Addgene pDrVSP-IRES2-EGFP Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: pDrVSP-IRES2-EGFP Sequencing Result


Map View

pBR322ori-F (6640 .. 6659) TK-pA-R (5731 .. 5750) BstBI (5559) Neo-F (5413 .. 5432) FspI (4979) PluTI (4880) SfoI (4878) NarI (4877) KasI (4876) Neo-R (4803 .. 4822) StuI (4698) SfiI (4652) SV40 promoter CsiI - SexAI * (4466) pBABE 3' (4380 .. 4400) F1ori-R (4118 .. 4137) F1ori-F (3906 .. 3927) AflII (3759) EBV-rev (3732 .. 3751) SV40pA-R (3678 .. 3697) HincII - HpaI (3640) MfeI (3627) XbaI * (3531) NotI (3521) AseI (59) SnaBI (392) CMV-F (571 .. 591) BspEI (791) BclI * (851) BstEII (1062) PstI (1405) Eco53kI (1685) SacI (1687) XcmI (1824) SacII (2197) AvaI - BsoBI - TspMI - XmaI (2201) SmaI (2203) AclI (2235) IRES reverse (2379 .. 2396) HindIII (2436) PmlI (2528) IRES-F (2606 .. 2625) BmgBI (2755) EGFP-N (2844 .. 2865) EXFP-R (3105 .. 3124) EGFP-C (3452 .. 3473) pDrVSP-IRES2-EGFP 6801 bp

Sequence View

AseI 5ʹ 3ʹ 60 c t t t c c t g c g t t a t c c c c t g a t t c t g t g g a t a a c c g t a t t a c c g c c a t g c a t t a g t t a t t g a a a g g a c g c a a t a g g g g a c t a a g a c a c c t a t t g g c a t a a t g g c g g t a c g t a a t c a a t a a

120 CMV enhancer a a t a g t a a t c a a t t a c g g g g t c a t t a g t t c a t a g c c c a t a t a t g g a g t t c c g c g t t a c a t t t a t c a t t a g t t a a t g c c c c a g t a a t c a a g t a t c g g g t a t a t a c c t c a a g g c g c a a t g t a

180 CMV enhancer a a c t t a c g g t a a a t g g c c c g c c t g g c t g a c c g c c c a a c g a c c c c c g c c c a t t g a c g t c a a t t g a a t g c c a t t t a c c g g g c g g a c c g a c t g g c g g g t t g c t g g g g g c g g g t a a c t g c a g t t

240 CMV enhancer t a a t g a c g t a t g t t c c c a t a g t a a c g c c a a t a g g g a c t t t c c a t t g a c g t c a a t g g g t g g a t t a c t g c a t a c a a g g g t a t c a t t g c g g t t a t c c c t g a a a g g t a a c t g c a g t t a c c c a c c

300 CMV enhancer a g t a t t t a c g g t a a a c t g c c c a c t t g g c a g t a c a t c a a g t g t a t c a t a t g c c a a g t a c g c t c a t a a a t g c c a t t t g a c g g g t g a a c c g t c a t g t a g t t c a c a t a g t a t a c g g t t c a t g c g

360 CMV enhancer c c c c t a t t g a c g t c a a t g a c g g t a a a t g g c c c g c c t g g c a t t a t g c c c a g t a c a t g a c c t g g g g a t a a c t g c a g t t a c t g c c a t t t a c c g g g c g g a c c g t a a t a c g g g t c a t g t a c t g g a

SnaBI 420 CMV enhancer CMV promoter t a t g g g a c t t t c c t a c t t g g c a g t a c a t c t a c g t a t t a g t c a t c g c t a t t a c c a t g g t g a a t a c c c t g a a a g g a t g a a c c g t c a t g t a g a t g c a t a a t c a g t a g c g a t a a t g g t a c c a c t

480 CMV promoter t g c g g t t t t g g c a g t a c a t c a a t g g g c g t g g a t a g c g g t t t g a c t c a c g g g g a t t t c c a a a c g c c a a a a c c g t c a t g t a g t t a c c c g c a c c t a t c g c c a a a c t g a g t g c c c c t a a a g g t t

540 CMV promoter g t c t c c a c c c c a t t g a c g t c a a t g g g a g t t t g t t t t g g c a c c a a a a t c a a c g g g a c t t t c c a g a g g t g g g g t a a c t g c a g t t a c c c t c a a a c a a a a c c g t g g t t t t a g t t g c c c t g a a a g

600 CMV promoter C G C A A A T G G G C G G T A G G C G T G CMV-F c a a a a t g t c g t a a c a a c t c c g c c c c a t t g a c g c a a a t g g g c g g t a g g c g t g t a c g g t g g g g t t t t a c a g c a t t g t t g a g g c g g g g t a a c t g c g t t t a c c c g c c a t c c g c a c a t g c c a c c c

660 CMV promoter M a g g t c t a t a t a a g c a g a g c t g g t t t a g t g a a c c g t c a g a t c c g c t a g a g c c g c c a c c a t g t c c a g a t a t a t t c g t c t c g a c c a a a t c a c t t g g c a g t c t a g g c g a t c t c g g c g g t g g t a c

720 TSVHFNPGLDSKEVNGNSVK a c g t c t g t g c a t t t t a a c c c t g g g t t a g a t t c c a a a g a a g t t a a t g g t a a t t c g g t g a a a t g c a g a c a c g t a a a a t t g g g a c c c a a t c t a a g g t t t c t t c a a t t a c c a t t a a g c c a c t t t

780 EEAEVQIGDGKEETKDPDTM g a a g a g g c a g a g g t g c a g a t c g g t g a t g g a a a g g a a g a a a c c a a a g a c c c a g a c a c a a t g c t t c t c c g t c t c c a c g t c t a g c c a c t a c c t t t c c t t c t t t g g t t t c t g g g t c t g t g t t a c

