Addgene pBA409 - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pBA409


Map View

M13 Reverse (9847 .. 9863) lac operator M13/pUC Reverse (9828 .. 9850) CAP binding site SfiI (9626) L4440 (9492 .. 9509) pBR322ori-F (9239 .. 9258) AhdI (8506) Amp-R (7937 .. 7956) SspI (7701) pBRforEco (7528 .. 7546) SmaI (7483) TspMI - XmaI (7481) PfoI (6786) WPRE-R (6566 .. 6586) NotI (6348) Neo-F (6204 .. 6223) RsrII (6184) NcoI (6100) SphI (6073) PflFI - Tth111I (5786) Neo-R (5594 .. 5613) KpnI (5506) Acc65I (5502) SpeI (5444) hUBCpro-F (5331 .. 5350) EBV-rev (9913 .. 9932) PmlI (290) NruI * (833) AleI (1577) EcoRI (2028) BamHI (2216) PasI (2987) EF1a-F (3365 .. 3385) GFP-R (3453 .. 3481) MluI (3744) BstZ17I (3872) SalI (4025) GFP-F (4076 .. 4096) XcmI (4277) AfeI (4338) PaqCI (5175) pBA409 10,123 bp

Sequence View

60 3' LTR t g g a a g g g c t a a t t c a c t c c c a a a g a a g a c a a g a t a t c c t t g a t c t g t g g a t c t a c c a c a a c c t t c c c g a t t a a g t g a g g g t t t c t t c t g t t c t a t a g g a a c t a g a c a c c t a g a t g g t g t

120 3' LTR c a c a a g g c t a c t t c c c t g a t t a g c a g a a c t a c a c a c c a g g g c c a g g g g t c a g a t a t c c a c g t g t t c c g a t g a a g g g a c t a a t c g t c t t g a t g t g t g g t c c c g g t c c c c a g t c t a t a g g t g

180 3' LTR t g a c c t t t g g a t g g t g c t a c a a g c t a g t a c c a g t t g a g c c a g a t a a g g t a g a a g a g g c c a a c t g g a a a c c t a c c a c g a t g t t c g a t c a t g g t c a a c t c g g t c t a t t c c a t c t t c t c c g g t

240 3' LTR a t a a a g g a g a g a a c a c c a g c t t g t t a c a c c c t g t g a g c c t g c a t g g g a t g g a t g a c c c g g t a t t t c c t c t c t t g t g g t c g a a c a a t g t g g g a c a c t c g g a c g t a c c c t a c c t a c t g g g c c

PmlI 300 3' LTR a g a g a g a a g t g t t a g a g t g g a g g t t t g a c a g c c g c c t a g c a t t t c a t c a c g t g g c c c g a g t c t c t c t t c a c a a t c t c a c c t c c a a a c t g t c g g c g g a t c g t a a a g t a g t g c a c c g g g c t c

360 3' LTR a g c t g c a t c c g g a g t a c t t c a a g a a c t g c t g a t a t c g a g c t t g c t a c a a g g g a c t t t c c g t c g a c g t a g g c c t c a t g a a g t t c t t g a c g a c t a t a g c t c g a a c g a t g t t c c c t g a a a g g c

420 3' LTR c t g g g g a c t t t c c a g g g a g g c g t g g c c t g g g c g g g a c t g g g g a g t g g c g a g c c c t c a g a t g a c c c c t g a a a g g t c c c t c c g c a c c g g a c c c g c c c t g a c c c c t c a c c g c t c g g g a g t c t a

480 3' LTR c c t g c a t a t a a g c a g c t g c t t t t t g c c t g t a c t g g g t c t c t c t g g t t a g a c c a g a t c t g a g g a c g t a t a t t c g t c g a c g a a a a a c g g a c a t g a c c c a g a g a g a c c a a t c t g g t c t a g a c t

540 3' LTR g c c t g g g a g c t c t c t g g c t a a c t a g g g a a c c c a c t g c t t a a g c c t c a a t a a a g c t t g c c t c g g a c c c t c g a g a g a c c g a t t g a t c c c t t g g g t g a c g a a t t c g g a g t t a t t t c g a a c g g a

600 3' LTR t g a g t g c t t c a a g t a g t g t g t g c c c g t c t g t t g t g t g a c t c t g g t a a c t a g a g a t c c c t c a c t c a c g a a g t t c a t c a c a c a c g g g c a g a c a a c a c a c t g a g a c c a t t g a t c t c t a g g g a g

660 3' LTR a g a c c c t t t t a g t c a g t g t g g a a a a t c t c t a g c a g t g g c g c c c g a a c a g g g a c t t g a a a g t c t g g g a a a a t c a g t c a c a c c t t t t a g a g a t c g t c a c c g c g g g c t t g t c c c t g a a c t t t c

720 HIV-1 Ψ c g a a a g g g a a a c c a g a g g a g c t c t c t c g a c g c a g g a c t c g g c t t g c t g a a g c g c g c a c g g g c t t t c c c t t t g g t c t c c t c g a g a g a g c t g c g t c c t g a g c c g a a c g a c t t c g c g c g t g c c

780 HIV-1 Ψ c a a g a g g c g a g g g g c g g c g a c t g g t g a g t a c g c c a a a a a t t t t g a c t a g c g g a g g c t a g a g t t c t c c g c t c c c c g c c g c t g a c c a c t c a t g c g g t t t t t a a a a c t g a t c g c c t c c g a t c t

NruI* 840 HIV-1 Ψ a g g a g a g a g a t g g g t g c g a g a g c g t c a g t a t t a a g c g g g g g a g a a t t a g a t c g c g a t g g g t c c t c t c t c t a c c c a c g c t c t c g c a g t c a t a a t t c g c c c c c t c t t a a t c t a g c g c t a c c c

900 a a a a a a t t c g g t t a a g g c c a g g g g g a a a g a a a a a a t a t a a a t t a a a a c a t a t a g t a t g g g t t t t t t a a g c c a a t t c c g g t c c c c c t t t c t t t t t t a t a t t t a a t t t t g t a t a t c a t a c c c

960 c a a g c a g g g a g c t a g a a c g a t t c g c a g t t a a t c c t g g c c t g t t a g a a a c a t c a g a a g g c t g t t c g t c c c t c g a t c t t g c t a a g c g t c a a t t a g g a c c g g a c a a t c t t t g t a g t c t t c c g a

1020 *I g t a g a c a a a t a c t g g g a c a g c t a c a a c c a t c c c t t c a g a c a g g a t c a g a a g a a c t t a g a t c a t c t g t t t a t g a c c c t g t c g a t g t t g g t a g g g a a g t c t g t c c t a g t c t t c t t g a a t c t a

1080 MIYYLLLGRNHADFSLSLLC c a t t a t a t a a t a c a g t a g c a a c c c t c t a t t g t g t g c a t c a a a g g a t a g a g a t a a a a g a c a g t a a t a t a t t a t g t c a t c g t t g g g a g a t a a c a c a c g t a g t t t c c t a t c t c t a t t t t c t g t

1140 WPLKLCSLPLAFCFYSWRVA c c a a g g a a g c t t t a g a c a a g a t a g a g g a a g a g c a a a a c a a a a g t a a g a c c a c c g c a c a g c g g t t c c t t c g a a a t c t g t t c t a t c t c c t t c t c g t t t t g t t t t c a t t c t g g t g g c g t g t c g

1200 MRDNWRS LPRGSIKLGPPPSILSLQLL a a g c g g c c g g c c g c t g a t c t t c a g a c c t g g a g g a g g a g a t a t g a g g g a c a a t t g g a g a a g t t c g c c g g c c g g c g a c t a g a a g t c t g g a c c t c c t c c t c t a t a c t c c c t g t t a a c c t c t t c

1260 ELYKYKVVKIEPLGVAPTKA SNYLYLTTFISGNPTAGVLA t g a a t t a t a t a a a t a t a a a g t a g t a a a a a t t g a a c c a t t a g g a g t a g c a c c c a c c a a g g c a c t t a a t a t a t t t a t a t t t c a t c a t t t t t a a c t t g g t a a t c c t c a t c g t g g g t g g t t c c g

1320 RRE KRRVVQREKRAVGIGALFLG FLLTTCLSFLATPIPAKNRP a a a g a g a a g a g t g g t g c a g a g a g a a a a a a g a g c a g t g g g a a t a g g a g c t t t g t t c c t t g g t t t c t c t t c t c a c c a c g t c t c t c t t t t t t c t c g t c a c c c t t a t c c t c g a a a c a a g g a a c c

