Addgene pDONR223_ZBTB24_p.K123M Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: pDONR223_ZBTB24_p.K123M Sequencing Result


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PciI (4881) DrdI (4779) pBR322ori-F (4726 .. 4745) BssSI - BssSαI (4708) PaeR7I - PspXI - XhoI (4147) BssHII (4075) NaeI (4002) NgoMIV (4000) PvuI (3732) BstEII (3511) XbaI (2994) M13 Reverse (2906 .. 2922) M13 rev T7 (2882 .. 2901) EcoRV (2878) ScaI (2659) SacI (2643) L4440 (92 .. 109) BspQI - SapI (111) rrnB T2 terminator AclI (364) HincII - HpaI (501) M13/pUC Forward (522 .. 544) M13 Forward (536 .. 553) AhdI (549) AflII (554) CsiI - SexAI * (1029) Bsu36I (1545) EagI (1712) PshAI (1795) AleI (1956) SphI (2222) Eco53kI (2641) pDONR223_ZBTB24_p.K123M 4887 bp

Sequence View

5ʹ 3ʹ 60 c t t t c c t g c g t t a t c c c c t g a t t c t g t g g a t a a c c g t a t t a c c g c c t t t g a g t g a g c t g a g a a a g g a c g c a a t a g g g g a c t a a g a c a c c t a t t g g c a t a a t g g c g g a a a c t c a c t c g a c t

BspQISapI 120 A G C G A G T C A G T G A G C G A G L4440 t a c c g c t c g c c g c a g c c g a a c g a c c g a g c g c a g c g a g t c a g t g a g c g a g g a a g c g g a a g a a t g g c g a g c g g c g t c g g c t t g c t g g c t c g c g t c g c t c a g t c a c t c g c t c c t t c g c c t t c t

180 g c g c c c a a t a c g c a a a c c g c c t c t c c c c g c g c g t t g g c c g a t t c a t t a a t g c a g c t g g c a c g c g g g t t a t g c g t t t g g c g g a g a g g g g c g c g c a a c c g g c t a a g t a a t t a c g t c g a c c g t

240 c g a c a g g t t t c c c g a c t g g a a a g c g g g c a g t g a g c g c a a c g c a a t t a a t a c g c g t a c c g c g c t g t c c a a a g g g c t g a c c t t t c g c c c g t c a c t c g c g t t g c g t t a a t t a t g c g c a t g g c g

300 rrnB T2 terminator t a g c c a g g a a g a g t t t g t a g a a a c g c a a a a a g g c c a t c c g t c a g g a t g g c c t t c t g c t t a a t c g g t c c t t c t c a a a c a t c t t t g c g t t t t t c c g g t a g g c a g t c c t a c c g g a a g a c g a a t

360 g t t t g a t g c c t g g c a g t t t a t g g c g g g c g t c c t g c c c g c c a c c c t c c g g g c c g t t g c t t c c a a a c t a c g g a c c g t c a a a t a c c g c c c g c a g g a c g g g c g g t g g g a g g c c c g g c a a c g a a g

AclI 420 rrnB T1 terminator a c a a c g t t c a a a t c c g c t c c c g g c g g a t t t g t c c t a c t c a g g a g a g c g t t c a c c g a c a a a t g t t g c a a g t t t a g g c g a g g g c c g c c t a a a c a g g a t g a g t c c t c t c g c a a g t g g c t g t t t

480 rrnB T1 terminator c a a c a g a t a a a a c g a a a g g c c c a g t c t t c c g a c t g a g c c t t t c g t t t t a t t t g a t g c c t g g t t g t c t a t t t t g c t t t c c g g g t c a g a a g g c t g a c t c g g a a a g c a a a a t a a a c t a c g g a c

