Addgene pLenti-L3US2-RFP-HDAC3 Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pLenti-L3US2-RFP-HDAC3 Sequencing Result


Map View

800 600 400 200 End (931) Puro-R (911 .. 930) TaqII (929) BseRI (908) BmgBI (889) NaeI (883) BsrFI - NgoMIV (881) PluTI - HaeII (855) SfoI (853) NarI (852) KasI (851) BmtI (843) NheI (839) BmrI (809) AlwNI (780) MscI (773) EaeI (771) DraIII (763) PshAI (746) BsaI (734) PflMI (712) AccI (693) SmlI (677) BpuEI (662) BglI (582) PspFI (538) BseYI (534) MmeI (525) NmeAIII (511) PfoI * (447) BpmI (431) BbsI (414) Bsu36I (372) PasI (337) TsoI (281) BsiHKAI (197) ApaLI (193) NspI (145) EarI - SapI - BspQI (117) AleI (107) BtgI - StyI - NcoI (105) BamHI (95) EciI (88) BsgI (47) AclI (44) Start (0) TagRFP-T P2A Kozak sequence pLenti-L3US2-RFP-HDAC3 931 bp

Sequence View

AclIBsgIStart (0) 60 PHPLVTYLHLLRRSREKECR g a g g g t g g g g g a g a a c c g t a t a t a a g t g c a g t a g t c g c c g t g a a c g t t c t t t t t c g c a a c c t c c c a c c c c c t c t t g g c a t a t a t t c a c g t c a t c a g c g g c a c t t g c a a g a a a a a g c g t t g

BspQISapIEarINcoIStyIBtgIAleIBamHIEciI 120 1 5 MVSKG TagRFP-T Kozak sequence MVSKG TQRWFVPTTVRSGGGHHRLA g g g t t t g c c g c c a g a a c a c a g g t g t c g t g a c g c g g g a t c c g c c a c c a t g g t g t c t a a g g g c c c a a a c g g c g g t c t t g t g t c c a c a g c a c t g c g c c c t a g g c g g t g g t a c c a c a g a t t c c c

NspI 180 10 15 20 25 EELIKENMHMKLYMEGTVNN TagRFP-T EELIKENMHMKLYMEGTVNN FLQNLLVHVHLQVHLAGHVV c g a a g a g c t g a t t a a g g a g a a c a t g c a c a t g a a g c t g t a c a t g g a g g g c a c c g t g a a c a a g c t t c t c g a c t a a t t c c t c t t g t a c g t g t a c t t c g a c a t g t a c c t c c c g t g g c a c t t g t t

ApaLIBsiHKAI 240 30 35 40 45 HHFKCTSEGEGKPYEGTQTM TagRFP-T HHFKCTSEGEGKPYEGTQTM VVELACGLAFALGVLAGLGH c c a c c a c t t c a a g t g c a c a t c c g a g g g c g a a g g c a a g c c c t a c g a g g g c a c c c a g a c c a t g g t g g t g a a g t t c a c g t g t a g g c t c c c g c t t c c g t t c g g g a t g c t c c c g t g g g t c t g g t a

TsoI 300 50 55 60 65 RIKVVEGGPLPFAFDILATS TagRFP-T RIKVVEGGPLPFAFDILATS SDLHDLAAREGEGEVDQSGA g a g a a t c a a g g t g g t c g a g g g c g g c c c t c t c c c c t t c g c c t t c g a c a t c c t g g c t a c c a g c t c t t a g t t c c a c c a g c t c c c g c c g g g a g a g g g g a a g c g g a a g c t g t a g g a c c g a t g g t c

PasI 360 70 75 80 85 FMYGSRTFINHTQGIPDFFK TagRFP-T FMYGSRTFINHTQGIPDFFK EHVAASGEDVVGLADGIEKL c t t c a t g t a c g g c a g c a g a a c c t t c a t c a a c c a c a c c c a g g g c a t c c c c g a t t t c t t t a a g a a g t a c a t g c c g t c g t c t t g g a a g t a g t t g g t g t g g g t c c c g t a g g g g c t a a a g a a a t t

BbsIBsu36I 420 90 95 100 105 QSFPEGFTWERVTTYEDGGV TagRFP-T QSFPEGFTWERVTTYEDGGV LGERLAECPLSDGCVFVPAH g c a g t c c t t c c c t g a g g g c t t c a c a t g g g a g a g a g t c a c c a c a t a c g a a g a c g g g g g c g t c g t c a g g a a g g g a c t c c c g a a g t g t a c c c t c t c t c a g t g g t g t a t g c t t c t g c c c c c g c a