1380 RRE FLGAAGSTMGAASMTLTVQA NKPAAPLVIPAADIVSVTCA g t t c t t g g g a g c a g c a g g a a g c a c t a t g g g c g c a g c g t c a a t g a c g c t g a c g g t a c a g g c c a a g a a c c c t c g t c g t c c t t c g t g a t a c c c g c g t c g c a g t t a c t g c g a c t g c c a t g t c c g

1440 RRE RQLLSGIVQQQNNLLRAIEA LCNNDPITCCCFLKSLAISA c a g a c a a t t a t t g t c t g g t a t a g t g c a g c a g c a g a a c a a t t t g c t g a g g g c t a t t g a g g c g t c t g t t a a t a a c a g a c c a t a t c a c g t c g t c g t c t t g t t a a a c g a c t c c c g a t a a c t c c g

1500 RRE QQHLLQLTVWGIKQLQARIL CCCRNCSVTQPM g c a a c a g c a t c t g t t g c a a c t c a c a g t c t g g g g c a t c a a g c a g c t c c a g g c a a g a a t c c t c g t t g t c g t a g a c a a c g t t g a g t g t c a g a c c c c g t a g t t c g t c g a g g t c c g t t c t t a g g a

1560 RRE AVERYLKDQQLLGIWGCSGK g g c t g t g g a a a g a t a c c t a a a g g a t c a a c a g c t c c t g g g g a t t t g g g g t t g c t c t g g a a a c c g a c a c c t t t c t a t g g a t t t c c t a g t t g t c g a g g a c c c c t a a a c c c c a a c g a g a c c t t t

AleI 1620 LICTTAVPWNASWSNKSLEQ a c t c a t t t g c a c c a c t g c t g t g c c t t g g a a t g c t a g t t g g a g t a a t a a a t c t c t g g a a c a t g a g t a a a c g t g g t g a c g a c a c g g a a c c t t a c g a t c a a c c t c a t t a t t t a g a g a c c t t g t

1680 IWNHTTWMEWDREINNYTSL g a t t t g g a a t c a c a c g a c c t g g a t g g a g t g g g a c a g a g a a a t t a a c a a t t a c a c a a g c t t c t a a a c c t t a g t g t g c t g g a c c t a c c t c a c c c t g t c t c t t t a a t t g t t a a t g t g t t c g a a

1740 1 5 KNEQELL gp41 peptide IHSLIEESQNQQEKNEQELL a a t a c a c t c c t t a a t t g a a g a a t c g c a a a a c c a g c a a g a a a a g a a t g a a c a a g a a t t a t t t t a t g t g a g g a a t t a a c t t c t t a g c g t t t t g g t c g t t c t t t t c t t a c t t g t t c t t a a t a a

1800 10 15 ELDKWASL (in frame with gp41 peptide) WNWFNITNWLWY gp41 peptide ELDKWASLWNWFNITNWLWY g g a a t t a g a t a a a t g g g c a a g t t t g t g g a a t t g g t t t a a c a t a a c a a a t t g g c t g t g g t a c c t t a a t c t a t t t a c c c g t t c a a a c a c c t t a a c c a a a t t g t a t t g t t t a a c c g a c a c c a t

1860 (in frame with gp41 peptide) IKLFIMIVGGLVGLRIVFAV IKLFIMIVGGLVGLRIVFAV t a t a a a a t t a t t c a t a a t g a t a g t a g g a g g c t t g g t a g g t t t a a g a a t a g t t t t t g c t g t a t a t t t t a a t a a g t a t t a c t a t c a t c c t c c g a a c c a t c c a a a t t c t t a t c a a a a a c g a c a

1920 (in frame with gp41 peptide) LSIVNRVRQGYSPLSFQTHL LSIVNRVRQGYSPLSFQTHL a c t t t c t a t a g t g a a t a g a g t t a g g c a g g g a t a t t c a c c a t t a t c g t t t c a g a c c c a c c t t g a a a g a t a t c a c t t a t c t c a a t c c g t c c c t a t a a g t g g t a a t a g c a a a g t c t g g g t g g a

1980 (in frame with gp41 peptide) PTPRGPDRPEGIEEEGGERD PTPRGPDRPEGIEEEGGERD c c c a a c c c c g a g g g g a c c c g a c a g g c c c g a a g g a a t a g a a g a a g a a g g t g g a g a g a g a g a g g g t t g g g g c t c c c c t g g g c t g t c c g g g c t t c c t t a t c t t c t t c t t c c a c c t c t c t c t c t

EcoRI 2040 (in frame with gp41 peptide) RDRSIRLVNGSRRYRRIHKW RDRSIRLVNGSRRYRRIHKW c a g a g a c a g a t c c a t t c g a t t a g t g a a c g g a t c t c g a c g g t a t c g c c g a a t t c a c a a a t g g t c t c t g t c t a g g t a a g c t a a t c a c t t g c c t a g a g c t g c c a t a g c g g c t t a a g t g t t t a c

2100 (in frame with gp41 peptide) QYSSTILKEKGGLGGTVQGK cPPT/CTS QYSSTILKEKGGLGGTVQGK g c a g t a t t c a t c c a c a a t t t t a a a a g a a a a g g g g g g a t t g g g g g g t a c a g t g c a g g g g a a c g t c a t a a g t a g g t g t t a a a a t t t t c t t t t c c c c c c t a a c c c c c c a t g t c a c g t c c c c t t

2160 E* cPPT/CTS E* a g a a t a g t a g a c a t a a t a g c a a c a g a c a t a c a a a c t a a a g a a t t a c a a a a a c a a a t t a c a t c t t a t c a t c t g t a t t a t c g t t g t c t g t a t g t t t g a t t t c t t a a t g t t t t t g t t t a a t g t

BamHI 2220 cPPT/CTS a a a a t t c a a a a t t t t c g g g t t t a t t a c a g g g a c a g c a g a g a t c c a g t t t g g a c t a g g a t c t t t t a a g t t t t a a a a g c c c a a a t a a t g t c c c t g t c g t c t c t a g g t c a a a c c t g a t c c t a g

2280 EF-1 α promoter c c c a g t t t g g t t a g t a c c g g g c c c g g t g c c c g t c a g t g g g c a g a g c g c a c a t c g c c c a c a g g g t c a a a c c a a t c a t g g c c c g g g c c a c g g g c a g t c a c c c g t c t c g c g t g t a g c g g g t g t

2340 EF-1 α promoter g t c c c c g a g a a g t t g g g g g g a g g g g t c g g c a a t t g a a c c g g t g c c t a g a g a a g g t g g c g c c a g g g g c t c t t c a a c c c c c c t c c c c a g c c g t t a a c t t g g c c a c g g a t c t c t t c c a c c g c g

2400 EF-1 α promoter g g g g t a a a c t g g g a a a g t g a t g t c g t g t a c t g g c t c c g c c t t t t t c c c g a g g g t g g g g g a c c c c a t t t g a c c c t t t c a c t a c a g c a c a t g a c c g a g g c g g a a a a a g g g c t c c c a c c c c c t

2460 EF-1 α promoter g a a c c g t a t a t a a g t g c a g t a g t c g c c g t g a a c g t t c t t t t t c g c a a c g g g t t t g c c g c c c t t g g c a t a t a t t c a c g t c a t c a g c g g c a c t t g c a a g a a a a a g c g t t g c c c a a a c g g c g g

2520 EF-1 α promoter EF-1 α intron A a g a a c a c a g g t a a g t g c c g t g t g t g g t t c c c g c g g g c c t g g c c t c t t t a c g g g t t a t g g c t c t t g t g t c c a t t c a c g g c a c a c a c c a a g g g c g c c c g g a c c g g a g a a a t g c c c a a t a c c g

2580 EF-1 α promoter EF-1 α intron A *KWRAATRSEQDRAE c c t t g c g t g c c t t g a a t t a c t t c c a c c t g g c t g c a g t a c g t g a t t c t t g a t c c c g a g c t t g g a a c g c a c g g a a c t t a a t g a a g g t g g a c c g a c g t c a t g c a c t a a g a a c t a g g g c t c g a a

