Addgene Hs.HRAS G13D - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: Hs.HRAS G13D


Map View

HincII - HpaI (2) BbsI (3201) AclI (3128) rrnB T2 terminator BspQI - SapI (2875) L4440 (2856 .. 2873) PciI (2758) DrdI (2656) pBR322ori-F (2603 .. 2622) BssSI - BssSαI (2585) PspFI (2458) BseYI (2454) AlwNI (2349) PaeR7I - PspXI - XhoI (2024) BsaAI (1985) BssHII (1952) BglI (1860) BtgZI (1786) M13/pUC Forward (23 .. 45) M13 Forward (37 .. 54) AhdI (50) Eco53kI (67) BanII - SacI (69) RsrII (71) BamHI (78) PsiI (153) BanI (180) PflMI (269) BsmBI - Esp3I (324) PfoI * (349) BseRI (384) FspI - FspAI (402) NcoI (514) BstXI - XcmI (521) BsgI (532) Bpu10I (574) EcoO109I - PpuMI (578) BsaI (606) AleI (660) SphI (857) HindIII (859) XbaI (871) BstEII (1388) BclI * (1426) BpmI (1480) BlpI (1537) PvuI (1609) Hs.HRAS G13D 3263 bp

Sequence View

AhdIHincIIHpaI 60 M13 fwd C C C A G T C A C G A C G T T G T A A A A C G M13/pUC Forward T G T A A A A C G A C G G C C A G T M13 Forward t t a a c g c t a g c a t g g a t g t t t t c c c a g t c a c g a c g t t g t a a a a c g a c g g c c a g t c t t a a a a a t t g c g a t c g t a c c t a c a a a a g g g t c a g t g c t g c a a c a t t t t g c t g c c g g t c a g a a t t t

BamHIEco53kIBanIISacIRsrII 120 attL1 a t t c g a g c t c g g t c c g a g g a t c c g a a a t a a t g a t t t t a t t t t g a c t g a t a g t g a c c t g t t t a a g c t c g a g c c a g g c t c c t a g g c t t t a t t a c t a a a a t a a a a c t g a c t a t c a c t g g a c a a

PsiI 180 attL1 MPTLYKKV c g t t g c a a c a a a t t g a t a a g c a a t g c t t t t t t a t a a t g c c a a c t t t g t a c a a a a a a g t t g g c a a c g t t g t t t a a c t a t t c g t t a c g a a a a a a t a t t a c g g t t g a a a c a t g t t t t t t c a a c

BanI 240 attL1 GTMTEYKLVVVGAGDVGKSA g c a c c a t g a c t g a g t a c a a a c t g g t g g t g g t t g g a g c a g g t g a t g t t g g g a a a a g c g c a c c g t g g t a c t g a c t c a t g t t t g a c c a c c a c c a a c c t c g t c c a c t a c a a c c c t t t t c g c g t g

PflMI 300 LTIQLIQNHFVDEYDPTIED t g a c c a t c c a g c t g a t c c a g a a c c a t t t t g t g g a c g a a t a c g a c c c c a c t a t a g a g g a t t a c t g g t a g g t c g a c t a g g t c t t g g t a a a a c a c c t g c t t a t g c t g g g g t g a t a t c t c c t a a

PfoI*BsmBIEsp3I 360 SYRKQVVIDGETCLLDILDT c c t a c c g g a a g c a g g t g g t c a t t g a t g g g g a g a c g t g c c t g t t g g a c a t c c t g g a t a c c g g g a t g g c c t t c g t c c a c c a g t a a c t a c c c c t c t g c a c g g a c a a c c t g t a g g a c c t a t g g c

FspAIFspIBseRI 420 AGQEEYSAMRDQYMRTGEGF c c g g c c a g g a g g a g t a c a g c g c c a t g c g g g a c c a g t a c a t g c g c a c c g g g g a g g g c t t c c g g c c g g t c c t c c t c a t g t c g c g g t a c g c c c t g g t c a t g t a c g c g t g g c c c c t c c c g a a g g

480 LCVFAINNTKSFEDIHQYRE t g t g t g t g t t t g c c a t c a a c a a c a c c a a g t c t t t t g a g g a c a t c c a c c a g t a c a g g g a g c a c a c a c a c a a a c g g t a g t t g t t g t g g t t c a g a a a a c t c c t g t a g g t g g t c a t g t c c c t c g

