166) HindIII (6159) BmtI (5963) NheI (5959) T7 Term (5728 .. 5746) BlpI (5731) MluI (5666) KpnI (5664) Acc65I (5660) FseI (5657) DraIII (5649) AscI (5638) AsiSI (5633) ApaI (5306) PspOMI (5302) BstEII (5217) NcoI (4857) KflI (4526) PsiI (4424) BstBI (4259) XbaI (4105) PaeR7I - PspXI - XhoI (4098) NotI (4082) PacI (4077) SacI (4070) Eco53kI (4068) T7 (4010 .. 4029) BglII (3990) SgrAI (3949) pBRrevBam (3907 .. 3926) SphI (3801) EcoNI (3736) SalI (3708) PshAI (3647) NruI (3389) BstAPI (3315) BfuAI - BspMI (3305) pBRforEco (18 .. 36) ZraI (75) AatII (77) Amp-R (427 .. 446) ScaI (515) AhdI (996) pBR322ori-F (1729 .. 1748) PciI (1884) L4440 (1982 .. 1999) BspQI - SapI (2001) pRS-marker (2082 .. 2101) BstZ17I (2117) PflFI - Tth111I (2142) PfoI (2241) pGEX 3' (2242 .. 2264) BsmI (3006) pET tdTomato LIC cloning vector (u-tdTomato) 6192 bp

Sequence View

5ʹ 3ʹ 60 C G G A G C A C T A T G C G G A T A A pBRforEco t t c t t g a a g a c g a a a g g g c c t c g t g a t a c g c c t a t t t t t a t a g g t t a a t g t c a t g a t a a t a a g a a c t t c t g c t t t c c c g g a g c a c t a t g c g g a t a a a a a t a t c c a a t t a c a g t a c t a t t a

ZraIAatII 120 AmpR promoter a a t g g t t t c t t a g a c g t c a g g t g g c a c t t t t c g g g g a a a t g t g c g c g g a a c c c c t a t t t g t t a c c a a a g a a t c t g c a g t c c a c c g t g a a a a g c c c c t t t a c a c g c g c c t t g g g g a t a a a c

180 AmpR promoter t t t a t t t t t c t a a a t a c a t t c a a a t a t g t a t c c g c t c a t g a g a c a a t a a c c c t g a t a a a t a a a t a a a a a g a t t t a t g t a a g t t t a t a c a t a g g c g a g t a c t c t g t t a t t g g g a c t a t t t a

240 1 5 10 signal sequence MSIQHFRVALI AmpR promoter AmpR MSIQHFRVALI g c t t c a a t a a c a t t g a a a a a g g a a g a g t a t g a g t a t t c a a c a t t t c c g t g t c g c c c t t a t c g a a g t t a t t g t a a c t t t t t c c t t c t c a t a c t c a t a a g t t g t a a a g g c a c a g c g g g a a t a

300 15 20 signal sequence PFFAAFCLPVFA 25 30 HPETLVKV AmpR PFFAAFCLPVFAHPETLVKV t c c c t t t t t t g c g g c a t t t t g c c t t c c t g t t t t t g c t c a c c c a g a a a c g c t g g t g a a a g t a g g g a a a a a a c g c c g t a a a a c g g a a g g a c a a a a a c g a g t g g g t c t t t g c g a c c a c t t t c a

360 35 40 45 50 KDAEDQLGARVGYIELDLNS AmpR KDAEDQLGARVGYIELDLNS a a a a g a t g c t g a a g a t c a g t t g g g t g c a c g a g t g g g t t a c a t c g a a c t g g a t c t c a a c a g t t t t c t a c g a c t t c t a g t c a a c c c a c g t g c t c a c c c a a t g t a g c t t g a c c t a g a g t t g t c

420 55 60 65 70 GKILESFRPEERFPMMSTFK AmpR GKILESFRPEERFPMMSTFK c g g t a a g a t c c t t g a g a g t t t t c g c c c c g a a g a a c g t t t t c c a a t g a t g a g c a c t t t t a a g c c a t t c t a g g a a c t c t c a a a a g c g g g g c t t c t t g c a a a a g g t t a c t a c t c g t g a a a a t t

480 75 80 85 90 VLLCGAVLSRVDAGQEQLGR AmpR C G A T A C A C C G C G C C A T A A T A Amp-R VLLCGAVLSRVDAGQEQLGR a g t t c t g c t a t g t g g c g c g g t a t t a t c c c g t g t t g a c g c c g g g c a a g a g c a a c t c g g t c g t c a a g a c g a t a c a c c g c g c c a t a a t a g g g c a c a a c t g c g g c c c g t t c t c g t t g a g c c a g c

ScaI 540 95 100 105 110 RIHYSQNDLVEYSPVTEKHL AmpR RIHYSQNDLVEYSPVTEKHL c c g c a t a c a c t a t t c t c a g a a t g a c t t g g t t g a g t a c t c a c c a g t c a c a g a a a a g c a t c t g g c g t a t g t g a t a a g a g t c t t a c t g a a c c a a c t c a t g a g t g g t c a g t g t c t t t t c g t a g a