2640 EF-1 α promoter EF-1 α intron A PQFHTPSNSAKRKLLGKAEH c g g g t t g g a a g t g g g t g g g a g a g t t c g a g g c c t t g c g c t t a a g g a g c c c c t t c g c c t c g t g c c c a a c c t t c a c c c a c c c t c t c a a g c t c c g g a a c g c g a a t t c c t c g g g g a a g c g g a g c a

2700 EF-1 α promoter EF-1 α intron A KLQPRAQASPGGRAFRTAGE g c t t g a g t t g a g g c c t g g c c t g g g c g c t g g g g c c g c c g c g t g c g a a t c t g g t g g c a c c t t c g a a c t c a a c t c c g g a c c g g a c c c g c g a c c c c g g c g g c g c a c g c t t a g a c c a c c g t g g a a

2760 EF-1 α promoter EF-1 α intron A RRDRQKRYTELWKFNKIVQQ c g c g c c t g t c t c g c t g c t t t c g a t a a g t c t c t a g c c a t t t a a a a t t t t t g a t g a c c t g c t g c g c g g a c a g a g c g a c g a a a g c t a t t c a g a g a t c g g t a a a t t t t a a a a a c t a c t g g a c g a

2820 EF-1 α promoter EF-1 α intron A SAKKRALYDQLHPGLDACQY g c g a c g c t t t t t t t c t g g c a a g a t a g t c t t g t a a a t g c g g g c c a a g a t c t g c a c a c t g g t c g c t g c g a a a a a a a g a c c g t t c t a t c a g a a c a t t t a c g c c c g g t t c t a g a c g t g t g a c c a

2880 EF-1 α promoter EF-1 α intron A KPKQPRPRRRPGHTGACMNP a t t t c g g t t t t t g g g g c c g c g g g c g g c g a c g g g g c c c g t g c g t c c c a g c g c a c a t g t t c g t a a a g c c a a a a a c c c c g g c g c c c g c c g c t g c c c c g g g c a c g c a g g g t c g c g t g t a c a a g c

2940 EF-1 α promoter EF-1 α intron A SAPGALAAVSFRVPTTELQG g c g a g g c g g g g c c t g c g a g c g c g g c c a c c g a g a a t c g g a c g g g g g t a g t c t c a a g c t g g c c g c t c c g c c c c g g a c g c t c g c g c c g g t g g c t c t t a g c c t g c c c c c a t c a g a g t t c g a c c g

PasI 3000 EF-1 α promoter EF-1 α intron A AQEPAQGRAATYRGARPPLA c g g c c t g c t c t g g t g c c t g g c c t c g c g c c g c c g t g t a t c g c c c c g c c c t g g g c g g c a a g g g c c g g a c g a g a c c a c g g a c c g g a g c g c g g c g g c a c a t a g c g g g g c g g g a c c c g c c g t t c c

3060 EF-1 α promoter EF-1 α intron A PGTPVLQTLPFIAAERGQQL c t g g c c c g g t c g g c a c c a g t t g c g t g a g c g g a a a g a t g g c c g c t t c c c g g c c c t g c t g c a g a c c g g g c c a g c c g t g g t c a a c g c a c t c g c c t t t c t a c c g g c g a a g g g c c g g g a c g a c g t

3120 EF-1 α promoter EF-1 α intron A SSLISSAASPLAPPHTVWVF g g g a g c t c a a a a t g g a g g a c g c g g c g c t c g g g a g a g c g g g c g g g t g a g t c a c c c a c a c a a c c c t c g a g t t t t a c c t c c t g c g c c g c g a g c c c t c t c g c c c g c c c a c t c a g t g g g t g t g t t

3180 EF-1 α promoter EF-1 α intron A SFPRETRLRRKM a g g a a a a g g g c c t t t c c g t c c t c a g c c g t c g c t t c a t g t g a c t c c a c g g a g t a c c g g g c g t c c t t t t c c c g g a a a g g c a g g a g t c g g c a g c g a a g t a c a c t g a g g t g c c t c a t g g c c c g c

3240 EF-1 α promoter EF-1 α intron A c c g t c c a g g c a c c t c g a t t a g t t c t c g c g c t t t t g g a g t a c g t c g t c t t t a g g t t g g g g g g g c a g g t c c g t g g a g c t a a t c a a g a g c g c g a a a a c c t c a t g c a g c a g a a a t c c a a c c c c c

3300 EF-1 α promoter EF-1 α intron A g a g g g g t t t t a t g c g a t g g a g t t t c c c c a c a c t g a g t g g g t g g a g a c t g a a g t t a g g c c a c t c c c c a a a a t a c g c t a c c t c a a a g g g g t g t g a c t c a c c c a c c t c t g a c t t c a a t c c g g t

3360 EF-1 α promoter EF-1 α intron A g c t t g g c a c t t g a t g t a a t t c t c c t t g g a a t t t g c c c t t t t t g a g t t t g g a t c t t g g t t c c g a a c c g t g a a c t a c a t t a a g a g g a a c c t t a a a c g g g a a a a a c t c a a a c c t a g a a c c a a g

3420 EF-1 α promoter EF-1 α intron A T C A A G C C T C A G A C A G T G G T T C EF1a-F a t t c t c a a g c c t c a g a c a g t g g t t c a a a g t t t t t t t c t t c c a t t t c a g g t g t c g t g a g t t t a a g a g t t c g g a g t c t g t c a c c a a g t t t c a a a a a a a g a a g g t a a a g t c c a c a g c a c t c a a

3480 1 M 5 10 15 SKGEELFTGVVPILVELD superfolder GFP 1 5 10 15 MSKGEELFTGVVPILVELD superfolder GFP C A A C A G G G T T A A G A A C A A C T T A A T C T A C GFP-R MSKGEELFTGVVPILVELD a a a t g a g c a a a g g a g a a g a a c t t t t c a c t g g a g t t g t c c c a a t t c t t g t t g a a t t a g a t g t t t a c t c g t t t c c t c t t c t t g a a a a g t g a c c t c a a c a g g g t t a a g a a c a a c t t a a t c t a c

3540 20 25 30 35 GDVNGHKFSVRGEGEGDATN superfolder GFP 20 25 30 35 GDVNGHKFSVRGEGEGDATN superfolder GFP C GFP-R GDVNGHKFSVRGEGEGDATN g t g a t g t t a a t g g g c a c a a a t t t t c t g t c c g t g g a g a g g g t g a a g g t g a t g c t a c a a a c g c a c t a c a a t t a c c c g t g t t t a a a a g a c a g g c a c c t c t c c c a c t t c c a c t a c g a t g t t t g c

3600 40 45 50 55 GKLTLKFICTTGKLPVPWPT superfolder GFP 40 45 50 55 GKLTLKFICTTGKLPVPWPT superfolder GFP GKLTLKFICTTGKLPVPWPT g a a a a c t c a c c c t t a a a t t t a t t t g c a c t a c t g g a a a a c t a c c t g t t c c g t g g c c a a c a c c t t t t g a g t g g g a a t t t a a a t a a a c g t g a t g a c c t t t t g a t g g a c a a g g c a c c g g t t g t g

3660 60 65 70 75 LVTTLTYGVQCFSRYPDHMK superfolder GFP 60 65 70 75 LVTTLTYGVQCFSRYPDHMK superfolder GFP LVTTLTYGVQCFSRYPDHMK t t g t c a c t a c t c t g a c c t a t g g t g t t c a a t g c t t t t c c c g t t a t c c g g a t c a c a t g a a a c a a c a g t g a t g a g a c t g g a t a c c a c a a g t t a c g a a a a g g g c a a t a g g c c t a g t g t a c t t t g

3720 80 85 90 95 RHDFFKSAMPEGYVQERTIS superfolder GFP 80 85 90 95 RHDFFKSAMPEGYVQERTIS superfolder GFP RHDFFKSAMPEGYVQERTIS g g c a t g a c t t t t t c a a g a g t g c c a t g c c c g a a g g t t a t g t a c a g g a a c g c a c t a t a t c t t c c g t a c t g a a a a a g t t c t c a c g g t a c g g g c t t c c a a t a c a t g t c c t t g c g t g a t a t a g a a

