Addgene pcDNA3.1-MS2-Tet1-CD Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pcDNA3.1-MS2-Tet1-CD Sequencing Result

Map View

800 600 400 200 End (912) AcuI (871) BclI * (832) BsiEI (820) SapI - BspQI (792) BspEI - BsaWI (760) MscI (742) EcoP15I (681) DraIII (636) MflI * BstYI BamHI (615) BsgI (613) MspA1I (583) BpmI (529) HaeII (508) AfeI (506) BtgZI (464) NmeAIII (454) BsrDI (442) MmeI (436) ApoI - EcoRI (407) BstXI - XcmI (330) FspI (274) DrdI (171) EciI (169) BmgBI (167) HincII (146) SalI (144) BtgI - NcoI - KpnI (112) Acc65I (108) AflII - SmlI (99) DraI - PmeI (95) BmtI (90) NheI (86) T7 (54 .. 73) AseI (53) BtsI - BtsαI (29) Start (0) T7 promoter MS2 SV40 NLS MS2-N55K pcDNA3.1-MS2-Tet1-CD 912 bp

Sequence View

AseIBtsαIBtsIStart (0) 5ʹ 3ʹ 60 T7 promoter T A A T A C G T7 a g c a g a g c t c t c t g g c t a a c t a g a g a a c c c a c t g c t t a c t g g c t t a t c g a a a t t a a t a c g t c g t c t c g a g a g a c c g a t t g a t c t c t t g g g t g a c g a a t g a c c g a a t a g c t t t a a t t a t g c

Acc65IKpnINcoIBtgINheIBmtIPmeIDraISmlIAflII 120 1 A T7 promoter MS2 1 A MS2-N55K A C T C A C T A T A G G G T7 MA a c t c a c t a t a g g g a g a c c c a a g c t g g c t a g c g t t t a a a c t t a a g c t t g g t a c c a t g g c t t t g a g t g a t a t c c c t c t g g g t t c g a c c g a t c g c a a a t t t g a a t t c g a a c c a t g g t a c c g a a

BmgBIEciIDrdISalIHincII 180 5 10 15 20 SNFTQFVLVDNGGTGDVTVA MS2 5 10 15 20 SNFTQFVLVDNGGTGDVTVA MS2-N55K SNFTQFVLVDNGGTGDVTVA c t a a c t t t a c t c a g t t c g t t c t c g t c g a c a a t g g c g g a a c t g g c g a c g t g a c t g t c g c c c g a t t g a a a t g a g t c a a g c a a g a g c a g c t g t t a c c g c c t t g a c c g c t g c a c t g a c a g c g g g

240 25 30 35 40 PSNFANGVAEWISSNSRSQA MS2 25 30 35 40 PSNFANGVAEWISSNSRSQA MS2-N55K PSNFANGVAEWISSNSRSQA c a a g c a a c t t c g c t a a c g g g g t c g c t g a a t g g a t c a g c t c t a a c t c g c g t t c a c a g g c t t g t t c g t t g a a g c g a t t g c c c c a g c g a c t t a c c t a g t c g a g a t t g a g c g c a a g t g t c c g a a

FspI 300 45 50 55 60 YKVTCSVRQSSAQKRKYTIK MS2 45 50 55 60 YKVTCSVRQSSAQKRKYTIK MS2-N55K YKVTCSVRQSSAQKRKYTIK a c a a a g t a a c c t g t a g c g t t c g t c a g a g c t c t g c g c a g a a g c g c a a a t a c a c c a t c a a a g t g t t t c a t t g g a c a t c g c a a g c a g t c t c g a g a c g c g t c t t c g c g t t t a t g t g g t a g t t t c

XcmIBstXI 360 65 70 75 80 VEVPKVATQTVGGVELPVAA MS2 65 70 75 80 VEVPKVATQTVGGVELPVAA MS2-N55K VEVPKVATQTVGGVELPVAA t c g a g g t g c c t a a a g t g g c a a c c c a g a c t g t t g g t g g t g t a g a g c t t c c t g t a g c c g c a t a g c t c c a c g g a t t t c a c c g t t g g g t c t g a c a a c c a c c a c a t c t c g a a g g a c a t c g g c g t a

EcoRIApoI 420 85 90 95 100 WRSYLNMELTIPIFATNSDC MS2 85 90 95 100 WRSYLNMELTIPIFATNSDC MS2-N55K WRSYLNMELTIPIFATNSDC g g c g t t c g t a c t t a a a t a t g g a a c t a a c c a t t c c a a t t t t c g c c a c g a a t t c c g a c t g c g c c g c a a g c a t g a a t t t a t a c c t t g a t t g g t a a g g t t a a a a g c g g t g c t t a a g g c t g a c g c