600 115 120 125 130 TDGMTVRELCSAAITMSDNT AmpR TDGMTVRELCSAAITMSDNT t a c g g a t g g c a t g a c a g t a a g a g a a t t a t g c a g t g c t g c c a t a a c c a t g a g t g a t a a c a c a t g c c t a c c g t a c t g t c a t t c t c t t a a t a c g t c a c g a c g g t a t t g g t a c t c a c t a t t g t g

660 135 140 145 150 AANLLLTTIGGPKELTAFLH AmpR AANLLLTTIGGPKELTAFLH t g c g g c c a a c t t a c t t c t g a c a a c g a t c g g a g g a c c g a a g g a g c t a a c c g c t t t t t t g c a a c g c c g g t t g a a t g a a g a c t g t t g c t a g c c t c c t g g c t t c c t c g a t t g g c g a a a a a a c g t

720 155 160 165 170 NMGDHVTRLDRWEPELNEAI AmpR NMGDHVTRLDRWEPELNEAI c a a c a t g g g g g a t c a t g t a a c t c g c c t t g a t c g t t g g g a a c c g g a g c t g a a t g a a g c c a t g t t g t a c c c c c t a g t a c a t t g a g c g g a a c t a g c a a c c c t t g g c c t c g a c t t a c t t c g g t a

780 175 180 185 190 PNDERDTTMPAAMATTLRKL AmpR PNDERDTTMPAAMATTLRKL a c c a a a c g a c g a g c g t g a c a c c a c g a t g c c t g c a g c a a t g g c a a c a a c g t t g c g c a a a c t t g g t t t g c t g c t c g c a c t g t g g t g c t a c g g a c g t c g t t a c c g t t g t t g c a a c g c g t t t g a

840 195 200 205 210 LTGELLTLASRQQLIDWMEA AmpR LTGELLTLASRQQLIDWMEA a t t a a c t g g c g a a c t a c t t a c t c t a g c t t c c c g g c a a c a a t t a a t a g a c t g g a t g g a g g c t a a t t g a c c g c t t g a t g a a t g a g a t c g a a g g g c c g t t g t t a a t t a t c t g a c c t a c c t c c g

900 215 220 225 230 DKVAGPLLRSALPAGWFIAD AmpR DKVAGPLLRSALPAGWFIAD g g a t a a a g t t g c a g g a c c a c t t c t g c g c t c g g c c c t t c c g g c t g g c t g g t t t a t t g c t g a c c t a t t t c a a c g t c c t g g t g a a g a c g c g a g c c g g g a a g g c c g a c c g a c c a a a t a a c g a c t

960 235 240 245 250 KSGAGERGSRGIIAALGPDG AmpR KSGAGERGSRGIIAALGPDG t a a a t c t g g a g c c g g t g a g c g t g g g t c t c g c g g t a t c a t t g c a g c a c t g g g g c c a g a t g g a t t t a g a c c t c g g c c a c t c g c a c c c a g a g c g c c a t a g t a a c g t c g t g a c c c c g g t c t a c c

AhdI 1020 255 260 265 270 KPSRIVVIYTTGSQATMDER AmpR KPSRIVVIYTTGSQATMDER t a a g c c c t c c c g t a t c g t a g t t a t c t a c a c g a c g g g g a g t c a g g c a a c t a t g g a t g a a c g a t t c g g g a g g g c a t a g c a t c a a t a g a t g t g c t g c c c c t c a g t c c g t t g a t a c c t a c t t g c

1080 275 280 285 NRQIAEIGASLIKHW* AmpR NRQIAEIGASLIKHW* a a a t a g a c a g a t c g c t g a g a t a g g t g c c t c a c t g a t t a a g c a t t g g t a a c t g t c a g a c c a t t t a t c t g t c t a g c g a c t c t a t c c a c g g a g t g a c t a a t t c g t a a c c a t t g a c a g t c t g g t

1140 a g t t t a c t c a t a t a t a c t t t a g a t t g a t t t a a a a c t t c a t t t t t a a t t t a a a a g g a t c t a t c a a a t g a g t a t a t a t g a a a t c t a a c t a a a t t t t g a a g t a a a a a t t a a a t t t t c c t a g a t

1200 g g t g a a g a t c c t t t t t g a t a a t c t c a t g a c c a a a a t c c c t t a a c g t g a g t t t t c g t t c c a c c a c t t c t a g g a a a a a c t a t t a g a g t a c t g g t t t t a g g g a a t t g c a c t c a a a a g c a a g g t

1260 ori c t g a g c g t c a g a c c c c g t a g a a a a g a t c a a a g g a t c t t c t t g a g a t c c t t t t t t t c t g c g g a c t c g c a g t c t g g g g c a t c t t t t c t a g t t t c c t a g a a g a a c t c t a g g a a a a a a a g a c g c