BspEI 840 YHQVRKKITPFVMSFGFRVF t a c c a t c a g g t c c g g a a a a a g a t a a c t c c t t t t g t g a t g t c c t t t g g t t t t c g t g t a t t t a t g g t a g t c c a g g c c t t t t t c t a t t g a g g a a a a c a c t a c a g g a a a c c a a a a g c a c a t a a a

BclI* 900 GLVLIILDIIMVIVDLSLSE g g c c t g g t a c t g a t c a t c t t g g a c a t c a t t a t g g t c a t t g t g g a c t t g t c t c t t t c t g a g c c g g a c c a t g a c t a g t a g a a c c t g t a g t a a t a c c a g t a a c a c c t g a a c a g a g a a a g a c t c

960 KSRDVGGALETVSLVISFFF a a g a g t c g t g a t g t t g g a g g t g c a c t g g a g a c t g t t t c t c t g g t c a t c t c t t t c t t t t t c t t c t c a g c a c t a c a a c c t c c a c g t g a c c t c t g a c a a a g a g a c c a g t a g a g a a a g a a a a a g

1020 LIDVLLRVYVEGFKVYFSSK c t c a t c g a t g t a t t g c t c c g t g t t t a t g t g g a g g g a t t c a a a g t g t a t t t c a g c t c a a a a g a g t a g c t a c a t a a c g a g g c a c a a a t a c a c c t c c c t a a g t t t c a c a t a a a g t c g a g t t t t

BstEII 1080 LNIVDACIVVITLVVTMIYA c t g a a t a t a g t a g a t g c t t g t a t t g t g g t c a t t a c t c t g g t g g t c a c c a t g a t c t a c g c a g a c t t a t a t c a t c t a c g a a c a t a a c a c c a g t a a t g a g a c c a c c a g t g g t a c t a g a t g c g t

1140 FSDFSGASLIPRVVTFLRSL t t c t c t g a t t t c t c a g g a g c c a g t c t g a t t c c c a g g g t g g t g a c a t t c c t g a g g t c t c t g a a g a g a c t a a a g a g t c c t c g g t c a g a c t a a g g g t c c c a c c a c t g t a a g g a c t c c a g a g a c

1200 RILILVRIFRLASQKRELEK a g g a t c c t a a t t c t g g t a c g c a t c t t c a g a c t g g c c t c a c a g a a g a g a g a g c t t g a a a a a t c c t a g g a t t a a g a c c a t g c g t a g a a g t c t g a c c g g a g t g t c t t c t c t c t c g a a c t t t t t

1260 VTRRMVSENKRRYQKDGFDL g t c a c t a g g a g a a t g g t t t c a g a g a a c a a a a g a c g g t a c c a a a a a g a t g g a t t t g a t c t g c a g t g a t c c t c t t a c c a a a g t c t c t t g t t t t c t g c c a t g g t t t t t c t a c c t a a a c t a g a c

1320 DLTYVTERVIAMSFPSSGKQ g a c c t c a c a t a c g t c a c a g a a a g a g t c a t c g c c a t g t c t t t t c c a t c t t c t g g g a a g c a g c t g g a g t g t a t g c a g t g t c t t t c t c a g t a g c g g t a c a g a a a a g g t a g a a g a c c c t t c g t c

1380 ALYRNPIREVVRFLDTKHMD g c g c t t t a t a g g a a c c c a a t c a g a g a g g t c g t t a g a t t c t t g g a c a c c a a a c a t a t g g a t c g c g a a a t a t c c t t g g g t t a g t c t c t c c a g c a a t c t a a g a a c c t g t g g t t t g t a t a c c t a

PstI 1440 HYKVFNLCSEKGYDPKFFHY c a t t a c a a g g t t t t t a a t c t c t g c a g t g a g a a a g g t t a t g a t c c a a a g t t c t t t c a c t a c g t a a t g t t c c a a a a a t t a g a g a c g t c a c t c t t t c c a a t a c t a g g t t t c a a g a a a g t g a t g

1500 RVERVMIDDHNVPSLDDMLR a g a g t a g a g c g t g t c a t g a t a g a t g a c c a t a a t g t c c c t t c a c t g g a t g a t a t g c t g c g c t c t c a t c t c g c a c a g t a c t a t c t a c t g g t a t t a c a g g g a a g t g a c c t a c t a t a c g a c g c g

1560 YTACVRDWMAADSRNVIAIH t a c a c c g c c t g t g t c a g a g a c t g g a t g g c a g c a g a c t c c a g g a a t g t t a t t g c c a t c c a c a t g t g g c g g a c a c a g t c t c t g a c c t a c c g t c g t c t g a g g t c c t t a c a a t a a c g g t a g g t g

1620 CKGGKGRTGTMVCTWLIDSD t g c a a a g g a g g a a a g g g t c g g a c c g g c a c t a t g g t t t g c a c a t g g c t c a t t g a c a g c g a t a c g t t t c c t c c t t t c c c a g c c t g g c c g t g a t a c c a a a c g t g t a c c g a g t a a c t g t c g c t a

1680 QFESAQESLDYFGERRTDKS c a g t t t g a g a g t g c a c a g g a g a g c c t g g a t t a c t t t g g a g a g a g a c g g a c t g a t a a a a g c g t c a a a c t c t c a c g t g t c c t c t c g g a c c t a a t g a a a c c t c t c t c t g c c t g a c t a t t t t c g

Eco53kISacI 1740 MSSKFQGVETPSQSRYVGYY a t g a g c t c c a a g t t t c a a g g t g t t g a a a c t c c g t c t c a g a g c a g g t a t g t t g g c t a t t a t t a c t c g a g g t t c a a a g t t c c a c a a c t t t g a g g c a g a g t c t c g t c c a t a c a a c c g a t a a t a