MluI 3780 100 105 110 115 FKDDGTYKTRAEVKFEGDTL superfolder GFP 100 105 110 115 FKDDGTYKTRAEVKFEGDTL superfolder GFP FKDDGTYKTRAEVKFEGDTL t c a a a g a t g a c g g g a c c t a c a a g a c g c g t g c t g a a g t c a a g t t t g a a g g t g a t a c c c t t g a g t t t c t a c t g c c c t g g a t g t t c t g c g c a c g a c t t c a g t t c a a a c t t c c a c t a t g g g a a c

3840 120 125 130 135 VNRIELKGIDFKEDGNILGH superfolder GFP 120 125 130 135 VNRIELKGIDFKEDGNILGH superfolder GFP VNRIELKGIDFKEDGNILGH t t a a t c g t a t c g a g t t a a a g g g t a t t g a t t t t a a a g a a g a t g g a a a c a t t c t t g g a c a c a a a t t a g c a t a g c t c a a t t t c c c a t a a c t a a a a t t t c t t c t a c c t t t g t a a g a a c c t g t g t

BstZ17I 3900 140 145 150 155 KLEYNFNSHNVYITADKQKN superfolder GFP 140 145 150 155 KLEYNFNSHNVYITADKQKN superfolder GFP KLEYNFNSHNVYITADKQKN a a c t c g a g t a c a a c t t t a a c t c a c a c a a t g t a t a c a t c a c g g c a g a c a a a c a a a a g a a t g t t g a g c t c a t g t t g a a a t t g a g t g t g t t a c a t a t g t a g t g c c g t c t g t t t g t t t t c t t a c

3960 160 165 170 175 GIKANFKIRHNVEDGSVQLA superfolder GFP 160 165 170 175 GIKANFKIRHNVEDGSVQLA superfolder GFP GIKANFKIRHNVEDGSVQLA g a a t c a a a g c t a a c t t c a a a a t t c g c c a c a a c g t t g a a g a t g g t t c c g t t c a a c t a g c a g c t t a g t t t c g a t t g a a g t t t t a a g c g g t g t t g c a a c t t c t a c c a a g g c a a g t t g a t c g t c

4020 180 185 190 195 DHYQQNTPIGDGPVLLPDNH superfolder GFP 180 185 190 195 DHYQQNTPIGDGPVLLPDNH superfolder GFP DHYQQNTPIGDGPVLLPDNH a c c a t t a t c a a c a a a a t a c t c c a a t t g g c g a t g g c c c t g t c c t t t t a c c a g a c a a c c a t t t g g t a a t a g t t g t t t t a t g a g g t t a a c c g c t a c c g g g a c a g g a a a a t g g t c t g t t g g t a a

SalI 4080 200 205 210 215 YLSTQSVLSKDPNEKRDHMV superfolder GFP 200 205 210 215 YLSTQSVLSKDPNEKRDHMV superfolder GFP G G T C C GFP-F YLSTQSVLSKDPNEKRDHMV a c c t g t c g a c a c a a t c t g t c c t t t c g a a a g a t c c c a a c g a a a a g c g t g a c c a c a t g g t c c t g g a c a g c t g t g t t a g a c a g g a a a g c t t t c t a g g g t t g c t t t t c g c a c t g g t g t a c c a g g

4140 220 225 230 235 LLEFVTAAGITHGMDELYK* superfolder GFP 220 225 230 235 LLEFVTAAGITHGMDELYK * superfolder GFP T T C T T G A G T T T G T A A C GFP-F LLEFVTAAGITHGMDELYK* t t c t t g a g t t t g t a a c t g c t g c t g g g a t t a c a c a t g g c a t g g a t g a g c t c t a c a a a t a a a a a g a a c t c a a a c a t t g a c g a c g a c c c t a a t g t g t a c c g t a c c t a c t c g a g a t g t t t a t t t

4200 1 5 YPYDVPDYA * HA a c c t c g a g t c t a g a g g g c c c g c g g t t c g a a t a c c c a t a c g a c g t c c c a g a c t a c g c t t a g t g g a g c t c a g a t c t c c c g g g c g c c a a g c t t a t g g g t a t g c t g c a g g g t c t g a t g c g a a t c

4260 t a a t g a t t a a t t a a a c t a g a a a t t c t a c c g g g t a g g g g a g g c g c t t t t c c c a a g g c a g t c a t t a c t a a t t a a t t t g a t c t t t a a g a t g g c c c a t c c c c t c c g c g a a a a g g g t t c c g t c a g

XcmI 4320 UbC promoter t g g a g t a a c c c a c c c a a g a t c t g g c c t c c g c g c c g g g t t t t g g c g c c t c c c g c g g g c g c c a c c t c a t t g g g t g g g t t c t a g a c c g g a g g c g c g g c c c a a a a c c g c g g a g g g c g c c c g c g g

AfeI 4380 UbC promoter c c c c t c c t c a c g g c g a g c g c t g c c a c g t c a g a c g a a g g g c g c a g c g a g c g t c c t g a t c c t g g g g a g g a g t g c c g c t c g c g a c g g t g c a g t c t g c t t c c c g c g t c g c t c g c a g g a c t a g g a

4440 UbC promoter t c c g c c c g g a c g c t c a g g a c a g c g g c c c g c t g c t c a t a a g a c t c g g c c t t a g a a c c c c a g a g g c g g g c c t g c g a g t c c t g t c g c c g g g c g a c g a g t a t t c t g a g c c g g a a t c t t g g g g t c

4500 UbC promoter t a t c a g c a g a a g g a c a t t t t a g g a c g g g a c t t g g g t g a c t c t a g g g c a c t g g t t t t c t t t a t a g t c g t c t t c c t g t a a a a t c c t g c c c t g a a c c c a c t g a g a t c c c g t g a c c a a a a g a a a

4560 UbC promoter c c a g a g a g c g g a a c a g g c g a g g a a a a g t a g t c c c t t c t c g g c g a t t c t g c g g a g g g a t c t g g t c t c t c g c c t t g t c c g c t c c t t t t c a t c a g g g a a g a g c c g c t a a g a c g c c t c c c t a g a

4620 UbC promoter c c g t g g g g c g g t g a a c g c c g a t g a t t a t a t a a g g a c g c g c c g g g t g t g g c a c a g c t a g t t g g c a c c c c g c c a c t t g c g g c t a c t a a t a t a t t c c t g c g c g g c c c a c a c c g t g t c g a t c a a

4680 UbC promoter c c g t c g c a g c c g g g a t t t g g g t c g c g g t t c t t g t t t g t g g a t c g c t g t g a t c g t c a c t t g g g c a g c g t c g g c c c t a a a c c c a g c g c c a a g a a c a a a c a c c t a g c g a c a c t a g c a g t g a a c

4740 UbC promoter g t g a g t a g c g g g c t g c t g g g c t g g c c g g g g c t t t c g t g g c c g c c g g g c c g c t c g g t g g g a c a c t c a t c g c c c g a c g a c c c g a c c g g c c c c g a a a g c a c c g g c g g c c c g g c g a g c c a c c c t

4800 UbC promoter c g g a a g c g t g t g g a g a g a c c g c c a a g g g c t g t a g t c t g g g t c c g c g a g c a a g g t t g c c c t g c c t t c g c a c a c c t c t c t g g c g g t t c c c g a c a t c a g a c c c a g g c g c t c g t t c c a a c g g g a

4860 UbC promoter g a a c t g g g g g t t g g g g g g a g c g c a g c a a a a t g g c g g c t g t t c c c g a g t c t t g a a t g g a a g c t t g a c c c c c a a c c c c c c t c g c g t c g t t t t a c c g c c g a c a a g g g c t c a g a a c t t a c c t t c

4920 UbC promoter a c g c t t g t g a g g c g g g c t g t g a g g t c g t t g a a a c a a g g t g g g g g g c a t g g t g g g c g g c a a t g c g a a c a c t c c g c c c g a c a c t c c a g c a a c t t t g t t c c a c c c c c c g t a c c a c c c g c c g t t

4980 UbC promoter g a a c c c a a g g t c t t g a g g c c t t c g c t a a t g c g g g a a a g c t c t t a t t c g g g t g a g a t g g g c c t t g g g t t c c a g a a c t c c g g a a g c g a t t a c g c c c t t t c g a g a a t a a g c c c a c t c t a c c c g

5040 UbC promoter t g g g g c a c c a t c t g g g g a c c c t g a c g t g a a g t t t g t c a c t g a c t g g a g a a c t c g g t t t g t a c c c c g t g g t a g a c c c c t g g g a c t g c a c t t c a a a c a g t g a c t g a c c t c t t g a g c c a a a c a