HincIIHpaI 540 M13 fwd C C C A G T C A C G A C G T T G T A A M13/pUC Forward T G T A A M13 Forward g c a g t t c c c t a c t c t c g c g t t a a c g c t a g c a t g g a t g t t t t c c c a g t c a c g a c g t t g t a a c g t c a a g g g a t g a g a g c g c a a t t g c g a t c g t a c c t a c a a a a g g g t c a g t g c t g c a a c a t t

AflIIAhdI 600 M13 fwd attL1 A A C G M13/pUC Forward A A C G A C G G C C A G T M13 Forward a a c g a c g g c c a g t c t t a a g c t c g g g c c c c a a a t a a t g a t t t t a t t t t g a c t g a t a g t g a c t t g c t g c c g g t c a g a a t t c g a g c c c g g g g t t t a t t a c t a a a a t a a a a c t g a c t a t c a c t g

660 attL1 MPTLYKK c t g t t c g t t g c a a c a a a t t g a t g a g c a a t g c t t t t t t a t a a t g c c a a c t t t g t a c a a a a a g a c a a g c a a c g t t g t t t a a c t a c t c g t t a c g a a a a a a t a t t a c g g t t g a a a c a t g t t t t t

720 attL1 VGTMAETSPEPSGQLVVHSD a g t t g g c a c c a t g g c a g a a a c a t c g c c a g a g c c t t c t g g g c a g c t t g t t g t a c a c t c a g a t c a a c c g t g g t a c c g t c t t t g t a g c g g t c t c g g a a g a c c c g t c g a a c a a c a t g t g a g t c t

780 AHSDTVLASFEDQRKKGFLC c g c t c a c a g t g a c a c t g t g c t g g c c a g t t t t g a g g a t c a g a g g a a g a a a g g c t t c c t c t g g c g a g t g t c a c t g t g a c a c g a c c g g t c a a a a c t c c t a g t c t c c t t c t t t c c g a a g g a g a c

840 DITLIVENVHFRAHKALLAA t g a c a t t a c t t t a a t c g t g g a g a a t g t a c a t t t c c g g g c c c a c a a a g c c t t a c t t g c t g c a c t g t a a t g a a a t t a g c a c c t c t t a c a t g t a a a g g c c c g g g t g t t t c g g a a t g a a c g a c g

900 SSEYFSMMFAEEGEIGQSIY c a g t a g t g a a t a c t t c t c a a t g a t g t t t g c a g a a g a g g g g g a a a t c g g c c a a t c c a t t t a g t c a t c a c t t a t g a a g a g t t a c t a c a a a c g t c t t c t c c c c c t t t a g c c g g t t a g g t a a a t

960 MLEGMVADTFGILLEFIYTG t a t g c t g g a a g g c a t g g t t g c a g a c a c c t t t g g t a t c c t g c t g g a a t t t a t c t a c a c a g g a t a c g a c c t t c c g t a c c a a c g t c t g t g g a a a c c a t a g g a c g a c c t t a a a t a g a t g t g t c c

1020 YLHASEKSTEQILATAQFLK t t a t c t c c a t g c c a g t g a g a a a a g t a c a g a a c a a a t c c t g g c t a c t g c t c a g t t c t t a a a a a t a g a g g t a c g g t c a c t c t t t t c a t g t c t t g t t t a g g a c c g a t g a c g a g t c a a g a a t t t

SexAI*CsiI 1080 VYDLVMAYTDFQNNHSSPKP a g t c t a t g a c c t g g t a a t g g c t t a c a c a g a c t t c c a a a a t a a t c a t a g c t c c c c a a a g c c t c a g a t a c t g g a c c a t t a c c g a a t g t g t c t g a a g g t t t t a t t a g t a t c g a g g g g t t t c g g

1140 TTLNTAGAPVVVISNKKNDP a a c a a c t t t g a a c a c t g c t g g t g c c c c a g t g g t t g t t a t c t c t a a t a a g a a a a a c g a t c c t t g t t g a a a c t t g t g a c g a c c a c g g g g t c a c c a a c a a t a g a g a t t a t t c t t t t t g c t a g g