BsgIXcmIBstXINcoI 540 QIKRVKDSDDVPMVLVGNKC a g a t c a a a c g g g t g a a g g a c t c g g a t g a c g t g c c c a t g g t g c t g g t g g g g a a c a a g t g t g t c t a g t t t g c c c a c t t c c t g a g c c t a c t g c a c g g g t a c c a c g a c c a c c c c t t g t t c a c a c

Bpu10IEcoO109IPpuMI 600 DLAARTVESRQAQDLARSYG a c c t g g c t g c a c g c a c t g t g g a a t c t c g g c a g g c t c a g g a c c t c g c c c g a a g c t a c g g c a t g g a c c g a c g t g c g t g a c a c c t t a g a g c c g t c c g a g t c c t g g a g c g g g c t t c g a t g c c g t

AleIBsaI 660 IPYIETSAKTRQGVEDAFYT t c c c c t a c a t c g a g a c c t c g g c c a a g a c c c g g c a g g g a g t g g a g g a t g c c t t c t a c a c g t a g g g g a t g t a g c t c t g g a g c c g g t t c t g g g c c g t c c c t c a c c t c c t a c g g a a g a t g t g c a

720 LVREIRQHKLRKLNPPDESG t g g t g c g t g a g a t c c g g c a g c a c a a g c t g c g g a a g c t g a a c c c t c c t g a t g a g a g t g g c c a c c a c g c a c t c t a g g c c g t c g t g t t c g a c g c c t t c g a c t t g g g a g g a c t a c t c t c a c c g g

780 attL2 PGCMSCKCVLS* c c g g c t g c a t g a g c t g c a a g t g t g t g c t c t c c t a a t c a a c t t t c t t g t a c a a a g t t g g c a g g c c g a c g t a c t c g a c g t t c a c a c a c g a g a g g a t t a g t t g a a a g a a c a t g t t t c a a c c g t

840 attL2 t t a t a g g a a a g c a t t g c t t a t c a a t t t g t t g c a a c g a a c a g g t c a c t a t c a g t c a a a a t a a a t a t c c t t t c g t a a c g a a t a g t t a a a c a a c g t t g c t t g t c c a g t g a t a g t c a g t t t t a t

XbaISphIHindIII 900 attL2 a a a t c a t t a t t t g c a t g c a a g c t t a g a t c c t c t a g a c c a g c c a g g a c a g a a a t g c c t c g a t t t a g t a a t a a a c g t a c g t t c g a a t c t a g g a g a t c t g g t c g g t c c t g t c t t t a c g g a g c t

960 c t t c g c t g c t a c c c a a g g t t g c c g g g t g a c g c a c a c c g t g g a a a c g g a t g a a g g c a c g a a g a a g c g a c g a t g g g t t c c a a c g g c c c a c t g c g t g t g g c a c c t t t g c c t a c t t c c g t g c t t

1020 MRS c c c a g t g g a c a t a a g c c t g t t c g g t t c g t a a g c t g t a a t g c a a g t a g c g t a t g c g c t c a c g g g t c a c c t g t a t t c g g a c a a g c c a a g c a t t c g a c a t t a c g t t c a t c g c a t a c g c g a g t g

1080 RNWSRTLTERSGGNGAVAVF g c a a c t g g t c c a g a a c c t t g a c c g a a c g c a g c g g t g g t a a c g g c g c a g t g g c g g t t t t c a c g t t g a c c a g g t c t t g g a a c t g g c t t g c g t c g c c a c c a t t g c c g c g t c a c c g c c a a a a g t

1140 MACYDCFFGVQSMPRASKQQ t g g c t t g t t a t g a c t g t t t t t t t g g g g t a c a g t c t a t g c c t c g g g c a t c c a a g c a g c a a g a c c g a a c a a t a c t g a c a a a a a a a c c c c a t g t c a g a t a c g g a g c c c g t a g g t t c g t c g t t c

1200 ARYAVGRCLMLWSSNDVTQQ c g c g t t a c g c c g t g g g t c g a t g t t t g a t g t t a t g g a g c a g c a a c g a t g t t a c g c a g c a g g g c g c a a t g c g g c a c c c a g c t a c a a a c t a c a a t a c c t c g t c g t t g c t a c a a t g c g t c g t c c