1320 ori c g t a a t c t g c t g c t t g c a a a c a a a a a a a c c a c c g c t a c c a g c g g t g g t t t g t t t g c c g g a g c a t t a g a c g a c g a a c g t t t g t t t t t t t g g t g g c g a t g g t c g c c a c c a a a c a a a c g g c c t

1380 ori t c a a g a g c t a c c a a c t c t t t t t c c g a a g g t a a c t g g c t t c a g c a g a g c g c a g a t a c c a a a a g t t c t c g a t g g t t g a g a a a a a g g c t t c c a t t g a c c g a a g t c g t c t c g c g t c t a t g g t t t

1440 ori t a c t g t c c t t c t a g t g t a g c c g t a g t t a g g c c a c c a c t t c a a g a a c t c t g t a g c a c c g c c a t g a c a g g a a g a t c a c a t c g g c a t c a a t c c g g t g g t g a a g t t c t t g a g a c a t c g t g g c g g

1500 ori t a c a t a c c t c g c t c t g c t a a t c c t g t t a c c a g t g g c t g c t g c c a g t g g c g a t a a g t c g t g a t g t a t g g a g c g a g a c g a t t a g g a c a a t g g t c a c c g a c g a c g g t c a c c g c t a t t c a g c a c

1560 ori t c t t a c c g g g t t g g a c t c a a g a c g a t a g t t a c c g g a t a a g g c g c a g c g g t c g g g c t g a a c a g a a t g g c c c a a c c t g a g t t c t g c t a t c a a t g g c c t a t t c c g c g t c g c c a g c c c g a c t t g

1620 ori g g g g g g t t c g t g c a c a c a g c c c a g c t t g g a g c g a a c g a c c t a c a c c g a a c t g a g a t a c c t c c c c c c a a g c a c g t g t g t c g g g t c g a a c c t c g c t t g c t g g a t g t g g c t t g a c t c t a t g g a

1680 ori a c a g c g t g a g c t a t g a g a a a g c g c c a c g c t t c c c g a a g g g a g a a a g g c g g a c a g g t a t c c t g t c g c a c t c g a t a c t c t t t c g c g g t g c g a a g g g c t t c c c t c t t t c c g c c t g t c c a t a g g

1740 ori G G G A A A C G C C T G pBR322ori-F g g t a a g c g g c a g g g t c g g a a c a g g a g a g c g c a c g a g g g a g c t t c c a g g g g g a a a c g c c t g c c a t t c g c c g t c c c a g c c t t g t c c t c t c g c g t g c t c c c t c g a a g g t c c c c c t t t g c g g a c

1800 ori G T A T C T T T pBR322ori-F g t a t c t t t a t a g t c c t g t c g g g t t t c g c c a c c t c t g a c t t g a g c g t c g a t t t t t g t g a t g c a t a g a a a t a t c a g g a c a g c c c a a a g c g g t g g a g a c t g a a c t c g c a g c t a a a a a c a c t a c

1860 ori c t c g t c a g g g g g g c g g a g c c t a t g g a a a a a c g c c a g c a a c g c g g c c t t t t t a c g g t t c c t g a g c a g t c c c c c c g c c t c g g a t a c c t t t t t g c g g t c g t t g c g c c g g a a a a a t g c c a a g g a

PciI 1920 g g c c t t t t g c t g g c c t t t t g c t c a c a t g t t c t t t c c t g c g t t a t c c c c t g a t t c t g t g g a c c g g a a a a c g a c c g g a a a a c g a g t g t a c a a g a a a g g a c g c a a t a g g g g a c t a a g a c a c c t

1980 t a a c c g t a t t a c c g c c t t t g a g t g a g c t g a t a c c g c t c g c c g c a g c c g a a c g a c c g a g c g a t t g g c a t a a t g g c g g a a a c t c a c t c g a c t a t g g c g a g c g g c g t c g g c t t g c t g g c t c g c

BspQISapI 2040 bom A G C G A G T C A G T G A G C G A G L4440 c a g c g a g t c a g t g a g c g a g g a a g c g g a a g a g c g c c t g a t g c g g t a t t t t c t c c t t a c g c a g t c g c t c a g t c a c t c g c t c c t t c g c c t t c t c g c g g a c t a c g c c a t a a a a g a g g a a t g c g t

2100 bom A T G T T A G A C G A G A C T A C G G pRS-marker t c t g t g c g g t a t t t c a c a c c g c a t a t a t g g t g c a c t c t c a g t a c a a t c t g c t c t g a t g c c a g a c a c g c c a t a a a g t g t g g c g t a t a t a c c a c g t g a g a g t c a t g t t a g a c g a g a c t a c g g

Tth111IPflFIBstZ17I 2160 bom C pRS-marker g c a t a g t t a a g c c a g t a t a c a c t c c g c t a t c g c t a c g t g a c t g g g t c a t g g c t g c g c c c c c g t a t c a a t t c g g t c a t a t g t g a g g c g a t a g c g a t g c a c t g a c c c a g t a c c g a c g c g g g g