BtgZINmeAIIIBsrDIMmeI 480 105 110 115 120 ELIVKAMQGLLKDGNPIPSA MS2 105 110 115 120 ELIVKAMQGLLKDGNPIPSA MS2-N55K ELIVKAMQGLLKDGNPIPSA a g c t t a t t g t t a a g g c a a t g c a a g g t c t c c t a a a a g a t g g a a a c c c g a t t c c c t c g g c c a t c g a a t a a c a a t t c c g t t a c g t t c c a g a g g a t t t t c t a c c t t t g g g c t a a g g g a g c c g g t

BpmIAfeIHaeII 540 125 IAANSGIY (in frame with MS2) SAGGGGSGGGGS MS2 125 IAANSGIY (in frame with MS2-N55K) SAGGGGSGGGGS MS2-N55K IAANSGIYSAGGGGSGGGGS t c g c a g c a a a c t c c g g c a t c t a c a g c g c t g g a g g a g g t g g a a g c g g a g g a g g a g g a a g c g a g c g t c g t t t g a g g c c g t a g a t g t c g c g a c c t c c t c c a c c t t c g c c t c c t c c t c c t t c g c

MspA1I 600 (in frame with MS2) GGGGSPKKKRKVEASGGGSG GGGGS 130 PKKKRKV (in frame with SV40 NLS) EASGGGSG SV40 NLS GGGGSPKKKRKVEASGGGSG g a g g a g g a g g t a g c c c c a a g a a g a a g a g a a a g g t g g a g g c c a g c g g t g g a g g a a g t g g a g c t c c t c c t c c a t c g g g g t t c t t c t t c t c t t t c c a c c t c c g g t c g c c a c c t c c t t c a c c t c

DraIIIBsgIBamHIBstYIMflI* 660 (in frame with MS2) GGSGGGSGEAAPCDCDGGTQ (in frame with SV40 NLS) GGSGGGSGEAAPCDCDGGTQ GGSGGGSGEAAPCDCDGGTQ g t g g t t c a g g t g g a g g a t c c g g c g a a g c t g c a c c c t g t g a c t g t g a t g g a g g t a c a c a a a c a c c a a g t c c a c c t c c t a g g c c g c t t c g a c g t g g g a c a c t g a c a c t a c c t c c a t g t g t t t

EcoP15I 720 (in frame with MS2) KEKGPYYTHLGAGPSVAAVR (in frame with SV40 NLS) KEKGPYYTHLGAGPSVAAVR KEKGPYYTHLGAGPSVAAVR a a g a a a a a g g c c c a t a t t a t a c a c a c c t t g g g g c a g g a c c a a g t g t g g c t g c t g t c a g g g t t c t t t t t c c g g g t a t a a t a t g t g t g g a a c c c c g t c c t g g t t c a c a c c g a c g a c a g t c c c

BsaWIBspEIMscI 780 (in frame with MS2) ELMETRFGQKGKAIRIEKIV (in frame with SV40 NLS) ELMETRFGQKGKAIRIEKIV ELMETRFGQKGKAIRIEKIV a g c t c a t g g a g a c t a g g t t t g g c c a g a a g g g g a a g g c a a t c c g g a t t g a g a a g a t a g t g t t c g a g t a c c t c t g a t c c a a a c c g g t c t t c c c c t t c c g t t a g g c c t a a c t c t t c t a t c a c a

BclI*BsiEIBspQISapI 840 (in frame with MS2) FTGKEGKSSQGCPVAKWVIR (in frame with SV40 NLS) FTGKEGKSSQGCPVAKWVIR FTGKEGKSSQGCPVAKWVIR t c a c g g g g a a g g a a g g g a a g a g c t c t c a g g g c t g c c c g g t c g c c a a g t g g g t g a t c a g a a a g t g c c c c t t c c t t c c c t t c t c g a g a g t c c c g a c g g g c c a g c g g t t c a c c c a c t a g t c t t

AcuI 900 (in frame with MS2) RSGPEEKLICLVRERVDHHC (in frame with SV40 NLS) RSGPEEKLICLVRERVDHHC RSGPEEKLICLVRERVDHHC g a a g t g g t c c t g a a g a g a a g c t t a t t t g t t t g g t t c g t g a g c g t g t a g a c c a t c a c t g t t c t t c a c c a g g a c t t c t c t t c g a a t a a a c a a a c c a a g c a c t c g c a c a t c t g g t a g t g a c a a

End (912) 3ʹ 5ʹ 912 STAV STAV STAV c g a c g g c t g t g a g c t g c c g a c a c t

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.