1200 PKRKRGRPKKVNTLQEEKSE t c c a a a g c g g a a a c g g g g a a g a c c a a a a a a a g t c a a t a c a t t g c a g g a g g a g a a a t c a g a a g g t t t c g c c t t t g c c c c t t c t g g t t t t t t t c a g t t a t g t a a c g t c c t c c t c t t t a g t c t

1260 LAAEEEIQLRVNNSVQNRQN a c t g g c t g c a g a g g a a g a a a t a c a g t t a a g a g t g a a c a a t t c a g t t c a g a a t a g a c a a a a t g a c c g a c g t c t c c t t c t t t a t g t c a a t t c t c a c t t g t t a a g t c a a g t c t t a t c t g t t t t

1320 FVVKGDSGVLNEQIAAKEKE c t t t g t g g t t a a a g g a g a c a g t g g t g t a c t g a a t g a g c a a a t t g c a g c a a a a g a a a a g g a g a a a c a c c a a t t t c c t c t g t c a c c a c a t g a c t t a c t c g t t t a a c g t c g t t t t c t t t t c c t

1380 ESEPTCEPSREEEMPVEKDE a g a a t c g g a g c c t a c t t g t g a g c c a a g t a g a g a g g a g g a a a t g c c a g t t g a a a a a g a t g a t c t t a g c c t c g g a t g a a c a c t c g g t t c a t c t c t c c t c c t t t a c g g t c a a c t t t t t c t a c t

1440 NYDPKTEDGQASQSRYSKRR g a a c t a t g a t c c c a a g a c c g a g g a t g g c c a g g c a a g c c a g a g t c g a t a c a g c a a g c g g a g c t t g a t a c t a g g g t t c t g g c t c c t a c c g g t c c g t t c g g t c t c a g c t a t g t c g t t c g c c t c

1500 IWRSVKLKDYKLVGDQEDHG g a t t t g g a g a t c c g t c a a a c t t a a a g a t t a c a a a c t t g t t g g g g a t c a a g a g g a c c a t g g c t a a a c c t c t a g g c a g t t t g a a t t t c t a a t g t t t g a a c a a c c c c t a g t t c t c c t g g t a c c

Bsu36I 1560 SAKRICGRRKRPGGPEARCK t t c a g c c a a g a g g a t c t g t g g a a g g a g a a a g c g c c c t g g a g g c c c t g a g g c c c g c t g t a a a a g t c g g t t c t c c t a g a c a c c t t c c t c t t t c g c g g g a c c t c c g g g a c t c c g g g c g a c a t t

1620 DCGKVFKYNHFLAIHQRSHT a g a c t g t g g c a a g g t c t t t a a g t a c a a t c a c t t t t t a g c a a t c c a c c a g a g g a g c c a c a c t c t g a c a c c g t t c c a g a a a t t c a t g t t a g t g a a a a a t c g t t a g g t g g t c t c c t c g g t g t g

1680 GERPFKCNECGKGFAQKHSL a g g g g a g c g a c c t t t c a a a t g t a a t g a g t g t g g a a a a g g c t t t g c c c a g a a g c a c t c g c t t c c c c t c g c t g g a a a g t t t a c a t t a c t c a c a c c t t t t c c g a a a c g g g t c t t c g t g a g c g a

EagI 1740 QVHTRMHTGERPYTCTVCSK a c a g g t c c a c a c c a g g a t g c a c a c a g g c g a g c g g c c g t a c a c c t g c a c c g t g t g c a g c a a t g t c c a g g t g t g g t c c t a c g t g t g t c c g c t c g c c g g c a t g t g g a c g t g g c a c a c g t c g t t