1260 1 5 10 MREAVIAEVS SmR GSRPKTKLNIMREAVIAEVS g c a g t c g c c c t a a a a c a a a g t t a a a c a t t a t g a g g g a a g c g g t g a t c g c c g a a g t a t c g a c g t c a g c g g g a t t t t g t t t c a a t t t g t a a t a c t c c c t t c g c c a c t a g c g g c t t c a t a g c t

1320 15 20 25 30 TQLSEVVGVIERHLEPTLLA SmR TQLSEVVGVIERHLEPTLLA c t c a a c t a t c a g a g g t a g t t g g c g t c a t c g a g c g c c a t c t c g a a c c g a c g t t g c t g g c c g g a g t t g a t a g t c t c c a t c a a c c g c a g t a g c t c g c g g t a g a g c t t g g c t g c a a c g a c c g g c

1380 35 40 45 50 VHLYGSAVDGGLKPHSDIDL SmR VHLYGSAVDGGLKPHSDIDL t a c a t t t g t a c g g c t c c g c a g t g g a t g g c g g c c t g a a g c c a c a c a g t g a t a t t g a t t t g c a t g t a a a c a t g c c g a g g c g t c a c c t a c c g c c g g a c t t c g g t g t g t c a c t a t a a c t a a a c g

BclI*BstEII 1440 55 60 65 70 LVTVTVRLDETTRRALINDL SmR LVTVTVRLDETTRRALINDL t g g t t a c g g t g a c c g t a a g g c t t g a t g a a a c a a c g c g g c g a g c t t t g a t c a a c g a c c t t t a c c a a t g c c a c t g g c a t t c c g a a c t a c t t t g t t g c g c c g c t c g a a a c t a g t t g c t g g a a a

BpmI 1500 75 80 85 90 LETSASPGESEILRAVEVTI SmR LETSASPGESEILRAVEVTI t g g a a a c t t c g g c t t c c c c t g g a g a g a g c g a g a t t c t c c g c g c t g t a g a a g t c a c c a t t g a c c t t t g a a g c c g a a g g g g a c c t c t c t c g c t c t a a g a g g c g c g a c a t c t t c a g t g g t a a c

BlpI 1560 95 100 105 110 VVHDDIIPWRYPAKRELQFG SmR VVHDDIIPWRYPAKRELQFG t t g t g c a c g a c g a c a t c a t t c c g t g g c g t t a t c c a g c t a a g c g c g a a c t g c a a t t t g g a g a a c a c g t g c t g c t g t a g t a a g g c a c c g c a a t a g g t c g a t t c g c g c t t g a c g t t a a a c c t c

PvuI 1620 115 120 125 130 EWQRNDILAGIFEPATIDID SmR EWQRNDILAGIFEPATIDID a a t g g c a g c g c a a t g a c a t t c t t g c a g g t a t c t t c g a g c c a g c c a c g a t c g a c a t t g a t c t t a c c g t c g c g t t a c t g t a a g a a c g t c c a t a g a a g c t c g g t c g g t g c t a g c t g t a a c t a g

1680 135 140 145 150 LAILLTKAREHSVALVGPAA SmR LAILLTKAREHSVALVGPAA t g g c t a t c t t g c t g a c a a a a g c a a g a g a a c a t a g c g t t g c c t t g g t a g g t c c a g c g g c g g a c c g a t a g a a c g a c t g t t t t c g t t c t c t t g t a t c g c a a c g g a a c c a t c c a g g t c g c c g c c

1740 155 160 165 170 EELFDPVPEQDLFEALNETL SmR EELFDPVPEQDLFEALNETL a g g a a c t c t t t g a t c c g g t t c c t g a a c a g g a t c t a t t t g a g g c g c t a a a t g a a a c c t t a a t c c t t g a g a a a c t a g g c c a a g g a c t t g t c c t a g a t a a a c t c c g c g a t t t a c t t t g g a a t t

BtgZI 1800 175 180 185 190 TLWNSPPDWAGDERNVVLTL SmR TLWNSPPDWAGDERNVVLTL c g c t a t g g a a c t c g c c g c c c g a c t g g g c t g g c g a t g a g c g a a a t g t a g t g c t t a c g t t g t g c g a t a c c t t g a g c g g c g g g c t g a c c c g a c c g c t a c t c g c t t t a c a t c a c g a a t g c a a c a