5100 UbC promoter c g t c t g t t g c g g g g g c g g c a g t t a t g g c g g t g c c g t t g g g c a g t g c a c c c g t a c c t t t g g g c a g a c a a c g c c c c c g c c g t c a a t a c c g c c a c g g c a a c c c g t c a c g t g g g c a t g g a a a c c

5160 UbC promoter g a g c g c g c g c c c t c g t c g t g t c g t g a c g t c a c c c g t t c t g t t g g c t t a t a a t g c a g g g t g c t c g c g c g c g g g a g c a g c a c a g c a c t g c a g t g g g c a a g a c a a c c g a a t a t t a c g t c c c a c

PaqCI 5220 UbC promoter g g g c c a c c t g c c g g t a g g t g t g c g g t a g g c t t t t c t c c g t c g c a g g a c g c a g g g t t c g g g c c c g g t g g a c g g c c a t c c a c a c g c c a t c c g a a a a g a g g c a g c g t c c t g c g t c c c a a g c c c

5280 UbC promoter c c t a g g g t a g g c t c t c c t g a a t c g a c a g g c g c c g g a c c t c t g g t g a g g g g a g g g a t a a g t g g a t c c c a t c c g a g a g g a c t t a g c t g t c c g c g g c c t g g a g a c c a c t c c c c t c c c t a t t c a

5340 UbC promoter T G A A G C T C C G hUBCpro-F g a g g c g t c a g t t t c t t t g g t c g g t t t t a t g t a c c t a t c t t c t t a a g t a g c t g a a g c t c c g c t c c g c a g t c a a a g a a a c c a g c c a a a a t a c a t g g a t a g a a g a a t t c a t c g a c t t c g a g g c

5400 UbC promoter G T T T T G A A C T hUBCpro-F g t t t t g a a c t a t g c g c t c g g g g t t g g c g a g t g t g t t t t g t g a a g t t t t t t a g g c a c c t t t c a a a a c t t g a t a c g c g a g c c c c a a c c g c t c a c a c a a a a c a c t t c a a a a a a t c c g t g g a a a

SpeI 5460 UbC promoter t g a a a t g t a a t c a t t t g g g t c a a t a t g t a a t t t t c a g t g t t a g a c t a g t a a a t t g t c c g c a c t t t a c a t t a g t a a a c c c a g t t a t a c a t t a a a a g t c a c a a t c t g a t c a t t t a a c a g g c g

Acc65IKpnI 5520 UbC promoter t a a a t t c t g g c c g t t t t t g g c t t t t t t g t t a g a c g a a g c t t g g t a c c g a g c t c g g a t c t c a t t t a a g a c c g g c a a a a a c c g a a a a a a c a a t c t g c t t c g a a c c a t g g c t c g a g c c t a g a g

5580 1 5 10 MIEQDGLHAGSPAA NeoR/KanR 1 5 10 MIEQDGLHAGSPAA NeoR/KanR MIEQDGLHAGSPAA c a c c c c g t a c c g g t c c a c c a t g a t t g a a c a a g a t g g a t t g c a c g c a g g t t c t c c g g c c g c g t g g g g c a t g g c c a g g t g g t a c t a a c t t g t t c t a c c t a a c g t g c g t c c a a g a g g c c g g c g

5640 15 20 25 30 WVERLFGYDWAQQTIGCSDA NeoR/KanR 15 20 25 30 WVERLFGYDWAQQTIGCSDA NeoR/KanR G A T A A G C C G A T A C T G A C C C G Neo-R WVERLFGYDWAQQTIGCSDA t t g g g t g g a g a g g c t a t t c g g c t a t g a c t g g g c a c a a c a g a c a a t c g g c t g c t c t g a t g c a a c c c a c c t c t c c g a t a a g c c g a t a c t g a c c c g t g t t g t c t g t t a g c c g a c g a g a c t a c g

5700 35 40 45 50 AVFRLSAQGRPVLFVKTDLS NeoR/KanR 35 40 45 50 AVFRLSAQGRPVLFVKTDLS NeoR/KanR AVFRLSAQGRPVLFVKTDLS c g c c g t g t t c c g g c t g t c a g c g c a g g g g c g c c c g g t t c t t t t t g t c a a g a c c g a c c t g t c g c g g c a c a a g g c c g a c a g t c g c g t c c c c g c g g g c c a a g a a a a a c a g t t c t g g c t g g a c a g

5760 55 60 65 70 GALNELQDEAARLSWLATTG NeoR/KanR 55 60 65 70 GALNELQDEAARLSWLATTG NeoR/KanR GALNELQDEAARLSWLATTG c g g t g c c c t g a a t g a a c t g c a a g a c g a g g c a g c g c g g c t a t c g t g g c t g g c c a c g a c g g g g c c a c g g g a c t t a c t t g a c g t t c t g c t c c g t c g c g c c g a t a g c a c c g a c c g g t g c t g c c c

Tth111IPflFI 5820 75 80 85 90 VPCAAVLDVVTEAGRDWLLL NeoR/KanR 75 80 85 90 VPCAAVLDVVTEAGRDWLLL NeoR/KanR VPCAAVLDVVTEAGRDWLLL c g t t c c t t g c g c a g c t g t g c t c g a c g t t g t c a c t g a a g c g g g a a g g g a c t g g c t g c t a t t g c a a g g a a c g c g t c g a c a c g a g c t g c a a c a g t g a c t t c g c c c t t c c c t g a c c g a c g a t a a

5880 95 100 105 110 GEVPGQDLLSSHLAPAEKVS NeoR/KanR 95 100 105 110 GEVPGQDLLSSHLAPAEKVS NeoR/KanR GEVPGQDLLSSHLAPAEKVS g g g c g a a g t g c c g g g g c a g g a t c t c c t g t c a t c t c a c c t t g c t c c t g c c g a g a a a g t a t c c c c g c t t c a c g g c c c c g t c c t a g a g g a c a g t a g a g t g g a a c g a g g a c g g c t c t t t c a t a g

5940 115 120 125 130 IMADAMRRLHTLDPATCPFD NeoR/KanR 115 120 125 130 IMADAMRRLHTLDPATCPFD NeoR/KanR IMADAMRRLHTLDPATCPFD c a t c a t g g c t g a t g c a a t g c g g c g g c t g c a t a c g c t t g a t c c g g c t a c c t g c c c a t t c g a g t a g t a c c g a c t a c g t t a c g c c g c c g a c g t a t g c g a a c t a g g c c g a t g g a c g g g t a a g c t

6000 135 140 145 150 HQAKHRIERARTRMEAGLVD NeoR/KanR 135 140 145 150 HQAKHRIERARTRMEAGLVD NeoR/KanR HQAKHRIERARTRMEAGLVD c c a c c a a g c g a a a c a t c g c a t c g a g c g a g c a c g t a c t c g g a t g g a a g c c g g t c t t g t c g a g g t g g t t c g c t t t g t a g c g t a g c t c g c t c g t g c a t g a g c c t a c c t t c g g c c a g a a c a g c t

6060 155 160 165 170 QDDLDEEHQGLAPAELFARL NeoR/KanR 155 160 165 170 QDDLDEEHQGLAPAELFARL NeoR/KanR QDDLDEEHQGLAPAELFARL t c a g g a t g a t c t g g a c g a a g a g c a t c a g g g g c t c g c g c c a g c c g a a c t g t t c g c c a g g c t a g t c c t a c t a g a c c t g c t t c t c g t a g t c c c c g a g c g c g g t c g g c t t g a c a a g c g g t c c g a

NcoISphI 6120 175 180 185 190 KASMPDGEDLVVTHGDACLP NeoR/KanR 175 180 185 190 KASMPDGEDLVVTHGDACLP NeoR/KanR KASMPDGEDLVVTHGDACLP c a a g g c g a g c a t g c c c g a c g g c g a g g a t c t c g t c g t g a c c c a t g g c g a t g c c t g c t t g c c g t t c c g c t c g t a c g g g c t g c c g c t c c t a g a g c a g c a c t g g g t a c c g c t a c g g a c g a a c g g