1800 EIMKNQYNRQLPPRKSLKIK g a g a t c a t g a a g a a t c a g t a c a a c c g t c a g c t t c c t c c t c g g a a g a g c c t g a a a a t c a a g c t c t a g t a c t t c t t a g t c a t g t t g g c a g t c g a a g g a g g a g c c t t c t c g g a c t t t t a g t t c

XcmI 1860 SIRIHSIAGVGKGNGSDLKL a g c a t c c g a a t c c a c t c c a t c g c a g g t g t g g g g a a g g g c a a c g g c a g t g a c c t g a a a c t g t c g t a g g c t t a g g t g a g g t a g c g t c c a c a c c c c t t c c c g t t g c c g t c a c t g g a c t t t g a c

1920 KIIVKHELVFQCVCAKQHNC a a g a t c a t a g t g a a g c a c g a g c t t g t t t t c c a g t g t g t t t g t g c c a a a c a g c a c a a c t g t t t c t a g t a t c a c t t c g t g c t c g a a c a a a a g g t c a c a c a a a c a c g g t t t g t c g t g t t g a c a

1980 TVFPDTGSNAVVISLQDGPI a c a g t g t t t c c t g a c a c t g g t a g c a a t g c g g t g g t c a t c a g t t t a c a g g a c g g g c c t a t a t g t c a c a a a g g a c t g t g a c c a t c g t t a c g c c a c c a g t a g t c a a a t g t c c t g c c c g g a t a t

2040 VTGDVKVMFESSAGLPKGYE g t g a c t g g a g a t g t g a a g g t c a t g t t t g a g t c c a g t g c t g g t t t g c c g a a g g g t t a t g a g c a c t g a c c t c t a c a c t t c c a g t a c a a a c t c a g g t c a c g a c c a a a c g g c t t c c c a a t a c t c

2100 DCPFYFWFNTSFVENYRLFL g a c t g t c c c t t c t a t t t c t g g t t c a a c a c c t c a t t t g t g g a g a a c t a c a g a c t t t t t c t g c t g a c a g g g a a g a t a a a g a c c a a g t t g t g g a g t a a a c a c c t c t t g a t g t c t g a a a a a g a c

2160 SREELDNPHKPKTWDIYKED t c g a g g g a a g a a c t g g a c a a t c c t c a c a a a c c c a a g a c c t g g g a c a t t t a c a a g g a a g a c a g c t c c c t t c t t g a c c t g t t a g g a g t g t t t g g g t t c t g g a c c c t g t a a a t g t t c c t t c t g

SacIITspMIBsoBIXmaIAvaISmaI 2220 IRES2 FGVTLSFTEP* t t t g g a g t g a c g c t g t c t t t c a c t g a g c c t t g a c c g c g g g c c c g g g a t c c g c c c c t c t c c a a a c c t c a c t g c g a c a g a a a g t g a c t c g g a a c t g g c g c c c g g g c c c t a g g c g g g g a g a g g

AclI 2280 IRES2 c t c c c c c c c c c c t a a c g t t a c t g g c c g a a g c c g c t t g g a a t a a g g c c g g t g t g c g t t t g t g a g g g g g g g g g g a t t g c a a t g a c c g g c t t c g g c g a a c c t t a t t c c g g c c a c a c g c a a a c a

2340 IRES2 c t a t a t g t t a t t t t c c a c c a t a t t g c c g t c t t t t g g c a a t g t g a g g g c c c g g a a a c c t g g g a t a t a c a a t a a a a g g t g g t a t a a c g g c a g a a a a c c g t t a c a c t c c c g g g c c t t t g g a c c

2400 IRES2 G A G A G C G G T T T C C T T A C G IRES reverse c c c t g t c t t c t t g a c g a g c a t t c c t a g g g g t c t t t c c c c t c t c g c c a a a g g a a t g c a a g g g g g a c a g a a g a a c t g c t c g t a a g g a t c c c c a g a a a g g g g a g a g c g g t t t c c t t a c g t t c c

HindIII 2460 IRES2 t c t g t t g a a t g t c g t g a a g g a a g c a g t t c c t c t g g a a g c t t c t t g a a g a c a a a c a a c g t c a g a c a a c t t a c a g c a c t t c c t t c g t c a a g g a g a c c t t c g a a g a a c t t c t g t t t g t t g c a g

2520 IRES2 t g t a g c g a c c c t t t g c a g g c a g c g g a a c c c c c c a c c t g g c g a c a g g t g c c t c t g c g g c c a a c a t c g c t g g g a a a c g t c c g t c g c c t t g g g g g g t g g a c c g c t g t c c a c g g a g a c g c c g g t

PmlI 2580 IRES2 a a a g c c a c g t g t a t a a g a t a c a c c t g c a a a g g c g g c a c a a c c c c a g t g c c a c g t t g t g a g t t t c g g t g c a c a t a t t c t a t g t g g a c g t t t c c g c c g t g t t g g g g t c a c g g t g c a a c a c t c

2640 IRES2 T G G C T C T C C T C A A G C G T A T T IRES-F t t g g a t a g t t g t g g a a a g a g t c a a a t g g c t c t c c t c a a g c g t a t t c a a c a a g g g g c t g a a a a c c t a t c a a c a c c t t t c t c a g t t t a c c g a g a g g a g t t c g c a t a a g t t g t t c c c c g a c t t

2700 IRES2 g g a t g c c c a g a a g g t a c c c c a t t g t a t g g g a t c t g a t c t g g g g c c t c g g t a c a c a t g c t t c c t a c g g g t c t t c c a t g g g g t a a c a t a c c c t a g a c t a g a c c c c g g a g c c a t g t g t a c g a a