BglI 1860 195 200 205 210 SRIWYSAVTGKIAPKDVAAD SmR SRIWYSAVTGKIAPKDVAAD c c c g c a t t t g g t a c a g c g c a g t a a c c g g c a a a a t c g c g c c g a a g g a t g t c g c t g c c g a c t g g g c g t a a a c c a t g t c g c g t c a t t g g c c g t t t t a g c g c g g c t t c c t a c a g c g a c g g c t g a

1920 215 220 225 230 WAMERLPAQYQPVILEARQA SmR WAMERLPAQYQPVILEARQA g g g c a a t g g a g c g c c t g c c g g c c c a g t a t c a g c c c g t c a t a c t t g a a g c t a g a c a g g c t t c c c g t t a c c t c g c g g a c g g c c g g g t c a t a g t c g g g c a g t a t g a a c t t c g a t c t g t c c g a a

BssHII 1980 235 240 245 250 YLGQEEDRLASRADQLEEFV SmR YLGQEEDRLASRADQLEEFV a t c t t g g a c a a g a a g a a g a t c g c t t g g c c t c g c g c g c a g a t c a g t t g g a a g a a t t t g t c c t a g a a c c t g t t c t t c t t c t a g c g a a c c g g a g c g c g c g t c t a g t c a a c c t t c t t a a a c a g g

PaeR7IPspXIXhoIBsaAI 2040 255 260 HYVKGEITKVVGK* SmR HYVKGEITKVVGK* a c t a c g t g a a a g g c g a g a t c a c c a a g g t a g t c g g c a a a t a a c c c t c g a g c c a c c c a t g a c t g a t g c a c t t t c c g c t c t a g t g g t t c c a t c a g c c g t t t a t t g g g a g c t c g g t g g g t a c t g

2100 c a a a a t c c c t t a a c g t g a g t t a c g c g t c g t t c c a c t g a g c g t c a g a c c c c g t a g a a a a g a g t t t t a g g g a a t t g c a c t c a a t g c g c a g c a a g g t g a c t c g c a g t c t g g g g c a t c t t t t c t

2160 ori t c a a a g g a t c t t c t t g a g a t c c t t t t t t t c t g c g c g t a a t c t g c t g c t t g c a a a c a a a a a a g t t t c c t a g a a g a a c t c t a g g a a a a a a a g a c g c g c a t t a g a c g a c g a a c g t t t g t t t t t

2220 ori a a c c a c c g c t a c c a g c g g t g g t t t g t t t g c c g g a t c a a g a g c t a c c a a c t c t t t t t c c g a t t g g t g g c g a t g g t c g c c a c c a a a c a a a c g g c c t a g t t c t c g a t g g t t g a g a a a a a g g c t

2280 ori a g g t a a c t g g c t t c a g c a g a g c g c a g a t a c c a a a t a c t g t c c t t c t a g t g t a g c c g t a g t t c c a t t g a c c g a a g t c g t c t c g c g t c t a t g g t t t a t g a c a g g a a g a t c a c a t c g g c a t c a

2340 ori t a g g c c a c c a c t t c a a g a a c t c t g t a g c a c c g c c t a c a t a c c t c g c t c t g c t a a t c c t g t a t c c g g t g g t g a a g t t c t t g a g a c a t c g t g g c g g a t g t a t g g a g c g a g a c g a t t a g g a c a

AlwNI 2400 ori t a c c a g t g g c t g c t g c c a g t g g c g a t a a g t c g t g t c t t a c c g g g t t g g a c t c a a g a c g a t a t g g t c a c c g a c g a c g g t c a c c g c t a t t c a g c a c a g a a t g g c c c a a c c t g a g t t c t g c t a

BseYIPspFI 2460 ori a g t t a c c g g a t a a g g c g c a g c g g t c g g g c t g a a c g g g g g g t t c g t g c a c a c a g c c c a g c t t c a a t g g c c t a t t c c g c g t c g c c a g c c c g a c t t g c c c c c c a a g c a c g t g t g t c g g g t c g a

2520 ori t g g a g c g a a c g a c c t a c a c c g a a c t g a g a t a c c t a c a g c g t g a g c a t t g a g a a a g c g c c a a c c t c g c t t g c t g g a t g t g g c t t g a c t c t a t g g a t g t c g c a c t c g t a a c t c t t t c g c g g t

