Addgene pDONR223_TP53_p.P278H Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pDONR223_TP53_p.P278H Sequencing Result


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pBR322ori-F (3811 .. 3830) BciVI (3768) PaeR7I - PspXI - XhoI (3232) BssHII (3160) NaeI (3087) NgoMIV (3085) BglI (3068) PvuI (2817) BlpI (2745) MslI (2708) BclI * (2634) BstEII (2596) XbaI (2079) M13 Reverse (1991 .. 2007) T7 (1967 .. 1986) L4440 (92 .. 109) BspQI - SapI (111) HincII - HpaI (501) M13/pUC Forward (522 .. 544) M13 Forward (536 .. 553) M13 fwd AhdI (549) AflII (554) PspOMI (563) ApaI (567) PshAI (840) SgrAI (910) NcoI (1143) BbvCI - Bpu10I (1239) Bsu36I (1335) BsaI (1500) PasI (1568) SspI (1646) StuI (1706) AccI (1793) EcoRV (1963) T7 promoter pDONR223_TP53_p.P278H 3972 bp

Sequence View

60 c t t t c c t g c g t t a t c c c c t g a t t c t g t g g a t a a c c g t a t t a c c g c c t t t g a g t g a g c t g a g a a a g g a c g c a a t a g g g g a c t a a g a c a c c t a t t g g c a t a a t g g c g g a a a c t c a c t c g a c t

BspQISapI 120 A G C G A G T C A G T G A G C G A G L4440 t a c c g c t c g c c g c a g c c g a a c g a c c g a g c g c a g c g a g t c a g t g a g c g a g g a a g c g g a a g a a t g g c g a g c g g c g t c g g c t t g c t g g c t c g c g t c g c t c a g t c a c t c g c t c c t t c g c c t t c t

180 g c g c c c a a t a c g c a a a c c g c c t c t c c c c g c g c g t t g g c c g a t t c a t t a a t g c a g c t g g c a c g c g g g t t a t g c g t t t g g c g g a g a g g g g c g c g c a a c c g g c t a a g t a a t t a c g t c g a c c g t

240 c g a c a g g t t t c c c g a c t g g a a a g c g g g c a g t g a g c g c a a c g c a a t t a a t a c g c g t a c c g c g c t g t c c a a a g g g c t g a c c t t t c g c c c g t c a c t c g c g t t g c g t t a a t t a t g c g c a t g g c g

300 rrnB T2 terminator t a g c c a g g a a g a g t t t g t a g a a a c g c a a a a a g g c c a t c c g t c a g g a t g g c c t t c t g c t t a a t c g g t c c t t c t c a a a c a t c t t t g c g t t t t t c c g g t a g g c a g t c c t a c c g g a a g a c g a a t

360 g t t t g a t g c c t g g c a g t t t a t g g c g g g c g t c c t g c c c g c c a c c c t c c g g g c c g t t g c t t c c a a a c t a c g g a c c g t c a a a t a c c g c c c g c a g g a c g g g c g g t g g g a g g c c c g g c a a c g a a g

420 rrnB T1 terminator a c a a c g t t c a a a t c c g c t c c c g g c g g a t t t g t c c t a c t c a g g a g a g c g t t c a c c g a c a a a t g t t g c a a g t t t a g g c g a g g g c c g c c t a a a c a g g a t g a g t c c t c t c g c a a g t g g c t g t t t

480 rrnB T1 terminator c a a c a g a t a a a a c g a a a g g c c c a g t c t t c c g a c t g a g c c t t t c g t t t t a t t t g a t g c c t g g t t g t c t a t t t t g c t t t c c g g g t c a g a a g g c t g a c t c g g a a a g c a a a a t a a a c t a c g g a c