BmgBI 2760 IRES2 t a c a t g t g t t t a g t c g a g g t t a a a a a a a c g t c t a g g c c c c c c g a a c c a c g g g g a c g t g g t a t g t a c a c a a a t c a g c t c c a a t t t t t t t g c a g a t c c g g g g g g c t t g g t g c c c c t g c a c c a

2820 ATG 1 M 1a V 5 SKGEE IRES2 EGFP 1 M 1a V 5 SKGEE EGFP MATTMVSKGEE t t t c c t t t g a a a a a c a c g a t g a t a a t a t g g c c a c a a c c a t g g t g a g c a a g g g c g a g g a g c a a a g g a a a c t t t t t g t g c t a c t a t t a t a c c g g t g t t g g t a c c a c t c g t t c c c g c t c c t c g

2880 10 15 20 25 LFTGVVPILVELDGDVNGHK EGFP 10 15 20 25 LFTGVVPILVELDGDVNGHK EGFP G A C C A G C T C G A C C T G C C G C T G C EGFP-N LFTGVVPILVELDGDVNGHK t g t t c a c c g g g g t g g t g c c c a t c c t g g t c g a g c t g g a c g g c g a c g t a a a c g g c c a c a a g t a c a a g t g g c c c c a c c a c g g g t a g g a c c a g c t c g a c c t g c c g c t g c a t t t g c c g g t g t t c a

2940 30 35 40 45 FSVSGEGEGDATYGKLTLKF EGFP 30 35 40 45 FSVSGEGEGDATYGKLTLKF EGFP FSVSGEGEGDATYGKLTLKF t c a g c g t g t c c g g c g a g g g c g a g g g c g a t g c c a c c t a c g g c a a g c t g a c c c t g a a g t t c a a g t c g c a c a g g c c g c t c c c g c t c c c g c t a c g g t g g a t g c c g t t c g a c t g g g a c t t c a a g t

3000 50 55 60 65 ICTTGKLPVPWPTLVTTLTY EGFP 50 55 60 65 ICTTGKLPVPWPTLVTTLTY EGFP ICTTGKLPVPWPTLVTTLTY t c t g c a c c a c c g g c a a g c t g c c c g t g c c c t g g c c c a c c c t c g t g a c c a c c c t g a c c t a c g a g a c g t g g t g g c c g t t c g a c g g g c a c g g g a c c g g g t g g g a g c a c t g g t g g g a c t g g a t g c

3060 70 75 80 85 GVQCFSRYPDHMKQHDFFKS EGFP 70 75 80 85 GVQCFSRYPDHMKQHDFFKS EGFP GVQCFSRYPDHMKQHDFFKS g c g t g c a g t g c t t c a g c c g c t a c c c c g a c c a c a t g a a g c a g c a c g a c t t c t t c a a g t c c g c g c a c g t c a c g a a g t c g g c g a t g g g g c t g g t g t a c t t c g t c g t g c t g a a g a a g t t c a g g c

3120 90 95 100 105 AMPEGYVQERTIFFKDDGNY EGFP 90 95 100 105 AMPEGYVQERTIFFKDDGNY EGFP C T G C T G C C G T T G A T G T EXFP-R AMPEGYVQERTIFFKDDGNY c c a t g c c c g a a g g c t a c g t c c a g g a g c g c a c c a t c t t c t t c a a g g a c g a c g g c a a c t a c a g g t a c g g g c t t c c g a t g c a g g t c c t c g c g t g g t a g a a g a a g t t c c t g c t g c c g t t g a t g t

3180 110 115 120 125 KTRAEVKFEGDTLVNRIELK EGFP 110 115 120 125 KTRAEVKFEGDTLVNRIELK EGFP T C T G EXFP-R KTRAEVKFEGDTLVNRIELK a g a c c c g c g c c g a g g t g a a g t t c g a g g g c g a c a c c c t g g t g a a c c g c a t c g a g c t g a a g g t c t g g g c g c g g c t c c a c t t c a a g c t c c c g c t g t g g g a c c a c t t g g c g t a g c t c g a c t t c c

3240 130 135 140 145 GIDFKEDGNILGHKLEYNYN EGFP 130 135 140 145 GIDFKEDGNILGHKLEYNYN EGFP GIDFKEDGNILGHKLEYNYN g c a t c g a c t t c a a g g a g g a c g g c a a c a t c c t g g g g c a c a a g c t g g a g t a c a a c t a c a a c a c g t a g c t g a a g t t c c t c c t g c c g t t g t a g g a c c c c g t g t t c g a c c t c a t g t t g a t g t t g t

3300 150 155 160 165 SHNVYIMADKQKNGIKVNFK EGFP 150 155 160 165 SHNVYIMADKQKNGIKVNFK EGFP SHNVYIMADKQKNGIKVNFK g c c a c a a c g t c t a t a t c a t g g c c g a c a a g c a g a a g a a c g g c a t c a a g g t g a a c t t c a a g a c g g t g t t g c a g a t a t a g t a c c g g c t g t t c g t c t t c t t g c c g t a g t t c c a c t t g a a g t t c t

3360 170 175 180 185 IRHNIEDGSVQLADHYQQNT EGFP 170 175 180 185 IRHNIEDGSVQLADHYQQNT EGFP IRHNIEDGSVQLADHYQQNT t c c g c c a c a a c a t c g a g g a c g g c a g c g t g c a g c t c g c c g a c c a c t a c c a g c a g a a c a c c c a g g c g g t g t t g t a g c t c c t g c c g t c g c a c g t c g a g c g g c t g g t g a t g g t c g t c t t g t g g g

3420 190 195 200 205 PIGDGPVLLPDNHYLSTQSA EGFP 190 195 200 205 PIGDGPVLLPDNHYLSTQSA EGFP PIGDGPVLLPDNHYLSTQSA c c a t c g g c g a c g g c c c c g t g c t g c t g c c c g a c a a c c a c t a c c t g a g c a c c c a g t c c g c c c g g t a g c c g c t g c c g g g g c a c g a c g a c g g g c t g t t g g t g a t g g a c t c g t g g g t c a g g c g g g