2220 g a c a c c c g c c a a c a c c c g c t g a c g c g c c c t g a c g g g c t t g t c t g c t c c c g g c a t c c g c t t c t g t g g g c g g t t g t g g g c g a c t g c g c g g g a c t g c c c g a a c a g a c g a g g g c c g t a g g c g a a

PfoI 2280 60 *LNEGDDG rop C C G G G A G C T G C A T G T G T C A G A G G pGEX 3' a c a g a c a a g c t g t g a c c g t c t c c g g g a g c t g c a t g t g t c a g a g g t t t t c a c c g t c a t c a c t g t c t g t t c g a c a c t g g c a g a g g c c c t c g a c g t a c a c a g t c t c c a a a a g t g g c a g t a g t g

2340 55 50 45 40 FRALCSRYLEDAHDHLSECI rop c g a a a c g c g c g a g g c a g c t g c g g t a a a g c t c a t c a g c g t g g t c g t g a a g c g a t t c a c a g a g c t t t g c g c g c t c c g t c g a c g c c a t t t c g a g t a g t c g c a c c a g c a c t t c g c t a a g t g t c t

2400 35 30 25 20 DAQEDADLENLKELLTLTQS rop t g t c t g c c t g t t c a t c c g c g t c c a g c t c g t t g a g t t t c t c c a g a a g c g t t a a t g t c t g g c a c a g a c g g a c a a g t a g g c g c a g g t c g a g c a a c t c a a a g a g g t c t t c g c a a t t a c a g a c c g

2460 15 10 5 1 RIFRAMNLATKEQKTM rop t t c t g a t a a a g c g g g c c a t g t t a a g g g c g g t t t t t t c c t g t t t g g t c a c t g a t g c c t c c g a a g a c t a t t t c g c c c g g t a c a a t t c c c g c c a a a a a a g g a c a a a c c a g t g a c t a c g g a g g c

2520 t g t a a g g g g g a t t t c t g t t c a t g g g g g t a a t g a t a c c g a t g a a a c g a g a g a g g a t g c t c a a c a t t c c c c c t a a a g a c a a g t a c c c c c a t t a c t a t g g c t a c t t t g c t c t c t c c t a c g a g t

2580 c g a t a c g g g t t a c t g a t g a t g a a c a t g c c c g g t t a c t g g a a c g t t g t g a g g g t a a a c a a c g c t a t g c c c a a t g a c t a c t a c t t g t a c g g g c c a a t g a c c t t g c a a c a c t c c c a t t t g t t g

2640 t g g c g g t a t g g a t g c g g c g g g a c c a g a g a a a a a t c a c t c a g g g t c a a t g c c a g c g c t t c g a c c g c c a t a c c t a c g c c g c c c t g g t c t c t t t t t a g t g a g t c c c a g t t a c g g t c g c g a a g c

2700 t t a a t a c a g a t g t a g g t g t t c c a c a g g g t a g c c a g c a g c a t c c t g c g a t g c a g a t c c g g a a a t t a t g t c t a c a t c c a c a a g g t g t c c c a t c g g t c g t c g t a g g a c g c t a c g t c t a g g c c t

2760 a c a t a a t g g t g c a g g g c g c t g a c t t c c g c g t t t c c a g a c t t t a c g a a a c a c g g a a a c c g a t g t a t t a c c a c g t c c c g c g a c t g a a g g c g c a a a g g t c t g a a a t g c t t t g t g c c t t t g g c t

2820 a g a c c a t t c a t g t t g t t g c t c a g g t c g c a g a c g t t t t g c a g c a g c a g t c g c t t c a c g t t c t c t g g t a a g t a c a a c a a c g a g t c c a g c g t c t g c a a a a c g t c g t c g t c a g c g a a g t g c a a g

2880 g c t c g c g t a t c g g t g a t t c a t t c t g c t a a c c a g t a a g g c a a c c c c g c c a g c c t a g c c g g g c g a g c g c a t a g c c a c t a a g t a a g a c g a t t g g t c a t t c c g t t g g g g c g g t c g g a t c g g c c c

2940 t c c t c a a c g a c a g g a g c a c g a t c a t g c g c a c c c g t g g c c a g g a c c c a a c g c t g c c c g a g a a g g a g t t g c t g t c c t c g t g c t a g t a c g c g t g g g c a c c g g t c c t g g g t t g c g a c g g g c t c t

3000 t g c g c c g c g t g c g g c t g c t g g a g a t g g c g g a c g c g a t g g a t a t g t t c t g c c a a g g g t t g g a c g c g g c g c a c g c c g a c g a c c t c t a c c g c c t g c g c t a c c t a t a c a a g a c g g t t c c c a a c c

BsmI 3060 t t t g c g c a t t c a c a g t t c t c c g c a a g a a t t g a t t g g c t c c a a t t c t t g g a g t g g t g a a t c a a a c g c g t a a g t g t c a a g a g g c g t t c t t a a c t a a c c g a g g t t a a g a a c c t c a c c a c t t a g