HincIIHpaI 540 M13 fwd C C C A G T C A C G A C G T T G T A A M13/pUC Forward T G T A A M13 Forward g c a g t t c c c t a c t c t c g c g t t a a c g c t a g c a t g g a t g t t t t c c c a g t c a c g a c g t t g t a a c g t c a a g g g a t g a g a g c g c a a t t g c g a t c g t a c c t a c a a a a g g g t c a g t g c t g c a a c a t t

PspOMIApaIAflIIAhdI 600 M13 fwd attL1 A A C G M13/pUC Forward A A C G A C G G C C A G T M13 Forward a a c g a c g g c c a g t c t t a a g c t c g g g c c c c a a a t a a t g a t t t t a t t t t g a c t g a t a g t g a c t t g c t g c c g g t c a g a a t t c g a g c c c g g g g t t t a t t a c t a a a a t a a a a c t g a c t a t c a c t g

660 attL1 MPTLYKK c t g t t c g t t g c a a c a a a t t g a t g a g c a a t g c t t t t t t a t a a t g c c a a c t t t g t a c a a a a a g a c a a g c a a c g t t g t t t a a c t a c t c g t t a c g a a a a a a t a t t a c g g t t g a a a c a t g t t t t t

720 1 5 10 15 MEEPQSDPSVEPPLSQET p53 attL1 VGMEEPQSDPSVEPPLSQET a g t t g g c a t g g a g g a g c c g c a g t c a g a t c c t a g c g t c g a g c c c c c t c t g a g t c a g g a a a c t c a a c c g t a c c t c c t c g g c g t c a g t c t a g g a t c g c a g c t c g g g g g a g a c t c a g t c c t t t g

780 20 25 30 35 FSDLWKLLPENNVLSPLPSQ p53 FSDLWKLLPENNVLSPLPSQ a t t t t c a g a c c t a t g g a a a c t a c t t c c t g a a a a c a a c g t t c t g t c c c c c t t g c c g t c c c a t a a a a g t c t g g a t a c c t t t g a t g a a g g a c t t t t g t t g c a a g a c a g g g g g a a c g g c a g g g t

PshAI 840 40 45 50 55 AMDDLMLSPDDIEQWFTEDP p53 AMDDLMLSPDDIEQWFTEDP a g c a a t g g a t g a t t t g a t g c t g t c c c c g g a c g a t a t t g a a c a a t g g t t c a c t g a a g a c c c t c g t t a c c t a c t a a a c t a c g a c a g g g g c c t g c t a t a a c t t g t t a c c a a g t g a c t t c t g g g

900 60 65 70 75 GPDEAPRMPEAAPRVAPAPA p53 GPDEAPRMPEAAPRVAPAPA a g g t c c a g a t g a a g c t c c c a g a a t g c c a g a g g c t g c t c c c c g c g t g g c c c c t g c a c c a g c t c c a g g t c t a c t t c g a g g g t c t t a c g g t c t c c g a c g a g g g g c g c a c c g g g g a c g t g g t c g

SgrAI 960 80 85 90 95 APTPAAPAPAPSWPLSSSVP p53 APTPAAPAPAPSWPLSSSVP a g c t c c t a c a c c g g c g g c c c c t g c a c c a g c c c c c t c c t g g c c c c t g t c a t c t t c t g t c c c t c g a g g a t g t g g c c g c c g g g g a c g t g g t c g g g g g a g g a c c g g g g a c a g t a g a a g a c a g g g

1020 100 105 110 115 SQKTYQGSYGFRLGFLHSGT p53 SQKTYQGSYGFRLGFLHSGT t t c c c a g a a a a c c t a c c a g g g c a g c t a c g g t t t c c g t c t g g g c t t c t t g c a t t c t g g g a c a a g g g t c t t t t g g a t g g t c c c g t c g a t g c c a a a g g c a g a c c c g a a g a a c g t a a g a c c c t g