3480 210 215 220 225 LSKDPNEKRDHMVLLEFVTA EGFP 210 215 220 225 LSKDPNEKRDHMVLLEFVTA EGFP C A T G G T C C T G C T G G A G T T C G T G EGFP-C LSKDPNEKRDHMVLLEFVTA t g a g c a a a g a c c c c a a c g a g a a g c g c g a t c a c a t g g t c c t g c t g g a g t t c g t g a c c g c c g a c t c g t t t c t g g g g t t g c t c t t c g c g c t a g t g t a c c a g g a c g a c c t c a a g c a c t g g c g g c

XbaI*NotI 3540 230 235 AGITLGMDELYK* EGFP 230 235 AGITLGMDELYK * EGFP AGITLGMDELYK* c c g g g a t c a c t c t c g g c a t g g a c g a g c t g t a c a a g t a a a g c g g c c g c g a c t c t a g a t c a t g g c c c t a g t g a g a g c c g t a c c t g c t c g a c a t g t t c a t t t c g c c g g c g c t g a g a t c t a g t a

3600 a a t c a g c c a t a c c a c a t t t g t a g a g g t t t t a c t t g c t t t a a a a a a c c t c c c a c a c c t c c c t t a g t c g g t a t g g t g t a a a c a t c t c c a a a a t g a a c g a a a t t t t t t g g a g g g t g t g g a g g g

HincIIHpaIMfeI 3660 SV40 poly(A) signal c c t g a a c c t g a a a c a t a a a a t g a a t g c a a t t g t t g t t g t t a a c t t g t t t a t t g c a g c t t a g g a c t t g g a c t t t g t a t t t t a c t t a c g t t a a c a a c a a c a a t t g a a c a a a t a a c g t c g a a t

3720 SV40 poly(A) signal C G T T A T C G T A G T G T T T A A A G SV40pA-R t a a t g g t t a c a a a t a a a g c a a t a g c a t c a c a a a t t t c a c a a a t a a a g c a t t t t t t t c a c t a t t a c c a a t g t t t a t t t c g t t a t c g t a g t g t t t a a a g t g t t t a t t t c g t a a a a a a a g t g a

AflII 3780 SV40 poly(A) signal f1 ori G T G G T T T G T C C A A A C T C A T C EBV-rev g c a t t c t a g t t g t g g t t t g t c c a a a c t c a t c a a t g t a t c t t a a g g c g t a a a t t g t a a g c g c g t a a g a t c a a c a c c a a a c a g g t t t g a g t a g t t a c a t a g a a t t c c g c a t t t a a c a t t c g c

3840 f1 ori t t a a t a t t t t g t t a a a a t t c g c g t t a a a t t t t t g t t a a a t c a g c t c a t t t t t t a a c c a a t a a t t a t a a a a c a a t t t t a a g c g c a a t t t a a a a a c a a t t t a g t c g a g t a a a a a a t t g g t t a

3900 f1 ori a g g c c g a a a t c g g c a a a a t c c c t t a t a a a t c a a a a g a a t a g a c c g a g a t a g g g t t g a g t g t c c g g c t t t a g c c g t t t t a g g g a a t a t t t a g t t t t c t t a t c t g g c t c t a t c c c a a c t c a c

3960 f1 ori G G T C A A A C C T T G T T C T C A G G T G F1ori-F t t g t t c c a g t t t g g a a c a a g a g t c c a c t a t t a a a g a a c g t g g a c t c c a a c g t c a a a g g g c a a c a a g g t c a a a c c t t g t t c t c a g g t g a t a a t t t c t t g c a c c t g a g g t t g c a g t t t c c c g

4020 f1 ori g a a a a a c c g t c t a t c a g g g c g a t g g c c c a c t a c g t g a a c c a t c a c c c t a a t c a a g t t t t t c t t t t t g g c a g a t a g t c c c g c t a c c g g g t g a t g c a c t t g g t a g t g g g a t t a g t t c a a a a a

4080 f1 ori t g g g g t c g a g g t g c c g t a a a g c a c t a a a t c g g a a c c c t a a a g g g a g c c c c c g a t t t a g a g a c c c c a g c t c c a c g g c a t t t c g t g a t t t a g c c t t g g g a t t t c c c t c g g g g g c t a a a t c t c

4140 f1 ori A G G G A A G A A A G C G A A A G G A G F1ori-R c t t g a c g g g g a a a g c c g g c g a a c g t g g c g a g a a a g g a a g g g a a g a a a g c g a a a g g a g c g g g a a c t g c c c c t t t c g g c c g c t t g c a c c g c t c t t t c c t t c c c t t c t t t c g c t t t c c t c g c c

4200 f1 ori g c g c t a g g g c g c t g g c a a g t g t a g c g g t c a c g c t g c g c g t a a c c a c c a c a c c c g c c g c g c c g c g a t c c c g c g a c c g t t c a c a t c g c c a g t g c g a c g c g c a t t g g t g g t g t g g g c g g c g c g

4260 f1 ori AmpR promoter t t a a t g c g c c g c t a c a g g g c g c g t c a g g t g g c a c t t t t c g g g g a a a t g t g c g c g g a a c c c a a t t a c g c g g c g a t g t c c c g c g c a g t c c a c c g t g a a a a g c c c c t t t a c a c g c g c c t t g g g

4320 AmpR promoter c t a t t t g t t t a t t t t t c t a a a t a c a t t c a a a t a t g t a t c c g c t c a t g a g a c a a t a a c c c t g a t a a a c a a a t a a a a a g a t t t a t g t a a g t t t a t a c a t a g g c g a g t a c t c t g t t a t t g g g a

