Addgene pUC-TALO8 Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pUC-TALO8 Sequencing Result

Map View

800 600 400 200 End (842) BsaHI (687) HindIII (656) BmrI (638) BfuAI - PaqCI - BspMI (576) BsgI (556) MslI (445) BstXI (441) EcoRV (428) Bsu36I (385) MfeI (283) BaeGI - Bme1580I (268) PspFI (252) BseYI (248) BstAPI (222) AseI (154) BstZ17I (110) PmlI (103) BsiEI (49) EcoRI (41) SacI (39) Eco53kI (37) TaqII (35) KpnI (33) BanI - Acc65I (29) SmaI (27) AvaI - XmaI - BsoBI - TspMI (25) MflI * - BstYI - BamHI (20) HincII (10) SalI (8) PstI - SbfI (6) SfcI (2) Start (0) TRP1 promoter TRP1 pUC-TALO8 842 bp

Sequence View

BsiEIBamHIBstYIMflI*TspMIBsoBIXmaIAvaISmaIAcc65IBanIKpnITaqIIEco53kISacIEcoRIStart (0) SfcISbfIPstISalIHincII 5ʹ 3ʹ 60 TRP1 promoter c c t g c a g g t c g a c t c t a g a g g a t c c c c g g g t a c c g a g c t c g a a t t c g g t c g a a a a a a g a a g g a c g t c c a g c t g a g a t c t c c t a g g g g c c c a t g g c t c g a g c t t a a g c c a g c t t t t t t c t t

BstZ17IPmlI 120 TRP1 promoter a a g g a g a g g g c c a a g a g g g a g g g c a t t g g t g a c t a t t g a g c a c g t g a g t a t a c g t g a t t a t t c c t c t c c c g g t t c t c c c t c c c g t a a c c a c t g a t a a c t c g t g c a c t c a t a t g c a c t a a t

AseI 180 1 5 10 MSVINFTGSSGP TRP1 promoter TRP1 MSVINFTGSSGP a g c a c a c a a a g g c a g c t t g g a g t a t g t c t g t t a t t a a t t t c a c a g g t a g t t c t g g t c c a t t c g t g t g t t t c c g t c g a a c c t c a t a c a g a c a a t a a t t a a a g t g t c c a t c a a g a c c a g g t a

BstAPI 240 15 20 25 30 LVKVCGLQSTEAAECALDSD TRP1 LVKVCGLQSTEAAECALDSD t g g t g a a a g t t t g c g g c t t g c a g a g c a c a g a g g c c g c a g a a t g t g c t c t a g a t t c c g a t g a c c a c t t t c a a a c g c c g a a c g t c t c g t g t c t c c g g c g t c t t a c a c g a g a t c t a a g g c t a c

MfeIBme1580IBaeGIBseYIPspFI 300 35 40 45 50 ADLLGIICVPNRKRTIDPVI TRP1 ADLLGIICVPNRKRTIDPVI c t g a c t t g c t g g g t a t t a t a t g t g t g c c c a a t a g a a a g a g a a c a a t t g a c c c g g t t a t t g g a c t g a a c g a c c c a t a a t a t a c a c a c g g g t t a t c t t t c t c t t g t t a a c t g g g c c a a t a a c

360 55 60 65 70 ARKISSLVKAYKNSSGTPKY TRP1 ARKISSLVKAYKNSSGTPKY c a a g g a a a a t t t c a a g t c t t g t a a a a g c a t a t a a a a a t a g t t c a g g c a c t c c g a a a t a c t g t t c c t t t t a a a g t t c a g a a c a t t t t c g t a t a t t t t t a t c a a g t c c g t g a g g c t t t a t g a

Bsu36I 420 75 80 85 90 LVGVFRNQPKEDVLALVNDY TRP1 LVGVFRNQPKEDVLALVNDY t g g t t g g c g t g t t t c g t a a t c a a c c t a a g g a g g a t g t t t t g g c t c t g g t c a a t g a t t a c g a c c a a c c g c a c a a a g c a t t a g t t g g a t t c c t c c t a c a a a a c c g a g a c c a g t t a c t a a t g c

BstXIMslIEcoRV 480 95 100 105 110 GIDIVQLHGDESWQEYQEFL TRP1 GIDIVQLHGDESWQEYQEFL g c a t t g a t a t c g t c c a a c t g c a t g g a g a t g a g t c g t g g c a a g a a t a c c a a g a g t t c c t c g c g t a a c t a t a g c a g g t t g a c g t a c c t c t a c t c a g c a c c g t t c t t a t g g t t c t c a a g g a g c

540 115 120 125 130 GLPVIKRLVFPKDCNILLSA TRP1 GLPVIKRLVFPKDCNILLSA g t t t g c c a g t t a t t a a a a g a c t c g t a t t t c c a a a a g a c t g c a a c a t a c t a c t c a g t g c a g c a a a c g g t c a a t a a t t t t c t g a g c a t a a a g g t t t t c t g a c g t t g t a t g a t g a g t c a c g t c

BspMIPaqCIBfuAIBsgI 600 135 140 145 150 ASQKPHSFIPLFDSEAGGTG TRP1 ASQKPHSFIPLFDSEAGGTG c t t c a c a g a a a c c t c a t t c g t t t a t t c c c t t g t t t g a t t c a g a a g c a g g t g g g a c a g g t g g a a g t g t c t t t g g a g t a a g c a a a t a a g g g a a c a a a c t a a g t c t t c g t c c a c c c t g t c c a c

HindIIIBmrI 660 155 160 165 170 ELLDWNSISDWVGRQESPES TRP1 ELLDWNSISDWVGRQESPES a a c t t t t g g a t t g g a a c t c g a t t t c t g a c t g g g t t g g a a g g c a a g a g a g c c c c g a a a g c t t t g a a a a c c t a a c c t t g a g c t a a a g a c t g a c c c a a c c t t c c g t t c t c t c g g g g c t t t c g a

BsaHI 720 175 180 185 190 LHFMLAGGLTPENVGDALRL TRP1 LHFMLAGGLTPENVGDALRL t a c a t t t t a t g t t a g c t g g t g g a c t g a c g c c a g a a a a t g t t g g t g a t g c g c t t a g a t t a a a t g t a a a a t a c a a t c g a c c a c c t g a c t g c g g t c t t t t a c a a c c a c t a c g c g a a t c t a a t t

780 195 200 205 210 NGVIGVDVSGGVETNGVKDS TRP1 NGVIGVDVSGGVETNGVKDS a t g g c g t t a t t g g t g t t g a t g t a a g c g g a g g t g t g g a g a c a a a t g g t g t a a a a g a c t c t a t a c c g c a a t a a c c a c a a c t a c a t t c g c c t c c a c a c c t c t g t t t a c c a c a t t t t c t g a g a t

840 215 220 225 NKIANFVKNAKK* TRP1 NKIANFVKNAKK* a c a a a a t a g c a a a t t t c g t c a a a a a t g c t a a g a a a t a g g t t a t t a c t g a g t a g t a t t t a t t g t t t t a t c g t t t a a a g c a g t t t t t a c g a t t c t t t a t c c a a t a a t g a c t c a t c a t a a a t a

End (842) 3ʹ 5ʹ 842 t t a a

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.