1080 120 125 130 135 AKSVTCTYSPALNKMFCQLA p53 AKSVTCTYSPALNKMFCQLA a g c c a a g t c t g t g a c t t g c a c g t a c t c c c c t g c c c t c a a c a a g a t g t t t t g c c a a c t g g c t c g g t t c a g a c a c t g a a c g t g c a t g a g g g g a c g g g a g t t g t t c t a c a a a a c g g t t g a c c g

1140 140 145 150 155 KTCPVQLWVDSTPPPGTRVR p53 KTCPVQLWVDSTPPPGTRVR c a a g a c c t g c c c t g t g c a g c t g t g g g t t g a t t c c a c a c c c c c g c c c g g c a c c c g c g t c c g g t t c t g g a c g g g a c a c g t c g a c a c c c a a c t a a g g t g t g g g g g c g g g c c g t g g g c g c a g g c

NcoI 1200 160 165 170 175 AMAIYKQSQHMTEVVRRCPH p53 AMAIYKQSQHMTEVVRRCPH c g c c a t g g c c a t c t a c a a g c a g t c a c a g c a c a t g a c g g a g g t t g t g a g g c g c t g c c c c c a g c g g t a c c g g t a g a t g t t c g t c a g t g t c g t g t a c t g c c t c c a a c a c t c c g c g a c g g g g g t

Bpu10IBbvCI 1260 180 185 190 195 HERCSDSDGLAPPQHLIRVE p53 HERCSDSDGLAPPQHLIRVE c c a t g a g c g c t g c t c a g a t a g c g a t g g t c t g g c c c c t c c t c a g c a t c t t a t c c g a g t g g a g g t a c t c g c g a c g a g t c t a t c g c t a c c a g a c c g g g g a g g a g t c g t a g a a t a g g c t c a c c t

1320 200 205 210 215 GNLRVEYLDDRNTFRHSVVV p53 GNLRVEYLDDRNTFRHSVVV a g g a a a t t t g c g t g t g g a g t a t t t g g a t g a c a g a a a c a c t t t t c g a c a t a g t g t g g t g g t t c c t t t a a a c g c a c a c c t c a t a a a c c t a c t g t c t t t g t g a a a a g c t g t a t c a c a c c a c c a

Bsu36I 1380 220 225 230 235 PYEPPEVGSDCTTIHYNYMC p53 PYEPPEVGSDCTTIHYNYMC g c c c t a t g a g c c g c c t g a g g t t g g c t c t g a c t g t a c c a c c a t c c a c t a c a a c t a c a t g t g c g g g a t a c t c g g c g g a c t c c a a c c g a g a c t g a c a t g g t g g t a g g t g a t g t t g a t g t a c a c

1440 240 245 250 255 NSSCMGGMNRRPILTIITLE p53 NSSCMGGMNRRPILTIITLE t a a c a g t t c c t g c a t g g g c g g c a t g a a c c g g a g g c c c a t c c t c a c c a t c a t c a c a c t g g a a t t g t c a a g g a c g t a c c c g c c g t a c t t g g c c t c c g g g t a g g a g t g g t a g t a g t g t g a c c t

1500 260 265 270 275 DSSGNLLGRNSFEVRVCACH p53 DSSGNLLGRNSFEVRVCACH a g a c t c c a g t g g t a a t c t a c t g g g a c g g a a c a g c t t t g a g g t g c g t g t t t g t g c c t g t c a t c t g a g g t c a c c a t t a g a t g a c c c t g c c t t g t c g a a a c t c c a c g c a c a a a c a c g g a c a g t

BsaI 1560 280 285 290 295 GRDRRTEEENLRKKGEPHHE p53 GRDRRTEEENLRKKGEPHHE t g g g a g a g a c c g g c g c a c a g a g g a a g a g a a t c t c c g c a a g a a a g g g g a g c c t c a c c a c g a a c c c t c t c t g g c c g c g t g t c t c c t t c t c t t a g a g g c g t t c t t t c c c c t c g g a g t g g t g c t