4380 AmpR promoter SV40 promoter SV40 enhancer C pBABE 3' g a t a a a t g c t t c a a t a a t a t t g a a a a a g g a a g a g t c c t g a g g c g g a a a g a a c c a g c t g t g c t a t t t a c g a a g t t a t t a t a a c t t t t t c c t t c t c a g g a c t c c g c c t t t c t t g g t c g a c a c

4440 SV40 promoter SV40 enhancer C T T A C A C A C A G T C A A T C C C A pBABE 3' g a a t g t g t g t c a g t t a g g g t g t g g a a a g t c c c c a g g c t c c c c a g c a g g c a g a a g t a t g c a c t t a c a c a c a g t c a a t c c c a c a c c t t t c a g g g g t c c g a g g g g t c g t c c g t c t t c a t a c g t

SexAI*CsiI 4500 SV40 promoter SV40 enhancer a a g c a t g c a t c t c a a t t a g t c a g c a a c c a g g t g t g g a a a g t c c c c a g g c t c c c c a g c a g g t t c g t a c g t a g a g t t a a t c a g t c g t t g g t c c a c a c c t t t c a g g g g t c c g a g g g g t c g t c c

4560 SV40 promoter SV40 enhancer c a g a a g t a t g c a a a g c a t g c a t c t c a a t t a g t c a g c a a c c a t a g t c c c g c c c c t a a c t c c g t c t t c a t a c g t t t c g t a c g t a g a g t t a a t c a g t c g t t g g t a t c a g g g c g g g g a t t g a g g

4620 SV40 promoter SV40 ori g c c c a t c c c g c c c c t a a c t c c g c c c a g t t c c g c c c a t t c t c c g c c c c a t g g c t g a c t a a t c g g g t a g g g c g g g g a t t g a g g c g g g t c a a g g c g g g t a a g a g g c g g g g t a c c g a c t g a t t a

SfiI 4680 SV40 promoter SV40 ori t t t t t t t a t t t a t g c a g a g g c c g a g g c c g c c t c g g c c t c t g a g c t a t t c c a g a a g t a g t g a a a a a a a t a a a t a c g t c t c c g g c t c c g g c g g a g c c g g a g a c t c g a t a a g g t c t t c a t c a c

StuI 4740 SV40 promoter SV40 ori a g g a g g c t t t t t t g g a g g c c t a g g c t t t t g c a a a g a t c g a t c a a g a g a c a g g a t g a g g a t t c c t c c g a a a a a a c c t c c g g a t c c g a a a a c g t t t c t a g c t a g t t c t c t g t c c t a c t c c t a

4800 1 5 10 15 MIEQDGLHAGSPAAWVE NeoR/KanR 1 5 10 15 MIEQDGLHAGSPAAWVE NeoR/KanR MIEQDGLHAGSPAAWVE c g t t t c g c a t g a t t g a a c a a g a t g g a t t g c a c g c a g g t t c t c c g g c c g c t t g g g t g g a g a g c a a a g c g t a c t a a c t t g t t c t a c c t a a c g t g c g t c c a a g a g g c c g g c g a a c c c a c c t c t

4860 20 25 30 35 RLFGYDWAQQTIGCSDAAVF NeoR/KanR 20 25 30 35 RLFGYDWAQQTIGCSDAAVF NeoR/KanR G A T A A G C C G A T A C T G A C C C G Neo-R RLFGYDWAQQTIGCSDAAVF g g c t a t t c g g c t a t g a c t g g g c a c a a c a g a c a a t c g g c t g c t c t g a t g c c g c c g t g t t c c c c g a t a a g c c g a t a c t g a c c c g t g t t g t c t g t t a g c c g a c g a g a c t a c g g c g g c a c a a g g

KasINarISfoIPluTI 4920 40 45 50 55 RLSAQGRPVLFVKTDLSGAL NeoR/KanR 40 45 50 55 RLSAQGRPVLFVKTDLSGAL NeoR/KanR RLSAQGRPVLFVKTDLSGAL g g c t g t c a g c g c a g g g g c g c c c g g t t c t t t t t g t c a a g a c c g a c c t g t c c g g t g c c c t g a c c g a c a g t c g c g t c c c c g c g g g c c a a g a a a a a c a g t t c t g g c t g g a c a g g c c a c g g g a c t

FspI 4980 60 65 70 75 NELQDEAARLSWLATTGVPC NeoR/KanR 60 65 70 75 NELQDEAARLSWLATTGVPC NeoR/KanR NELQDEAARLSWLATTGVPC a t g a a c t g c a a g a c g a g g c a g c g c g g c t a t c g t g g c t g g c c a c g a c g g g c g t t c c t t g c g t a c t t g a c g t t c t g c t c c g t c g c g c c g a t a g c a c c g a c c g g t g c t g c c c g c a a g g a a c g c

5040 80 85 90 95 AAVLDVVTEAGRDWLLLGEV NeoR/KanR 80 85 90 95 AAVLDVVTEAGRDWLLLGEV NeoR/KanR AAVLDVVTEAGRDWLLLGEV c a g c t g t g c t c g a c g t t g t c a c t g a a g c g g g a a g g g a c t g g c t g c t a t t g g g c g a a g t g c g t c g a c a c g a g c t g c a a c a g t g a c t t c g c c c t t c c c t g a c c g a c g a t a a c c c g c t t c a c g

5100 100 105 110 115 PGQDLLSSHLAPAEKVSIMA NeoR/KanR 100 105 110 115 PGQDLLSSHLAPAEKVSIMA NeoR/KanR PGQDLLSSHLAPAEKVSIMA *RVKSRGLFYGDHS c g g g g c a g g a t c t c c t g t c a t c t c a c c t t g c t c c t g c c g a g a a a g t a t c c a t c a t g g c t g g c c c c g t c c t a g a g g a c a g t a g a g t g g a a c g a g g a c g g c t c t t t c a t a g g t a g t a c c g a c