6180 195 200 205 210 NIMVENGRFSGFIDCGRLGV NeoR/KanR 195 200 205 210 NIMVENGRFSGFIDCGRLGV NeoR/KanR NIMVENGRFSGFIDCGRLGV g a a t a t c a t g g t g g a a a a t g g c c g c t t t t c t g g a t t c a t c g a c t g t g g c c g g c t g g g t g t c t t a t a g t a c c a c c t t t t a c c g g c g a a a a g a c c t a a g t a g c t g a c a c c g g c c g a c c c a c a

RsrII 6240 215 220 225 230 ADRYQDIALATRDIAEELGG NeoR/KanR 215 220 225 230 ADRYQDIALATRDIAEELGG NeoR/KanR C G T T G G C T A C C C G T G A T A T T Neo-F ADRYQDIALATRDIAEELGG g g c g g a c c g c t a t c a g g a c a t a g c g t t g g c t a c c c g t g a t a t t g c t g a a g a g c t t g g c g g c c g c c t g g c g a t a g t c c t g t a t c g c a a c c g a t g g g c a c t a t a a c g a c t t c t c g a a c c g c c

6300 235 240 245 250 EWADRFLVLYGIAAPDSQRI NeoR/KanR 235 240 245 250 EWADRFLVLYGIAAPDSQRI NeoR/KanR EWADRFLVLYGIAAPDSQRI c g a a t g g g c t g a c c g c t t c c t c g t g c t t t a c g g t a t c g c c g c t c c c g a t t c g c a g c g c a t g c t t a c c c g a c t g g c g a a g g a g c a c g a a a t g c c a t a g c g g c g a g g g c t a a g c g t c g c g t a

NotI 6360 255 260 265 AFYRLLDEFF* NeoR/KanR 255 260 265 AFYRLLDEFF* NeoR/KanR AFYRLLDEFF* c g c c t t c t a t c g c c t t c t t g a c g a g t t c t t c t g a t g t a c a a g t a a a g c g g c c g c g a c t c t g c g g a a g a t a g c g g a a g a a c t g c t c a a g a a g a c t a c a t g t t c a t t t c g c c g g c g c t g a g a

6420 a g a t c a t a a t c a g c c a t a c c a c a t t t g t a g a g g t t t t a c t t g c t t t a a a a a a c c t c c c a c t c t a g t a t t a g t c g g t a t g g t g t a a a c a t c t c c a a a a t g a a c g a a a t t t t t t g g a g g g t g

6480 a c c t c c c c c t g a a c c t g a a a c a t a a a a t g a a t g c a a t t g t t g t t g t t t a g t c c c t c c c a a t g g a g g g g g a c t t g g a c t t t g t a t t t t a c t t a c g t t a a c a a c a a c a a a t c a g g g a g g g t t

6540 WPRE t t c g a t a t c a a g c t t a t c g a t c g a t a g a t c c t a a t c a a c c t c t g g a t t a c a a a a t t t g t g a a g c t a t a g t t c g a a t a g c t a g c t a t c t a g g a t t a g t t g g a g a c c t a a t g t t t t a a a c a c

6600 WPRE A C A A C G A G G A A A A T G C G A T A C WPRE-R a a a g a t t g a c t g g t a t t c t t a a c t a t g t t g c t c c t t t t a c g c t a t g t g g a t a c g c t g c t t t t t c t a a c t g a c c a t a a g a a t t g a t a c a a c g a g g a a a a t g c g a t a c a c c t a t g c g a c g a a

6660 WPRE MLLLPVWLSFSPPCI MPLYHAIASRMAFIFSSLY t a a t g c c t t t g t a t c a t g c t a t t g c t t c c c g t a t g g c t t t c a t t t t c t c c t c c t t g t a t a a t t a c g g a a a c a t a g t a c g a t a a c g a a g g g c a t a c c g a a a g t a a a a g a g g a g g a a c a t a t

6720 WPRE NPGCCLFMRSCGPLSGNVAW KSWLLSLYEELWPVVRQRGV a a t c c t g g t t g c t g t c t c t t t a t g a g g a g t t g t g g c c c g t t g t c a g g c a a c g t g g c g t g g t t a g g a c c a a c g a c a g a g a a a t a c t c c t c a a c a c c g g g c a a c a g t c c g t t g c a c c g c a c c

6780 WPRE CALCLLTQPPLVGALPPPVS VCTVFADATPTGWGIATTCQ t g t g c a c t g t g t t t g c t g a c g c a a c c c c c a c t g g t t g g g g c a t t g c c a c c a c c t g t c a g c a c a c g t g a c a c a a a c g a c t g c g t t g g g g g t g a c c a a c c c c g t a a c g g t g g t g g a c a g t c g

PfoI 6840 WPRE SFPGLSLSPSLLPRRNSSPP LLSGTFAFPLPIATAELIAA t c c t t t c c g g g a c t t t c g c t t t c c c c c t c c c t a t t g c c a c g g c g g a a c t c a t c g c c g c c t a g g a a a g g c c c t g a a a g c g a a a g g g g g a g g g a t a a c g g t g c c g c c t t g a g t a g c g g c g g a

6900 WPRE ALPAAGQGLGCWALTIPWCC CLARCWTGARLLGTDNSVVL g c c t t g c c c g c t g c t g g a c a g g g g c t c g g c t g t t g g g c a c t g a c a a t t c c g t g g t g t t g t c g g a a c g g g c g a c g a c c t g t c c c c g a g c c g a c a a c c c g t g a c t g t t a a g g c a c c a c a a c a

6960 WPRE (in frame with Factor Xa site) *RGKRPQEGTNGGPNQA RGNHRPFLGCSPVLPPGFCA SGKSSSFPWLLACVATWILR c g g g g a a a t c a t c g t c c t t t c c t t g g c t g c t c g c c t g t g t t g c c a c c t g g a t t c t g c g c g g c c c c t t t a g t a g c a g g a a a g g a a c c g a c g a g c g g a c a c a a c g g t g g a c c t a a g a c g c g c

7020 WPRE (in frame with Factor Xa site) PRGEAVDR 1 RGEI Factor Xa site GRPSATSLRPSIQRTFLPAA GTSFCYVPSALNPADLPSRG g g a c g t c c t t c t g c t a c g t c c c t t c g g c c c t c a a t c c a g c g g a c c t t c c t t c c c g c g g c c c c t g c a g g a a g a c g a t g c a g g g a a g c c g g g a g t t a g g t c g c c t g g a a g g a a g g g c g c c g g

7080 WPRE CCRLCGLFRVFAFALRRVGS LLPALRPLPRLRLRPQTSRI t g c t g c c g g c t c t g c g g c c t c t t c c g c g t c t t c g c c t t c g c c c t c a g a c g a g t c g g a t c t a c g a c g g c c g a g a c g c c g g a g a a g g c g c a g a a g c g g a a g c g g g a g t c t g c t c a g c c t a g a

7140 WPRE PFGPPPRLRSFKTNDLQGSC SLWAASPPEIL* c c c t t t g g g c c g c c t c c c c g c c t g a g a t c c t t t a a g a c c a a t g a c t t a c a a g g c a g c t g t g g g a a a c c c g g c g g a g g g g c g g a c t c t a g g a a a t t c t g g t t a c t g a a t g t t c c g t c g a c a

7200 3' LTR ( Δ U3) RS* a g a t c t t a g c c a c t t t t t a a a a g a a a a g g g g g g a c t g g a a g g g c t a a t t c a c t c c c a a c g t c t a g a a t c g g t g a a a a a t t t t c t t t t c c c c c c t g a c c t t c c c g a t t a a g t g a g g g t t g c

7260 3' LTR ( Δ U3) a a g a c a a g a t c t g c t t t t t g c t t g t a c t g g g t c t c t c t g g t t a g a c c a g a t c t g a g c c t g t t c t g t t c t a g a c g a a a a a c g a a c a t g a c c c a g a g a g a c c a a t c t g g t c t a g a c t c g g a c

7320 3' LTR ( Δ U3) g g a g c t c t c t g g c t a a c t a g g g a a c c c a c t g c t t a a g c c t c a a t a a a g c t t g c c t t g a g t c c t c g a g a g a c c g a t t g a t c c c t t g g g t g a c g a a t t c g g a g t t a t t t c g a a c g g a a c t c a

7380 3' LTR ( Δ U3) g c t t c a a g t a g t g t g t g c c c g t c t g t t g t g t g a c t c t g g t a a c t a g a g a t c c c t c a g a c c c g a a g t t c a t c a c a c a c g g g c a g a c a a c a c a c t g a g a c c a t t g a t c t c t a g g g a g t c t g g

