Addgene pBK-VQR-BE3 Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pBK-VQR-BE3 Sequencing Result

Map View

1000 750 500 250 End (1026) SfcI (1008) AfeI (911) SmlI (874) BpuEI (859) EaeI (794) BmtI (779) NheI (775) EcoRI (595) BsaBI (568) SspI (498) EciI (493) NmeAIII (491) BpmI (480) BsmI (457) TatI (414) BglII (409) BsaI (280) PfoI * (251) MslI (242) BseRI (217) BaeGI - Bme1580I (182) BssSI - BssSαI (181) ApaLI (178) DrdI (151) BsaHI (125) EarI (38) Start (0) SV40 NLS UGI pBK-VQR-BE3 1026 bp

Sequence View

EarIStart (0) 5ʹ 3ʹ 60 * 130 VKRKKKP SGGS L SV40 NLS UGI *VKRKKKPSGGSL g g t g a t g g t g a t g a t g a c c g g t t a g a c t t t c c t c t t c t t c t t g g g a g a a c c a c c a g a g a g c c a c t a c c a c t a c t a c t g g c c a a t c t g a a a g g a g a a g a a g a a c c c t c t t g g t g g t c t c t c

120 80 75 70 65 MKIKNEGNSDQIVLAWPKYE UGI MKIKNEGNSDQIVLAWPKYE c a t c t t a a t c t t g t t c t c a c c g t t g c t a t c c t g t a t g a c c a g a g c c c a a g g c t t g t a t t c g t a g a a t t a g a a c a a g a g t g g c a a c g a t a g g a c a t a c t g g t c t c g g g t t c c g a a c a t a a g

ApaLIDrdIBsaHI 180 60 55 50 45 PADSTLLMVNEDTSEDYATH UGI PADSTLLMVNEDTSEDYATH a g g g g c g t c g c t a g t c a g a a g c a t g a c a t t c t c g t c g g t g c t c t c g t c g t a g g c g g t g t g t c c c c g c a g c g a t c a g t c t t c g t a c t g t a a g a g c a g c c a c g a g a g c a g c a t c c g c c a c a c

BseRIBssSαIBssSIBme1580IBaeGI 240 40 35 30 25 VLIDSEPKNGIVEEVEEPLM UGI VLIDSEPKNGIVEEVEEPLM c a c g a g t a t a t c g c t t t c c g g c t t g t t c c c a a t g a c t t c t t c c a c c t c c t c t g g g a g c a t g t g c t c a t a t a g c g a a a g g c c g a a c a a g g g t t a c t g a a g a a g g t g g a g g a g a c c c t c g t a

BsaIPfoI*MslI 300 20 15 10 5 LISEQIVLQKGTEKEIIDSL UGI LISEQIVLQKGTEKEIIDSL g a g g a t g g a t t c c t g g a t a a c c a g t t g c t t a c c g g t c t c c t t t t c a a t a a t a t c t g a c a g c t c c t a c c t a a g g a c c t a t t g g t c a a c g a a t g g c c a g a g g a a a a g t t a t t a t a g a c t g t c

360 NT UGI NTSGGSDGGLQSLDIRTEYL a t t a g t a g a a c c a c c a g a g t c a c c c c c a a g c t g t g a c a a a t c t a t c c g a g t t t c a t a t a a t a a t c a t c t t g g t g g t c t c a g t g g g g g t t c g a c a c t g t t t a g a t a g g c t c a a a g t a t a t t

TatIBglII 420 GTISQHILTADLVEKTSRYQ t c c c g t g a t g g a t t g g t g a a t c a g t g t c g c g t c t a g c a c c t c c t t g g t a g a t c t g t a c t g a g g g c a c t a c c t a a c c a c t t a g t c a c a g c g c a g a t c g t g g a g g a a c c a t c t a g a c a t g a c

BpmIBsmI 480 KRDITTDFYKFAAPAGLNTL t t t g c g a t c t a t c g t t g t g t c a a a a t a c t t g a a t g c g g c t g g a g c g c c g a g g t t g g t a a g a a a c g c t a g a t a g c a a c a c a g t t t t a t g a a c t t a c g c c g a c c t c g c g g c t c c a a c c a t t c

SspINmeAIIIEciI 540 TFLHIINEAQERIPKDRHKN a g t a a a c a a a t g g a t a a t a t t t t c c g c c t g c t c a c g t a t g g g t t t a t c c c t g t g c t t g t t t c a t t t g t t t a c c t a t t a t a a a a g g c g g a c g a g t g c a t a c c c a a a t a g g g a c a c g a a c a a

EcoRIBsaBI 600 YASLVKDLNADALIVRKSFE g t a t g c g c t t a a t a c t t t g t c c a g a t t g g c a t c a g c t a g g a t g a c t c t c t t a c t g a a t t c c a t a c g c g a a t t a t g a a a c a g g t c t a a c c g t a g t c g a t c c t a c t g a g a g a a t g a c t t a a g

660 SIQEIIEDLYHKHQEVFLQK c g a a a t t t g c t c t a t g a t t t c g t c g a g a t a a t g t t t g t g c t g c t c a a c a a a a a g t t g c t t g c t t t a a a c g a g a t a c t a a a g c a g c t c t a t t a c a a a c a c g a c g a g t t g t t t t t c a a c g a a

720 QENDEPSGKLKEYHSALYLF c t g t t c g t t a t c t t c a g g t g a a c c t t t c a a c t t c t c g t a a t g g g a c g c t a a a t a c a g g a a g a c a a g c a a t a g a a g t c c a c t t g g a a a g t t g a a g a g c a t t a c c c t g c g a t t t a t g t c c t t

BmtINheI 780 NVYKSPLALENGKQLEGASA a t t c a c g t a t t t a g a c g g t a g t g c g a g t t c g t t c c c c t t t t g a a g c t c t c c g g c g c t a g c t a a g t g c a t a a a t c t g c c a t c a c g c t c a a g c a a g g g g a a a a c t t c g a g a g g c c g c g a t c g

EaeI 840 LMRKRGNELEFLSYKPLKII c a a c a t c c g t t t t c g g c c a t t t t c t a a c t c a a a c a g a c t a t a c t t t g g t a g t t t a a t t a t g t t g t a g g c a a a a g c c g g t a a a a g a t t g a g t t t g t c t g a t a t g a a a c c a t c a a a t t a a t a

SmlIBpuEI 900 LDKKVEKYGKAELFDIPNKE g a g a t c c t t t t t t a c t t c c t t g t a a c c t t t c g c c t c a a g g a a g t c g a t g g g g t t c t t t t c c t c t a g g a a a a a a t g a a g g a a c a t t g g a a a g c g g a g t t c c t t c a g c t a c c c c a a g a a a a g

AfeI 960 FSSREMITIGLLEKVSKLKK a a a a g a c g a g c g c t c c a t a a t c g t t a t c c c c a a t a a t t c t t t g a c t g a c t t c a g t t t c t t t t t t c t g c t c g c g a g g t a t t a g c a a t a g g g g t t a t t a a g a a a c t g a c t g a a g t c a a a g a a

SfcI 1020 SKGKEVKAVVLVSYAVTPSV g g a t t t t c c c t t c t c a a c t t t t g c c a c t a c t a g g a c a g a a t a g g c a a c t g t a g g g c t c a c c c t a a a a g g g a a g a g t t g a a a a c g g t g a t g a t c c t g t c t t a t c c g t t g a c a t c c c g a g t g

End (1026) 3ʹ 5ʹ 1026 FG g a a g c c c t t c g g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.