3120 *TSTARSWAGR c g t t a g c g a g g t g c c g c c g g c t t c c a t t c a g g t c g a g g t g g c c c g g c t c c a t g c a c c g c g g c a a t c g c t c c a c g g c g g c c g a a g g t a a g t c c a g c t c c a c c g g g c c g a g g t a c g t g g c g c

3180 RLAPLCVLYLAAGVIWALGN a c g c a a c g c g g g g a g g c a g a c a a g g t a t a g g g c g g c g c c t a c a a t c c a t g c c a a c c c g t t t g c g t t g c g c c c c t c c g t c t g t t c c a t a t c c c g c c g c g g a t g t t a g g t a c g g t t g g g c a a

3240 WTSASAAYIATVILPGTIST c c a t g t g c t c g c c g a g g c g g c a t a a a t c g c c g t g a c g a t c a g c g g t c c a g t g a t c g a a g t g g t a c a c g a g c g g c t c c g c c g t a t t t a g c g g c a c t g c t a g t c g c c a g g t c a c t a g c t t c a

3300 MVVIYL LSTLAALSGQLQGQHDDDVQ t a g g c t g g t a a g a g c c g c g a g c g a t c c t t g a a g c t g t c c c t g a t g g t c g t c a t c t a c c t g a t c c g a c c a t t c t c g g c g c t c g c t a g g a a c t t c g a c a g g g a c t a c c a g c a g t a g a t g g a c

BstAPIBspMIBfuAI 3360 PGQHGLQRGHPDAAGSEKNH RSLMAQLAPMGIGGSALLIM c c t g g a c a g c a t g g c c t g c a a c g c g g g c a t c c c g a t g c c g c c g g a a g c g a g a a g a a t c a t g g a c c t g t c g t a c c g g a c g t t g c g c c c g t a g g g c t a c g g c g g c c t t c g c t c t t c t t a g t a

NruI 3420 NGEGHPASRRERQQDVAQRV IPFAMWGRTAFALLVYGLAD a a t g g g g a a g g c c a t c c a g c c t c g c g t c g c g a a c g c c a g c a a g a c g t a g c c c a g c g c g t c t t a c c c c t t c c g g t a g g t c g g a g c g c a g c g c t t g c g g t c g t t c t g c a t c g g g t c g c g c a g

3480 GRHAGDNGLLLAETFGGGTS AAMGAIIAQKEGFRKTAPGT g g c c g c c a t g c c g g c g a t a a t g g c c t g c t t c t c g c c g a a a c g t t t g g t g g c g g g a c c a g t c c g g c g g t a c g g c c g c t a t t a c c g g a c g a a g a g c g g c t t t g c a a a c c a c c g c c c t g g t c a

3540 DEGLSEGVQDSEYRKRQADH VFAQALAHLIGFVALSLGIM g a c g a a g g c t t g a g c g a g g g c g t g c a a g a t t c c g a a t a c c g c a a g c g a c a g g c c g a t c a t c t g c t t c c g a a c t c g c t c c c g c a c g t t c t a a g g c t t a t g g c g t t c g c t g t c c g g c t a g t a

3600 RRAPAKAVLAENDPERCRHL TASWRFRDEGFIVWLAAPVQ c g t c g c g c t c c a g c g a a a g c g g t c c t c g c c g a a a a t g a c c c a g a g c g c t g c c g g c a c c t g g c a g c g c g a g g t c g c t t t c g c c a g g a g c g g c t t t t a c t g g g t c t c g c g a c g g c c g t g g a c

PshAI 3660 SYELHDKEDSHKCGDDSHAP GVLQMIFFVTMLAAVITMGR t c c t a c g a g t t g c a t g a t a a a g a a g a c a g t c a t a a g t g c g g c g a c g a t a g t c a t g c c c c g a g g a t g c t c a a c g t a c t a t t t c t t c t g t c a g t a t t c a c g c c g c t g c t a t c a g t a c g g g g c

SalI 3720 RPPEGADWVEGSQGHRSTLS AWRFSSVPNFARLPMPRREG c g c c c a c c g g a a g g a g c t g a c t g g g t t g a a g g c t c t c a a g g g c a t c g g t c g a c g c t c t c c g c g g g t g g c c t t c c t c g a c t g a c c c a a c t t c c g a g a g t t c c c g t a g c c a g c t g c g a g a g g

EcoNI 3780 LMRLLH* KHSEQMLFCGLLLNLGNLVA c t t a t g c g a c t c c t g c a t t a g g a a g c a g c c c a g t a g t a g g t t g a g g c c g t t g a g c a c c g c g a a t a c g c t g a g g a c g t a a t c c t t c g t c g g g t c a t c a t c c a a c t c c g g c a a c t c g t g g c g

SphI 3840 AALFPAHLSIAGLLGGAVPG c g c c g c a a g g a a t g g t g c a t g c a a g g a g a t g g c g c c c a a c a g t c c c c c g g c c a c g g g g c c g c g g c g t t c c t t a c c a c g t a c g t t c c t c t a c c g c g g g t t g t c a g g g g g c c g g t g c c c c g g

