Addgene p1G108CFa Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: p1G108CFa Sequencing Result


Map View

1000 750 500 250 End (1018) BciVI (1005) XmnI (1002) BstAPI (946) BsrDI (944) AclI (855) BssSI BssSαI (737) BsrBI (681) NmeAIII (617) MmeI (565) RsrII (538) BsaHI (526) BssHII (495) BsgI (486) BspHI (424) BbsI (413) NspI - SphI (348) BclI * (271) Esp3I - BsmBI (248) BanI (226) BfuAI - PaqCI - BspMI (214) PstI (151) SfcI (147) EcoP15I (116) BmgBI (113) AflIII (110) PspFI (106) BseYI (102) BamHI (93) EcoRI (87) SacI - BanII (85) Eco53kI (83) HincII (76) AccI (75) SalI (74) HindIII (68) BsiEI (64) NotI - EaeI - EagI (61) SmlI - AvaI - XhoI - PspXI - BsoBI - PaeR7I (53) Start (0) 6xHis p1G108CFa 1018 bp

Sequence View

PaeR7IBsoBIPspXIXhoISmlIAvaIStart (0) 5ʹ 3ʹ 60 * 5 1 HHHHHH 6xHis c t t c c t t t c g g g c t t t g t t a g c a g c c g g a t c t c a g t g g t g g t g g t g g t g g t g c t c g a g t g g a a g g a a a g c c c g a a a c a a t c g t c g g c c t a g a g t c a c c a c c a c c a c c a c c a c g a g c t c a c

BseYIPspFIAflIIIBmgBIEagIEaeINotIBsiEIHindIIISalIAccIHincIIEco53kIBanIISacIEcoRIBamHI 120 *GAWVDK c g g c c g c a a g c t t g t c g a c g g a g c t c g a a t t c g g a t c c t t a c c c a g c c c a c a c g t c t t t t g c c g g c g t t c g a a c a g c t g c c t c g a g c t t a a g c c t a g g a a t g g g t c g g g t g t g c a g a a a a

PstISfcI 180 AYRGKEELQQLWLRADAESV g c g t a t c g g c c t t t t t c t t c t a g c t g c t g c a g c c a t a a g c g a g c g t c t g c t t c a c t c a c t c g c a t a g c c g g a a a a a g a a g a t c g a c g a c g t c g g t a t t c g c t c g c a g a c g a a g t g a g t g a

BanIBspMIPaqCIBfuAI 240 QHVDAYSKMLTAEVAPAMQS t g g t g a a c g t c a g c a t a g c t t t t c a t a a g c g t t g c t t c a a c g g c a g g t g c c a t t t g g c t t a c c a c t t g c a g t c g t a t c g a a a a g t a t t c g c a a c g a a g t t g c c g t c c a c g g t a a a c c g a a

BclI*BsmBIEsp3I 300 GDGCIYFHAGQDLLEILKKG c c g t c t c c g c a a a t a t a g a a g t g c g c t c c t t g a t c a a g a a g t t c a a t c a a t t t c t t g c c g g g c a g a g g c g t t t a t a t c t t c a c g c g a g g a a c t a g t t c t t c a a g t t a g t t a a a g a a c g g c

SphINspI 360 DQEMVHQVYTKPQNPMRSFA t c t t g t t c c a t t a c g t g c t g a a c g t a t g t t t t c g g c t g a t t t g g c a t g c g a g a a a a a g c g a g a a c a a g g t a a t g c a c g a c t t g c a t a c a a a a g c c g a c t a a a c c g t a c g c t c t t t t t c g c

BbsI 420 THLTIIGESQANELEEQYLY g t a t g a a g c g t a a t g a t g c c t t c g c t t t g g g c g t t t t c a a g c t c t t c t t g a t a c a g a t a g c a t a c t t c g c a t t a c t a c g g a a g c g a a a c c c g c a a a a g t t c g a g a a g a a c t a t g t c t a t c

BspHI 480 DEHPSRCGFYLHAEGLSQGQ t c t t c a t g a g g t g a a c g g c a g c c g a a g t a t a a a t g t g c t t c t c c a a g t g a c t g t c c t t g t a g a a g t a c t c c a c t t g c c g t c g g c t t c a t a t t t a c a c g a a g a g g t t c a c t g a c a g g a a c a

RsrIIBsaHIBssHIIBsgI 540 EKLQKRAQVFGRFPAVGTGP t c t t t t a g c t g t t t g c g c g c c t g c a c a a a g c c t c t a a a c g g c g c g a c g c c t g t t c c c g g t a g a a a a t c g a c a a a c g c g c g g a c g t g t t t c g g a g a t t t g c c g c g c t g c g g a c a a g g g c c a

MmeI 600 GVMILPTEPDKPLTFESQPT c c g a c c a t g a t a a g c g g c g t t t c a g g g t c t t t t g g c a g c g t a a a t t c t g a c t g c g g t g t g g g c t g g t a c t a t t c g c c g c a a a g t c c c a g a a a a c c g t c g c a t t t a a g a c t g a c g c c a c a c

NmeAIII 660 SIFCTITDGEQLEALYNSAI g a a a t a a a g c a c g t a a t c g t a t c t c c t t c t t g c a g c t c g g c a a g a t a g t t c g a c g c a a t t c t t t a t t t c g t g c a t t a g c a t a g a g g a a g a a c g t c g a g c c g t t c t a t c a a g c t g c g t t a a

BsrBI 720 GKYEGYGSWAEGSVVSVTIS c c t t t a t a t t c t c c a t a t c c g c t c c a c g c t t c t c c t g a g a c a a c g c t g a c c g t g a t g c t t g g a a a t a t a a g a g g t a t a g g c g a g g t g c g a a g a g g a c t c t g t t g c g a c t g g c a c t a c g a a

BssSαIBssSI 780 AQKEDVRPSSSISYYRPRIS g c t t g t t t t t c a t c g a c a c g a g g t g a t g a a g a a a t c g a g t a a t a g c g c g g g c g t a t g c t t c g a a c a a a a a g t a g c t g t g c t c c a c t a c t t c t t t a g c t c a t t a t c g c g c c c g c a t a c g a a

840 PLLAIFESFKMESAPYKELL g g c a g a a g g g c g a t a a a t t c g c t g a a t t t c a t t t c g c t c g c c g g g t a t t t t t c a a g c a g t c c g t c t t c c c g c t a t t t a a g c g a c t t a a a g t a a a g c g a g c g g c c c a t a a a a a g t t c g t c a

AclI 900 ELMTLRKALVQEKYAQKELL t c a a g c a t t g t t a a a c g t t t t g c c a g c a c t t g t t c t t t g t a g g c t t g c t t t t c a a g c a a g a g t t c g t a a c a a t t t g c a a a a c g g t c g t g a a c a a g a a a c a t c c g a a c g a a a a g t t c g t t c

BsrDIBstAPI 960 AELEVKHPPSVTKAAMARLQ g c t t c a a g c t c t a c t t t a t g c g g c g g g c t g a c c g t t t t a g c a g c c a t t g c g c g a a g c t g c c g a a g t t c g a g a t g a a a t a c g c c g c c c g a c t g g c a a a a t c g t c g g t a a c g c g c t t c g a c g

End (1018) XmnIBciVI 3ʹ 5ʹ 1018 TRTVPDQLEVYQLLEEVSV g t g c g c g t a a c a g g a t c t t g a a g c t c c a c g t a t t g c a g a a g c t c t t c t a c g g a t a c t g c a c g c g c a t t g t c c t a g a a c t t c g a g g t g c a t a a c g t c t t c g a g a a g a t g c c t a t g a c

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.