PshAI 1800 ALTTKHSLLEHMSLHSGQKS g g c t c t g a c c a c c a a g c a c t c a c t g c t g g a g c a c a t g a g c c t g c a c t c a g g a c a g a a g t c c c g a g a c t g g t g g t t c g t g a g t g a c g a c c t c g t g t a c t c g g a c g t g a g t c c t g t c t t c a g

1860 FTCDQCGKYFSQNRQLKSHY t t t t a c c t g t g a t c a a t g c g g a a a a t a t t t c a g c c a g a a c a g a c a g c t a a a g a g c c a t t a a a a a t g g a c a c t a g t t a c g c c t t t t a t a a a g t c g g t c t t g t c t g t c g a t t t c t c g g t a a t

1920 RVHTGHSLPECKDCHRKFMD c c g a g t t c a t a c a g g c c a c t c a t t a c c g g a a t g c a a a g a c t g c c a t c g c a a a t t c a t g g a g g c t c a a g t a t g t c c g g t g a g t a a t g g c c t t a c g t t t c t g a c g g t a g c g t t t a a g t a c c t

AleI 1980 VSQLKKHLRTHTGEKPFTCE t g t g t c t c a g c t a a a g a a a c a t c t g c g a a c a c a c a c a g g t g a g a a g c c a t t t a c t t g t g a a c a c a g a g t c g a t t t c t t t g t a g a c g c t t g t g t g t g t c c a c t c t t c g g t a a a t g a a c a c t

2040 ICGKSFTAKSSLQTHIRIHR a a t c t g t g g c a a a t c t t t c a c a g c a a a g a g t t c t c t t c a g a c c c a c a t c a g a a t c c a t c g t t a g a c a c c g t t t a g a a a g t g t c g t t t c t c a a g a g a a g t c t g g g t g t a g t c t t a g g t a g c

2100 GEKPYSCGICGKSFSDSSAK a g g a g a a a a g c c a t a c t c c t g t g g c a t t t g t g g c a a a t c c t t c t c t g a c t c c a g t g c c a a t c c t c t t t t c g g t a t g a g g a c a c c g t a a a c a c c g t t t a g g a a g a g a c t g a g g t c a c g g t t

2160 RRHCILHTGKKPFSCPECNL a a g g a g a c a c t g c a t t c t a c a c a c t g g c a a g a a g c c t t t c t c c t g c c c t g a g t g t a a c t t t t c c t c t g t g a c g t a a g a t g t g t g a c c g t t c t t c g g a a a g a g g a c g g g a c t c a c a t t g a a

2220 QFARLDNLKAHLKIHSKEKH a c a g t t t g c t c g c t t a g a c a a c t t g a a g g c t c a c t t g a a a a t t c a t a g c a a g g a g a a g c a t g t c a a a c g a g c g a a t c t g t t g a a c t t c c g a g t g a a c t t t t a a g t a t c g t t c c t c t t c g t

SphI 2280 ASDASSISGSSNTEEVRNIL t g c t t c a g a t g c c a g c a g c a t t t c t g g c a g t a g t a a t a c a g a a g a g g t c a g g a a t a t t c t a c g a a g t c t a c g g t c g t c g t a a a g a c c g t c a t c a t t a t g t c t t c t c c a g t c c t t a t a a g a

2340 QLQPYQLSTSGEQEIQLLVT t c a g c t a c a g c c a t a t c a a c t c t c t a c c t c g g g a g a g c a g g a a a t t c a g c t t c t c g t a a c a g t c g a t g t c g g t a t a g t t g a g a g a t g g a g c c c t c t c g t c c t t t a a g t c g a a g a g c a t t g

2400 DSVHNINFMPGPSQGISIVT c g a t t c t g t a c a t a a c a t c a a t t t c a t g c c c g g t c c t a g c c a g g g a a t c a g c a t t g t g a c g c t a a g a c a t g t a t t g t a g t t a a a g t a c g g g c c a g g a t c g g t c c c t t a g t c g t a a c a c t g