PasI 1620 300 305 310 315 LPPGSTKRALPNNTSSSPQP p53 LPPGSTKRALPNNTSSSPQP g c t g c c c c c a g g g a g c a c t a a g c g a g c a c t g c c c a a c a a c a c c a g c t c c t c t c c c c a g c c c g a c g g g g g t c c c t c g t g a t t c g c t c g t g a c g g g t t g t t g t g g t c g a g g a g a g g g g t c g g

SspI 1680 320 325 330 335 KKKPLDGEYFTLQIRGRERF p53 KKKPLDGEYFTLQIRGRERF a a a g a a g a a a c c a c t g g a t g g a g a a t a t t t c a c c c t t c a g a t c c g t g g g c g t g a g c g c t t t t t c t t c t t t g g t g a c c t a c c t c t t a t a a a g t g g g a a g t c t a g g c a c c c g c a c t c g c g a a

StuI 1740 340 345 350 355 EMFRELNEALELKDAQAGKE p53 EMFRELNEALELKDAQAGKE c g a g a t g t t c c g a g a g c t g a a t g a g g c c t t g g a a c t c a a g g a t g c c c a g g c t g g g a a g g a g c t c t a c a a g g c t c t c g a c t t a c t c c g g a a c c t t g a g t t c c t a c g g g t c c g a c c c t t c c t

AccI 1800 360 365 370 375 PGGSRAHSSHLKSKKGQSTS p53 PGGSRAHSSHLKSKKGQSTS g c c a g g g g g g a g c a g g g c t c a c t c c a g c c a c c t g a a g t c c a a a a a g g g t c a g t c t a c c t c c g g t c c c c c c t c g t c c c g a g t g a g g t c g g t g g a c t t c a g g t t t t t c c c a g t c a g a t g g a g

1860 380 385 390 RHKKLMFKTEGPDSD* p53 attL2 RHKKLMFKTEGPDSD* c c g c c a t a a a a a a c t c a t g t t c a a g a c a g a a g g g c c t g a c t c a g a c t g a t t g c c a a c t t t g g c g g t a t t t t t t g a g t a c a a g t t c t g t c t t c c c g g a c t g a g t c t g a c t a a c g g t t g a a a

1920 attL2 c t t g t a c a a a g t t g g c a t t a t a a g a a a g c a t t g c t t a t c a a t t t g t t g c a a c g a a c a g g t g a a c a t g t t t c a a c c g t a a t a t t c t t t c g t a a c g a a t a g t t a a a c a a c g t t g c t t g t c c a

EcoRV 1980 attL2 T7 promoter G G G A T A T C A C T C A G T7 c a c t a t c a g t c a a a a t a a a a t c a t t a t t t g c c a t c c a g c t g a t a t c c c c t a t a g t g a g t c g t g a t a g t c a g t t t t a t t t t a g t a a t a a a c g g t a g g t c g a c t a t a g g g g a t a t c a c t c a g

2040 T7 promoter M13 rev C A T A A T T7 C A G T A T C G A C A A A G G A C M13 Reverse g t a t t a c a t g g t c a t a g c t g t t t c c t g g c a g c t c t g g c c c g t g t c t c a a a a t c t c t g a t g c a t a a t g t a c c a g t a t c g a c a a a g g a c c g t c g a g a c c g g g c a c a g a g t t t t a g a g a c t a c

XbaI 2100 t t a c a t t g c a c a a g a t a a a a a t a t a t c a t c a t g c c t c c t c t a g a c c a g c c a g g a c a g a a a a a t g t a a c g t g t t c t a t t t t t a t a t a g t a g t a c g g a g g a g a t c t g g t c g g t c c t g t c t t t

2160 t g c c t c g a c t t c g c t g c t a c c c a a g g t t g c c g g g t g a c g c a c a c c g t g g a a a c g g a t g a a a c g g a g c t g a a g c g a c g a t g g g t t c c a a c g g c c c a c t g c g t g t g g c a c c t t t g c c t a c t t