3900 AVMGVGFCASM t g c c a c c a t a c c c a c g c c g a a a c a a g c g c t c a t g a g c c c g a a g t g g c g a g c c c g a t c t t c a c g g t g g t a t g g g t g c g g c t t t g t t c g c g a g t a c t c g g g c t t c a c c g c t c g g g c t a g a a g

SgrAI 3960 G G T G A T G T C G G C G A T A T A G G pBRrevBam c c c a t c g g t g a t g t c g g c g a t a t a g g c g c c a g c a a c c g c a c c t g t g g c g c c g g t g a t g c c g g g t a g c c a c t a c a g c c g c t a t a t c c g c g g t c g t t g g c g t g g a c a c c g c g g c c a c t a c g g

BglII 4020 T7 promoter T A A T A C G A C T C T7 g g c c a c g a t g c g t c c g g c g t a g a g g a t c g a g a t c t c g a t c c c g c g a a a t t a a t a c g a c t c c c g g t g c t a c g c a g g c c g c a t c t c c t a g c t c t a g a g c t a g g g c g c t t t a a t t a t g c t g a g

PacIEco53kISacI 4080 T7 promoter A C T A T A G G G T7 a c t a t a g g g a g a c c a c a a c g g t t t c c c t c t a g t g c c g g c t c c g g a g a g c t c t t t a a t t a a t g a t a t c c c t c t g g t g t t g c c a a a g g g a g a t c a c g g c c g a g g c c t c t c g a g a a a t t a a t t

XbaIPaeR7IPspXIXhoINotI 4140 RBS g c g g c c g c c c t g c a g g a c t c g a g t t c t a g a a a t a a t t t t g t t t a a c t t t a a g a a g g a g a t c g c c g g c g g g a c g t c c t g a g c t c a a g a t c t t t a t t a a a a c a a a t t g a a a t t c t t c c t c t a

4200 *GGVAAASGHTLALFH a t a g a t a t c c t c t c a t c c t c c a a c g g c a g c a g c g g a t c c g t g a g t a a g g g c g a g g a a g t g t a t c t a t a g g a g a g t a g g a g g t t g c c g t c g t c g c c t a g g c a c t c a t t c c c g c t c c t t c a c

BstBI 4260 MRFKVRMEGSMNGHEF DFLEHPKLHSHFSAHVAVLE a t c a a a g a g t t c a t g c g g t t t a a g g t g a g a a t g g a a g g a a g c a t g a a c g g c c a c g a g t t c t a g t t t c t c a a g t a c g c c a a a t t c c a c t c t t a c c t t c c t t c g t a c t t g c c g g t g c t c a a g

4320 EIEGEGEGRPYEGTQTAKLK FNLSFSLSPGVLAGLCGLQF g a a a t t g a g g g a g a a g g a g a g g g a c g g c c c t a c g a g g g c a c c c a g a c a g c c a a g c t g a a a c t t t a a c t c c c t c t t c c t c t c c c t g c c g g g a t g c t c c c g t g g g t c t g t c g g t t c g a c t t t

4380 VTKGGPLPFAWDILSPQFMY HCLAPRQWESPVDQAWLKHV g t g a c a a a g g g c g g g c c t c t g c c a t t c g c t t g g g a c a t c c t g a g c c c a c a g t t t a t g t a c c a c t g t t t c c c g c c c g g a g a c g g t a a g c g a a c c c t g t a g g a c t c g g g t g t c a a a t a c a t g

PsiI 4440 GSKAYVKHPADIPDYKKLSF AGLGIHFMWSVNGIILFQAE g g c t c c a a g g c c t a t g t g a a a c a t c c a g c t g a c a t t c c c g a t t a t a a g a a a c t g a g c t t c c c g a g g t t c c g g a t a c a c t t t g t a g g t c g a c t g t a a g g g c t a a t a t t c t t t g a c t c g a a g

4500 PEGFKWERVMNFEDGGLVTV GLPKLPFSHHVELVSAQHSH c c c g a g g g g t t t a a g t g g g a a a g a g t g a t g a a c t t c g a g g a c g g a g g c c t g g t g a c t g t g g g g c t c c c c a a a t t c a c c c t t t c t c a c t a c t t g a a g c t c c t g c c t c c g g a c c a c t g a c a c

KflI 4560 TQDSSLQDGTLIYKVKMRGT GLVAGQLIPGQDVLHFHSPC a c c c a g g a c a g c t c c c t g c a g g a t g g g a c c c t g a t c t a c a a g g t g a a a a t g a g a g g g a c a t g g g t c c t g t c g a g g g a c g t c c t a c c c t g g g a c t a g a t g t t c c a c t t t t a c t c t c c c t g t

4620 NFPPDGPVMQKKTMGWEAST IKGRISRHHLLFSHSPLGGG a a t t t t c c c c c t g a t g g a c c t g t g a t g c a g a a g a a a a c t a t g g g a t g g g a g g c c t c c a c c t t a a a a g g g g g a c t a c c t g g a c a c t a c g t c t t c t t t t g a t a c c c t a c c c t c c g g a g g t g g