2460 AESSQNMTADQAANLTLLTQ t g c a g a g a g t t c c c a a a a c a t g a c t g c a g a c c a g g c t g c t a a t c t t a c c c t g c t c a c g c a a c g t c t c t c a a g g g t t t t g t a c t g a c g t c t g g t c c g a c g a t t a g a a t g g g a c g a g t g c g t

2520 QPEQLQNLILSAQQEQTEHI g c a g c c a g a g c a a c t g c a g a a t t t a a t t c t t t c a g c t c a a c a g g a g c a a a c a g a a c a c a t c g t c g g t c t c g t t g a c g t c t t a a a t t a a g a a a g t c g a g t t g t c c t c g t t t g t c t t g t g t a

2580 QSLNMIESQMGPSQTEPVHV t c a g a g c c t c a a t a t g a t t g a a a g c c a g a t g g g g c c c t c a c a a a c a g a g c c a g t g c a c g t a g t c t c g g a g t t a t a c t a a c t t t c g g t c t a c c c c g g g a g t g t t t g t c t c g g t c a c g t g c a

2640 ITLSKETLEHLHAHQEQTEE g a t c a c t c t g t c c a a g g a a a c a c t g g a a c a t c t t c a t g c c c a t c a a g a g c a a a c a g a g g a c t a g t g a g a c a g g t t c c t t t g t g a c c t t g t a g a a g t a c g g g t a g t t c t c g t t t g t c t c c t

ScaIEco53kISacI 2700 LHLATSTSDPAQHLQLTQEP g c t c c a t t t a g c t a c a a g t a c t t c a g a t c c a g c t c a g c a c c t g c a g c t g a c a c a g g a g c c c g a g g t a a a t c g a t g t t c a t g a a g t c t a g g t c g a g t c g t g g a c g t c g a c t g t g t c c t c g g

2760 GPPPPTHHVPQPTPLGQEQS t g g t c c g c c a c c a c c c a c t c a c c a c g t g c c c c a g c c a a c g c c a c t t g g c c a g g a g c a g a g a c c a g g c g g t g g t g g g t g a g t g g t g c a c g g g g t c g g t t g c g g t g a a c c g g t c c t c g t c t c

2820 attL2 * c t g a t t g c c a a c t t t c t t g t a c a a a g t t g g c a t t a t a a g a a a g c a t t g c t t a t c a a t t t g g a c t a a c g g t t g a a a g a a c a t g t t t c a a c c g t a a t a t t c t t t c g t a a c g a a t a g t t a a a c

EcoRV 2880 attL2 t t g c a a c g a a c a g g t c a c t a t c a g t c a a a a t a a a a t c a t t a t t t g c c a t c c a g c t g a t a t a a c g t t g c t t g t c c a g t g a t a g t c a g t t t t a t t t t a g t a a t a a a c g g t a g g t c g a c t a t a

2940 T7 promoter M13 rev G G G A T A T C A C T C A G C A T A A T T7 C A G T A T C G A C A A A G G A C M13 Reverse c c c c t a t a g t g a g t c g t a t t a c a t g g t c a t a g c t g t t t c c t g g c a g c t c t g g c c c g t g t c g g g g a t a t c a c t c a g c a t a a t g t a c c a g t a t c g a c a a a g g a c c g t c g a g a c c g g g c a c a g

XbaI 3000 t c a a a a t c t c t g a t g t t a c a t t g c a c a a g a t a a a a a t a t a t c a t c a t g c c t c c t c t a g a c a g t t t t a g a g a c t a c a a t g t a a c g t g t t c t a t t t t t a t a t a g t a g t a c g g a g g a g a t c t g

3060 c a g c c a g g a c a g a a a t g c c t c g a c t t c g c t g c t a c c c a a g g t t g c c g g g t g a c g c a c a c c g t c g g t c c t g t c t t t a c g g a g c t g a a g c g a c g a t g g g t t c c a a c g g c c c a c t g c g t g t g g