7440 3' LTR ( Δ U3) c t t t t a g t c a g t g t g g a a a a t c t c t a g c a g t a g t a g t t c a t g t c a t c t t a t t a t t c a g t a g a a a a t c a g t c a c a c c t t t t a g a g a t c g t c a t c a t c a a g t a c a g t a g a a t a a t a a g t c a t

TspMIXmaISmaI 7500 t t t a t a a c t t g c a a a g a a a t g a a t a t c a g a g a g t g a g a g g c c c g g g t t a a t t a a g g a a a g a a a t a t t g a a c g t t t c t t t a c t t a t a g t c t c t c a c t c t c c g g g c c c a a t t a a t t c c t t t c

7560 C G G A G C A C T A T G C G G A T A A pBRforEco g g c t a g a t c a t t c t t g a a g a c g a a a g g g c c t c g t g a t a c g c c t a t t t t t a t a g g t t a a t g c c g a t c t a g t a a g a a c t t c t g c t t t c c c g g a g c a c t a t g c g g a t a a a a a t a t c c a a t t a c

7620 AmpR promoter t c a t g a t a a t a a t g g t t t c t t a g a c g t c a g g t g g c a c t t t t c g g g g a a a t g t g c g c g g a a a g t a c t a t t a t t a c c a a a g a a t c t g c a g t c c a c c g t g a a a a g c c c c t t t a c a c g c g c c t t

7680 AmpR promoter c c c c t a t t t g t t t a t t t t t c t a a a t a c a t t c a a a t a t g t a t c c g c t c a t g a g a c a a t a a c g g g g a t a a a c a a a t a a a a a g a t t t a t g t a a g t t t a t a c a t a g g c g a g t a c t c t g t t a t t g

SspI 7740 1 5 signal sequence MSIQHFR AmpR promoter AmpR MSIQHFR c c t g a t a a a t g c t t c a a t a a t a t t g a a a a a g g a a g a g t a t g a g t a t t c a a c a t t t c c g t g g g a c t a t t t a c g a a g t t a t t a t a a c t t t t t c c t t c t c a t a c t c a t a a g t t g t a a a g g c a c

7800 10 15 20 signal sequence VALIPFFAAFCLPVFA 25 HPET AmpR VALIPFFAAFCLPVFAHPET t c g c c c t t a t t c c c t t t t t t g c g g c a t t t t g c c t t c c t g t t t t t g c t c a c c c a g a a a c g c a g c g g g a a t a a g g g a a a a a a c g c c g t a a a a c g g a a g g a c a a a a a c g a g t g g g t c t t t g c g

7860 30 35 40 45 LVKVKDAEDQLGARVGYIEL AmpR LVKVKDAEDQLGARVGYIEL t g g t g a a a g t a a a a g a t g c t g a a g a t c a g t t g g g t g c a c g a g t g g g t t a c a t c g a a c t g g a c c a c t t t c a t t t t c t a c g a c t t c t a g t c a a c c c a c g t g c t c a c c c a a t g t a g c t t g a c c

7920 50 55 60 65 DLNSGKILESFRPEERFPMM AmpR DLNSGKILESFRPEERFPMM a t c t c a a c a g c g g t a a g a t c c t t g a g a g t t t t c g c c c c g a a g a a c g t t t t c c a a t g a t g a t a g a g t t g t c g c c a t t c t a g g a a c t c t c a a a a g c g g g g c t t c t t g c a a a a g g t t a c t a c t

7980 70 75 80 85 STFKVLLCGAVLSRVDAGQE AmpR C G A T A C A C C G C G C C A T A A T A Amp-R STFKVLLCGAVLSRVDAGQE g c a c t t t t a a a g t t c t g c t a t g t g g c g c g g t a t t a t c c c g t g t t g a c g c c g g g c a a g a g c c g t g a a a a t t t c a a g a c g a t a c a c c g c g c c a t a a t a g g g c a c a a c t g c g g c c c g t t c t c g

8040 90 95 100 105 QLGRRIHYSQNDLVEYSPVT AmpR QLGRRIHYSQNDLVEYSPVT a a c t c g g t c g c c g c a t a c a c t a t t c t c a g a a t g a c t t g g t t g a g t a c t c a c c a g t c a c a g t t g a g c c a g c g g c g t a t g t g a t a a g a g t c t t a c t g a a c c a a c t c a t g a g t g g t c a g t g t c

8100 110 115 120 125 EKHLTDGMTVRELCSAAITM AmpR EKHLTDGMTVRELCSAAITM a a a a g c a t c t t a c g g a t g g c a t g a c a g t a a g a g a a t t a t g c a g t g c t g c c a t a a c c a t g a t t t t c g t a g a a t g c c t a c c g t a c t g t c a t t c t c t t a a t a c g t c a c g a c g g t a t t g g t a c t

8160 130 135 140 145 SDNTAANLLLTTIGGPKELT AmpR SDNTAANLLLTTIGGPKELT g t g a t a a c a c t g c g g c c a a c t t a c t t c t g a c a a c g a t c g g a g g a c c g a a g g a g c t a a c c g c a c t a t t g t g a c g c c g g t t g a a t g a a g a c t g t t g c t a g c c t c c t g g c t t c c t c g a t t g g c

8220 150 155 160 165 AFLHNMGDHVTRLDRWEPEL AmpR AFLHNMGDHVTRLDRWEPEL c t t t t t t g c a c a a c a t g g g g g a t c a t g t a a c t c g c c t t g a t c g t t g g g a a c c g g a g c t g a g a a a a a a c g t g t t g t a c c c c c t a g t a c a t t g a g c g g a a c t a g c a a c c c t t g g c c t c g a c t

8280 170 175 180 185 NEAIPNDERDTTMPVAMATT AmpR NEAIPNDERDTTMPVAMATT a t g a a g c c a t a c c a a a c g a c g a g c g t g a c a c c a c g a t g c c t g t a g c a a t g g c a a c a a c g t t a c t t c g g t a t g g t t t g c t g c t c g c a c t g t g g t g c t a c g g a c a t c g t t a c c g t t g t t g c a

8340 190 195 200 205 LRKLLTGELLTLASRQQLID AmpR LRKLLTGELLTLASRQQLID t g c g c a a a c t a t t a a c t g g c g a a c t a c t t a c t c t a g c t t c c c g g c a a c a a t t a a t a g a c t a c g c g t t t g a t a a t t g a c c g c t t g a t g a a t g a g a t c g a a g g g c c g t t g t t a a t t a t c t g a

8400 210 215 220 225 WMEADKVAGPLLRSALPAGW AmpR WMEADKVAGPLLRSALPAGW g g a t g g a g g c g g a t a a a g t t g c a g g a c c a c t t c t g c g c t c g g c c c t t c c g g c t g g c t g g t c c t a c c t c c g c c t a t t t c a a c g t c c t g g t g a a g a c g c g a g c c g g g a a g g c c g a c c g a c c a

8460 230 235 240 245 FIADKSGAGERGSRGIIAAL AmpR FIADKSGAGERGSRGIIAAL t t a t t g c t g a t a a a t c t g g a g c c g g t g a g c g t g g g t c t c g c g g t a t c a t t g c a g c a c t g g a a t a a c g a c t a t t t a g a c c t c g g c c a c t c g c a c c c a g a g c g c c a t a g t a a c g t c g t g a c c

AhdI 8520 250 255 260 265 GPDGKPSRIVVIYTTGSQAT AmpR GPDGKPSRIVVIYTTGSQAT g g c c a g a t g g t a a g c c c t c c c g t a t c g t a g t t a t c t a c a c g a c g g g g a g t c a g g c a a c t a c c g g t c t a c c a t t c g g g a g g g c a t a g c a t c a a t a g a t g t g c t g c c c c t c a g t c c g t t g a t

8580 270 275 280 285 MDERNRQIAEIGASLIKHW* AmpR MDERNRQIAEIGASLIKHW* t g g a t g a a c g a a a t a g a c a g a t c g c t g a g a t a g g t g c c t c a c t g a t t a a g c a t t g g t a a c a c c t a c t t g c t t t a t c t g t c t a g c g a c t c t a t c c a c g g a g t g a c t a a t t c g t a a c c a t t g