4680 ERLYPRDGVLKGEIHQALKL FPQIWAVPHQFSFDVLSQLQ g a a a g g c t g t a t c c a c g c g a c g g g g t g c t g a a a g g a g a a a t c c a c c a g g c t c t g a a g c t g c t t t c c g a c a t a g g t g c g c t g c c c c a c g a c t t t c c t c t t t a g g t g g t c c g a g a c t t c g a c

4740 KDGGHYLVEFKTIYMAKKPV FIPSM a a a g a t g g g g g a c a t t a c c t g g t g g a g t t c a a g a c a a t c t a c a t g g c c a a g a a a c c t g t g t t t c t a c c c c c t g t a a t g g a c c a c c t c a a g t t c t g t t a g a t g t a c c g g t t c t t t g g a c a c

4800 QLPGYYYVDTKLDITSHNED c a g c t g c c a g g c t a c t a t t a c g t g g a c a c a a a a c t g g a t a t c a c t t c a c a c a a c g a g g a c g t c g a c g g t c c g a t g a t a a t g c a c c t g t g t t t t g a c c t a t a g t g a a g t g t g t t g c t c c t g

NcoI 4860 YTIVEQYERSEGRHHLFLGH t a c a c t a t t g t g g a g c a g t a t g a a c g g a g c g a g g g g a g a c a c c a t c t g t t c c t g g g c c a t a t g t g a t a a c a c c t c g t c a t a c t t g c c t c g c t c c c c t c t g t g g t a g a c a a g g a c c c g g t a

4920 GTGSTGSGSSGTASSEDNNM *ALIVIH g g g a c t g g a a g t a c c g g c t c a g g g t c t a g t g g a a c c g c c t c a a g c g a g g a t a a c a a t a t g c c c t g a c c t t c a t g g c c g a g t c c c a g a t c a c c t t g g c g g a g t t c g c t c c t a t t g t t a t a c

4980 AVIKEFMRFKVRMEGSMNGH SHDFLEHPKLHAHLAAHIPV g c t g t g a t c a a a g a g t t c a t g a g g t t t a a g g t g c g c a t g g a g g g c a g c a t g a a t g g g c a c c g a c a c t a g t t t c t c a a g t a c t c c a a a t t c c a c g c g t a c c t c c c g t c g t a c t t a c c c g t g

5040 EFEIEGEGEGRPYEGTQTAK FKLNFSLAFPPRVLACLSGL g a a t t t g a g a t t g a a g g a g a g g g c g a a g g g a g g c c t t a c g a g g g c a c a c a g a c t g c c a a g c t t a a a c t c t a a c t t c c t c t c c c g c t t c c c t c c g g a a t g c t c c c g t g t g t c t g a c g g t t c

5100 LKVTKGGPLPFAWDILSPQF QFHGLSSWQRESPIDQRRLK c t g a a a g t g a c c a a g g g a g g a c c a c t g c c t t t c g c t t g g g a t a t c c t g t c t c c t c a g t t t g a c t t t c a c t g g t t c c c t c c t g g t g a c g g a a a g c g a a c c c t a t a g g a c a g a g g a g t c a a a

5160 MYGSKAYVKHPADIPDYKKL HVSTLGIDLMGSVNRIVLFQ a t g t a c g g a a g t a a g g c c t a t g t c a a g c a t c c c g c t g a c a t t c c t g a t t a c a a g a a a c t g t a c a t g c c t t c a t t c c g g a t a c a g t t c g t a g g g c g a c t g t a a g g a c t a a t g t t c t t t g a c

BstEII 5220 SFPEGFKWERVMNFEDGGLV REWLAKLPLSHHIKFISAQH t c t t t c c c a g a g g g c t t t a a g t g g g a g a g a g t g a t g a a t t t t g a a g a t g g a g g c c t g g t g a g a a a g g g t c t c c c g a a a t t c a c c c t c t c t c a c t a c t t a a a a c t t c t a c c t c c g g a c c a c

5280 TVTQDSSLQDGTLIYKVKMR GHCLVGRQLIASQDVFDFHA a c c g t g a c a c a g g a c t c c t c t c t g c a g g a t g g c a c t c t g a t c t a c a a a g t c a a a a t g c g c t g g c a c t g t g t c c t g a g g a g a g a c g t c c t a c c g t g a g a c t a g a t g t t t c a g t t t t a c g c g

PspOMIApaI 5340 GTNFPPDGPVMQKKTMGWEA AGIKWGIPGHHLLFCHPPLG g g c a c c a a t t t t c c a c c c g a t g g g c c c g t g a t g c a g a a g a a a a c a a t g g g g t g g g a g g c c c c g t g g t t a a a a g g t g g g c t a c c c g g g c a c t a c g t c t t c t t t t g t t a c c c c a c c c t c c g g