2220 M g g c a c g a a c c c a g t g g a c a t a a g c c t g t t c g g t t c g t a a g c t g t a a t g c a a g t a g c g t a t c c g t g c t t g g g t c a c c t g t a t t c g g a c a a g c c a a g c a t t c g a c a t t a c g t t c a t c g c a t a

2280 RSRNWSRTLTERSGGNGAVA g c g c t c a c g c a a c t g g t c c a g a a c c t t g a c c g a a c g c a g c g g t g g t a a c g g c g c a g t g g c c g c g a g t g c g t t g a c c a g g t c t t g g a a c t g g c t t g c g t c g c c a c c a t t g c c g c g t c a c c g

2340 VFMACYDCFFGVQSMPRASK g g t t t t c a t g g c t t g t t a t g a c t g t t t t t t t g g g g t a c a g t c t a t g c c t c g g g c a t c c a a c c a a a a g t a c c g a a c a a t a c t g a c a a a a a a a c c c c a t g t c a g a t a c g g a g c c c g t a g g t t

2400 QQARYAVGRCLMLWSSNDVT g c a g c a a g c g c g t t a c g c c g t g g g t c g a t g t t t g a t g t t a t g g a g c a g c a a c g a t g t t a c c g t c g t t c g c g c a a t g c g g c a c c c a g c t a c a a a c t a c a a t a c c t c g t c g t t g c t a c a a t g

2460 1 5 MREAVIAE SmR QQGSRPKTKLNIMREAVIAE g c a g c a g g g c a g t c g c c c t a a a a c a a a g t t a a a c a t t a t g a g g g a a g c g g t g a t c g c c g a c g t c g t c c c g t c a g c g g g a t t t t g t t t c a a t t t g t a a t a c t c c c t t c g c c a c t a g c g g c t

2520 10 15 20 25 VSTQLSEVVGVIERHLEPTL SmR VSTQLSEVVGVIERHLEPTL a g t a t c g a c t c a a c t a t c a g a g g t a g t t g g c g t c a t c g a g c g c c a t c t c g a a c c g a c g t t t c a t a g c t g a g t t g a t a g t c t c c a t c a a c c g c a g t a g c t c g c g g t a g a g c t t g g c t g c a a

2580 30 35 40 45 LAVHLYGSAVDGGLKPHSDI SmR LAVHLYGSAVDGGLKPHSDI g c t g g c c g t a c a t t t g t a c g g c t c c g c a g t g g a t g g c g g c c t g a a g c c a c a c a g t g a t a t c g a c c g g c a t g t a a a c a t g c c g a g g c g t c a c c t a c c g c c g g a c t t c g g t g t g t c a c t a t a

BclI*BstEII 2640 50 55 60 65 DLLVTVTVRLDETTRRALIN SmR DLLVTVTVRLDETTRRALIN t g a t t t g c t g g t t a c g g t g a c c g t a a g g c t t g a t g a a a c a a c g c g g c g a g c t t t g a t c a a a c t a a a c g a c c a a t g c c a c t g g c a t t c c g a a c t a c t t t g t t g c g c c g c t c g a a a c t a g t t

2700 70 75 80 85 DLLETSASPGESEILRAVEV SmR DLLETSASPGESEILRAVEV c g a c c t t t t g g a a a c t t c g g c t t c c c c t g g a g a g a g c g a g a t t c t c c g c g c t g t a g a a g t g c t g g a a a a c c t t t g a a g c c g a a g g g g a c c t c t c t c g c t c t a a g a g g c g c g a c a t c t t c a