PfoI*BpmI 480 110 115 120 125 LTATQDTSLQDGCLIYNVKI TagRFP-T LTATQDTSLQDGCLIYNVKI QGSGLVGAELVAAEDVVDLD g c t g a c c g c t a c c c a g g a c a c c a g c c t c c a g g a c g g c t g c c t c a t c t a c a a c g t c a a g a t c g a c t g g c g a t g g g t c c t g t g g t c g g a g g t c c t g c c g a c g g a g t a g a t g t t g c a g t t c t a

BseYIPspFIMmeINmeAIII 540 130 135 140 145 RGVNFPSNGPVMQKKTLGWE TagRFP-T RGVNFPSNGPVMQKKTLGWE SPHVEWGVARHHLLFCEAPL c a g a g g g g t g a a c t t c c c a t c c a a c g g c c c t g t g a t g c a g a a g a a a a c a c t c g g c t g g g a g t c t c c c c a c t t g a a g g g t a g g t t g c c g g g a c a c t a c g t c t t c t t t t g t g a g c c g a c c c t

BglI 600 150 155 160 165 ANTEMLYPADGGLEGRTDMA TagRFP-T ANTEMLYPADGGLEGRTDMA GVGLHQVGSVAAQFASGVHG g g c c a a c a c c g a g a t g c t g t a c c c c g c t g a c g g c g g c c t g g a a g g c a g a a c c g a c a t g g c c c g g t t g t g g c t c t a c g a c a t g g g g c g a c t g c c g c c g g a c c t t c c g t c t t g g c t g t a c c g

660 170 175 180 185 LKLVGGGHLICNFKTTYRSK TagRFP-T LKLVGGGHLICNFKTTYRSK QLEHAPAVQDAVELGCVSGL c c t g a a g c t c g t g g g c g g g g g c c a c c t g a t c t g c a a c t t c a a g a c c a c a t a c a g a t c c a a g g a c t t c g a g c a c c c g c c c c c g g t g g a c t a g a c g t t g a a g t t c t g g t g t a t g t c t a g g t t

PflMIAccISmlIBpuEI 720 190 195 200 205 KPAKNLKMPGVYYVDHRLER TagRFP-T KPAKNLKMPGVYYVDHRLER FGSLVELHGADVIHVVSQFS g a a a c c c g c t a a g a a c c t c a a g a t g c c c g g c g t c t a c t a t g t g g a c c a c a g a c t g g a a a g c t t t g g g c g a t t c t t g g a g t t c t a c g g g c c g c a g a t g a t a c a c c t g g t g t c t g a c c t t t c

AlwNIEaeIMscIDraIIIPshAIBsaI 780 210 215 220 225 IKEADKETYVEQHEVAVARY TagRFP-T IKEADKETYVEQHEVAVARY DLLGVFLGVDLLVLHSHGSV a a t c a a g g a g g c c g a c a a a g a g a c c t a c g t c g a g c a g c a c g a g g t g g c t g t g g c c a g a t a t t a g t t c c t c c g g c t g t t t c t c t g g a t g c a g c t c g t c g t g c t c c a c c g a c a c c g g t c t a t

NheIBmrI 840 230 235 240 CDLPSKLGHKLNGMDELYK A TagRFP-T CDLPSKLGHKLNGMDELYKA AVERAFQPVFKIAHVLQVLS c t g c g a c c t c c c t a g c a a a c t g g g g c a c a a a c t t a a t g g c a t g g a c g a g c t g t a c a a g g c g a c g c t g g a g g g a t c g t t t g a c c c c g t g t t t g a a t t a c c g t a c c t g c t c g a c a t g t t c c g

BmgBINgoMIVBsrFINaeIKasINarISfoIHaeIIPluTIBmtI 900 SGSG 1 5 10 15 ATNFSLLKQAGDVEEN P2A SGSGATNFSLLKQAGDVEEN AAAAGGVEAQQLLGAVHLLV t a g c g g c a g c g g c g c c a c c a a c t t c a g c c t g c t g a a g c a g g c c g g c g a c g t g g a g g a g a a a t c g c c g t c g c c g c g g t g g t t g a a g t c g g a c g a c t t c g t c c g g c c g c t g c a c c t c c t c t t

TaqIIEnd (931) BseRI 931 PGP (in frame with P2A) MTEYKPT P2A T A C T G G C T C A T G T T C G G G T G Puro-R PGPMTEYKPT GAGHGLVLGR c c c c g g c c c c a t g a c c g a g t a c a a g c c c a c g g g g g c c g g g g t a c t g g c t c a t g t t c g g g t g c

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.