3120 g t g g a a a c g g a t g a a g g c a c g a a c c c a g t g g a c a t a a g c c t g t t c g g t t c g t a a g c t g t a c a c c t t t g c c t a c t t c c g t g c t t g g g t c a c c t g t a t t c g g a c a a g c c a a g c a t t c g a c a t

3180 MRSRNWSRTLTERSGG a t g c a a g t a g c g t a t g c g c t c a c g c a a c t g g t c c a g a a c c t t g a c c g a a c g c a g c g g t g g t a c g t t c a t c g c a t a c g c g a g t g c g t t g a c c a g g t c t t g g a a c t g g c t t g c g t c g c c a c c

3240 NGAVAVFMACYDCFFGVQSM t a a c g g c g c a g t g g c g g t t t t c a t g g c t t g t t a t g a c t g t t t t t t t g g g g t a c a g t c t a t a t t g c c g c g t c a c c g c c a a a a g t a c c g a a c a a t a c t g a c a a a a a a a c c c c a t g t c a g a t a

3300 PRASKQQARYAVGRCLMLWS g c c t c g g g c a t c c a a g c a g c a a g c g c g t t a c g c c g t g g g t c g a t g t t t g a t g t t a t g g a g c g g a g c c c g t a g g t t c g t c g t t c g c g c a a t g c g g c a c c c a g c t a c a a a c t a c a a t a c c t c

3360 1 MRE SmR SNDVTQQGSRPKTKLNIMRE c a g c a a c g a t g t t a c g c a g c a g g g c a g t c g c c c t a a a a c a a a g t t a a a c a t t a t g a g g g a g t c g t t g c t a c a a t g c g t c g t c c c g t c a g c g g g a t t t t g t t t c a a t t t g t a a t a c t c c c t

3420 5 10 15 20 AVIAEVSTQLSEVVGVIERH SmR AVIAEVSTQLSEVVGVIERH a g c g g t g a t c g c c g a a g t a t c g a c t c a a c t a t c a g a g g t a g t t g g c g t c a t c g a g c g c c a t c g c c a c t a g c g g c t t c a t a g c t g a g t t g a t a g t c t c c a t c a a c c g c a g t a g c t c g c g g t

3480 25 30 35 40 LEPTLLAVHLYGSAVDGGLK SmR LEPTLLAVHLYGSAVDGGLK t c t c g a a c c g a c g t t g c t g g c c g t a c a t t t g t a c g g c t c c g c a g t g g a t g g c g g c c t g a a a g a g c t t g g c t g c a a c g a c c g g c a t g t a a a c a t g c c g a g g c g t c a c c t a c c g c c g g a c t t

BstEII 3540 45 50 55 60 PHSDIDLLVTVTVRLDETTR SmR PHSDIDLLVTVTVRLDETTR g c c a c a c a g t g a t a t t g a t t t g c t g g t t a c g g t g a c c g t a a g g c t t g a t g a a a c a a c g c g c g g t g t g t c a c t a t a a c t a a a c g a c c a a t g c c a c t g g c a t t c c g a a c t a c t t t g t t g c g c

3600 65 70 75 80 RALINDLLETSASPGESEIL SmR RALINDLLETSASPGESEIL g c g a g c t t t g a t c a a c g a c c t t t t g g a a a c t t c g g c t t c c c c t g g a g a g a g c g a g a t t c t c g c t c g a a a c t a g t t g c t g g a a a a c c t t t g a a g c c g a a g g g g a c c t c t c t c g c t c t a a g a

3660 85 90 95 100 RAVEVTIVVHDDIIPWRYPA SmR RAVEVTIVVHDDIIPWRYPA c c g c g c t g t a g a a g t c a c c a t t g t t g t g c a c g a c g a c a t c a t t c c g t g g c g t t a t c c a g c g g c g c g a c a t c t t c a g t g g t a a c a a c a c g t g c t g c t g t a g t a a g g c a c c g c a a t a g g t c g