5160 120 125 130 135 DAMRRLHTLDPATCPFDHQA NeoR/KanR 120 125 130 135 DAMRRLHTLDPATCPFDHQA NeoR/KanR DAMRRLHTLDPATCPFDHQA ICHPPQMRKIRSGAWEVVLR a t g c a a t g c g g c g g c t g c a t a c g c t t g a t c c g g c t a c c t g c c c a t t c g a c c a c c a a g c g a t a c g t t a c g c c g c c g a c g t a t g c g a a c t a g g c c g a t g g a c g g g t a a g c t g g t g g t t c g c t

5220 140 145 150 155 KHRIERARTRMEAGLVDQDD NeoR/KanR 140 145 150 155 KHRIERARTRMEAGLVDQDD NeoR/KanR KHRIERARTRMEAGLVDQDD FMADLSCTSPHFGTKDILII a a c a t c g c a t c g a g c g a g c a c g t a c t c g g a t g g a a g c c g g t c t t g t c g a t c a g g a t g a t c t t g t a g c g t a g c t c g c t c g t g c a t g a g c c t a c c t t c g g c c a g a a c a g c t a g t c c t a c t a g

5280 160 165 170 175 LDEEHQGLAPAELFARLKAS NeoR/KanR 160 165 170 175 LDEEHQGLAPAELFARLKAS NeoR/KanR LDEEHQGLAPAELFARLKAS QVFLMLPERWGFQEGPELRA t g g a c g a a g a g c a t c a g g g g c t c g c g c c a g c c g a a c t g t t c g c c a g g c t c a a g g c g a g c a a c c t g c t t c t c g t a g t c c c c g a g c g c g g t c g g c t t g a c a a g c g g t c c g a g t t c c g c t c g t

5340 180 185 190 195 MPDGEDLVVTHGDACLPNIM NeoR/KanR 180 185 190 195 MPDGEDLVVTHGDACLPNIM NeoR/KanR MPDGEDLVVTHGDACLPNIM HGVALIEDHGMAIGAQRIDH t g c c c g a c g g c g a g g a t c t c g t c g t g a c c c a t g g c g a t g c c t g c t t g c c g a a t a t c a t g g a c g g g c t g c c g c t c c t a g a g c a g c a c t g g g t a c c g c t a c g g a c g a a c g g c t t a t a g t a c c

5400 200 205 210 215 VENGRFSGFIDCGRLGVADR NeoR/KanR 200 205 210 215 VENGRFSGFIDCGRLGVADR NeoR/KanR VENGRFSGFIDCGRLGVADR HFIAAKRSEDVTAPQTHRVA t g g a a a a t g g c c g c t t t t c t g g a t t c a t c g a c t g t g g c c g g c t g g g t g t g g c g g a c c g c t a c c t t t t a c c g g c g a a a a g a c c t a a g t a g c t g a c a c c g g c c g a c c c a c a c c g c c t g g c g a

5460 220 225 230 235 YQDIALATRDIAEELGGEWA NeoR/KanR 220 225 230 235 YQDIALATRDIAEELGGEWA NeoR/KanR C G T T G G C T A C C C G T G A T A T T Neo-F YQDIALATRDIAEELGGEWA ILVYRQSGTINSFLKAAFPS a t c a g g a c a t a g c g t t g g c t a c c c g t g a t a t t g c t g a a g a g c t t g g c g g c g a a t g g g c t g t a g t c c t g t a t c g c a a c c g a t g g g c a c t a t a a c g a c t t c t c g a a c c g c c g c t t a c c c g a c

5520 240 245 250 255 DRFLVLYGIAAPDSQRIAFY NeoR/KanR 240 245 250 255 DRFLVLYGIAAPDSQRIAFY NeoR/KanR DRFLVLYGIAAPDSQRIAFY VAEEHKVTDGSGIRLADGEI a c c g c t t c c t c g t g c t t t a c g g t a t c g c c g c t c c c g a t t c g c a g c g c a t c g c c t t c t a t c t g g c g a a g g a g c a c g a a a t g c c a t a g c g g c g a g g g c t a a g c g t c g c g t a g c g g a a g a t a g

BstBI 5580 260 265 RLLDEFF* NeoR/KanR 260 265 RLLDEFF* NeoR/KanR MTDQAT RLLDEFF* AKKVLEESRSEPTRFSRGLS g c c t t c t t g a c g a g t t c t t c t g a g c g g g a c t c t g g g g t t c g a a a t g a c c g a c c a a g c g a c c g g a a g a a c t g c t c a a g a a g a c t c g c c c t g a g a c c c c a a g c t t t a c t g g c t g g t t c g c t g

5640 PNLPSRDFDSTAAFYERLGF AWGAM g c c c a a c c t g c c a t c a c g a g a t t t c g a t t c c a c c g c c g c c t t c t a t g a a a g g t t g g g c t t c g g g t t g g a c g g t a g t g c t c t a a a g c t a a g g t g g c g g c g g a a g a t a c t t t c c a a c c c g a a

5700 GIVFRDAGWMILQRGDLMLE c g g a a t c g t t t t c c g g g a c g c c g g c t g g a t g a t c c t c c a g c g c g g g g a t c t c a t g c t g g a g c c t t a g c a a a a g g c c c t g c g g c c g a c c t a c t a g g a g g t c g c g c c c c t a g a g t a c g a c c t

5760 A C T T T G T G C C T T C C T C T G T T TK-pA-R FFAHPRGRLTETRKETIPEG g t t c t t c g c c c a c c c t a g g g g g a g g c t a a c t g a a a c a c g g a a g g a g a c a a t a c c g g a a g g c a a g a a g c g g g t g g g a t c c c c c t c c g a t t g a c t t t g t g c c t t c c t c t g t t a t g g c c t t c c