BlpIMslI 2760 90 95 100 105 TIVVHDDIIPWRYPAKRELQ SmR TIVVHDDIIPWRYPAKRELQ c a c c a t t g t t g t g c a c g a c g a c a t c a t t c c g t g g c g t t a t c c a g c t a a g c g c g a a c t g c a g t g g t a a c a a c a c g t g c t g c t g t a g t a a g g c a c c g c a a t a g g t c g a t t c g c g c t t g a c g t

PvuI 2820 110 115 120 125 FGEWQRNDILAGIFEPATID SmR FGEWQRNDILAGIFEPATID a t t t g g a g a a t g g c a g c g c a a t g a c a t t c t t g c a g g t a t c t t c g a g c c a g c c a c g a t c g a t a a a c c t c t t a c c g t c g c g t t a c t g t a a g a a c g t c c a t a g a a g c t c g g t c g g t g c t a g c t

2880 130 135 140 145 IDLAILLTKAREHSVALVGP SmR IDLAILLTKAREHSVALVGP c a t t g a t c t g g c t a t c t t g c t g a c a a a a g c a a g a g a a c a t a g c g t t g c c t t g g t a g g t c c g t a a c t a g a c c g a t a g a a c g a c t g t t t t c g t t c t c t t g t a t c g c a a c g g a a c c a t c c a g g

2940 150 155 160 165 AAEELFDPVPEQDLFEALNE SmR AAEELFDPVPEQDLFEALNE a g c g g c g g a g g a a c t c t t t g a t c c g g t t c c t g a a c a g g a t c t a t t t g a g g c g c t a a a t g a t c g c c g c c t c c t t g a g a a a c t a g g c c a a g g a c t t g t c c t a g a t a a a c t c c g c g a t t t a c t

3000 170 175 180 185 TLTLWNSPPDWAGDERNVVL SmR TLTLWNSPPDWAGDERNVVL a a c c t t a a c g c t a t g g a a c t c g c c g c c c g a c t g g g c t g g c g a t g a g c g a a a t g t a g t g c t t t g g a a t t g c g a t a c c t t g a g c g g c g g g c t g a c c c g a c c g c t a c t c g c t t t a c a t c a c g a

3060 190 195 200 205 TLSRIWYSAVTGKIAPKDVA SmR TLSRIWYSAVTGKIAPKDVA t a c g t t g t c c c g c a t t t g g t a c a g c g c a g t a a c c g g c a a a a t c g c g c c g a a g g a t g t c g c a t g c a a c a g g g c g t a a a c c a t g t c g c g t c a t t g g c c g t t t t a g c g c g g c t t c c t a c a g c g

NgoMIVNaeIBglI 3120 210 215 220 225 ADWAMERLPAQYQPVILEAR SmR ADWAMERLPAQYQPVILEAR t g c c g a c t g g g c a a t g g a g c g c c t g c c g g c c c a g t a t c a g c c c g t c a t a c t t g a a g c t a g a c g g c t g a c c c g t t a c c t c g c g g a c g g c c g g g t c a t a g t c g g g c a g t a t g a a c t t c g a t c

BssHII 3180 230 235 240 245 QAYLGQEEDRLASRADQLEE SmR QAYLGQEEDRLASRADQLEE a c a g g c t t a t c t t g g a c a a g a a g a a g a t c g c t t g g c c t c g c g c g c a g a t c a g t t g g a a g a t g t c c g a a t a g a a c c t g t t c t t c t t c t a g c g a a c c g g a g c g c g c g t c t a g t c a a c c t t c t

PaeR7IPspXIXhoI 3240 250 255 260 FVHYVKGEITKVVGK* SmR FVHYVKGEITKVVGK* a t t t g t c c a c t a c g t g a a a g g c g a g a t c a c c a a g g t a g t c g g c a a a t a a c c c t c g a g c c a t a a a c a g g t g a t g c a c t t t c c g c t c t a g t g g t t c c a t c a g c c g t t t a t t g g g a g c t c g g t