3720 105 110 115 120 KRELQFGEWQRNDILAGIFE SmR KRELQFGEWQRNDILAGIFE t a a g c g c g a a c t g c a a t t t g g a g a a t g g c a g c g c a a t g a c a t t c t t g c a g g t a t c t t c g a a t t c g c g c t t g a c g t t a a a c c t c t t a c c g t c g c g t t a c t g t a a g a a c g t c c a t a g a a g c t

PvuI 3780 125 130 135 140 PATIDIDLAILLTKAREHSV SmR PATIDIDLAILLTKAREHSV g c c a g c c a c g a t c g a c a t t g a t c t g g c t a t c t t g c t g a c a a a a g c a a g a g a a c a t a g c g t c g g t c g g t g c t a g c t g t a a c t a g a c c g a t a g a a c g a c t g t t t t c g t t c t c t t g t a t c g c a

3840 145 150 155 160 ALVGPAAEELFDPVPEQDLF SmR ALVGPAAEELFDPVPEQDLF t g c c t t g g t a g g t c c a g c g g c g g a g g a a c t c t t t g a t c c g g t t c c t g a a c a g g a t c t a t t a c g g a a c c a t c c a g g t c g c c g c c t c c t t g a g a a a c t a g g c c a a g g a c t t g t c c t a g a t a a

3900 165 170 175 180 EALNETLTLWNSPPDWAGDE SmR EALNETLTLWNSPPDWAGDE t g a g g c g c t a a a t g a a a c c t t a a c g c t a t g g a a c t c g c c g c c c g a c t g g g c t g g c g a t g a a c t c c g c g a t t t a c t t t g g a a t t g c g a t a c c t t g a g c g g c g g g c t g a c c c g a c c g c t a c t

3960 185 190 195 200 RNVVLTLSRIWYSAVTGKIA SmR RNVVLTLSRIWYSAVTGKIA g c g a a a t g t a g t g c t t a c g t t g t c c c g c a t t t g g t a c a g c g c a g t a a c c g g c a a a a t c g c c g c t t t a c a t c a c g a a t g c a a c a g g g c g t a a a c c a t g t c g c g t c a t t g g c c g t t t t a g c g

NgoMIVNaeI 4020 205 210 215 220 PKDVAADWAMERLPAQYQPV SmR PKDVAADWAMERLPAQYQPV g c c g a a g g a t g t c g c t g c c g a c t g g g c a a t g g a g c g c c t g c c g g c c c a g t a t c a g c c c g t c g g c t t c c t a c a g c g a c g g c t g a c c c g t t a c c t c g c g g a c g g c c g g g t c a t a g t c g g g c a

BssHII 4080 225 230 235 240 ILEARQAYLGQEEDRLASRA SmR ILEARQAYLGQEEDRLASRA c a t a c t t g a a g c t a g a c a g g c t t a t c t t g g a c a a g a a g a a g a t c g c t t g g c c t c g c g c g c g t a t g a a c t t c g a t c t g t c c g a a t a g a a c c t g t t c t t c t t c t a g c g a a c c g g a g c g c g c g

4140 245 250 255 260 DQLEEFVHYVKGEITKVVGK SmR DQLEEFVHYVKGEITKVVGK a g a t c a g t t g g a a g a a t t t g t c c a c t a c g t g a a a g g c g a g a t c a c c a a g g t a g t c g g c a a t c t a g t c a a c c t t c t t a a a c a g g t g a t g c a c t t t c c g c t c t a g t g g t t c c a t c a g c c g t t

PaeR7IPspXIXhoI 4200 * SmR * a t a a c c c t c g a g c c a c c c a t g a c c a a a a t c c c t t a a c g t g a g t t a c g c g t c g t t c c a c t g t a t t g g g a g c t c g g t g g g t a c t g g t t t t a g g g a a t t g c a c t c a a t g c g c a g c a a g g t g a c