2580 ori c g c t t c c c g a a g g g a g a a a g g c g g a c a g g t a t c c g g t a a g c g g c a g g g t c g g a a c a g g a g g c g a a g g g c t t c c c t c t t t c c g c c t g t c c a t a g g c c a t t c g c c g t c c c a g c c t t g t c c t c

BssSαIBssSI 2640 ori G G G A A A C G C C T G G T A T C T T T pBR322ori-F a g c g c a c g a g g g a g c t t c c a g g g g g a a a c g c c t g g t a t c t t t a t a g t c c t g t c g g g t t t c t c g c g t g c t c c c t c g a a g g t c c c c c t t t g c g g a c c a t a g a a a t a t c a g g a c a g c c c a a a g

DrdI 2700 ori g c c a c c t c t g a c t t g a g c g t c g a t t t t t g t g a t g c t c g t c a g g g g g g c g g a g c c t a t g g a c g g t g g a g a c t g a a c t c g c a g c t a a a a a c a c t a c g a g c a g t c c c c c c g c c t c g g a t a c c t

PciI 2760 ori a a a a c g c c a g c a a c g c g g c c t t t t t a c g g t t c c t g g c c t t t t g c t g g c c t t t t g c t c a c a t t t t g c g g t c g t t g c g c c g g a a a a a t g c c a a g g a c c g g a a a a c g a c c g g a a a a c g a g t g t

2820 t g t t c t t t c c t g c g t t a t c c c c t g a t t c t g t g g a t a a c c g t a t t a c c g c c t t t g a g t g a g a c a a g a a a g g a c g c a a t a g g g g a c t a a g a c a c c t a t t g g c a t a a t g g c g g a a a c t c a c t c

BspQISapI 2880 A G C G A G T C A G T G A G C G A G L4440 c t g a t a c c g c t c g c c g c a g c c g a a c g a c c g a g c g c a g c g a g t c a g t g a g c g a g g a a g c g g g a c t a t g g c g a g c g g c g t c g g c t t g c t g g c t c g c g t c g c t c a g t c a c t c g c t c c t t c g c c

2940 a a g a g c g c c c a a t a c g c a a a c c g c c t c t c c c c g c g c g t t g g c c g a t t c a t t a a t g c a g c t t t c t c g c g g g t t a t g c g t t t g g c g g a g a g g g g c g c g c a a c c g g c t a a g t a a t t a c g t c g a

3000 g g c a c g a c a g g t t t c c c g a c t g g a a a g c g g g c a g t g a g c g c a a c g c a a t t a a t a c g c g t a c c g t g c t g t c c a a a g g g c t g a c c t t t c g c c c g t c a c t c g c g t t g c g t t a a t t a t g c g c a t

3060 rrnB T2 terminator c c g c t a g c c a g g a a g a g t t t g t a g a a a c g c a a a a a g g c c a t c c g t c a g g a t g g c c t t c t g g g c g a t c g g t c c t t c t c a a a c a t c t t t g c g t t t t t c c g g t a g g c a g t c c t a c c g g a a g a c

3120 c t t a g t t t g a t g c c t g g c a g t t t a t g g c g g g c g t c c t g c c c g c c a c c c t c c g g g c c g t t g g a a t c a a a c t a c g g a c c g t c a a a t a c c g c c c g c a g g a c g g g c g g t g g g a g g c c c g g c a a c

AclI 3180 rrnB T1 terminator c t t c a c a a c g t t c a a a t c c g c t c c c g g c g g a t t t g t c c t a c t c a g g a g a g c g t t c a c c g a g a a g t g t t g c a a g t t t a g g c g a g g g c c g c c t a a a c a g g a t g a g t c c t c t c g c a a g t g g c t

BbsI 3240 rrnB T1 terminator c a a a c a a c a g a t a a a a c g a a a g g c c c a g t c t t c c g a c t g a g c c t t t c g t t t t a t t t g a t g g t t t g t t g t c t a t t t t g c t t t c c g g g t c a g a a g g c t g a c t c g g a a a g c a a a a t a a a c t a c

3263 c c t g g c a g t t c c c t a c t c t c g c g g g a c c g t c a a g g g a t g a g a g c g c

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.