3300 c c c a t g a c c a a a a t c c c t t a a c g t g a g t t a c g c g t c g t t c c a c t g a g c g t c a g a c c c c g t g g g t a c t g g t t t t a g g g a a t t g c a c t c a a t g c g c a g c a a g g t g a c t c g c a g t c t g g g g c a

3360 ori a g a a a a g a t c a a a g g a t c t t c t t g a g a t c c t t t t t t t c t g c g c g t a a t c t g c t g c t t g c a t c t t t t c t a g t t t c c t a g a a g a a c t c t a g g a a a a a a a g a c g c g c a t t a g a c g a c g a a c g t

3420 ori a a c a a a a a a a c c a c c g c t a c c a g c g g t g g t t t g t t t g c c g g a t c a a g a g c t a c c a a c t c t t t g t t t t t t t g g t g g c g a t g g t c g c c a c c a a a c a a a c g g c c t a g t t c t c g a t g g t t g a g a

3480 ori t t t t c c g a a g g t a a c t g g c t t c a g c a g a g c g c a g a t a c c a a a t a c t g t t c t t c t a g t g t a a a a a g g c t t c c a t t g a c c g a a g t c g t c t c g c g t c t a t g g t t t a t g a c a a g a a g a t c a c a t

3540 ori g c c g t a g t t a g g c c a c c a c t t c a a g a a c t c t g t a g c a c c g c c t a c a t a c c t c g c t c t g c t c g g c a t c a a t c c g g t g g t g a a g t t c t t g a g a c a t c g t g g c g g a t g t a t g g a g c g a g a c g a

3600 ori a a t c c t g t t a c c a g t g g c t g c t g c c a g t g g c g a t a a g t c g t g t c t t a c c g g g t t g g a c t c t t a g g a c a a t g g t c a c c g a c g a c g g t c a c c g c t a t t c a g c a c a g a a t g g c c c a a c c t g a g

3660 ori a a g a c g a t a g t t a c c g g a t a a g g c g c a g c g g t c g g g c t g a a c g g g g g g t t c g t g c a c a c a t t c t g c t a t c a a t g g c c t a t t c c g c g t c g c c a g c c c g a c t t g c c c c c c a a g c a c g t g t g t

3720 ori g c c c a g c t t g g a g c g a a c g a c c t a c a c c g a a c t g a g a t a c c t a c a g c g t g a g c t a t g a g a c g g g t c g a a c c t c g c t t g c t g g a t g t g g c t t g a c t c t a t g g a t g t c g c a c t c g a t a c t c t

BciVI 3780 ori a a g c g c c a c g c t t c c c g a a g g g a g a a a g g c g g a c a g g t a t c c g g t a a g c g g c a g g g t c g g t t c g c g g t g c g a a g g g c t t c c c t c t t t c c g c c t g t c c a t a g g c c a t t c g c c g t c c c a g c c

3840 ori G G G A A A C G C C T G G T A T C T T T pBR322ori-F a a c a g g a g a g c g c a c g a g g g a g c t t c c a g g g g g a a a c g c c t g g t a t c t t t a t a g t c c t g t t t g t c c t c t c g c g t g c t c c c t c g a a g g t c c c c c t t t g c g g a c c a t a g a a a t a t c a g g a c a

3900 ori c g g g t t t c g c c a c c t c t g a c t t g a g c g t c g a t t t t t g t g a t g c t c g t c a g g g g g g c g g a g g c c c a a a g c g g t g g a g a c t g a a c t c g c a g c t a a a a a c a c t a c g a g c a g t c c c c c c g c c t c

3960 ori c c t a t g g a a a a a c g c c a g c a a c g c g g c c t t t t t a c g g t t c c t g g c c t t t t g c t g g c c t t t g g a t a c c t t t t t g c g g t c g t t g c g c c g g a a a a a t g c c a a g g a c c g g a a a a c g a c c g g a a a

3972 t g c t c a c a t g t t a c g a g t g t a c a a

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.