8640 t g t c a g a c c a a g t t t a c t c a t a t a t a c t t t a g a t t g a t t t a a a a c t t c a t t t t t a a t t t a a c a g t c t g g t t c a a a t g a g t a t a t a t g a a a t c t a a c t a a a t t t t g a a g t a a a a a t t a a a t

8700 a a a g g a t c t a g g t g a a g a t c c t t t t t g a t a a t c t c a t g a c c a a a a t c c c t t a a c g t g a g t t t t c c t a g a t c c a c t t c t a g g a a a a a c t a t t a g a g t a c t g g t t t t a g g g a a t t g c a c t c a

8760 ori t t t c g t t c c a c t g a g c g t c a g a c c c c g t a g a a a a g a t c a a a g g a t c t t c t t g a g a t c c t t a a a g c a a g g t g a c t c g c a g t c t g g g g c a t c t t t t c t a g t t t c c t a g a a g a a c t c t a g g a a

8820 ori t t t t t c t g c g c g t a a t c t g c t g c t t g c a a a c a a a a a a a c c a c c g c t a c c a g c g g t g g t t t a a a a a g a c g c g c a t t a g a c g a c g a a c g t t t g t t t t t t t g g t g g c g a t g g t c g c c a c c a a a

8880 ori g t t t g c c g g a t c a a g a g c t a c c a a c t c t t t t t c c g a a g g t a a c t g g c t t c a g c a g a g c g c c a a a c g g c c t a g t t c t c g a t g g t t g a g a a a a a g g c t t c c a t t g a c c g a a g t c g t c t c g c g

8940 ori a g a t a c c a a a t a c t g t t c t t c t a g t g t a g c c g t a g t t a g g c c a c c a c t t c a a g a a c t c t g t c t a t g g t t t a t g a c a a g a a g a t c a c a t c g g c a t c a a t c c g g t g g t g a a g t t c t t g a g a c

9000 ori t a g c a c c g c c t a c a t a c c t c g c t c t g c t a a t c c t g t t a c c a g t g g c t g c t g c c a g t g g c g a t c g t g g c g g a t g t a t g g a g c g a g a c g a t t a g g a c a a t g g t c a c c g a c g a c g g t c a c c g c

9060 ori a t a a g t c g t g t c t t a c c g g g t t g g a c t c a a g a c g a t a g t t a c c g g a t a a g g c g c a g c g g t t a t t c a g c a c a g a a t g g c c c a a c c t g a g t t c t g c t a t c a a t g g c c t a t t c c g c g t c g c c a

9120 ori c g g g c t g a a c g g g g g g t t c g t g c a c a c a g c c c a g c t t g g a g c g a a c g a c c t a c a c c g a a c g c c c g a c t t g c c c c c c a a g c a c g t g t g t c g g g t c g a a c c t c g c t t g c t g g a t g t g g c t t g

9180 ori t g a g a t a c c t a c a g c g t g a g c t a t g a g a a a g c g c c a c g c t t c c c g a a g g g a g a a a g g c g g a c t c t a t g g a t g t c g c a c t c g a t a c t c t t t c g c g g t g c g a a g g g c t t c c c t c t t t c c g c c

9240 ori G G pBR322ori-F a c a g g t a t c c g g t a a g c g g c a g g g t c g g a a c a g g a g a g c g c a c g a g g g a g c t t c c a g g g g t g t c c a t a g g c c a t t c g c c g t c c c a g c c t t g t c c t c t c g c g t g c t c c c t c g a a g g t c c c c

9300 ori G A A A C G C C T G G T A T C T T T pBR322ori-F g a a a c g c c t g g t a t c t t t a t a g t c c t g t c g g g t t t c g c c a c c t c t g a c t t g a g c g t c g a t c t t t g c g g a c c a t a g a a a t a t c a g g a c a g c c c a a a g c g g t g g a g a c t g a a c t c g c a g c t a

9360 ori t t t t g t g a t g c t c g t c a g g g g g g c g g a g c c t a t g g a a a a a c g c c a g c a a c g c g g c c t t t t a a a a c a c t a c g a g c a g t c c c c c c g c c t c g g a t a c c t t t t t g c g g t c g t t g c g c c g g a a a a

9420 t a c g g t t c c t g g c c t t t t g c t g g c c t t t t g c t c a c a t g t t c t t t c c t g c g t t a t c c c c t g a t g c c a a g g a c c g g a a a a c g a c c g g a a a a c g a g t g t a c a a g a a a g g a c g c a a t a g g g g a c

9480 a t t c t g t g g a t a a c c g t a t t a c c g c c t t t g a g t g a g c t g a t a c c g c t c g c c g c a g c c g a a t a a g a c a c c t a t t g g c a t a a t g g c g g a a a c t c a c t c g a c t a t g g c g a g c g g c g t c g g c t t

9540 A G C G A G T C A G T G A G C G A G L4440 c g a c c g a g c g c a g c g a g t c a g t g a g c g a g g a a g c g g a a g a g c g c c c a a t a c g c a a a c c g c g c t g g c t c g c g t c g c t c a g t c a c t c g c t c c t t c g c c t t c t c g c g g g t t a t g c g t t t g g c g

9600 c t c t c c c c g c g c g t t g g c c g a t t c a t t a a t g c a g c a a g c t c a t g g c t g a c t a a t t t t t t t g a g a g g g g c g c g c a a c c g g c t a a g t a a t t a c g t c g t t c g a g t a c c g a c t g a t t a a a a a a a

SfiI 9660 t a t t t a t g c a g a g g c c g a g g c c g c c t c g g c c t c t g a g c t a t t c c a g a a g t a g t g a g g a g g a t a a a t a c g t c t c c g g c t c c g g c g g a g c c g g a g a c t c g a t a a g g t c t t c a t c a c t c c t c c

9720 c t t t t t t g g a g g c c t a g g c t t t t g c a a a a a g c t c c c c g t g g c a c g a c a g g t t t c c c g a c t g a a a a a a c c t c c g g a t c c g a a a a c g t t t t t c g a g g g g c a c c g t g c t g t c c a a a g g g c t g a

9780 CAP binding site g g a a a g c g g g c a g t g a g c g c a a c g c a a t t a a t g t g a g t t a g c t c a c t c a t t a g g c a c c c c c c t t t c g c c c g t c a c t c g c g t t g c g t t a a t t a c a c t c a a t c g a g t g a g t a a t c c g t g g g g

9840 -35 -10 lac promoter lac operator A G C G G A T A A C A A T M13/pUC Reverse a g g c t t t a c a c t t t a t g c t t c c g g c t c g t a t g t t g t g t g g a a t t g t g a g c g g a t a a c a a t t c c g a a a t g t g a a a t a c g a a g g c c g a g c a t a c a a c a c a c c t t a a c a c t c g c c t a t t g t t a

9900 M13 rev T T C A C A C A G G M13/pUC Reverse C A G G A A A C A G C T A T G A C M13 Reverse t t c a c a c a g g a a a c a g c t a t g a c a t g a t t a c g a a t t t c a c a a a t a a a g c a t t t t t t t c a c a a g t g t g t c c t t t g t c g a t a c t g t a c t a a t g c t t a a a g t g t t t a t t t c g t a a a a a a a g t g

9960 G T G G T T T G T C C A A A C T C A T C EBV-rev t g c a t t c t a g t t g t g g t t t g t c c a a a c t c a t c a a t g t a t c t t a t c a t g t c t g g a t c a a c t a c g t a a g a t c a a c a c c a a a c a g g t t t g a g t a g t t a c a t a g a a t a g t a c a g a c c t a g t t g a

10,020 g g a t a a c t c a a g c t a a c c a a a a t c a t c c c a a a c t t c c c a c c c c a t a c c c t a t t a c c a c t g c c t a t t g a g t t c g a t t g g t t t t a g t a g g g t t t g a a g g g t g g g g t a t g g g a t a a t g g t g a c

10,080 c c a a t t a c c t g t g g t t t c a t t t a c t c t a a a c c t g t g a t t c c t c t g a a t t a t t t t c a t t t t g g t t a a t g g a c a c c a a a g t a a a t g a g a t t t g g a c a c t a a g g a g a c t t a a t a a a a g t a a a a

10,123 a a a g a a a t t g t a t t t g t t a a a t a t g t a c t a c a a a c t t a g t a g t t t t c t t t a a c a t a a a c a a t t t a t a c a t g a t g t t t g a a t c a t c a

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.