5400 STERLYPRDGVLKGEIHQAL ASFPQIRSVSHQLAFDVLGQ a g c a c t g a a c g g c t g t a t c c t a g a g a c g g a g t g c t g a a g g g c g a a a t c c a c c a g g c c c t g t c g t g a c t t g c c g a c a t a g g a t c t c t g c c t c a c g a c t t c c c g c t t t a g g t g g t c c g g g a c

5460 KLKDGGHYLVEFKTIYMAKK LQFVAAVVQHLEFGDVHGLF a a g c t g a a a g a c g g c g g c c a c t a c c t g g t g g a g t t c a a a a c c a t c t a c a t g g c c a a g a a a t t c g a c t t t c t g c c g c c g g t g a t g g a c c a c c t c a a g t t t t g g t a g a t g t a c c g g t t c t t t

5520 PVQLPGYYYVDTKLDITSHN WHLQGAIVIHVGLQIDCGVI c c a g t g c a g c t g c c c g g c t a t t a c t a t g t g g a c a c c a a g c t g g a t a t c a c a t c c c a c a a t g g t c a c g t c g a c g g g c c g a t a a t g a t a c a c c t g t g g t t c g a c c t a t a g t g t a g g g t g t t a

5580 EDYTIVEQYERSEGRHHLFL FVVGNHFLILPRFSAVM g a a g a c t a c a c c a t t g t g g a a c a g t a t g a g a g g t c t g a a g g a c g c c a c c a t c t g t t t c t g c t t c t g a t g t g g t a a c a c c t t g t c a t a c t c t c c a g a c t t c c t g c g g t g g t a g a c a a a g a c

AscIAsiSI 5640 YGMDELYK* t a c g g c a t g g a t g a g c t g t a t a a g t a a t a a c c a a c t c c a t a a g g a t c c g c g a t c g c g g c g a t g c c g t a c c t a c t c g a c a t a t t c a t t a t t g g t t g a g g t a t t c c t a g g c g c t a g c g c c g c

FseIAcc65IKpnIMluIDraIII 5700 c g c c a c c t g g t g g c c g g c c g g t a c c a c g c g t g c g c g c t g a t c c g g c t g c t a a c a a a g c c c g c g g t g g a c c a c c g g c c g g c c a t g g t g c g c a c g c g c g a c t a g g c c g a c g a t t g t t t c g g g

BlpI 5760 T7 terminator G G C G A C T C G T T A T T G A T C G T7 Term g a a a g g a a g c t g a g t t g g c t g c t g c c a c c g c t g a g c a a t a a c t a g c a t a a c c c c t t g g g g c t t t c c t t c g a c t c a a c c g a c g a c g g t g g c g a c t c g t t a t t g a t c g t a t t g g g g a a c c c c

5820 T7 terminator c c t c t a a a c g g g t c t t g a g g g g t t t t t t g c t g a a a g g a g g a a c t a t a t c c g g a c a t c c a c g g a g a t t t g c c c a g a a c t c c c c a a a a a a c g a c t t t c c t c c t t g a t a t a g g c c t g t a g g t g

5880 a g g a c g g g t g t g g t c g c c a t g a t c g c g t a g t c g a t a g t g g c t c c a a g t a g c g a a g c g a g c t c c t g c c c a c a c c a g c g g t a c t a g c g c a t c a g c t a t c a c c g a g g t t c a t c g c t t c g c t c g

5940 a g g a c t g g g c g g c g g c c a a a g c g g t c g g a c a g t g c t c c g a g a a c g g g t g c g c a t a g a a a t t c c t g a c c c g c c g c c g g t t t c g c c a g c c t g t c a c g a g g c t c t t g c c c a c g c g t a t c t t t a

BmtINheI 6000 t g c a t c a a c g c a t a t a g c g c t a g c a g c a c g c c a t a g t g a c t g g c g a t g c t g t c g g a a t g g a c g t a g t t g c g t a t a t c g c g a t c g t c g t g c g g t a t c a c t g a c c g c t a c g a c a g c c t t a c c

6060 a c g a c a t c c c g c a a g a g g c c c g g c a g t a c c g g c a t a a c c a a g c c t a t g c c t a c a g c a t c c t g c t g t a g g g c g t t c t c c g g g c c g t c a t g g c c g t a t t g g t t c g g a t a c g g a t g t c g t a g g

6120 a g g g t g a c g g t g c c g a g g a t g a c g a t g a g c g c a t t g t t a g a t t t c a t a c a c g g t g c c t g a t c c c a c t g c c a c g g c t c c t a c t g c t a c t c g c g t a a c a a t c t a a a g t a t g t g c c a c g g a c t

BspDIClaIHindIII 6180 -10 -35 tet promoter c t g c g t t a g c a a t t t a a c t g t g a t a a a c t a c c g c a t t a a a g c t t a t c g a t g a t a a g c t g t g a c g c a a t c g t t a a a t t g a c a c t a t t t g a t g g c g t a a t t t c g a a t a g c t a c t a t t c g a c a

EcoRI 3ʹ 5ʹ 6192 -35 tet promoter c a a a c a t g a g a a g t t t g t a c t c t t

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.