5820 HSV TK poly(A) signal TRAMTAIKRQNKTHGVGSFV a a c c c g c g c t a t g a c g g c a a t a a a a a g a c a g a a t a a a a c g c a c g g t g t t g g g t c g t t t g t t t g g g c g c g a t a c t g c c g t t a t t t t t c t g t c t t a t t t t g c g t g c c a c a a c c c a g c a a a c a

5880 HSV TK poly(A) signal HKRGVRSQGWHSVDTPPRPH t c a t a a a c g c g g g g t t c g g t c c c a g g g c t g g c a c t c t g t c g a t a c c c c a c c g a g a c c c c a a g t a t t t g c g c c c c a a g c c a g g g t c c c g a c c g t g a g a c a g c t a t g g g g t g g c t c t g g g g t

5940 WGQYARVSSFSPPHPPSSGE t t g g g g c c a a t a c g c c c g c g t t t c t t c c t t t t c c c c a c c c c a c c c c c c a a g t t c g g g t g a a a c c c c g g t t a t g c g g g c g c a a a g a a g g a a a a g g g g t g g g g t g g g g g g t t c a a g c c c a c t

6000 GPGLAANVGAAGPAIASGYS a g g c c c a g g g c t c g c a g c c a a c g t c g g g g c g g c a g g c c c t g c c a t a g c c t c a g g t t a c t c t c c g g g t c c c g a g c g t c g g t t g c a g c c c c g c c g t c c g g g a c g g t a t c g g a g t c c a a t g a g

6060 YIL* a t a t a t a c t t t a g a t t g a t t t a a a a c t t c a t t t t t a a t t t a a a a g g a t c t a g g t g a a g a t t a t a t a t g a a a t c t a a c t a a a t t t t g a a g t a a a a a t t a a a t t t t c c t a g a t c c a c t t c t a

6120 c c t t t t t g a t a a t c t c a t g a c c a a a a t c c c t t a a c g t g a g t t t t c g t t c c a c t g a g c g t c g g a a a a a c t a t t a g a g t a c t g g t t t t a g g g a a t t g c a c t c a a a a g c a a g g t g a c t c g c a g

6180 ori a g a c c c c g t a g a a a a g a t c a a a g g a t c t t c t t g a g a t c c t t t t t t t c t g c g c g t a a t c t g t c t g g g g c a t c t t t t c t a g t t t c c t a g a a g a a c t c t a g g a a a a a a a g a c g c g c a t t a g a c

6240 ori c t g c t t g c a a a c a a a a a a a c c a c c g c t a c c a g c g g t g g t t t g t t t g c c g g a t c a a g a g c t g a c g a a c g t t t g t t t t t t t g g t g g c g a t g g t c g c c a c c a a a c a a a c g g c c t a g t t c t c g a

6300 ori a c c a a c t c t t t t t c c g a a g g t a a c t g g c t t c a g c a g a g c g c a g a t a c c a a a t a c t g t t c t t g g t t g a g a a a a a g g c t t c c a t t g a c c g a a g t c g t c t c g c g t c t a t g g t t t a t g a c a a g a

6360 ori t c t a g t g t a g c c g t a g t t a g g c c a c c a c t t c a a g a a c t c t g t a g c a c c g c c t a c a t a c c t a g a t c a c a t c g g c a t c a a t c c g g t g g t g a a g t t c t t g a g a c a t c g t g g c g g a t g t a t g g a

6420 ori c g c t c t g c t a a t c c t g t t a c c a g t g g c t g c t g c c a g t g g c g a t a a g t c g t g t c t t a c c g g g c g a g a c g a t t a g g a c a a t g g t c a c c g a c g a c g g t c a c c g c t a t t c a g c a c a g a a t g g c c

6480 ori g t t g g a c t c a a g a c g a t a g t t a c c g g a t a a g g c g c a g c g g t c g g g c t g a a c g g g g g g t t c c a a c c t g a g t t c t g c t a t c a a t g g c c t a t t c c g c g t c g c c a g c c c g a c t t g c c c c c c a a g

6540 ori g t g c a c a c a g c c c a g c t t g g a g c g a a c g a c c t a c a c c g a a c t g a g a t a c c t a c a g c g t g a c a c g t g t g t c g g g t c g a a c c t c g c t t g c t g g a t g t g g c t t g a c t c t a t g g a t g t c g c a c t

6600 ori g c t a t g a g a a a g c g c c a c g c t t c c c g a a g g g a g a a a g g c g g a c a g g t a t c c g g t a a g c g g c g a t a c t c t t t c g c g g t g c g a a g g g c t t c c c t c t t t c c g c c t g t c c a t a g g c c a t t c g c c

6660 ori G G G A A A C G C C T G G T A T C T T T pBR322ori-F c a g g g t c g g a a c a g g a g a g c g c a c g a g g g a g c t t c c a g g g g g a a a c g c c t g g t a t c t t t a g t c c c a g c c t t g t c c t c t c g c g t g c t c c c t c g a a g g t c c c c c t t t g c g g a c c a t a g a a a t

6720 ori t a g t c c t g t c g g g t t t c g c c a c c t c t g a c t t g a g c g t c g a t t t t t g t g a t g c t c g t c a g g a t c a g g a c a g c c c a a a g c g g t g g a g a c t g a a c t c g c a g c t a a a a a c a c t a c g a g c a g t c c

6780 ori g g g g c g g a g c c t a t g g a a a a a c g c c a g c a a c g c g g c c t t t t t a c g g t t c c t g g c c t t t t g c c c c g c c t c g g a t a c c t t t t t g c g g t c g t t g c g c c g g a a a a a t g c c a a g g a c c g g a a a a c

3ʹ 5ʹ 6801 c t g g c c t t t t g c t c a c a t g t t g a c c g g a a a a c g a g t g t a c a a

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.