4260 ori a g c g t c a g a c c c c g t a g a a a a g a t c a a a g g a t c t t c t t g a g a t c c t t t t t t t c t g c g c g t t c g c a g t c t g g g g c a t c t t t t c t a g t t t c c t a g a a g a a c t c t a g g a a a a a a a g a c g c g c a

4320 ori a a t c t g c t g c t t g c a a a c a a a a a a a c c a c c g c t a c c a g c g g t g g t t t g t t t g c c g g a t c a t t a g a c g a c g a a c g t t t g t t t t t t t g g t g g c g a t g g t c g c c a c c a a a c a a a c g g c c t a g t

4380 ori a g a g c t a c c a a c t c t t t t t c c g a a g g t a a c t g g c t t c a g c a g a g c g c a g a t a c c a a a t a c t c t c g a t g g t t g a g a a a a a g g c t t c c a t t g a c c g a a g t c g t c t c g c g t c t a t g g t t t a t g

4440 ori t g t t c t t c t a g t g t a g c c g t a g t t a g g c c a c c a c t t c a a g a a c t c t g t a g c a c c g c c t a c a c a a g a a g a t c a c a t c g g c a t c a a t c c g g t g g t g a a g t t c t t g a g a c a t c g t g g c g g a t g

4500 ori a t a c c t c g c t c t g c t a a t c c t g t t a c c a g t g g c t g c t g c c a g t g g c g a t a a g t c g t g t c t t a t g g a g c g a g a c g a t t a g g a c a a t g g t c a c c g a c g a c g g t c a c c g c t a t t c a g c a c a g a

4560 ori t a c c g g g t t g g a c t c a a g a c g a t a g t t a c c g g a t a a g g c g c a g c g g t c g g g c t g a a c g g g a t g g c c c a a c c t g a g t t c t g c t a t c a a t g g c c t a t t c c g c g t c g c c a g c c c g a c t t g c c c

4620 ori g g g t t c g t g c a c a c a g c c c a g c t t g g a g c g a a c g a c c t a c a c c g a a c t g a g a t a c c t a c a c c c a a g c a c g t g t g t c g g g t c g a a c c t c g c t t g c t g g a t g t g g c t t g a c t c t a t g g a t g t

4680 ori g c g t g a g c t a t g a g a a a g c g c c a c g c t t c c c g a a g g g a g a a a g g c g g a c a g g t a t c c g g t c g c a c t c g a t a c t c t t t c g c g g t g c g a a g g g c t t c c c t c t t t c c g c c t g t c c a t a g g c c a

BssSαIBssSI 4740 ori G G G A A A C G C C T G G T A pBR322ori-F a a g c g g c a g g g t c g g a a c a g g a g a g c g c a c g a g g g a g c t t c c a g g g g g a a a c g c c t g g t a t t c g c c g t c c c a g c c t t g t c c t c t c g c g t g c t c c c t c g a a g g t c c c c c t t t g c g g a c c a t

DrdI 4800 ori T C T T T pBR322ori-F t c t t t a t a g t c c t g t c g g g t t t c g c c a c c t c t g a c t t g a g c g t c g a t t t t t g t g a t g c t c a g a a a t a t c a g g a c a g c c c a a a g c g g t g g a g a c t g a a c t c g c a g c t a a a a a c a c t a c g a g

4860 ori g t c a g g g g g g c g g a g c c t a t g g a a a a a c g c c a g c a a c g c g g c c t t t t t a c g g t t c c t g g c c a g t c c c c c c g c c t c g g a t a c c t t t t t g c g g t c g t t g c g c c g g a a a a a t g c c a a g g a c c g

PciI 3ʹ 5ʹ 4887 c t t t t g c t g g c c t t t t g c t c a c a t g t t g a a a a c g a c c g g a a a a c g a g t g t a c a a

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.